Datasheet: MCA497RT

Description: Specificity: Format: ProductType: Clone: Isotype: Quantity: F4/80

RATANTIMOUSEF4/80

Purified MonoclonalAntibody CI:A31 IgG2b 25g

ProductDetails
Applications Thisproducthasbeenreportedtoworkinthefollowingapplications.Thisinformationisderivedfromtestingwithin ourlaboratories,peerreviewedpublicationsorpersonalcommunicationsfromtheoriginators.Pleasereferto referencesindicatedforfurtherinformation.Forgeneralprotocolrecommendations,pleasevisit www.abdserotec.com/protocols.
Yes FlowCytometry ImmunohistologyFrozen ImmunohistologyParaffin(1) ImmunohistologyResin ELISA Immunoprecipitation WesternBlotting Immunofluorescence Radioimmunoassays ImmunoelectronMicroscopy No NotDetermined SuggestedDilution 1/501/100

Wherethisantibodyhasnotbeentestedforuseinaparticulartechniquethisdoesnotnecessarilyexcludeitsusein suchprocedures.Suggestedworkingdilutionsaregivenasaguideonly.Itisrecommendedthattheusertitratesthe antibodyforuseintheirownsystemusingappropriatenegative/positivecontrols. (1)Thisproductrequirespretreatmentofparaffinsectionspriortostaining.ProteinaseKisrecommendedfor tissuesfixedforlessthan24hours.CitratebufferpH6.0isrecommendedfortissuesfixedformorethan24 hours. Arecommendedprotocolfortheuseofthisantibodyonparaffinembeddedtissuescanbefoundat: www.abdserotec.com/support/recommendedprotocols667.html TargetSpecies ProductForm Preparation BufferSolution Preservative Stabilisers Approx.Protein Concentrations Immunogen ExternalDatabase Mouse PurifiedIgGliquid PurifiedIgGpreparedbyaffinitychromatographyonProteinGfromtissueculturesupernatant. Phosphatebufferedsaline

0.09% SodiumAzide

IgGconcentration1.0mg/ml

ThioglycollatestimulatedperitonealmacrophagesfromC57/BLmice.

