GEN 3040 DNA Sequencing

GEN 3040
Introduction The fundamental of the genome project is to complete the DNA sequence for the organism being studied and include together with the genetic and physical maps of the genome so that genes and other interesting features can be located within the DNA sequence. Therefore, the techniques and research strategies for the DNA sequencing had been explored by many researchers. Sequencing is the process to determine the exact order of the nucleotides in a region of DNA. A combination of the computer analysis and experimentation are required to find the location of the gens and their control regions (Brown, 2006). DNA sequencing methods include the chain termination method introduced by F.Sanger and A.R. Coulson and chemical degradation by A.Maxam and W.Gilbert. Both of these techniques are important in allowing DNA sequences to be determined in just a short time. For the chain termination method, the sequencing involved the enzymatic synthesis of the strand that complement to an existing template. Thus the DNA to be sequenced must be in single stranded form and the region to be sequenced must be in the 3 flanked by known sequence (Sanger, 1981). The requirements for the Sanger-Coulson sequencing include a primer that complement to the known region to direct the synthesis of the chain, DNA polymerase, 4-deoxynucleotide triphosphates (dNTPs) and 4-dideoxynucleotide triphosphates (ddNTPs) (Brown, 2006). Primer is the single stranded oligonucleotides and since it complement with the DNA, the primer would easily hybridize with the DNA (Sanger, 1981). The DNA polymerization caused the 3-OH to change to a H in ddNTPs, it is unable to form a phosphodiester bond by nucleophilic attack on the phosphate and it would cause a termination in the DNA chain. There are two forms of labeling, the radioactive form that include the labeled primer with 32P or 33P and labeled dNTP 35S while for the non-radioactive form the primer labeled and ddNTP labeled (big dye terminator). As a result, labeled chains with different size that could be separated by gel electrophoresis before visualized the fragments by using autoradiograph (Brown, 2006). Different from Sanger-Coulson method, the automatic DNA sequencing though also carried out using the chain termination procedure, the automatic DNA sequencing involves dyelabeled deoxy terminators in which a dye label is inserted into the DNA as well as the terminating base. This method is much safer compared to the Sanger-Coulson since no radioactive involved and the automated sequencers could read up to the 96 sequences thus the data is acquired more rapid the manual sequencing (Brown, 2006).
Plasmid isolation, automated DNA sequencing and bioinformatics analysis
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The computer analysis and internet databases enable the DNA to be analyzed and studied. One popular database is from NCBI in which contain the general database that is very useful. The program BLAST in NCBI could be used to find the homology gene that is similar to the gene being studied. The searching of the homology gene involves the whole collection of sequences in the database. Thus, the sequence could be compared and the origin and functions can be investigated.The nucleotides sequence also could be translated to cDNA, mRNA and amino acid sequence easily by using computer programs. Only short time required and the result obtained also more accurate. With these entire databases on the internet, people also could share their findings easily with other people around the world (NCBI, 2010). As for molecular cloning, the process begins with the isolation and amplification of the gene of interest. The gene of interest is then inserted into a vector, another DNA molecule that will drive the gene into living cells to be replicated. Recombinant DNA will be ultimately produced to be inserted into the host cell. Clones will then can be generated and the inserted foreign DNA will also be generated. The amplified foreign DNA can be purified for further analysis. In this study, the DNA that has been subcloned into a plasmid from a genomic clone promoter fragment (pOS2) of the osmotin gene from Nicotiana tabacum was prepared and sequenced. The desired DNA fragment was purified before sent for the sequencing. The basic of the automated DNA sequence analysis was introduced during the practical. After the sequences were obtained, the sequences were analyzed and mapping by using those database online. By analyzing the sequences, a lot of information of the sequences was obtained. The sequences are obtained from the Nicotiana tabacum gene. The sequence motifs that act as the transcription factor binding sites are also determined.
Materials and methods The DNA has been subcloned into a plasmid from a genomic clone promoter fragment (pOS2) of the osmotin gene from Nicotiana tabacum. The plasmid DNA then was sent for the
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automated sequencing. Then the quantification of the nucleic acids by using Nanodrop and UV spectrophotometry were done. The absorbance at certain wavelength was recorded and the DNA concentration was calculated. All the steps and methods in this experiment are based on the lab manual. The vector used was pUC118 and the restriction enzymes used were pUC118, Xbal, SubcloneSalI, SubcloneSpeI, SubcloneBglII, SubcloneXbal and SpeI, SubcloneSalI and BglII and SubcloneXbal and BglII. The sequence obtained (1st_Base_308861_POS_2_M13F_-20) and (1st_Base_308862_POS_2_M13R_-20) were first edited manually by using the BioEdit Software. Later, the edited consensus sequence that had been reversed was analyzed in the BLAST from NCBI. The homology gene of those sequences was obtained. ClustalW was used to find the genetic similarities between five homology genes. The sequences also analyzed at the Expasy-Translate to convert it into amino acid sequence. The sequence motifs were identified by using the Patmatmotif and also the Fuzznuc.
Results Week 1: Preparation of Plasmid DNA Template for Automated Sequencing
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Initially, only sample A was observed to show pink pellet. However, as the experiment was continued, the pellet appeared in both sample A and B. Thus, both samples were used for next experiment.
Week 2: Quantification of Nucleic Acids Using UV Spectrophotometry Table 1. The OD reading of of sample A and B at 230nm, 260nm and 280nm and the OD ratio by using Nanodrop OD reading 230nm 260nm 280nm Ratio of OD230:OD260:OD280 Sample A Sample B 0.2537 0.2517 0.6162 0.5734 0.327 0.2959 1: 2.4:1.3 1:2.3:1.2
Concentration obtained from the Nanodrop: Sample A: (624.5 + 607.9)/2 = 616.2ng/L Sample B: (563.8 + 583)/2 = 573.4 ng/L
Table 2. The OD reading of two different concentration of sample A at 230nm, 260nm and 280nm and the OD ratio (UV spectrophotometer) OD reading 230nm 260nm 280nm
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999L H2O + 1L DNA 998L H2O + 2L DNA
0.0011 -2.2157
-0.0140 -1.9648
-0.0217 -2.1683
Table 3. The OD reading of two different concentration of sample B at 230nm, 260nm and 280nm and the OD ratio (UV spectrophotometer) OD reading 230nm 999L H2O + 1L DNA 998L H2O + 2L DNA 0.0018 -0.0826 260nm -0.0110 1.133 280nm -0.0198 1.023
The concentration of the samples can be calculated by using the formula given as follows: Concentration of nucleic acid = absorbance at OD260 x 50g/mL x dilution factor Table 4. The distance migrated, size of markers, and log of length of markers Distance migrated (cm) 0.9 1.3 1.6 2.1 3.2 3.3 5.2 6.5 Size of markers 23130 9416 6557 4361 2322 2027 564 125 Log of length (bp) 4.3641756 3.9738664 3.8167052 3.6395861 3.3658622 3.3068537 2.7512791 2.0969100
Figure 1. Graph of distance migrated (cm) versus log of marker (bp) of markers From the graph, the equation obtained is: y = -2.7159x + 12.286
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Thus, to calculate the length of the markers, x = (y 12.286)/-2.7159 Length of restriction enzyme = 10x
Table 5. Distance migrated (cm) and length of restriction enzyme (cm) Restriction enzyme pUC118, Xbal Distance migrated (cm) 2.6 Log of length (bp) x = (2.6 12.286)/-2.7159 = 3.566 SubcloneSalI 2.6, 4.2 x = (2.6 12.286)/-2.7159 = 3.566 x = (4.2 12.286)/-2.7159 = 2.977 SubcloneSpeI 2.1 x = (2.1 12.286)/-2.7159 = 3.751 SubcloneBglII 2.5, 4.4 x = (2.5 12.286)/-2.7159 = 3.603 x = (4.4 12.286)/-2.7159 = 2.904 SubcloneXbal and SpeI 2.2, 6.3 x = (2.2 12.286)/-2.7159 = 3.714 5176.07 801.68 4008.67 5636.38 948.42 3681.29 Length (bp) 3681.29
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x = (6.3 12.286)/-2.7159 = 2.204 SubcloneSalI and BglII 2.6, 4.4, 6.6, 6.8 x = (2.6 12.286)/-2.7159 = 3.566 x = (4.4 12.286)/-2.7159 = 2.904 x = (6.6 12.286)/-2.7159 = 2.094 x = (6.8 12.286)/-2.7159 = 2.020 SubcloneXbal and BglII 2.6, 4.4, 6.6 x = (2.6 12.286)/-2.7159 = 3.566 x = (4.4 12.286)/-2.7159 = 2.904 x = (6.6 12.286)/-2.7159 = 2.094
159.96
3681.29
801.68
124.17
104.71
3681.29
801.68
124.17
Figure 3. The restriction map of the cloned insert The consensus sequence of pOS2
>pOS2
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CGCGCGAGCGCCAACGGTGTACGGGCAGTTGTTTCGGACCTCGATAGTTGCAGCATAAGTACAAGT CACAAAGGCAAGGAGGAAGAAAACAAAAGAAGATCTCAAGTATTCCATGTTTGTGGACATTTTCTTTT TAACAAGTTGCGTTGTTGGATATAGTGACAAATTATTTGGTGGTTTATATAGGAGTATGGCGGCTTTT CGCACATAGACATTAATCATATATATATTTATTAACATTTGACTAGTAAATGGATAATTCTCAAGGATG CTTTAGTAAAGGTGGAGGCGGCTAAAAGTATAAGAGCCGCCTAATAAACTAATAATTTTATGAATATA GAAGTCAATTTATTTGGTGATGATGCTGACCTATAAGCTCTGTAACGTGACACTTGTAACAATATCTCT TTTAGTCAATTTCTTGCTTTCTTTTATCTATACCATTTTTTTAATTCACAAGAAATTGGCAGATAGCCAT CATTTTGAAGCTATTCAATATTAGTTGCTTTTTAACATGAAAATAAACTTAAACAAAAATATTTTTTCGC AAAAATAAGAAAAATATATAAAATTTTTATTAGAGTTTAAATATTTATCAGTTTGAACTTCATATTTCAT ACTCCCGAAAATTTTTATGGAGTTTGCACAGATCATAAATTTTTCATGTAGTTTTAATACTAAATCTTG GAGTTTCACTTTTAATATTGATGTTATATTGTCCCCTACTTATTTTTTCTTCCTTCGTTTTCTTTTTGAGA ACTATTTCATCTATTTATATATAGCAGCTCATTAAGTAGATGGCGTTATTCTGGATGTGGAATAAACAG AGAAGAACAACACAGAAGATTAATGCCAATCGGGTAGACTTGATCAACAATTTAACAAATAAGCTGAT TGTAGATAACATAAACTCATAAAGTTACGCCAATTCAATAGAGTTTGTTGAACAATTGAACTAGCATAA AATCATGCCAACTGGATAGAGTTTGTGAACACTTAACAATATAGGTGGAGTAAATACAATATAAATTC TTCCGATAGAAAAATTTCTGAAGAACTACATGGGTCCGGAGATCATTTTTTAAATATATTATAAAATGG AGACAATATTTAAAGACCAGTCCGTTATAGGCCGGCCATTAATTCTTGTCCATCAAATTTGCATCTGC TGTTGGTTTTCTGCTAGATCTACTTAAAATTTATAAAACAATCAGTTTCGAAACAAAGTCTAGTGTAGT ACTACCAGAACATCAACTCATATAAAAGTCGACTCTAGAGGTCCCCGGTACCGAGCTCGAATTCGA
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Figure 2. The gel electrophoresis of the digestion products
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Figure 4. The pOS2 consensus similarities Week 4: Collation of Sequence Data and Computer Analysis
Figure 5. The edited sequences from BioEdit
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Figure 6. The protein translation of the sequence by using BLAST program
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Figure 7. The Clustalw program to obtain the genetic relationship of the sequences
Figure 8. The Compute pI/Mw tool from Expasy
Discussion During the plasmid preparation, the pink pellet only was observed in the sample A. However, as the experiment was continued, the pink pellet was ultimately observed. Errors might occur during the experiment such as the DNA might be too small or the dye was not completely bind to the plasmid. The concentration of the nucleic acid was obtained directly from Nanodrop as the values obtained from the UV spectrophotometer were erroneous which it gave negative optical density readings. Thus, the values cannot be used by using the OD from the UV spectrophotometer. It might be caused by the spectrophotometer itself, or by the sample as the ratio calculated from the Nanodrop showed that the samples were impure. The DNA concentration was calculated based on the concentration of a double stranded DNA of 50g/mL which is equivalent to an OD260 of 1. The OD of 230 as well as 280nm was also recorded for the precaution purposes. Contamination of the samples by carbohydrates, protein or other foreign components of the tissues will affect the OD260 readings. Thus errors
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when calculating the DNA concentration in the samples could happen. Basically, ratio of OD230:OD260:OD280 of pure DNA sample is 1:1.8 and the accepted range of DNA sample for further analysis is 1:1.6-2:1. However, the DNA sample obtained in this experiment for both sample A and B were 1: 2.4:1.3 and 1:2.3:1.2 respectively. The ratios explained that the DNA samples were not pure and rejected for further analysis. The lacks of DNA purity probably due to the contamination during the DNA transfer from one container to others or poor centrifugation process which caused some debris left in the supernatant. The contamination of the samples was the reason why the OD readings were affected (Brown, 2006). The DNA was cut by pUC118, Xbal, SubcloneSalI, SubcloneSpeI, SubcloneBglII, SubcloneXbal and SpeI, SubcloneSalI and BglII and SubcloneXbal and BglII restriction enzymes and separated based on the sizes by the gel electrophoresis. The lowest band on the gel was the shorter fragment as it could travel further. From the gel of digestion fragments, the length and size of the markers were measured. A graph of distance migrated by the markers versus log of length of the restriction enzyme was plotted and the equation obtained was used in order to calculate the length of the restriction enzymes. From Figure 3, the restriction map of the cloned insert is shown. The exact location of the insert as well as the restriction enzymes can be estimated. The similarities of the consensus can be determined by using the online programs such as BLAST in NCBI that contains the GenBank databases. The highest similarity obtained was 99%. The 1% unlikeliness might be due to some of the degeneration of short fragment caused by the plasmid cutting process by restriction enzymes. When the BioEdit program was ran, the Restriction Map was performed as well. There were 5 restriction enzymes detected with specific bases shown as follows: GAATTC = EcoRI (1) GTCGAC = AccI/HincII/SalI (1) TCTAGA = XbaI GAGCTC = SacI GGTACC = KpnI
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Questions and answers Q1. Do all regions of your sequence show exact homology to the best matching sequence you have found in the database? Not all regions in the sequence showed exact homology of the best matching sequence. The best homology of the pOS sequence is the N.tabacum osmotin gene which shows 99% identical and has 0 E value. The 1% that is incompatible with the cloned sequence probably due to the impurities of the DNA that was sent for sequencing. There might be errors occurred during the isolation process. Q2. Using the BLAST output description, matcher analyses, and the references cited, determine the genetic relationship between the five most closely related DNA sequence and the genomic sequence pf this gene been determined? From BLAST, five most closely related DNA sequences of the consensus sequences pOS2 are the N.tabacum osmotin gene, abscisic acid-activated of N.tabacum, promoter osmotin of N.tabacum, N.tabacum ap24 gene asn Solanum phureja osmotin-like protein. The osmotin gene is the most identical to the sequence with the 0 E-value that indicates the most significant results.
Figure 9. The pOS2 consensus similarities

>gi|1167853|emb|X95308.1| N.tabacum osmotin gene GTCGACTTTTATATGAGTTGATGTTCTGGTAGTACTACACTAGACTTTGTTTCGAAACTGATTGTTTTAT AAATTTTAAGTAGATCTAGCAGAAAACCAACAGCAGATGCAAATTTGATGGACAAGAATTAATGGCCGGC CTATAACGGACTGGTCTTTAAATATTGTCTCCATTTTATAATATATTTAAAAAATGATCTCCGGACCCAT GTAGTTCTTCAGAAATTTTTCTATCGGAAGAATTTATATTGTATTTACTCCACCTATATTGTTAAGTGTT CACAAACTCTATCCAGTTGGCATGATTTTATGCTAGTTCAATTGTTCAACAAACTCTATTGAATTGGCGT AACTTTATGAGTTTATGTTATCTACAATCAGCTTATTTGTTAAATTGTTGATCAAGTCTACCCGATTGGC ATTAATCTTCTGTGTTGTTCTTCTCTGTTTCTTCCACATCCAGAATAACGCCATCTACTTAATGAGCTGC TATATATAAATAGCTGAAATGATTCTCAAAAAGAAAACGAAGGAAGAAAAAATAAGTAGGGGACAATATA
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ACATCAATATTAAAAGTGAAACTCCAAGATTTAGTATTAAAACTACATGAAAAATTTATGATCTGTGCAA ACTCCATAAAAATTTTCGGGAGTATGAAATATGAAGTTCAAACTGATAAATATTTAAACTCTAATAAAAA TTTTATATATTTTTCTTATTTTTGCGAAAAATATTTTTGTTTAAGTTTATTTTCATGTTAAAAAGCAACT AATATTGAATATGCTTCAAAATGATGGCTATCTGCCAATTTCTTGTGAATTAAAAAAATGGTATAGATAA AAGAAAGCAAGAAATTGACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGCTTATAGGTCAGCAT CATCACCAAATAAATTGACTTCTATATTCATAAAATTATTAGTTTATTAGGCGGCTCTTATACTTTTAGC CGCCTCCACCTTTACTAAAGCATCCTTGAGAATTATCCATTTACTAGTCAAATGTTAATAAATATATATA TGATTAATGTCTATGTGCGAAAAGCCGCCATACTCCTATATAAACCACAAATAATTTGTCACTATATCCA ACAACGCAACTTGTTAAAAAGAAAATGTCCACAAACATGGAATACTTGAGATCTTCTTTTGTTTTCTTCC TCCTTGCCTTTGTGACTTGTACTTATGCTGCAACTATCGAGGTCCGAAACAACTGCCCGTACACCGTTTG GGCGGCGTCGACCCCCATAGGCGGTGGCCGACGCCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCA CGAGGTACTAAAATGGCACGTATATGGGGTCGTACAAATTGTAACTTTAATGCTGCTGGTAGGGGTACAT GCCAAACCGGTGACTGTGGTGGAGTCCTACAATGCACCGGGTGGGGTAAACCACCAAACACCTTGGCCGA ATACGCATTGGACCAATTCAGTGGCTTAGATTTTTGGGACATTTCTTTAGTAGATGGATTCAACATACCG ATGACTTTCGCCCCGACTAACCCTAGTGGAGGGAAATGCCATGCAATTCATTGCACGGCCAATATAAACG GTGAATGTCCCCGAGAACTTAGGGTTCCCGGAGGATGTAATAACCCTTGTACTACATTTGGAGGACAACA ATATTGTTGCACACAAGGACCTTGTGGTCCTACATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGAC GCCTATAGCTACCCACAAGATGATCCTACAAGCACTTTTACTTGCCCTGGTGGTAGTACAAATTATAAGG TTATCTTCTGTCCTAATGGTCAAGCTCACCCAAATTTCCCCTTGGAAATGCCTGGAAGTGATGAAGTGGC TAAGTAGAACGGTCATTCCTCTAATAAGATCATCTTTGGTCGAATTATTGCGTGAAAGCGTTAGTGTGGG ACAATTTATTTTGTCTCGCTTTTATAGTTTACACACTTTGTTTATTTTGGTTGAAGTGGTCAAGAGATGA TCT
nuccore fasta 1000000 multi 5
abscisic acid-activated [Nicotiana tabacum L.=tobacco, leaf, Genomic, 3033 nt] GenBank: S40046.1 GenBank Graphics Related Sequences
>gi|251138|gb|S40046.1| abscisic acid-activated [Nicotiana tabacum L.=tobacco, leaf, Genomic, 3033 nt] GTCGACTTTTGTATCAGTTGATGTTCTGGTAGTATTACACTAGACTTAGTTTCGTAACTGATTGTTTTAT AAATTTTCCGGTAACGTCCAAATATGTCCTTACCGTCACATAGTTGGTCTATATATCCCTTTTTGAAATA GAATCCATCCAAATTATTTAGCTCTTCCGTTAATTATACTAAAATGTATGACAAACACTATTTCCTTTTT ATTCAGTACTTTTTTTTCTTTATCAATTTAACTTGACAAAACTCATGAATTCCTGTTAATTTTACTATTA CAGGCCCCACAGGTTCCTTCCTGACCTGAAGGAGATGAAAATTATCGGAGAAATTTTTTCGGTGGTGTTA ATTGGGTGAAGGTAGGATATGATTATTCAAATTTTGAAGATCTTGTTCCTTTCAGATCTGAAGCTCCACA GTGAACAACTCCTTCAAAATCTGAATAATTATATCCTACCTTCACCAAATTAACACCACCAAAAAAATTT CTCCGACAATTTTTATCTCCTTCTTTTCAGAAGAACTAGAGCCTTCTCGGTTGGAAGGTTTTGGTGGTGC AAGTTTGATTTTTAGGAATAAAATCACGTCAAATTGGTAACGAAAATGGAGAAGGCACCGGAAATGGAGG AAACCGGATATGGGAGAATGAAAAAGGGAAAAAAAGATAAGAAAAGAAAAAAGAAGAAAGAAAAGAGAAA GGTAAAGAAAAAAAGTACTAATAAAAAGTAGATAGTGTTTGTAATACACTTTAATACAATTAAAGAAAGA GCTAATTAGTTTGGGTGGATTCATTTTTAAAAAGGGCAACTATGTGACGGTAAGGACATATATGGACCAA CTATGTGTATGGTAAGGGCATATATGGACCAATTATGTGACGGTACGAGTATATATGAGCTAAAGTATTA ACAAAGGGTAAATGTGCTCAATTTCGTATATTACAAAGCCATATTTGGACCTTTTTCCGTAAATTTTATG TAGATTTAGAAAAAAGCAACAACCTATAAGGGGTTGGTCTTTAAATATTGTCTTCATTTTTTAATGTACT TAAAGAATGAGCTCTGGACCTATATAGTTCTTCAGAGATTTTTCTATTGGATCGCTAGAATTTATGTTAT ATTTATTCTACTTTTATTGTTAAGTGTTCACAAATTTTATTCGATTAGCATGATTTTGTGCTAGTTTTAT TGTTAAACAAATTTCACAGAATCGGCGTAACTTTATTTTATCTGCAATCGATGTACTTCTTAAATTGTTC ATTAAATCTACCTGACTGGTATAATTTTTCTGTGTTCTTCTCTGCGCTTATTCTACATCCAGAATAACGA TATCTAATTAATGAGCTGCTATATAAATCGATGTAATAGTTCTCAAAAAGAAAATGAAGGAAGAAAAAAC TATGTGGTGGGACAATATAACATCATCTATATATAAAAATTAAAGTGAAATCCAGGATTTCAGTATTAAA ACTACAGGAAAAATTTATGATCGGTGCAAACTCCATAAAAAATTTCGGAAGTACAAAATGTGGAGTTCAA ACTGATAAACAAACTCTAATAAATTTCTTATAATTTTTTTATATTTTTGTGACGAATATTATTGTTTGAG
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TTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCTGCCAAAAAGTGGCTA TCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATAT TGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATTGACTTCTATATTCAT AAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCCAAAGCATCCTTGAGA TATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTACGAAAAGCCGCCATT CCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTAAAAAAAATGTCCAAC AACATGGGCAACTTGAGATCTTCTTTTGTTTTCTTCCTCCTTGCCTTGGTGACTTATACTTATGCTGCCA CTATCGAGGTCCGAAACAACTGTCCGTACACCGTTTGGGCGGCGTCGACACCCATAGGCGGTGGCCGGCG TCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCACGAGGTACTAAAATGGCACGTGTATGGGGCCGT ACTAATTGTAACTTCAATGCTGCTGGTAGGGGTACGTGCCAAACCGGTGACTGTGGTGGAGTCCTACAGT GCACCGGGTGGGGTAAACCACCAAACACCTTGGCTGAATACGCTTTGGACCAATTCAGTGGTTTAGATTT CTGGGACATTTCTTTAGTTGATGGATTCAACATTCCGATGACTTTCGCCCCGACTAACCCTAGTGGAGGG AAATGCCATGCAATTCATTGTACGGCTAATATAAACGGCGAATGTCCCCGCGAACTTAGGGTTCCCGGAG GATGTAATAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACACAAGGACCTTGTGGTCCTAC ATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGATGCCTATAGCTACCCACAAGATGATCCTACTAGC ACTTTTACTTGCCCTGGTGGTAGTACAAATTATAGGGTTATCTTTTGTCCTAATGGTCAAGCTCACCCAA ATTTTCCCTTGGAAATGCCTGGAAGTGATGAAGTGGCTAAGTAGAGTGGCTATTTCTGTAATAAGATCAC CTTTTGGTCAAATTATTCTATCGACACGTTAGTAAGACAATCTATTTGACTCGTTTTTATAGTTACGTAC TTTGTTTGAAGTGATCAAGTCATGATCTTTGCTGTAATAAACCTAAGACCTGAATAAGAGTCACATATGT ATTTTTGTCTTGATGTTATATAGATCAATAATGCATTTGGATTATCGTTTTTATATTGTTTTTCTTTTGA AGTTTTAGTAAAGTCTTAAGCTT
osmotin {promoter} [Nicotiana tabacum=tobacco, Genomic, 2051 nt] GenBank: S68111.1

>gi|545027|gb|S68111.1| osmotin {promoter} [Nicotiana tabacum=tobacco, Genomic, 2051 nt] GTCGACTTTTGTATCAGTTGATGTTCTGGTAGTATTACACTAGACTTAGTTTCGTAACTGATTGTTTTAT AAATTTTCCGGTAACGTCCAAATATGTCCTTACCGTCACATAGTTGGTCTATATATCCCTTTTTGAAATA GAATCCATCCAAATTATTTAGCTCTTCCGTTAATTATACTAAAATGTATGACAAACACTATTTCCTTTTT ATTCAGTACTTTTTTTTCTTTATCAATTTAACTTGACAAAACTCATGAATTCCTGTTAATTTTACTATTA CAGGCCCCACAGGTTCCTTCCTGACCTCAAGGAGATGAAAATTATCGGAGAAATTTTTTCGGTGGTGTTA ATTGGGTGAAGGTAGGATATGATTATTCAAATTTTGAAGATCTTGTTCCTTTCAGATCTGAAGCTCCACA GTGAACAACTCCTTCAAAATCTGAATAATTATATCCTACCTTCACCAAATTAACACCACCAAAAAAATTT CTCCGACAATTTTTATCTCCTTCTTTTCAGAAGAACTAGAGCCTTCTCGGTTGGAAGGTTTTGGTGGTGC AAGTTTGATTTTTAGGAATAAAATCACGTCAAATTGGTAACGAAAATGGAGAAGGCACCGGAAATGGAGG AAACCGGATATGGGAGAATGAAAAAGGGAAAAAAAGATAAGAAAAGAAAAAAGAAGAAAGAAAAGAGAAA GGTAAAGAAAAAAAGTACTAATAAAAAGTAGATAGTGTTTGTAATACACTTTAATACAATTAAAGAAAGA GCTAATTAGTTTGGGTGGATTCATTTTTAAAAAGGGCAACTATGTGACGGTAAGGACATATATGGACCAA CTATGTGTATGGTAAGGGCATATATGGACCAATTATGTGACGGTACGAGTATATATGAGCTAAAGTATTA ACAAAGGGTAAATGTGCTCAATTTCGTATATTACAAAGCCATATTTGGACCTTTTTCCGTAAATTTTATG TAGATTTAGAAAAAAGCAACAACCTATAAGGGGTTGGTCTTTAAATATTGTCTTCATTTTTTAATGTACT TAAAGAATGAGCTCTGGACCTATATAGTTCTTCAGAGATTTTTCTATTGGATCGCTAGAATTTATGTTAT ATTTATTCTACTTTTATTGTTAAGTGTTCACAAATTTTATTCGATTAGCATGATTTTGTGCTAGTTTTAT TGTTAAACAAATTTCACAGAATCGGCGTAACTTTATTTTATCTGCAATCGATGTACTTCTTAAATTGTTC ATTAAATCTACCTGACTGGTATAATTTTTCTGTGTTCTTCTCTGCGCTTATTCTACATCCAGAATAACGA TATCTAATTAATGAGCTGCTATATAAATCGATGTAATAGTTCTCAAAAAGAAAATGAAGGAAGAAAAAAC TATGTGGTGGGACAATATAACATCATCTATATATAAAAATTAAAGTGAAATCCAGGATTTCAGTATTAAA ACTACAGGAAAAATTTATGATCGGTGCAAACTCCATAAAAAATTTCGGAAGTACAAAATGTGGAGTTCAA ACTGATAAACAAACTCTAATAAATTTCTTATAATTTTTTTATATTTTTGTGACGAATATTATTGTTTGAG TTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCTGCCAAAAAGTGGCTA TCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATTGACTAAAAGAGATAT TGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATTGACTTCTATATTCAT
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AAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCCAAAGCATCCTTGAGA TATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTACGAAAAGCCGCCATT CCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTAAAAAAAATGTCCAAC AACATGGGCAACTTGAGATCT
N.tabacum ap24 gene GenBank: X65701.1 GenBank Graphics Related Sequences

