1 AS 4276.

2—1995

Australian Standard
Water microbiology

Method 2: Culture media, diluents

and reagents

PREFACE

This Standard was prepared by the Standards Australia/Standards New Zealand Committee
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on Water Microbiology, FT/20, as part of a series of methods for the microbiological

examination of waters for domestic and industrial use.
This Standard is the result of a consensus among Australian and New Zealand
representatives on the Joint Committee to produce it as an Australian Standard.
The Standard provides, in part, information previously given in AS 1095.4.1.1— 1981,
Microbiological methods for the dairy industry —Methods for the examination of water
and air —Microbiological examination of water — Scope and general requirements.
The formulae of the media, diluents and reagents set out in this Standard have been drawn
from many sources and where possible a literature reference is given to provide more
detailed information about each medium and its use.

METHOD

1 SCOPE This Standard sets out the formulations, procedures and precautions to be
followed for the preparation of the culture media, diluents and reagents specified in this
series of standard methods for the microbiological examination of water.

2 REFERENCED DOCUMENT The following document is referred to in this

Standard:
AS
2243 Safety in laboratories (all Parts)

3 HAZARDOUS CHEMICALS Many chemicals are hazardous and care shall be

taken when handling all chemicals used to make up culture media, diluents and reagents.
Safety precautions and practices as detailed in AS 2243 shall be observed at all times.

4 REQUIREMENTS FOR INGREDIENTS

4.1 Agar
4.1.1 Description Agar for microbiological purposes shall be prepared especially for
this use and shall be identified as bacteriological agar. Such agar, when used at an
appropriate concentration in media, shall give satisfactory biological performance and
shall not cause significant inhibition to the growth of microorganisms. Information on the
gel strength, maximum moisture content and maximum inorganic matter content expressed
as ash shall be available from the manufacturer.

COPYRIGHT
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AS 4276.2-1995, Water microbiology Culture

media, diluents and reagents
This is a free 1 page sample. Access the full version at http://infostore.saiglobal.com.

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