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Abstract
INTRODUCTION
Long before the development of methods in verifying active components in plants, the use of
herbal medicine has been a practice among Filipinos. Without knowing exactly the science
behind the claims, people of the rural areas rely mainly on herbolaryos or quack doctors for
remedy. The trend has subsided with the rise of advanced technology, introduction of science-
based medicine, the trust of the patients for their health concerns has shifted to the expertise of
medical doctors.
Today, there is a paradigm shift on the use of alternative medicines, the reason being mainly on
the side effects and high cost of medicines, let alone the development of resistance among
pathogenic organisms. Secondary metabolites, though present in plants and animals in small
amounts and for defense mechanisms, have received a great deal of attention in the medical
world. Many drugs have been discovered through research into the physiological effects of
chemicals found in plants. Some of these drugs are still derived directly from plants (e.g.
digitalin from foxglove, Digitalis) while others are now synthesized (e.g. aspirin inspired by the
medicinal properties of the bark of the willow, Salix alba). Chemicals in some plants are
extracted and transformed, providing the building blocks of drugs (e.g. progesterone,
synthesized from chemicals found in some species of Dioscorea, the wild yam (BGCI Fact
Sheet, 2000).
As a preliminary step, phytochemical screening is geared towards the discovery of the array of
potentials a species holds.
Objectives
The study aimed to perform phytochemical screening of dalanghita, citrus nobilis, peel.
Scope and Delimitation
The study was delimited to the phytochemical screening dalanghita, Citrus nobilis,
peel. Only the peel were used in the experimental investigation. The fruits were gathered
from Brgy. Bangkal, Abucay, Bataan.
The determination of the chemical constituents was limited to the qualitative rather than
the quantitative analysis. Only the presence or absence of the secondary metabolites alkaloids,
quaternary bases or amine oxides, saponins, free fatty acids, cardiac glycosides,
leucoanthocyanins, flavonoids, tannins, fats and anthraquinones were given focused to.
Dalanghita, popularly known as sintores in the province of Bataan, is a small tree. The leaves
are smooth, oblong to broadly lanceolate, 4 to 10 cm long, with short petioles of about 1 cm
long. A flowering plant, its flowers are white, solitary and short-pedicelled. The fruits are
hesperidums, with a loose skin and leathery pericarp, with a sweet pulp that is only fairly juicy of
many varieties, the large ones attain a size of about 10 cm.
It is widely cultivated in the Philippines for commercially fruiting, the fruit bearing period is at its
peak during the months of October to February. The fruit is a good source of vitamin A, B and
C, and Citric acid which is 0.35% of the juice, and the rind is used for flavouring. Studies on the
composition of the rind revealed the presence of 14-19.33% volatile oil, 92% limonene,
methylanthranillic acid, and methyl ester Both seeds and cuttings are used for propagation.
Rind is prepared by gathering the rind ripe fruit which is sun-dried, and can be used as either
whole or cut into thin slices. The seeds are placed in a container, where an amount of water is
added to one fiftieth of the total weight of the seeds. This is stored for a short time and put into a
dry kettle. It is then heated with a weak fire until the materials turn light yellow and smell
fragrant. The material is sundried and crushed before it could be used.
This seed preparation has pain relieving effect. In Malaya, a decoction of the roots is used in
dysentery. Powdered leaves with leaves of Areca catechu may be drunk for stomach ache. An
infusion of the fresh juice is used as a cleanser or stimulant of wound surfaces. A lotion of the
boiled leaves is used hot on painful places and swelling in Malaya. Seed preparation tastes
bitter with pain relieving effect. Rind preparation tastes bitter, with mild nature. The fibers of the
rind tastes bitter-sweet and neutral natured. The rind is also used for nausea and fainting. It is
squeezed near the nostril for instant inhalation. Decoction of roots is used for cough and fever.
For anorexia and vomiting, fresh rhizome of ginger is added to a concentrated decoction of the
rind and this decoction is drunken. Decoction of dried flowers is used for diarrhea.
Decoction of rind or peel used to regulate monthly period.
Leg bath of boiled leaves used for rheumatism, and painful and swollen legs.
The oil from the rind is used for stomach problems; and as liniment for gout, rheumatism and
other painful swellings.
Phytochemical screening of the volatile compounds in three selected Asian citrus fruits (C.
nobilis, C. sinensis, C reticulata) revealed a total of 51 compounds in C. nobilis: terpenes,
carbonyls, alcohols, esters and hydrocarbons, with limonene as the main compound.
Naringin, a prominent bioflavonoids in grapefruit and other citrus fruits, is present in C. nobilis.
Study on the free radical scavenging of naringin revealed a dose-dependent scavenging and
demonstrates it can protect mouse bone marrow cells against radiation-induced chromosomal
damage.
Nobiletin, a flavonoid present in the peel of many citrus fruits, especially prevalent in C nobilis,
in a mixture with ethanol and glycerol, applied to the ears of hamsters, once daily for 14 days,
showed a decrease in the amount of triacylglycerol on the bskin surface, reduced the sizeof and
amount of oil produced by the sebaceous glands. Triacylglycerol is the main constituent of
mammalian fat and a major component of sebum. Results suggest a potential in the treatment
of acne.