>gi|19782|emb|X65701.1| N.tabacum ap24 gene ATATTATTGTTTGAGTTTTATTTTCACATTAAAAACTAAATATTGAATAGCTTTAAAATGATGGCTATCT GCCAAAAAGTGGCTATCTGTCAATTTCTTGCGAATTAAAAAATGGTATAGATAAAAGAAAGCAAGAAATT GACTAAAAGAGATATTGTTACAAGTGTCACGTTACAGAGATTATAGGTCAGCGTTATTACCAAATAAATT GACTTCTATATTCATAAAATAATTAATTATTAGGCGGCTCTTATGTTTAAGCGCCGCCTCCATCTTTGCC AAAGCATCCTTGAGATATATCCGTTTATTAGTCAAATGTTAATAAATATTTATGATTAATATCCATAGTA CGAAAAGCCGCCATTCCCCTATATAAACCACTAAACAATTTGTCACTATATCCAACAACCCAACTTGTTA AAAAAAATGTCCAACAACATGGGCAACTTGAGATCTTCTTTTGTTTTCTTCCTCCTTGCCTTGGTGACTT ATACTTATGCTGCCACTATCGAGGTCCGAAACAACTGTCCGTACACCGTTTGGGCGGCGTCGACACCCAT AGGCGGTGGCCGGCGTCTCGATCGAGGCCAAACTTGGGTGATCAATGCGCCACGAGGTACTAAAATGGCA CGTGTATGGGGCCGTACTAATTGTAACTTCAATGCTGCTGGTAGGGGTACGTGCCAAACCGGTGACTGTG GTGGAGTCCTACAGTGCACCGGGTGGGGTAAACCACCAAACACCTTGGCTGAATACGCTTTGGACCAATT CAGTGGTTTAGATTTCTGGGACATTTCTTTAGTTGATGGATTCAACATTCCGATGACTTTCGCCCCGACT AACCCTAGTGGAGGGAAATGCCATGCAATTCATTGTACGGCTAATATAAACGGCGAATGTCCCCGCGAAC TTAGGGTTCCCGGAGGATGTAATAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACACAAGG ACCTTGTGGTCCTACATTTTTCTCAAAATTTTTCAAACAAAGATGCCCTGATGCCTATAGCTACCCACAA GATGATCCTACTAGCACTTTTACTTGCCCTGGTGGTAGTACAAATTATAGGGTTATCTTTTGTCCTAATG GTCAAGCTCACCCAAATTTTCCCTTGGAAATGCCTGGAAGTGATGAAGTGGCTAAGTAGAGTGGCTATTT CTGTAATAAGATCACCTTTTGGTCAAATTATTCTATCGACACGTTAGTGTAAGACAATCTATTTGACTCG TTTTTATAGTTACGTACTTTGTTTGAAGTGATCAAGTCATGATCTTTGCTGTAATAAACCTAAGACCTGA ATAAGAGTCACATATGTATTTTTGTCTTGATGTTATATAGATCAATAATGCATTTGGATTATCGTTTTTA TATTGTTTTTCTTTTGAAGTTTTAGTAAAGTCTTAAGCTTAAACCAATAAACAAAACAAACAAAAAAAAA GGTATTGATAATATTCTAGCATAGGACCCAAGACGTTGAAAGTATAAAATGGTAGGATAAGATAAACTAT TTGGAGCTC
5. Solanum lycopersicum DNA, chromosome 8, clone: C08SLe0023D06, complete sequence GenBank: AP009601.1 GenBank Graphics Related Sequences
>gi|165263516|dbj|AP009601.1| Solanum lycopersicum DNA, chromosome 8, clone: C08SLe0023D06, complete sequence GAATTCTTCATATAATTAACTTCTTGATCATAATTTGCGACTAATTAAGCCACAAATTGTCACTAATGCC ATAATTAACGATTGATCAACTTGGGGTTCCACTTCTAGAGTTAACACATCATCTCCATATGCAAATCCTC
Page | 17
GEN 3040
TCGACGATTGCTTTTGTTTTAACTGCGACAAAAAAAAAATCAATATCAGAATCTTTATACTATCAGTATA TATAACTTGGATTAAATTTTTTTTTTAAAAAAAACTTTATATGAATTTATAATTTTTAAAGGACCTACCT CAGCAACAATTTCTCCAGTACTATTTGTAACTTTAAATGATGATTTCTTGTCCAATTTTTGAATCTTATA ACAACTTTCAATTGATTTTTCATATCCCAAATTCACATTACATATAACATTTCCTCTTGAAAATGTGCAA TTCTTTTTTACTTGAAACCATGGCCTTCCTTTAAATCCACCACATAAATATCCATTCCATCTACCAAAAA CTCTCAATTTCTGCAATTAACATAAAAACAAAAAAAATAATTAATTTCAACTTCCATAACATAAAATATA CTCATTCTGTCTCAATTTATGTATTAATACTGTTCGAATTTCGAGAGTTAAACAGATCCTATGTTTGAAT TCATAGTCAAAGTTTCTGGAAATTCAAACTGTGTCACATAAATTATAACGGAATAAAATAATTTATTACC TCTTTTTTGATGGAAAAGAGAACTTGTCCATTAAGATCCATAAGAAAAACTTCATTGCTACATCTTTCTT GATAATTATCAACTCTAAAACAAAGTTCACCTTTGGAATTAAAAACAGTACATCCATTTCCATGAAAAAC TAGTGATTTCATCCATATTGTAAATATTTCTTTATTAGATGTAACATAAGGAGATGAACAAGAAGAAGAA GAAGAAGGGGAAGATATATTTTCTGGATGAATTTTACCCATAATTAAAGTAAAATTAAATTTATAAAAAA AAAAATTGTAATTTTGAAGAAATGAAATCGCTTGAGAGAAGATTTTGGAATTTGATTTTTGGTGTGATGA GTAATAGAGAAGTTGAAAGGGTATAAATAGAAGTTAAAGAAACAACCACTGAAAAAAATAGTTAAAGAAA TAATCAAGAAAGTAGAAACATTTGACTTTATAAAAACGTGTAATGTTGACGAAGTATTTTTTTTTATTAT CATCACCATTTAATATTTTGATATTTACTAATTCGATTATTTTAAATTCGTGTAACGCAAAATTTATTAC AGAGAAAATTATTTTTTATTAGAAGTTTTTTTATTATATATAGGCTTGAATTCGAGACTTCTAATAAAAA ATAGAGAAATTTAATTCATTATACAGTGATAAAACTACGTATATAAGAGATTGAGTTGATCGATTGATAT TTTTCACTGAAAAATCACATTATATATATAAAAATATGATTATTTTATACATTTATCTTTTCATATTTTT GATATTTCTCGATAAAACTCTTTTGATATTAAATGATAGGCATTGATATATAGAAAAATAATAATAATAA TGGTTATACTTAAGTTTTTTATTTTACACATTCCATATTATTGTAAGAAAACAAGTCAATTTAATAAAAA ATTACTTATTTGAGATAATTTCAAAATAATATTGTGTCAAAGTCAGTTTAAATAAAAAAAATATTTATTC AAAGATAATTTCAAAATATTATATCAAAACTTATACATAAGAAGACATAGACAAAGAGGTTTTGTCTTTA TTTATTCACATCCAGTCTGTCTTTGGGCAAATAAATAAAGTAGAATATGACTCTTCATAGAGTTTATTTT TATTCTTATAATATGTGTGTCTTTGTATACCCTATATATAATATGCTTTTTTTTTGCCATGTGAGGTGGC CTTATGGAGAACCACTAAACCACAAATCATCACAACATAGATTCATATTTCTCCTTTATATCCTCCTTCA CCTAGGAACTAAAATATATTCTTTTTATAGTTTTGTGTCAAAAGATTTTTTTTTTAAAAAAGTTCCAAAA TGTGGTCTATAATAACTCACTAGGCAATATATCAAAATCTTGGAGGTTCACCGTGGAAAAATATAGTGGG TCGAAACTTATCCATTTTAATCAGATATTTAGAGTTTGATTTTTTAAAATGAGAAAATTTATATTAGAAA GTGTTTTCTTTTTTTAAAAAAAAAAAACTTTTATGCAACGTGAATATAAATTAATGTTGTAGTGAACCCT TAAGTTGTAGACAAATGTGTGAAAGTATCAAACTGATTGACAATATGAGTTTGCTATCTTTTTATACGAT TTGAAATTATTAAGTTTTTAAGTTAAATTAAATTTACGATTTATTTTCTTGATATATTAAAAGAGTGAAT ATGTTATCCACGCTTCAAATGTACATGTTTTCTTCTATGATTTTTGCCCAAATTTTGAATTACACAACAT TGGGCTCCAAGATATATTCTTTCACGTTTCAAATGTCCATTTAATTTCTTAATTATAACATGATATTTTC ATATTAGTCAGAATTTTAGTGATTTTAATAATCATTATGACTTTTTTAGATTATTAATATTTGTAATTCA TAAGTCAACTCCTATTTTCTTAGTGACATATTAATCCCAAATCAGTGGGGTTCCACTTACGTTTGTGTAA CAACCACTAAATACTTCTTCAAAAAAAAAAGAAAAAAAAAAGAAAGTGGAAGTGTAATAACTGTTCAATG TCAAAAGAGACACAATTAAATTAGAGATTTTAATATATGCACTTAAGGGTAAATGAAATTAAAATAAAAT GGTGTCAAAAGAAAGGATTATCATTGATTAATTTAATAAATAGTTGTTGTTAATTGTTTGTGTGCATCGT TTTGCGTGCTTATCCCTTAGATGAGGGAAAATTAGGTATTTGACACTGACTAACTGTTTATTTTATATTT TATATAAATTTGAGATGGTAAAAAATGTAATTTTAATATAAATACAAAATTCAAGAAATTATAATTCTAC AAATATACCCACCCTTAGATATCAATAAATTTTATAACAGAATGGTAATATTCAAATATGTTCCTTTTGA TGATAGATTTCTTTTTATTTTTTAATAATTTAAACTCGAGATTTCTAGGCCGTAATAGTTACGATCAAAA ACGAGTAAACTCACACATAAAAAAAGTTGTTGATGATGATTGTCCACTGGGAATTTTGCATCATGAAAAA TTTAAAGTTAAGAGCTGGTAAAAATGACAACCATATTCTTCTTTTAGTGGAATTATAATAAAGACAGGCA CCACCAGTAACATATTCTCGACTTATATATAGGATGTGACATTTGATAATATTCTTTTAGTAAATTTTAA GTTTAGCAAATATTAAAATTATATGTATATGTTATAATTATAATTTGTATAATTACATGAAGTGTCAGAT CTTTATAAATTATTGGTAAGTCTTTCGTTTGTATAAAAGCGCAACGATTATATATATATAGGTTAGACAA TTGTACATACGTAATTACTATGTACATATACCAATGATTGTGTTTGTATATATGTATAAAAATGAATTTG TATATAATTGAATCGAAATAAAATATTTATATATCACATCTCTGGATTTATACAACACAATTATACAAAT TGTAATTTGTATAATTTGTGTTTGTATAAGAGAAAGACAAAAGAGAACTGGTAGAAGAAGATCTGTATTT GTATAATTATAAATTTATAGGATAAAAATACATGTATTTGTATGTGTATATACAATTTTAGTTCGCTTTA CACAAACGCAAACGCAATATATAGGCGTGGAAGAGCGGCGAGTGAAATTCTTTGGAGAGAGAGGCAAATG ACAAGAGTTTGATATGAATTACAATTAAATGAAAAGTATAATTATAACATTAAATTTGAACTAATAATTT GCTATTTTATATAATTTTTCCTAATTTTAAAGTTAATGAGTTGTAAAAGAAAAAGATATGTTTATCGTAA ATAATTCAGAAAGAAAGTATGAAACAAATAAATTTCAACGAAGAGAATAATAAAAAATCGGGAAAAAAAT ATATTATCCTTTTAAGGGATTTTTCTAATATTGGACGGCAAACTCCACTATGTACCGACAAGCAAAGTTA
Page | 18
GEN 3040
ATTTGATTAGAAATTATGATGAATTTGACCTTAATTTGATATTATTATTATTATTATTATTATATAACAC TGAAACTAGACTAAGGTGAAAAGTGTCATGATATGTAATAATTTAGAAGCTTGAATCTGTCATAGTTCTC AAACATATATATTTTATTTGTTTCATAATTTACTTTTTTCTTTCACGATCAAATCGTTTTAGTTTGTACA TAAAATTTATATATTTGTAAATTATATAAAATATCTTAAATCATAAGTGATAATTCAAAATATTTAAAAG ATATATAAAAAAAAATTATGATAAAAATAAATTTATTTGAATCTAAAAAGCTAAAAGGTACCACATAAGA AATATGAACTTCTTGTTTACAAATATTTAGAAAAAAATTAACAAGGTTTTATATATTAATTTAAAAACTT TCTTGTTAGAGTTTACCATAAATATCTATGCGAATATCTTTTTATAATCACATAAATATTATGACATGAC TAAAATTATATTTTTTTCTTATAATTATACAAAAGTTATAACATGTCCAATATTACGAGATAAAAAATAT CTTCTCTATTTTAGACTACATTTAAATAAAATTTACATCTCATATATTAATTAAAACTATATATAGGGGA GTATTATTCTTAACACTGTTTTCTAAAAAAAAATTTGGGGAGAGTTTTGCGGAGGGGTGTATGAAAAATG TTTGAAAATGCAAATTGAAAGACATATTCATAAGGTGTGAATCTTAGAATTGGGACGTACGCTTTATTTT TCTTTTCTTTTTTATAGTTTTTGAACCTTTTTTTGCTTTTAATGATATAATGACATTTCTCAAATAGTGA TTATAATACTTCTTTTTTTGTTATTGATTTATTAATATTTTTCTTAAATAAAAATTATATTATATTTTTC TATATATTAGAGGTTATTCATGAAAGATTACTTGAAAATAATTAAAAAATTTATTTTGCATATTTTCTTA AGAATAAAACTATAAAATTGAATATACATGAGATTATTTCCCGTAGATCTATAAGACTTCATTCTGTATT TTAAAAGTTTTATCTCGTTTCATACTGCATAAAACGTCTCATCTCACCTTTCATCATATAAATTATTATT TCTCTAGATTTCATTATCAAATAATTATATGAATTTCTCAATTATTCAATATATATTTTTAGTTAATGGT ATAAAATATGTGATCATATTTAATTATCGTAACATGACAGAATAACAGTCGGATGATGGACGTGGCATGT TTTTGAACTAAAACTTATCACATGTTAACTTTTAAAAAAGTTGATCCAAATGCTTAAAATATGATTGGAT AATATATATAGGACAATGAGTAAATTTATAATTTTTAGGATAATTTGTATATAAATAAAATAAATTATAA GTGCGGGTTGATGCATGCACACCAAAAAGTTTGAGTTTGACAGAGCCATTTATATTTTTATAATAAAATT ATAATTCATCACTATGATTAGAACAAATTAAATATCCACTTAGGGTAATTTTTTAATTTTTTTTGGAAAT AAATAATTAGTAGTGGTGCTAATATCTATTGAAGAAATTTAGGTTGTCTTTTTTACTTATTCTTGATAAC GTATTAATGTGCATGCTGACATTGGTGAACTTAATTACGTAGGAAATCATTTTTTTAATGTTTACAATGG TTTAATGTTAATTTAGTCATAATGTCCTAGTAAGCAAGCATTTACTTTACAACCTGTTTATAATTACCTA ATTGCTTAAACCATCAAGCATACCATTTTGAACACCACTAACTTCTTTCCTTTTTCGTTTTCGGTGTTCG ATATTTATATTGTAGTCTGATTAAGTTAGAACTTTATATTTATACTTTTTGCATTCGATGTTTAACGATT ATATTGAAGTTTGATTAATTAGTAAATTTAAATTTGCACATTATCAAATACACTCGATATTAGATAACCA CATTGCAGTCTGATTAATTTGATTTCGTACATTACATCGTCCATTTCTTAAGGTGACGCTTTCATTTTCT CCCTATTTAAAAGGGACAATTTAATGAGGGAGTCAACCATCGATAAATCGTAAATTGAAATTGATCATAC TCTGATATCATGTTAGAAATGAATCTTGAACCGAACTCAATAAAAAGAAAAAAAAAGTCCACAAAGTGAA TATTGTTCATATAAGGGAGACCAAATCTCTTATTCCTACTCAATGTGGGACGGTCAACAAAAACATAGAG ACTTTCGAATATTAGTGAAGAAATTTTAACCATCCTAACATAAGTCATATTTTTTAAAAAGCAAATGCCA CTTATATTAGAAATTACCTTATTCGTTAGGCCACGTACAATACACCATAACTCACGTTGGTGTGTCGAAC AACATAATTGATATTTGACTATTTTATTATCTATATATTCAAACATCAGCAGTTATTCAAATCTTTTTGG AACATCAAATTTTCAAAAGTAAATAAAAAGTAACTTAAAAAGTGTAGATAGACAAATTCAACTTGAAATA TATACGAGAAAAATAATTTAGTTGCTGATGCTCAACTTAAAACAAGAAAAAATGAGCTCATTGGTAATCA TGAACCTAAAATAATATCAACAAGGAATACGTTTGAAGTAATTTATCGTATGTGATTTGGATAATTATTC CTTTATTAATTGTTAATTAGGATGATGTGTTTTTGTTCCTAGTATATTAAAATAATCAAATTTATTTCAC AGTTTTATATAAAATTTTAAAAAAAGAAGACATCATCTTAATTCTTAGCTACATCGTCTTTTCATGAACA AATTTGATGATCTAGTAATATTTATATAAAGAAATAATTTAAAGTAAATAACAATTTTTTGGATATAAAT TATCAAATGTTGTAAATATTGTCATTACAATATATATAAACAAGGATATTTTAATAGTTAGGTTGTCTCA CCAGGTCCACCATATAAAGCGACGTGCTCAAATTTTCCTTTCGTTGGTGGAATATCGTAAACCCTATAAA AATCGGGATTATCAGTGTGCGGGATCGTATTTATCATTATCGTCTCACCTCTATAGTTTAATGCAATAAT TTTATTGCATGAACCATCGTACCCTTGCTTTTTAGTGGGGAAAAAACTTGAACCCATTGGTGGTTCAGGT ATGTTTTGTGGGCTATATGCTACTCCTCCCCATTCTATGTGTGTAGCAAAGCTAGCTAACTGAGTGAAGA TGTTTTGAGGCCAAAATCCAATTTCTCTATGCGTTTCTTCAACCAAAACCCACCAATTTCCGTTGGCTAG ATCCTTTTATTTCGAAAAAAAAAAAGATATATATATATATATATATTAGTATCTCATTTTATATTCATTT TTTTTTTAAAAAAAAAAACAGTTCTTCAAAAGACAAACATAACATATACCCGATCGAGGTACATTGAATA TTCCCACAAGTTATGAATATCACCACGTTGTGAAACATTACTAAATGGCGCTCCAATGCCTATCTCAGTG TCTACCAGTACAAATCCAGGACATAGTGTATCAAAGCAACCTATATTACCAGCCTATATATAGACAGTAT TAAAAAATTTTATGAACGCGAAATAAGAGTTAACAAAATATTCATAAAAATATCAACTAACCTGAAAATG TATGAACATTCTAGTTAGGGTGTCGCCATATAATGTTGGATCCACCTACGTACACAAAGAAATAGAACTA TCATCATGATAAATTGAATGGTTATACACTTTCTAATCTTGCATTATATTCGAGAGGTGTCATATTCAAC CCTCTAAATACGAGATAATTTATTTATAACAAAATAAGCTATAGACAAAAGCTTACTCTCCAACCGATTT GTATAATGTCCGATGATTTTTGAACTTTCAATCGACATCCACTGTGTTGTGGAGCTTTAACAGAAGGATT CCATAAAGTAGATGTCATTCCAGCTCCCCCAAACTTGTAATCCAAATCATCACGAGTTGAAACTATTGCA
Page | 19
GEN 3040
ACCTTCAATATTTTTATAAATTCTTTTTATTAAGAAAAAAAAAGACATCAAAAAAAAATTTAGAAAATTA AAAGTTCATATACCCTATATCCTTGTGTAGATAATAATCTTCTTTTCAATCCACTGTGATTCCTGGTCTT TGGAAAAGTAATAAATAAAAACAAATGAGATTTGATTTTAATGGTGAAAACTAAACATCCTATACGTTTA TTTATATTAATATAAATTATATATATAATTTAAGATCCTATTGATGGAAATGAATTATTAATGATACATC AATAATTAAATTGATTTGACATTATCTTACCTGAGAAAATTGGGTATGAACTTCTGGTGGTGGCATATGT TTTTGTCTGATAAGATCATCTTTTGTAATTCTTTTAATCGGAACTGATCCAAAAGGACAACCACCATCAT TTAGCCATATCGTCGATGACTCTTTAGACTTTGAAGTACCTGAAGATTGCTGTATTCTAGATAACGTAGG CTTCATCTATTCACTCAAAATTAAAATTCTAAATCAATACATATATAAATCCTTGTTAAAGGTCGATAGT AAAATATAATTTGGAACGACATACATTTGGATGAAAATTGTGGTTCTTCAAAGATGGATGATCAAACGCA GGTTGCTTGTAAAAATCCACACAATCATATATATCTCCATATGTAGTCTGCGACTCAAAATTTAAATAAA TAAGTCATTTAGGTTATAACAAATGCATATATATTGTTATTCAAATCCTAATATGAAAAAATACCGTAAT TGTTTTGATTGCAGGCTTGTTCAAAAGTTTAAGTTGACTTTCTAACTCTTGATCCTCTAATTCGGATAAA CTTATTTCTCCTTGAACCTCATTATAACTCAGAATAAAATATAACACTAAAATTTGTTGAATAATTCCTT GATTTATCTGCATTGCCTGAACTTTGAGAGAGCATATGTTAAATAGATTTAGAGAGGAGATTAAAAAAAA AATTGTTATGCAAGTTAAGTAATGTAATTTGTTTTAAAAGCAAGATTATCTTAATTTTGTTAAGAATTTA TTATATTAAATGCAAGTTGACTGTTTGCTTTAAACTTTAATCTACAAAACAGCTGTATCATTTGACCACT GAGAATATTATCTCATTTTTAAAAAAAGGCAAACTCAATTTATCCTGATGTATTGTTTTTTCTTTTATTT CAATTTAAAAGGTTGATGTGTTTCTTACAAGCCATAACAATAATAATATTTCATTATATTTAATATAATT TTATAATAATTGATATATTTTGGTTTTGTGTTTTATGTTAGTATATATCAAGACATAACACCAAATTAAA AATTATTCACCTATATGAATATATCAACCATCAAAAAATTATATGAACAAGCGATTTCGATTTACGTATT ATTTTCTATAATTATTCTTTAAAAGAAATTACATGATAGCGAGATTTTGCAAGTGTATAACATATATACA GTTATACATGTATCTGTATATCTAAAAAGTTATAATTGACATTACTCTCACATACCTCCGCGTGATTTAC AATAAATATGGGATAATATTAAGCCACTAATTATTTTATTTTATTTTTTGCAAAAATGGACGCTAATAAC ACTATTTACTAAATGTTGTCACAATCAAAATATTAATACAAATAATCCATAATTCCACCAGTCTAAAGTT TGTCTTTCCACTTAAATTAACGGGCCTAAGATTTTGCCCTCCCAGCCCAAAAAGCTTGAAAAGCCCAGAC AAAGTCTAGAAAAATAAAGCCCAATAGCTGTTGACATTAGGTAAATTGTGACTTCAGTGGACGATTATTT ATATTAAGGGAAAATTGTATATAATAGCAAATTAATAACCTAAAATAAATGGAGTAGCTAGGGTTTGATT TAATTGTGCTCCATAGCAAACATTTGCAAAAAATTGTCAGGCGCCTCTCTTTCAAATATCTCGCTCGCCA CTCTCCTCCAGTTTCTCGCTCGCTTCCTCACTTTTTATACAAACACGAGTGTATAAAAATTGTTTCTAAT TGTATAAAGCAGAGAAAATTGTATAAATACATATATTTTTGTTCCCCTCTCTCCCCTCTGCCAGATCTCG CTCGCCACTCTCCCAAATCTCGCTCGCCACCCTCGCCTTTCTCACTTATACAAACAAGAACCGAAATGTA TAAATTGCATTTCTGTTTGTATAAAACGTGAAAAAATTGTATGTACACATGCAAGTACATATATTTTCGT CCTATACATTTATAGTTATACAATAAAAGTACTCCCTGCCCAGTTTCTTAGCGACAAATGGTTAAAATTG TATGTGCCATCCTAAATTCTCGCAAATTTCTTGCTTCTCCATTCATTTTTTTTAAGCAAATGGTTAAAAT TATTAGACAAAAATAGTAAATGATCTAAACTTTTCCTTTTTCTTTTCTATCTTTGTAACTTTTAGGGAAT AGAGATTGAAATCCTGGCTATATAGATTTTTTTTAATATATATTTGATAGCTCAAAAGGATTTAAAAATC ACAATTCTCATCTAGACTTTGAAATATTTTTTTTTTTTAAAAATGGCATTTACAGGGGCCCCACAAAAAT GTAAAGTATGTGAAAAAACTGTTTATATTGCAGAAATGATCTCTACAAGCGGTGTTGCTTATCATAATAC ATGTTTTAGGTGTAACCATTGCAATGGAAGGCTTGCAGTAAGATCTTTTCATTTAATAATTATATCATCT TTTTATATATAAATTATAACAAAATATAATCATGAAATGTATAGGTTATCTATAGTTCTTAAAACAATTT ATTTCATCAACATTAATTAATCTTTTTATAATACATTTTGATTAATCTTTTTTTTTTAAAAAAAAAATAA AAATTTGCAGTTGAGCACTTATTCAACTCTTGATGGAGTATTATATTGTAAACCTCATTTTGAACAAATC TACAAGGAAAAAGGAGGAGCTCCCTTGAAGCATTCTGCATCATGTAATTTTATTTTAATTAATACACTTT AAAATAATTAATCCTACAATTAATTTTAATTATCTGATTATTTATTTGTTTCCTTATTTCTTGAGCAGTG GGAAGGCAAAATGAACTGGTATGCATCATTTATTTTACATTCTCTAAAATTATTTTTACAATCAGAATTA TGAGCTAAAATATTTGATAGACGTCCAAACGAAGTTAGTATTTTTTGTTTAAATTCATTAAATATGTATA AATATTGAATTATGAACCCAATAACTAAAACGAGCCATGAGTCAACGACGTATTTAGAATTATAAACTTT AAATTCTGACTTTGCCTGCGTTTATATTAATTTTCTGTATAATTTTATTGTTATTAACAGAACAGAAGCT CTAGTAAAGTTTCGGCTTTATTTTCTGGTACTCAAGACAAATGTGCAGCTTGTAAAAAGACTGTTTACCC CTTGGAGAAGGTTACTATTTTTACTTTTATTTTATTAATTTCTTTTTACAATTAATAAATATCAACAAAT TTCTTCTTAAAATTAAGACTAATTCATGTCAACAATTTTTTTTTTTAGTTTTTCTCTAGTCTTATAAATG TACGGAAGTAATTTGTTGTTTTGGAAAAAAAGAAAAAACTAATTTCCACATTTTGTTCATGCTAATAAAA TTAACAACATATCTCCAAGTTTTATTATCTCGGTTAATTCTGATTTTGATTTTTGTGATATATAAAACAT AATATCTTTAATTTTTAATTTATTAAAATGTGTATCTTTAGTTCATTTACATATCGGGAATATTTCATAT CTGAACTATTTTCAAAAACAATTTACCCTGCTCATCATCTGATTTTATATAGAAAGACTAATTTTATTCG GACATTGATATAGCGACAATATTTTTAGTGAAACTTAAGAAAAAGAGAGATTCCTGATCAACTTATTGAT GTTATGATTTATCTTAATATCGAAGTAAATCAATCAAAAGTATATATTTCAGGTAATTAAAGGACTAAAT
Page | 20
GEN 3040
TGTAATTTACCAATAATCTAGGAGCTAAGAGTGTATGAAACGGATGTATGAGATAACGTTCTCCGCTTAC TTAAAATCTTGAATCTGCTTCTGAATAAATATTTTTTTTCAGGTGACGGTGGACGGTGAAATGTACCACA AGATATGTTTTAGATGTGTCCATGGAGGTTGTAAACTTACAACATCATCTTATGCTGCATTTGATGGAGG TCTCTATTGCAAACCTCATTTCTCTCAATTGTTCAAAGAAAAAGGTTCATATAATCATCTAAGCAAAAAT GCTTCAATGAAGAAAAATGAAAGTTTAATCAACGTTGAACAAGAAGGTTCTAGTACAAACACAAGATGAT AAGTCATCCTATGTTAATTTACGTATGAAATTTTATTTAACGTCAATACGTTTATATTCAGATAATTGTA AAGGGTTATGTTCACTCATATGGTAGAATGACTAAGTAAACATGATAAGAAAAAAAATATTGTTTGGTAG TTCACTTATTTAGAAATGCATGGTAGAAAATAAATATATTAGAGTAAGAAGTTTACTAATTTTTTCCTAT AAAAACATAATATATTTTTATTTTCTTATCGATTTAATCAAACTTCGATACAAATAACTATCGAATATTT TTCAGTTTGAAAATTTAATAAAATGATGCAAATAGTATGACATAAATAAAATTAGGATCTACTTTATTTT TTATATTTAAAAAAGTGAATCTATTCTTATGCCTTTTTTCTGATGGCAACCCCACCAAAAAAGTGTTCAA ATAATAATTGGTGTTTGTCTTAGCATTGTTGAGAACTGCCAAATCCAATATTTTTTTTGATAAAATTGTG AATTTTAATTGCAAGTATAAGTTCGTAACAATTGAAAATTATTATTAATATTGTTAGTGAATTTATCAAA TTTAAATAAAAACAATTCTCTTCGTCTCAATTTATATGATATAATGTTACTGAATTCAGAAATTTAAAAA GAAAATATAATATGAGATATCTAAACAGCTCAAATACATCCATACCGATTTGAAAGGAATAAAAACAAAA AAAAGATAATAATCTAGACCAACTAACTTTATTAGGAATAGTTCATTTAGAATAAAATATCAATTACCTA ATCAAATTTGTTTCTTTTTGCAAGAAAAAAAGTATGAATCAATTTACATCTTTTTCTTGTTTTTATTCCT TCAAATTCTTACTACTCACTACATAATAAATTATTATATTTTAGGCCATAGACTCATAGTATTAAATAAA ATATTTTTGGTACGAATATAACGACCATTAATATTCTAGCTTTAGTTTACTTTGATTTCACAATTATAAA TCATATTGTATGCTCCTTCAAACATAATCATGTCTGCTATAAATTTATAAATAAATAATGCAATCACTAA CATATTACATACCTCCAATAATTTTACAAATATCTCAAAGTAACAATTAATAGTGAATATGAAAAATAAA ATACTAAGTGAATATTAGATAAAATTACTAAAAAAGTAGAAAATGAGAAAAGAAAATTAGCTTCGTTATT TTTAATTTTCAAATCTTTTTTACTGAAAAGCGATGTTATGCTATAACGAATAATAAAAAATAAAAGGAAA AATATATAATATCAAGTGTCACAAAGAGTACACTGTACAACCAAATCAATAAATTTAAATTCATAGCTAA GCAAATATTTTAGTAAAAACTATATATTAAAATTCTCTGTTAGCAAAAATCCAAACTTGTGAGCTACTTC TTTTTTCTTCTTTAATTCAGTTATCCACCATATCGTGTCGTGTAGTATTTTGTAAACAAGAACCAAAAAT AGAAATACTCTCCCTCCCTCGTAACTTCGGTTACACTTTTCAAATTTTGAAATTTAAATAAATTTAAATT TAACTATAAAATTTTTATATATTTTTTAGTTATTCTGAATTGTTAATTATATATTGTGACTTATAATATT TTTTACGTAGTTTATAAATATATAGTTTCATTTCAAATTTCTTTTTTGAAGATTTCACGTGCAAACTTAA ATGATTTGACTCTTGAAAAACGACAAGTATCTAGACGGAGAGTGTAACCTTTTTCTTTCCCATTTTACCC TCATCTTCTTTACCTTAACCCCACAAAATTGCCCTCTCTTTTTTCAAAAAAATCATCCCTTTACCTTCAC TTTTTTTCTTGTTTTCTTGCTTTCTTGCACTGTAAGTGCCACTTCTCTGCATAAAGTTTCCATCTTTTTT GTCTTTAAGAATTTCTGACACCCAATTGTTTCAGCTCTCTGGTCTCCTCTGTCTTTTTTTTTTCAATATA TGTTTTGGGGTTCAAGAAAGAGTAACAAGCTCAAAAGGACAAGAAAAGGGGAGGATGAGTACATCAACTA GTATTTTTGTGATAAGATGGATCAATTTTCTTACAATGGTATGAACCCCTTTTCATATTTTCAGTTTCTT TTGAGCTCCAGCTGAGTTTTCCAGCTCTGCTGCCTTATGTGAACTTGGGTTGTTTAGTTTTTGCTTCATT GAGTAGAGAAGATGTATTTGTAAACTGGTTTGGGGTTTGTGTTTCTTGATGTGGTTGGCAACTTTACTTT GATGTTTGTTAGTTTAGTGTTTCAATTTATTCTGAAATGCTATTGCTTTTGGTTCAATGTGATTGGTAGT TGTTCGGTGAGCTTGATTTATTTGTTGTTTTAGGAGAAAAGAACCAATTTTTACCTCCTTTAGTTCTAGT TTTCAATCTGAGGTAGAATATTTTGCTATGTGTTTAGACTTTAGCATGGTGGATAATGTTTTCTAAGAAA ATAAGTGAGTTTTTTACTTTTTTTGTGTGTTCGACAAGTAAGCAAAAGATATTGTCCTGAAATGAGAGGT GGGATTTGGATGTGAGATGCGGTGTGTTGGAGGGTGGGGAGAACACGATCAATGTGGAATATGACTAGAA AAACTTGTTTCTCGAAGTCATTTTCGTCATTTTTAAGAAGCTTGTTTTCCTAGAGAATGTGTTTTTCGAA ATATTTGACCAATCTAACGTGGGAAAATTGGAAAACATTAGTGAACACATGCTCTATCTTTTTTGTTTTC AAATTGAGGTTTTTCTAGTTCTTAATTTAGAGCAGGAAGTTTATCTTTTTTGGTGGTGTTTAACTGTTTT TGTACTCCATTTGGATCTTGAAAATGCAGCTGTTAGCTGTGGGCGTCATAGGTTTTGGGATTTGGATGAG CACTCATCATGATGTCTGTCGAAAGTCTCTCACTCTGCCTGTTCTAGGTCTTGGAGGAGTTATATTCGTC GTGTAGGTTTCTAGAAAATGTTTTCCTTCACATAAAAACGCACTTGTTCATTTAGTTTTCCCTTTAACCC TTAAACCACTCAAGGTTTGTTTATGTATATTGTGATAATCATTCTTCTTTACATGATTTTCAGATCAATT GTTGGGTTTCTTGGAGCATGGAAGAACAACTCCATCTTGTTGTGGATTGTATCCTCACTTTATCAAATGT CACATGAAGTGCAATTTTTTTTCTTATTCTTGATTGACATAACATTTTTTGGTTGCTTCTCCTTTACGTT ATATTTCAGTATCTGATCATGTTGTGCCTAATTTTGGTGGCAATATTGGTCTTCACCGTTTTAGCGTAAG TAATGCTTCTTGGAATTTTATTAAGAAATTGGTGCATTATGTCATTTTCTGCGCTAATTATCATGCACAC AATCACTAAATGTGTAATTTTTTTTATGGAGGCGATTTCAGTACAAAGTTAAAAGGCATGACCAAATTCG TAACTGTAGTTGTGGTCCACCAAGATATTTTAGTCATTGCTATGTTTATGATCCATTAAAAGTAACTATA GTTTTTTTTTTTGCATCCTGCTTGTTTTATGCTTTACAATTTACAAGCCATAATGGTGTATTATTGAGAG TTCTTTAGGAGTCTATCAATAGCTAATTAGCTACAGCACTGTTCTTCATTGCCTCTGGCTTGGTCCCCTG
Page | 21
GEN 3040
ACTGGAACATACTCTACTTCTTGGAGGCTGGTTTCACCGCCTTGGCTAAGTCACTTTTATTAGGGAAGAA TAGAAATACAATATCATGAATATCCTTAACTAGTTGTTCTATAAGTTCCAGCATTGTGTGTCTTGTTCCT GATCCATATTCCTTCTGCGCAGTGGGCTTATATTTTGGATACAACTTCTAATGTACTAAAATGAGCTTTA GAAAGAGTCAAATGGTGTATAGATTTGTTCAAATTTGGTAAGCGCCCTAGCACCGCCAGGTTGAAGCCTA TTTCCTTTTTCATTTTAGAGTAAAACACATAATGATTATTTTATAAAGGAGAATTCTTTCTTCAAGTATT TTTTTCTTTGGCCTGTTAACCAGCAATTGGTAGAGGCTTTAGCAGTTAAAGTAGCAACATTAGGTGTAAC GTTCATAGGTCTACTTGAATTCTTTTATAACCAAGAGACTGGTTGTTTCTAGCTCCAAAACTTGTTGAGA ATTCGCTCCTGTACCCTTCTCCTCTTTAATACCACATTTGGTTCGCAACAGGGTTAGAACTTATTGCTTG TGCTTACCACAAATCCCGCATCGTACTACCTTTGCTATTGAACCAAATCCCTGAGTGAGGACTCCTCTGA TTCTACTGGAACATGATACAATTTCCAATGTTAGTGTGGCAAGCCACAATGGCTCAGCTGAGACTTCAGC AGGTGAAGTAGTAATGCTACATGCCGTCTAAAGTGCATAAGACTTTCTTAAGTCCTTCTGGACCATATAG GAAGTTCCAGTGTTACATCAGAACTTATTGATATCAACCCAAGTAATGGTACTGCTCCCCTCTTTAGTAT TCGATGGGACTAATTTCGTGTATTGGTCAGTATGTGGATTAAGCCACTAATCTTGTGAGGAGACTGGTGA AGTAGTATCGGCAAAGGAAGAGAGACTTACATATGGTGTTCAGTGACCTTGAAAAGGCGTATGATAAAGC ACCTACAGTTTCTTTCCAGCCATACTGCCCGCTTAATGCAAAAAGGAATGCACAACTAATCTTCTTCTGC TTTTCCTTCTTTTTTTTTTCACGCAGTGACAAAAACTACACTAGCAAGTGATAGTCTAAGCATGAACCAG TCCAGCTTGGTTTTCAGTTAACTTCATTACTAGTTCTATCTATAATCTGTCTTGAACGTGGAAATCTAAA CTGGTTTTCCATATTCAATCCTTCATTAAACGGCAACTACTGCTGTGTCAGGGTTAACAGGCATAACTCA CAAGGTGGCAATTACTGTGTCTATGTGCATGCTGATGTCAGCAATGGCTATTACCTACATATTACTGTGA AACTTATTTTGGTTTGCTTTATCACAGGTTCATTGTGACGAATAACAGCTCAGGTCACAGTGTTAATGGA CAAAGGTACAATCACCTGTCATACTGTCTGTGATCACAATATCTCCTTCTCTTCATCCTTCAGTATCTTC CCCTTTATCCACTCTTTTTCTCGACCCACGTCTTGCTCAGTCTCACTTTGGTTCTCAATGTGTCTCAAAG ACATGGATTGTGCTTGTATATCTCATCATGACCGTCACAAGTTTTTCCAGTTTTCCTTTTTCCTTTTACT TCTGTGATATTCTTAGAAGAGTGAGGTCACTAATGGTTTTTGTGTGGGCAGGTACAAGGAGTATCAACTT CATGACTACAGTTCGTGGTTTCTAAAACAAGTAAGCACCTCTTGTATGCAAATACCACCTTTGTTACAAT TTATGTGACACACTTTCCTTTTTAGCCCGTCCCAAAAAGAGTGTCACATTTCTTTAATTTGAAACAATTT AACTTAAATTTCCATCTTACTCTTAATGAAATTATGTATAGCCACAGGACCACAAAAATTCAAGTCTTCC TTTCTTTCTTAAAATTATGTCAAGTCAATCGGTGCCACATAAATTGGGACGGAGGAAGTAATAAGTCGCA GGTTATAGGGCAAAGGCATCATGTCCTGATCGTTATAGGAGTTGTTTCAGATGCCTTAAGATGAAAATTT CATGCATTTATTAACCATTTGCTCATTTGTTTCAGCTCAATAACACCCATAACTGGAAGCATCTGAAAAG CTGTCTTGTCAAGGCTGATGACTGTAGTAACCTATCAAAACGATACAGGGTCATGTTCCGACTTGAACCT GTTCGTGATTTATATCTGTGAAATCAAATTATTAACGCTTCTGCTGTTTTGTTGCAGACTGCCAAGCAAT ACAAATTAGCAAAACTAACGCCTGTTGAAGCTGGTTGCTGCAGACCACCATCTGAGTAAGTTATCGTTAT TATTACTTGTTTGTTATTCCTTTTCTTTCTTTTGCTAGATATTTTCGTTTCATGCTACTCCGAGTTGTGC AAGTCACATATATCTTTTGTATATGTTGTTTCACCAATTCCTCTATATCTCGCGTGTTTGATATTGAAAT CCTTACTAGCATCATCCCATTCATTCCAAGGACTCAGAAATACTCTGCTCCTTTTGTAGATGTGGTTACC CTGCTGTTAACGCCTCATACTATGACTTGAGCTTTCATCCGACGAGTTCAAGCAAGGACTGCAAGCTTTA CAAAAACTCGAAGAACATCAAGTGCTATAGCTGTGATTCCTGCAAGTAAGAGTCATCTTCTGGTCTTGTC ATTCTTCGCTTAGGTTATGCAGCTCACGGCTCTTCTCCTACTCTATTATCTTACAGGGCTGGTGTTGCAC AATACATGAAAACTGAATGGAGAGTGGTTGCTATCTTCAATGTGATTCTCTTTGTCGTTTTGGTAAGTTC ACTCTTCTAATTAACATCTCAAAAAGTGAGCATAATGACTCTTGTTTCTTGTTTGAGAAGTACGGTTACA TTTATGGGCTAAGATTTGAAGTTTATGTGTTCTGAACTTGCCACCAAATCCATAACTCTTCTGAATTACT GAATTCACAACTACATATTTAATGGATTTCCACAAATATAGGTTCTTAGTTAAAGTTACTGGATTCGTCT AAACTCATAGCTAGTAATCTTGCTCCACTTCTGTGATTTCTCCGATGACTTGAATTGGTTCATATGACTT CTTCTCACTTTATCAGTCGATGATCTACTTCGTTGGATGCTGTGCAAGACGCAATGCTGCTCGTGAACGC TCTAAAATTTGAATTCCGCCACTGCAGAATCCAGATGAGATGAGTTGGTGTTCTTCAGCTGATGATAACA GTTCATGTTTTTCAGCTATTTGTTGACAAACTTTGTAAAGCTTTATTTTAGTGAAAAAAAAATGATTCAT GTAATATAAAAGATTTTTTTATGTTTAAGACCATATAATTTTGAAGTGTTTAAAAGTATACAAGCCTGAC TCTCTTTGTGTGGAAAGTAAACCAAGATAAAATATTACGATAATTGAAATACAAGCAATAACAGATAGTA ACATAAAATAAATGGACAAGGCCTAAAATACCCTTAAAGTATTGAAAATGGTACAAAATTACCCTTCATC CACCTATTGGCTCCAAAATGCTCTTTCCATCCACCCGTTGGCTCCAAAATACCCTTGTCATCCACCTTTG GATTCAAAATTGACCATTTTCAATTGTTTTAAGATTAAATTCTTTAAATATTTTTTTTTATATACTGGGC GTTCAACTATTAATTATAATTTTAATTTATTAATATAATTTATAAACTAATCCACTACCCATTCATTACA AACTAAACCTCACTCAATTAATAATTCAATTATAATATCAAAATTGTCATAAACACTACTAAAACACGAT GAAATTATAGATTACTGAAACTGACATTCAAAATTATTCGAGTCCGAATCGAAGCCCCAATGAAATTTAG GTTGAGCCGCTTATTTAGGAGGACACTTTCTTTCAAAATTGAATTAGAAAATTATGATTAAAGGTAAAGA AATAATACATCCCGATTTAATTAATGCCTCTTATTTTAAATATAATTTTATAAATATTTATGATTTATTT
