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RT. Pawar et al.

, IJSID, 2012, 2 (5), 466-470



International Journal of Science Innovations and Discoveries

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Department of Zoology, Majalgaon Arts, Science and Commerce College, Majalgaon, Dist. Beed. (M.S.) India. R. T. PAWAR

Received: 24-09-2012 Accepted: 14-10-2012

*Corresponding Author

ABSTRACT The present study communication deals with the study of histopathological changes region. The intestinal tissue recognized was parasitized by ptychobothridae cestode occurred in the intestinal tissue of Mastacembelus armatus collected from Marathwada belonging to genus Senga Sp (Dollfus 1934). Here the cestode parasite having armed management side. Histopathology. epithelium of host tissue. The worms are having pathogenic nature and are harmful to

scolex adheres to intestinal tissue with the help of scolex and cause damages to villi, fishes and have got importance in aquaculture point of view i.e. the fish disease Address: Name: RT. Pawar Place: Majalgaon Arts Science and Commerce College, Majalgaon, MS, India E-mail: Key words: Ptychobothridae Cestode parasites, Mastacembelu armatus, Senga and



International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012


RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 diseases and disorders. Naturally it is important to study this relationship, not because of their parasitological value but for the relative existence of mankind. These studies may have considerable intrinsic interest and raise fundamental questions, common to other areas of biology, at a molecular, cellular, tissue and whole organism level. be suitably adopted to the morphology, physiology, biochemistry, immunology and ecology of its hosts. genetically systems, namely those of the parasite, its intermediate and its definitive host. Thus if a cestode, has to survive, must the pathogenesis of larval cestodes in fish, but little is known of the reactions in the invertebrates hosts, and many adult parasite tapeworms have been studied, for their histopathology. For example in Pseudophylleidea, Bothriocephalus Echeneibothrium, Phyllobothrium and Acanthobothrium. Sircar and Sinha (1980) have also studied the histopathology of Lytocestus indicus. In this fist investigation we studied the histopathology of fish by cestode from Mastacembeleus armatus. dissected out the intestine. Some of the intestines were infected and some were not. The worms were collected washed in saline solution, flattened, preserved in 4% formalin and later processed for taxonomical studies. The infected intestine with point (58-60oC). For the histopathological study, the freshwater fish, Mastacembelus armatus were brought to the laboratory and MATERIALS AND METHODS gowkongensis was studied by Kortings, (1977). In fishes Mcvicar (1972) described host parasite relationship of Review on the pathogenesis of adult cestodes has been made by Rees, G. (1967). There is also extensive literature on The host parasite relationship in cestodes is complex one, involving interactions between at least two or more Thus the host parasite relationship results in the gain of one organism and the loss of another and leads to various INTRODUCTION

Never the less quit of few reports on the pathogenicity of cestode on fishes are available i. e (Sindernann 1970, Bylund 1972).

cestode parasites on closer observation identified as genus Senga Sp (Dollfus 1934). The worms, which were attached to Fixed tissues were washed, dehydrated through alcoholic grades, cleared in xylene and embedded in paraffin wax with melting observed under the microscope for histopathological study. Blocks were cut at 8m and slides were stained with Haematoxylin: eosin stain. Best slides were selected and RESULTS AND DISCUSSION

intestine, were kept intact and small pieces of such intestines and other healthy intestine were fixed in Bouins fluid fixative.

and all layers i.e. serosa, muscularis mucosa, submucosa and mucosa are clearly observed (Fig. I).

through the tissues and cause damages to villi, epithelium of host tissue. In the longitudinal section seen that the worm are approaching to the intestinal villi. The mucosal layer of intestine and invades the host tissue, the worm is not only successful to enter into the intestine forming the ulceration in the intestinal wall; causing damage to the host tissue (Fig. II). The parasites embedded in the infesting of fibrocytes, lymphocytes, plasma cells and macrophages.

The normal histological structure (Healthy intestine) of the host Mastacembelus armatus showed that the healthy villi The histopathology of infected intestine with the cestode Senga Sp. is showing penetrative type of scolex, invades

were either free in the intestinal lumen of mixed with necrotic debris consisting of mucosal epithelium and monocular cells or

International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012


RT. Pawar et al., IJSID, 2012, 2 (5), 466-470


Fig. III & IV showing the magnified transverse section of penetration of parasites up to muscularis layer and entangled

Fig. I- T. S. of normal histological architecture of the host M. armatus

Fig. II- L.S. of infected intestine showing damaged area, penetrative type of scolex and adhered of scolices.

International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012


RT. Pawar et al., IJSID, 2012, 2 (5), 466-470

can be concluded that the worms, Senga Sp. finds the food material and other favorable necessary requirement for its

nourishment and growth from the host tissue by causing damage to the intestinal tissue of host. This result is in accordance with the Mackiewicz (1972). However, the helminthes crosses majority of the intestinal layers (internal epithelium, definitive host (C. J. Hiware,2008 ) view i.e. the fish disease management side. The worms are having pathogenic nature and are harmful to fishes and have got importance in aquaculture point of submucosa,muscularis layer) and come to lie near serosa suggesting that, it is very dangerous and destructive parasites to the

Fig. III & IV- T. S. of Infected intestine showing the penetration of parasites and entangled scolices. (Magnified)

It is essential to study in detail the hosts nutrition other aspects in relation to parasitic interaction but from above it

International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012


RT. Pawar et al., IJSID, 2012, 2 (5), 466-470 Aurangabad, India for providing necessary laboratory and library facilities during tenure of this work. 1. 2. 3. 4. 5. 6. REFERENCES societies scientatium fennica 58.1-11 232-246. The authors are thankful to the Head, Department of Zoology, Dr. Babasaheb Ambedkar Marathwada University, Acknowledgement

Bylund ,G (1972) : Pathogenic effects of Diphyllabathriid plerocercoid on its host fishes commentationes biologicae, Dollfus (1934) Sur uncestode Pseudophyllidae parasite de poisson ornament Bull. Sac. Zool. France 69; 476-490. elasmobranch, Raji naevus. Parasitology, 65(1): 77-88. Anim. Res., 14(1): 53-56.

Mackiewicz, J.S. (1972): Relationship of pathology of scolex morphology among caryophyllid cestodes. F. Parasitkde, 39(3): Mcvicar, A.H. (1972): The ultra-structure of the parasite host interface of three tetraphyllidean tapeworms of the Rees, G. (1967): Pathogenesis of adult cestodes. Helm. Abst. , 36: 1-23. Sindermann C.J. (1970) :principal diseases of marine fishes and shellfish academic press New York 369 pp. Lytocesus clariasae Jadhav and Gahvane, 1991Journal of Yala Rajabhat University

7. Sircar, M. and Sinha, D.P. (1980): Histomorphology of Lytocestus indicus infection in the fish Clarias batrachus. Indian Soc. J. 8. Hiware C.J & Pawar R.T., (2008): Studies on Histopathology of Clarias batrachus (Linnaeus) Intestine Parasited by Cestode,

International Journal of Science Innovations and Discoveries, Volume 2, Issue 5, September-October 2012