AICE Biology Guided Reading: Enzymes Ch 3

Answer each of the following questions as you read the assigned chapter(s) in your text. The point value
for each question is found in ( ) at the end of the question. This assignment is worth 20 points total and is
due Thursday, September 11.
1. What is the function of a biological catalyst? (1)
2. What is an enzymes active site? Draw an example of an enzyme-substrate complex, labeling the
enzyme, active site and substrate. (1)
3. Draw graphs to illustrate an endergonic and an exergonic reaction. Be sure to label the reactants,
products and activation energy. Indicate for each reaction whether the products or reactants have
more energy. (4)
4. What is activation energy? Describe how enzymes act to reduce activation energy. (2)
5. Why do organisms or cells only need to have very small quantities of a particular enzyme? (1)
6. Describe the pros and cons of two different methods of measuring reaction rate of the catalasehydrogen peroxide reaction. (2)
7. Looking at the catalase hydrogen peroxide reaction discuss the effects of the following factors
on enzyme activity (3)
a. substrate concentration
b. temperature
c. pH
8. Define competitive inhibition, non competitive inhibition, and activation. As part of your
definition, discuss how each relates to the allosteric site. (3)
9. Looking specifically at non competitive inhibition, differentiate among reversible, irreversible and
end product inhibition (3).