Page | 22
GEN 3040
TAAAACCTTTAATATATTATTTTGAAAAAAAGTTACCTATGAGGTAACATCACATAATTAAGACGTAAGA ATAATTAAGATGAACATAGTCAGACTTCTAAGTTTATCGGTGATTTTTATGTAGCCACTTGAATGTATGA TAATTTTCTTTTATATTTTTTAAAAACACTCAATTGGTAGTCGCTTGCATTAATAATTTGACGGGTTCAT TAATTTAGAGGGATTTAATTAGTAATGGGTGTGTAGTGGATTGATTTATAAATTACACTAATAAGTTAAA TTATAACAAATAGTTGAGCGCCACGTATTTTAAAAAATATTTAAATATAAAACCGTTAAATAAGTGGTCA ACTTTGAACCAAAAGGTGGATGACAAGGGTATTTTGGACCTAATAGGTGGGTGGGAAGGGCATTTTGGAG CCAATAGGTGGATGAAGGGTAATTTTGTATCATTTTCAATACTTTAAGGGTATTTTAGGCCCTTTTCCGT AAAATAAAATGACAAGAAAATTAAATAAGTAATAGTACGACTACTAGTATGGACAAATAGAAAGACAACA CGCTACTTTCTACTAACTTTCTATTATAATTCGTGTTCTCCACAAACTTCGTTTCTAAGGTCCTGTTCTC AGTAAGCTGGTATTGCATCAAGTTTTGTCTAAGTACGTATCTCAATACTCTTTTGACCTCCTTCTTCTCT TGAAACCAACTATAATAAACCCTTCTCACCTCCGCGATGGAGCATATGTGTATAATTTTTTTTTACATAT CTGAATAATCTCAATCTCGCTTTTCATCTTGTCCTGTAATAATATTAATTTCACTGTCCAGTATATTTTC ATTCCTAATCTTATTTCTGATAGTATGTCCACACAATTATCCAAACGTCCTCACTTCTATGATCGCTATC GAAGAAAATGATCCTTATAAGAAAATTATCTCTCAAACTTCAATTTTTTATTTCTACATGTTCAAACAAT ATTCAATCATTTTAACCTCGACAAATACGTAACTCTACCACAATCCACATTGTTTATTAAAAAAAAACAT ACTTTATTCTTTAACACATATTTATAAATTCAAAATTTTATCTTAATAATATTGATATTATTGTAAAAAA TTATGTATTACATGAAGCGGAGAATACTAGTATCATGTAAAATTGTATTACTCCGTAATATATTTTTTTA ACTCCCTAATTGGAAATTATTTTCATGAATTCTTAGGCTTGTTTAGTTGGGTTCACGCAAGTGGAGAATG CAATTATTATTAAAAAAAGTAAAAATTTAAGGAATCCCATAGTGTAATTCAATAATTATATTTTGTCCAG ACAAAATTAGTATTTACAAAAAATTCCTTAAATTTGTTGTTCCGTGATACTTTAGACTCTAGTGATACAT GGTAAATGATACATGGTAAGTTTAAACTGTTGAGAAAAAAATGGGGAAAAAACGGTATAATTAATGTTGT TACTAAAACAGCTTAATTTAATGTAACAGATCATTAATCACCATAAAATCAGCACGTAATTGGATAACAT TCTCCCCTATGTATAAGGTAACAGATCATTAACATTTGGATAACATTCTCCCCTATATATTTTTCAAAGT CCTTTAGGAAATTGTTGTTAAAATTGACAGCAAACCTTATGGGATGTGTTGGTATCTTTTTAATCTTGTA CTTCAGTGGCTGCAATTTGAAAATAAAACAGCAAATTATAACGATATTGAAATATAGATACTAAATAAAA CTAATAACTTACTGATACATGGTTTCAGTTTTCAGAAATTCATACTACATGAAAAACATGTGATACATGT CTACTACATGTATCAGTGAATTAACTAAAACATTATAACATGTGATACATATCTAATGCATGTATCAGAA ACATCGACTAAACAATATACACACTGATTCTATATGTATCATCAAGTACAGTGGATGAAACATTTATCAA ACTTAGTGAATGATACATTATAACAATTTTCAAAACATGTATCACTACATCAACCAAACAACTAATACCT TACTGATTTATGAAAGTGAATATCATATATATATATATATATATATATATATACACACACACACATCATT TGAACCTTTTGTAAGGTGACCGTTCATTTTCATTTGCTTGCCTCCAAGAACAAGAAGATTACCAAATTCA TCTGCTTGCCTCCAAGAACAAGAAGATTACCAAATTCATCTGCTTTTTGGTTGTTCGTTTAATCTTTCCT CTGATTGGAATTTGTTTCTTCGGTAACTGAACTTGGGGGTTTAGGGTTTTTATTTTTGAGAGTTGTTAAG CATGTGGGTTTACGTTGAAGTTGCAATGGCGGAGTAAGTAATCTTTAAGAGAAGGAAGATGATTATGAAA AAAAAAGAACCGAAACTTGATGGAGTAAGAACAAAACGGGAGAAGTTAAGAGAAGTTGTTCTGATTTTTG AAATTTCAAAATTTTTTAATTTTGAAATTCAAAATTTCAAATTTGGGTCTTTTATTTAAAATTAATTAAA ATTAATAATTCAAAATCAAAAAACTGATTTAAAAGGTTGAGTGTTGAAATGGAGAAAATTCAAAATCCAA AAACTGATTTAAAAGGTTGAGTGTTTTAATGGAGAAAAAATAGGCATTAAAATGGATAATTGTGTATTAT AGGAGAGAGAATGTAATGTATCTATAAGCTTACACTAAAATTAAAAAAAAGGGAATTATGTAATATTTAA AAAAAGAAGAAAAAATTAGAGAATATAAACTTGTAGTTGTGTATTTAAGTTATTTTTCCTTAAAAAAATT GAAATATTTTTGGTATATTATATCATGTCTGAAATCCCAAAAGTTGGGAAAAAATCATGTCTTAAATCCC AAAAGTTGGGAATATTGGCATTTGGACCTCTAACAAAAGTACATAATTAATGTTTTTATTTTACTTTATG CAAGTGCAGAATATTATTTGATTATTATAATTAAAAAATCAAAGATTTTGGTGTAAAACTATGTTTATTA TAATTATATTCACACTTACTAGTTACTAATCCTATTTTAGCAAAATCCAAAAAAGTATACAAATTACTTT TAATCTTTTTAAATAAGCAATCCCATAAATTGGTTCTCTCCATTTTATATTAAGTTAATTTTTGAGTTGC TTCATACATTTAAGAAAAGTAAATTAAGACGTAAATTGAATCTAGTTTTCATGTTTTATCTTATTAATCA TTGTCAAATTTATAATTAAAATGACTGAATGTTAATTACTGTTAATTTCAATACTAACTAATAAAGGATA AAATTTGAAGAACACTATAAAAATAGTCTTAAAAATTAAACAATTAAGTTAATTTGAAAAATGAAAAATG AATCAAAATTAACTTAATATGGAATGAAGTGAGTAATATTATTACTCGTTCCGTCCCTAATTACTTGTCA AATTTTCCTTTTTTATTTGTTTATTTTGACAAATCAAAAAATTTAAATAATAGAAATAAAATAATAAATT CACTATGTCAAACATTATTTTTCTAACCGGTGAGTCAAATGAAAAAATAAATAAATACATAATTAGGAAC AGAGAATACAAAAAAAAAAAAGGTCTAATTTAATCTCAACCGTCAAAAAGCGCGGGAAAATTAATATCCA AAGGCCAACAAAAATGGCCTTATAAATAACACCATTCTCACTTCATTTTTTTCACAAAAATACTGCAAAA AAAAAAACCCTAGAAATCTCTGCAATGGCACCAAAGAGAAAGGCAACTGCAGGGGAGCCATTGCCAACTC GTATAACTCGAAGCCAAACCAAGAATGACAACTCGAAACCCGTTTCAGTTGACCCGGTTTCACCAAAACC AAAACGGGTCAAAAAGGGTACCGTTTCTGAGACGAAATCTAAAGTGAAGGCTCCAGTTGATTCCGGGTCG AAGAAGATTGTTGTTGTTGAGCACTGTACCCAGTGCAGGCAGTTCAAGATTCGGGCTGTGAAGGTGAAGG
Page | 23
GEN 3040
AGGAGCTGGAACATGGGGTACCCGGTTTGGAGGTTCGGGTCAACCCGGAAAAGCCAAGGAGGGGTTGTTT TGAAATCCGGGTCGATGATGAAAAGGGTGAGAAGTTTGTTAGCCTTTTGGATATGAAGAGGCCATTTGGA CCCATGAAGGCACTTAATATGGACAAGGTCATTTCTGAAATTATTGAGAAAATTAAGTGATTCATTTTAA TTTTAATTTTATTTTGCTTAATGGGGATTTAGGGTTATGTTTGGATTTTAAGGGTATGATTTTGTGTTTT GTATGACTAAAGTATTTGATATTTTCAGGTCATTTGTAGCCCTTCGAAAAATATGGAAACAATCTGTTGT GAATTTTGCCTTTTGTTTTCCCAAAACTATGGTTAAAATGAAATGATTAGTGTGGTTGAAACTCTATTAT ATTCGAGATTTGAAATTTATTGATTTTGAATGTCTTTGGATCAATGTCCAATTATGATTGTGTTCACAAC CAAAATTTTATGATGTTATTGGATGATTAATTTATTATATCAACAAGGTTTGTGTTAGGTTAATTGGATT TTGTGAACTTTAGTATATAGGAAATGATTTTTTCATTAATGAATAAACTTGTTAGGTTAACCAAGCCATG GTTGCACAAGCATCAATTGTGTGAGCATGGCGTGTTGGCATTGGCGGAGCCACCTTGGTACAAGGGGATC ATCCGAACAAACATCTTTCGACGAAAAATTATACTATTTATATATGGTTAAAATAATATTTTATCTATAT ATTCCTTTGACTACTCCGTGTGTCTTGTTTTTATAAATTTTGAACTCCTTTATTGAAAATCATGATTCCG CCCGGTGTGTGTTGGCATTGGACTTTTGAGAAGTATTTGGAAGATGAGCAATGTGAGAGAGAATATATTC TAAGAGTATCAAATAACTTTAGCAAGTACTTGTGGTGGTGAATTCAAGGTGTGTGCCACTCACAAGTTCT ATAGCCATGAGTGTGGTGTGTTGATATTGAGCTTGAGCTTTTGAGAAGTAGCATGGGTGGCACTCATATT TTTCCTGAGTTATAAGTGTAGTATATTGATGTGGGGCATTTAAGAAGTATTGATAAAGGATCAATCTGAG AAGTAGGGTTGTTCATGGTTATGGTTAACGATCAAATCAAACCGCAATTCGAATCAAATCGATATCTGGT TTAGTTTAGTTAGGTTTGATTTTTAAATTTTGGAAACCGATACTATTTGGTTTGGTTTTGGTTTTACTAA AAAACAACTGCAAAAATAACCAAATCGAACTGATAAATTAGATATATAAATTTTATAATTATTTATATAT TATTCATAAATAAATTAAATATATTGTTTAATTTTAATTAACTTAAGGCTTTAACTTTACCATTTTTTCA AGTCTAACACTTTAGCCAAGGTATAACAATCTCAAATCCAAGTCCATCAAAATCCGTTTTAGTCTTTACT TATTGTACCTCACATAAAATAGTCACAGTTGTCCTTTTTGCATCTAGGCCTACACCAGTTTATCTTGCGG CCTCATCATATTTGTATTAAATACGTGTATATCATGTGAACTTGAATATATGCCTTATAAGCAATTGGGG ATCTCTACGAGGAGCAATTACAACTCTAAGTTCTAATACTTTGCACCACTTCTTTGTTTATATAAAAGTT GGAGATATTGTTACCATTTGACAGAAAGGGCAAGTATAGAAAAATATACGACTCGGAATTTTCATAAGCA ACCTGGATTTGATCATTTCCAATTGAAGAATTGTAATTTTCTTTCTCAGCTACATCTTTACATTAGTTTT TATTCCATACAATCTAATTTTTATAAACAAAATTTGAAATAAATATTGTTGTACAAATATTACGCCTCAT AAAACTTACCTTTTTAGGTCGCAGATGAAACCTAATATCTCAAATTTACAAAGAGATGACATTCCATTGA ACATTAGTAGGCATTATGAGTTTGGAGATGCACTCACAAGAAAGAAGCTAGTTGTGGGCTTCGATAGCCT ACTAAAATGATACGAGTGATACACAGTAAATCTCAAAAATTGACTCGTGGATAATGGTGATGGTTACGTG AAACTTAGTTCAAAGGGGGTGATTGTCAGGTTTGCAAACAAAATTTCATTGTTCTACATGAATAGCTAAA AAGATTGAATAACTCATATAAAGTGCATAATCTCTTATTAGTGTGAGGTTGACTGGGGAAAAACCATTTG AGTTTGGCCCAAAGTAAACAATATAACACCATGTAAAAGTATCTTTGGACTGGCTAAGCGCAACGTATAA TTTAACTTTTTTTTACGTTGAAAATACAATAGTAAGATAAATAAATGATATAATGTAGGAAGAGTATTTC AAAGATTACAAATAATATCAGCAATAAAATCGTAAAATAGTTTATAATTAAAAAATGTTTATTACGTGTA AAATCTTAAGTTTAGATAATCTTATGACTTTTGACTTATAACATTTTAATATTTATCAAAATAGTTTATA AAATTAGTCAAAACACATTTATAAGAAATGACTTACTTACTAGTTTACCTTATCTGCATCATCAAAAAGA TGTCCAAAAAAATTATTTCAGATTCTCCTAAATTTATTCCCTTTTTAAAGAAAGCCAAAATGTAGAAAGT TAATGGTAACATTACTATTTTTAATTGCACCATTTGAATAAATACTTCCCCATCTTCTACTAATATACTA GTTTAATTTCAGATTATTTGCATTTTGTTACAAAAATCATCTCCAAAGTCATGTCATATCAGGTAACTTT TCAAAATTCATTTTCGTTAGGTTTGAATTTTTTACTCGTATCTATTGATGATGGGAGCTGACAGATATTC TATAGAATTAGTTAAGTTCTGTAAAAAGTTAGATCCGAATAAACAAAAAGATTGCTTTGAATTTTTGTTT GCTAGTATAAATAATGTTGTTTATGACTTTTGAATTTTCTTGTGTGTTTCCATGAAATTGAGGTGATCAT TTATATATATTTCTATTCCAATTCTTTGTTTGGGTCATTTATATATATTTCTAATCCAAAAGTTTGCTTA ATTATTTCTCAAATTTATGTAGAAATCATTGATGCATCTGAGAGCTAAAGTACAAATTGTGGTTGCAATT TCACTATTGTTGAGCTATGCCAAAATTGAAGCAAGACAAAGCCTATCTATGGTTGAAGATTTGGAATTGG AGAGGCAATTGAAGCTTATAAATAAGCCAGCTATCAAAACGATTAAGGTACAGTCTCGATTTTTTATATA AAGATTAAAAGAAACATCTTTTGTCTTACGTGATGGAAAAGTAATAATGATCACAGTATAAATATTAAAT CTAATAGTTTGTACTACATCTATTTTTTTTCTATTAAAAAAAATTGATTGGAGACTAATTACTAGTACTA TTTGATAAGCAGGGGAAATATGGAGAATATATGATTGTGTAGACTTTTACAAACAACCTGCATTCGACAA TCCTCTATTGAAGAATCACGAATTTCATCCTGAGGTATATCTCTACCTTAGTTTCAAGTTCGTACAATCA AGTTTTTATAATCAAAATTTGAACCAAATTTAAGACACAATTTCTTTACCTCTTAAGATAAGAGTAAGGT CTGTGTACACTCTACCTTCTACAGATTCTACTTGAGAAAATTCACTGAAAATATGATGTTGGATACAGAT GAAACCTACCTTGCCCAATTTACAAGGAGATGACACTTCATCAAACATTAATAGCCCTTTTACCATAGGA TTAAAAGACGGAGGTTGTCCTACTGGAACAGTTCCTATCAGAAGAATTACTAAAGACGATCTAATACGAC AAAGATTCTTATCACAAATTAGAGGCGCGGATGAATCCCCTTATGGTGATGTGAGCTTGCTGAATTTCTA CAACTTCTTTTCGAATTTCATAATTCAAATTCTTTTATCATATTTTTCTTCAGGATATGGAGTTCAGTGT
Page | 24
GEN 3040
TTTTGATGGAACCAACTCAAGTATACCTTTTAAAGGAGGACGCAAGGTTTATATACTTTTATTATTCCAT ATTCTATCCACAAAAAACTAATTTTTTCGATATTTTAACACATTATATCCACCCACCTCCCAAAAATAGC CAACATATGTATAATATATGTATATACATGTATAGCAATGTATAATTAGCTTAAAAAATGTTTTGTTTTT GCTTTGTTTCAGTATGCAACAGTGCAAATACCATACAACTCAACAAACAAAATAACAGGAGCTGGGGCAA TTATTAGCTTACATAATCCTCAAAATCTTAGTGGACATCAATTTAGTGCTGGTCGTATTAAGGTTCAAAT TGGAATCGAAAGTATACAAGTCGGTTGGATAGTAAGTTTTTTGTCTACGACTCGAGTAATTATATGTGTG ATTGCATCTACTGACTCTAAATCCTGCATCAGACTAAATATGCTAATCGAAGTTACCTAATAAATTTATA TTAGGTGAATCCACATGTCTATGGAGACACTCATACGCGATTATACATATATTTAAAGGTAGTTTTCTTT AACTTCTTAAGAATTAAAACTAATTAAGATATAATTTTCTTGAAAGTCGATGTATTTATTAATAAATTTT TTTATGATCGCAGACAGAAAAATTAGCATGTTTTAATACACGATGTCCTGGATTTATTCATATAAATACT GCAATACCTTTAGATGGAGATCTTCCTGCCTCTACTTATGGTGGACCTATTTATGATGTACCAATGTACA TTGCTCGTGTAAGTGTGCATCTTCCCCTTTTTTTTATAAGTTTTTCACTCGATATCTGATATTTGTTTAA GGATCTTAATTAATTGAATTTACGTTTGAGTAGAATTTATTAAAAAGAAACTTTTTAAACTATTGCATAT AGATTATTTTTTTGAGCGAGTGGTAAATTTTATGAGAAAACGTTAGACTAGTGTCGAAGGTTTGTAGACA TACACTACACAACAATATAATGTTTTGTGAATTCCAAGAGCTATATTTGCACAACTTTCAAAGACAAACA AAATCTAAAATTGTGGCATGGAAAGAACATTCGTGTAAATCTTCCTTCTTCTTCTGTTGTTCATATTCTG AGTTCGAATTTAAAATACTTTAATTAAAGGTGAAATAATTTTTTTCATCAGAAAACGTTTTATAATCCAA TTATGATAATTTTTTTAAAAAATAATGAATATGTATATTCATGTAACATGTTATAACATGTTACTTATCA TTTATTCTATATTACTAGTGAATGTTTATTATATACAATAACATGTAATATCTTGTAAGTGATTTGATTA GATAAATAATACACACTATCAATCCATAAACTTAACCTTCTTTTATTATTATTATACAGTATCTGACTTT TTTTTTCCTTTTACCATTTATAGGATATGTCAAATGGAAATTGGTGGTTTAAATTTGGAACAAACTATAC ATCAGTTGGATTTTGGCCATCAAAGATATTTACAAAGTTAAATGAATTTGCAACAAGTGTAGAATATGGA GGAATCGTATATAGTCCACCAGGTGTACCAGAACCTTCTATGGGCGGTGGCTATTTTCCAGTTGGAGATT TGAACAAAGATGGATATTGTAAGAATAGTACCTATTTAACAGATAAAAATGAAACTAAAAAATCTTTAGA TGATATTGAAGTGAAATTATATGCAAATAGTCCAAATTTGTATAGAGTTGCTGATTTTCCAAACTCTGGG GTTGAATCTGGTAATTTAGTCCTATATGGAGGACCAGGTGAACATATGTATAAGAAACATAGAGATATTT GATATATAGATGCGGTGTTTTAAGATATTTCGATATTATATTTATGAATTATATATGGTTTGTTCATGCA GTGGTTCTTGGTATATAAGTAAGTAGAATGTGTCAACTAGTGGTGCATTTTAAAGCCGTTCAAGTCGAGG TTTAGAACGGACAATATCACTAGGTGGCTGGACTGTTACAAAATCATTAGATTTTGTTCAAATCTCGTAT TTATATTTTTAAAAATCAACTTAATTCTATAAATATTTAATTATATGTCCAATTCTTTTGTCTGATACGA ACTTACATGATCATATTAGAAACTCATAAACTTTAAATTTTGTATCGAACATTAATGAAACTTTTAACTT TTTTCTTCTTCTTTTTGGTTATAATATAGGACATGTCTAATGGAAATTGGTGGTTTAAAAAGGGACCAAA CTATACATTAGTTGGAATTTGTCCATCAAAGATACATTAGTTTGATAGTTTAAAATAAAATTTTAGATAT TCAAAAGCTATACAGAAAGTATTATGAATTATAATTTTTTTTATATAAATATAATATATATATATATATA TATATATATATATTAGTCAATTATTTGACTTAAAGAAGATCCTTTTATAAGAAATCTAAAATAATAATAT AGAAAATTAATTGTAACATTTGACTTTTACACTATTCAAATAAATACTCTCTCGTCTTCTACTTAGAGTC TGTTTGACTCAACTTAAAAGCTGGTCAAACTGACTTAAAAGTTGGTTTTTGACTTATTTAACTGTTTGGC AATACTCAAAATAACTTATTTTAAGTTTAAAAAAAACTTATTTTAAGCTAAAAGTTAAAAGCTGGGGTAG AGGTGCTTTTTTTTTAAGCTTATAAGCTGTTTTAAGTTGACCACATTTTTATCTTTTTGCCCTTAATATT TTTATACAATCTCCAAATTACCCATATAACCCTAACATCTCTTTCTTCCATTTTTCCCTTTTCACGTTTG GCATAACAACTTCACCACTATTATCCAAACACATAACTGCTTATTTTAAAAATAAGTTTCAACACTTTCA AAAGTACTTTTTTAAAGCTGCTTTTATTAAGCCCATCCAAACTGGCCTTTAGTATAAATGTATAAGTCAA TTATTTGACTTTAAGAAAATCCTTTTATAAGAAATCTAAAATAATAATATGGAAAATTAATTGTAACATT TGACTTTTACACTATTCAAATAAATACTCTCTCATCTTCTACTTAGTATAAATGTATAAGTCAATTATTT GACTTTAAGAAAATCCTTTTATAAGAAATCTAAAATAATAATATAGAAAATTAATTGTAACATTTGACTT TTACACTATTCAAATAAATACTCTCTCATCTTCTACTTAGTATAAATGTATATATGTTTGTTTAAGTTCA CATGTATTTTTATTTTGCATCAAATTTCAACACCATCGTCATGTCAAATCAGGTAAGTTTTCAGATATTT ATTTTCTTAAGCTTTTTGAAATTTCATATCGCTTAATTTTATCATACGTTATATTTTTGTTCATTCATAT CTTTGATAACAGTAAATCGTTTCGTGTTTAACCTTTTGTCATTGAGGAATGCTTTACCATGAAATGGATC TACATGTGCCCAATTTTTTCGGGAATGAAAATGTAAATTTACCCTTTACCTTTAGACTGTAGTATAAAAT TATTCTCTAGTTGAAATTTCGACAGAGGTTAAGGGTAAACAAGACTTTTTCCCTAACAACAGAGGATTTA TATTATATAAATTAGACCAAAAATTTAACACAATGTTGATAAATTACATCTCAAATTGTAGATATTACAA ATTGTTGTTGCAATTTCACTATTGTTGAGCTATGCCAAAATTGAAGCAAGACAAAGCCTATCTGAGGTTG AAGATTTAGAATTGGAGAGGCAATTAAAGCTTATAAATAAGCCAGCTATCAAGACTATAAAGGTGATATA TATCATAATATTTGAAGCATATCAAAATTATTTATCAAAACGTAGGAAAAAATAAATAAATTCACTTTTT TTGGTTGATTTGTAGACCAATGATGGAGATATATACGATTGCATAGATTTTTATAAGCAACCTACTTTTG ATCATCCACTATTACAGAATCATGATTTTCATTCCGAGGTGTGTTTGTATTATATACTTCTATATTCAAT
Page | 25
GEN 3040
TTTAATTAAATTTTATGACAAACATTGATGTATTTTAAATTTGACGTAGATGAAACCTACTCTATCCAAA TCAAGAATAGACGCAAAGGCTTTGAGTTTCGAAAAACCTTCAAAGATAGGGTTGAAAGGCGGTGGATGTC CTAGTGGAACAGTTCCTATTAAAAGAGTTACAAAAGAGGATCTCATTCGACATCAACATATGCAAGACAT GAAAAAAAATCTTAATGATCATGTAAGTTCACATATTATATTTATAATGCCATATCGATTTTTTATCGAA TTCATCATATAAATATGTAATACTTTATATCGCCGATATATAAAATATGATTTATTCTTTAATATAATAC CCCACCCCACCCCCCTCAATTTTTTCCTAGTTTTGAATCTCTAACATAAGGTGTACTTGTACTTCATGTA GGGGAACAACATAACCAATTCAAAGGCCTCAATTTCCCTAGATGAAATACATCCTTTCCAGGTGTGTATT GAACCCCACCACCCCCCACCCCCCCACATCCTTTTTTTTTTCAAAATAGATTCGGACACTTTTGAACTTT ATTCAATGCATATCTAAACTTGATGTTTGCCTAATTAAACCCGAACATGACAATTTGATAACCTCGTCAA GTTCAGGATAATTAGACCAAAGTAAAGTTCAGATGTACATCGAATAAAAATCGTGAAAAGTGTCCACTTT GAATCATTTGCATATAAATTATCTTACTTTGGGATTATTATTTTATTATTGATTTTAGTATGCAGAAACA GAAATTCCAGGTGCTGATATAAATGTACTTCGTGGAGCTGGCATGATTACTACTATACATGCACCAATGA ATGTTCAAAAAGATCAGTTTAGTGGAGCTCGTGTGAGGCTTGAAAATGGACTTAGTGATGCCATTGAAGT TGGTTGGATCGTAAGCTCACTTCTACTTCTCATTTAAAAAAAAATTTAACTTTTATGGACTAACAGTATT CGAAAAGAATACATATATCATCGACGATTATCTGTTGTTACAAGTCAAACTTATAAACTTAATCAGTTAT ACACGCACGCTTATATATATCATTTTATGCAGTGACAGTGTTTAAAAATAAATACATATATCTTCGACGG ATATTTTTTGTAATAACTCATCTTATAAACCTGATGGTGTACTAGAAATTTGTATATATCATCAACTGTT ATACATATTATCATTTCATACACTGACAGTCTTTAAAAAGAATACATATATCATCAACAGTTGTATGTTG TTACAAGTTTTCTTATGGTGTACTAGAAATTTGTATATCATAGATTGTTACACATATATCATTTTATGCA TTGACAATGTTCGAAAAGAATACATTTATCATTGACGATTATCTGTTGTTTCAACTCATTATTATAAACC TAATGGTGTAGAAATTTGTATTTCATCTACAGTTATACATATATCACTTCATATACTGAGAGGTTTGAAA AGAGTATATATATCATCGACAGTTATACGTTGTTACAAATTATCTTATAAACCTAATATGGTTTAGAAAT ATCGAGAAAATTACTAAATATCTTTTTTTCTAAGTTGAAAAAAAATACTTGTTATTAATATTATTGTTAT GCTAAACTTAATGAAGGTGCATCCTACTCTTAATGGAGACAATACGCCAAGACTTTATGCAAGATTTGAG GTAATTATGAACAAAACTTAATTACTTTGATCAATTATTTAAAACTTTTCTTAGAGACTATAGTGTTTAC TAATTATAATGAAATGCTTTTTTTTTAATGTATTATTATAGGTTGGAAGTGCTGGTTGTTTTAATACATT ATGTAGTGGCTTTGTCCTAGTAAACACAGATATACCTTTAGGAATGCCACTTGTACCTTCAAGAATTGGT GGACCTATTAATTCACAAATAATGTATCTTGAACAGGTAATTCATATTTATTTGTTCTGTTACGTTAGTG AAATACGTTTTAAAATTTCTACAAAACTTGACGTTGATAAACGCTATTTACGAAATGTTCCTTTTATGTA TCTTACTATATGTCAATATAAACTTATATAGGATGTAGCAAATGGAAATTGGTGGGTTATGCTAGGAGAG GATTACAAACAAGTGGGATTTTGGCCTAAAAGTATTTTTACAACATTACAAGCTTATGCAACAGGTGCTA AATATGGAGGTATAACATATAGTGCACAGGGATTACAATTCCCTCCAATGGGTAGTGGACTTTTTCCAAA GAAAAATTTGTTAGAAAATGCATATTTTAGAAAGTGCACTTTTTTGTGTTATGTGAATGATGAAATGGTA ACATACTCTTTAGATCATATTGGCACATATCCATTTCAGAGTAACACAACTATATATACAGTTCAAGATT TTATTGAGCAAGGTGATGTACTTGGTCATTTGATTGTTTATGGAGGACCTGGTGGATAAACATTGATGTA ATGTTGACATTATGAATTAAAAAATATTTGGACTTTTATCTGTCATATAATTTGTATTTTGCATTCATAT TAAGAAAATAATGATTATTTTATGATATTAGGCAGGGGCGGCTCAACGTTATTGGAGGCCTAAAGCAAAA TTTCAAGTCGCGACCTAAAATATAAATTTTTTTGAAAAATAACTTGCACTATTTAGATGAAAATTATTAC TACTTAATGTTGTTTTTTCAGTTATTAGTTTTAGGTAAGATTTTATCAACTCAACATTGAAAAACATCCT TTAAGTGAGACAATTATTATATGTATTGTTAACATAATGATACAAGTAATAAATTTATAAATATTAAATA ATGATTAGAATTAGAATCATAAATTTAACTCAAAAAAGTTGATGATTTGGTATACCTCATTTTAATATGC TTAGAGTAATGCTTGAAACCAAATTTTAAAATAAATAAAGAAGAATTTATAATTCACTTTGTTCAACATT TTTCATTGTTAATTCTTATTTTTAAGAAAGTACAAAAGATGTTAAAGTGGAAAAAATAATTTATTTTTAA CAAAATTATACTTTTTATCATAAATAATTTATTTATCTATAAAAATTTTAATACATAATTTATTCTTGAC AAAAATTTGAGGCCCCCGAAATTTGAGAGTCTAAGGCAAAGGCTTTATTTTTGAAAGCATAGAGTCGGCC CTAATATTAGGGGTTCATTCGAACTTCCTTCGAGGAAAAATCATATAACATATACATGGTTAAAATCACT TTTTAGGTATATATAAAAGATGTTGAACCCCCTTCGACTACTTTATGTGTCTATTTTTTCTGATTTTGAA CCCCTTGTCGAAAATTTTGACTCTGCTACGATATAGTATTGTCTATTAATTGATGTATAGTTTAATGTTA AGGGTAAAAAGTGTAGAAAAAAAATATCTTTTTTTTTAATATGTTAAAAATCACAATAACTAAAATTGAA AGAAAAAAAATTGAATAGTGAATAAGTAAAAATGAATGAAAGGATCGAATATATTGAAGTTTTTTGTATT ACTTTCCATACTTTTAAAAAAAAATTAAAAAAATCTCCACACATTGAAGTTTTAAGGGAACATTATAGGA TATTTTCTTGATATTAATTGGGTAATAGTTCTTGTGTGAATTTTAAGTGTTAAATGTTGTTGCTCAGTGA AGTGTAGGGAGTAGTCGTCTTGTTATTGATATGTATGAACATGTCAGAATTCGATAAATAAATAGAAAAT AAATAATTTTTAAAATTGCACCGTAAAAAAATCAAAACAATTAGTGCCATCAAGACTTTTTGAATTATTA GAAGTTTATTATTTCAATGAATATAAGTTTCATGGGAACTTTGGAGGAGATTTTCTTGACCTTAATTTTG TGGAATAATTAAAAGTTTATTATTATATTTTCAATGAATTTATGTGCCCTCTAGAAACCATATCATTTAA TAAGTTGATTATCGACTAGGTCCAAAGAGGCCTACGTCATGTTAGAAACAAAAGTCCTCTTGAATTAATT
Page | 26
GEN 3040
ATTTAATAATTAAATGTTTTGAAAATTATGAGAAAAAATATTTAGTCTAATCATTTTGAAATAGAAAACG TATAATATGATTAATTTGAATTTAAATTTATTAGAACATCATCTATTAGTAATTTTATTTATTTTTCAAT TGTATATAAAATAAAAATTAAAAAATCTTATCTATTCAATTATACTATTGATAATACTTAGAAAATTGAC CCAAAAGAAAACGACACTATGATCGATCTTATCATGAAGATTTCTTATTTTCTTAAATACGCAAAATATC ATGATAAGACATTCATAAATCCAATATAATTTTATTCTTTAAATTCAAAAAAAAAAAGTTCATAATTTCA AATAAAGAATTTAATTGGTATATGTTACCGCATTTCATATTCTTTATTTCCCTAAACATTTATATTTAAG ATCTCATAATTTTTAATTTGCATAGTTAATTACTATAAGCTACACTGTTTTGATTCTTAATCATAATTAA CCACAACTTTATAATGCATATTGTATTCTTTTTAATTATTTGATAACTATTTAAATTTATGAAACTTTTA TACTTTTTTAACGTGGAACGTGAAACACTAAAAGATAATAGAGATAAAAAGAAAAAAAATGTTTGATTGT TCATAATATTATTTTATTTCTTCATTTACTTGTTTTAATTTTTTTTTTCATATTTAAATTTAACATCAAA TTACTTTTAAAAAACGTATAAAATAGACAATTAAAAATAAACAAACAAAAGAACGGCAAGAACATGAGAC AGTTGCTGGGTTTGCACTTGCACCACGTGCCTATAAGGATGGGCTCCTGAAAAAACTCTCGTTAAAGTCC AACCAAACAATTAATATTAACACGACGATAAAATTTATTCGTATCAGGTGTTTAATTATTTATATAAATA TAAATATAAATGTTAAAAAAGATATTGGGTTGCTCCTCTTTTAGTTAGAGGTTTAAGATTTCAGCTTTGC ATGAAAAAATTTTAATTTTTTTTTAATAAAACTATATATAACTTAAAATTTAAAATAATCAAACTCTAAT ACGAATATGGAACCGAAAGAGTTTGTTTTTACCAAATAAATGTCTACACAAACCCACCAAATTCTTGTCA GCTCTTCATAACCTCCAAGCAACATCTTTTTTATTCACACTGCTATTCCCATCTTTCATAAATGACTATT TTACCCTTTTTAATCTTTTCTACCAATACTAAAATTAATATATAAATTTTTTATGGACGTTTCTATTAGA AATATGAAATTTTCAATGAAAATAATTGTCAAAAACGTTTTTGATAAGTATTAAAAAATTTTATTTCATT ATTATTCTAAAAATCAACTATATCTTGTTTAAAGTTTCAATTTTTCTCATATGTTCTAAATATGTGAGAT GTACCTTTTAGTTGATCTGTACAACGTGAAATCTAATTAGTCGAGTAAGTTGACTTCGAATATCATATGG TAAAACAAAAAGAAACTTCCCATAAACTACTTGAGAATCATAAAAAAAAAGGAAATATTATGAGGGTATA TATGTCATTTTAAACTAGACAATATTAGTTTTTTCTGGTTCTTATCTGCACCACAAGTTCTTTAAATTAA GACACATATTAATATAAAGACTTATCACCATTCAACAAAAGTTCTAAAAAAAAACTCAAAATTAGACCAA CCTAATTATTTCCCTCTCTGTCCATATTTTCCTAATATTTCTTCTTCATATAATCATCATGAAAATTCAA TATCCTTCTTTTAAATTTCTGCTAATATTAGTAATTATACTCTTTGTTAGTCAATTATCAAATTGTAGAA AAATTCAAGAAATCACTCCAACAAGCATAGAACAAAGATTGAATTCAAGAATTTCATGGCATAACCTAGC ACCATCTCCAAAAAAATCAAGAAATGAAGATATAGACAAGAGTTATAGAGTATCTCATCGAATCGTTCCC GGAGGACCAAATCCTCTTCACAATTGATCAACTTACGTTCATCTCCAACATTGATAGCTCTGTTCATCGA GACTTAGCATGTAAGAAAGAAAATTATTAAACTTTTTTTCATCTTTATTGGGATTTGAGTGCTAGCCTTT TAAGGTGTTTATCGACTTTGTTGATTGTTAGGTCACAATCTTAGGTGCCCTTTTGTGATCAATTGATGTT GACTTCTTCACATTTCACCAAATTCCCAAGTTCTTTTTGTAGCTAAGTTTGATATTATACAATTTTTTTC CATAATCTTTGTATTGTACATGGAAATCAAAGACGTATACTAGGAAAATCATGATATATTACTTTAAATT GATTCAATTACATTTTATCTTCTCCACTTTCTTTCTCTTGTTTTTTAAGTTTTTTAATTGCATACAAAAC GTCAAAATCAACATAACTTATAAAGTTATTGTCATGTGATCAGAAGGTTTCACGTTTGAGCGTTGAAAAC AATTTCTTATAAATTGTAAATGATTCTTATAATTTGACTTCACAAATAGCGAGAACTAAACAATTCTTAT AAATTGTAAATGATTCTTATAATTTGACTTCACAAATAGCGAGAACTTAACATATTGACCGATTTACGAA TAGTAGTAGTTGAAGAGCTACATCATTGCTCATTAAATTTCAAATATTTAAATTATGCAAACAAAAGTAT AGTGAGATAAAAGTGTGAAAACATACCAAAAATTAAACCAGTTGTTGAATAGAACTACTAACCAATCAAT ACTTAGATCAGCCACCGACTTATTACCAAGTGTTTATTAAAAAAATATTTTTTTTTAAAATGTGAAATAT ATTTTTTTAGAACAAAAAATTGCAAGTGATGTAAGAAAAAGTCAACTCATAGACAATTAAGTAGGTAGGC CGTGGACAAATTAAGTAGCAAATTATTGTTTATTAGTCTCTATTCAAAGCATAGTGCTTAATTTAATAAT GTTTAAGTAGTACAAGCAATTTTATATTGGTGGGCAAAACCAGCTAGTTTTCTCATGGATATAAAGACTA AAGAGTACTTATAAAATAATATAATCCACATTATATTAAATTATAGATATTTAATTTTTATAAAGAAGAG AAGGAAAAAAAAACGTTAAGAGTTAAAGATCGTAAACAAATATTGCGTAATTTAATATTTTGGTGTCCTA GACATAAGAAAACGATATCGATCCATATTTTAAGAATCAATAAAATAAGTAAAATCATGAAAATTAAAGT TTAAATTTGAATTGTAAAAAAAATGTATGTACGAAATTGCTGAGGACATATAAATTGACTGAATGCGATG ATTATAAAATATTAAAAAGATTATATAGATATTAAATATAATAGTTGAGTTGTACACAAATTAATTAGGA TATTATTATTATTATTAAAGAAAAAAAGAACAGTATTAGTGCATGGTTACTTGATGAGTTGTTAATCCCC CTCCCTATAGGCTATATATATATAATTTATTATTTTGGCATAGAAAAAAACAACTTTTTTTTTGGAAGTA TTTGGTGTCGACTTCAATAATATGGACAATTAATTAATTGTGATGTGAAGTGATTAGTAGTTTAATATGT ATTTTGATAAGTAACTTCAAAGAGTACTTAGTCTTCCATGAGAAACACACAAATTAAATAATACAATATC ATTATCATGCAAGCGTCTACAAAATGGACTTCTTCGACCCTATCAAATTAAACTAATTCATTATAACAAG TTATTATTTTTATCGAGCCTTAGGGTGTGTTCTGAAAAATATTTTTTTTCAAAAAACGTTTTCTAATCTT TTCATATATCCTGTTGGTCAATATATATATATATATATATATATATATATATATATATAACACAAATTTC TTGAAAGCTAGAAAACATATAAGAAGTAATTAAATAAATTAACTAACCAAATTTCATCGGTGGCATTTCA AATTCATTGTCTCATTTTCATCCATCCGATGCTTCCAACCCCTCAACCCTTGACGACCATTCCACCTCTC
