Title: The effect of different concentration of sodium chloride solution on a de-shelled of quails eggs.

Aim: To investigate the effect of different concentration of sodium chloride solution on the average of different in mass of quails eggs. Research Question: Does the different concentration of sodium chloride effects the average of different in mass of quails eggs that immersed in sodium chloride after 24 hours? Introduction:

Hypothesis:

Variables: Independent variable: Different concentration of sodium chloride solution, which are

Dependent variable: The average of different in mass of quails egg in 4 trials, ( Different in mass in 4 trials (trial 1 4 trial 2 trial 3 trial 4) ).

Controlled variable: No. 1. Controlled variables Volume and concentration of hydrochloric acid used. Methods to control the variables 350 of 2M hydrochloric are

poured little by little into a 500 beaker during the de-shell process.

2.

Volume of sodium chloride solution used.

70

of different concentrations of

sodium chloride are filled into each 150 beaker which, are beaker A,

beaker B, beaker C and beaker D and beaker E. 3. Type of eggs used. The quails eggs are used in this experiment. 4. Air humidity The humidity is controlled by closing the fan when do the weighing process to find the mass of quails eggs using electronic balance.

Table 1 showed the list of controlled variables and the method on how to control these variables.

Apparatus and Materials: No. 1. 2. 3. 4. 5. 6. 7. 8. 100 150 500 Apparatus measuring cylinder, ( beaker, beaker, Quantity(s) 1 5 1 1 3 1 3 1

Electronic balance, Spatulas Glass rod Paper towels Petri dish

Table 2 shows the apparatus that are needed in this experiment with their specific quantities.

No. 1.

Materials Sodium chloride solution with different concentrations which, are and 0 5 15 30

Quantity(s) of sodium chloride in beaker A, of sodium chloride in beaker B, of sodium chloride in beaker C, of sodium chloride in beaker D of sodium chloride in

and 45

beaker E. Each of the beakers is filled with 70 2. 2M of hydrochloric acid. 350 of sodium chloride.

Table 3 shows the materials with their quantities that are used in this experiment.

Procedures: 1. 5 quails eggs are placed into a 500 2. 350 beaker. of measuring

of 2M hydrochloric acid are measured by using a 100

cylinder. Then, the hydrochloric acid is poured into that beaker little by little. 3. The eggs are pushed down into the acid by using a spatula to ensure that all the eggs are not float above the surface of acid. 4. All the quails eggs are left into the beaker until all the shells got removed. 5. After the shells are removed, these eggs are rinsed carefully with the water. After that, the eggs are dried gently by patting them on paper towels. 6. After dried, the eggs are placed on the electronic balance and there initial mass are recorded in a suitable table. 7. Beaker A, beaker B, beaker C, beaker D and beaker E are filled with one quails egg each. 8. 70 of sodium chloride are poured into every beaker to cover the eggs. The

concentrations of sodium chloride that have been used are recorded. 9. The eggs were left in the beaker in about 24 hours. 10. After 24 hours, the sodium chloride is poured off the eggs. 11. The eggs are dried carefully by using a paper towel. 12. The eggs are placed again on the electronic balance and the masses are recorded in the table.

Data Collection Quantitative Data Concentration of sodium chloride, . Trial 1 Mass of quails eggs, g ( Initial Trial 2 Trial 3 Trial 4 Trial 1 g Final Trial 2 Trial 3 Trial 4

11.81 11.54 10.31 12.53 18.37 13.80 14.45 18.96 10.83 13.35 11.34 11.59 13.28 12.87 15.59 14.07 11.34 10.76 10.33 11.00 12.20 11.99 11.12 11.90 11.53 11.47 11.22 9.70 8.78 12.27 9.89 10.21 7.52 7.65 8.68 10.74 8.52

10.70 10.59 9.02

Table 4 shows the initial and final mass of quails eggs recorded before and after immersed in different concentrations of sodium chloride in every four trials. *Trial 1 is a data from our group. The rest of the trials are a pooled data.

Qualitative Data Before osmosis: 1. The surfaces of the quails eggs shells are smooth. 2. White foams are produced and half-filled the beaker when the of 2M

hydrochloric acid are poured little by little into the beaker-contained eggs. 3. Calcium carbonate is removed from the shells by the signing of greenish stain in the foams. 4. Some gas bubbles are seen around the eggs when the eggs started to remove its shells. However, the gas bubbles reduced since the de-shell process was complete. After osmosis: 1. The egg in the of sodium chloride was turgid and bigger in size compared

to other eggs. Its color also changed from white pale into a translucent yellowish surface. 2. The eggs in 5 and 15 of sodium chloride were less turgid. However, the

color was still remained white pale.

3. Both of the eggs that immersed in 30

and 45

of sodium chloride became

shrink but the egg in greater concentration more shrink. The color of the eggs turned into greenish white.

Data Processing 1. A sample calculation on the different in mass of quails egg for concentration of sodium chloride. (Initial mass Final mass) 18.37g( g( Trial 2 13.80g( 2.26g ( Trial 3 g( g( Trial 4 ( ( ) g) g) g) g) g) g) ) ( ) g( g) g( g) g)

Number of trial Trial 1

2. Sample calculation on the average of different in mass of quails egg when the concentration of sodium chloride used to immerse these eggs is 0%. Average Different in mass in (Trial 1 4 Average 6.56 ( ( ) ( ) 4. Trial 2 Trial 3 Trial 4)

3. Calculation on the standard deviation of the average different in mass of quails egg when immersed in the concentration of SD N SD 3 SD sodium chloride.

Presentation of Data Processing Concentration of sodium chloride, 0 5 15 30 45 Difference in mass of quails eggs, Trial 1 6.56 2.45 0.86 -1.64 -2.20 Trial 2 2.26 0.48 1.23 -1.26 -2.05 Trial 3 4.14 4.25 0.79 -1.26 -2.02 Trial 4 6.43 2.48 0.90 -1.53 -2.07 Average of difference in mass of quails eggs, 4.85 2.42 0.95 -1.42 -2.09

Table 5 shows the average different in mass of quails egg after immersed in different concentration of sodium chloride.

7 6 average of difference in mass of quail's egg,g 5 4 3 2 1 0 0 -1 -2 -3 concentation of sodium chloride,% 10 20 30 40 50

Graph 1 shows the average of difference in mass of quails egg,

in every four trials

when these eggs are immersed into different concentration of sodium chloride,%.

Discussion Based on the graph above, the patterns that revealed by the data resulting the graph to slope downwards from the peak until the lowest point in the graph. The peak point indicates that the average of difference in mass of quails egg is highest when the egg is immersed in the lowest concentration of sodium chloride, which is 0%.However, when the graph immersed in the highest concentration of sodium chloride which, is