Alkaloids. These are chemical substances which are nitrogen heterocycles which occur mainly
in plants as their salts of common carboxylic acids. They constitute an indispensable and most
potent group of substances for the treatment and mitigation of functional disturbances and relief
from suffering. Alkaloids are anti-hypertensive, antineoplastic agents and demonstrate anti-
tumor activity.
Cardiac glycosides. A chemical group of glycosides used to treat heart failure and irregular
heart beat . An example is digitoxin.
Extract. This refers to the solution obtained from the leaves of mandarin orange peel.
Flavonoids. A naturally occurring phenolic compound belonging to a large group that includes
many plant pigments. Flavonoids have beneficial effects in the human diet as antioxidants,
neutralizing free radicals which damage body tissues and leads to heart disease, stroke and
cancer.
Reducing sugar. A reducing sugar is any sugar that, in a solution, has an aldehyde or a ketone
group.
Saponins. These are glycosides with distinctive foaming characteristics. They consists of a
polycyclic glycone that is either a choline steroid or triterpenoid. Attached via C3 and ether
bound to a sugar side-chain.
Tannins. They are substances which occur as mixture of polyphenols which are very difficult to
separate since they do not crystallize.
Terpenoids. The terpenoids, sometimes called isoprenoids, are a large and diverse class of
naturally-occurring organic chemicals similar to terpenes, derived from five-carbon isoprene
units assembled and modified in thousands of ways. Most are multicyclic structures that differ
from one another not only in functional groups but also in their basic carbon skeletons. These
lipids can be found in all classes of living things, and are the largest group of natural products.
Plant terpenoids are used extensively for their aromatic qualities. They play a role in traditional
herbal remedies and are under investigation for antibacterial, antineoplastic, and other
pharmaceutical functions.
Methodology
This section presents the design of the study, materials and experimental procedures
and the statistical treatment of data.
Procedures:
The fruits of C. Nobilis were peeled off, the peel blanched with denatured alcohol to stop any
enzymatic activity that will make the peels rot, then the peels were air dried for a week. After air
drying, the plant samples were turned into uniform powder using a blender, and then weighed.
Eighty-five grams of the powder was obtained, after which 850 mL denatured alcohol was
added to this sample to extract the secondary metabolites present. This set-up was filtered after
48 hours using Whattman No. 1. The extracts were concentrated using a water bath.
Phytochemical screening
Results on the phytochemical screening of the Citrus nobilis peel are presented in the
following table.
Reducing sugars
Reducing sugars are present in the peel, as observed in the Fehling’s test.
Anthraquinones
Anthraquinones were found to be absent in the peel of dalanghita.
Terpenoids.
Terpenoids were present in the peel. Terpenoids are under investigation for
antibacterial, antineoplastic, and other pharmaceutical functions.
Flavonoids
Flavonoids can also be found in the peel of dalanghita as evidenced by the formation of
a red color using Wilstatter test.
Flavonoids have antiviral, anti-inflammatory and cytotoxic properties (Capal, 1992)
Saponins
The peel of C. nobilis do not contain saponins. On Froth test, the peel extract did not
form froth that persisted for 30 minutes.
Tannins
Using Ferric chloride test, the presence of tannins was detected by the formation of a
brownish-green precipitate.
Tannins are possible sources of chemicals for the treatment of diarrhea and extensive
burns and maybe used rectally for the relief of various rectal disorders (Santos, 1985). It is also
used n the treatment of bed sore and weeping ulcers. It was formerly used for sore throat and
stomatitis.
Alkaloids. As gleaned from the summary of results. Dalanghita peel yielded positive result fo
alkaloids using Dragendorff’s test. This is evidenced by the formation of reddish brown
precipitate.
Alkaloids are widely used in medicines like morphine, codeine, etc. Alkaloids are anti-
hypertensive, antineoplastic agents and demonstrate encolytic property. It is used to relieve
nasal congestion, stop hemorrhage, combat malaria and dilate the pupil of the eye and also
used as a muscle stimulant. (The US Educator Encyclopedia, 1984).
Cardiac Glycosides
The peel extract of C. nobilis contains cardiac glycosides. This was evidenced by the
formation of a reddish brown color at the interface using the Keller –Kiliani Test.
Cardiac glycosides have effects on the heart and kidneys and affect the contractions of
the heart muscles.
Conclusions
The dalanghita (Citrus nobilis) peel extracts contain reducing sugars, terpenoids,
alkaloids, cardiac glycosides, flavonoids and tannins. These substances can become excellent
sources of therapeutic substances and as additive to cosmetic products.
Recommendations
1. A follow up study should be conducted to quantify, isolate and identify the type of
reducing sugars, terpenoids, alkaloids, cardiac glycosides, flavonoids and tannins
present in the peel of dalanghita.
2. Tests on the presence of other compounds are highly recommended, to further
explore the potential of dalanghita peel as a source of alternative drug and other
industrial applications.
3. Pharmacological testing should be done.
4. Other parts of dalanghita could be used for phytochemical screening and bioassays.
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Mandarin orange (Engl.)
C. madurrensis
C. nobilis Naranjita (Span.)
C. papillaris Ransas (Bik.)
C. reticulata Sintonis (Tag.)
C. webberi Sinturis (Tag.)
Tison (Tag.)
Tangerine orange (Engl.)
Tangor (Engl.)
Ch'en P'i (Chin.)