Академический Документы
Профессиональный Документы
Культура Документы
Materials:
Procedure
About 100 ml of this solution is adequate for one large eye or several small
eyes. The eye should be placed in the solution immediately after enucleation
and trimming. A gauze pad should be used to keep the eye submerged if
necessary or inconsistent fixation will occur. The globe should remain in the
solution for at least three hours. The eye then is taken out of the fixative and
washed. Although 50% ethanol is the recommended agent for washing, you
can fill the container with tap water and decant and replace the water every
30-60 minutes throughout the day; this is not ideal, but is adequate for
routine histology. The eye then is stored in 70% ethanol and sent to the
laboratory.
Be sure to dispose of the original Bouin's solution and the decanted water
appropriately.
Procedure
About 100 ml of this solution is adequate for one large eye or several small
eyes. The eye should be placed in the solution immediately after enucleation
and trimming. A gauze pad should be used to keep the eye submerged if
necessary or inconsistent fixation will occur. The globe should remain in the
solution for one to two days. The eye then is taken out of the solution and
placed either in formalin or 70% ethanol. To make the eye easier to cut, you
could leave it in 70% ethanol for a day, transfer to 90% for a day and finally
to 100% for a day.
Abstract
For histopathological examination of the eye, apposition and preservation of rods and
cones, and nuclear layers of the retina were slightly inferior with mDF compared to DF.
This paper has demonstrated that mDF provides comparable, and in many respects
superior preservation of the testes to that of BF, both for IHC staining and for detailed
histopathological examination. It also provides an acceptable fixative for eyes, although
the quality of cellular preservation is inferior to that of DF.