CITRATE BUFFER; PH 3.06.2, PKA = 6.

40 Citrate buffer (Gomori, 1955) stock

solutions: A: 0.1 M citric acid; B: 0.1 M sodium citrate. Use x ml A + y ml B and dilute to
100 ml with 50 ml DI.
0.1 M citric
acid
46.5
43.7
40.0
37.0
35.0
33.0
31.5
28.0
25.5
23.0
20.5
18.0
16.0
13.7
11.8
9.5
7.2

0.1 M
sodium
citrate
3.5
6.3
10.0
13.0
15.0
17.0
18.5
22.0
24.5
27.0
29.5
32.0
34.0
36.3
38.2
41.5
42.8

pH

3.0
3.2
3.4
3.6
3.8
4.0
4.2
4.4
4.6
4.8
5.0
5.2
5.4
5.6
5.8
6.0
6.2

PHOSPHATECITRATE BUFFER; PH 2.28.0, PKA = 7.20/6.40

Add the following to create 100 ml of phosphate/citrate buffer solution. Stock solutions
are
0.2 M dibasic sodium phosphate; 0.1 M citric acid (Pearse, 1980).
0.2 M
0.1 M citrate
pH
Na2HPO4 (ml) (ml)
5.4
44.6
2.6
7.8
42.2
2.8
10.2
39.8
3.0
12.3
37.7
3.2
14.1
35.9
3.4
16.1
33.9
3.6
17.7
32.3
3.8
19.3
30.7
4.0
20.6
29.4
4.2
22.2
27.8
4.4

23.3
24.8
25.7
26.7
27.8
29.0
30.3
32.1
33.1
34.6
36.4
40.9
43.6

26.7
25.2
24.3
23.3
22.2
21.0
19.7
17.9
16.9
15.4
13.6
9.1
6.5

4.6
4.8
5.0
5.2
5.4
5.6
5.8
6.0
6.2
6.4
6.6
6.8
7.0