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Biological Motivations for

Tensegrity Structures
UCSD 2009 - Cluster No. 7

Team Members
Topic #1) Bioengineering: The Amazing Red Blood Cells

CarlosVeraM.D.,Ph.D.,BioengineeringProjectScientist/Lecturer.
TerrellGreenM.S.,GraduateStudent,Bioengineering.

Topic #2) Structures and Control: Modeling the Red


Blood Cell Membrane Skeleton using Tensegrity

RobertSkeltonPh.D.,ProfessorEmeritusofMechanicalandAerospaceEngineering.
MauriciodeOliveiraPh.D.,AdjunctProfessorofMechanicalandAerospace
, j
p
Engineering.
DanielCorbalan,GraduateStudent,MechanicalandAerospaceEngineering.
Teacher Fellow: DouglasBlackington
Douglas Blackington B.A.,M.S.,PacificRidgeSchool.
B A M S Pacific Ridge School

Description
Engineering plays an increasingly important role in
medicine through projects that range from basic
research in physiology to advances in biotechnology
and the improvement of health care.
Bioengineering employs the principles and tools of
traditional engineering fields to solve biomedical
problems.
We will explore how bioengineering can be used to
study the structure and function of the Red Blood
Cells.

Topic No.
No 1
Bioengineering: The Amazing
Red Blood Cells

TheBookofNaturemayindeedbewrittenin
Th
B k fN
i d db
i
i
thecharactersofgeometry
Platon/Galileo/DArcyW.Thompson

E-Tmod Knockout Mouse

Xin Chu, UCSD Bioengineering

Pipette Handling
g

Mice Blood Smear

COSMOS @ UCSD Bioengineering

Osmotic Response

Ghost Membranes

Hypotonic
lysis
RBC

COSMOS @ UCSD Bioengineering

wash
Hemoglobin
and cytosolic
proteins

Ghost
membrane

SDS-PAGE Protein Pattern


Band
1
2
2.1

Ghost membrane
Heat with SDS &
mercaptoethanol

Total proteins
Electrophoresis

Coomassie
Blue staining

3
4.1
42
4.2
5
6
7

COSMOS @ UCSD Bioengineering

10

Western Blot

G.M.

p tissues
Multiple

64 kDa

30 kDa
26 kDa

COSMOS @ UCSD Bioengineering

12

E-Tmod Knockout Model

Genotyping
Wt: 1.7 Kb
KO: 2.2 Kb
Chu et al., 2003

Design a Novel Mechanical Test

Topic No. 2
Structures and Control:
Modeling the Red Blood Cell
Membrane Skeleton using
Tensegrity
g y

Tensegrity

3-D Nanomechanics of RBC


Junctional Complex
Collaboration between Bob Skelton
and Amy Sung Laboratories

(Vera et al
al., 2005)

3D Protein
Network
Simulation

Mauricio de Oliveira et. al., 2009.

Tensegrity:ScalableTechnology
From Nano toMegaScale
FromNano
to Mega Scale

Blood
cells

Smart
Left-Handed Fabric,
Material
Fl
Flow
Control

Disc drives

Solar Panels,
Antennas
Antennas,
Wings,

Deployable Space
Structures

Biological Motivations for


Tensegrity Structures
UCSD 2009 - Cluster No. 7