Search ExPASy web site for Go Clear

ExPASy Proteomics Server

Databases Tools Services Mirrors About Contact

You are here: ExPASy CA > Tools > Primary structure analysis > ProtParam

ProtParam
User-provided sequence:
10 20 30 40 50 60
HHHHHDYDIP TTENLYFQGA MDPMTEYKLV VVGAGGVGKS ALTIQLIQNH FVDEYDPTIE

70 80 90 100 110 120

DSYRKQVVID GETCLLDILD TAGQEEYSAM RDQYMRTGEG FLCVFAINNT KSFEDIHQYR

130 140 150 160 170 180

EQIKRVKDSD DVPMVLVGNK CDLAARTVES RQAQDLARSY GIPYIETSAK TRQGVEDAFY

190 200 210

TLVREIRQHK LRKLNPPDES GPGCMSCKCV LS

References and documentation are available.

Please note the modified algorithm for extinction coefficient.

Number of amino acids: 212

Molecular weight: 24056.0

Theoretical pI: 5.24

Amino acid composition: CSV format

Ala (A) 12 5.7%
Arg (R) 12 5.7%
Asn (N) 6 2.8%
Asp (D) 18 8.5%
Cys (C) 6 2.8%
Gln (Q) 12 5.7%
Glu (E) 15 7.1%
Gly (G) 14 6.6%
His (H) 8 3.8%
Ile (I) 12 5.7%
Leu (L) 15 7.1%
Lys (K) 11 5.2%
Met (M) 6 2.8%
Phe (F) 6 2.8%
Pro (P) 8 3.8%
Ser (S) 11 5.2%
Thr (T) 13 6.1%
Trp (W) 0 0.0%
Tyr (Y) 11 5.2%
Val (V) 16 7.5%
Pyl (O) 0 0.0%
Sec (U) 0 0.0%

(B) 0 0.0%
(Z) 0 0.0%
(X) 0 0.0%
Total number of negatively charged residues (Asp + Glu): 33
Total number of positively charged residues (Arg + Lys): 23

Atomic composition:

Carbon C 1049
Hydrogen H 1643
Nitrogen N 293
Oxygen O 332
Sulfur S 12

Formula: C1049 H1643 N293 O332 S12

Total number of atoms: 3329

Extinction coefficients:

This protein does not contain any Trp residues. Experience shows that
this could result in more than 10% error in the computed extinction coefficient.

Extinction coefficients are in units of M -1 cm-1 , at 280 nm measured in water.

Ext. coefficient 16765

Abs 0.1% (=1 g/l) 0.697, assuming all pairs of Cys residues form cystines

Ext. coefficient 16390

Abs 0.1% (=1 g/l) 0.681, assuming all Cys residues are reduced

Estimated half-life:

The N-terminal of the sequence considered is H (His).

The estimated half-life is: 3.5 hours (mammalian reticulocytes, in vitro).

10 min (yeast, in vivo).
>10 hours (Escherichia coli, in vivo).

Instability index:

The instability index (II) is computed to be 40.79

This classifies the protein as unstable.

Aliphatic index: 77.22

Grand average of hydropathicity (GRAVY): -0.512

Swiss Institute of Bioinformatics | Disclaimer | Sitemap | Documentation | Contact Us