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PREPARATION OF REAGENT : (i) Dilute 3ml of concentrated sulphuric acid and make up 100ml with distilled water. (ii) Dilute 20ml to 2000ml with distilled water.
2. LIMIT OF STRAYLIGHT : The absorbance of a 1.2-% w/v solution of potassium chloride at a path-length of 1cm should be greater than 2.0 at about 200nm when compared with water as reference liquid. 1.2% POTASSIUM CHLORIDE SOLUTION: Take 1.2 g of Potassium chloride previously dried at 105C for two hours into the 100ml volumetric flask. Add water to dissolve and make up to volume with water.
3. RESOLUTION POWER : Record the spectrum of a 0.02% v/v solution of Toluene in Hexane UV. The ratio of the absorbance at the maximum at about 269nm to that at the minimum at about 266nm is not less than 1.5 unless other wise specified in the monograph. 0.02% v/v TOLUENE: Take 0.02ml of Toluene into the 100ml volumetric flask. Add Hexane to volume.