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Department of Genetic Engineering School of Bioengineering SRM University BT0202 Molecular Biology

Instruction # Do not write or scribble on the question booklet. Score on the coding sheet. # Return the question booklet along with the coding sheets. # Do not copy.
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1. The phosphate group is joined to a ------------ sugar a) Deoxyribose b) oxyribose c) 3deoxyribose d) 2deoxyribose 2. The 3 indicates the carbon present in a) Sugar b) Base c) Phosphate d) all the above 3. The base is attached to the sugar at position a) 1 b) 2 c) 3

d) 4

4. The bond between sugar and base is called a) peptide bond b) diester bond c) sugar bond d) glycosidic bond 5. A. Sugar and base is called as a nucleoside B. Sugar , base and glycosidic bond is called as nucleotide a) A true, B true b) A true, B false c) A false, B true d) A false, B false 6. Nucleoside is formed with the release of a) energy b) water

c) 2Pi

d) oxygen

7. The sequence 5-AGCTAAGCT-3 in DNA is equivalent to which sequence in RNA. a) UCGAUTCGA b) UCGUUTCGA c) UCGAUUCGA d) UCGUUUCGA 8. Which of the following correctly describes A form DNA? a) Right handed, antiparallel, double helix with 10 bp/turn b) left handed, antiparallel, double helix, with 12 bp/ turn c) Right handed, antiparallel, double helix with 11 bp/turn d) Globular form single stranded nucleic acid 9. RNA moleculas differ from DNA in all but which of the following aspects? a) kinds of purines b) kinds of pyrimidines c) number of strands per molecule d) Type of sugar

Rex Arunraj, Department of Genetic Engineering, School of Biotechnology, SRM University

10. Which of the following is not a characteristic of most DNA under physiological conditions? a) A pairs with T by 2 H bonds b) antipolarity of complementary chains c) 3.4 A pitch d) 10 bp/turn of the helix 11. The polarity of DNA chain is represented by a) 3-5 b) 1-6

c) 1-3

d) 5-3

12. The class of RNA molecules to which aminoacids are covalently bound is a) mRNA b) rRNA c) tRNA d) mtRNA 13. Adenosine is a a) Nitrogenous base c) Ribonucleotide 14. A. % A= % T, % C = % G B. A # U, G # C a) A true, B true c) A false, B true 15. [ A + T] / [ C + G ] is --------------------a) Zero c) not always one

b) ribonucleoside d) deoxyribonucleotide

b) A true, B false d) A false, B false

b) one d) none of the above

16. Which of the following is not a terminator codon a) UGA b) UAA C) UAG 17. Which of the following is not true for a B form of DNA a) 10 bp/ turn b) right handed helix c) glycosidic bond anti at C and syn at G d) vertical rise 3.2 A 18. The DNA can exist in uncommon form like a) C b) D 19. Z conformation was named because a) Phosphate groups are zigzag b) form present under extreme conditions c) alternating purine and pyrimidines d) all the above 20. In Z DNA the base pairs are flipped a) 450 b) 900 21. Z form is less stable because c) 1800

d) UGG

c) D

d) none of the above

d) none of the above

Rex Arunraj, Department of Genetic Engineering, School of Biotechnology, SRM University

a) they are formed under extreme conditions b) they have alternating purines and pyrimidines c) phosphate groups are closer to each other d) number of bp is more per turn 22. Which is not true for the stability of Z form of DNA a) cytosine methylation makes it stable b) formation of negative supercoiling c) shallow major groove and deep minor groove d) alternating purines and pyrimidines 23. The helix pitch is a) 450 b) 900 c) 1800 d) 3600

24. mRNA cap is formed by methylation of Guanine at position a) 1 b) 3 c) 5 25. In a prokaryotic ribosome ------ is not a characteristic feature a) ribosome is 70 S b) Large subunit is 60S and small subunit is 30 S c) r RNA are 16 S, 5 S, and 23 S d) they are present in the cytoplasm 26. Which of the following is not true for major groove a) it contains H bond donors b) it contains van der Waals forces c) H bond acceptor d) covalent bond

d) 7

27. Which of the following molecules functions to transfer information from the nucleus to the cytoplasm? a) DNA b) mRNA c) tRNA d) proteins 28. mRNA will form hybrids only with the coding strand of DNA because a) DNA will not reanneal at high temperatures b) denatured DNA will not reanneal after it is diluted c) RNA:DNA hybridization follows the base-pairing rules d) DNA will not reanneal at low temperatures 29. The regions of DNA in a eukaryotic gene that encode a polypeptide product are called a) hnRNAs b) exons c) leader sequences d) introns 30. What is added to the 3'-end of many eukaryotic mRNAs after transcription? a) introns b) a poly A tail c) the trinucleotide 5'-CCA d) cap

