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  • Plantextracts

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    Plant antimicrobial agents can be tested against many viruses. If proven safe for humans can be used to help kill the microbes that have infected the body.. The purpose of our project is to test whether the plant will use defensive antimicrobials.

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    plantextracts

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    Plant antimicrobial agents can be tested against many viruses. If proven safe for humans can be used to help kill the microbes that have infected the body.. The purpose of our project is to test whether the plant will use defensive antimicrobials.

    Авторское право:

    © All Rights Reserved
    Скачайте в формате PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    24 просмотров5 страниц

    Plantextracts

    Загружено:

    api-272956648
    Plant antimicrobial agents can be tested against many viruses. If proven safe for humans can be used to help kill the microbes that have infected the body.. The purpose of our project is to test whether the plant will use defensive antimicrobials.

    Авторское право:

    © All Rights Reserved
    Скачайте в формате PDF, TXT или читайте онлайн в Scribd
    Отметить как неприемлемый контент
    из 5

    PatrickHeslip

    EthanDanziger
    SimoneSwetland

    TestingPlantSubstanceasPotentialMedicines

    Background:Plantsarealwaysbattlingforsurvivalinthewildespecially
    againstdiseases.Duetothistheyhavedevelopedantimicrobialagentsto
    defendagainstthemicrobes.Ifisolatedtheseantimicrobialagentscanbe
    testedagainstmanyviruses.Thenifprovensafeforhumanscanbeused
    tohelpkillthemicrobesthathaveinfectedthebody.

    Purpose/Objective:Thepurposeofourprojectistotestwhethertheplant
    willusedefensiveantimicrobials.Thenwewillseewhatspecific
    antimicrobialsitwillusetofendoffthebacteriagrowth.

    Materials:
    Balance,weighboat,labscoops
    LBbrothbase
    Mediabottles,250mL
    Sterilizer/autoclave
    Waterbath,37,shaking
    SterileLBagar
    Laminarflowhoodanddisinfectant
    Glasses,safety,plastic
    Bunsenburnerandgaslighter
    Inoculatingloop,Ni/Crwire
    Petridishes,60X15mm,sterile
    E.coli
    JM109(stockplate)
    Plantspecimen
    Mortarandpestle
    Pipet,10mLandpump
    Plasticfunnelsshortstemmed

    Filterpaperdisks,5mmdiameter
    Beakers,100mL
    Syringe,10mLandfilter,0.2um
    Reactiontubesandrack,1.7mL
    Methanol,absolute
    Pipet,1mLandpump
    Dryblockheater/heatblock
    Forceps,finetipped
    Ampicillin
    Glassspreader
    Incubatoroven,37
    Lemonleaves

    Procedure:
    measure2gramsofplantmaterial
    usingamortarandpestle,grindup2gramsofplanttissuewith10
    millilitersofdeionizedwater
    letsitfor3minutes
    filterthesamplethroughan11centimeterfilterpaperfunnel
    filtersterilizetheextractusingasyringefilter
    collect1milliliterofextractina1.7millilitermicrotube.Labelthetube
    Repeatthestepsusingmethanolinsteadofwater
    FilterSterilization(procedure)
    AttachprefittertosyringeandrinsewithH2O
    Carefullyopensterilizationfilter,keepingthefilterinitsplastic
    covering
    Loadapprox1.5mLofH2Obasedfiltrateusingapipet
    Depresstheplunger,Collectingthesterilefilteredfiltrateinto
    microfugetube
    Quicklyclosemicrofugecapuntilasnapsoundisheard
    useflamesterilizationforcestotransferfiltersquaresintoH2O
    controlandampicillincontrol
    labelplate(bottom)

    dispense1mLE.coliineachplate
    Flamesterilespreadingloop
    placefilterdiscsinplate

    Results:

    MethanolExtract:
    24hrs.

    48hrs.

    WaterExtract:
    24hrs.

    48hrs.

    24hours

    48hours

    MethanolExtract

    Ampicillinpaperdisc
    hasanantimicrobial
    ringarounditandthe
    plantextractsdont
    havethering

    Ampicillinpaperdisc
    stillhasantimicrobial
    ringarounditandthe
    plantextractsare
    startingtohave
    antimicrobialring.
    Therearewhitedots
    onoursolution
    becauseofcross
    contamination.

    WaterExtract

    Ampicillinpaperdisc
    hasanantimicrobial
    ringarounditandthe
    plantextractsdont
    havethering

    Ampicillinpaperdisc
    stillhasantimicrobial
    ringarounditandthe
    plantextractsare
    startingtohave
    antimicrobialring.
    Therearewhitedots
    onoursolution
    becauseofcross
    contamination.

    Analysis:

    Ourmethanolextractandourplantextractbothendeduphavingan
    antimicrobialringaroundit.Ourampicillinringalsohadanantimicrobial
    ringaroundit.Wecouldtellbecausetherewasaclearcirclearoundthe
    piecesofpaper.Unfortunatelyourtraysgotalittlecontaminatedduringthe
    lab.Wehadwhitedotsonthebacteriafieldthatwasinthetray,butthat
    didntaffecttheendresultsoftheproject.

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