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Links

UniProt: Q61549 EMR1_MOUSE Relatedreagents

EntrezGene: 13733 Synonyms FusionPartners Specificity Gpf480 SpleencellsfromimmunisedHOB2ratswerefusedwithcellsofthemouseNS1myelomacellline. TheF4/80antibodyrecognisesthemurineF4/80antigen,a160kDcellsurfaceglycoproteinthatisamemberofthe EGFTM7familyofproteinswhichshares68%overallaminoacididentitywithhumanEGFmodulecontainingmucin likehormonereceptor1(EMR1). ExpressionofF4/80isheterogeneousandisreportedtovaryduringmacrophagematurationandactivation.The F4/80antigenisexpressedonawiderangeofmaturetissuemacrophagesincludingKupffercells,Langerhans, microglia,macrophageslocatedinthegutlaminapropria,peritonealcavity,lung,thymus,bonemarrowstromaand macrophagesintheredpulpofthespleen.F4/80expressionhasalsobeenreportedonasubpopulationofdendritic cellsbutisabsentfrommacrophageslocatedinTcellareasofthespleenandlymphnode. TheligandsandbiologicalfunctionsoftheF4/80antigenhavenotyetbeendeterminedbutrecentstudiessuggesta roleforF4/80inthegenerationofefferentCD8+veregulatoryTcells. CloneCI:A31hasbeenreportedtomodulatecytokinelevelsreleasedinresponsetoListeriamonocytogenes,see Warschkau,H.&Kiderlen,A.F.fordetails.AbDSerotecrecommendtheuseofMCA497ELforthispurpose. AnantiidiotypicMCA497(CI:A31)antibodyHCA154A,isalsoavailablefromAbDSerotec.HCA154Aisdesignedasa controlantibodyforuseinconjunctionwithcloneCI:A31.ThisproductspecificallybindstoandblockstheF4/80 antibody. FlowCytometry References Use10ulofthesuggestedworkingdilutiontolabel106 cellsin100ul. 1.Austyn,J.M.andGordon,S.(1981)F4/80,amonoclonalantibodydirectedspecificallyagainstthemouse macrophage.Eur.J.Immunol.11:805815. 2.Hume,D.A.,Perry,V.H.,andGordon,S.(1984)TheMononuclearPhagocyteSystemoftheMousedefinedby ImmunohistochemicalLocalisationofAntigenF4/80:MacrophagesAssociatedwithEpithelia.TheAnatomicalRecord 210:503512. 3.Lee,S.H.etal.(1985)Quantitativeanalysisoftotalmacrophagecontentinadultmousetissues.Immunochemical studieswithmonoclonalantibodyF4/80.J.Exp.Med.161:475489. 4.Gordon,S.etal.(1992)Antigenmarkersofmacrophagedifferentiationinmurinetissues.Curr.Top.Micro. Immunol.181:137. 5.Warschkau,H.andKiderlen,A.F.(1999)Amonoclonalantibodydirectedagainstthemurinemacrophagesurface moleculeF4/80modulatesnaturalimmuneresponsetoListeriamonocytogenes.J.Immunol.163:34093416. 6.Lin,H.H.etal.(2005)ThemacrophageF4/80receptorisrequiredfortheinductionofantigenspecificefferent regulatoryTcellsinperipheraltolerance.J.Exp.Med.201:16151625. 7.Chan,R.J.etal.(2005)HumansomaticPTPN11mutationsinducehematopoieticcellhypersensitivityto granulocytemacrophagecolonystimulatingfactorBlood.105:37373742. 8.F.X.Pizzaetal.(2005)Neutrophilscontributetomuscleinjuryandimpairitsresolutionafterlengthening contractionsinmiceJ.Physiol.562:899913. 9.KathrynJMooreetal(2009)DivergentResponsestoLPSandBacteriainCD14DeficientMurineMacrophages. TheJournalofImmunology,165:42724280 10.Hemmi,H.etal.(2009)Anewtriggeringreceptorexpressedonmyeloidcells(Trem)familymember,Tremlike4, bindstodeadcellsandisaDNAXactivationprotein12linkedmarkerforsubsetsofmousemacrophagesand dendriticcells.JImmunol.182:127886. 11.Seitz,O.etal.(2010)WoundHealinginMicewithHighFatDietorobGeneInducedDiabetesObesity Syndromes:AComparativeStudyExpDiabetesRes.2010:476969. 12.Miao,E.A.etal.(2011)Caspase1inducedpyroptosisisaninnateimmuneeffectormechanismagainst intracellularbacteria.NatImmunol.11:113642. 13.Wang,X.etal.(2011)Activationofthecholinergicantiinflammatorypathwayamelioratesobesityinduced inflammationandinsulinresistanceEndocrinology.152:83646. 14.Cunningham,O.etal.(2009)Microgliaandtheurokinaseplasminogenactivatorreceptor/uPAsystemininnate braininflammation.Glia.57:180214. Emr1 Relatedreagents

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15.Tarallo,V.etal.(2011)Thebiflavonoidamentoflavoneinhibitsneovascularizationpreventingtheactivityof proangiogenicvascularendothelialgrowthfactors.JBiolChem.286:1964151. 16.Gornicka,A.etal.(2012)Adipocytehypertrophyisassociatedwithlysosomalpermeabilitybothinvivoandinvitro: roleinadiposetissueinflammation.AmJPhysiolEndocrinolMetab.303:E597606. Storage Storeat+4o Corat20o Cifpreferred. Thisproductshouldbestoredundiluted. Storageinfrostfreefreezersisnotrecommended.Avoidrepeatedfreezingandthawingasthismaydenaturethe antibody.Shouldthisproductcontainaprecipitatewerecommendmicrocentrifugationbeforeuse. 18monthsfromdateofdespatch. MaterialSafetyDatasheetDocumentation#10040availableat: http://www.abdserotec.com/uploads/MSDS/10040.pdf Forresearchpurposesonly

ShelfLife HealthAndSafety Information Regulatory

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RecommendedNegativeControls
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RecommendedUsefulReagents
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Otherformatsandapplications
ThisreagentisalsoavailableinaALEXAFLUOR488,ALEXAFLUOR647,ALEXAFLUOR700,APC,Biotin,FITC,LowEndotoxin,Pacific Blue,RPE,RPEALEXAFLUOR647,RPEALEXAFLUOR750,S/NformatandisusefulforFunctionalAssays

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