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CHAPTER III Methodology

This chapter presents the methods of research and procedure used in conducting this study. It includes the research design, procedure, materials and statistical analysis. Research Design The determination of the direct current voltage of Yellowfin tuna tail and seawater was done using electrochemical cells. There were three treatments, each of which different characteristics were measured.. The alternating current was then obtained by constructing a series circuit from the batteries prepared, then using it to trickle charge two SLA batteries, similar to the Uninterruptable Power Supply (UPS). This was then connected to an inverter that led out a 240 AC voltage. Materials and Instrumentation The study utilized various materials and instruments to effectively carry out the experimentation. Tuna tails, beakers, multimeter, copper wires, aluminum foil , electrical wire and battery terminals were utilized in this study. The research also made use of recyclable materials like plastic bottles, aluminum cans, scrap wood and old slippers. In addition, yeast and muscovado sugar were used for the fermentation process.

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Procedure Flow Chart

Collection of Tuna Tail

Testing on Different Household Appliances

Cost-Benefit Analysis

Autoclaving and Preparation of the Bottles

Measuring the Amperage

Statistical Analysis

Preparation of the Electrolytes

Asssembling the Alternating Current Series Circuit

Measuring the Direct Current Voltage

Constructing the Wet Cell Battery

Collection of Tuna Tails The tuna tails were taken from General Santos City Fishport. These tails were wastes of the Yellowfin tuna production in the said tuna plant.

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Autoclaving of Glass Bottles Collection and Preparation Six 1000 ml capacity glass bottles were collected. They were then washed thoroughly with soap and water to remove any dust particles or residue. The bottles were allowed to dry afterwards. Autoclaving The autoclaving was done at the Chemistry Laboratory of Mindanao State University, Tambler. The bottles were placed in the apparatus after wrapping them with paper and autoclaving cellophane. To ensure that the containers were sterilized, the autoclave was set to 15 psi for 15 minutes. It was made sure that the bottles were cooled down before conducting the next step in the procedure. Preparation of the Electrolytes The tuna tails were chopped into small pieces and placed in three of the autoclaved bottles containing 500 ml of distilled water each. Yeast and muscavado sugar were added to the each of the solution following the 1% and 10% percentage. The contents were then stirred thoroughly and covered with sterilized cotton plugs. All the containers were then placed in a cool dark area for 3 weeks. The fermented solutions were checked every day. The variables voltage was then measured after the time period herein. Seawater was also collected from Pearly Shells, a local coastline that has often been regarded as polluted. Three liters of the latter was reportedly gathered. Separating

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one liter from each electrolyte, the resulting mixture prepared by following a 3:1 ratio, respectively seawater and tuna tail. The different electrolytes were then immediately tested. Measuring the Direct Current Voltage Nine 150-ml beakers were washed and cleaned thoroughly. 120 ml of the different solutions were poured and a cathode and anode were placed in the beaker. These are namely the 3 by 1 aluminum strip and a copper rod. A mulitimeter was then utilized to measure the voltage of the different treatments. Results were then subjected to T-test. Construction of the Wet Cell Battery Eight plastic bottles of the same size and shape were collected from neighbors and dump sites. They were then rinsed with soap and water to eliminate dirt and residue. The top part was cut out using a kitchen knife. Recycled aluminum foil was then used to partially wrap the bottles (2 by 9 inches strip) Small wooden spacers ( by by 3) were cut from wood scraps. A glue gun was used to stick these spacers equally apart from each other inside the can. Small inch copper tubes were secured at specific wooden spacers. This was also ensured by utilizing the glue gun. Battery terminals, inch in diameter, were suspended by the latter and connected to a wire. Another wire was also attached to the aluminum foil. These small wires were expended by using it to exit out the current of the cathode and anode. To lock out any additional moisture, old slippers were cut to small circles to act as the lid of the can. A small exit hole was then made for the exiting cathode and anode.

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Fig 2.1. Top View of the Electrochemical Cell

Assembling the Alternating Current Series Circuit Eight assembled batteries were prepared for this part. Each of them contained a cathode and an anode. The positive was then connected to the negative pole and the rest of the batteries followed the same format of series circuits. The combined DC and the ampere per hour was thereafter measured using a multimeter. A 12 inch wire was then coiled around a nonconductor. It was made sure that this rheostat had spaces in between the wires looped. The resulting piece was then hid and protected using a small wooden box. This rheostat was then connected to the negative and positive currents through the wires.

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Fig 2.2 ACV Circuit Design

Two 6-volt sealed lead acid batteries (SLA) were then depleted. The latter were then put in a series circuit. Positive and negative wires delivered the modified current to the two batteries. Trickle charging was allowed for 3 hours. The aforementioned batteries were then measured for their DCV voltage as soon as the process was done. Afterwards, the wires were attached to their respective terminals on the inverter. They were then secured by small aluminum rings and small pieces of electrical tape. Measuring the Amperage The whole prototype, multimeter and a load (lightbulb) were then utilized in this testing. Using the ampere setting in the multimeter, it was connected to the specific terminals of the battery; namely positive and negative. After three successive tries, each spaced an hour apart, the average was then computed. Using this collected information, the amperes per hour was then solved for.

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Testing to Different Household Appliances A 9-watt light bulb was used to verify the electrical current the whole ACV possesses. Laptop chargers, a small radio and a 19 Color TV was also test ran. This was done by removing the major component in the inverter, for a more airy surrounding, and connecting it to an extension wire outside the prototype. Different designs were weighed and considered at this part. Cost Analysis To assess how practical the prototype was, a cost-benefit analysis was done. The costs and benefits of the ACV circuit were quantified. Benefits were obtained from the average monthly electrical bill for a Filipino household subtracted from the cost and distributed into different and specific time areas. Statistical Analysis Data on Measuring of the Direct Current Voltage (DC) were subjected to the analysis of Variance (ANOVA) to determine levels of significance. The obtained data were placed in a quantified table to show or evaluate the significance of differences between the two electrolytes and their resulting mixture.