Page | 27
GEN 3040
GAATTTCGTTATTCTAAGTTATAAATGCTTTTATAAAATAATATAAAAAGTCACTCACTTATCAAATACT AGCAAATAAATAATTATAAAAAACTCATTTATGTTTTTTAAGAAAATGTGGAAAAGCATTTTCTACCACC TTTAGTCATAATATAATTTGGCATGGAAGAATTATAGAATATGAGACTAATGTATCTCAATTTGCTTTCC CACAAAATGTTCAAGCTAAGCAAGAAACTGAAGTTTAAAGTATATTATATAATTAATTAAAGTTCCAATA TTATTATTGACTTTATTCACAATACATATTTCTTCAAAGATAGACCTAACACGTAAGCTACTAAAAGTCA AGTCATGTTTGCTGACTAGATAAAATAGAAGTTAAGGTTTGAGAGATTTTTTGTTCGAGCCTTCGAGCAG TTATCTAGACATCATTTTATCCTTAAATATTAAATTTTTTTATGCAAATTTAAGTTTAATCAAACTCTAA TGTAGGTACTACGTACTAAATATTAATAAAAAATCAAACTAAAAGAGTTCAAGTTGCTGAAAAAAATGAA ATTGAAGATTAGATTTGATTTTTCTTATATCAAATCAATATTTTATTTTAATAATCAAAAATTGAATTGA GAATATATATCAGGTATAATATAACTTTCGTAAGCTAAGTCATGATAAACATATGTATGAAAGACGTATT TTGCATTCATATGATATATGTAAACAAGTTTAATTGGTACAGTAATCTAAATCTCACTTCGTGAATTTCA TTTATATGTTGTTGTAATAGACAAACTGATTGTTGAAAAAAGATAAAGACAATAAGAAATAAAAATATAT AGAGAAAGTATTAACAAGTAGCACCAGTGGTCTAGTGGTAGAATAGTACCTGCCACGGCACAGACCCGGG TTCGATTCCCGGCTGGTGCATTTCAGAGCAATGATGTTTACTTGCACAAATTGCGAACGATTGGGTCGTC ACCTACATCTGATTTATAAGGCAGATAATGTGCATCTGAGCTCTATTTTTTTTATTCGTTTTCGTTTTTC ACCTGAATTTAATATATATTGGAGATTGATTATATTTAGAAGCGCTCCGTTTTTCTTTTCCATCCCAGCC TGAGTTGCTTCGTTTTTATTTCCACCCCACTGAGTTGCTTCCTCATCTGTGGTCATATATTATATTTGAT TGTTTGTAGAAAAAAAAAAGAGTTTAAATTTTATGTGTTTTAAAAAAACTAATAAGAAAAGTGAATAATG TTTTGAACTCCAATTTATTTAATTCAACCACAACTCCAAAGTTAATGTATACTGAAATCTTCTTTAAAAA GTCAACTTTTTTTGTTGTTGTTATGATAAAATGAGGCAAAAATATAAATGAATTTAGAATTTTATGGCAC AATAAACCTACAACTATAATATATATATATACATTATAGTAATATGAAAAATCAGATTCTACAAAAAGAA TTTCTTCTTCTAACAGTGTCATTCGACAGTTTATATGCACTTCGATTATACATTATACACACTTCGACTA TATATGCATGTAAATATTTTGTTTTTTGCTAATTGATGTCCTTTACTGGTCCCTATAAGACAACTAGTGT TACGATACCCCACTGATGCACAAGAGCAGTGATGTTTACAAATTGTGAACGATTGGGTCGTCACTTACAT TTGATTTATAAGATGAGATAATGTGTATCTGAGCTTTACTTTTTTGCTCCTTTTTATCTTGGTGAAAATT TATTTTAATATATATATAATTTTTGGATAAAAGTATTGAATTTCCGTAAATCTGTAACTTTCACGTGTTT TCTTTTACCTAACAATAATAACCATCTCAGTACTAAAACGATAGTGAAGTGAAGTTGTTCTATCATAAAA TTAAGAATCGATAAAAGAGTATATTGTGTTATTCTCAAATTTGAAAATAACAATGTACGTTATTATCCAT TTTTCAGTTTTTATCACAACCTTTCAAATTATGTTGTTCAAACTTTTCAAAATATGTCAGACAAGTACAT ATTGAATTCTTCAAAAATATCATATTTTTAAAGAATTAACACGAATACAACAATAGCTTTCATTGTTAAA TTTGACTATTTAAACAATATAATTTTTAAGTACATCATAATAATCATTACGTATAAAATAACTTACTCAA AATCAACATGAGAGTGAAGGCTTGGTAGATTATCAACTATAAACTAAGGGCCCGTTTGGCCATAAATTTT CCAAATAATATTTGGGAAAAATTTGGCAAATAATGTTTGTCCATACAATTTGTCATTATTTGGCAAATAT TTTTGGTAAATATTCCAAATTTTCAAATACTAGTTTTTTTTAGTATTTGGGCCAAATATTATTATTTGGG ATATTTTAAAAATTAAAATTTTACCCCAATCTTTTATCTTTTACAAAAACACCCTCTCTAGTATTTGTTT GCGTTGTATTACATCATTTTTTACGTGAACACCAAAATAGTGATGAAATATTTAGTGAATATTAAATAAT GATATGATTGTTGATGAAAATTATTAAAAATTGGCTTTAAGTAACAAAATCATGTACTTTATCTACTTCA CGATGTATGGAATAATTCTTGTTATACTCACTCCAAATTATTACATTGCTTCAGTGTCATGGACATTATT TGTTATTGTTGTAACTAACATTCAATTGACTTGTAATACAAACTTTTAGTTAGTTTTGATAGTTTTTAAA ACTTGTGTGTATAAATCATATTTTTCTAAAAAGGTGAAATATATTTCCCAAATTTTATGGCCAAACACAT GGTGAAATTTCACCCAAATTTTCACTCAAATAATATTTGCCAAAAATATTTGGAAATCTATGACCAAACG CTAGCTAAATATGTCCACTCAAAGATCAGCAAAAAAAGAGTATATAATAAGTTTTAAATTGAATATTTGT GATTAAAGACACTTTTATAAGGTGTTAGCATCCTCATTGTAAGAAAAATTTTGCGCGAATAATTCATTTT TTACTGAAATAATTTTATCACTAAGAAGATTTTTAATCGTCTTTATGATTTACGATACTTATTCCTTATA CAATTATTTGAGAAATGATTCTTTCCCTCCGTGATCGATATTAATTTTGCTTTTGTTATAGATGTAACAG TCTATAAACTTTTTAAAATTTTTATCAATTCTATTTTATTTATTTGGCAAGAAGCAATAATTATTCTTGA TATGGTTTAATTTCAAAGAGGAAAATTGATAAATTGAATGTGAATCTTCTATGTTTAACTTTAGGATCAT CTTATTTATTGAATGTTAGTGGATTTTTTCAATTTTAAATAACAAAGTTGATGGATAATATTTTAAATAG AAAAATTATATTTAAATAACTTGATTTTTATATAGTTAATACGATATAGTATTTATTGTACCGTCTATTA TACATACAATAATATATGAATTCATATATACCTTAATATAAATATTTTTAAAATATCGCGCACCTAATAC TATATTTATTTATTTCCTTCTATGGCCATTAAATGATGACATTATATTATGCACGACTATAATTATTGGA ATTTGATTCTTAATTCATGAAATTATGACTTTGAGCATTGTTTTTAAATTTTTTGAGAACCAAGTATTTG AAAATCATAATTTTTTTTATTAATTTAATTATTTACAAACTATGTAACAGGGAAATAAAATTTAATTCTT CAAAATAATAACAATTTTTTCAAATGAGAGTAAAGACTTTTCTTATTTGAAGTATTATCTAAACATACAT TATTTCGTTTTTCTTTTCCCATTTAACAAACAATTATTTACAAAAAATTATAATTTGAAGATTGGTAAGA AGATTCCGTCAACAAAATTTAATTTGCAACAACTTTTGTTATTGATATGAAATGAATATTTTCCTATTTA AACTATGTGCAAATGTATATCTAGATAATTTTAAATTTCCATTAATATCAATATTTGACAGAAAAATTAT
Page | 28
GEN 3040
AATAAAATTTGTGTTGATTTTTTTTAGGTTTATACATAAAATAATTTATTTGTTTTAGTAAATTTAAAGA ATATATTTGTGTAATAACTTTAGTAAGTCGTGATAAACATATGTATGAAAGATATATCTTGCATTCGTTT TAATATAAATATTGAATATTACACAATTGAAATATGCAAACTAGTTTAATTAGTACGAATAGTATAGGTT TCATTTTGTGAGATTTCATTCAATATAGTGTTGTTAGGAAATTGATTGTTGAAAAATGATAAATACATAA AATATAATTATACGAGAAAGTTCAAACAAGTAGCACTAGTGGTCTAGTACTAGAATAGTACCCTGCCATG GTACAGACCTGGGTTTGATTCCCGACTGGTGCATTTCAGAGCAATGGCATTTACTTGCACAAATTGTGAA CGATTGGGTCGTCATGGTACATCTGATTTATAATATGATATAATGTGTATCTGAGCTCTGTAACTTTTAC TCTTTTTTTTCACGACAATAATAATAACAATTACACCCAAAAGGATAGTGAGGTGAGATTGTCTTATCAC AAAACTAAGGATCAAAAAAAAAATTATATTGTACAAATTTGAAAATAGTAATGTATATTTGAGTTATTTG ATTGTCCATTTTTTAGTTTTTGTCACAACCTTTTAGTTTATGTTGTTTTAATTTTTTAAAAAATGTTAGA CAAGTATATGTTGAATCCTCTAAAAATAATATATTTTTAAAGAATAAACATGAATACCGAAATAGTTTTT GTTGTTAAATTTAACTATTCAACCAATTAAACTTTCAAGTACATCATAATAATCTTTATGCATAAAATAA CTTATTAAGATGCACATGAGAGCGAAGATTATCAACTATAGCCCATACATGTCTTCTCAAAGATGAGTAA AAAGGAGAAATGCAACATCAGAGTATATATACAATAGTACTTAAAGAAGTTTCAAATTGAATTTTGGTGA CGAGAGACGTCTTTGTGAGCTGTTAAACTTCATTGTAGGAAGAATTTTGCGTGAATAATTCAATCTTCAT TGAGATAATTGAATATCTATTAAGATTTTTTTTATCATCTTTATAATTTACGATTCTCACACTTATGCAA TTTAAATAACGATTCTTCCCCTCCGTAGTCGATATTAATGGTGCTTTTCTCTCTGATTAATTTGTGCACT TAATAATTTTTTTGGCAAGAAGCAATGGTTATTCTTGATATGGTTTAATTTTAAATAGGGAAATTGATTA ATGTGAATCTTTAATGTTTTAATTTTGGGATCATCGTATTTATAGAAAGTTGGTGATTTTTTTTGTTTTA GTTAACAAAGTTGGTGGATGATACTTTAAACGTGAATTTCACATTTAATTAGCTTGATTTTCACATTATT AATATTATATAATATTCATTGTACATTCAATAATATATAAATTGACGCATAATTCAATAGACTATTAAAA AATAGCATGTATAAAATATATATTTCATTAACTATAGTGATTGGAATTGTGAGTCTTATATAGTTCATAA AATTGCGTTTTCGAATTTTTCGAAAACCAACATGTATTTTGAAAATAATAAAAAAGTAAATTAATTTTAT TTTTTTTACAAGCTACTATAAACTAAGAAGGAAATAAGCTTAATCATGTGAATAATAACACTATTTTTCA AATAGGAGTAAAGACATTTCTCATTTATATTATCTAAACATAATTTTTTTCATCTTTCTTTTCTTATTTA ACAAACACGTCCAACAAAATTAAACAAAACTATTTATGAAAAATCATAATTTAAAGGTTGCTAGTGATTT TTGGAAATAAAATTATCTAATCATTTTTTTTCTTCAAAAAATCTCAAACTTAAAATTATAAGAAACAAAA AAATTAAAAAGAATAATTCGGGATAAATAAATGATTACTTTTTCAATTTCATATATTTGTTGTCGATGAA ATGAAATGAAATGGAAGTTTTCCTATACCAAAAATAATTTTAAATTTCCATTAATATCAATATTTGATTA AGCATGAAAATAAAAATTAAAATTTGTATTGAAGATTTTTTTTTTTGATTTTATATAAAAATAATTTTTT AAAAGTAATTTAAAGAATATATTTTTGCCACGTGTTTTTCCACTGGTAATTGCCTAACATAACCTTTCCA TATTATAGTCTTATCACGGGAGCTTATTGACCCTCACACTAGACAAAATCCGTCAGTTCAAGTGTGCCGT TTCACCTGAAATCGTCAGATTCCGGCGAGATGGCCGTCGAAGATTCGCCGGATTGGCCTCCTCCAGGATG GACAGAGGATGTCAAAGTATCAAAAGGAAGAAAAATCAAGGTAGAAATTGGACGTATTGAGCTAAATTTT ACTCATCATTTCTTCTACATTGATGAATCTTTATAGTACGTAATTAGTCGATTGTATGGTCAAATACGAA CAGATATATGTAAAAATTAGGATATTGTTGCTGTTATTGTTTTTTTTTGGGGATATTGTGTATAAATCCA GATGTGTTTGCGGTAATTTTTCACGGGGTCATCGGAAATTCGGATATTGTTGTTATTGTTTTTTACTATT CGTTTTTGAGCAACGGCTGATATGATGGGACAGCTCTGCAGCTCAATTTCGTTGTCATTTCTTTGAATTC GATATAAAGTTTAGTCATTTTTGGAGGTAAGTTAAAGGAATTTCGATTTGAAGTTGATAATAAGACGATT CTGTAGTTAAATCCAGATGGATTTATGGTGCTTTTTGATGGGGTTGTTCAAATTTGGGATATCAGCTTCG TAATATTTTTTAGTGCGGGATCACTTTGACAAACTACTTGCATGTAGAGTCTTGATATTGAATGGTTTAT ATGTTGTGTAACTTGAGTATTGTTCTGCTCTGTATTGCAAATTCAGAGTTTTAATTCAATTTTCCGCTTT CTAGCTTTTAGTGTTGAAGCTTTAGGTGGGCGAAGTGGGATTCAACTTCTTTTGTTCTTTCGTAAAACTA AGTTTATGAGGTGAGTACAGCAATGGTGTCTCAGGACCTGTGTGGACATAGCTTAAACATGGTAGAAAAG GATTCATATATGCATATCAACTAGTCATTATAAGGCCAGAATCATTTTAGTTTGGTTAGGAAGTTATATG TCAGAGTAGAGGTGTAGCACGGTAGCAGTAAGCGCAAGGGTTAGAGAACCTGCAGTTGTACAAAACCCCT ACTTTGCCTTTAAAAGTTTCCTCAGTCCCTGAATTCTTTCTCGATGAGTTTCATAAAGAACTTCTCTTTG GAATACTTCATGTAATTGTGTTCCCGTCTTGTCTTGGCTAATGTTGATGTATGCAGTGATGTGTAACATT AGAATACAATAATATTCCCAGTGAAATCTCACTTGTGGGGAGCCGTAACATTAGAATCTGCACTATAAAA GGTTGTATCACATATTGTTTTCATTTATCTTTTCAGTACTATACAAATGGGGAGACTGGGAAAAAGTTTT ATTCCAAGAAGGAAGTAGCTCGGTACTTGAAGACGAAAGATACTTGCGATGATGTAACTCAAGCAATGAA TATTCAAGGTAAGAGCTTCTCTGAGAACAATGTTAGTGATATGGATAATCAAGATGGGAGCTTTGCCAAG GACAATGGTAGTCAAATGGATAATCAAGATAATTGTTGCTCTGAGAACAATGTTAGTCAGGTGAGTATTG AAGGTGACTAAAGCAACTCTTACGTTCTACTGTTTTGCTATGTGATTCTATCTTCTAAGGTCTGTCTCGT GTTATTGTCAGACTGTTGGGAAACCAAATAATTCTCATGAATGGTTACCTCCAGGGTGGATAGTTGAATT GAAGACTCGTAAGTCTGGTTCACATGCTGGTTTATCTTACAAGGTATGAAAATGGAAATATACCGACCTC AGAAATGCTTGTGGTGATGATTTGTTATAATATGCGTCTCTATTTTGACATTAGTGTGACCTTTGGTAAG
Page | 29
GEN 3040
ATCAGCATTGTGTCAATTTTGGTGTTGTATCAGCACGTCTTTTTCCATGATTCCATGCTTAAAGTAAACT GATAACCGTGCTCACTCAAATTCCATGTAGATGACAAAGACTGTTGCATACAAATGATCATATGCTGGTT TTGGTCCTTCTGTTAGTTTGTCTCAAACACAAAATTCCAATAGTATTTGTTAGTTGAGGTAATGATGAGC ATGCAGATTCTAGGGGTTGAGTGGTGCATAATATAGAGAAAAGGTCCAAATTTACCCCTTTACATACAAA TAGAGGGGTAAATTTGGCATTTACTCATTTTTTTTACAAATATTTTCCTTTTATAAAAAAAAGAAGAGAA GAAGTCCAAATTTACCCCTCTACCATAAAAAAATATCTTAATTGTACTCTCGGTTATACTTTGGGGCAAT TCATACCTGATACCTTTACCACTAATAACATGTCTTGTTTTTGCCCTTGATTTTAAGGACACTCCAGCAT GGGCTGGGTGGACTCCACATGCCAATATACTTTTGGAAAATGTTCAAATTCACTCTCTACTTTTGACTAG GGTTAAAATTACCCTTCCTTATACATCAGACTGGAGTTCCTCTGTTAGGGTCAAGGGGAAAAAAAAAGAA ACGAGATTTTCATCCTAAAAAAACACACATGAGGATTTGCTAACAACGGGGTGAATGGTCATTAGCCCTC ACACATAAAGAAGGGTAAGCTACACTGTCAGCTTCATAAAAGTCAGTCCCATGTTTCTATAATTAGAAAT CACTTAACTTCAAAAATTTTCTTCTTAACCTTAATGAAATGATTTGTAACTTAACTTTAAATTTTTCCAT TTACCCTTAATGCGATAATTTATAGACATACAAATGATTCTTTTTTCACGAAAAAAGTGTCTAAGGTTTG TTTTGTACCACAAATTTCAAAAGTCTACATATCTTTCTTTAACTTTGTGCCAAGTCAAAGATGTGTCACA TAAATTGGAACAAAGGGAGTAGTATTTTAGAACTTTGTTAATAGCTGTATAATGTCTTTTAGCTTAATCA AATTTGGTCCTGTATGTGCATCAAAACCTCAGACCAGTTTAGTCATCCAGCCAACTCCTGATTCAGTAAT CTGCTGCATTTTTACAGGGTTTGATAATAAGGAAATACTATAGAGAAAACTCAAATTGAAGAAGCTTCAT GACTTAACTTCAATTATACAAATTTTGACTTCTTTTGTCATAGGAACTTCAACCAAGTATTCCTAAAAAA CTTCTTTAGGGGCTTCTGGTACCTTGGCTGTAGTCCCCCTTTTCTGTGCAACAGTTTTTTTGCTGCAACA CCTAAGCAGCAGTTGTTCTAGTTCTCGACTTCTCTGGAAGAAGCATAGCTTTAGAGCAGGAGTGATGCTA CTTTGCTGATAAAATGATCCAATCATACCTCATCTTTAGTTTACTAGTTTACATCCTTGTTTCTTTTGTT GTTCTTTCAGGTTTACATTGATCCATCAACTGGAAGCAAATTTTATTCAAAGCCTGAAGTATCTAAATAT CTCAAAACTATGAAGCAGAACAACATCGCGGGGGAAGGACAAACAGTAGGGTCTCTCCCTTTTAACTTTC TCACCATGATAAAATCAGTTTATTATTTTCCTTAATTTTTTGGATCATATGGAGCCACATATTAAAGGTG GTGCAGTCAAAACCAAAATAAAGAAACGGAAGTACTTTGGATGAGATTTGATCTCTTTATTGTTATCTTG ATCTGCGTAATTCTAATAATCTGTTTCTAAGGATATATTTAAGAGAAAAGGGAAGTTGCTTGTATTTATT TTGGCAGTGGCCTGTTGGAAATGACAAAAGTACTAAAAAGAGTTGCACCATGCATTTGTAACTAGTTTGA AATAAAAATCTAGCTTTGGGCAGACTGACTCATTTTTTGGACATTCCTAATAGGAAAGAGTACTTGAGTA GGTAAAAGTTTGATGGAGGAGGCAAGTTCCAGAAAAGAACTTGATGGTAGCTTTTGTAGCCTTAGGTTAT TACATATTTTCCACATTTCATGTAGAAGAGAATTTAAAATGCCTTACTTAAGCCGAGGGTCGATCAGAAA AAATCTCTTTACCCGCACAAGGTAGGGGTGAAGTCTGCGTACACACCACCCTCACCAGATCCGACTTTTG AGCCCAGACCTCTTACCTACCCTGGGTATTTTTGTTGTTGTTGGGTTACTACAGCATCTTCATGTGTAAT TATGTTTTTGTGTCAGCATGGAGATGGTGAAATATCTACTACAAATCAGAAAAAATCAGAGGATCCCAAA ATTATCAGGGGTGGGGGTCAGACTCGCAAATCCAAAAGGCTGAAATCTGACACCGACCAGGCCTCGGTAA AGACTATTTTTTTCTGTAATGTACTTATGATACTGCTTACAGATTTCCTTCTCAAAAAAAGACACTGCTC ACTGATTTGATTGTTAGCTATGTAGTTCACCATGGGTTACCGATTTCTCTAGTAACAAATAGGTGGCAGT TGAAAGCATTTCACTGGGTGTTGCATTTAAGAACTGCAACTTATTCCCGTCTTATTATGTTTTTTCTCAG CCTGGCATTGAGGAGGGGTCATTTTCCAAGCTGAAAGCATCTCAGGATTTTGAAGAAGTTGGGGAACAGA GTTGCACAACCAAAAGGAAGAAGTCTGGCATTACCAGGACTACAAAGACTTATCCTTCTGTAATATACTT AAGATACTTCTGAAAAAAATTGATTCTTTAATTCACTAGTAATATCTAAGTTGCTGTTGAAAGCAATTCA ACTGGTGTAGTCTTGAGATGTTGCCAGTATTCTCATGTAATAATGTTCTTGTTTCAGCATGGTGCTGGAG AGGTCTTCATTTTAAATGAGAAAACATCTCTGGGTTCGAAAGAAAATGGGGCACAAAGTCCCCAATCCAA AAGACAGAAACCTGGCAGTGGCTGTCCGTCTGCAAAGAGTGTTTCTTCTGTAATACCTCAGATGCTTCTG ATAGATATGATCTATAGCTGTTCTATTCACTACGGATTGCTAATTTTTGTAGTAATATTTAGGTTGCAGT TGACTGTGATTCAGCAGATGAATTACCCCCTGGCTGGAAAAAAGAAAGTAGGAAAAATGATCGTGGAACA AGGAATTACCTGGTATTTCTCTTTTTTCCTCAGCCCCTCCCCCACCCCTCTTACTATCTATCATCTATGT ATGTGTGTGTATATACAGGAATTTGCGAACCTACTACCTGAATGTAGTACTTAGCACCATAACCATTTTT CATTTTCCCAATATGAAAAGGGAATTGATCTATGGTTCGAGTAATTTTAATTTCGTTCATCAATAGAAAA GGGGAAGTTAGAAAGGATTTTTTGAATCCATACGTATTTAGAATATGTGCATCTTTTCATAACAAAGATA AACTTTTTGGAAATATTGTCTACCAAGGTCTTTATTGTTCATCTTCAACTCCAACAGACTTTTCCCAATT GAAAGAAACCCATCTCATTTACTCATTTCGTCTCCCCAAAGACCACCTCATGCTAAAATGGGTTCGTTAT CCCTTCCATGGTCAATCTTGGATGTGCTAATTGAAAGGCTAGAAACTAATCGAAGCCGTAACAGGAATTC CATTGAAGTAGCAGGGAGCTGGGAAAAGATTGTGGGGAGTGGGGTTGGGGAGAAGAGACTTCAACTTTTT TGGTTAAAATAAACGTCCCTTTTTTAATGAGATAGTACAGACAATTTGGGTAAATTAAAGTGGATTGTAA ACCTTTGCACAACCCATATTGTACATACAAGTATAATCTGTTTAAGGGGGACATTACGTTCTGTTTTGCA CCTTTATGCAGTATTTTAGCATGCATGAGAAAGGGTGCTGTGGAAACAGGGTTAGGGGACTCCTTGTGGG ACTGATGTGGAATGGTGTGACAGTAGATATATATTCAAAGTTGAGAATTTTATGTCGGCTTGGTCATTGC
Page | 30
GEN 3040
AGCTCTACACAGATCCAGTACATGGATATAAATTTCACTCCAAAAAGGAAGTTCTCCGATATCTACAAAC TGGAGATGCTACTAGCTGTGCTAGAAGACCTACGAAAAGGAATGTAGCTTCAACTACAAAGGATGATTCT GTAAGTCACATCTTTCTGGATCCCCCCTCCCTGTATACCCATCACTGCTGTTATTTGTACTTGTTTTATA GCCCTTTTTCGTTTAAGTGTTCAGCTTGCATACAGAACCTTGTTGGTCATCTTTGTAATGTTGCTTCGCC CTTCAGTGAGATGCACCAGTCTCAACCCTTTAGTAGGAAAACACATTCCATCCTGTAAGAGTAGTGTATT TAGTGCCTTCTAAAGCAGGCAGGCTCGTTGAAGTTGTGAAATTTTAGTCGCACTTGAAAAATCCAAGTTT GAAGAGAATAATTTCACATCTTATTATGCAAATTGAAGGATGTGTAGACAACGAGGGCATTCGGCTAATC TTATAAAATAGTTAAACTGGCTTGTAAGCACCTTTTGGCTTATTTATGCATTAGATAGCAGTCAAAGTGT TTATAAGCCTTCCAAAATTAGCCAAAAACTGTAAGTTGCTCATTCCCAACTTATGGCTTTTCAATTTGTA AGTACTTTTGGTTTGACTAAAGTTTTATGATTTTATCCTTGATATATTCTCTGATTCACCAAGTACCTTG ATAAGATGGTCTTTTGTTGTTCGTCCTTTCCCCTACAAAATATATATTTTAATATATGAAATATATTTGA GGTAGAGGGTCTTCAGAAAACAGCCTCTCTACCTCCTCGAGGTAGGGGTAGTGTCTGCTTATACTCTACC CTCCCCAGACCCTTCTTATGAGACTACAATGGGTTTGTTGTTGTTGATCTTTTGTAAAAAGGTTGTGGAT ATTTTAGTCATTTTAAAAAAAGACCATCTTATCAACACTTTTTTACCAAACACATTAGCTGTTTATTATC TTTCAGCACTTCTATCCAAATACGTTTGTTTTAAAAAAAATTCCTTCAGCACTTAAAAATACTTTGCAGC ACTTGAAGCTTATCAACTATCTACAATCTGCTAATCCAAACAAACTCAATAACTTGCAAGAAATTTAGCT AGTGACTTGTTTGGTGGATGGCAAAGGAATGGGAGAATACCTTATGATTCTAGATCTGAACCATCATCCA AATGCTATTTATTATCTTTTTCCTTGTAAGTCCATTGAATGAGGAGGAATCTGTCTGGTATTGCATATCA AATTTAATCATTCAAGTGTGAAATCGAAGTCGAATGGAAGAAGGGCATCCAAATGTGCCCAAGGTGTTTT TTTTTTTTGTTTTTGGTGAGCAAAAGGCAGAGATAATCAACCATCTTTTTTTACATTGTAAAGTGGTTAG CCAGCTATGGAACCTGTTTATTAGTTTCGGAGGCATAAGATGGACTATACCAGGAAGGGCAGGTTAGGCT CTAGTAAGCTAGAACAATGAAGGGAGTGGTAGCACAGACAAAAACATATGGAAAGTTGTCCCAGCAGTGA TATGGTAGACCATTTAGAACGAGAGCAATTCAAGATGTTTTGGAAGTATCAGTAGTCCTTAAGATTAAAA TGAACTGCAATATCACTTTTTGCTATTGGTGTTTCTGAGTATATAGATGATCCTCTAGCAATTGTAGACA TTCTATGATCCTTGTAAGATGAAATAGGACCTGGGATAGGGCTTTCTTTAAGTTGCTGAGCCTATTCAGT TGTATGTAGCTCTGTGATGTAAATTTTTGGGAACCCAACCTTAGTGCTGAAGATTTATTTACATAATTGT TACCATTGTCAAAAAAATAAAATAATCAAAGTTGAAAGACTTTTCACCGTATTAACATTTTGCAGATATG GGTATATTCTTTCTGTCTAACTGGTCGATCTTGAAATTCTTCCTCCTTGTTCTTGACTAGCCTTATTTTA GTTTCATGAAAAACTGTAATTGGTCTTGTTCCTTAGTAGACATAGTTTTTGAAAAAATTGTTGTTTATGA GCTTAGTTTGCTTATTATCAAGACTAGTGGATGTTGATTTACCCTAATGGGAGGGATCTCTCATGTTTAT AAATTTATTTCTCTTGATAGCCCACAACAGATGATGCCAGCTTGAAGAGAGTAGGAGGCTCTATGACAGG AAGACAGCTTTTTTCCACTGACGAATTGAAAGGTGATAATCTGTAGTTGGAAATATTGCTCTGTAGTCCC TATCTGAGAGGATGTGCATCGTTTATTCAGCGTCTGGTCATTTGTTCTTGTCATTTCAGTTCCCTTCATT TTATTTTTCTTTGTCAATATGTTCACCTGCTCTCTCTCTAGATGTTGGTGATTGTTTCTCAATTGTGAAA CTGGGATTACTGATGTAGGTGGGGAAAGTTTTGGTACTTGCTCACCTGCACAGCAAGTTGAGAGTTCGAA GAAACAGCCCGACTGCAGTGTGTCTGATCCTAATGCCATCATCACATCCATTTTAGCTGAGATAAATGAG AATCATTCCTTTGAAAATGTAGTTGGAGATGCTGAAATCGAAACTGTAAGTGTTGGTGTTAAACAGCCAT CAGCTGTTTCCACTGAGTCGGATCTCCTCTCGGAGAAGCAACTACTAGAAAATGAGCAGGAAAAACATAG TTCTAAAGCAACACAGCAGTCAAAAAAATCCAGAAAAACAGAGTCACTTAACCTTGGTCGTCGGGTCTCC AAAAGACTTGCAGGTCAAAATGCAGAAGCGGCGGCTGATTTGGATCTTGGTGAACGTGCTCTTCCAGCTG TAGCTGATAAATCTGCGAGCCTCTCCGTGAATGCCTGCAGCCAGGAATTGCTCCAGGACTCCGATCCTAC ACCAGAAACTGGCAACTCTGATCAGGCTTCTTTGAATGGAGACCCTTCATTGGATGATTTACCCCCAGGC TGGAAAAAAGAAATCAAAATTACTAAAAAAGCTAATGGGATAAGAAAAGACCCGGTACACTCTCTCTCTT CTTGCTTGCACATCATCCTATTCCAGCAGACTATTTCGCATCATCATGCCGTGGCAGGTTATACTTGAGA AAGGCTGTGTTATGTTAGCAGGAGTAGGAGCTGCTTATGTGAAGAATGCTGCATAAGTAGATTTACATTC AAAAGATTTTAGTGCGGATGTTAAATTGATTCTTTTATTGCAGTTATACATTGATCCAGTGGATGGCTAT GTATTTCGCTCCAAGAAGGATGTTTTCCGCTATTTACAAACTGGAGACATCAGTAGTTGTGCTATAAGAC CTGTCAAAAGGGATCTAGATGCTGCAATGACGGATAGTTCTGTAAGTCATCATATTCCTGGTTCCACCCA CCACAAAACACACACACACACCCCTCTGTTAGTTCTTTTTGTTGGATAAGTTCTTTTATCTACGTTACTG CACCTTCTGTTTTCAGTTTGATCAGTTAACGTTATCAATGATCTTTTCTTGTAAGTTCTTTTAAATGAGA AATATAATCATTACAAATTCTAGTTATAATGATATAGAAGGTTAAAAAGGAACAGCTAGATACCTCATGG TTCCCAATTCTGAACCACCATCAATGGAAATTGACATTGTCATTTCTCTTGTAAGTTTTTTAATGGGGAA ATCTTTATATCAAGTCTTTTGCATATCAAATATAACTATTCCAAGTGTAGAATTTAAGTTGATGTTGTCA TTAGAAAAGAATTTATGTTGAATGTCTTTTCATTGTATTAACATTTCAGTACATGTCTTAACACCAAATG ATGTTTGAAGCAAAAAAAAAAAAACACTATATGATAATGGAATAATCTTTTTTTTTATGAAGTAGTTGCA TTAATGGCATCAAGCAAATGCAACAAATATATTGATTACAAAAGAGAGTTTGTTGAATAAGTTACATGTA GTCCTATTTTAGGATAAGGAGCTAACTTAAGTCATAAAAATGGTCAGAGGTGTAGATAGGGGTATCTATA
Page | 31
GEN 3040
GGAGATAGATTCACCAATTAAATAAACATGAGACTTCTACCCATAAGGGAGTGATTTGGAGTTGTTATTC CATCAACACATCTGTGGCTCCTTTCTGTACAAACACACCAAGAAATGTTTGCATGGATCATTTTCCAGAT TTTCCTGATTGATTTACCAACTTTCCCCCCTGCTGTACTCCAGCTTGGCAGTTTCCACTGCCTGTCCAAG GTTGAGCCCTGGGATTTGATGTTGGACTTAAAAAGCCGAGATCTCCATCATTCATAGGCATACTTAATGT TCTCCGGCATGGCCCTGAAATTTCCACAATTGTTTTGACTAGTCCTTTAATTGGGACTGTAATCACAAAC TATGTGTTTCATCGTTTGTCATGAGTTTAAACTTTGACAAGCTAATTCTTTGAACTCAGGGCTAATGTAT GTTGATTTACTTTGAACAAGAGAATTCTCTAACGGTTGAAAATTCTCTCTTTTGGTAGCCCACAACAGTT GACACCAATTCGAAGAAATTAGTGTGCTCTGTAACCGAAAGGCAACCTTTTGCTGCCAAGGAATCCAGAG GTGATACTTCTTTAGTTAATATTCCCTAACATTCTAACTATTAGAGGTGATATATACTTAACTAAATGTT GGGGCCATCAACAGCAGTCGTTTCAATTTTTCATTCTAATCTTCTCCTTGATTTTGTCTTATCGACTTTT TAAATATACATTTTTTTCTCAATTCTGAAACTGGGATTGCTGATGTAGGTCGAAAACGTTCAGTTACTTG TTCACCAAAAGTACAAGCTGAGAGTTCCAAGAAACAGCCTGAAAGCAGTGCATCCAATGCAAACACTATT ATTACATCATCTGAAGCTGATATTATTGTGAAGCAAAATGCTGAAATAAGTCTAGACACAGAGCCAGAAA TAAGAGACTCAAGTGCAGACACAAAAGTCATAGATGCTGAAAGTAACGCTATCAAAAGGTTATCAGCTGT CTCAACCCCTCAGTCAGATCTACCCTCAGAGCAGCAATTACCAGAAAATGAGCCCGAAAAGCATATTAGT AAAGAAAAACCGGAGCAGTCAAGAAGATCCAGAGCTAAGAAACCACTCACACCTGGTCGGCGGATCTCAA AAAGACTTGTGGGCCACAGCCCAGAACCGGTGGCTGATTTGGATCTAGGTGAACGTGCTTTTCGAGCTGT TGTTAAGAAATCTGCCTCTTTGGGCATCCCCCTGAATGTCTCTGGTCAGGAATTTTCCCAGAACAAAGAT CCTACAGTAGGAACTGGCAATTCTGATCAGGCTCCTCTGAGCAGAGAAGCTTCAGGCCTTGAATTTCCAC CAGACTTGGGAAAGGAAATGCTTACCCAGAATGAAGATCTGACAGAAGGAACTGGCAATTCTGATCAGGC TTCTCTGAAGATAGAAGCTTCAGGGGATGGATTATCGCCAGGCTTGGAAAAGGAAATAATTTCCCAGAAC AAAGATCCGACCGTAGGAACTGGCAATTCTGATGAGGCTTCTCTGAGCAAAGAAGCTTCAGGAGATGGAT TATCACCAGGCTTGGAAAAGGAAATACTTTCCCAGAACAAAGACCCGACAGTAGGAACTGGCAATGCTGA TCAGGCTTCTCTGAGCAGAAAAGCTTCAGGGGATGAATTATCACCAGGCTTGAAAAAGGAAATACTTACC CATTATAAAGACCCGACAGTAGGATCTGGCAATTCTGATCAGGCTTCTTTGAGAGCTTCAGGGGATGAAT TATCACCAGGCTTGAAAAAGGAAATACTTACCCATTATAAAGACCCGACAGTAGGATCTGGCAATTCTGA TCAGGCTTATCTGAGCAGAAAAGCTTCACTAGATGAAATACCACCAGGCTGGGAAAAGGAAATGCTTGGA CAGAACAAATATTCTACTGTAGGAACTGGCAATTCTGATCAGGCTTCTCTGAGCAGAAAACTTTCACCAG ATGAAATACCCCCAGGTTGGGAGAATCAAATACCCCCAGGTTGGAAGTATGAAATACCTGCCCAGAACAA AGATCCTATAATAGGGACTAGCAATTCTGATCAAGCTTCTCTGAGCAGAGAAAAAGCCTCGCTGGATGAA ATACCCCCAGGTTGGGAAAAGGAAATACTTGCCTACAACAAAGATCCTGCAATAAGAACTGGCAATTCTG ATCAGGCTTCTCTCATCAGAGAATTAGCTTCAGCAGATGAATTCCCACCCGGATGGGAAAAGGAAATGCT TACCAGGAACAAACATCCCACAGTAGGAACTGGCAATTCTAATTCACTTGATGATATACCTCCAGGCTGG GAAAAGGAACTACTTGTCCAGCAAAAAGATCATACAGTAGGAACTGGCAATTCTTATCAGTCTTTTCTGA GCAAAGAAGCTTCAGTGGATGGATTACCACCAGGCTGGAAAACAGAATTCAGAATAAGAAAAAATGCTAG TGTGATAAAAAAGGACCCGGTATTCTCTTTCTTCCTCAGCATTTATGTCTTCCCTTTCCTCCCGGTGCAC TTCTCTCCCCCTTCTTTTCTCATGCACATCATCCTACTCTAGTAGAGTCTATTTACCACATACTAATTCA TAAAAAAAAGAGTCTGGTTTACACCATTAGGGCATCTCCTACCCATAGCAAATAGTGTTGATACTATTTT AGTGTAAACCATCTCCAACCCACTACACTAAATTTTATACTAAAAAAGAATATACTCCTTATAATCTTCT CTCTCTTCAATTTTTTATTATAATTCATATTTTCAATTTTTTTAGTACAAATAATAATAAAATAATCAAA AAGAGATTTGGTGTTGATGAATAGTGTTGCACCAAATTTTGTGCAGCACTATTATCACACTAAAATAGTG TAAGAATTGGTGTCGGGTTGGAGCACTAAAACACAAAATTTTACATCAAAGTAGGATTTGGTGTAAAATT TGGTGTTTGGTTGGAGACGATCTTATGCAGATATGTGATATATGCATGAGAAAGGGTGCTTCTGGAGAAG GGTCATATAGCTGCTTATGTGGCAGACATGGAATGCTGCATTCTTATTTTGCAGTGAGAATTTTAAGTTT ACTTGGACATTGCAGTATTACACGGATCCAGTGCATGGATATGTATTTCGTTCCAAAAAGGATGTTATGC GCTATCTGCAAACTGGAGACATCAGAAGTTGTGCTGTGAAGCCTACCAAAAGGGATCCGGGTTCGACAAT GAAGGATAATTCTGTAAGTCTGCACCTTCTGGATCTCCCACCCGTGTTCCCCATCAAATCTATTTGTGCT ATATGTTCGATAACTCCTGTTATCCATGTTACTGCTTTTCAGCTTGCGTAATAATCTTGTTGGTCGTATT TGTATGAGCCTTTTACTAGGAAGATATATTTGTTTCTTTAAGACCAGTGGTAAAGTTGGTGTCTTGTGTA AAACTTTGTTTTACGTATTGGAGGTGTTGAAGCACCTCTTCTTACACGGGTTTGAGTGGTCTGAGACTGA GCAACTGATTTAGAGAATACTCGTTGAGAAGATATATTGGAAAGCAATAACTCCAAGTTGAAATAGAATA ATTCCACATCACTCTATTCTAATTACATATGTAAAAAGAAAAGCTTATACGAAACTACAGGTAAAGACTA TGCCATAGAAGGGTAAGAGGAATGACTCTTACCTTATACTTCCCAAATCTGGACCACAGTTCTGAGCTTT ATCCCTGTTTCTATAAGTTCATTAAATGTGGAATTTCTTTATAAACTATAGCTGCAAAACGATGCGCTGG AAAGTTAGAAGAATGACTCAATACCTTAATGATTTCGAGATTTCAACCACCATTTAAGCATTTTACAAGT CCCTTTTTTCCTTGCGAGTTCTTAAACAAGGAGGAAAATCTTTTATGTGTGGCGTTTTGCTTTAAATTAA CATTTCGCTGCATGTCTTTACTTTCTGTTCCTATCTGACAAAATAATCTGGAAATCCTTCACTTAATTCT
