A.H. Schmidt, V.I.

Nesterov

EXPERIMENTAL STUDY OF TORSION FIELD EFFECT ON LIFETIME.

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The goal of this study is to find out if there are any changes of an organisms lifetime after it was subjected to torsion field effect. For an obvious reasons it is not possible to carry out such study with humans. Theoretically such experiments are possible but in this case we will face some problems because such kinds of experiments take a lot of time. To answer this question the most suitable experimental models are mice of AKR inbred line, because these animals represent short-living line of mice. Their average lifetime is 9 months, but lifetime of non-linear mice is at about 2-3 years. Another peculiarity of these animals is that in 95% of females and 85% of males are subjected to development of spontaneous leucoses in the course of time. Thus experiments with torsion field effect on mice of AKR inbred line may answer to a question if such effect can prolong lifetime but also give us information if this effect influences clinical course of leucoses. On this ground we have carried out this study. According to latest scientific data torsion fields have principal meaning in organization, structure and functioning of living systems both in healthy condition and in cases of diseases. Inside and between cells permanent exchange of information by means of torsion waves is carried out. Functional disorders in a cell and in its chromosome apparatus arise when control processes are disturbed by intervention of pathological effects non-specific for an organism (radiation, toxins and carcinogens). In future these disorders lead damage or death of a cell, it its regular systems are not able to compensate them adequately.

Materials and methods of the research For generation of torsion field to which animals will be subjected we used highfrequency (with 40 GHz field generation frequency) torsion generator designed by A. Akimov G. Ignatiev, modified by the Institute of Practical Psychophysics (Omsk) with additional function of signal modulation to correlate torsion field oscillations with parameters specific to a living cell in order to support its regulatory mechanisms. Experiments were carried out on 67 mice of AKR inbred line of F 201-203 generations. Series of experiments were fulfilled in which blood values, clinical course of leucosis and lifetime of these mice subjected to torsion field were studied. Influence was brought by specially designed external oscillators to temporal regions of animals. Influence in accordance with noon-midnight was carried out before noon. To carry out research of blood and internal organs mice were decapitated, autopsied; leucocytes and red cells content in blood was determined in Gorjaev's count chamber, haemoglobins were determined by cyanhemoglobin method in blood smear stained by

Leishman's eosin methylene and azure-eosin according to. Romanovski, percentage of separate leucocytes forms was calculated. By the same method were stained smears imprints of thymus, spleen, liver and mesenteric lymph nodes in which quantity of blast elements in percentage related to hemopoietic cells was calculated. Blood of animals was centrifugated, plasma was separated and content of alanine aminotransferase (ALT) and aspartate aminotransferase (AST) according to Reitman and Frenkel was defined Results of research were statistically processed using Students criteria and Fisher formulas.

Results and discussion As previous researches show effect of modulated torsion field on animals may significantly increase their lifetime. We used a procedure when experimental animals were subjected to single impact by modulated torsion field within 3 minutes once a week. Screening group consisted of 21 females and 11 males, experimental group consisted of 23 females and 12 males. In these conditions in screening group 9 of 32 mice lived longer than average lifetime (279 days), in experimental group 3 times more: 29 of 35 mice. Difference according to Fisher is trustworthy P < 0.03. Also we witnessed one significant circumstance, the facts are the following. In researches when effect of various conditions on lifetime is studied, it is customary to compare lifetime of 10% of animals which appeared to be long-livers. In screening group it were 2 females and 1 male, one female lived for 367 days, other female lived for 378 days and male lived for 360 days. In experimental group there were 3 female which lived for 1006, 1064 and 1137 days! Average lifetime of long-livers in screening group is 368 11 days, in experimental group 1069 18 days, the difference is trustworthy - < 0,05. And another one observation. In blood of mice from experimental group a number of leucocytes and lymphocytes was significantly lower than in screening group. A number of leucocytes in mice from experimental group was equal to 7,18 1,032 109/l (n = 10), in screening group - 14,62 1,096 109/l (n = 8), a number of lymphocytes correspondingly 4,34 1,325 - 109/l (n = 10) and 9,78 0,656 - 109/l (n = 8), in both cases < 0,05. In other words effect of modulated torsion field slowed down leucosis leucomisation. During analysis of the experiment the following fact attracted out attention. Mice appeared to be very sensible to effect of modulated torsion field. In fact one sance of torsion field impact on organism of an animal was only 3 minutes long and one such sance once a week was enough to significantly increase lifetime and slow down leucosis leucomisation.

Conclusion. Carried out research has proven that effect of modulated torsion field may increase mice lifetime in 3 times and slow down leucosis leucomisation.