UDBB2234 Laboratory2B.

khocl

UNIVERSITI TUNKU ABDUL RAHMAN

Name:
ID No:
Group Members:
Laboratory Report Sheet (Exp 1)

Title:

Objective:

Results/Observations:

What is the concentration of the plasmid DNA (pBR322) used in this

experiment (in ng/µl)?

Attach a print out of the gel image. Calculate the amount of DNA loaded
in each lane.

Analysis and Conclusions:

1. Based on the result obtained, what is the detection sensitivity of agarose gel
electrophoresis?

2. How many bands can you observe from the lane that is loaded with the highest
amount of DNA? Explain what are those bands represent and how they differ
from each other.

Questions
1. Explain the functions of loading dye and ethidium bromide used in the
experiment.

2. From a small culture, you have purified the DNA of a recombinant plasmid. You
have resuspended the DNA in a volume of 50 µl. You dilute 20 µl of the purified
DNA sample into a total volume of 1000 µl distilled water. You measure the
absorbance of this diluted sample at 260 nm and 280 nm and obtain the following
readings.
A260 = 0.550
A280 = 0.324

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UDBB2234 Laboratory2B. khocl

(a) What is the concentration of the 50 µl plasmid prep?

(b) How much total DNA was purified by the plasmid prep procedure?

(c) Determine the purity of the purified DNA.

3. Forty microliters of a stock solution of RNA is diluted with water to give a final
volume of 1000 µl. The diluted sample has an absorbance at 260 nm of
0.142. What is the concentration of the RNA stock solution in µg/ml?