Page | 32
GEN 3040
TGATTGGCCCTTATTTGGTTCAATAGAGGACTGTTGTCACAAATCCTTAATGCATTTCACTGTTCATGAC AAGATTTGAGTTCGGCAAGGTCATTTTTAATTTCTCGATACAAGTGTATTGATTTATCTTGAAAGGGAGG AAGTTCTGATGGTTGAAAGCTTCTTTCTTTTGACAGCCCTCAACACATGATACCAGTTCGAAGAAATTAA GGTGCTCTTTGACCGGAATACAACTTTCTGCCACTGATGAATCAAAAGGTGATGCATTTTAGTGGGAATT TTGCTCAAACTCCAATACTGTAGGAGATACAACATTATTCAGTATCGACAGTCATTTCAATTCCTCCAAT TTAGTCTTGTCATTGATATACGTCCTCCTGAATTCTCTAAAAGTTGATTGTTATTCTCATTCTGAAATGG GAGTTGCTGATGCAGGTAGTAAGTGTTCTGTTACTTGTTCAATGGCACTGCAAGCTGAGAATTCCAGTGA ACATCCTAAAAGCAGTATGTTCAACCTCAACACCAGTATTTCATCTATTGTAGCCGATATAACAGAAAAA CATTCATATGAAAATCTAAATGAAGATACTGAAATAAGACTAGATGTGGAACCAAAAATAAGAGACTCAG TTGGAAACACAAAAGCTGCAGCTGCTGAAACTGTTGATGTTAGAAGGTCATTAGTTGTTTCCCCCCAGTT GGACTTCCTCCCAGAGCAGCAATTACGAGAAAATGAGAAGGAAAAGCATAGTAATAAGGGAGTGCCAGCA AGGTTGAGAAAAATCAGAAACAGCAAGTCATCTACTCCAGTTCGTCGTGTCTCAAAGAGACTTTCACGCC ACAACCCAGAAATGGTGACTGATTTAAATCTAGGTGAACGTGCTCTTCATTCTGTGGTTAACAGTTCAGC CTCTTCAGGCCTTCCCATGAATGTCTCTGGTGGGCAATCGGCCCAGCAAACTGATCTAGCAACTGGGGAT TCTGATCAGCCCTCTCTAAGCAGAAAAGCTTCATCACGAGATGATCCTTTAGAAGTTGTGAAGTGTCTTT CAGAGGGCCAAGCGTTTAAAGAGAATATTGATGAGAGAATATGGCGAGATTCTCAGCCAGCTGGAATATC ATATGAAAGAGGAAAGGTTCACTTGGAACAGCATGCGCTTAACGAAAGGCCTACAGCCAAGCCGGCAAAA GAAGAAGAAGACGGTCAGAATAGATTATTGAACAATTCTCAACTAGCTGAATCAGCATCAGGAAGAAGGG TATTCCCTGCAGAGGACCGGTCTGTCTCAGAAAGGTCTACTGTAGAAGAGGTAAGTGAAAAACGAAATGG TGAGTACAAGCAATGGCAATATCCACAACTAGCTGAACCATCATGGAGAAGTGTGGATCTTAATGTGGAA AACCAGCAATGGCAAGATGTACAACTAGCTCCATCACAGAGATTCGTGGATCTTAATATGGAAAACCAAG AGTGGCAAGATTTACGTCTACCTCCATCACAGAGAAGTGTGGATCTTAATGTGGGAAACCAGCAGTGGCA AGCCTCACAATTAGCTGAACCATCACACAGAAATCTAGATCTTAATGTGGATAGCCAGCCTGTTAAAGAA AAGCAAACAGGAGAGCTGGTAACTGAGAATCAAGATGAACAGAACAGAAGATTGCATGCGCAACTAGCTT CTTATCCATTTGGGGACTATTGGTCAGACCCATGCATGGAGTTTGCATTCAAGACCCTTACAGGTGCACT ACCAGTAGAAGATACCCTGACCTTTCAGGGATCTACCCATCAAGAATACAATACTTCCTACACGCAGGCC GATGATGGATGTTTTGAACTGCCATTATTCAACACTTCCAGTTTTTACTTGAATGATGCTCCAAACCACT GTGCTCCATCGGTGGAGCACGTCGTCAAAGAGCAACCGCCAATAAATCAGACCTTTTTGCCCACTGGACA TAATAGCATACCAGGCCACAGTAGTGTTGTTTCTCAAAATCCAGGTTTGACCTTTTTGCCCAATGGCAAT AACAGCATACCAGGTTGCAGTAGTGTTGTTTCTCAAAATCCAGGTCTGAATACCCAGGCAAAGGACTACC AGTCTAAATTTAAATCTCACAGGTGAATGAGAGCACATAAAGAGAGGGAAGTGTTAGGTGTATTAATGGA TGAACTAATGACGCGGTGCTGCTCCTGTGAATGCATTTTTATGCAGCTATATCCTCTGTTTGTAAACATT TCTCTGATGCGGTTTCTGGAGTTTGGCGTATCTATAATTGATTGAAGATTGACCACAGGTGATGCTTCAT CAATTGTCTGGAGGTGACAGATAAATTAGGTTTGTTCGAACCAGATAGCTGGTCGAAATGTAGCTTTAAC TTTCAGAGTGCTTAGATCCCACGATCCATTGGACTTTAGACGATCTGTTTCAAAAGACATTATAGACAAT TGCTTGCTATATGTCTGTATAATTTAGGTTGGACCTCTAAGCTTTTATTACACGTAAATTCGTCTCTGTT TTCTCTCCACCTCTATGACTGGATGTTAAAAGGCATCCATCCAAAAGATGCTTCTTCAAACAGGTTACTT ATTTGTGAAAAGATTAGAAATGTGCACTCAAACAGAGTGCAGTAGTATGCACCTTTTTGAGACCCATGTT CATATTCCAGTAGGTACCATAAAAAACTCATCTATCCATTCTTTAGGTGAAATAGTCGTGGCGAGTTGGA TCGAACACCATCATATGTTTTGAAAATGAGAAGAAATAAAGAGCGTTAAGGACAAAGTTAGAGTGATTTC TTCACATCATATGTTTTGAAAATGAGAAGAAATAAAGAGCGTTAAGGACAAAGTTAGAGTGATTTCTTCA CAATAATTTCTAGATATTTGACTTGATTATCTCATAACTTGACATTGTTGTTCTGCATAATAACAAACCT TACATTGTAGCATATGATAAAATAATTTAACTTGAAAAATGAAAAGAAAATCCCAACATTTTTATTACAG TAACCCGTATAGACTCAAAATAGCTCTTTTCTTTTTTACTATATGAAATCCACACTTTTAACACTTGAAA ATGTTATAACGGGTAAGATAAGTTCATAATTTAAGCAAGAACAAATTGATCAGTCGAGTAATGCTTCACA TCAATAAGAAGACTCTTTCCATCCACCAAGGTTCTCTTCTTCATACTCCCTCTCAGAATATTCCTACAAA AACATAGCAAAATATATCATGCACATAGAATACCTATTATACTTGCATGTTACGAGTTCCTTTATTCATA TAGCCAATCTGAACTTGTTAGCTCGGGATTGAGGCATAACCTTTTTTTTTTTTTTTTTTATAAATCAATA AAATTTTAGAAAGTAACTTGCCTCAGGAGTAAAGGTGAGACCAACTTTAATTTCTCCACAATATTCCTCA TCTTTAACAACATTATAACATGTTATTGGGATGCTCCCTTCTGAAAAAACTGGTTCAATTGGTATCCTGC AAGTAACATATTATTGAACATATCTCAAAACCAATACTAGCAAACTCAGATTATGTTTGCATATATATAT ATATATATATATACAGAAATTGTATATACAGAAATTGTATAAATCTTAGATTCGTCTCTGATAGGTAAAT GCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAA TAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAA TGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTA ATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAA ATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCAAATGCTTATTCGACTCCATGAACT
Page | 33
GEN 3040
AATAATCTCAACTGTTTTAGGGAAATGCTTATTCGACTCCATGAACTAATAATCTCAACTGTTTTAGGCA AATGCTTATTCGACTCCATGAACTAATAATCTCAACCGTTTAAACCATGGTTTGTATAACACCACTAGAA AGTAGATGTATACTACAACAACCACAAAAATAACTATATATTCAGTGTAATTTCACAAGTTGGATATTGG GAAGGTAGGATATCGATGTACGCTGAACATACCCCTACCTTTGCAGGTTGTTCACGAAGGACCCTGAACT CAAAAGAAAAGAAAAGAAAAAGCATGGTGCAAGTAAATAAAACGACAAAATAGCAAGATACAAATCAAAT GAAGCAACTGATTGTAATAATCATTAAAGAAAGGAAACTACGCGATATACAAAATACTACTACTAAAAAG GAGAAGTAGAGACTAGGCTAGCCCTCTTCCTCTCCAATAACAAGTGCAACAACACTCAGGCTCACTAACT ACTAACCTTCTACCCTAATTTTCGACCTCCACGTCTTCTTATCTAAAGTCATATACGTCTATTTATCTCA ACATATACAAGTCATGGATGCATGTACTATCAAACTCAAACTATGTTAATATAGAGAAAATACGTATAAT CTTAGATTCGCCTCTAATTAGATATACTTACTTAGCTTCCCCAACAATATCGTCCTCGCCGATAGAATCA CTGTCCATTATCTTCAAGAAGAGTTCTTCAGCATCCTCAGAAATGGAGAAAACAAATGTTTCATTCCATT CTGGTTCACATCCCTTCCCTATGAAAATTTAACATTATAAATATCGATTGATCAAACTTCATTTCATTTT TCTTAAAAAAAACATAGGTAACTAATAACTAAGTAATAATTAACCAAAGATTATTAACAAATGTACCTGA TGCAACAGTACTCTTTTTCTCCTGAGATCTATAAGTTAAGATCACATATGGATCCATATCATCTGTATCA CACAAAATAACATTTTTTAAATTAAAAAATAAAATAAAATACAGAGTATCAATCAAATTAGCCAAGATGA TTATCGATTTTTGATTCATATTCCTCGATTCTATTTTATTTGACATCGTTTGACTAGGCATAGACCTAAA TGTCAACTTCACATACTTTGATTCAAAATTTAAAGTTTACCACGAAGTCAAATTAATATACATTGATAAG GTTTGGCCATGAATTTTGACAAATATCTGTTTGATCATGAAAATTTGATCCGATCTTCAAATAATTCTCT ATTTTCCTAAATAAAACGCACATTCAAACAGGTGTTGTGTGAACACATAAATTACATGTAGATCAACCTC TAATTCACATAAGTAACAATCGAAAATTAGGTTGATTAAGAAAACATCAAATGTAATCGAAAGAAAAAAG AGATCTATACATTAATCTGATCAGATCAAAACAAATAAATTAGAAAAAAAAACAAAAAACTTACTGAGAA AATCAGTGTTTTCAAGGCCTTTAGCTCCAACAAGTTGAACTACAAGTTTTCCTCGTACCATTTTCAATCA AACTCTTAGCAGATTCCCAGGAAAAAAAAACAGATTCTCGCACGAATTTTCGGACAGAGATCGTTTTCTT TTTTTTTTTTTTTGTTTCGATGAAGAATATGTTGAGCTGATGTGTATTTATATGTGAACAAAAGACCGGT TTAAAGTGGGGTTAGACCGGTGAGTGTAAAGTGAACCGGACGGTTTAGGCGAAAATCACATTTTAGACCA AAAAAGGCAAAAAAGAAAATCACATTTTACGCACCTAAATTATTTCGAGTATGAAAAACATATTTAAACT AATAATAATAAGTTTGAGAAATATACTTCAAGTAATATGTGATGTCGTGTGTATTACGTGTATACACACA TTTGATTATGTTAGATTGCGTTGGCGAAAAAAAAGCCACGTGGATTATTTTTTTTTTTGGATCAAATGCG TCGGAGTGTGCATTTGGTCTGGTTAGAATTGAATTGTGTTTTTTTTTCTTCAAATTAATGAGTGGTAATA TTGTAGAAATGAATCAATAGAATCTTCTTCATTTTAAGATTTTAGGTTTCAATTATTCGTTTTTGTGACT GAAAAAAGGCTAATTCAGCAGCTTTTTGTTCAATTTCTCGTGCAGTCAAATTCTCATCAGTACAGGTCAA GAGAATGGACCCCTGGCCTCGGATGTCCATATAAAGAACACGACAAACATAAATATCCTCTTTCACTTCT ATTCTAACGAACTGAATATCTTTTATAAGGAATCGGAGTACTTAGGAACATGTTTTATACATTTTCAATA ATTTAAATATGATATTAAAAAAAAAAGATTGTTCAAATATGTTTTGACATTTTTAGCTTTTATTTTAATA AAATAGATTTAAAAAAAAGTATCTTTGAACTACGTGAAATTCAACAAATGTCTTCTTCAAAAATGCCATC CTATTAATATTCTTGTCCAAACATATCCCTACCATGAATATTTTAGTCAAAAAATATCCTATTACTACTT TTGAGTAAAAAATATCTTTTTAAAGTATTGTTTCTTTTCTGTTTAAACAAATTCCCTTTTTAAGAGATAT TTGATATATACCCTTTGGGCCTTGATGGTTGATGAGGTGATCCAAGAAATATTTAGACTATCATTTAATG CTTGTTTAACAAACGAATATTAAGAAAGTGATTTAACATTTTTTCTTTATAAGATTTACCGTTTGTTTCA AAAATAAATTTCGATTTCACCGTTTAATAGATTTGGTAAAAAAAGTGATGATCGTAAATTTTTTTTTATG ATTTATTAATATCACTCCCTTTTAGTGATATATCATACATTTCTTATTTTTTTAAAATGGATATTATGTG CAGATTATTATCTCAAGTGGACATATTATGACCAAGCAAAATTATTAGGTTTTGTATATGTTCATTGTAC ATAATAATTGTATATGCTTATAGCCGCCTATCTAGCTATATCTTTATAAAAAAATATAAATTATGGTTTT ATTAATATATCAAGTGTCTATATATACATATATATATATATATATTTATAATATTGTCCATAGTGTAAAA AGCCGCCATACCCCTATATAAACCCCTAATTTATTGTGTAAATATATCAACAAACCAAATATTCTTCTTT TATTCTAAAATGAGTCATTTGACAACTTGTTTAGTGTTCTTTCTCCTTGCCTTTGTGACTTACACTTATG CTTCCGGTGTATTTGAGGTCCATAACAACTGTCCATACACCGTATGGGCAGCGGCGACTCCCGTAGGAGG TGGCCGACGTCTCGAGAGAGGTCAAAGTTGGTGGTTTTGGGCTCCACCGGGCACCAAAATGGCACGTATT TGGGGTCGTACTAATTGCAATTTTGATGGTGCTGGTAGAGGTTGGTGCCAGACCGGTGATTGTGGTGGAG TCTTGGAATGCAAAGGATGGGGTAAACCACCAAACACCTTAGCTGAATACGCTTTGAATCAATTTAGCAA CTTAGATTTCTGGGACATTTCTGTTATTGATGGATTCAACATCCCTATGTCTTTCGGCCCAACTAACCCT GGGCCGGGAAAATGTCATCCAATTCAATGTGTTGCTAATATAAACGGTGAATGCCCTGGTTCACTTAGGG TACCCGGAGGATGTAACAACCCTTGTACTACATTCGGAGGACAACAATATTGTTGCACTCAAGGTCCATG TGGTCCTACCGATTTGTCAAGATTTTTCAAACAAAGATGTCCCGATGCCTATAGTTATCCTCAAGACGAT CCAACAAGTACTTTTACTTGTCAGAGTTGGACTACAGACTACAAGGTTATGTTTTGTCCTTATGGCTCTA CTCACAATGAAACAACAAATTTCCCATTGGAGATTCCTACAAATACTCTTGAAGTGGCTTAATTAAGTAG AAATCTTGAGTTGAGCCAATCTCTTTTTTGTTAAATTTGTTTTGAAGATGGACATTGTCTTTAATTTGCC
Page | 34
GEN 3040
TTGTACACATTTCTTTTACATTTGTTTGTTTGAAGTGGCTAAGATCTGAATAACAATCTATGATCGATGC TTTGTCTTATATATGGTATTTTAATTTTAAATTGAAATTATATCTGGAGTACAGTTTTTTTCTTAAAATT TTTAATTATAATGAAAAAAAATTAGTCTAGTTAATATATTAAAGTACCTAGATCTGAAAATATAAAATTG TAGGGACAACTCGTTACATGTCATAATATAAGTCAAACAGATGGAAATATTTTTTTATTGGCGGCTTTTG GAACATTTTCAAGTATACTATAGTATATGAAAATTCATTAAAATTTTAATAAGTGTTTAGATTTGAATTT ATAAGTTTAAAGAAATAATAAATTCAATATTAATTAAAAAGCTTAAAGATTCGAACATTAAGTTAAACTG ATAATTAAATGAGTCGATATACAAAAAAGAAAAAGAAAAAAGAAGAAGATAATTTTATATAAAAGTCAAC TTTTAAAAATCACTTACCCCATTAATATCTTCCTTCTAAAAGACGTTATCCTTTAGTATCTGAATTCATA ACTTGAAATAGCAAAAAAGGGCTTTCACTTTCAATTGCGATTCAATGCCTAAGCTAAGAATTCAACATTA TTTAAATATTGATATTGTATTAAAATTCATTACTTCCAAAACTCTTTTATTATAAAAAAATAAAATAAAA TACAATTGGGCGTTCGTATTGCTTAAATAATTGCAATGTTACTTAAAAAATTCTATTAAAACAATTTAAG TTCTTTTATTCTGCCCAAGCAGTTACACGTTGTTGATTTCAGACTCCTCCGGTAGAGCCAACAACCATTC AGTGAATTGTCTTGTGTTACTAAGTTTCAAGTCGTTCACTGAATCCTTCACGATGTTTTGGTGGCAGGTA CTAGTACATCTACCGATCAAAATCAAATCCAAAAACTCTTGCATGTTCCAAAATTAAACTCAAATATACA ATCATCTCATTTAAACTCAAGAATATCTTATTATGAACCAACAAACAAAGACGACCATGATGACAACGAT AAAAAATCAATACTCCCTCCGTCCAGAAATATTTGTCATGTTACGCTTATCGAAAGTCAATTTTGATTGA TTTCTAAAGGTAAATTAAATCACATTAATTCGATACTTTAAACCAAAAAAATTAAATATTCTAAAACAAT ATGAAAAGTACTATAAATTACAATCTTTTCATATTAATATGATAAAAAAATGTATCTTCAAATGTTAGTC AAAGTTTTTATAGTTTGAGTCTATAAATAGAAACCATAACAAACAATATCGGACGGAGGAAACATAAAAC AAAAATCAATATCGAACAAATTATTCAATCAATAACAAAAATCATTGCTAGTTCTATATCTAGTAACCAA TTAGGGATAGATTATTCAAAACTTTGCATATTAGAGATAGATTAGTAACGAAATTCATAACTAATTTCAA AATTCATTTTTCTCGTGTGAAACAAGACCTACATAGCTTGTATCGAATAAAAAAAAAATTCAAACTTTTA AGGTTCCACTCATCTCTACGCCTACCCATTTGCTCAACTTTTTTGACTGTAGAATGCATGCACATTTTCA ATTAATTATTATGTCCACTTGATAAATATGCTATAAATAATTTAATATAAAAATCCACTATATATATACG ACGGAAAAAAATAATCAAGGGAAAAAAGGCAATTGTCTGTAAACAAAAATTACAAATTTGTAATTCAAAA TTTTTAAGTTATTTTATTTATGAAAACATAACCAATAAAAATTATGAAGAGAATTCTCTACTAACTAAAT TTCTCATGAAAAAATGTTCAAAAAAAATTAGGAAATTTTCCACAATTTTAGGTTTCATGCCCTGCCATGC ATTTTTCCGATTTGTTGTATATACTAAACTAAATAATTTAAAATTAATATATAGTTAAACACAAAACTAA TAATACGTATATATCACTTAATTAAAAAATATCTTCTCTTTTTTTTTTAATCATTATAAATATCATAATG TGTTAATGCATAAATTTTCAAAATAGCAAAAAGTTGAACTGTGAAATATGACTGGAGTGAACAAAATATA TTACTTATAATAGGCTGTACTCATATTATATTGGCGGCTTTTTTTTTCAAGTGGAGATATTTTGGATACA ATTTTTTTTTTTAATTTTCTATATATGTTTATAACAAGTGCCAATATTTATATGATTAATGTCCATAGTG AAAAAAAGCCGCCATACCACTATATAAACCCCTAGCTCGATATGCAAATATATCCTACAACTTCTTATAC TAAAATGAGTCACTTGACAACTTGTTTAGTGTTTTTTCTCCTTGCCTTTGTGACTTACACTTATGGTTCC GGTGTATTTGAGGTCCATAACAACTGCCCCTACACCGTTTGGGCGGCAGCGGTCCCCGTAGGAGGTGGTC GACGTCTCGAGAGAGGTCAGAGTTGGTGGTTTTGGGCCCCACCTGGCACTAAAATGGCACGTATTTGGGG TCGTACTAATTGCAACTTTGATGGTGCTGGAAGAGGTTGGTGCCAGACTGGTGATTGTGGTGGAGTCCTG GATTGCAAAGGATGGGGTAAACCACCAAACACCTTAGCTGAATACGCTTTGAACCAGTTTGGTAACCTAG ATTTTTGGGATATTTCTGTTATTGATGGATTCAACATCCCTATGTCTTTTGGCCCAACTAAGCCTGGCCC TGGAAAATGTCATCCAATTCAATGCACAGCCAATATAAACGGTGAATGCCCTGGTTCACTTAGGGTACCT GGAGGATGTAACAACCCTTGTACAACATTCGGAGGACAACAATATTGTTGTAATCACGGTCCATGTGGTC CTACTGAATTGTCAAGATTTTTCAAACAAAGATGTCCTGATGCCTATAGTTACCCTCAAGACGATCCAAC TAGTACTTTTACTTGTCAGAGTTGGACTACAGACTACAAGATAATGTTCTGTCCTTATGGCTCTACTCAC AATGAAACAACAAATTTCCCATTGGAGATGCCTACAAGTACTCTTGAAGTTGCTTAATAAGTAGATTCAT CATGAGTGTAGCCACCTCTTTCTTGTTCAAAATGGTTGAGTTTTGTTCATTACTTGTCTTTTGTATTATC CACATTTCTTTTAGTTTGTTATCTTGAGGTGGCTAAGATTTCAATAACATATTGTACTTTAATTTGTCTT ACACATGATGTTCAAAATTCTCACACAAGAGTGCATTTGGAGTATCGTTTAATTTTGTCTTTTTAATTCC TAATTATCTAAGTCTTAAACCTTAGACAAAAAAAGGAATGAAAAATAGTATTGATAACATTCTACCGTAA TCAGATCAAAAATATAAAATTGTAGGGACAAGATAAACTATTATAATTACTATATAGGATAGCTATCTTT ATAGGACATAATAATTAGTCGAGGTGGAAATCTTTTTTGTAGGCGGCTTTTAGAATATTTCATGCAAAGG TTAAGGTGGAGCCACGTAGAATTAAGGGGTTTATTCAATTTTTTAAATAGATAATTATTAATTTTATTTA TATATAGTTAAAATTATTTTTAATGTATATACAGTGATTAATTAATGAATGAATTTTTTTTTTCTTTAAA TTGTTAGTGTGTTTATTTTCTCATATTTCAAAATCCTCTAATGAAAATTATTACTCCGCTATTAATCATG CTCTTGATATTCAACATTTGTATTGAGCTTGATAAATTTAAATTTGCATTCGAAAAATTTCACTTTGAAG GCAAAACACTTATTATATATAATGAAAAGAAATTTACTCTCATGGCTCAAACTCGATATATCAAATTAAA AATTGAAAACTAACATCCCACCACAGCCCCTTTGCATATTTTCAGCACGTTATGGTGCATATTCCGTGAC TTTTATGCAATCACTCCTGATAATAAGAAGCAAAGTCCTTAGCACCATAATATTCAATTCATTTTAAAAT
Page | 35
GEN 3040
ATTTATCATGTTTTATTTGAAATAACTGATCATTAACAAAATATGTTCAAATAATTTCACTCTTTTTTTT CTTAAAACTTAACTTAATAATTTAAATTCACATTACGTAAACTTTTTAACTCCACGCCATTATTATAAAA AAATATGTCATGTGTCAAGTGGATATATAATTAAAAGAAAAACGTATGTGTATCTACACTCAAAAAAGTC AAGAAAATGGGGGTATATGGAGATATTGGTGAGTGGGGTTTTTTATGTTACTTTGGTGGTTAAATAATTA TTCATCATTACTTACTATATTGACAAAAGAATATTATTTTCAAGAGTAATGTTGTTAATTGTTTTAGGAC AACTTTCGCATATAGCAAATAAAAAATTTATATTTGTGTGTTATAGCAAAATTTGTATAATTACGCTCCA TAGCAAACATAAAACTGTATAATTCACTATAATATACAATTGTATAATTTGCTGGCCTATTTCGCCGCAA TTGTATAATTCGCTATCATATTTCACTGCAATTGTATAATGCATATTGTATAATTCTCTGCAATATTTGT ATAAAATTTATTTTGCATACAATTGAATCGAAGTAAAATGTATGTATATTGCATAATTATAAGTGTATAG GAAAAAAATATATGTTTTTCTCTCGCTTTATACAAAAACATAAACACAATTTATACACTTCTGTTGTATA AAGCAAGAGAAAATTGTATTTCACTGCAATTGTATAATTCGTTCGCCTTTTTCGCTGCAATATTTGTATA AAATTTGCATTTGTCTACAATTGAATTGAAGTAAAATATTTGTAAATTGTATAATTAAGTGTATAACACG AAGATATATGTTTTTGCATGTGTATATACAGTTTTCTCTCGCTTATACAAAACAGAAACACAATTTATAC ACTTTTGTGTATAAAGAGAGAGAGGCGACTGACAAACTTTGGCTAACGTTTGCTATGGTGCACAATTAAA TCAAATCATAGCTACTCCATTTATTTTAGGTTATTAGTTTGCTATTATATACAATTATCCCATTATTTTA TCCCAATACAATATTTAGTCATTTCATTCCATCAATTTCGGTGAAGTTCAAATCAATCGTTGTTTTAAAA GAGATTAGTATTGATAAAGTCACGTTCTACAATATTTTTTATTTTTTTGAGTGGCGATAAATTATTAATA TTATTTTTGACAATTCATTTTATTCTTTAGAGTGCGATATAATCAACTTGTTAATCTATTATATCAAGTT AACAAGTTATTAGAATCTTTTTCACTCTTAATCCTCTAAAATATGTAAAACATGTTAATGATAAAAAAAA AATAGTGAATTTTCTACGTTTATAAATATAATTTTTTTTAATAAAAATATAAATTTTGCTCAATTTAATC TTTTGGCATTGTGTCAAGTAGCACGTGCAGCTAACATCATTTAGCTTGAAAATGTTCTAAGAGCCGCCTA TAAACAAATATTTCCATCTACTTTTGACTTTGCCCTAAAATTGTGGATAATAATTTCTAATAAAATGGGA TTATTTTATTTTATATTATTTTAAGGTACTAGTTACTTTTGAGATTAATTTATTTTATTTTTATATTTTG TGATGGAACTTTTATCATATAAAAAAATGAGATAAAATAATCTCGTGAGATGTTCTTATTTATCTTATCC AGTATATTATAGTTTTTATGTTTTTAAACTGACTGATATTATTCTTTTATTTGACAAGTTAATACGTAAA TAACAAAATATACATAAAATAATATTCGAAACAACATATTTGTGATGATGTGACTTTTATTCATGTAATT ATATTAGGTTTATTACAAGCCAATTATATAACTCGACTACTTCAAGCAATTTAAAAGAAAGTGACTCAAT TTTACTTAGCCTCTTCATCACTTGAGGGCATCTCCAAGGGGAAATTTGGGCTAGTAACACCATTAGGACA AAAAACAACCCTATAATTTGTACTACCACTAGGGCAAGTAAATGTGCTAGTAGGATCATCTTGTGGGTAG CTATACGCATCAGGACATCTTTGTTTGAAAAATCTCGACAAATCAGTAGGGCCACATGGACCTTGTGTGC AACAATATTGTTGTCCTCCGAACGTGGTACAAGGATTGTTACATCCTCCGGGTACCCTAAGTGAACCAGG ACATTCACCATTTATATTAGCCGTACAATGAATTGCATGGCATTTCCCTCCACTAGGATTGGTCGGGGCG AAAGTCATTGGAATATTAAATCCATCGACTAAAGAAATGTCCCAGAAATCTAGGTTGCTAAACTGGTCCA AGGCGTACTCGGCCAGGGTGTTTGGTGGTTTGCCCCACCCGGTACATTGCAAGACCCCACCACAATCACC AGTCTGACATGAACCTCTACCAGCACCATCAAAGTTGCAATTCGTACGACCCCATATACGTGCCATCTTA GTGCCCCTCGGTGCATTGATGACCCATGTTTGGCCTCGATCAAGACGTCGACCACCGCCTATTGGGGTCG ACGCCGCCCAGACGGTGTATGGACAGTTGTTGCGTACCTCGAAAGTGGCAGCATAAGTGTAAGTCACAAA AGCAAGAAGGAAGAAAACAAAAGAAGATCTCAAGTAGGCCATGTTTGTGGAACTATTTTTTTAAATGTAA GTTGTTGGATTTAGTGACAAATTGTTAGGGTGTTTATATAGGGGTGTGGCGGCTTTTGAAACTATGTATA TTAATCACAAATATCAATATCACAATTGACTAATCAAACGAATAATATTGAAAAAATACTTAAGTAAAGG TTGAGGTGGCTCAACATATAAGAGCCGCCTAGTAATTACTCCATTACTTTATATAAAAATAAATATTTAA TTATTATTTTTTTCAATTTCTTATTTAATATTAAGGTATTTTTGTCCTGATTAAATTTAAATTTACAAAT TAATAGCATTTTTTATAAGATTTTTTTATTTTCAAGATTTACAAATTTTCATTTCTAATTAAGAATCGAG AAATTTCGGTCCGCTACAATCCATATATGGATAACATAGGGACAAATTTATTTGGTATAACGTGATAAGA CTTTTTCTTAACTTAATTAAAAGAAGTAACCATTATTTTTCTTTCATTGACAGAGTTTCTTTTGTACTCC CATTAAATTATTTTTCATTTTGTGTGATGTTTAAAATCATAAATAAAAAAAAAATAATTTTATTAATTCA CTTTTTTAAAAAAGTTAAAAATTTATAAATAAATATGAACACTTTCAAAAGAGTCAATTACAAGAATAAT ATAAGAAAAATAAATTAATATTTTTTTATTTACTAAAATAATTAACTAATATAAAATCAATGGAGTACTT ATCTTGTCATTTTTTATTTCGTGTTCAAGTTTCACGCGTATTATTTGGAGGCGAAATTTATGGATCGCAA TCAATAAAATAAGGGAAGTTAATCTAATTAGGTAGCATCTAAAATCTCAATTAAAAAAACGAAAAATTAC ATTAATGACGTATAAAAACTTCAAATAATTACATGTTTTATATTCAATTCAATTCAAGAAATATTATTCT CTTAAAATATAATACAGCCCATACCCTTTCCCATTGATTAAGGTTGATTATTTTTGCTTAATACATATTA AATCAGTATTATATATTATATATTGTATTGATATCATTAAAATTTATAATTTCATTTAATTGATACTAAA TCAAATATATGTATATATATATATATTGCCTATGTATTATCACTGGCAACCTAAGATCTATTTGTTATTA ATTTTCGTCGCAAATTTAATTATCATTAATTATGGAGTTAATTACTTGCAGTGACATTATGGTCGCCACT AAAAATATTCAGTGTCGATTTAAAGAGGCTCATATACATGACAGCGTGAAATGATTCTCGAGGCATTATC AATACTTTTTTGTTTGTTTATTGGTCTAATTTAGGCTTAATACTTTTCTAAAAGTTATTTTTTTTTTAAA
Page | 36
GEN 3040
AAAAAAGAACGATAATTCAAATGCACTGTTGTTTTATACATCTAAGACAAAAGTACACATGAGATGTAAC TCTTATTCCGGTCTTTAAGTTTGTTACGAACTCTTATCAATAAAGTAAAAGTGAATATTTAAAAAACTGA GCTATTAAGATTTTTCATTACGGTAGAGATCAGTTGACTAATTGAGGCGATTTAAAATGAAGAAACTCAA ATTCACTTGGCCACTTCATCAGTACTTGCAGGCATCTCCAAGGGGAAATTTGGATCAGCAACACCATTAG GACAAAAGACAACCCTATAGTTTGTACTACCACCAGGGCAAGTAAATGTGCTTGTAGGATCGTCTTGTGG GTAGCTATAAGCATCGGGACATCTTTTCTTGAAAAATTTGGACAACTCTGTAGGGCCACATGGACCTTGG GTGCAACAATATTGTTGTCCTCCAAACGTGGTACAAGGGTTGTTACATCCTCCAGGTACCTTAAGGGCGC GAGGACATTCACCATTTATATTGGCCGTGCAATGAATTGCGTGACATTTTCCTCCACTAGGTTTTGTTGG GGCAAAAGTCATTGGAATATTAAATCCATCGACTAAAGAAATGTCCCAAAAATCTAAGTTGCTAAACTGG TCCAAGGCGTACTCGGCTAGGGTGTTTGGGGGTTTGCCCCATCCGGTACACTGTAAGACTCCACCACAAT CACCAGTCTGACATGTACCTCTGCCTGCAGCATTAAAGTTGCAACCAGTACGACCCCATATACGTGCCAT CTTAGTTCCCCTCGGAGCATTGATGACCCATGTTTGGCCTCGATTGAGACGTCGACCACCGCCTATCGGA GTCGATGCCGCCCAAACGGTGTATGGACAGTTGTTGCGTACCTCAATAGTGGCAGCATAAGTATAAGTCA CACAAAGAAGGAAGAAGAGAACAAAAGAAGATGTCAAGTAGCCCATGTTTGTTGAAATGTATATATGAGG ATAACTTATTTGAAAGCACTTTATATAGGGGTGTGGCGGCTTTTGGCACTATGTATATTAATCATAAATA ACAATATCATATTTGACAAAAAACGAATAATATTAAAATACGATATAGTAAAGTGGAGGTGAAAAAAGAA AAAATGTATAAGAGCCGCCTAATAAATAATAATTTTATAAATATAGTACAAGTTCACTTTATTTGGTGAC AACTTTGTAACGTGACGGAACTTATTTCTCAAAATAAATATTATTTAAAAAAAAAAAACAGCATCTTTCG TGACCAAAGTTTCTTTCTATATTTATCAATTCCATTTTTTTTACTTTGACAGTATTTTGTATACGTTTAA ACATAAAAATTAGGTTTTTCATATTACTCGTATGGATCGATTAATTTCAAATATACAAATCAATTCGATT AACTATTATATACAGTGGAAATGATGTTTATGTGACTCGTAATTGATATTTTAAGCGATCAGAATATTTT AATGCATATCTATTAAGAAAATACCTATTGATGCAAAAGTCAACAAAATTATTTAATTTATTTATTTTAC GATCGAAGCATGAATACATTTACTAATTAAAGTAAATTGGGAGGAATTGATGGACGAGTCATCAAGCTTA TCAAATGTCAATCAACATTAAAATAATGTTAGTTTATGGCTGCTTTTAAATGAACAAGTGGATCATGTAC AATTGAGTAAAAAATATCTCCTATAAATATATATATCCCTCTAAAACCAAATAACATAACACACAAATAT AAACTTTAGATTCTTAAAAACAAAGCTGAATTATATGGAGGCAAATAAGTCTATGGTGAAGTTGGTTGCT TTCTTGATAATTCTTGTATCATCATGTAATTAATTGACCATTTATTTCTACTTTATTAAATTTATTCACA TTTCTTATTTTTATTTGTTAAAAGAAATTGATTAATTATGTAAATTATTTATGAACAGGCTTTCAATCTC TCACTGCTCAAGATTTGGAAATCGAAGTTAGCGATGGCTTAAATGTATTGCAAGTACATGATGTGTCTCA ATCTTTTTGTCCAGGTTTGGTTTTTATCTCTACCGTCCTTTTTGGATATATATACACGTACGACGTCCAA CTAAATCTAAATAAATTTTTGGATCGAATCTTAGGTCGATTATCAGGGTAATATCTATAGATTCGAGTGT TGGGGTTGAGGTCATATCCCAATTTAAATGTCATGTTTCGAGTTGGGAGTTAGGGTCAGGTACTAGGTTA AATCCCATGCTCGGATACTGAGTCTGTTGTCGAGTTATGTCCTAATTCACGTGTCGAAATCGAGTTCCGA ATGAATTTTCGATCAGAAACTATTTTCCTACTCTCTAGCCAAATATAAAAGATATTTTCTAAAAAATATT TTCCTTCGTAGCAAACACACCATAATTAATTATTTTTTTCTCATATAAAATAACTTTTGATTCATAGTTT TTTAGTCAATGCTCTTTTATAAATAATTAGTTGTATTTTTTTTTTATAAATTATAGGTGTGACAAAGGAA AGTTGGCCAGAACTTCTTGGAACACCAGCTAAGTTTGCAAAGCAAATAATTCAAAAGGAAAATCCAAAAT TAACAAATGTTGAAACTCTACTGAATGGTTCAGCTTTTACAGAAGATTTGAGATGCAATAGAGTTCGTCT TTTTGTAAATTTATTGGACATTGTTGTACAAACTCCCAAAGTTGGTTGAACAAAATTAATTGATGTTATA TCATATGTATCTAGCCTCCACAAAAATAAATTGGAGATGTATGGTTAAAATTTCCACTATATTTGGTGAT AAATAAATGTGGCTTTTAATATTAATATGATTTTTGTTATATATACAATTACTTCTACTATATACCAGTG AAATCTCATTAGCATTGGGAAGGTGTTACGTACGCAACCTTATCTTATGCTAACTATCATCAGGGCGGTC TTAACGCTATGCAACTAAAGGGGGGTGGGGGGTATTTCTAAGAAAAAATTAATTATAAATGTTATTTTAA AAATAATTATATGTACTATGAGTGGTATGATTTCTACAAGGAAATTGCATATATAATGAGTATATCATCT CAATTGTGACTTTGATGAAAAGTTCTGACTTTTAAGAAAATTTTGTCTTTTATGAAAGATAGTGACATTT GTTTTATCGAAAAAGGGTGGTGATTTTCGAAAAGAGTTTGTTTTTAGACTTTTAGGAGTCGCCACTTAAT TTTTAAGAAAAATCAAGAAAACTTCGTTTCTAAACAACTTAAACAGAAAAAATTGTTTTGAAACATTTTA AGGGTTCGGGATTCCTATTAGTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCCGTTAACTTACGGTT TTCCAATGATTAACTATTTGATTATTTTTATTTTTAACCTTTGCAAATTTATTTGAACTTTTAACATGAC TTACTTAGCCAAACTTAGCCAATTTTGAAATATTTATTGTTTATCTTGCCAGTTTACGAAACCATTTCAA AGTTAAGCTTACTCAAGGTTTTATCTAAACAAGCCTTACGAATTTGAAACAATTTATCATTTAGAAGTTA GTTTTAAAAATTTTTAGGTTTGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGTTTCCTAATCCTAAG CTAACGGCATTGAAAACAAAATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAGAGAGAGATTTGGGT CCAACTCCGGGTTCTGTTATCCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTTTTTTAGCCCATTTC TGTACCTGTCCTAATCTAAACATACAAACATAATATAGAAAACAAAAAAACGACAAGAGGGCTTAGGCCC ATTACAAAATATGCAAAGTAAAATAAAACGAAAGAAAAGTCTTTTAGTCCATCGTCTTCAATCTCCATGA CCCTTGTCTTTCTACACTTGCTCGTTTTGATGAATTGACTCGATAAAGTTCAGAGTCTTCAAGACTCTCT