Rex Arunraj, Department of Genetic Engineering, School of Biotechnology, SRM University

31. A messenger acid is 336 nucleotides long, including the initiator and termination codons. The number of amino acids in the protein translated from this mRNA is a) 333 b) 336 c) 111 d) 112 32. Frederick Griffith accidentally discovered transformation when attempting to develop a vaccine for pneumonia. He injected mice with samples from S-strain (virulent) and/or R-strain (nonvirulent) pneumococci bacteria (Streptococcus pneumoniae) . Which of the following results is NOT consistent with Griffith's experiments? a) injected S-strain; mouse dies. b) injected R-strain; mouse lives. c) injected heat-killed S-strain; mouse lives. d) injected mixture of heat-killed S-strain and live R-strain; mouse lives. 33. Which scientists first gave experimental evidence that DNA is the genetic material? a) Avery, MacLeod, and McCarty who repeated the transformation experiments of Griffith, and chemically characterized the transforming principle. b) Garrod, who postulated that Alcaptonuria, or black urine disease, was due to a defective enzyme c) Watson and Crick who gave a model for the structure of DNA d) Hershy and Chase experiments 34. In a double stranded molecule of DNA, the ratio of purines : pyrimidines is: a) variable b) determined by the base sequence in RNA c) always 1:1 e) determined by the purines in the sense strand of DNA 35. Which of the following molecules does not form part of DNA? a) purine b) pyrimidine c) deoxyribose d) amino acid 36. The transcription of DNA to a molecule of messenger RNA occurs: a) on the ribosomes b) in the cytosol c) in the nucleus d) only during cell division 37. Codons are composed of a) triplet sequences of nucleotide bases in mRNA b) triplet sequences of nucleotide bases in DNA c) triplet sequences of amino acids in polypeptide chains d) triplet sequences of deoxyribose sugars in DNA 39. If, in Griffith's experiment, a mixture of heat-killed smooth bacteria and living rough bacteria were injected into a mouse, it would be expected to __________. a) kill the mouse b) cause transfection c) cause transduction d) have no effect on it 40. If Hershey and Chase found only 35S in the bacterial cells, they would conclude that __________. a) protein is the genetic material b) DNA and protein together serve as the genetic material

Rex Arunraj, Department of Genetic Engineering, School of Biotechnology, SRM University

c) DNA is the genetic material d) no conclusion is possible 41. RNA differs from DNA in all of the following ways except __________. a) thymine b) Sugar c) Functions d) 5 3 orientations of strand 42. Which of the following is true of transfer RNA? a) It is responsible for correct positioning of amino acids in a polypeptide. b) It serves as the site of protein synthesis. c) It functions in transcription, translation and replication. d) It is an important component of ribosomes. 43. Which of the following is most similar to the Watson-Crick helix? a) B-DNA b) A-DNA c) C-DNA d) Z-DNA 44. Which of the following is NOT a characteristic of DNA? a) antiparallel b) composed of nucleotides c) complementary d) contains ribose 45. A:C base pairing is not feasible not because of the following reason a) H bond acceptor of adenine lie opposite to H bond acceptor of cytosine b) No place for an water molecule c) H bond donor of adenine lie opposite to H bond donor of cytosine d) structural difference 46. Which of the following contain most of the chemical information for interaction a) major groove b) minor groove c) Phosphate group d) Sugar 47. Which of the following is a characteristic feature of T:A base pair in the major groove a) MADA b) ADAM c) AADH d) HDAA 48. Which of the following do not describe B-DNA - the Watson-Crick Model a) Major groove wide and deep b) Minor groove narrow and deep c) anti glycosidic bonds d) DNA under dehydrating conditions. 49. Hoogsteen pairing is best described by a) form triplex DNA b) long stretches of either purines or pyrimidines c) involves N-7, N6 and O6 atoms of the purine ring d) all the above 50. DNA duplexes can be melted or denatured by a) temperature b) pH c) ionic strength

d) All the above

Rex Arunraj, Department of Genetic Engineering, School of Biotechnology, SRM University