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DNA REPLICATION

• Modes of DNA replication and proof


• Mechanism of DNA replication
• Replication of circular double stranded DNA in bacteria
- bacteria
• Replacation in circular single stranded DNA

- Virus ФX174
REQUIREMENTS FOR DNA REPLICATION

• 4 dNTPs – A, T, C dan G
• DNA template
• DNA polimerase III dan I
• Origin of replication Ori , A=T-rich region
• 5’ → 3’ elongation
• Bidirectional
• Concurrent for leading and lagging strands
• DNA ligase
• Girase dan helikase
• RNA primer
• Primase and primosome complex
• Single stranded binding protein / SSB

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