Con. 2360-11. (3 Hours) N.B,: (1) All questions are compulsory. (2) All questions carry equal marks Q.1 A) Explain various methods of protein separation and purification. B) Write an account on disc electrophoresis of OR ‘A) Elaborate on any two techniques of separation by centrifugation B) List the key goals of Human Genome Project. LR-9230 [Total Marks : 75 3) @M ® a Q.2 A) What is MALDI? Discuss various compounds used as matrix in MALDI end its advantages. (8) B) What is Brage’s law and give derivation for Brage’s law of X-ray diffraction. ) - OR ~ A) Explain the following term ; @) i) FRET ii) SIRNA B) Discuss Yeast Two hybrid system with the help of neat diagram and its significance o Q.3 A) Give applications of UV-visible spectroscopy in biological sample analysis, ”) B) What is proteomics? How mass spectrometry and bioinformatics help in proteomic studies? (8) OR A) What is FTIR? Draw a schematic diagram of FTIR instrumentation and indicate all parts 8) B) Discuss Gene Libraries and their applications 0) Q-4 A) What are post translational modifications? Elaborate on its significance with examples. @) ~ 8) Justify the statement — “There are different proteins made by the same total number of amino acids” ® OR A) Discuss the designing of a microarray experiment w.r.to DNA microarray _ (10) B) Describe the unusual rules employed by mitochondrial tRNAs to decode mitochondrial genetic message. (6) Q.5 Write short notes on following (any three) (1s) A) Alternative splicing mode! B) Mitochondrial genome ©) reduced genomes w.r.to E.coli 2) Affinity chromatography B) in situ hybridisation .