High Performance Liquid Chromatography Questions 1. 500 ppm retention time = 170.1 s 100 ppm retention time = 272.

1 s 200 ppm retention time = 170.7 s 300 ppm retention time = 171.9 s Average time= 196.2 s 2. 500 ppm theophyline = 142.9 s 3. 250 ppm Theobromine = 135.9 s 4. No, the 100 ppm caffeine solution is significantly different than all other caffeine samples. 5. The retention times were similar to their original solutions; however, they were not exactly the same. The error was up to a 9 point variance. 6. Yes, the separate solutions all have significantly different retention times, which prevents overlap. Also, the solutions did not affect the retention times compared to the original retention times in a significant manner. 7. Our unknown, the Roundys brand tea, was similar to the caffeine standard. The retention times were comparable to the caffeine retention times, except for the 100 ppm caffeine standard. 8. We assumed that there was no overlap in the caffeine part of the tea sample and that the Roundys Brand Teas contains caffeine. 9. Yes, the plot is reasonably linear. The equation y = 60.423x - 35.531 is the linear equation trend line. The R2 value for this equation = 0.9597, which is a reasonable value to demonstrate linearity. 10. The caffeine concentration is 240 ppm of caffeine in the Roundys Tea sample. This was found substituting the peak height of the tea into the linear trend line for caffeine. 11. C= (4.552/8.318)*(500 ppm) = 274 ppm of caffeine. This is 274-240 =34 ppm different, which is comparable. 12.