Lora Jean Montalbo THE CULTURE MEDIUM PALCAM Agar Base COMPOSITION Peptone, Starch, Sodium Chloride, Yeast

Extract, Mannitol, Ferric Ammonium Citrate, Esculin, Dextrose, Lithium Chloride, Phenol Red, Agar Enzymatic Digest of Animal Tissue, Sterile Serum(if required) Yeast Enriched Peptone, Sodium Chloride Potato Infusion Agar Potatoes, Infusion from Solids, Beef Extract, Enzymatic Digest of Animal Tissue, Dextrose, Sodium Chloride, Agar Enzymatic Digest of Casein, Enzymatic Digest of Soybean Meal, Sodium Chloride, Phenylethanol, Agar Enzymatic Digest of Gelatin, Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Lactose, Bile Salts Mixture, Sodium Chloride, Neutral Red, Crystal Violet, Agar Milk Plate Count Agar Tryptone, Yeast Extract, Glucose, Antibiotic Free Skim Milk Agar Malt Extract, Agar Tomato Juice Solids , Enzymatic Digest of Casein, Peptonized Milk, Agar used for the selective isolation of pathogenic fungi from clinical materials Beef Extract, Enzymatic Digest of Casein, Dextrose, Agar Enzymatic Digest of Casein, Lactose, Bile Salts Mixture, Dipotassium used for the isolation and enumeration of microorganisms in milk and dairy products used for the cultivation of fungi For the cultivation of lactobacilli used for the cultivation of lactobacilli Enzymatic Digest of Soybean Meal, Dextrose, Agar, Cycloheximide, Chloramphenicol used for the cultivation and enumeration of microorganisms in dairy and bottled water products used for the detection of coliform bacteria at 37C and Escherichia coli Repared according to the formula used by Stockman and MacFadyean for the isolation of Brucella abortus. Brucellosis is a zoonotic disease with a domestic-animal reservoir used with blood for the selective isolation of Gram-positive cocci USES used with supplements as a selective and differential medium for the detection and isolation of Listeria monocytogenes from foods and environmental samples was developed for use in MIC susceptibility plates particularly against Mycoplasma bovis. This medium was intended for use with supplementation.

PPLO MIC Broth

Phenylethanol Agar

MacConkey Agar

used for the isolation and differentiation of Gram-negative enteric bacilli

Malt Agar Tomato Juice Agar

Mycobiotic Agar

Tryptone Glucose Extract Agar

EC Medium

Phosphate, Monopotassium Phosphate, Sodium Chloride Schaedler Agar Tryptic Soy Broth, Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, and Yeast Extract provide vitamins, nitrogen, and amino acids in Schaedler Agar. Dextrose is a carbon source. Tris(hydroxymethyl) Aminomethane is used to buffer the medium. Hemin (X factor) stimulates organism growth. L-Cystine is a reducing agent. Agar is the solidifying agent. Ammonium Dihydrogen Phosphate is the sole source of nitrogen in Simmons Citrate Agar. Dipotassium Phosphate acts as a buffer. Sodium Chloride maintains the osmotic balance of the medium. Sodium Citrate is the sole source of carbon in this medium. Magnesium Sulfate is a cofactor for a variety of metabolic reactions. Bromthymol Blue is the pH indicator. Organisms that can utilize Ammonium Dihydrogen Phosphate and Sodium Citrate as their sole sources of nitrogen and carbon will grow on this medium and produce a color change from green (neutral) to blue (alkaline). Agar is the solidifying agent. Tryptic Soy Broth, Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, and Yeast Extract provide vitamins, nitrogen, and amino acids in Schaedler Agar. Dextrose is a carbon source. Tris (hydroxymethyl) Aminomethane is used to buffer the medium. Hemin (X factor) stimulates organism growth. L-Cystine is a reducing agent. Agar is the solidifying agent. Enzymatic Digest of Casein and Proteose Peptone are the nitrogen sources in Universal Pre-Enrichment Broth. Sodium and Potassium Phosphates buffer the medium. Sodium Chloride, Magnesium Sulfate and Ferric Ammonium Citrate provide essential ions. Dextrose is an energy source, and Sodium Pyruvate helps stimulate the

at 44.5C used for the cultivation of anaerobic microorganisms

Simmons Citrate Agar

used for the differentiation of microorganisms on the basis of citrate utilization

