ENZYME Product Source Isoschizo- Conditions for

&Recogni mers double

-tion Site (if any) digestion
EcoRI 5’ sticky ends An E. coli strain that - Digest in NEBuffer
G’AATTC carries the cloned EcoRI + BSA at
EcoRI gene from E. 37°C.
coli RY13
BamHI 5’ sticky ends A E. coli strain that -
G’GATCC carries the BamHI gene
from Bacillus
amyloliquefaciens
BglII 5’ sticky ends A E. coli strain that - Digest in NEBuffer
A’GATCT carries the BglII gene 3 + BSA at 37°C.
from Bacillus globigii
PvuI 3’ sticky ends An E. coli strain that BpvUI, MvrI,
CGATC’G carries the cloned PvuI Ple19I
gene from Proteus
vulgari
PvuII Blunt ends A E. coli strain that - Digest in
CAG’CTG carries the PvuII gene NEBuffer 2 at
from Proteus vulgaris 37°C.
Mbo I 5’ sticky ends A E. coli strain that BfuCI,
G’ATC carries the MboI gene Bsp143I,Bss
from Moraxella bovis MI, BstKTI^, OR
DpnII, Kzo9I,
NdeII, Digest in
Sau3AI NEBuffer 4 at
37°C.

HindIII 5’ sticky ends A E. coli strain that - Digest in NEBuffer

A’AGCTT carries the HindIII 2 at 37°C
gene from
Haemophilus
influenzae
HinfI 5’ sticky ends A E. coli strain that -
G’ANTC carries the HinfI gene
from Haemophilus
influenzae
Sau3AI 5’ sticky ends An E. coli strain that BfuCI, Digest in
G’ATC carries the cloned Bsp143I, NEBuffer 1 +
Sau3Al gene from BstKTI^, BSA at 37°C.
Staphylococcus aureus BstMBI,
3A DpnII, Kzo9I,
MboI, NdeII OR
AluI Blunt ends An E. coli strain that AluBI
AG’CT carries the cloned AluI Digest in
gene from NEBuffer 4 +
Arthrobacter luteus BSA at 37°C.

TaqI 5’ sticky ends An E. coli strain that - Digest in

T’CGA carries a TaqαI NEBuffer 4 +
overproducing plasmid BSA at 37°C with
HaeIII 5’ sticky ends A E. coli strain that BshFI, BsnI, HaeIII, then add
G’GCC carries the HaeIII gene BspANI, TaqαI and raise
from Haemophilus BsuRI, PhoI temperature to
aegypticus 65°C.