UNIVERSAL SCIENCE COLLEGE

(B.Sc. Biochemistry)
First Semester 2006
Second Term exam Full Marks: 100
Subject: Fundamentals of Biochemistry (Biochem. 115) Pass Marks: 45
Date: -13rd Feb. 2006 Time: 3 Hours
Candidates are required to give their answers in their own words as far as practicable. The figure
in the margin indicates full marks.
Group – A (Any Three) (3 X 14=42)
1. Describe the process of biological information transfer.
2. Describe briefly the general structure of the cell.
3. Describe weak interactions. Determine the weight in grams of sodium
dihydrogen phosphate (formula weight FW 138.01) and disodium hydrogen
phosphate ( FW 149.98 ) needed to prepare 1 liter of a standard buffer at pH
7.00 with a total phosphate concentration of 0.100 M. ( pK = 6.86 )
4. Discuss various types of chemical reactions occurring in a cell.
Group – B (Any Six) (6 X 7=42)
1. Discuss the coupling of catabolism and anabolism in a cell.
2. Discuss on the evolution of chloroplast and mitochondrial structures of the cell.
3. How would you describe the cytoskeletal structures of the cell?
4. Define Biological buffers.Discuss PH tritation curve
5. Define coligative property..What is the constration of OH - in a solution with an
H+ concentarion of 1.05 X 10-5 M?
6. (a) Glucose is the major energy-yielding nutrient for most cells. Assuming a
cellular concentration of 1 mM, calculate how many molecules of glucose
would be present in an eukaryotic cell. (Avogadro’s number, the number of
molecules in 1 mol of a nonionized substance, is 6.02 x1023. Assume that the
eukaryotic cell is spherical with a diameter of 50µm.)
(b) Hexokinase is an important enzyme in the metabolism of glucose. If the
concentration of hexokinase in our eukaryotic cell is 20 M, how many
glucose molecules are present per hexokinase molecule?
7. Calculate the pH of a dilute solution that contains a molar ratio of potassium
acetate to acetic acid (pKa = 4.76) of (a) 2:1; (b) 1:3; (c) 5:1; (d) 1:1; (e) 1:10.
8. Verify Oparins theory explaining Miller and Urey Experiment.
Group –C (Any Eight) (8 X 2=16)
1. The Endomembrane system
2. Viruses
3. Nucleus
4. Define ionic product of water
5. Define PK
6. Non Polar gass are poorly soluble in water. Why?
7. Genetic mutation
8. Structure of ATP
9. Draw the structure of Leucine and Cysteine
10. Stereospecifity
11. Transduction of energy
12. What are the criteria for a compound to be optically active?