Detailed Identification of Fatty Acid Isomers

Sheds Light on the Probable Precursors of

Triacylglycerol Accumulation in
Photoautotrophically Grown Chlamydomonas
reinhardtii
Kenta Sakurai, Takashi Moriyama and Naoki Sato
13 Dec 13

MATERIALS AND METHODS

Algal strain.
The green alga Chlamydomonas reinhardtii strain CC1010 was obtained
from the Chlamydomonas Resource Center (St. Paul, MN).

Growth conditions.
The algal cells were grown at 25C in flat culture flasks (capacity, 500 ml
with air space) containing 500 ml of modified Bristol's medium (MBM)
(Watanabe A. et al., 1960) with continuous aeration by 1% CO2 in air.
In some experiments, aeration was provided by either 5% CO2 in air or
ordinary air. Light (45 mol m2 s1) was provided by a bank of white
fluorescent tubes (color temperature, 6,700 K; model FL20S FR P;
Panasonic, Osaka, Japan). MBM contained, per liter, 250 mg KNO3, 7.5
mg MgSO47H2O, 25 mg NaCl, 7.5 mg K2HPO4, 175 mg KH2PO4, 13 mg
Ca(NO3)4H2O, 2 ml Fe stock solution, and 1 ml A5 solution (the pH was
adjusted to 6.5 with 1 N NaOH). The Fe stock solution contained 0.5 g
liter1 FeSO47H2O, whereas the A5 solution contained, per liter, 2.8 g
H3BO3, 2.5 g MnSO47H2O, 0.2 g ZnSO47H2O, 0.07 g CuSO45H2O, and
0.02 g Na2MoO42H2O. KNO3 was not added in the nitrogen-depleted
medium, which we designated MBM-noN; it included 10 mg
liter1 CaCl22H2O instead of Ca(NO3)4H2O.