Schaedler Agar

used for the cultivation of anaerobic microorganisms

Universal Pre-Enrichment Broth

used as the pre-enrichment for Salmonella testing of orange & apple juice, cider, cantaloupes, tomatoes, and mamey pulp according to FDA/BAM Methods

metabolism of stressed organisms. W-L Nutrient Medium Yeast Extract is a source of trace elements, vitamins, and amino acids. Enzymatic Digest of Casein provides nitrogen, amino acids, and carbon. Dextrose is a source of carbohydrate. Monopotassium Phosphate buffers the medium. Potassium Chloride, Calcium Chloride, and Ferric Chloride are essential ions and help to maintain osmotic balance. Magnesium Sulfate and Manganese Sulfate are sources of divalent cations. Bromcresol Green is a pH indicator. Agar is the solidifying agent. Enzymatic Digest of Casein and Enzymatic Digest of Gelatin provide nitrogen, amino acids, and minerals in used for the cultivation of yeasts, molds, and bacteria encountered in brewing and industrial fermentations

Wilkins-Chalgren Agar

used for the cultivation of anaerobic microorganisms

Wilkins-Chalgren Agar. Yeast Extract is added to provide vitamins and other growth factors, including purines

and pyrimidines. Sodium Chloride maintains the osmotic balance of the medium. Dextrose is a carbon source. L-Arginine contributes to growth of Eubacterium lentum. Sodium Pyruvate provides an energy source for Veillonella spp. and degrades hydrogen peroxide. Hemin (X Factor) and Vitamin K are essential for growth of a number of species of anaerobes. Agar is the solidifying agent. GC Agar Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue provide nitrogen, carbon, and minerals in GC Agar. Corn Starch absorbs any toxic metabolites produced. The Phosphates are buffering agents. Sodium Chloride maintains osmotic balance of the medium. Agar is the solidifying agent. Chocolate Agar is prepared from GC Agar with the addition of 2% Hemoglobin. Hemoglobin provides used with hemoglobin and enrichment for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms

Hemin (X factor) required for growth of Haemophilus and enhanced growth of Neisseria spp. A chemical enrichment composed of cofactors, vitamins, and nicotinamide adenine dinucleotide (NAD) are also required for growth of Haemophilus and Neisseria spp. If required, antimicrobial supplements are added as inhibitors for improved selectivity of the medium. Letheen Agar Base Enzymatic Digest of Casein and Beef Extract provide nitrogen, carbon, vitamins, and minerals in Letheen Agar Base. Dextrose is the fermentable carbohydrate. Lecithin neutralizes quaternary ammonium compounds and Tween 80 (Polysorbate 80) neutralizes phenols, hexachlorophene, formalin, and with Lecithin, ethanol. 3,4,5,6 Agar is the solidifying agent. Enzymatic Digest of Casein provides nitrogen, vitamins, minerals, and amino acids in Lauryl Tryptose Broth. Lactose is the fermentable carbohydrate for coliforms. Potassium Phosphates are the buffering agents, and Sodium Chloride is used to maintain the osmotic balance of the medium. Sodium Lauryl Sulfate is the selective agent used to inhibit non-coliform organisms. Enzymatic Digest of Casein, Yeast Extract, DextroseSodium Thioglycollate, Sodium Thiosulfate, Sodium Bisulfite, Polysorbate 80, Lecithin (Soybean), Bromcresol Purple, Agar D/E Neutralizing Broth Enzymatic Digest of Casein, Polysorbate 80, Yeast Extract, Dextrose, Sodium Thioglycollate, Sodium Thiosulfate, Sodium Bisulfite, Lecithin, Bromcresol Purple D/E Neutralizing Broth w/Tween Dextrose, Lecithin, Sodium For testing and neutralizing of For testing and neutralizing of antiseptics and disinfectants used with Tween 80 (Polysorbate 80) for the testing of quaternary ammonium compounds for antimicrobial activity