Page | 37
GEN 3040
CGAAATGCGAGGAGGATAATGGGATTCCCATAATGGACTCACGGGGAGTTTTCACATGTCACAAAAAAAA CGATAAAATGAAGATTGGCATTTTTAAAACTACAATTAGAATAAGCAAGGTTCTAAAGCATAACAAGGAT ATCTTTATAATGCAATACTAATTAAATCTTCATGAGAAGCAAAGCCAACATCCAACAACCTTAATGGTAC TTAGAGATATGCAAATCAGTCAACCTAAATGACACTACATAACGATACGTAAACTGTGCGGCGAAACCCC AAGTGTAGTGAAACCTTCACACAAAATTGTTGGTAATTTCCAAATTTTATATTTAAAGAAAGGACTTAGC ATGGGATAAATAACGAATACAAATAGAGATGCATCATAAATTGAGTATCAATTAGCCGTTTTGCGCTTCA AACATTTGAAACAAACTTGAACCAATCATAAGAGGACAGCCTCATCAAATAAAACAGGATCCCGATCACC TTATCACCCAAACATCTAACACACTAAGCGAAAGGACCATTCAATCGACCTACTACATGGCTGTGACCTC GGACAGCTAACACAAGTGGCCACCAAGATAAAGATGGCCTTTCAAACCACAGATTTCCAAAATCCACACC ACCCATACGGCATACAAAACGAAACTTTCATGCAAGACGCCTGACAAACATGATATCTAAACAAGATAAC AAGTTTGGGAGATAAATACATTATAGAGAATAAACAACCCTCATATATTCTTAAAGCGATACTCGAACCA AAAGCTTGAAGAAGGTTCGATTGAAAAGAAGCTTACAACTACAACTCCCGCGAGATAGACATCATGAGCA AGCTTGGACGAGACAACAAGGGAAAATTACACTCTAAACATACATACATGCATACAAACAATCCAAGAGC CTGCTTTAACGAATAGACATCGATACTCGGATTAATGAGTCATTTCACAGAAGCTCTTGCCAATATGTCT TAACAATCTCATTCCAATCATGTGACGAATAAATAGTGTTCTACGAGATTAAGCTAGCATATATTGAAAG TTCTAAATGGCAAGCAAAGCAGCGACAGTGTTAACAAACCACAAAGACAAGCTCAACCGACCATGTATAT TCACATTTTCGAGTATCTCAACATGTTCGACCACAAACTAAGAAAGGGCCAGCAAGTCGATTAACAAACA TCACATGTCATACGGCGAAGGCTCCTCGAAACAAATACAAAGTCATGACTAACTCGTGAAGACAAACAGA GAAGCAATGTCGACGAACCATCACATTGGGACAGAATCACAACGTAAGACATATTCCAGACTTAAACAAC CAAACGAAATATGATACCTCATGTTGACAGCACCACAAACCACGACTCAACTCCATAAAGAAAATTTAAA ATAATAAAGAAAAATGGGACATGTACCTTTCTCGGAGCAGCGGAACAGGGATGACGGCGAGCAGACGACG ATGACTTCCCCACGCGACCCACGGCTAGACGTTTATCTCGTTTGTTCTTCGTATTGGAGTCCTATTCCCG AAAAATCCCGACTGTTTTGCTACACGAACAGGACAGGAAGAAATCTTCTTCAAAGTCTTCCTAAACCAGA ATTTCTAACCAAATTTTCTAACTTCTTTTGGTTATCTCAGAAACAATGGAATAAACCCAGAATTTCAAAC AACGTTCCCAACCCAAAATTTCCTAACAAGAGACGGAAGAAGCAGAGTCTTTTTTTTTCGAAAATTTTCT CAACCAAAATCCCTAACCCTTTTCTGTGTCTTTCTTTTCTTTTTCAAAAATCTCAAATATCTAACATTCT TTCAACTCCCAAAATTCCCAGCGCTCCAAGTTCTGCTTTTCCGAATTTTTCGATGAAAAAATCTCTCACC CCTTTTCTGATGAAGAAAAGGGGTATTTATAGAGGAGAAGAAATAGCCCCCCTTTTCTCCTTTTGAAATC GAAACTAAATCCCGTTTCGGTCAAACTTCACATGGCACAGGCGTGTTGGAGAGGATGAAATTGACACCTC GCAGACCTACTCGACAGCGTATGGGTCTCTGCGCTTCCGTTTGGTTCACGCGAAGAGAAAGATGGAAGGA GAAGAGCGTGGGGTTGTTGGAGTCTCTCTGTTTTTCGCGTGATTTCTGGGGAATTATGGGTTTTTGTCGA GAGGGGAAGGAGAGAGCGAGCGTGGGGGAGACGCGGGGAGAGGATAAGAGGAAGGGAGAGAGCGAGGAGA GAGCGTGGGGAGGGGGTCGCGTGGCTGCGCATCTGGGAATTGTTTGGGAATTTTGGCTTTCTGTTTTGCT GGGTTCGCGTGGGGTGAGGGGAGAAGAAGGAAAGGGGAACGGGTCGGGTCGGGTAGGATGGGTTCTGGGT CGGGTAGGATGGGTTATGGTGAATTGGGTTGGGCTGGAGAATAAAAGAATTGGGTGTTGGGATTAATTTG TTGGGCTTGTTTTAAAAATGTGTGTTGGGTTGGTATTTTTTAAAAGAAATAAAAGGGGATGTTGGACTGG GTTGAAGAGGATGGGCGTCTGGGGGGAATTGGGCCTTGAGTCTGTGGGCTGTTGGGAATATTGGGTGTAA GGAATTTAGGCAAAATGGGTTGAGGACGAAAGTGGGCCTCGGAATAAGAGTGGGCTGATGGGTAAAGGAA TGGGTTCAAGATATACATATATACATATACATATATGTATATACATATATGTATATATGTACATATATTT ATTTTCGCAAGGTTTATAAAACTAACTAACTCATACCGACGCGGGTCAAAATTGGGTGTCAACAACTGTC CCTCATTTTACTTGGGTGGATGCAAGCAACTCGAGGAAAATGAAGTTGACCGAACCAATTTTGACCAACT CCACTCGCCTTCAGGAAGAAGGATTTGTCAAAGAGTTTAGGAATTATGGCCGAACCCTTGCAAACGGGTC TCCTACATACCTCAGGCTATATGAGAATTCAGGCCACTTGTAGTTCGATAAGCTTGATTTACCGCACGAT TTCAAAGAATCAAAAGCCACCTTGACACCGAATTATGAAGGTATCGAAATGCTCGAGAATAGAATTCGAG AGCGAATGTTGGAATACAGCTGAGTTTTCAACATTGAATCCGCCTACATACCCTTAAACCTTCGGAATCA GGCTGTGTGTAGTTCGACTCGATCGTTGGAAAAGGAAAACTATTATGCTAGCTAACCTTCGGTTTTGGAC GAGTGACACATTAAACGAGTATGAAAAAGAGGCTTGTAACCTCTTAGCCATGAATGTGGCGCGCTTCACA TCCATAATTAAGAACTTACACGGACATAATTTCCAAATCTTTTGGCGTATTGTCACTCAGATTGGAAACT TGCTTCCGATAAAACAAAACTTTCGGAAGCGAGACAGAAATTGACAGGACTAGAGAAAACTCGTATGCCT CGAGAGGGTGTTTCGATTGTGGTGGAAGTCGCTCCTCTGCTCGCGTCCTAAGAGTTTGACACTCCTCTTT GGACTGTGTCCCAGTTCGCTGCTAAGAAAAAGTTCCACGTTGTGCTGCATCCTTTTTGATGTCGTCCGCA CCTGTGGTTTTTGGAGAATTCATCCTTTTGGATGCTCTCGACAAAGACTGAGATAAAACTCGTCAGCTTA AAAATGCAATTAGGATGGGAGACAGTGTCTTTTACCATGTCGACACTTCATTGTTCTCCTCCATGTTCGA CGTCGACTCGATCGGTCTGACGTCCAGCAAATAAAGCTTATGCCTTGTGTTTTGCAATATCATCTTCAGT GTATTCCATACTTGCTGTCAAAATAAATAAATAAATGCTGATGCGATATTGATGTTTCTTTTGTTGTCAT CTTCGATGCTCTCCATGATGTTTATTTTTATAAGAAATAACATAATGCAGTCGAGGCCAATGGATAAGTA TTGCTCTTTCTTTATCCAAGTACGTCTTCTTGCGGGTCATGAATATCTTCCCGCGATACGTAAATTCTCG
Page | 38
GEN 3040
CGAGATATGAATCTTCTCGCGATGCATAACTTTCAGCGAAGAATAAAATCTTCTTGCGATGTGCAAACTT TGACGAGGCATGAATTCTTCTCGTCGTGCATAAATTTTGACGAGGCATGAAATCTTCTCGTCGTGCATAA TTATTGACTCGCGATGCATGATTTCCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAA TTCTTCTCGCGATGTATAAATTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGA TGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAG GCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCGCGATGCATGAATTCCAGC GAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCGTGAAATCTTCTCGTCATGCATGATTAT TGACTCGCGATGCATGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAA TTTTGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTTCCGCGATGCA TGATTCCGCGATGCATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTTGACGAGGCAT GAAATCTTCTCGTCATGCATGATTATTGACTCGCGATGCATGATTCCCGCGATGCATGATTCCGCGATGC ATGAATTCCAGCGAGGCATAAATTCTTCTCGCGATGTATAAATTTCGACGAGGCATGAAATCTTCTCGTC ATGCATGATTATTGACTCGCGATGCATGATTTTCGCGATGCATGACTCCGCGATGCATGAATTCCAGCGA GGCATAAATTCTTCTCGCGATGTATAAATTTCGACGAGGCATGAAATCTTCTCGTCATGCATGATTATTT ATATTGCCCCCGTGAATAATAACGGCAAGACGACCTCCAAATAATATATCGACTTGATCGATAATGATAA AAATAATAATTGCATAGCTGTCTCTTCTGAGGTAATGCATTGTCTTGATCGACAATAATCATCTTGCTTT GATAACGCGATGCAAATAGCGTCTTCTATAGAAATCGTGAAATCTTCGCTAAGATTCCTTTTTGATCCAC CTTTGAATCTGGCCAGGCACTTTGTAAATTTCATGTATTGGGGAACGGCTTGAAATAAACAAACACATTC ACAAGCATCTTGGCATAAAGATATGAAATGCTTCTTGCTTTCAATAAAATCACCGGCTTTGGAAATAGTT TTCGCCTTCTCCGTATTCATCAGTCGGAAGAATTCGGCCGATTTCGAAGTGAAGCTATAAACCTGCATCC ACAGAAAAATTGTCAGTTTTAAACATACTGATCTGTGTCGATCCCTTCGATTGTTTGCTCCTTGTCTTGA CGTCCTCGGTTGATTTCCCGATTTCTCGACTCTTGATAGATAAGAAAATATCTTATGCCAAAACAGATGA AATATAAAAGGGAAATTTTTAAGAGAAGCAAGAAATTTTTTAAGAAATAGTATCTCGAAAAAGTCAATGC CAAGTCATGTCATGTCAGGCTTAACGAGCTTCTTTGAGTCCGACGCTTGTCGAATGAATTTCAGAGGCAT TCCAGACTTGTGGGGAAGTCCCATGACGAAATTTTGAGGCCATCCTCAAAATTTCTGTCCCAGTTTAGTA TCTTGTTCATCTTTCCATTGTAACCGAACAGATCTCGGAATTTTTGAGATCTTCTAAAAAATTCTGTCCC AGTCGCAAATTCGAAATGTCTTTAGTTTGATCCGCTGAAGAGAAGGTTCCTTCTGGAGAATTTTTGAGTC CCTCTCAAAATTCTGTCCCAGTTTCTATTGTAGAAAGGGGGGAATAGAATTTACGAGATATATGACCGAG CCCTTGTGGCGCCTACGTATCCCGTTGAGGCAGGAATCAGGTCAAACGTAGTTCCCCTTTAAAGGTTAAC AAGAATAAAATTTACAAAGAAACCGACCGAAGCCGACAAAGGCCGCCTACGTATCTCATTCTTGAGAATT CAGGTCGAACGTAGTTCAGGTCGTAGAATTTGGTCTTCTGTTTTGACTTGTTAAAGAAGTCTTACTTCAA ATGAAATTTTGAGATCCTCTCAAAAATTCTGTCCCAGTTTCCATTATGGAGAGAAATGGAAATCTTCTGG GTATATGACCGAGCCGTTGTGGCGCCTACGTATCCCGTTGAGGCAGGAATCAGGTCAAACGTAGTTCTAA CGTAAGGAATAATCAAGGGGAATAAATGGGGTGACCGAAGCCGACATAGGCCGCCTACGTATCTCATTCT CGAGAATTCAGGTCAGACGTAGTTCGTTACAAGAGGGATAAGAATTCAAATTCGAACCAATACAAGCAAA CAGAAGATTACAAGTAAATGGTGGGAAATGCAAAACGAACTTCACACGTAATATCTCTTGACAGCATCTG AGTTGATAGGTTTGGGCCATACAGCGCCATCCATCTCTGACAAGACTAAAGCACCTCCAGATAGTACTTT GCGGACCATGTAAGGACCTTGCCAGTTTGGTGCGAACTTTCCTTTGTACTCGTCTTGATGAGGAAAAATA CGCTTAAGAACCAACTGACCAACTTTAAAATTTCTGGCTCTTACTCTTTTGTGAAAAGCGCGAGTCATTC TTTGTCTATACAACTGGCCATGGCAAACGGCAACCATCCTCTTTTCATCAATCAAGGCTAGTTGATCAAT CCGTTTGCTAACCCACTCAGCATTACTTAGCTCAGCTTCTTGGATGATCCTCAGTGACGGTATCTCGACT TCGACAGGTACGACTGCTTCTGTTCCGTATACTAGCAAGTATGGAGTAGCCCCAGTTGATGTTCTGACCG TCGTTCGATAACCCAGTAGAGCATATGGCAACATTTCATGCCAACCCCGCTGCTTGTCAATCATTTTCCC CAGAATCTTCTTGATGTTCTTGTTGGCGGCCTCTACAGCTCCGTTCATTTGAGGGCGATAAGCAGTTGAC CTTCGGTGAATAATCTTAAATTGTTCACATATCTCTTTCATCAGATGACTGTTGAGATTTGCACCGTTAT CAGTGATGATGGATTCTGGAACTCCAAATCGGCAGATCAGATTGTTGCGGACAAAGTCGGCCACCACTTT CTTGGTTACCGATTTGTAAGAGGCTGCTTCCACCCATTTGGTGAAATAATCGATGGCAACCAAAATAAAT CTGTGTCCATTGGAAGCGACCGGTTCTATAGGGCCGATGACATCCATTCCCCAAGCTACAAATGGCCAAG GTGAGCTCATAGCATTGAGTTCGTGAGGTGGCACCCTTATCAAATCTCCGTGCACTTGACATTTATGGCA TTTTTGCACGAATTTGCAACAGTCGTTCTCCATAGTCATCCAGAAGTAACCGGCTCGAAGGACTTTTCTT GCCAAGGTAAGCCCGTTCATATGTGTGCCACAGACTCCAGCATGTATCTGTTCAATAAGCCTCACGGCTT CAGCAGCATCCACGCATCTCAAAAGACCCAAATCTGGAGTCCTCCTGTAAAGGACTTCTCCATTCAAAAA GAAATTGAGAGCCATACGACGTATCGACTTCTTTTGATTAGATGTGGCGTCTTCTGGATATGTCCCAGAC TCCAAGTACCTCTTTATGTCGAAATACCAAGGCAAACCATCTGGTTCTGATTCCACGTGTGAACAATGGA CTGGATGCTCCTTCAAATCTATATCCAGCGGGTCGATGTAATCAATATCCGGATGTTTGATCATTGAAGC GATGGTGGCAAGAGCATCAGCTAATTCATTCTGTATTCGGGGAGTATGTCTGAACTCGATCTTGCGAAAC CTTTTACACAGGTTTTGCACATATTGTACATAAGGTACAATCTTTGGGTTCTTCACAGCCCATTCTCCTT
Page | 39
GEN 3040
GAACTTGATGGATCAAGAGGTCTGAATCTCCAATAACCAGTAACTCGTAAACATTCATGTCAACGGCCAT TTTCAAACCGAGAATGCAAGCTTCATACTCAGCCATGTTGTTTGTGCAGTTGAACCGTAGCTTAGCCGCC ATAGGATAGTGCTGACCAGATTCTGATACCAAGACTGCTCCAACACCTTTACCCTGGTGATTCACCGCTC CATCAAAGAATAATCTCCAACCTGGATAAACTTCAGAGATATCTTCACCCACAAATGACACTTCTTCATC GTGAAAATATGTCTTGAGAGGTTCATACTCTTCATCAACGGGATTTTCCGCAAGATGATCAGCCAAAGCT TGTGCTTTTATTGCCTTCTGAGTCACGTACACGATGTCAAACTCACTCAACAGCATTTGCCATTTAGCCA GCTTCCCGGTCGGCATCGCTTTCCGGAAAATATACTTCAATGGATCCATTCTGGAGATAAGATATGTAGT ATACGACGACAAATAGTGCCTCAGCTTCTGGGCAAGCCATGTCAGAGCACAGCATGTTCTTTCCAGCAAA GTGTAACGAGACTCGTACGGAGTAAACTTCTTGCTGATGTAGTAGATAGCTTTTTCCTTCTTCCCTGTCT CGTCGTGTTGACCAAGTACACATCCAAAGACGTTATCCGAGACAGACAAATATAGCAACAAAGGACTCCC TTCTCGCGGAGGAACCAATACTGGTGGGTTAGACAAGTAGCTCTTGATAGCGTCGAAAGCGGTCTGGCAC TCCTCAGTCCACTTAGTTGGGGCATCTTTCTTCAGCAACTTGAAGATAGGCTCACATACCACCGTCGATT GTGCTATAAAACGGCTGATATAGTTTAACCTCCCTAAGAAGCTCATCACCTCTTTTCTCGTCTTCGGCGG AGGTAACTCCTGAATTGCTTTGATCTTGGAAGGGTCGAGCTCAATACCTCTTCTGCTGACTATAAACCCT AACAATTTCCCAGCTGGGACTCCAAAAGCACACTTGGCGGGATTTAGCTTCAAGTTGTACCTACGCAAAC GTTCAAAGAATTTTCGCAGGTGTGTCAAATGATCCGAACTCTCGCGGGATTTAATTATGACGTCGTCCAC GTACACTTCAATTTCCTTGTGCATCATGTCGTGGAAGATAGTCGTCATGGCTCTCATGTAAGTGGCACCG GCGTTTTTGAGCCCAAACGGCATCACCCTGTAGTGATATACCCCCCAAGGTGTGATGAAGGCCGTTTTCT CCGCGTCTTCTTCATCCATCAGAATCTGGTGATAACCCGCGTAACAATCCACAAATGACTGCATCTCATG TTTCGCACAGTTATCAATCAGAATATGGATATTTGGCAACGGGAAATTATCCTTTGGACTAGCCTTGTTG AGATCTCTGTAATCAACGCAAATCCTGATTTTTCCATCTTTCTTGGCGACCGGAACGACATTCGCCAACC AGGTGGGATATTGCGTTACTTCTACCAACCGGGACTCTATCTGCTTGGTGATTTCCTCCTTAATCTTCAA ACTCAACTCAGGCTTGAATTTCCGAGTCTTTTGCTTCACTGGCTCGAACCCTGGGTTGATAGGCAGCTTA TGAGATACGACATCGGTACTCAGCCCCTGCATGTCACTGACCTTCCAAACAAACACATCGCTGTATTCGG CAAGCAAATGAATCAGGCTCTCCTTCTGAGTTTCATTCAGGTGAGTGCTGATCTTAACCTCTTTGACACA TTCTGAATCTCCCAAATTTACCGTCTCTGTTTCCTCCAGATTCGGTTTATGTTGATTCTCGAACTGTCGA AACTCTTCTACTACATAGTCTGGTTCCCCACTTTCTTCATCGTAGTCGTCAGCCTCATCGTCATTTACCT CATTTTGTTCGTTTAGCTCATGACATGACATGACGTTGGCAGGTTTGTAACTTACGTTACTGAAATCATA AACAGACAAAATTAGAAAAGTAAAACATAACAAGCAATCGAATAAACAAAGTCAAAATAAGGAGGCTTTC ATTTAAATTGAATCAAGATGCAAAGTTGGGTCATGGCACAAGGCCACGTCGCCCTTTAAACAATCATAAC AAAATTCAAAATCAAGAAAATGCAGAAATGGTGGGTCTCGGGCCTCTTCCGAACGACCACCGATTTTGGC ATGCACAAAATAATTCCTTTCTACCCAAAAGTCCGGGACATCAGGATTGGTGTGGACGTCCAATTCTTCA GCATCTCCCCCGGTTCAGCATCGCGAAAGCCAGCTGGCTCGGCCTCCTCCTCTATGACGGCATTGATCTC CTTGAACAGGTCACAGATTCCTTCCCCGTCGTCTTCAGGCTCGGCATGCTCCCGAATTGGAAAGGAGTGA TAGAGATGCGGGATCGGCTTAGTCAACTCTTGATCCCTTCTCCTCTTCATCTTCATATCATCATCTGTGG GGATGTACCCCAAACCATACTTTGATCCCTGAGCAAGGACCGGAACAGGCTCAATAATTCCTTGGGAATC TCTTCCCAATCCGAAACCTGGCTCGAACCCATTTTGCAACATCACCGTGGCTATCATTTTGTACACGGTT GGCATGGAGTTGAGGGCCAAATCTTCGTTGGTGGCATTCACCAGCTCCACCGTGTAAAAGTCTGTGCCTT GCGGCATCTCATCAATGACCGACACCTGCTTGCCCGAGCGACTTCCCTCGCCGTGAATCACTAACTCTTC ATTTTTCCACACAAGCTTCATCATCTGATGGAGAGTAGAGGGGACGGCTCCAGCCATGTGGATAAACGGC CTTCCCAAAAGAAGGTTGTAGCTAGTGTCTATGTCCAATACTTGGAATTGCGCACTGAACTCTGCGGGGC CCATTTGAAGGGTCAAAGTCACAGCCCCCAAGGTGTCTCTTTGCACACCATCAAATGCCCTTACATTGAC CTGATTCTGCTCCAGTTTCCCAAGGTCAAAATTTAGTTGCCTCAACGTCGTCAATGGGCAAATATTCAAA CCGGACCCATCATCTACCAAAACACAGTTGACAACCTTTCCACGACATATCACGGTAATGTGTAGCGCTT TGTTGTGGGATCTTCCTTCGACTGGCAACTCATCGTCACAAAAGCTGATCCGGTGCCCCCGAATGACTTG ATGAATCATAGAAGCCACGTTATCACTACTTGTACCTGAGGGTACGTATGTATCATCAAGAGCTTTCATT AAGGCCTGCCTGTGGGACTGAGAGCTCATCAGCAAGGCCCACACGGAGATCTGAGCCGGAGTCTTCTCTA AATGTTTGACGATGGAATAGTCCTTCGGTTGCATCCTTCTCCAGAATTCTTCTGCTTCCCCCTCGCTTAT CGGCCTCTTAGCATGGTCCTTCTTCTGTCCTCCGAAGAAACCTCATCAGGAGTGTAGCACCTTCCGGACC TGGTCATGCCTTGGGCCACGGCAGTTTCAATGACAAATTTGGGCTTAACGAGGGTTTTCGAAGGCACCAA GGCAACAGCCTTTGCAGGTGTCAGAATAACAAACTTCCTCCTCTCTTTGACACTTAAAGAAGCGACAGCC CTTTCCAAGTCCTCATTCATAATAGGGGTAATCATCTTTGTTCCACACCCGTCTTCATCCGTTTCAATCA TATTAAGGTTGTCACCTCCATGATTCGGCAACGGATTTGTGTTGACGTTTGGCACCGCCGGTTGAAGAGA AACTACCTCCTGATCGATCAGGTCTTGGATTTTGTGCTTAAGGTTGATGCAATCTTCCGTGTCATGTCCA ACACGATTGGAATGATAAGCACACCTTTGATCTGGCCTGTAGAACTTTGAGTTGACATCCCCGGGTTTGG GCCCCACAGGGTGGATGTATCCATCTGCGGCTAGCCTCTCAAATAGTTTGGTCCGGCTTTCAGCAAGTAT GGTAAAGTTCCTTGAAGGCCTTTTTTCAGATCTCGGACGGGAAGTATCATAATTGCTTTGCCGAGGGGGA
Page | 40
GEN 3040
GGCACCCGCTGATAATTTTGGGTATTTGTACGAGGGGCTTGGCTCCGGGGATAAGGGTTTGCCTGGAAAT TTGGCATTGGAGCTTGGTAATTCGGGAGGGGGTCCTGGTATAATGGAGCTTGGACTTGATAAGTGGGCGG AGGTGGTCGGTAGCTCGACTGTACGTTGGCACAGTTGGGAGCAATATTCTGATAAGGGGATGGGATCTGG TATGGTTGAGGGTAATTTGGGAATATGGGAGAAAAATTTTGGAGATTAGAGGGTGGACCTTAGTACGACG AAATTTGAGCATTCTGATAGGTGGGGATAGTATTGTGGTTATTAGGATGATTGGATTGTGGGTAGTAGGA TCGGTGAGACTTTGGGGAAGGCCTGGAACGATCTTGGGAATGTGACGGGTTTTTAGGGGTTTTTCTTCCC CCATACGAGACAGCGGCAACTTCCTCTCTTCGCTTTCTTATCAATCCTGAAGACCCAGGCGACGCAGATA CACGGGCTATCTTCCCCGATTTTAGACCATCTTTGATAGTCTCACCGACTTTGACTATCTCAGCGAATTT AGCTCCGACCAGCAACATGATTCTATCATAGTACTCAGGCTCCTGTACCCGCACGAACACTTCCACAATC TCTTTCTCGGTCATGGGTGGCCTTACCCTCGCCGCTTCTTTCCTCCACCTATAGGCAAATTCCCTATAGC TTTCAGTTGGTTTCTGCTTCATCTTCTCTAGAGAATACCGATCGGGCACTATTTCAACATTGTAGGCGAA TCGGTCAATGAAGTCCTTAGCCAATGCATTCCAACTGGGCCACTGCCTGGTTTCATGTGAAGTAAACCAC TCGAGGGCCTCTCCACACAGACTTCGGCTGAAAAGCCGCATCAACAAGGCCTCATCCCTGCCAACTCCCA CGAGCTGGTCACAATACGCTCTCAAATGCGCCATAGGATTGCCCACTCCTCCGAAGGTGTCAAACTTCGG AATTTTAAAACCTTCGGGAAGGTTCAAATCTGGATGGATGCACAATTCTGCGTAGCTAAGTCCGACGGCG TCTGGGATGCACTGCAGCTCTTTCATAGCCCTTTTGATTTCTTCCTTTATATCGATCTTCACTTCTTCTT TCGCCTTCCATTCTCTTTCCTGTTCTTCATAGTGATCCAACTCAGAACCGTGCACCTCGTGCTCGGCAGG AACGGGAACTTGGAAGGTGGCTCTTTTTGGTAGGGGTGGAGCTATAGAGGGACTTGGAACGTTTTGGACG GTCTGGTAGTTTTGTGGGTAGGCCTGAGGATTGGTGTTCTGACTCAAATGCGGATGAAATGCTTGGGTAT TGAAGGCAGTTTGAGTTTGGTTTTGATTTTGTGGCGGTGGGGCAGTTTGAGTATTCTGAGGATGGGGTGG TATTTGAGGGTGGTTATTTGGAAGAGGTGAAGGAGTTTGGTAAGATGCAGAAGCGTATTGAGGGTTTTGG GTTGTGAGGTCAATCACAGACGGATTATGCACAGGTGTAGAAGGCTGGTTCTGAGTTGGATCTATGTTTG ATGAAAGGAAGTAGACCGGAGGTCTTGCATCAGCAATATTAGTACCAAATCCAGGTGGAGGCGCATCCTG TCTCCGTTGCATTTCAACTTTCATTTCAGCAATCTGTTGCATCAGTTGCATGATCAATTCGTTCTGTTCC ACTACAGTGGGCTGAGCCACCACAACGTCTGTAAGACGCCCTTCGTCATTGTTGTCTCCCATAACTATTT TTCCTTTGTCTGAATTTGATCGAGGGAAGGAATCTGTAGGACCTTTTGACCTGGTGAAGTAAGAATGATC TGCCAGCTTTATCGACACAGATCAGTAAAAGGTACCTGAGAGGTAGAAACAACTCAAAGGCAAATTTGTT AGTGCATATCCAGAGTACAAAATGCATAATATCGCACATAAAACAGATAAGCACACAAGTCGTTTTGTTT GAGGATATCTTAACCCACGAATAAGGACGGATATATATTTTGAACAAACAGGAATTTGTTTGTTTGGAGA TATCTTGGGAAATCTGAATCACGTTGAGCTATGGTCTTCTTGCAGCTGCTTTGCGACGTCTGTTGATCTT ATCTTCGTATCTCCGAAACATGGAGGAGAATGAAAATTTTCTGTGATAGGGGCCGGGCCGGGAAGGCTGC CTACGTATCTCACGGGGAGAATTCAGGCCCAACGTAGTTCGGATTTTAGGATGAAAATTTTCCATTCATT CTTTCGTTGTGATTACAAGGGAATTGAAAAACAACATTGAGATTTCTAGAAGACCACATTTGGAGATTTC TTGTCTTGTGGCTGCGCCATTCCTTTCCTTTCATGCAGTCGAAAATGGAGGCTTGTAGACGATTTCTTCT TCATCATCCTCCTCAATCACTTCATGGCCGGGGCCACTGTTGCCTGTTCCCTGATCACGGGCGAGGTATT TTCCGCCCTTTGGGTCGCTATGGGTTGCCAATTCCTCGTCGAGCGCCGTGCTTCTGTCCAACAACACAGC AGTCTCGATATCTATCTTCGCCTCTCTCGCCTTTCCTTCGTGTATCTCCAAACGAAGCAAGTTCAACCTT TTCCTTAGGCTTTCGGTTTCCATCTCAACCTCCTTCTTTCTCTTTATCTGTGACGCCAGATCTTCATCCA ATGTTGTGACCGCAGCTTTGTAGATCGCCGTGGTCGCGTCTACTTTGAGTCCTTCTTCCTTAACCTTCTG AATCTCTCGAGTGACTCGCTCGATCTTTCTTTGCAATTCCTCCTCAGTGAGCTCCGTCTGGACGTTCTTG CCTTTGTCCATAGCGGTGATTCGACTTTCCTGAGATGATAGAGCGGTATTTAGGATTTATGGTATGGATT TTGTGTGAGAAACTTGGGACTCGGGAATTTTGGTCGGACATTTGTAATGTTGGCTTCTGTATACTCTTTT GAATTTTGGCTAGGAGTGGGTTTACGATATCCTCAATCAAAGCAACATAACATATAAACATTTAAACAAA TATATCGTGTCCTAAACATGCATGTGACCCTTTTGTGCCAAGGGTAGGCCTAGCGATCTGAAGGATGTAT TGCGCCATTTAAATAAGTAATTGTATCCCCGAAACAAATTTCACTAGATGTCATAGCGTTCATTACACAA TCAAAATTAAACGGGATATATCTTGGGAAGGGGATGTAGTGCAAATACACATATAAAATCAATCCCACGC AGGGGAGTTAAAAGGTCGATGATCCAGCTCCTCTTCTGGCCTTCTCCACTTCTTCTCGGATAATGCATTT GCTGGTGAACACGTATGGCTCCGCGAACGCAGCTTTGGAAGATGTTGGTACGGCTTGTAAGGTGTTCATC TGCTCGTCCAAAATAGTGTCCAAACGCATTAGACAAGTCCTTGTGTCAGCTAATTCTTGTGACATTACGG CTAAAACTCTCTGATTTTCTAATTCCCGGAGTTGGTACGCTTGCTCCCTTTGTTGAAATTCCTGTTCTTT CTTATAGAATTCCTGCTCCCTCCGGTCCCACTCTTGCTGAAAATGGTACGCGTGAATCTGAAGCCTTGTC TCCACGTCTTCGATTTTCCGACTTGTGCGTGGTGTCGGATCCACTACACCATGCAAATTATTATGCAACC ATGTCTTATATTCGTCGACATACCCAGCTCCATACCTATCTTCGGTGACCTCCCACTTCATGTTGACACG TCCGGCCCATTCCTGGAGGATAATTTTAGCGAAAGGTATTTTGTCCTTCCCGTTGTAATCAACAATGAAA GAGCTGGCATCTCCTTGGGCGGGTATGATTTGGACTTTCCCAAATTGCCGCATTACTCGTGTCGGGTTGT AAGGACGCAATCCTCTGATGCCGGGAAGGACTAAAAATGGCAATTTGGCCCCTTGGGCTGCTATCATCTG AGACCTGAAGCTCGGTAATACCCATTGTATGTCTCCTTCTTTCAAATTGTAAAACACCTTTGCCCACGCA
Page | 41
GEN 3040
TCCCTGGTGGTGTGAATTGGAAATTTACGGATACTCAAGTAGAACTCGTAATTATTGAGAGGACTTGTTT GACTTGGTTCGGCCAAACCATGTAGCTGGGGGTTGTCTCTTTTGCATAGATGCTCCGTCAACCACCATTG AAGTAAGATGTTGCAACCTTGGAAAAACGGAAAACCATTCTTGCACAGGCTCAAAGATCGGTAAATGTCG GACAGGATGACTGGGGCCAAACAGCAATATTTCTTTTCTTCAGATTTTCTACCGACTCCTCTGAACATGG CATTCACTACCATTATTACCCGGGTGTCGATCTCTTTTCCTTCGTCTTTAGGGAACACCATAGTTCCCAA GAGGCATGCGGAGAAAGCGATAGCTTGAGTTTGCCTCCAAGTACTGTGATCATGGAATTCGTCCGGGAAC AATCTAAAATAATTGTCGTGCCCCCACCTCTCGAAAAACCTCATCAGTGGAATGTTGTGAGTCTCTAGCC AATTCGCATTCACTAGGGATAATATGTCTCTTAGCTCAGTCTCGGTTGGCCTATCTGGCAGGAGAATGTG ATGATCGGGACGCTTTTTCCTTTTGCCACACGTCCCAATCGAGTCAAGGCAATCTTTGATCTCTTCTAAT GTGGGTTTTATTTCGACATCTCCAAATCGAAAGACCATCTTCTTATCGTCCCAGAATCGGGTGATGACTT CGATAAACGTGGGCCATACCTTGAAGTCCATTATCGAAGGTAGGTGACCCAAGTAAATCGAGATCTCTCT CTTCTGACACTCCTTAAGATTTTCCCACCATACTCGAAGAATGTCGGGTGCCTGGGTGACCATTTTGAAG TGAAGGAACTGACTTGACATCTACAAAAACATACAACTAGTTAATTTTCAATCCCCCCGATACAACAACT CGTTAAACACGCAATACATCCTTCAAATTTAAACAACAAACATGAGCGTCCTATCGAGCCGCAGGCTCTT TGGACTCAAGGTGGACTTTTCTAATGGGCCCGACACTCCTTGGGTGTCGGGTCATCTTAGGCCAATGCGC TATTTTCGGGATTTGGTTTCGCTCTATCCTAGTTTGACTAAGAGTGGGTTGTATTTAGAGTGGAATGGGT AACCCAAGCGGACTACTTGGGCTGGAACAATTAACGATCGTTGACTATCAAGTAATCAACTACACTCGTC AGGGTGCCCCGAGAAGATTTTTGGTTAACGAACGACTGCGAACCCGCATAATCGTCCTTTAGTGGAAATG TAAAAAAGTGTCGTTTGACGGAAGTATGTATGCTATGTATGCAGCAGTTTAAAAATCTAAACAATTTAAA CATTTAAACAGTTAATCACAAATAATCAAATACGTTAATCTTAAGGTTAGAATCCTCCAAGTCCCCAGTG GAGTCGCCATTTCTGTTTTATCGAAAAAGGGTGGTGATTTTCGAAAAGAGTTTGTTTTTAGACTTTTAGG AGTCGCCACTTAATTTTTAAGAAAAATCAAGAAAACTTCGTTTCTAAACAACTTAAACAGAAAAAATTGT TTTGAAACATTTTAAGGGTTCGGGATTCCTATTAGTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCC GTTAACTTACGGTTTTCCAATGATTAACTATTTGATTATTTTTATTTTTAACCTTTGCAAATTTATTTGA ACTTTTAACATGACTTACTTAGCCAAACTTAGCCAATTTTGAAATATTTATTGTTTATCTTGCCAGTTTA CGAAACCATTTCAAAGTTAAGCTTACTCAAGGTTTTATCTAAACAAGCCTTACGAATTTGAAACAATTTA TCATTTAGAAGTTAGTTTTAAAATTTTTTAGGTTTGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGT TTCCTAATCCTAAGCTAACGGCATTGAAAACAAAATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAG AGAGAGATTTGGGTCCAACTCCGGGTTCTGTTATCCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTT TTTTAGCCCATTTCTGTACCTGTCCTAATCTAAACATACAAACATAATATAGAAAACAAAAAAACGACAA GAGGGCTTAGGCCCATTACAAAATATGCAAAGTAAAATAAAACGAAAGAAAAGTCTTTTAGTCCATCGTC TTCAATCTCCATGACCCTTGTCTTTCTACACTTGCTCGTTTTGATGAATTGACTCGATAAAGTTCAGAGT CTTCAAGACTCTCTCGAAATGCGAGGAGGATAATAAGATTCCCATAATGGACTCACGGGGAGTTTTCACA TGTCACAAAAAAAACGATAAAATGAAGATTGGCATTTTTAAAACTACAATTAGAATAAGCAAGGTTCTAA AGCATAACAAGGATATCTTTATAATGCAATACTAATTAAATCTTCATGAGAAGCAAAGCCAACATCCAAC AACCTTAATGGTACTTAGAGATATGCAAATCAGTCAACCTAAATGACACTACATAACGATACATAAACTG TGCGGCGAAACCCCAAGTGTAGTGAAACCTTCACACAAAATTGTTGGTAATTTCCAAATTTTATATTTAA AGAAAGGACTTAGCATGGGATAAATAACGAATACAAATAGAGATGCATCATAAATTGAGTATCAATTAGC CGTTTTGCGCTTCAAACATTTGAAACAAACTTGAACCAATCATAAGAGGACAGCCTCATCAAATAAAACA GGATCCCGATCACCTTATCACCCAAACATCTAACACACTAAGCGAAAGGACCATTCAATCGACCTACTAC ATGGCTGTGACCTCGGACAGCTAACACAAGTGGCCACCAAGATAAAGATGGCCTTTCAAACCACAGATTT CCAAAATCCACACCACCCATACGGCATACAAAACGAAACTTTCATGCAAGACGCCTGACAAACATGATAT CTAAACAAGATAACAAGTTTGGGAGATAAATACATTATAGAGAATAAACAACCCTCATATATTCTTAAAG CGATACTCGAACCAAAAGCTTGAAGAAGGTTCGATTGAAAAGAAGCTTACAACTACAACTCCCGCGAGAT AGACATCATGAGCAAGCTTGGACGAGACAACAAGGGAAAATTACACTCTAAACATACATACATGCATACA AACAATCCAAGAGCCTGCTTTAACGAATAGACATCGATACTCGGATTAATGAGTCATTTCACAGAAGCTC TTGCCAATATGTCTTAACAATCTCATTCCAATCATGTGACGAATAAATAGTGTTCTACGAGATTAAGCTA GCATATATTGAAAGTTCTAAATGGCAAGCAAAGCAGCGACAGTGTTAACAAACCACAAAGACAAGCTCAA CCGACCATGTATATTCACATTTTCGAGTATCTCAACATGTTCGACCACAAACTAAGAAAGGGCCAGCAAG TCGATTAACAAACATCACATGTCATACGGCGAAGGCTCCTCGAAACAAATACAAAGTCATGACTAACTCG TGAAGACAAACAGAGAAGCAATGTCGACGAACCATCACATTGGGACAGAATCACAACGTAAGACATATTC CAGACTTAAACAACCAAACGAAATATGATACCTCATGTTGACAGCACCACAAACCACGACTCAACTCCAT AAAGAAAATTTAAAATAATAAAGAAAAATGGGACATGTACCTTTCTCGGAGCAGCGGAACAGGGATGACG GCGAGCAGACGACGATGACTTCCCCACGCGACCCACGGCTAGACGTTTATCTCGTTTGTTCTTCGTATTG GAGTCCTATTCCCGAAAAATCCCGACTGTTTTGCTACACGAACAGGACAGGAAGAAATCTTCTTCAAAGT CTTCCTAAACCAGAATTTCTAACCAAATTTTCTAACTTCTTTTGGTTATCTCAGAAACAATGGAATAAAC CCAGAATTTCAAACAACGTTCCCAACCCAAAATTTCCTAACAAGAGACGGAAGAAGCAGAGTCTTTTTTT
