Code: 9A23504 B.

Tech III Year I Semester (R09) Regular & Supplementary Examinations, November 2012 GENETIC ENGINEERING
(Biotechnology)

Time: 3 hours

Max Marks: 70

Answer any FIVE questions All questions carry equal marks ***** What are the steps involved in purification of genomic DNA from living cells. Add a note on manipulation of Purified DNA. (a) (b) (c) Describe the advantages and limitations of Gene therapy. Explain about RAPD and RFLP makers. Write about southern blot analysis. Explain in detail the PCR methodology. Add a note on applications of PCR technology. (a) (b) (c) (a) (b) (c) (a) (b) Write about C-DNA library construction. Describe gene amplication. Explain retroframposons. Explain the construction of PBR 322 vector. Describe RT PCR. Write about the applications of molecular markers. Describe the details of lactose operon model. Write briefly on different hyper of cloning vectors. Give an account of high level of expression of proteins in mammalian cells. (a) (b) (c) Describe Gene rearrangement. Explain restriction mapping of cloned fragments. Write about sigma switch in bacillus subtilis. *****

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Code: 9A23504 B.Tech III Year I Semester (R09) Regular & Supplementary Examinations, November 2012 GENETIC ENGINEERING
(Biotechnology)

Time: 3 hours

Max Marks: 70

1 2 (a) (b) (c)

Answer any FIVE questions All questions carry equal marks ***** Explain in detail different types of molecular markers. Add a note on their applications. Describe the method of identification of PCR product. Explain DNA hybridization. Write about methods of gene transfer. Describe the expression of cloned genes in yeast. (a) (b) (c) (a) (b) (c) (a) (b) Explain the mechanism of transposition and excision. Write briefly on repetitive DNA. Describe Arabinose operon model. Explain dot and sot blot analysis. Write about gene amplification. Explain briefly the enzymes involved in genetic engineering. Describe the steps involved in PCR methodology. Explain briefly novel techniques for generation of transgenic animals. Elucidate the mechanism of gene regulation in eukaryotes. (a) (b) (c) Write about applications of PCR technology. Describe restriction mapping. Explain sigma surface in bacillus subtlis. *****

3 4

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Code: 9A23504 B.Tech III Year I Semester (R09) Regular & Supplementary Examinations, November 2012 GENETIC ENGINEERING
(Biotechnology)

Time: 3 hours

Max Marks: 70

1 2 (a) (b) (c)

Answer any FIVE questions All questions carry equal marks ***** Define plasmids. Explain identification, classification and purification of plasmids. Write about the construction of PBR 322 vector. Describe gene amplification. Explain RFLP and AFLP. Elucidate the concept of gene therapy with an example of ADA. Add a note on their advantage limitation. (a) (b) (c) (a) (b) (c) (a) (b) Explain briefly steps involved in cloning of insulin. Describe methods of gene transfer. Write about bacterial transposons. Describe DNA methylation. Write briefly on novel techniques for generations of transgenic animals. Explain briefly sigma switch in bacillus subtitles Describe briefly gene regulation in eukaryotes. Explain Lactose operon model. Write the principle of PCR. Explain different types of PCR. Add a note on its applications. (a) (b) (c) Explain briefly refrotranposons. Write briefly western blot analysis. Explain gene rearrangement. *****

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Code: 9A23504 B.Tech III Year I Semester (R09) Regular & Supplementary Examinations, November 2012 GENETIC ENGINEERING
(Biotechnology)

Time: 3 hours

Max Marks: 70

1 2 (a) (b) (c)

Answer any FIVE questions All questions carry equal marks ***** Explain in detail the process and mechanism of gene regulation in eukaryotes. Write briefly tryptophan operon model. Describe southern blot analysis. Explain the mechanism of transposition and excision. Describe the expression of cloned gene in E.Coli. (a) (b) (c) (a) (b) (c) (a) (b) Write about multiplex PCR. Describe SNPs gene chip and microarray. What are the advantages of limitation in the generation of transgenic animals? Explain briefly repetitive DNA. Describe DNA sequencing. Write briefly about different types of cloning vectors. Write briefly on enzymes involved in genetic engineering. Describe host restriction in transfer of plasmids. Give an account of high level expression of proteins in yeast. (a) (b) (c) Describe promoter and enhancer elements in genelyulation. What methods are adopted to identify PCR product? Explain briefly the defection of transposition in bacteria. *****

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