Lauryl Sulfate Broth

used for the detection of coliform bacteria in water and wastewater

D/E Neutralizing Agar

It is used for the isolation of microorganisms from sanitized environmental surfaces

Thiosulfate, Polysorbate 80, Enzymatic Digest of Casein, Yeast Extract, Sodium Bisulfite, Sodium Thioglycollate, Bromcresol Purple DC Medium with BCIG Lactose, Cefsulodin solution, Tryptose, Yeast Extract, Sodium Chloride, Proteose Peptone, Bile Salts, BCIG, Neutral Red, Agar Demi-Fraser Broth Base Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Beef Extract, Yeast Extract, Sodium Chloride, Disodium Phosphate, Monopotassium Phosphate, Esculin, Acriflavin, Nalidixic Acid, Lithium Chloride Deoxycholate Agar Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Lactose, Sodium Deoxycholate, Sodium Chloride, Dipotassium Phosphate, Ferric Citrate, Sodium Citrate, Neutral Red, Agar Pork Infusion Solids, Enzymatic Digest of Animal Tissue, Lactose, Sodium Citrate, Ferric Citrate, Sodium Deoxycholate, Neutral Red, Agar Dermatophyte Test Medium Enzymatic Digest of Soybean Meal, Dextrose, Phenol Red, Cycloheximide, Agar Enzymatic Digest of Casein, Dextrose, Bromcresol Purple, Agar

antiseptics and disinfectants

Used in the chromogenic differentiation of E. coli from other coliforms in water samples using the membrane filtration method

For use with ferric ammonium citrate for the selective enrichment of Listeria spp.

For the isolation and differentiation of Gram-negative enteric bacilli

Deoxycholate Citrate Agar

For the isolation and differentiation of Gram-negative enteric bacilli

For the selective isolation of dermatophytic fungi

Dextrose Tryptone Agar

For isolation of mesophilic or thermophilic spoilage microorganisms in food

Dextrose Tryptone Broth

Enzymatic Digest of Casein,

For cultivation of mesophilic or

Dextrose, Bromcresol Purple

thermophilic spoilage microorganisms in food

Dichloran Glycerol (DG-18) Agar Base

Peptone, Glucose, Monopotassium Phosphate, Magnesium Sulfate, Zinc Sulfate, Copper Sulfate, Dichloran, Chloramphenicol, Chlortetracycline, Agar Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Sodium Chloride, Deoxyribonucleic Acid, Agar Enzymatic Digest of Animal Tissue, Glucose, Monopotassium Phosphate, Magnesium Sulfate, Rose Bengal, Dichloran, Chloramphenicol, Agar Enzymatic Digest of Casein, Lactose, Bile Salts Mixture, Dipotassium Phosphate, Monopotassium Phosphate, Sodium Chloride

For the selective isolation and enumeration of yeasts and molds from foods

DNase Test Agar

For the differentiation of microorganisms on the basis of deoxyribonuclease activity

DRBC Agar

For the selective isolation and enumeration of yeasts and molds from foods

EC Medium

For the detection of coliform bacteria at 37C and E. coli at 44.5C

EC Medium w/ MUG

Enzymatic Digest of Casein, Lactose, Bile Salts, Dipotassium Phosphate, Monopotassium Phosphate, Sodium, 4-Methylumbelliferyl-b-DGlucuronide Enzymatic Digest of Casein, Lactose, Bile Salts Mixture, Dipotassium Phosphate, Monopotassium Phosphate, Sodium Chloride

For the fluorogenic detection of Escherichia coli

EC Medium, Modified

For use with novobiocin for the selective pre-enrichment of E. coli O157:H7

EC Medium, Modified with Novobiocin

Enzymatic Digest of Casein, Lactose, Sodium Chloride, Dipotassium Phosphate, Monopotassium Phosphate, Bile Salts, Novobiocin Desiccated Ox Bile, Enzymatic Digest of Gelatin, Sodium Phosphate, Dibasic, Dextrose, Potassium Phosphate, Monobasic, Brilliant Gree

