Raw Data Table 1

Raw Data Table I Crude Extract 40% Supernatant 60% Pellet

Total volume* (ml) 40.5 43 5

Duplicates #1 #2 #1 #2 #1 #2
ΔA/Δt 0.144 0.142 0.114 0.116 0.121 0.13
LDH Enzyme Assay

Conc. In dilution assayed

0.6945338 0.6848875 0.5498392 0.5594855 0.5836013 0.6270096
(units/ml)
Dilution factor 200 200 200 200 560 560
Undiluted conc. (units/ml) 138.90675 136.97749 109.96785 111.89711 326.81672 351.1254
total amount (units) 5625.7235 5547.5884 4728.6174 4811.5756 1634.0836 1755.627
average (units) 5586.65594855305 4770.09646302251 1694.85530546624
±Δ (units) 39.0675241157551 41.4790996784568 60.7717041800645
%Δ 0.69930069930069 0.869565217391309 3.58565737051794
A595 0.6669 0.6708 0.5679 0.538 0.8861
μg/50 μl (from standard curve) 8.6052 8.6635 7.1503 6.7251 11.106
Protein Assay

Dilution factor 25 25 25 25 35
Undiluted conc. (mg/ml) 4.3026 4.33175 3.57515 3.36255 7.7742
total amount (mg) 174.2553 175.43588 153.73145 144.58965 38.871
average (mg) 174.8455875 149.16055 38.871
±Δ 0.590287499999988 4.57089999999999
%Δ 0.337605031067763 3.06441616097554
 Raw Data Table II

Raw Data Table II

Total Protein Total Activity Specific Activity Yield Purification Factor
Total Total Specific ±Δ Purif.
±Δ Sum* of ±Δ** Yield Sum of Sum of ±Δ
Protein %Δ ±Δ (mg) Activity %Δ
(units)
Activity
%Δ (units/mg) (%) *** %Δ
(yield Factor
%Δ (fold)
(mg) (units) (units/mg) %) (fold)

Crude
174.846 0.33761 0.59029 5586.656 0.699301 39.06752 31.951941 1.03691 0.331312 100 - - 1 - -
Extract
40%
Super- 149.161 3.06442 4.5709 4770.096 0.869565 41.4791 31.979612 3.93398 1.258072 85.3838 1.56887 1.33956 1.00087 4.97089 0.04975
natant
60%
38.871 - - 1694.855 3.585657 60.7717 43.602051 4.28496 1.86833 60.6751 4.28496 2.5999 1.36461 5.32186 0.07262
Pellet

Affinity
1.79726 1.46241 0.02628 796.4108 2.693455 21.45096 443.12462 3.39276 15.03414 28.5112 3.39276 0.96731 13.8685 4.42966 0.61433
Column
*sum the %Δ's from components of the calculation **Calculate the actual Δ from the %Δ ***The yield was doubled in the 60% pellet and the
affinity column because only 1 pellet was used
 Table 1: Purification Table
Specific
Total Protein Total Activity Purif. Factor
Activity Yield (%)
(mg) (units) (overall fold)
(units/mg)
Crude
174.9 ± 0.6 5590 ± 40 31.9 ± 0.3 100 1
Extract
40%
150 ± 5 4470 ± 40 32 ± 1 85 ± 1 1.00 ± 0.05
Supernatant
60% Pellet 38.9 1690 ± 60 44 ± 2 61 ± 3 1.4 ± 0.1
Affinity
1.80 ± 0.05 800 ± 20 450 ± 20 29 ± 1 13.9 ± 0.6
Column
*

er
Sample Protein concentration Enzyme concentration (units/ml)
60% 7.7742 338.971061093247
1 1.43496 0
2 5.52468 0
3 4.546675 0
4 1.381075 0
5 0.01019 0
6 0.007472 0
7 1.15257 553.697749196141
8 0.4058875 139.389067524115
9 0.081225 8.97106109324759
10 0 0
11 0 0

Elution Profile
9 600

8
500
7

Enzyme concentration (units/ml)

Priotein Concentration (mg/ml)

6 400

5
300
4

3 200

2
100
1

0 0
60% 100% 200% 300% 400% 500% 600% 700% 800% 900% 1000% 1100%
 0
00%
100
200
300
400
500
600
Enzyme concentration (units/ml)