Table 1.

Effect of different levels of Cd on lymphocyte proliferation

in vitro
Day of blood sample (Day of calving) for lymphocyte culture
Cd level
Mean
-30 -15 0 15 30
± SE
1.231 0.972 1.009 1.017 1.065 1.058a
10ˉ6M
± 0.025 ± 0.038 ±0.023 ± 0.018 ± 0.033 ± 0.045
1.002 0.966 1.003 0.952 1.010 0.986b
10ˉ5M
± 0.069 ± 0.035 ± 0.015 ± 0.026 ± 0.008 ± 0.011
0.996 0.860 0.893 0.929 0.962 0.928c
10ˉ4M
± 0.037 ± 0.031 ± 0.006 ± 0.032 ± 0.033 ± 0.024
0.783 0.760 0.865 0.922 0.930 0.852d
10ˉ3M
± 0.066 ± 0.064 ±0.034 ± 0.026 ± 0.030 ± 0.034
Mean 1.003 0.931 0.942 0.955 0.991
± SE ±0.091 ±0.030 ±0.037 ±0.021 ± 0.029

Mean bearing different superscripts differ significantly (P<0.05

Table 2. Effect of different levels of Zn on Cd treated lymphocytes on their proliferation
in vitro
Day of
blood Cd level 50 µm Zn 55 µm Zn 60 µm Zn
sample
10ˉ6 M 1.403 ± 0.294 1.808± 0.331 1.081 ± 0.004

10ˉ5 M 1.135 ± 0.033 1.227± 0.063 1.240 ± 0.033

-30
10ˉ4 M 1.053 ± 0.041 1.079 ± 0.090 1.105 ± 0.054

10ˉ3 M 0.839 ± 0.035 0.977 ± 0.038 1.081 ± 0.004

10ˉ6 M 1.201 ± 0.002 1.509 ± 0.017 1.704± 0.059

10ˉ5 M 1.123 ± 0.061 1.188 ± 0.025 1.166 ± 0.077

-15
10ˉ4 M 1.014 ± 0.006 1.014± 0.004 1.075 ± 0.087

10ˉ3 M 0.817 ± 0.047 0.951 ± 0.028 0.990 ± 0.055

10ˉ6 M 1.14 ± 0.014 1.204 ±0.005 1.14 ± 0.022

10ˉ5 M 1.080 ± 0.005 1.103 ±0.042 1.107 ±0.054

0
10ˉ4 M 0.96 ± 0.039 1.024± 0.057 1.066± 0.034

10ˉ3 M 0.808 ± 0.031 0.920 ±0.019 0.949± 0.039

10ˉ6 M 1.211± 0.057 1.321 ± 0.031 1.751 ± 0.023

10ˉ5 M 1.12 ± 0.049 1.154± 0.042 1.201 ± 0.005

15
10ˉ4 M 1.021 ± 0.005 1.040 ±0.025 1.085 ±0.038

10ˉ3 M 0.821 ± 0.012 0.969 ± 0.040 1.002 ± 0.056

10ˉ6 M 2.041± 0.056 2.202 ± 0.037 2.503 ± 0.098

10ˉ5 M 1.699 ±0.018 1.902 ± 0.035 2.204 ± 0.053

30
10ˉ4 M 1.401 ± 0.093 1.503 ± 0.074 1.601 ± 0.051

10ˉ3 M 1.011 ±0.030 1.303 ± 0.081 1.401 ± 0.023

Mean ±SE 1.145a ± 0.152 1.270b ± 0.175 1.364c ± 0.219

Mean bearing different superscripts differ significantly (P<0.05)