Detection of Antibiotic Residues in Vinamilk

Kris Ferguson Regional Sales Manager Food and Crop Protection APAC

Why Test?

Page 1

Content

1.Example of a European milk quality control program 2.Problem of fast vs broad spectrum tests 3.Delvotest BLF how to use 4.Best practice when using Delvotest

Milk Residue Control : Example of the EU

STOP

Animals milk 1-40 Liters

CONTROL

Farms tank >>1,000 Liters

STOP CONTROL

STOP CONTROL

STOP

Truck >>10,000 Liters

CONTROL

Silo >>100,000 Liters

STOP CONTROL

FARM
Broad Spectrum test. Tests such as Delvotest SP NT or Delvotest T are capable of detecting all antibiotics in the milk in 2h30min to 3hours. Fast tests for -lactams. Tests such as Delvotest BLF are capable of detecting all antibiotics from the -lactam family in 5 minutes.

DAIRY

STOP CONTROL

Dairy Factory
STOP CONTROL

STOP CONTROL

Consumers products Yoghurt, Cheese, etc

Milk Residue Control : Example of the EU

STOP

Animals milk 1-40 Liters

CONTROL

Farms tank >>1000 Liters

STOP CONTROL

STOP CONTROL

STOP

Truck >>10,000 Liters

CONTROL

Silo >>100,000 Liters

STOP CONTROL

FARM
Farms will be fined if antibiotics are detected.

Tank sample is taken by the truck and sent to the lab

DAIRY

Laboratory >1,000 samples/day

Strong collaboration between Lab and Dairy. The Dairy organises and pays for the testing of the milk samples.

Dairy Factory
STOP CONTROL

Consumers products Yogurt, Cheese, etc

Good milk quality starts at the farm. Farmers are responsible for the delivery of good milk (price increases with quality).

Fast test and broad spectrum tests

The problem
Broad Spectrum test. Tests such as Delvotest SP NT or Delvotest T are capable of detecting all antibiotics in milk in 2h30min to 3hours.

STOP CONTROL

Based on Bacillus bacteria Sensitivity depends on the way antibiotics acts on the Bacillus.

STOP CONTROL

Fast tests for -lactams. Tests such as Delvotest BLF are capable of detecting all antibiotics from the -lactam family in 5 minutes.

Based on affinity of antibody or in general proteins with the antibiotic Sensitivity depends on the selectivity of the binding molecule to the antibiotics.

Those are different targets with different affinity for antibiotics different sensitivity profiles

Delvotest BLF

Incubator Use

Power on: press I/O

Display: Display PrE HEAt brd PressI /O and Program+ I/O to switch from broad spectrum test mode to fast test mode. Display 64Cand cont Press Program to change mode: Auto (Autoamtic switch off no sound) or Snd (sound is played after incubation) Press on Program to select desired mode and long press on Program to save the selected program. Time Set Press several times on Time Set to select the desired incubation time. (Time loops 1:45 to 3:15) Temperature set (63C 65C): Press Program together with Time Set to increase temperature. Press Program together with Start/Stop to decrease temperature.

Display: Display PrE HEAt FASt T=64C Display 64Cand Count Press Program to change timer: T1 2 means timer 1 : 2min Press on Time Set x times to increase T1 of x min. When time reached, long press on Time Set will save the new time. Press Program again to change timer 2: Same procedure as for T1. Temperature set (63C 65C): Press Program together with Time Set to increase temperature. Press Program together with Start/Stop to decrease temperature. Start Start count down (2min) Display remaining time Time elapsed Bip sound is played StRp is displayed Start /Stop to stop bip sound Start /Stop to start 3 min Start Stop 5sec

Start /Stop

Start /Stop Start Stop 5sec

Start count T=64C down Display remaining time Time elapsed Play Bip sound* Display Read AUTO Stop Heating** Start/Stop

Start count down (3min) Display remaining time Time elapsed Play Bip sound Display REAd Start /Stop

Stop 5sec

Delvotest Step by Step

Read the test. POS or NEG depending on colors (POS=Not good milk, NEG=Good milk) Incubate: the bacteria in the agar gel will grow if no antibiotics in the milk Take representative sample Cut of or remove ampoules from tray (!DO NOT DAMAGE OTHER PLATES or AMPOULES FOIL, if damaged, will be false positive!) Make a hole (Not necessary to remove foil for ampoules) Pipette milk (the correct volume of milk is the one in the pipette tube, not its tank)

Add the milk to the test The antibiotics is any will diffuse in the agar gel

Principle of Delvotest

Penicillin 4 POSITIVE RESULT Antibiotic free Sulfadiazine 100

NEGATIVE RESULT Color Time

Control Time

Control time versus fix reading time

Penicillin 4 POSITIVE RESULT Antibiotic free Sulfadiazine 100

NEGATIVE RESULT Color Time Fix time Test less sensitive than at control time If incubation time increases, the test sensitivity decreases

Control Time Fix time

Delvotest Colors
Wrong color reading Wrong results!

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Delvotest Colorcards for improved reading

Systematically in each box of Delvotest T

On request

On request

On request

Delvotest: Storage and Sampling

Good practice in storage/handling

The test is sensitive to LIGHT. Store in the DARK (in original packaging). The test is sensitive to TEMPERATURE. Store 4-8C (in original packaging). Place ampoules in fridge directly after cutting out the required number. Test should not freeze even partially. (creates milk runners and/or false positive, test can also become liquid) Up to 4 days, test can be transported at max 30C. The test is sensitive to AIR. Test where foil is removed can dry. (False positive / dried test) What if not stored correctly? Spores DIE (when freezing, agar gel looses structure) Test becomes slower and more sensitive False positives

Thank You!