Answer guide for O Level Biology (5090/3) – Paper 3 Practical Test November 1999

1(a)(i) Drawing marks:

3
1. At least 7 cm diameter clear & clean
2. Pattern of bundles & thickening shown
3. Bundle detail from observation with lens
Labels: Vascular bundle ;
pith / hollow ;
epidermis ; thickening ; [up to 3] 3
(ii) Line ruled on drawing & measured mm / 0.1 cm ;
Size of image divided by size of specimen ;
Magnification correctly calculated & well stated ;
[up to 2 decimal points, but no mark for excessive rounding] 3
(b) Drawing marks:

3
1. At least 7 cm clear & clean with 5 ridges inside & outside
2. Labels: Vascular bundle / vein ; xylem / vessel ; epidermis ;
[2 correct = 1, 3 correct = 2] 2
(c)(i) Three from:
Similarities:
Epidermis / outer covering ;
Veins / bundles ;
Central hollow / pith / soft interior ;
Strengthening / thickening layer ; [up to 3] 3

Page 1 of 2 © UCLES 2002

Answer guide for O Level Biology (5090/3) – Paper 3 Practical Test November 1999

(ii) Differences: [Each line comparative / about same feature]

W31 W32
rough – smooth ;
round – angular ;
hard / woody – soft / fleshy ;
many bundles – few(er) bundles ; 4
[Accept other reasonable contrasts]

2(a) Drawing marks:

1. At least 4 cm clear & clean, radicle & plumule shown

2. Testa shown
3. Leaf detail on plumule, from lens 3
Labels: Testa & cotyledon ;
radicle & plumule ; 2
(b)(i) To make a solution of the oil ; 1
(ii) Add drops of water (to filtrate) / pour filtrate into water ; 1
(iii) Observation: goes cloudy ;
Conclusion: oil (fat) present ; 2
(c) Observation: no / less cloudiness ;
Conclusion: little / no oil remains ; 2
(d) To get clear filtrate / solution / to remove ash etc ; 1
(e)(i) From oil ; in cotyledons ; reference to photosynthesis / light ;
water absorbed ; enzymes mobilised ; lipase ; [up to 4] 4
(ii) Energy lost / used in burning ;
emerges as heat ; light ; 3

Required for question 1:

W31 Piece of internode of bamboo
W32 Piece of internode of Cucurbita.

Required for question 2:

W33 A seed of groundnut.

Page 2 of 2 © UCLES 2002