Disease Histoplasmosis a.k.a. Darlings/ Cavers/ Spelunkers disease, North American Histoplasmosis Blastomycosis a.k.a.

North American Blastomycosis/ Chicagos/ Gilchrists Disease Coccidioidomycosis a.k.a. Posadas Disease/ San Joaquin Valley Fever/ California disease/ desert rheumatism

Etiologic agent Histoplasma capsulatum

Infectious particle Microconidia

Tissue form

Habitat

intraand Soil with bird or extra- cellular bat droppings yeast cells

Common Sites of Infection Lungs, bone marrow, liver, spleen

Histopath features

Culture at RT

Culture at 37C

small, round to oval large tuberculate small, singleintra- and extra- macroconidia budding yeast cellular yeast cells and cells small microconidia thick-walled yeast cells (8-15 m) with single, broad-based bud thick-walled spherules (10-80 m) filled with nonbudding endospores (2-5 m) yeast cells (10-30 m) with multiple, narrow-based buds (characteristic) Pyriform conidia borne singly on sepatate hyphae Double-walled yeast cells with single broadbased bud Not routinely done

Blastomycoses dermatitidis

conidia

broad-based yeast cells

Soil, rotten wood

Lungs, skin, long bones

Coccidioides immitis

arthroconidia

spherule

Soil in semi-arid regions

Lungs, skin, meninges

hyphae bearing barrel-shaped arthroconidia (24 x 3-6 m) separated by dysjunctor cells septate hyphae with atypical pattern of separation

Paracoccidioidomycosis Paracoccidioides conidia a.k.a. South American brazillensis Blastomycosis/ LutzSplendore-Almeidas Disease Penicilliosis marneffei Penicillium marneffei conidia

yeast cells with multiple, narrow-based buds

Soil, plants, fruit bats

Lungs, skin, mucous membranes

yeast cells with mariners/ steering wheel appearance

yeast cells with Isolated from soil transverse in the burrows of septum bamboo rats

Lungs, skin, liver, spleen, lymph noes, bone, bone marrow

small yeast cells (35 m), with transverse septum

Conidiophores branch into metullae that support the phialides that bear chains of conidia, colonies produce diffusible red pigment (very characteristic)

round to oval yeast cells with crosswalls

BY: PINKYRED