Page | 42
GEN 3040
TTCGAAAATTTTCTCAACCAAAATCCCTAACCCTTTTCTGTGTCTTTCTTTTCTTTTTCAAAAATCTCAA ATATCTAACATTCTTTCAACTCCCAAAATTCCCAGCGCTCCAAGTTCTGCTTTTCCGAATTTTTCGATGA AAAAATCTCTCACCCCTTTTCTGATGAAGAAAAGGGGTATTTATAGAGGAGAAGAAATAGCCCCCCTTTT CTCCTTTTGAAATCGAAACTAAATCCCGTTTCGGTCAAACTTCACATGGCACAGGCGTGTTGGAGAGGAT GAAATTGACACCTCGCAGACCTACTCGACAGCGTATGGGTCTCTGCGCTTCCGTTTGGTTCACGCGAAGA GAAAGATGGAAGGAGAAGAGCGTGGGGTTGTTGGAGTCTCTCTGTTTTTCGCGTGATTTCTGGGGAATTA TGGGTTTTTGTCGAGAGGGGAAGGAGAGAGCGAGCGTGGGGGAGACGCGGGGAGAGGATAAGAGGAAGGG AGAGAGCGAGGAGAGAGCGTGGGGAGGGGGTCGCGTGGCTGCGCATCTGGGAATTGTTTGGGAATTTTGG CTTTCTGTTTTGCTGGGTTCGCGTGGGGTGAGGGGAGAAGAAGGAAAGGGCAACGGGTCGGGTCGGGTAG GATGGGTTCTGGGTCGGGTAGGATGGGTTATGGTGAATTGGGTTGGGCTGGAGAATAAAAGAATTGGGTG TTGGGATTAATTTGTTGGGCTTGTTTTAAAAATGTGTGTTGGGTTGGTATTTTTTAAAAGAAATAAAAGG GGATGTTGGACTGGGTTGAAGAGGATGGGCGTCTGGGGGGAATTGGGCCTTGAGTCTGTGGGCTGTTGGG AATATTGGGTGTAAGGAATTTAGGCAAAATGGGTTGAGGACGAAAGTGGGCCTCGGAATAAGAGTGGGCT GATGGGTAAAGGAATGGGTTCAAGATATACATATATACATATACATATATGTATATACATATATGTATAT ATGTACATATATTTATTTTCGCAAGGTTTATAAAACTAACTAACTCATACCGACGCGGGTCAAAATTGGG TGTCAACAACATTTTTAAAGAGTTGTGACTTTTATGAAAAATTGTGACTTTAATAAAGAGTCGCGATTTT TATAAAGGTTGTGACCTTTCTAAAGGAATGCAACTTTTTTAAATAGTTGTAAACTTTTTGATAACACACA AAATACATTTGTTCACACTTCTTTGTTGTCTATAAATAGAGGGATTTTCTTTTATTTTAAAACAACAAAA ATTCTTAAACCTCCTCCTCTTCTTCTTCTTTTTGCACAATTAAATAGTTATGTATTTTGCTCATGTTGAG TGACTCACTGAAACTATTTCTTATGTAACAGATCCTAGTATGTAATTTTATAGTCACTAATTTATGCTTA TGTTATTAAGGATAAATGATAAGATTTGATAATTTTCATTTTCCTTTACATTATTAATATTTGTTATTAC ATAATCTTGTATGTAAGATAATATATATAGTGTTTTAGTTTTAATGATCGATAGATTTTTAGTATTATTT TATAAATTAATGAAATACACGTCTTATTTTTCCCTTATTTTCAATATTCCCTTTTATTTCTAAAAACCTC TAAAATTCCTTATTTATTTAATGTAACTGAGTTACATATTAATGTATCTAAATCAATATTTAATATATTT GAGCTGCATATTATGTATTCGGTTTATTTTATAATGTATCTGAGCTAGTTCTTAATGTATTCGCGCTGCA TATTAATGCATCCAAAAACTAGTATGTAATGTATCCAAGCTACATATTATGTATCCTATTTATGTTATGA TGTACCCGAGCTACATGTTATGTATCCGAGCTACATGTTATGTATCCGATTTATGTTATAATGTATCCGA GATACTCTTTAATGTATCTGAGCTACATATTAATGTATCCGATTTGTGTTACTTTTAAGAGATTAATGTA ATTACAAACTAAAGAGGGATAGATTGTAACTTCACATTAAAACTCTGTGATTTCTAAAATTTATCCAAAA TCTGTAATATTAAATGCACGAATAGTTTTACTAGTATATATATAGCATATCAAACTATGTTTTCACTTTA AGTTGAAAGGGTTTGTGTATATATATTCCACACGCATATATACAAACTTATTATCAATATTATTTTGGTA ATTTTTCATTCATTTTTTTCGCAACCAAAATATTCTTTTTATGGGCAAAAAACTACTTTATAATTAGTTC CTCCAAGACAAGTAAAATATTAGTTTTAGCATCCTTAGGGTAAGCATCATAACACCTTTCCTTAAAAACA CTTACACTGTTGGATCACATTTACCGGAATAACAACAATATTGATCGGTCTTAAAAATCGTACAAGGATT GTTATAACATCCTCTGCGAGTTTTTAGCACTCATGGACAAATCATGTTAATATCAACTCTACACCTAGTG CCACGAGCACGCCCATTCGATATAGGGCTAAATTCTATTGACACGTTGAATCTAGCGATGAAATAGACAT TCAAAAAATTAAAAAAATATATCATCTTAAAACTTCCACTACATAAATTGTGAAAATTTGTAACTCTTTC AATACAGAATTAAATGGATGGAATGATTACAAGGAAAATTTCTATAAGGGAGAAATTTAATAGGTAGCCA CTTATATATTTGATAAGTGTAAGACATCATATTGAAGGAGCAATAGAGAAAATTCATCACATGAAATCAT GATATTAATTATGGCTATATTATATTACATACTGTCATTTAATCTAAATTACCAAACTGCAATGATGATT ACTTCTTTAATTTCTGGAGATTCAAAGTTAATTAAAAAAACTCTGCTAATGAAATTAAGAAAACAGGACT GTTGGTTCATTGGTTAAGTTTGACTATCGATTTAGCGTGTTATAAATAAAAAATACACAAAAACCTAACT GAACCAATCAATAACCACATGGACAATATCCAATACGAGTTATGAACCTGCCAATTTATTAGCACTAGGC ATGTTCAGGGATGGTCGACAGTGTTAAAAAAAGACATGTGTCATAGGCCTTCAAATTTAAATTATAACAG TCGTAAATTATTATTTTTTAAAAAAAGTTTAACATTTCATGAAAAATGAAGAAATCATTAGAAGAATTTT GACATATTTGGAATACTATATCTCATGAAAAATAATTAAAAATAAGAAATGATTATACTATCACATGAAA ACTAGACGAAATCAATTATATAAAAATTATTAACAATTTAAGTTTAAAACCCCATTTAATTTTTACTTTA GAACGTAATACGCTTGAGCCGCCCCCTCGATTTAACCAGGACGTAAAAGTACTAACGATATTATTGTTTA TTCATTATGATAAAACTATATTCAGTTGAAGGGAGACATACATAACACACGTTGGAGATTATTATCACAT TAAGGCAACATAGTTTAGCAGACCGTTTAAGATGCTTATTATTTTGGTAATTTTTTCATACCATTCAATT TGTAGCAAAATATGCTTTATTGTGGCATCAATTATGGGCAAAAAACAACCCTATAATTTGTACTACCACT AGGGCAAGTGAATAAGCTAGTAGGATCATCTTGAGGGTAGCTATACGCATTAGGGCACCTTTGTTTGAAA AATCTCGAAAATTTTGTAGGACCACATGGGCCTTGAGTACAACAATATTGTTGTCCTCCGAACGTGGTAC AAGGGTTGTTACATCCTCCGGGTACCCTAAGTGGACTAGGGCATTCACCGTTTATATTAGCCGTGCAATG AATTGCATGGCATTTTCCTCCACTAGGATTGGTTGGGGCGAAAGTCATCGGTATATTAAATCCGTCAACC AAAGAAATGTCCCAGAAATCTAGGTTGCTGAATTGGTCCAAAGCATATTCAGCTAGGGTGTTTGGGGGTT TGCCCCACCCGGTACAGTGTAGGACCCCACCACAATCACCAGTTTGGCATGAACCTCTACCAGCACCATT
Page | 43
GEN 3040
AAAATTGCAATTAGTACGACCCCATATACGTGCCATCTTAGTGCCCCTCGGCGCATTGATGACCCATGTT TGGCCTCGATCGAGACGTCGACCACCGCCTATCGGGGTCGATGCTGCCCAAACGGTGTAGGGACAATTGT TGCGTACCTCGATAGTGGCAGCATAAGTGTAAGTCACTAAAGCAAGGAAGAAGAAAATAAAAGAAGATGT CAAGTAGCCCATGTTTGTGTACATATTTTTGTTTATTTTTGATATATAGTTACATATTATTGGGTAGTTT ATATAGGGGTGTGACAACTTTTGGCACCTTGCACATTTAGAATTCCACTATATAAGTTGTGGAAATTTTT ACCATAAAGAATTAAATGGATGGAATGATCATATGGAAAATTCCTCTAAAGGAGAAATTTACAAGCCACT TAAATTGGAGGAGGAATAGAGAAAGTCATCACATGAAGTGATATTAATGACAATATTATATAATTGTCTT TTAATTTGTTAGCAACAATAAAAAATTCATAGCTAATTCCTAATCTGCAAAAATGATTAATTCTTTATTT TCTAGAGATTCAAAGTTAATTAATTATAAAACTCTCCCAATGAATTTAGAAAACAGGGAATGTTTTTTGG TTCGTAGGCAAGAAAGTTTTTGGAATTAAACACAACAAAAATAATCCTCATATCCTATTTTTAACTAATA CTTTAATGTGTCTAGATTTTTATTAATATGTCTGCTTTATATTAAAATTTCATTTTTTTAAAAAATAATA GGAAAATTCTCAGACCGTCCATAAAGAAAAATCAAAATTAAATAGTTTCTTCTATTCAAAATTAAGATGA GTTGTCACCATACTTTTAGAATTTTTGATTTTTTTATGCAAGAATAAGACTTACGAGAACAAATGGTAAT CAAAATAAGATAGGAAAAAGGGTCAAATTTCCTTTTTTTTTCGCCTAACTTATTTTATTAACTAATTCAT ATTTAGTTGTGTCTAAAACAGGTTTAACTGATAAAGGTTAAATAAAAAAAAATGTTACTACTGTGTTGAT TTATCAATTTTGGGTTATTGAATTAACCGTCTTAGAATATTAGTTCAGCTTCCAATTTTAAAATTTTAGT TAATGATTAAATCAATAACCTAATAAAATTATATTAAAACTACTTTTTTTTATTTTTATTTCTATTTATA TAATAATGACTTTATTTAAACTTCAAATACAAAACCTATTTTCTTTTGCATTTTATAATCAATGTATTGA TGCTGATGTGTTTTGTGCACTTGTTAAAGTTGTGTTATTATATTATTTTAATATTAATATAATAAATGAA GCAGTGTTTTTGCTTGTAAATTTCTAGATTTCTATTTATGAAGTTCTGACTTAAACGTATCTAACGAATA ATTAAATAAAAAATCAAGTATTTAAGGATCGATAGTTGATTATCTAAATTATTGATCATTGATTTAGCAT GTTTATAAATAAAAAATCGATAAACTAAACCACAAAATCTAACCAGTAAACATTGAGACCATATCGAATA CACCTACAAGTAATTCATTAATAAGTTTCAAATTCAATATACAACAACAAAAAAAAAAGGCAAGAAAATG TTGAAGTAAAGTAAATAACGACACACGATTATTAAAGATATTATTATTTGTTCATCAATGATCAAAATAT ATAAGTTGAAGGGAGTGTGTATACATTACACAAATAAGATATTATTATCTCACCCAACATAAATAGTTTA ACAAACCTTTTAAGATACTTATTGTTTTGGTAATTTTTCATACCATATAATTTGTAGCAAAATATGCTTT ATATTGTGGCATTAATTATGGGCAAAAAACAACCTTATAATTTGTACTATCACTAGGGCAAGCAAATGTG CTAGTAGCATCATCTTGTGGGTAGCTATAGGCATCAGGGCATCTCTGTTTGAAAAATTTCGACCACTCAG TAGGACTACATGGACCTTGGGTGCAACAATATTGTTGTCCTCCAAACGTGGTACAAGGATTGTTACATCC TCCGGTTACTTTAAGTTGACTAGGGCATTCACCGTTTATATTGGCGGTGCATTGAATTGAGTGGCATTTT CCTCCACTGGAATTGGTTGTGGCGAAAGTCATCGGTATATTAAATCCATCGACTAAAGAAATGTCCCAGA AATCTAGGTTGTTAAACTGGTTCAAGGCGTATTCGGTCAAGGTGTTTGGCGGTTTACCCCACCCGGTACA CTGCAAGACTCCACCACAATCACCAGTCTCACAAGAACCTCTACCAGCACCGTCAAAGTTGCATTTAGTA CGACCCCATATACGTGCCATCTTAGTGCCCCTCAGTGCATTGATGGTCCAAGTCTGGCCTTGATTGAGAC GTTGAAAAATCTTTTTTTTTTCATGTTTCTTGTTTATTTTAAATTTTATATATTTGTTTGTTTGTTTTTT TCTCTGAAAGAACGTTTCTCTCTTCTTCTTTTTTTTATTATTTTATTTTATCATTATTTTTAGTATATTA TTATTTTATAGTTTTGTCTTCTTCTTTTCTTTTTTTTTTTCCAATTATTTTGTACTAATTTTGTTATTCT TTTATCGTTTTTTTTAATTGTGTTGTTATTATTTGTTTTGAAACATTTTAAGGGTTCGGGATTCCTATTA GTATCTTAGGAAGGTGTAAGGCACCCAAAACACTCCGTTAACTTACGGTTTTCCAATGATTAACTATTTG ATTATTTTTATTTTTAACCTTTGCAAATTTATTTGAACTTTTAACATGACTTACTTAGCCAAACTTAGCC AATTTTGAAATATTTATTGTTTATCTTGCCAGTTTACGAAACCATTTCAAAGTTAAGCTTACTCAAGGTT TTATCTAAACAAGCCTTACGAATTTGAAACAATTTATCATTTAGAAGTTAGTTTTAAAAATTTTTAGGTT TGATAAAATAACAGGCGTTCGATGAGGGTTTGGAGTTTCCTAATCCTAAGCTTACGGCATTGAAAACAAA ATAAGATAAGCAAATAGTAAAGAGAAGGATAGAGAGAGAGAGATTTGGGTCCAACTCCGGGTTCTGTTAT CCTTGACCGAATGTTGGACCCACCTGCCTTTGGGTTTTTTAGCCCATTTCTGTACCTGTCCTAATCTAAA CATACAAACATAATATAGAAAACAAAAAAACGATATTTTTGTGAAGTTAACTTTTTAATAAATGATAAAT ATAATAAATTTCAAAAATCAAATATATTTGGACGACTACTTAAATGTTTCAGTTTATTTTTTGAGCCAAA TTTTTATCAGTGTGAATTGAATTGATTCAAAATTTGAAAAAGAAAAATATTTTCCACCTCAATTTTTCTA TATATTTTGTGTAAAAATGATTTTTTTTTCCCGAAGTTCTTAAAGCAAACTTGACAATATCACAAAATAT TTAAATTTCATTACTTCAAAATGATCTAAATATTCCTGCTGAATATGATGTTAGTTTATAATGAAATTAG ACGAGAAATTCCTGAATATGACTGCCCACGTGAATTTTAGTTTTTCTTTGACAAAGAAAAACATGGCAAC TACTTATTAATTTTTTTAAATTGATCAAATATTTATTTTCAAAAATAATTAATTATTAAGAGTATAATTA AAAAATTATTATAATTTATACATTAATTTTACAAAATAACAAATATTACGAACTAACTATTTTATAATAA AAATGACATATAAAAAGATACGGAATAAAAATAATCACATATATCAATAATATTACATCGGAATAATGAT GAGGAAAATTTTCCACGAGTCACTATTCCACGTCCGCATTTAATTCTTCATGAATATTTTTTGGTCTTTT TGGGAACCTTCTCCTAACTTTACTCGTATCGAATTTAACTAAATCTAAATTCTTATTAAAAAATTCTATA TTAAAAATATAATATTTGTGATTTAAAATTGTGAACATCTAATTTTTTATCAAAATATAAAGAATCAAAC
Page | 44
GEN 3040
ACCAATTTTTTTAAAAAAAGGTTATATTTAAAATAAAATAAAATAATAATAATAAATTGTAAATTAATAA AACTAATAAAATCAATTATAGTTTATTAATAAAAAAAGAATTGATAAATAGAAGTTGGGAATTTAAGTTA TTTGTTAATTATCTAGTTTAAAAATAATTTAAGTTATTTGTTCACCTTATCCTAATTATTCCCAACTTTC TAACTTATTTTACTAAAAAGTTTCATTCCATTAATTTTGATTTTTTTTTCTTCTGACGTTCATTTGACTT TTTTTTTAATTTTTTTTTTAAATTTAATTTTCTATTATTATTATTTATTTATTTTATATCCTTATCTCAA CCCCAATTCCCTCAACAATATTTCAAACTAAATCCTAATCCAACTCTATTACTATCCATTTAATTAGCAT CAAACTCTCTCAGTTGAGAAGAAAACAGAGAATACAGACAAAGAAGAGACACAGACACACAGATACAGAG AGAAATATAGGGGAAAAAAACGAGTTATTTCTTTTGAGAATTAGGCTAGAGTCAGAAATTTGGAAATTTG ATCGTGATTCCGAATTTGTGGATACAGCTCGCTTTTTGTGGTATCAATTTTCGCAGCGAGCTAACTATAA ATTCTCGCGTGATTTAAATAATACTACTTCTATGAAAAATTGATTTTAATAGCAATCACATGTATTATAT TATTGTTACGTTATTACTATTATTATGTTTTTTTTAGATAGATTTTGCAATTTATGTTGCTTCATGATAT TGAGTTGTTGAATTTAAATAGATTAATATATATTTTCGTCTATTTTCACTTTAGAAGATTTTTGTTGAAT TAATTGTATACTTGTTAGATTAGGATATAAAAATAATAACTAATAAATAATTTTTTTTCAAAAAGAAAAA CTAATCTAAGTAGAATTGTAATTAAAAGGTAAATAAATTTCATTTAGAGATTAATATTATAAACTAATAA TAATAATAAAAATAATAATAATAATAATAACAGAAGTAAAAATTGAGAAATGATATAAAGAGAAAAGAGA TTTTAAAAAGAAAAAGAAAAAAAAAGGGGCATGAAAATATAATAATAAAAAAAACATAACAAAATTTTTT AACAAAAACAAAAAAAAAAGAACGTAAATTAAAAAAAAATACAAAAACGTAAAAAATAAAAAGGGAGAAG TAAGAAATAAAAAATAGAAATTTAAAATTAATAAAATAAAACGTTAAAAAAAAACAAATAATAACAACAC AATTAAAAAAAACGATAAAAGAATAACAAAATTAGTACAAAATAATTGGAAAAAAAAAAGAAAAGAAGAA GACAAAACTATAAAATAATAATATACTAAAAATAATGATAAAATAAAATAATAAAAAAAAGAAGAAGAGA GAAACGTTCTTTCAGAGAAAAAAAAAACAAACAAATATATAAAATTTAAAATAAACAAGAAACATGAAAA AAAAAAGATTTTTCAACGTCTCGATCAAGGCCAGACTTGGACCATCAATGCACTGAGGGGCACTAAGATG GCACGTATATGGGGTCGTACTAAATGCAACTTTGACGGTGCTGGTAGAGGTTCTTGTGAGACTGGTGATT GTGGTGGAGTCTTGCAGTGTACCGGGTGGGGTAAACCGCCAAACACCTTGGCCGAATACGCCTTGAACCA GTTTAACAACCTAGATTTCTGGGACATTTCTTTAGTCGATGGATTTAATCTGCAAATTAGAAATTCGGGT AAGGAATTCTGTTGACCGAAGGGAAGGTATTAGGCACCCCTCGAATCCCGTGGTTCTAGCACGGTCGCTT TATTAACTTTAATATGACCTGACTTAATTTTGGATATTATATTTTTTAAACTAATTATAAAATTGTTATT TATCCTCAAATTTATTTTAAACTTATTTCAAACTGGCTTATTTATTTTAAAGGGTGTTTATTTTTATTTT TATTTTTGTGTAATTTTAAAATTGAACATCCGTATATATTTTTTTTGCTTATATTTGTATGTTTTTAATT TTTAATGTTATAATAGGTAGAATATATATAGATATCTAATTTCTAAGAATTAGAGTTTGTGTAAAAAGGA CATATTTAGAGTTCAAGTTAATTTAGGATTACATATTTTAGATTTTATCGAGACTCAATACAGATACCAG ATATTAATTTCTACTGCAGGGTCTATTTGGGAGTGGCGCTTCTAACATGATTTTTTTCATATCCAAAGCT CGAATTCGAGATTTCTGATTAAGGTAAAGCATTCTCACTAGTGCACCACAACTCATGTTGGTCATATAAA ATTTTTTAATTTTTGTGATAATATTATTTTCTTCTAAAAAGTCTCACTCAATTAAGAATATTCTTAACAC TATATTTAGCTTCTTCTATTTTTCAATTATATATTAATATTTATTTGCACATATTAAATTCATAATTTAA ATTGCAATTGACCGCATGCAAATCAAAGTCTTCGTCAAATACGACGAGTCGTACACATGCCTTAAAATTT GAGGGGCTTATTTTTTTAGCAATAATATACTTATAAGTAATTTCAAAAGTAAGTATCATCTATTTCAATT TTTAGTTTAACAGTATAAGATTTAAATGATTTTTTTATTTAATTTCAATTATCATATCAAGTCAAAAAAT AAGATAAATAAATGGAAAATTGAAAGTACTATTTATTGAATAAAAATAAACTATTTCATGTGAAAGAAAA AAAATAACTTTCCATCACTTTTTAAGAAATATATTAGTAGCAACTTTGTATTCTAAAAAAAAATATAGAA GAATGGACTTTCTTTAAATTTTAAATAGTACTTATTTGAATTATAAAATAATCTCAATTAATGTTCTATT TTTTTTCCAAGAGATATATTATGGTTTAAAAATTAATCCATGATGTGGAAAAATGATCAGGAAATTTTCC CACAAACCACTATATAACTTCTTCTTTTCTGTACTTTTGAATTCGATTAAATTAAAAATTTTGTTAAAAA ATTCAAATTGAAGATATAACTTTCAACTTTAATGAAAATTTTAGATCAACATTTAAATTTGAGATTACTG GAAAAAAAATTGGCATGTATTACTTATGTAATAATAGTTATTAACTATTTGTTTTAGAATTATCAAAATT CCAGTATAATTAGGTGATTCTTTTTTGGGACATTGTGGGGTTTCAACTTTCAATTTACAAGACTAGCAAG ACTTGCTGTTGATAGGTTAGAAAAGTCTTTGTAAGAAATGAATTGTATTATTTTTCAAAAAAATTATAAT ATAAAAGAAGATATAAATACATGTGTAATGATATATTATCTAATTAAAATAATAAGTATTTTAAAGTTAA AGTATATTACTAAATATAAAAAAGATATTTATCATTTATTCAAAACTGATTAAAGAAAATCACATAAATT AAAATGAAAGAAATACAGCCTTATTTTCTCAAATTAGTGGAAATAGTTAAAATTTGAATTTGAAATAATG ACATTGCAAACCCGTATTGTATATATAATATATCAAAAAGCTATATACCTATAGGGAAATATAATTAATA TATAAATTTTTCTCGTCAAGTAAAAAAGTGTTAATACATATCTTAATATATAGATATTCATTCAAATTCA AATGTCTTAATTTTAAGGAAAGTATTAGAAACAATTAAATGCTTACAACTAAACGAAACAAAAAATTCAA TCTGTCGGTGGCATTTACTGACACTAAAAGGGAATAATTAGAAATGTTCATTTTTTAAAAAATAAAATAA AAATAGAAATCTCCTATAACCAAAAACGACATTTTCTATTAATTTACCCTTTATATATTTTTTATCACTT TTAAAACACAATCCTAATTTTTAGGGAAAAAATAAATTAATTCTTCATATCTTGATTTTTAAATTGACTA GGATTTTAAAATGAACTTTGTCTTTAATAATGTTGATAATTAATATGAGACAAAGTCGGAGAATACTTAA
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TGGCTTTAGTCATGTTGCTCAGAAAGAAATGTTTAATTAGTTATATACTACGGAAAAGGGTTAAAATATT CTTAAACTATCAGAAATAGATTTACCCTTAAATTATATTTTGATTTAAAACTATTTTTCTATCAAATTAT TGGGTCAAAAATACCCTTCTTATTTTTTTTACCCTTTTCCGTATATATTTTTAAAAAAGACATTTTGATG AAAATTTTTAGAAATTGGAGCAAAAAGCATTAGATGATCATAATAATACATATTGCAAATATTTGACTGC AAAGCTAAGTCTTTGTCAATAATAAATATGAAGCTATTGCAATTTGTGGTCCTCATGCATTGGAAATAGT ATGCATAGCCTTTTAATTAAGATGTACATTGAGTTACATAGCTGGAAAGTCGTCGTTTATTAAGAGTGTT TTATTTATTAATGTGAATTTTTTAAATACAAATTTATATTTAATCGAGATTTAATATGAATAATTAAACA CCAAAATTTAAATTTAAGAAGGTACAATAATTTACCCCTCCTTTTGTTTTTAACTAGCTAGATCGAATTG TATATATAGGTAATCATAATATTCAAATTGTGCAATATTTGACTGTGTATAGCTAAGTGGTCAATTTATA GTTGGTCGTCATCACTAGAACTTTATAGCCTTTTAACTCTTCTTTGAAAAATTAAATTGATTAGAATACT TTAAAGAAATTAATTTAATTTCATGTGAAAAAGTAATTTTAAGCCACTTAATTAATTTCATGTACTTAAT AATGGGAACGAGGAATATAAAATTATATTGGGTGAAGCTTCCACAAAGAGAATCCTTAATATTATAATAT TATATAATACTTTTTGTTCTGAATTAAATCTCGATGATTTTTAAAATAAAATCTAGCTCGCTTAATTAAG AGTGTTCATAATGTTAATCGTAACTTCTCTTACTTTTCAATTATATGGCAAATGTGAAGTTTTGCACAAC TAGAAAGCAATCTCCTTCTCGAATTAAGTTTCATGTATGCATGACTTACCACTTTATTCCTCGAATAATT TTACATAGCTCATCACTAAAATCGTCGATTCATGTGTTGAAATGGATATTAATTATCTGTCACGTATAGT TTTCAAATATTTATGATCATCGAAGTCCATATATAATGCAAGCTCCTATTATTATTAATTTTTGTCTAGC TTTTCATAGAGGGTGAAAGACGGATCAGACTCAATACCATGCATCTTCATACTCATCTCGTCAAAAGCTA TTAACTTAAACCTTGAGGGAAACTTAAAAGTGACCAAATCATCATAAAGATATTCTTTATTTTCCTTTTA TTCATCACAAACTAAGGAACTACATAGTACTTCCATATTATCACTATATATATGATGAATAAAAACATCA TATTTTTCCTTGACTTAGTAATTAATCCTCCTAGCAAGGAGAATAACATAGCCTTATTCAAATTATTAAT ACAAAAGGATATATAGATAGGCAGATAGATATTTAAGGACAGAATACAACCCTATAATTAGTACCAGCAG GGCAAGTGAAAGTACTAGTTTGATCATCCTTAGGGTAACTATATGCATCAGGACACCTCTCCTTAAAAAA TCTCGAAAATTTAGTCGGACCACAGTTACCAGAGTTACAACAATATTGATCAGTCTTAAAAACAGTACAA GGGTTGTTACATCCTCCGGGAGCTTTCAATTCATTAGGACATTGTTGATTAATTTCCGCTTTACACGTTA TGCCACGGGTGCACCCATTGGAAGTTGGACTAAATTCCATAGGAACATTAAATCCATCGACAAGAGAAAT ATCGAAAAAATCTTTATTTGCAAATTGGTTTAGGGCATATTCAGCTAATGTATTTGGTGGTACACCAAAA GATTTACATACAAGAAGACCATTACAATCTCCAGTTTGGCATTTTCCCTTACCGGATGCATCGAAATTGC ATCCGGTACGGCCCCAAACTCGAGCTTGTTTAGTCCCCGGAGGGGCGTTTATCGTCCACGTTTGGCCACG ATCGAGGCGTTTGCCGCCTCCGGCTGGGACTCCGGCAGCCCAAACGGTGTAGGGACAATTGTTACGAACT AAAATTGTGGCTGCATTGGCATAGGTGAAAAAAAGGGAAAAGAGAATAAAAATTGAAGTTTTCATATTTT TATTTTTTGTGTGACTACTAATAAATTGGGATGAGTGATTTTTTATGAGGGAGTAATAATAATTAGAAGG GTATTTATAGGGTAAAAATGGGGCACCAATATAGTTGGTAAGTAACTAATTTGTTTGAAATTTTAGTTTG TGATAATGTAATGTAATGTTTTGAGTAGACTAGTACATTATTCTATCGAGGGTGCAAGGTGTGGCTACTA GAAGTTGTAGAAAATGTAGCTAGGGTCTATTATTCAAGCTTGGATTTTATTTTTTACTTTTGAATAATAT GTATATATATATATATTTATTTTTTTTACGTATCAGAACGAAGTTCGATTAAATTTAGATACGCGCTTTA AAGTTGTATATTGAGTATAAAACGTTTCCTAACAAAAATAACTATGTACTTAAGAGACTCGAACTCGAAA CATCTGATTAAGAATTAATGAAATAGAATACTTATCACTTCACGGCCAAAAGCGAAAAAGAAAAATCAAA GTCAAGAACAATGAACTTAGAATTTGGATGGTACATAAATTCAAACTTTTTTAGATAATCATGCAAGTTT AAGGACCTCTATTTAGCAACAATCGAAAGGCTATGCACACATGAACATTATATTGCTTTGTGATTTACAT AAGTGACTTTTTTCAGTATCCACATTTGATATTCATATTGAATTTTAACACTCTATAAATAATAAAAATC GTGATTGAATTCGATCGAAATCTTAAATTAAAAATTTGAATCAAAATTACTGAATTCTTATTCGTCAATA TTTAATGATCTGTATTAGTGCCCCTCGAATCAATAAAGTTTAAAAGATGGTGACTTGTTAAGTGTACAAA AAAATCAAAAGAAAAATAGAGACTTGAAAGTAAGAAATATCAAAGTTTACTAAATATGAAAGAAAATTGT TAACTACCTTGTACAAAATTAGGTGGTGGAAAATTCGTCTTCAATCATATAAAGGCATTTTTATAAAAGT TATGTCATAGACAAATGACTGGCCAAAAAGTTATATATTTGCTAAAATAAAAGGTTTAAAATTAAATAAT TTTTAAATATAAAATATATTAATCTTTATGAAGTAGATTAAAAAGAGATGTATTTATATTAAAAAGAATA AAGACTTTTAAAAAAATAATTATAAATAAGAACAAAACTCGAATAATATAGGAAATTTGTAGAAAGGATA ATGTCTCCAAATAAAATATGGGACGACTCAAGCAAACAGTGCTACAATTCAAACGCAATTAACAAGTGAA ATAAAAAATTTGTCAGAAATTTCAAAATCAACAAACGATTTATGAGGTTCAAAAATTTATAAGAGTTTCA TCTTTCTTCTGCATTCTAATATCTCATTTCCAATAATACGTTTTAATACACTTTGTCTCAAATACAAATA GTAAATGAGTCAATATATAACGTAAAATAACGGCGATATACGATAAATATGGGAAATCTAAGCATGTGTG AACATAACACATAATAAGTCATCCATTATTTACTGATTTATGGTAAATTAAGTAAGGAAGAACGTGACAT TTTATTATTATTTAATGAAAGAAAATACTTTGAGAATATATTAAACATGACTAAGAGAATTCAATATTTT ATACATTTTAATTTATGGCACATTAATATAGTTTTTCCACTTCTGTAAAAAATATTTTAACTTAAATTAC ACTTTATCTAGTTTCTAAAGACGAAAATATTAATTTTCAAACAATAATTAGTCTAAACTGATTAATTGAG CTAAAAAGGTAAAAATTGATGAGATTGTATACCAAGAAGATTATTATAAAGTAGTTTACTATGAGAACAT
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ATTATTGCAAATGAGTGTATTGATACTTTAACCTAAATTAATGTGCCAAGCTAATGTATTTTTTATCAAT AGTTTACTGGACTGATATAAAAAATAAAGGGATTTTTCCCCTCGAAATTTTGATCAAAATTTATTATAAT CGTATTTTATTTATGTTATTTTATGTTTTAGTTTTTTCTATTCTTTTAATTTTAATAGCTTTTTATTTTA AAAAATTAATTTATTTTAAAATAAAAAATATATTTAATATTTAAAAAAAATTAATAAAATTTTCCGACCA TAAATAAAATATCATATGCAACTTATAAATTAAATGAAAAAAAAAACTTATTTAAATGTAAGGAATATTT TTAAAATTTAGCTAAAAAGTTTCCCAAGTTACGAGGGAGGGAATATGGATGTTCATATTTTGGATAAAAT TGATTCGGAAAGCTCAATCAAATTGATTAAACAAAATAATTTGTTTTTTATTTAATTTTACATTTTTTAA ATTAGATAATAGTTGGCTTTCCTTAAAAAGCTCAAAAAAAAATTAATCAAATATATATTATGAACAATTT TAAATATTGTATATATGCTTTCGTCAAGATTTATATATATAAAAATTGTTCAACACAAGAACATCTATTT TATGTATCAATTTTATTTATGTGAGTTTTTTACGAGATTAGGAATGTCATTGCCACTTCCTAGTCAGGGA CAAAGTGGTGAATTTCAACGCTTTGTTCACATTAAATTTAGTCTTTGAAAGTTTTGTAACGTCATCCGAT CTAATTATTTTTTTGTTTGAATAACTTGTTAATTTTTTCTTCCCTTTTATATGAATCAGTTCTTTTATGT TTTATATATGATTAATAACTGAATAATCGAATAAAATCACATTGAAATCGATAACAATCAAGCTGATAAA TGTATATTATATTAGGTTTGATTTGATTTTGATTTTGATCAATAACTAACCCTAATCGACTTGTGAACAC TCATAGTTGATGATAGTAATGGTTGTAGTTGAGTTGAGGACGGTAGTTATGGACGTTAGATGATGGTGAT AATTATGACTGAAAATAGATGAAACAGTGATGATTTGTTATCTCTATAAAATTCTTAAATAATAAACATA AGACTTCCATATAAATTTCAATCATTCCGACTCATTTAGACCCATTAAGTACGGTAAAGATCAACACGTG AGTAATTAAAAACTCATTCACAGTATTTCAAACTTGCATTTGTTAACATTAAACATTTTACAAAACACGT GAATTATTAACCATGCAAAAGAATTCCAAATTTGCAGAATTCGACCATCCTTCTGTGACTGAATTCAATA AAGTCATATCGATAACTTTTAAATCCGTTACTAACAAAAACTATTTGTATGCATATATACAAAAAAAAAT TGGCTTATTTTTTTAGTCTAGTTGAAAAATATATTATCTCGAGATAAATTATTACCCTAAAGAGAGACGG AGCTAGAAATCGAGTAATATAAAAAAGAGGTAAATGAAACGGCGTTAAAAAAAAAAAGTCCTTCCTCAAC CCCACAGCTTGAGTGTGTTTCTTCTATAACACACTACTTTTGTATATATATTTTCAAATCCAACAAATCC GTCAGTTACTCTTCAGAATCTTCTTCAGTTTCCCGCTACTCTCTCTCTCTACAAATTAATATCTTTAAAC CCTAACTTCACTCAAAAACGGTCAGTTACATTCAATTATACTCTCTTTCTCTCTCTACAATTTAGGTACA TTTTTTTTCTGATTTTTATTTAGATTTTACGAATTCGAAGCAATTTTAGCTGTTTTGTTCATCATTGTAT GTTTTCAAGCTGTTTTATTTTGGATTTTGCGGTTTTTTGTTCGTTCGTTAGGTGTGAATCTGGTTGTTGT GAATTTTTGATCCTGTTCGATTGATTGATGGAATGTTAGTGTAAAGTTTTGATTTTTTTTAGTTAAGAGA TGTAAAAAAGGGGATTTTTTTGTGTGTTTTATGCACCTGGGTGCATCTATGGAAAGGAATAGATATATGA AAATTGTATTGGAATGAGTGAGTTTGAGTGGTTAGGAATGATTTATATAGCGGATCTGTACTGGTTTGGG GTTCAGGTGTACTTCACTGAAACAGTTCATTTAATTTGACTACTCTGATTAGCTATATCTGTATCTAGGT TTTAGGTGGATTTTTATGTGTTTTATAAATTTGTTTTTGTATCCATTCCCTGTTTATTTCGAAGCTTTTC AAGTGTTTATCTGCATTTCGTGGCATCTTGTTCATTATTGTATGTTTTTAAGCTTTTTTGGGGGGAATAT ATTTTTTTTTGCTTATTTGTGTTTTTTATTAAGGGGTTAATTCTCTTCGTTTGGAAAATCTCGTGATGTT TGATTGCTGAAAAAAATGCTAGTTTAAAGTTTTGATCTTTGTGTTGAATTTTGAGTTTTTTTTGCTTAAA ATTTGGACTTTTGTGTGTTCTAAGGTTCTTGAGGAGGTACTTTAGGGATATTCATGAAATTGAAATTATG AAGTTGATTTGTTTGGCTACTATGCAAAAATGATGTTCGTATTGCATAAATTGATTTATATTGGGATTGA GCTTGAGTTGATACAAATGATTTTGATAGAAGATCTTTACTAGTTTGGGGTCAAGGTGTGGTAATTGATT GATTAGTTCCTGAGGAGCACACACATCTTTATTGTCTTTCTCTTGCAGAGCTGTGAGATATTTGATCGTA TTACTGGATTGTTGGGAATTCTTTCCATACTTTTGTTTGGGTGCCTTGAACATTTAGGATTTATGACATA TGTTTGTTTTGTTGAAAAAGATTCTATTTTCTTACTTGGTGTAACTAATAGCTCTCGCGACTTCCATGTC TAGATAAAAGTAGAGGGTTATAAGTTGACAGCTAGTATAAACGTAGTCTAGCATTATACTGATTGTCGGG CACAACATGTTTACTGATGCGAATAGAGCAAAATGTGGTTTTAATTTCTATAACTGTTCTTGTTTTTCTT CATTAGCTTGTGCATTTGAGCATTTGTTCTTACTTCTTCTTTGTCGTAATTGTTTTGCAGTTTAAGGATA TTCTTTGGCTGCAAGACATGGTACAAGTTAAAGAGGAAGCTCCAACTCTGGACATTGGTGGATTCATTTC TTGTTCATCTCTCTCAGATAGCAGTTATGAGGCTAGCACTCCGAGATGCTCCTCCGAGCCTGGGTATGCT GAAACCTTTGATTTTTTTCTGTCTTTGAACAGTTGGGCCTCAAATAAGGTCTTTCAAACAGATACGCTGA TTGATGCAATTTTAGTTTTCACTTCAATTGTTTAATCCTATTTCAGTTCCAGTTGTCGAAGGAGCTCAGG TCCAACTAAGCGTTCTTCTCAGGCAGGCTGGACAGAAGAAGAGGTAAAAGGCTAGTCGCTAGAGCTTTTG TTGTACCTATATGTTGTTTGATTTCTAGTGCATTAATTCTCCATTGAAGTATCCTGCATACATGTATGAT GGTTTTTATAACCTTGTGATGAGAGTGTGTCTCCGTGATAGTTAGGTACTTATAGAAGTATCTGTATGAG TTGTCATCTCCTTTTTCCTTTTGGGTTCTGATGCACGTGGATGCTTAGGATGTATAATTGAGCTAAGGAA AGGATAAAGATAGATTTTGTTACTCATAATATCAGTTTAATTTACTCATTTTTACTATTTCCATAGGCTC ATTTTCTTTGATCTAGAAATTTCTGACATCTTTATTTCTTTCACAGGACAATCTGTTGACTGAAGTGGTG GAACGGTTCAAAGGAAGAAACTGGAAAAGAATTGGTAAAACTATTTACTCTGTCATGCTCTATACTTTTC AGCTAGGTTAACCACAAAGACCTTCACTTTTTGCTCCAAGTCACTTTATGACTTGGTTTCCATATTTTGA ATACCTGACTATTTCATGGAGTAACTTATACTGTTGTATCTATGAGCAAAATTTGTCTGCTGAACATTTT
