Code No: RR412311 Set No.

1
IV B.Tech I Semester Regular Examinations, November 2007
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Discuss in detail the +Ve and negative control of gene expression by citing the
example of Lac operon. [16]

2. Write about different enzymes required for the synthesis of CAMP. [16]

3. How do you apply the principle of metabolic engineering to the synthesis of Tryp-
tophan. Explain in detail. [16]

4. Discuss different parameters required for limiting end product accumulation. [16]

5. Write notes on [8+8]

(a) secondary metabolites

(b) Idiophase.

6. How does one carry out the bioconversion reaction for insoluble substrate. Discuss
in detail. [16]

7. Discuss different kinds of Bioconversions with suitable examples. [16]

8. Explain the phenomena of feed back repression in resistant mutants. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR412311 Set No. 2
IV B.Tech I Semester Regular Examinations, November 2007
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Discuss in detail the +Ve and negative control of gene expression by citing the
example of Lac operon. [16]

2. When there is a increase in the blood glucose level, how is carbohydrate metabolism
regulated? Discuss. [16]

3. Write notes on [8+8]

(a) Resistant mutants

(b) Limiting the accumulation of end products.

4. Explain the alteration in the phenomena of feed back inhibition in resistant mu-
tants. [16]

5. Comment on [16]

(a) Role of CAMP in catabolite regulation

(b) Role of end products in feed back regulation.

6. Discuss the regulation of enzyme synthesis (i.e. at genetic level). [16]

7. Comment on [8+8]

(a) Induction
(b) Gene dosage.

8. Discuss various methods to optimize and control of metabolic activities. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR412311 Set No. 3
IV B.Tech I Semester Regular Examinations, November 2007
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. What are branched pathways? How are these pathways regulated? Discuss in
detail. [16]

2. Describe the cycle for the synthesis of CAMP. [16]

3. Discuss in detail with an example where the synthesis of a primary metabolite be

optimized by using the principles of metabolic engineering. [16]

4. Write about aromatic amino acid biosynthesis pathway and its regulation. [16]

5. What are the precursor effects on primary and secondary metabolites production?
[16]

6. Comment on [8+8]

(a) Permeability
(b) Avoiding end product inhibition.

7. Write short notes on [8+8]

(a) Strain selection

(b) Growth cycle.

8. What do you understand by gene dosage? How would the benefit in biotechnological
process. [16]

⋆⋆⋆⋆⋆

1 of 1
Code No: RR412311 Set No. 4
IV B.Tech I Semester Regular Examinations, November 2007
METABOLIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
⋆⋆⋆⋆⋆

1. Write notes on [16]

(a) induction
(b) Catabolite repression
(c) Passive diffusion
(d) Facilitated diffusion.

2. Describe the cycle for the synthesis of CAMP. [16]

3. Compare and contrast between catabolic and feed back regulation. [16]

4. Discuss different parameters required for limiting end product accumulation. [16]

5. Write notes on [16]

(a) Prophase
(b) Idiophase.

6. Give examples of some products that are obtained by bioconversion. Write the
details of the process. [16]

7. Discuss different kinds of Bioconversions with suitable examples. [16]

8. How would you select an antibiotic producers. What are the possible ways in which
one would improve the strain producing a desired antibiotic? [16]

⋆⋆⋆⋆⋆

1 of 1