Outline for thesis :

Chapter 1: Introduction
1.1 Background of the study
1.1.1 Breast cancer
1.1.2 Herbal medicine
1.1.2.1 Ginger
1.1.3 Ultrasound Wave
1.1.3.1 High Intensity frequency Ultrasound
1.1.3.2 Low Intensity Pulse Ultrasound
1.2 Objectives of the Study
1.3 Scope and Limitation
1.4 Significant of the study
Chapter 2: Related Review of Literature
2.1 Breast cancer
2.1.1 Anatomy of a Normal Breast
2.1.2 Types of Breast Cancer
2.1.3 Breast cancer cell line
2.2 Ginger
2.2.1 Chemical components of ginger
2.2.1.2 Benifits of Chemical components of ginger
2.2.2 Molecular target of Ginger
2.2.3 Benifits of Ginger
2.2.3.1Anti-Tumor activity of ginger
2.2.3.2 Anti inflammatory activity of ginger
2.2.3.3 Tissue and radio-protective effects of ginger
2.3 Ultrasound
2.3.1
2.3.2

High Intensity frequency Ultrasound

Low Intensity Pulse Ultrasound

Chapter 3. Theoretical Background

3.4 Cell Viability Tests
3.4.1 AlamarBlue
3.4.2 Spectrophotometer and Beers Law
3.5 Statistical Analysis
3.5.1 t-Test 25
3.5.2 Three-way Analysis of Variance (ANOVA)
Chapter 4: Methodology
4.1 Construction of Ultrasound Source Setup
4.2 Sample Preparation of Escherichia coli
4.3 Treatment Setup
4.3.1 Control Group
4.3.2 Treatment via Modulated Ultrasound
4.3.3 Treatment via Modulated Rife Frequency
4.3.4 Ultrasound-Radio Frequency Tandem Treatment
4.4 Cytotoxicity test
4.4.1 AlamarBlue Dye Distribution
4.4.2 Spectrophotometer
4.5 Statistical Analysis
Chapter 5: Expected Results
5.1 Sample Data for Expected Results

Appendix;
A1 Budget Plan
A2 Gantt Chart
A3 References