For the selective enrichment of E. coli O157:H7

EE Broth, Mossel

For the cultivation and enrichment of Enterobacteriaceae in food

Eosin Methylene Blue Agar

Enzymatic Digest of Gelatin, Lactose, Sucrose, Dipotassium Phosphate, Eosin Y, Methylene Blue, Agar Enzymatic Digest of Gelatin, Lactose, Dipotassium Phosphate, Eosin Y, Methylene Blue, Agar

For the isolation and differentiation of Gram-negative enteric bacilli

Eosin Methylene Blue Agar, Levine

For the isolation and differentiation of Gram-negative enteric bacilli

Fastidious Anaerobe Agar

Peptone, Sodium Chloride, Soluble Starch, Sodium Bicarbonate, Glucose, Sodium Pyruvate, LCysteine HClH20, Sodium Pyrophosphate, L-Arginine, Sodium Succinate, Hemin, Vitamin K, Agar Enzymatic Digest of Casein, Yeast Extract, Dextrose, Lystine, Sodium Chloride, Sodium Thioglycollate, Resazurin, Agar

For the cultivation of anaerobic microorganisms

Fluid Thioglycollate Medium

For use in sterility testing. Conforms to USP

Fraser Broth

Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Beef Extract, Yeast Extract, Sodium Chloride, Disodium Phosphate, Monopotassium Phosphate, Esculin, Lithium Chloride Sheep blood

For use with acriflavin, nalidixic acid and ferric ammonium citrate for the selective enrichment of Listeria spp.

Chocolate agar

culturing fastidious organisms such as Haemophilus species and Neisseria

Bacillus Cereus Agar Base

Enzymatic Digest of Casein, Egg Yolk Emulsion, Mannitol, Polymyxin B, Sodium Chloride, Magnesium Sulfate, Disodium Phosphate, Monopotassium Phosphate, Bromthymol Blue, Sodium Pyruvate, Agar Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue , Yeast Extract, Sodium Chloride, Dextrose, Sodium Bisulfite, Agar Enzymatic Digest of Animal Tissue, Lactalbumin Hydrolysate, yeast Extract, Sodium Chloride, Hemin, Sodium Pyruvate, a-Ketoglutamic Acid, Sodium Metabisulfite, Sodium Carbonate

isolation and presumptive identification of Bacillus cereus

Brucella Agar

For the cultivation of Brucella spp. and other fastidious microorganisms

Campylobacter Enrichment Broth

Used with antimicrobics for the selective enrichment of Campylobacter spp.

Clostridium Difficile Agar

Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue,Pork Brain Heart Infusion, Fructose, Disodium Phosphate, Monopotassium Phosphate, Magnesium Sulfate, Sodium Chloride, Agar Enzymatic Digest of Animal Tissue, Beef Extract, Yeast Extract, Dextrose, Sodium Acetate, Polysorbate 80, Potassium Phosphate, Ammonium Citrate, Magnesium Sulfate, Manganese Sulfate Yeast Extract, L-Cysteine, Charcoal, Activated Ferric Pyrophosphate, sterile ACES Buffer, a-Ketoglutarate, Agar Eosin and methylene blue

use with cycloserine and cefoxitin for the isolation of Clostridium difficile

Lactobacilli MRS Broth

For the cultivation of lactobacilli

Legionella Agar

For the isolation of Legionella spp.

Eosin methylene blue (EMB) agar

inhibits the growth of Gram-positive bacteria and provides a color indicator distinguishing between organisms that ferment lactose and those that do not For the selective enrichment of Listeria spp.

Listeria Enrichment Broth

Enzymatic Digest of Casein, Enzymatic Digest of Soybean Meal, Yeast Extract, Dextrose, Sodium Chloride, Dipotassium Phosphate, Cyclohexamide, Acriflavin, Nalidixic Acid Enzymatic Digest of Animal Tissue, Ticarcillin, Sodium Pyruvat, Bile Extract, Ammonium Sulfate, Ferrous Ammonium Sulfate Magnesium Sulfate, Dipotassium Phosphate, Monopotassium Phosphate, Phenol Red, Crystal Violet, Agar Enzymatic Digest of Gelatin, Glycerol, Magnesium Chloride, Potassium Sulfate, Irgasan, Agar

Pseudomonas Cepacia Agar

For the selective isolation of Burkholderia cepacia

Pseudomonas Isolation Agar

For the isolation of Pseudomonas aeruginosa and other Pseudomonas spp.

