Christopher Goyne Beloit College, WI Dr.

Susan Ustin Shane Grigsby

What is PROSPECT } How samples were taken } How water stress was calculated and estimated } PROSPECT Results } Water potential } Future Directions
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Use PROSPECT to estimate equivalent water thickness (EWT) } Compare EWT for stressed and unstressed plots } Compare EWT for AM and PM } Investigating water potential
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Radiative transfer model } Simulates leaf directional-hemispherical reflectance and transmittance } Inverted to simulate biological constituents
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Structure Pigment concentration Water content Dry matter content

Developed from generalized plate model Considers leaves as stacks of transparent parallel plates Assumes isotropic light flux

Leaf Area Meter Mass leaves Measure area of leaves Mass dried leaves Divide grams of water by leaf area (g/ cm^2)

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Measure reflectance and transmittance with integrating sphere. Prepare files Applied moving windows average from 1900nm 2500nm Run PROSPECT

Raw Spectrum

Averaged Spectrum

Before Smoothing

After Smoothing

Sample

Plot AM North

South

PM

North

South

Estimated EWT (g/cm^2)

Calc Es+ 348 0.01934431 321 0.0200809 313 0.01909737 325 0.02046713 316 0.02123818 326 0.02075515 310 0.02011689 301 0.01944199 308 0.020413 305 0.02009067 311 0.02089001 327 0.02036878 348 0.0187992 321 0.01991204 313 0.01980803 325 0.01998256 316 0.02066361 326 0.01992736 310 0.01962104 301 0.01930094 308 0.02042544 305 0.01917028 311 0.02038493 327 0.02027746

0.00902 0.009 0.00971 0.00951 0.01099 0.009 0.009 0.009 0.00953 0.00919 0.00934 0.009 0.00954 0.0097 0.00988 0.00866 0.01094 0.00822 0.00889 0.01032 0.01027 0.0097 0.00892 0.00987

Estimated vs. Calculated

Estimated EWT (g/cm^2) 0.012 0.011 0.01 0.009 0.008 0.0185 0.0115 0.0105 0.0095 0.0085 0.019 0.0195 0.02 0.0205 0.021 0.0215 R = 0.0471

Calculated EWT (g/cm^2)

AM vs. PM/ North vs. South

0.0115 0.0105 0.0095 0.0085 0.012 0.011 0.01

AM North AM South PM North PM South

0.009 0.008 0.01850.0190.0195 0.02 0.02050.0210.0215 Calculated EWT (g/cm^2)

Measure of plant stress } Measured with pressure bomb } More pressure required to see sap means high level of stress
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Plot Comparison
Time Water Potential (Mpa) 4AM 8AM 1PM -0.19257 -0.57133 -0.90306

Time North South S-N East West WE 8AM 8AM 8AM 8AM

MPa -0.5375 -0.60517 -0.52656 -0.61611

Time 1PM 1PM 1PM 1PM

MPa -0.84833 -0.95778 0.1094 -0.81278 -0.99333 0.189556

Determine if data can be estimated better with PROSPECT } PROSAIL

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Collect a larger range of data with greater degree of water stress and increase sample size

Links spectral variation in canopy with directional variation

What is PROSPECT } How samples were taken } How Equivalent water thickness (EWT) was calculated and estimated } PROSPECT Results } Water potential } Future Directions
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Dr. Susan Ustin } Shane Grigsby } Everyone from the land group } Emily } Rick Pasetto
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Rodriguez-perez, J. R., Riano, D., Carlisle, E., Ustin, S., & Smart, D. R. (2007). Evaluation of Hyperspectral Reflectance Indexes to Detect Grapevine Water Status in Vineyards [Electronic version].American Journal of Enology and Viticulture,58(3), 302-315. Feret, J., Francois, C., Asner, G. P., Gitelson, A. A., Martin, R. E., Bidel, L. P., & Ustin, S. L. (2008, February 23). PROSPECT-4 and 5: Advances in the leaf optical properties model separating photosynthetic pigments.Remote Sensing of Environment,112, 3030-3043. Retrieved July 16, 2012 Courault, D., Seguin, B., & Olioso, A. (2005).Irrigation and Drainage Systems,19, 223-249. Retrieved July 16, 2012