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What is a Mutation?
permanent change in the DNA sequence Range from single DNA base to a large segment of a chromosome
Types of Mutations
Point mutations
single nucleotide base changes in a gene's DNA sequence
- Missense mutations - Nonsense mutations - Silent mutations
RNA
Translation
Protein
Mutant Analysis
Known as forward genetics Phenotype-centric process Reliable means to assign gene function Enormous effort to identify each gene responsible for a particular phenotype
Mutant Analysis
Reverse genetics Prediction of function based on DNA sequence Required large collections gene-indexed mutation
Mutant Analysis
Forward Genetics
Select a biological process Generate highly redundant mutant populations Screen for mutants with a desired phenotype Map and clone the gene responsible for the phenotype
Reverse Genetics
Select a gene or genes of interest Generate highly redundant mutant populations Develop and conduct sequence-based mutant screens Analyse the phenotype of the mutants
EMS Mutagenesis
Efficient mutagens in plant - Soybean, pea, Lotus japonicus, Medicago truncatula Generate a broad range of DNA alteration - Point mutation Unbiased distribution of mutation
Supernodulation mutant
EMS
Wild type
nts1007
Wild type
Examples
wild type
FN37
Biological agents
DNA or RNA molecules as mutagen Introduction of engineered DNA/RNA molecule into living cell. - Agrobacterium tumefaciens as shuttle - Plant-associated bacteria - DNA/RNA viruses - Particle bombardment
Examples
Workshop on Mycorhizal