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GTGTGGTTTTATTGTGTTCTCTTTTGTTCTATAGCTGAGTGCTTGAATGGAAGGACTGATGTGCAGTGCT TGCATCGCTGGCAGAAGGTTCTGAATCCTGAGCTTGTAAAGGGTCCTTGGACAAAGGAGGTATTAAACAG TTCTACATCCATTGGTTTTCTACGAGTTGTATTCCAATTATCAATACTTTGATTATTAAAGACAACTGTT TTGTTCTATTTGGAATTTCAGGAGGATGACCTGATTATTGAGTTAGTTGAGAAACATGGCTGTAAGAAGT GGTCCTCTATTGCAAATTCTTTGCCTGGTCGCATTGGCAAGCAGTGTCGGGAAAGGTCAGTTTCTAACTC CGCCTAAATCTGTTGCTAGGCCACAGCTTCTATTTATACCTCCGACTAATTTACATGATCATAAACCCAG AATTTTTCAAAAGGCTTCCTGGAACATCTGTAGTTATGTTGCTTACAGAGTTATTCCTTGCCATCAGAAC TCATTTTGTCTCACTTTTCTCTCGTGTTTTGCAGGAATTGTATCTTTTTGTCTTATTTTCACTTAGTCCT ACTGGATTTTTTGTCCTGTTGAGGCCCTGCTCGTTTGTAGGAGCATTGTATTTCTTGTTATCTATTGCTT GATATATTGTGTGCGTCTTTTCAAAGCAATTAATCAATCAACTACTGCCCCAATTCGTAGTAGGGGTTGG CTATATGAAAAATCCATAATTTGGGGCACAACTCTTCTTTATAAGGATTCAAACTGGCTATGTTTTGCAC ATAGGCAGATTTCTCTTTTGTAAGTGAATAAGAAAAAGCATGAAAACTGGGAGCCTGCTAAATATTTGGT TACTGCAATTTAATGGTATATTATTCGTTGGATTTTGCAGTTAAAGCTTAAAAGGAGATCCACATTTCTA TGTTCTGTTCCTTTTGAATAAGAAAATGACATTGGTTGATTAATTGCAAGTTATCATAGTTGTTTCAGTC TGCTATGTTCACTAACGTTGTGAGATCGTCCATGAATGTCACAGGTGGCATAATCATCTTGACCCAACAA TAACAAAAGATGCCTGGACTGAACAGGAAGAAATGGTCCTATGCCACTATCACCAATTATACGGAAATAA GTGGGCTAAAATTGCGAAGTTTCTTCCTGGAAGGTATTCTTATAAAACTATTCTTCTATTATGTATATCA GTGTCTGATTTTGAGATTCCTTGGGCATTCGTAAAATGTGGATCAAGTAATTTGTTATATCTATCACCCT CTCATACCAATGGCAGATCGTTTGGAGTGCATGATTCTGAAAAGTCAGTATTAGTGTGGTATAACTTGTA TGCCAAATCAAACACAACTTTTTGATTTTTAGGTTTCCTTGGTACATTGCTTACAGAGTGTATCAAAATC TTGGATACTACTCGAGTTTGTTCTAGGGAGATAAGTAGATAATTTTTATAATGAAGGCAGTGAAGGCATA TGCAGCTTTCTCTTTACTTTTACTTCTTTTTATCTTATTCTCCAGTTTCACTTCCATCCAGACTTCTAAA TCATTTTCTCCCAATATGGTTTCTTCTTTTACTTCTTGAGGTTCAAAACTATTCATGTCTGGAACTCTCT TAATATTTTATTTTGTCAATTTGACATGGAAAAAAAGTTGAAAGAATCATTTTGGGCCTAACTCCACCCC AATATCTAGTTCAAGAGGGAGGAATGCCCAAGACTATGTATGTAGATCAGCCATCTCTTTTTCATTCTAT AGAGGGCTCTTAAACACTCTCGTCATGCCCAGGACTGAACATCAGGAGTATGGGCAATTCAAATAGGGGC CCAACATCGGGAAACAAGAATTGGGATGAGTTCTGGTATCACCAGGTAAAGAAATGGATCTTGGGACTAA CTCAACCCCAAAAGCTAACTCATGAGTGGAGGATTTCCAAGGCCATATACGGAGACCACCTATCCCTCTT TCACCCTATGTGGAACTCTTCAACAATTTTAAACTTGTAGTAATAAGCTAAGTTTTTTTTTTTTGGTCAG ATTGACCCTCGGCAAGCAAGTTAGGAGAACCAAATGGGGACAAAGTTAGTAATAGTATTATAACTTCTTT CTGCAGAAGCCTGGATATTCTAGGTATCGTAAGTGTACTTTTAAGGACTCAATCGTGCACTCTGTTTAAT TCAGAGGATTGTCGTTCTTTTGTGCTAATAGCTGCAGGTACATGTGTCAATTTGTATCTATTAAGTCCAG TTTCTAATTTATCATGATTACTCTAAAATGATTTTTTTGCCTACGCTTGATGCGTTCTGCTTGAATGATT TTTGCTCAAGTATCATATAGGTACATATCTTTGATTAGTTCTCCTTTCTGATCTATTTTTCCTTTTGGCA CTCTCTCAGGACTGATAATGCAATTAAAAATCATTGGAATTCCACTCTGAAGAAAAGATTGAATTTGAAT TTACTAAGTAGGTTAGTGCCGGATATCAAAAGTGAGGGATCTCCGGACTTCTCAACTCACAAGAAAAATA TGGAAATCAAGAAGCATCCAACACAGGCTCATAATGCAGAAACAGTCTTTTTGAGCGAGCAAACAGGAGT AGGTAATGCTGCTGATGCTTGCCCAACTGATTTGAGGATTGGATCTGCTAATTCTCCTCAAAATGGTTTG CATAAGGGTACTTCTTCATTGGGAACCTGTAAATCATCAGAACAGAAGGAGAGGGATCTGATAAAGCAAT TTGGTGGAATACAATTTGTCAAGGCAGAAATTCTTCCAATAGGTGAGACAGATAAACAATGCCAATCCAC TTTAAGTCTCACTAAAATATCATATCCTCTGTTGGACTCTTCTTTAGATGCTCGTTTGGATCCATCACAC AACACCGGATGGAGTAGTTCTCAAGTTGAGGCTGTTCGTCCTACTTCTTTTGGGAGTATGTATGAATCTC CCAAAAGGTCTAGGTACGATGCTGCCAATGATCCTGACCATGATTTCTTGAGTTTGTCATTGGCTGCCTT CACCGAGGTTTCCTACGGTAACAAGAAAAACAGAACATATGATACACAATATTCTTTGGCTCTCAATCAG CCTGGCCGCTTGTGTTATGAACCACCAAAAGTAAAAGACCTGATGATTTCTTCAATGGATGAAAAACTTA GTAAAGACAACTTTATCGGGCAACATCATGGCCATCCACTTTGCTCTACACCTCCTAGTCTTAAGTTAAC AGTCTCTGCTAATGGTAGCAGTCCAGAATCTGTGTTAAGGAACTCCGCAATGAGCTACACAAAAACTCCT TCAATCATAAGGAAGAAGAATTC
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Figure 10. The cladogram tree of the genetic relationship of the cloned sequence and five others using ClustalW
Figure 11. The phylogram tree of the genetic relationship of the cloned sequence and five others using ClustalW Q3. Have both the cDNA-derived sequence and the genomic sequence of this gene been determined? Yes. Both the cDNA-derived sequence as well as the genomic sequence of the Nicotiana tabacum osmotin gene were determined. The entry number of the genomic sequence in NCBI os X95308 and the cDNA is M29279. Q4. Are there likely to be any post-translation modifications of this gene product? What are they/what is their purpose? There are post-translation modifications of gene product and the they are the protein motifs at specific sequence found in the amino acid. For this sequence, the sequence motifs were determined by using Patmatmotif program (http://inntemp.weizmann.ac.il/emboss/output/220946/outfile). The protein motifs found were EGF_1, THIOLASE_3 and 2FE2S_FER_1.
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######################################## # Program: patmatmotifs # Rundate: Thu 19 Aug 2010 20:00:04 # Commandline: patmatmotifs # -auto # -sequence /var/www/html/emboss/output/220946/.sequence # -nofull # -prune # -outfile outfile # -rformat2 dbmotif # Report_format: dbmotif # Report_file: outfile ######################################## #======================================= # # Sequence: from: 1 to: 1304 # HitCount: 6 # # Full: No # Prune: Yes # Data_file: /usr/local/share/EMBOSS/data/PROSITE/prosite.lines # #======================================= Length = 12 Start = position 1271 of sequence End = position 1282 of sequence Motif = EGF_1 GTCGACTCTAGAGGTCCCCGGT | | 1271 1282 Length = 14 Start = position 1053 of sequence End = position 1066 of sequence Motif = THIOLASE_3 TTCTGAAGAACTACATGGGTCCGG | | 1053 1066 Length = 14 Start = position 1016 of sequence End = position 1029 of sequence Motif = THIOLASE_3 GGAGTAAATACAATATAAATTCTT | | 1016 1029 Length = 14 Start = position 830 of sequence
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End = position 843 of sequence Motif = THIOLASE_3 CAGAGAAGAACAACACAGAAGATT | | 830 843 Length = 14 Start = position 85 of sequence End = position 98 of sequence Motif = THIOLASE_3 AGGAAGAAAACAAAAGAAGATCTC | | 85 98 Length = 9 Start = position 1244 of sequence End = position 1252 of sequence Motif = 2FE2S_FER_1 ACTACCAGAACATCAACTC | | 1244 1252 #--------------------------------------#---------------------------------------
Q5. How many amino acids are present in the mature protein? By using the Expasy-Translate program, there were 246 amino acids were found in the sequence.
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Q6. What is the molecular weight and the isoelectric point of this molecule? The molecular weight and the isoelectric point of the molecule were obtained from ExPASy Proteomics Server by using Compute pI/Mw. The isoelectric point obtained is 5.09 and the molecular weight is 109296.44.
Compute pI/Mw Theoretical pI/Mw (average) for the user-entered sequence: 10 20 30 40 50 60 CGCGCGAGCG CCAACGGTGT ACGGGCAGTT GTTTCGGACC TCGATAGTTG CAGCATAAGT 70 80 90 100 110 120 ACAAGTCACA AAGGCAAGGA GGAAGAAAAC AAAAGAAGAT CTCAAGTATT CCATGTTTGT 130 140 150 160 170 180 GGACATTTTC TTTTTAACAA GTTGCGTTGT TGGATATAGT GACAAATTAT TTGGTGGTTT 190 200 210 220 230 240 ATATAGGAGT ATGGCGGCTT TTCGCACATA GACATTAATC ATATATATAT TTATTAACAT 250 260 270 280 290 300 TTGACTAGTA AATGGATAAT TCTCAAGGAT GCTTTAGTAA AGGTGGAGGC GGCTAAAAGT 310 320 330 340 350 360 ATAAGAGCCG CCTAATAAAC TAATAATTTT ATGAATATAG AAGTCAATTT ATTTGGTGAT 370 380 390 400 410 420 GATGCTGACC TATAAGCTCT GTAACGTGAC ACTTGTAACA ATATCTCTTT TAGTCAATTT 430 440 450 460 470 480 CTTGCTTTCT TTTATCTATA CCATTTTTTT AATTCACAAG AAATTGGCAG ATAGCCATCA
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490 500 510 520 530 540 TTTTGAAGCT ATTCAATATT AGTTGCTTTT TAACATGAAA ATAAACTTAA ACAAAAATAT 550 560 570 580 590 600 TTTTTCGCAA AAATAAGAAA AATATATAAA ATTTTTATTA GAGTTTAAAT ATTTATCAGT 610 620 630 640 650 660 TTGAACTTCA TATTTCATAC TCCCGAAAAT TTTTATGGAG TTTGCACAGA TCATAAATTT 670 680 690 700 710 720 TTCATGTAGT TTTAATACTA AATCTTGGAG TTTCACTTTT AATATTGATG TTATATTGTC 730 740 750 760 770 780 CCCTACTTAT TTTTTCTTCC TTCGTTTTCT TTTTGAGAAC TATTTCATCT ATTTATATAT 790 800 810 820 830 840 AGCAGCTCAT TAAGTAGATG GCGTTATTCT GGATGTGGAA TAAACAGAGA AGAACAACAC 850 860 870 880 890 900 AGAAGATTAA TGCCAATCGG GTAGACTTGA TCAACAATTT AACAAATAAG CTGATTGTAG 910 920 930 940 950 960 ATAACATAAA CTCATAAAGT TACGCCAATT CAATAGAGTT TGTTGAACAA TTGAACTAGC 970 980 990 1000 1010 1020 ATAAAATCAT GCCAACTGGA TAGAGTTTGT GAACACTTAA CAATATAGGT GGAGTAAATA 1030 1040 1050 1060 1070 1080 CAATATAAAT TCTTCCGATA GAAAAATTTC TGAAGAACTA CATGGGTCCG GAGATCATTT 1090 1100 1110 1120 1130 1140 TTTAAATATA TTATAAAATG GAGACAATAT TTAAAGACCA GTCCGTTATA GGCCGGCCAT 1150 1160 1170 1180 1190 1200 TAATTCTTGT CCATCAAATT TGCATCTGCT GTTGGTTTTC TGCTAGATCT ACTTAAAATT 1210 1220 1230 1240 1250 1260 TATAAAACAA TCAGTTTCGA AACAAAGTCT AGTGTAGTAC TACCAGAACA TCAACTCATA 1270 1280 1290 1300 TAAAAGTCGA CTCTAGAGGT CCCCGGTACC GAGCTCGAAT TCGA Theoretical pI/Mw: 5.09 / 109296.44
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GEN 3040
Q7. On an annotated printout of the promoter sequence highlight and list the features common to the regulation of eukaryotic genes. In addition, using web-based promoter analyses too and your knowledge of gene transcription/regulatory machinery and the references listed, locate and record the position on a printout of the above file any sequence motifs in your promoter sequence which may be important in regulating the expression of this gene? A promoter is a part of the DNA that is essential in the transcription of a gene. Generally, promoters are located on the same strand close to the gene they regulate. The RNA polymerase must first attach to the DNA sequences close to the gene for transcription to occur. The promoters contain specific DNA sequences and response elements which provide a binding site for the RNA polymerase to attach as well as for the attachment of the transcription factor. The location of the promoters is usually adjacent to the gene which is near to the transcriptional start site. If the gene sequence encoded for the promoter goes wrong, the amino acid could not be produced. As a result, the production of the protein will be affected and the wrong promoter sequence will regulate the expression of the gene. The sequence motif or the DNA-binding proteins in the promoter region regulates transcription, polymerases, histones as well as nucleases. They are also capable of incorporate the domains that aid the binding process to the nucleic acid such as the leucine zipper, zinc finger, and helix-turn-helix. The DNA-binding proteins involve the chemical modifications such as methylation, acetylation and phophorylation that could make the DNA more or less accessible to transcription factor. As a result, it affects the rate of transcription process. The DNA-binding protein also regulates the expression of the osmotin gene. The TATA box is essential for the initiation of transcription and bind to the specific region. The sequence of the TATA binding protein can be obtained by using fuzznuc (http://inntemp.weizmann.ac.il/emboss/output/470819/).
# Program: fuzznuc # Rundate: Thu 19 Aug 2010 20:29:38 # Commandline: fuzznuc # -auto # -sequence /var/www/html/emboss/output/470819/.sequence # -pattern TATAAA # -outfile outfile # Report_format: seqtable # Report_file: outfile ########################################
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GEN 3040
#======================================= # # Sequence: from: 1 to: 1304 # HitCount: 5 # # Pattern_name Mismatch Pattern # pattern 0 TATAAA # # Complement: No # #======================================= Start 565 1024 1092 1201 1259 End 570 1029 1097 1206 1264 Strand + + + + + Pattern_name Mismatch Sequence pattern . TATAAA pattern . TATAAA pattern . TATAAA pattern . TATAAA pattern . TATAAA
#--------------------------------------#--------------------------------------#--------------------------------------# Total_sequences: 1 # Total_length: 1304 # Reported_sequences: 1 # Reported_hitcount: 5 #---------------------------------------
Q8. Why is EDTA included in the GTE buffer? EDTA is responsible in the removal of the magnesium ions. The ions must be removed as it is essential for the preservation of the cell envelope structure. EDTA in the GTE buffer works by protecting the DNA from the enzymatic degradation in the chelation process of free divalent cations and also capable in causing the bacterial to burst (Brown, 2006). Q9. A manual sequencing autoradiogram of the Sanger dideoxy reactions often has a region where there are terminated fragments in all four lanes with a fainter sequence continuing past this point. Suggest a reason why this may occur. What might you expect to see in this region of you sequenced this DNA fragment using dye labeled dideoxy nucleotide terminators? There are terminated fragments in all four lanes with a fainter sequence continuing past this point as it might be caused by falling of the template by the DNA polymerase. The phenomenon can be explained by the secondary structure of DNA template, the hairpin loop that leads to the falling of DNA polymerase. As the replication happens, the sequencing fragments in the manual
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sequencing which are visualized by radioactivity attached into the sequence fragments and causes the premature fragments termination. As they have no labeled terminator, the premature terminated fragments will not be able to be observed. The secondary structure will not always be carried by the template molecules as DNA breathes in solution, the template molecules will be able to overcome the hindrance and fragments in the regions by the aids of some DNA polymerases. Thus, it will be terminated by the correct labeled nucleotide. The termination will be appeared as a reduced peak in the automated sequencing file. Q10. Perform a visual inspection of the following DNA sequence. Can you suggest an origin/function for this sequence? The presence of the CAAT box indicates that the short sequence is the promoter region with the coding sequence. There are also a few sites for the restriction enzymes in the sequence. Q11. You have a large linear fragment of dsDNA of unknown length. To determine the size, you have digested the fragment with three different restriction enzymes that have six base pair recognition sites and run the resulting fragments on the agarose gel. The three digests gave 5,6 and 7 fragments respectively. Having forgotten to run size markers you are forced to estimate the length of the original fragment (assuming a strictly random sequence). What is your estimate of the length in bps? Assume that the four nucleotides occurred randomly, the possibility of the occurrence of the recognition site for the restriction enzyme is 46=4096. Thus, for each 4096 bp, there will be a restriction enzyme. The restriction enzyme digested 5,6 and 7 fragments with an average of 6. Thus the sequence size will be 6 x 4096 = 24576bp. Q12. The sequence is 5CAATTTCACACAGGAAACAGCTACGACCATG 3. Q13. The experiment can performed following the procedures outlined follows: the gene of interest is inserted into the plasmid after it is isolated. The gene of interest is ligated into the plasmid as the vector for further step, the amplification. The plasmid is cut by using the same restriction enzymes used to cut the gene of interest. The EcoRI and SalI restriction enzymes aids the identification of the insert into the plasmid. The DNA segment will be ligated into the LacZ portion. The transformed plasmid will be grown on the X-gal plate. Colonies with white color indicate that the -galactosidase enzyme is not produced as the lacZ gene is disrupted. Contrast
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to the normal/undisrupted lacZ gene, the production of -galactosidase caused the cells to turn into blue color colonies. The white colonies will be isolated and extracted where the successful transformed recombinant cloned will be cloned and digested by using the restriction enzymes such as EcoRI, BglII and HindIII to produce different fragment sizes. The different fragment sizes can be separated by using agarose gel electrophoresis that will be loaded with the marker gene. At the same time, another set of digest is performed by using the original plasmid that is also digested by similar restriction enzymes. The results of fragmentations will be compared to each other (Glick and Pasternak, 2003). As the vector orientation is identified, the DNA sequencing can be performed to ensure that the size is 400bp. The fragmented plasmid is isolated and the agarose gel is purified. Next, methods such as chain termination, chemical degradation or automated sequencing can be performed to identify the sequences of the fragments. As only single stranded DNA is required, chain termination method is preferred. BglII and HindIII restriction enzymes will cut the 400bp fragments from 5 to 3 direction. The sequences obtained are shown as below: 5 AGATCT..AAGCTT 3 3TCTAGA...TTCGAA 5
References
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Brown TA., (2006). Gene cloning and DNA analysis: An Introduction 5th Ed. Blackwell Publishing, UK. Glick BR., and Pasternak JJ., (2003). Molecular Biology: Principles and Aplications of Recombinant DNA 3rd Ed. ASM Press, US. Sanger F., (1981). Determination of nucleotides sequences in DNA. Science, New Series 214:1205-1210 NCBI (National Center for Biotechnology Information) (2010) BLAST. URL: http://www.ncbi.nlm.nih.gov/blast/Blast.cgi Date accessed: 15th August 2010
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GEN 3040 DNA Sequencing

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GEN 3040

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Results Week 1: Preparation of Plasmid DNA Template for Automated Sequencing

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

999L H2O + 1L DNA 998L H2O + 2L DNA

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Figure 2. The gel electrophoresis of the digestion products

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Figure 5. The edited sequences from BioEdit

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Figure 6. The protein translation of the sequence by using BLAST program

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Figure 8. The Compute pI/Mw tool from Expasy

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Figure 9. The pOS2 consensus similarities

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

osmotin {promoter} [Nicotiana tabacum=tobacco, Genomic, 2051 nt] GenBank: S68111.1

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

N.tabacum ap24 gene GenBank: X65701.1 GenBank Graphics Related Sequences

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis

Plasmid isolation, automated DNA sequencing and bioinformatics analysis