Salmonella Shigella Agar

Beef Extract, Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Lactose, Bile Salts, Sodium Citrate, Sodium Thiosulfate, Ferric Citrate, Brilliant Green, Neutral Red, Agar Enzymatic Digest of Gelatin, Cefsulodin, Enzymatic Digest of Casein, Novobiocin, Enzymatic

For the isolation of Salmonella spp. And some strains of Shigella spp.

Yersinia Selective Agar

For use with cefsulodin and novobiocin for the isolation

Digest of Animal Tissue, Yeast Extract, Mannitol, Sodium Deoxycholate, Sodium Cholate, Sodium Chloride, Sodium Pyruvate, Magnesium Sulfate, Neutral Red, Crystal Violet, Irgasan, Agar Bile Esculin Azide Agar Enzymatic Digest of Casein, Yeast, Enriched Meat Peptone, Oxbile, Sodium Chloride, Sodium Citrate, Ferric Ammonium Citrate, Esculin, Sodium Azide, Agar Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, Liver Digest, Yeast Extract, Sodium Chloride, Agar Brain Heart Infusion, Enzymatic Digest of Gelatin, Dextrose, Sodium Chloride, Disodium Phosphate Nutrient Broth No. 2, Campylobacter Supplement, Charcoal, Cefoperazone, Casein Acid Hydrolysate, Sodium Desoxycholate, Ferrous Sulfate, Sodium Pyruvate, Agar Enzymatic Digest of Gelatin, Glycerol, Magnesium Chloride, Potassium Chloride, Cetrimide (Cetyltrimethylammonium Bromide, Agar Pork Infusion Solids, Enzymatic Digest of Animal Tissue, Lactose Sodium Citrate, Ferric Citrate, Sodium Deoxycholate, Neutral Red, Agar Enzymatic Digest of Soybean Meal, Gentamicin, Dextrose, Chlortetracycline, Phenol Red, Cycloheximide, Agar Enzymatic Digest of Casein, Dextrose, Bromcresol Purple Agar Corn Meal, Infusion from solids, Agar

of Yersinia enterocolitica

For the selective isolation and differentiation of Group D streptococci

Blood Agar Base

For use with blood for the isolation and cultivation of a wide variety of fastidious microorganism

Brain-Heart Infusion Broth

For the cultivation of a wide variety of fastidious microorganisms

Campy Blood-Free Selective Medium

For use with cefoperazone for the selective isolation of Campylobacter spp.

Cetrimide Agar

For use in the isolation and identification of Pseudomonas aeruginosa

Deoxycholte Citrate Agar

For the isolation and differentiation of Gram negative enteric bacilli

Dermatophyte Test Medium

For the selective isolation of dermatophytic fungi

Dextrose Tryptone Agar

For isolation of mesophilic or thermophilic spoilage microorganisms in food For the cultivating of phytopathological fungi and production of chlamydospores by Candida albicans

Corn Meal Agar

Reinforced Clostridial Medium

Peptone, Beef Extract, and Yeast Extract provide nitrogen, vitamins, and amino acids

Reinforced Clostridial Medium is used for the cultivation of anaerobes from food and clinical specimens.

R2A Agar

Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, and Acid Hydrolysate of Casein provide nitrogen, carbon and minerals in R2A Agar. Yeast Extract is a source of vitamins and trace elements. Dextrose serves as a carbon source in the formula. Soluble Starch aids in the recovery of injured organisms by absorbing toxic metabolic by-products. Enzymatic Digest of Casein and Casein Acid Hydrolysate are carbon and nitrogen sources used for general growth requirements in MSRV Medium. Sodium Chloride maintains the osmotic balance in the medium.

