Universal Science College

(B. Sc. Biochemistry)

Third Semester 2005

Internal Examination Full Marks: 100

Subject: Biochem 215 (Proteins and Enzymes) Pass Marks: 45
Date: Feb 10, 2005 Time: 3 hours

Candidates are required to give their answers in their own words as far as practicable. The figure in the
margin indicates the full marks.
Group A
Long Questions (Any three) ×14 = 42)
(3×

1. Explain various types of enzyme inhibition. Illustrate the effect of inhibitions on Vmax and Km.
2. Describe the role of different proteins in muscular movement.
3. Describe coenzymes and their classification pattern. Describe the biosynthesis and biochemical functions of
Coenzyme A.
4. Discuss the binding of oxygen with hemoglobin and regulation there of.

Group - B

Short Questions: (Any six) ×7 = 42)

(6×

1. Describe the mechanism of enzyme catalysis

2. Describe the role of thiamine pyrophosphate and deficiency related disorders.
3. Define the role of folic acid and its structural analogs during therapeutic purpose.
4. Describe the models for positive cooperativitiy.
5. Describe the mechanism of chymotrypsin action.
6. Derive the Michaelis Menten equation
7. Describe the reaction involving Vitamin B12
8. Describe the L2 H2 model of immunoglobulin and its proteolytic cleavage

Group – C
Write short notes on: (Any eight) ×8 = 16)
(2×

1. Active center
2. Enzyme specificity
3. Zymogen
4. Structure of biotin
5. Bohr effect
6. Apoptosis
7. Effect of substrate in enzyme kinetics
8. Turnover number
9. MHC molecule
10. Glycogen phosphoreylase
11. Feedback inhibition
12. CD molecules

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