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This experiment is conducted to achieve a few objectives, which are to determine the Chromium (VI) content which present in simulated lake water sample using a spectrophotometer, to demonstrate the proper method of diluting solution to prepare a series of standard solutions in the range of 1 to 100 parts per million (p.p.m) to be used in the calibration of the spectrophotometer, and thus, to anal yze whether the simulated lake water sample tested is suitable for drinking water and agricul ture purposes.

The lake water sample used to test for presence of chromium is believed to be contaminated as the lake is situated near a roadway where road users with automobiles use it every day. As mentioned before, rivers, lakes and any water streams which are near to congested areas have the highest risk or most susceptible to contain heavy metals such as chromium itself and is then classified as contaminated water source.

According to the values of concentration of chromium (VI) which is apparentl y calculated to contain 49.8 parts per million, this obviousl y means that there is a significant content of chromium ions in the water. Based on the consideration of state and federal regulatory agencies, natural waters are regarded to be toxic if the conc entration of Chromium (VI) is any higher than 0.100 parts per million. The lake water sample tested is then concluded to be unsafe and unhygienic for drinking water for public consumption or even agricultural purposes.

However, a few steps might be done erroneousl y which results in such an outrageous value of chromium (VI) content. First and foremost, the techniques applied to measure such a small volume of diluting substance might not be suitable. This might lead to difficulties when measuring the volum e of chromium (VI) needed to dilute the standard solutions.

Besides that, wrong way of holding the square cuvette bottle used to contain the diluted solutions might also lead to errors. Any fingerprints or smudge imprinted on the outer surface of the cuv ette can become an unfavourabl y interference for the solutions to absorb wavelengths of light in the spectrophotometer. Thus, the reading of absorbance by the instruments may not be accurate.

In addition, the experiment conducted to determine the absorba nce value of diluted solutions that contain chromium (VI) is done onl y once. Thus, no average value can be obtained from the results, and so the accuracy of the value taken is not much convincing.

Others, the square cuvette bottle might not be cleansed p roperly with distilled water each time before it is used for another attempt. This will lead to inaccurate contents of either standard solutions or the chromium solution.


The concentration of Chromium (VI) in the lake water sample tested is 49.8 parts per million. Since it is more than 0.100 parts per million as what has been standardized by state and federal regulatory agencies, the water sample is concluded to be contaminated and thus, not suitable for drinking or agricultural purposes .