R2A Agar is used for the enumeration and cultivation of bacteria from potable water.

Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified

Rappaport-Vassiliadis (MSRV) Medium Semisolid Modified is used with Novobiocin for the rapid detection of motile Salmonella spp.

Rappaport-Vassiliadis Salmonella Enrichment Broth

Soy Peptone is the carbon and nitrogen sources for general growth requirements in RappaportVassiliadis Salmonella Enrichment Broth. Magnesium Chloride raises the osmotic pressure in the medium, and Potassium Phosphate acts as a buffer. Malachite Green is inhibitory to organisms other than Salmonella spp.

Rappaport-Vassiliadis Salmonella Enrichment Broth is used for the enrichment and selective isolation of Salmonella spp. Conforms to Harmonized USP/EP/JP Requirements.

Rappaport-Vassiliadis R10 Broth

Enzymatic Digest of Casein is the carbon and nitrogen sources for general growth requirements in RappaportVassiliadis R10 Broth. Columbia Blood Agar Base contains Enzymatic Digest of Casein, Enzymatic Digest of Animal Tissue, and Yeast Enriched Peptone providing nitrogen, carbon, amino acids, and vitamins. Ferric Ammonium Citrate aids in the differentiation of Listeria spp. Since all Listeria spp. hydrolyze esculin, the addition of ferric ions to the medium

Rappaport-Vassiliadis R10 Broth is used for the selective enrichment of Salmonella spp. from foods.

Oxford Listeria Agar Base

Oxford Listeria Agar Base is used with antimicrobics for the selective isolation of Listeria spp.

will detect the reaction. Oxbile (Oxgall) Oxbile is used as a selective agent for the isolation of Gram-negative microorganisms, inhibiting Grampositive bacteria. The major composition of Oxbile is taurocholic and glycocholic acids. Orange Serum Agar Enzymatic Digest of Casein provides carbon and nitrogen sources for general growth requirements. Orange Serum provides the acid environment favorable to recovering acid-tolerant microorganisms. Yeast Extract provides essential vitamins to stimulate the growth of yeasts and molds in OGYE Agar Base. Orange Serum Agar is used for the cultivation of aciduric microorganisms associated with spoilage of products. Oxbile (Oxgall) is dehydrated bile for use in preparing microbiological culture media.

OGYE Agar Base

OGYE Agar Base is used with oxytetracycline in the detection and isolation of yeasts and molds from foods.

Dextrose is the carbon energy source. Agar is the solidifying agent.

The supplement, Oxytetracycline, is a selective agent used to inhibit bacterial growth. Lysine Iron Agar Enzymatic Digest of Gelatin provides carbon, nitrogen, and amino acids required for good growth of a wide variety of organisms. Yeast Extract provides vitamins and cofactors required for growth, and additional sources of nitrogen and carbon. Dextrose is an energy source. LLysine is the substrate used to detect lysine decarboxylase and lysine deaminase enzymes. Ferric Ammonium Citrate is an indicator of hydrogen sulfide production. The nitrogen, amino acids, and carbon sources are provided by Enzymatic Digest of Casein. Vitamins and certain trace elements are supplied by Yeast Extract. Sodium ions for transport and osmotic balance are provided by Sodium Chloride. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue Lysine Iron Agar is used for the differentiation of microorganisms on the basis of lysine decarboxylase and hydrogen sulfide production.

LB Broth, Miller

LB Broth, Miller is used in molecular genetic studies.

Letheen Broth with Tween, Modified

Letheen Broth with Tween, Modified is used for the recovery of

provide nitrogen and carbon, essential for good recovery of a wide variety of bacteria and fungi. Littman Agar Enzymatic Digest of Gelatin provides nitrogen, amino acids, vitamins, and carbon required for organism growth in Littman Agar. Dextrose is included as an energy source. Oxgall restricts the spreading of fungu It contains bile salts (to inhibit most Grampositive bacteria, except Enterococcus and some species of Staphylococcus), crystal violet dye (which also inhibits certain Grampositive bacteria), neutral red dye (which stains microbes fermenting lactose), lactose and peptone. The medium contains enzymatic digests of casein and soybean meal which provides amino acids and other nitrogenous substances making it a nutritious medium for a variety of organisms. Dextrose is the energy source.

microorganisms from cosmetics.

Littman Agar is used for the isolation and cultivation of fungi.

MacConkey (also McConkey) agar

MacConkey (also McConkey) agar is a culture medium designed to grow Gram-negative bacteria and stain them for lactose fermentation.

Trypticase soy agar

Trypticase soy agar is a bacterial growth medium.

Hektoen enteric agar (HEK or HE or HEA)

HE contains indicators of lactose fermentation and H2S production; as well as inhibitors to prevent the growth of gram positive bacteria.

HE agar is designed to isolate and recover fecal bacteria belonging to the Enterobacteriaceae family. HE is particularly useful in isolating Salmonella and Shigella XLT4 Agar is used with Tergitol 4 for the isolation of non-typhi Salmonella spp.

XLT4 Agar

Enzymatic Digest of Animal Tissue is the source of complex nitrogen compounds in XLT4 Agar. Yeast Extract is added as a source of vitamins and cofactors. Differentiation of Salmonella from other organisms that also grow on this medium is based on fermentation of Xylose, Lactose and Sucrose, decarboxylase of Lysine, and the production of hydrogen sulfide. Hydrogen Sulfide production is detected by the addition of ferric ions. Sodium Yeast Extract provides sources of nitrogen, carbon, and vitamins

XLD AGAR

XLD Agar is used for the isolation and differentiation of enteric

required for organism growth. Xylose, Lactose, and Sucrose, provide sources of fermentable carbohydrate.

pathogens.

Lowenstein - Jensen Medium

L-Asparagine and Potato Flour are sources of nitrogen and vitamins in Lowenstein-Jensen Medium. Monopotassium Phosphate and Magnesium Sulfate enhance organism growth and act as buffers. Glycerol and the Egg Suspension provide fatty acids and protein required for the metabolism of mycobacteria. Enzymatic Digest of Gelatin provides nitrogen, amino acids, vitamins, and carbon required for organism growth in Littman Agar. Dextrose is included as an energy source. Enzymatic Digest of Gelatin is the source of carbon, nitrogen, vitamins, and minerals in Violet Red Bile Agar w/ MUG. Yeast Extract supplies Bcomplex vitamins that stimulate bacterial growth. Enzymatic Digest of Casein and Enzymatic Digest of Animal Tissue are used as a nitrogen and vitamin source in this medium. Dextrose and Mannitol are the fermentable carbohydrates. The melting point of a 12% concentration of gelatin is between 28 and 30C, allowing it to be used as a solidifying agent. Certain microorganisms elaborate gelatinolytic enzymes (gelatinases) which hydrolyze gelatin, causing liquefaction of a solidified medium. Gelatin is also used as a nitrogen and amino acid source. GC Agar is employed as a basal medium in the preparation of Chocolate Agar, Thayer-Martin Medium,

Lowenstein - Jensen Medium is used with fresh egg and glycerol for the isolation and differentiation of Mycobacterium

Littman Agar

Littman Agar is used for the isolation and cultivation of fungi.

Violet Red Bile Agar W/ MUG

Violet Red Bile Agar W/ MUG is used for the detection of coliforms and the fluorogenic detection of

GN Broth (Hajna)

GN Broth (Hajna) is used for the selective enrichment of Gramnegative organisms.

Gelatin

Gelatin is a protein source and solidifying agent for use in preparing microbiological culture media.

GC Agar

GC Agar is used with hemoglobin and enrichment for the isolation and cultivation of Neisseria gonorrhoeae and other fastidious organisms.

Modified Thayer-Martin Medium,

Martin-Lewis Agar, and Transgrow Agar. Violet Red Bile Agar Enzymatic Digest of Gelatin is the source of carbon, nitrogen, vitamins, and minerals in Violet Red Bile Agar. Violet Red Bile Agar is used for the enumeration of coliforms in food and dairy product conforms to American Public Health Association (APHA).