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Ari S. Akerstein, M.S.

AriAkerstein@gmail.com
3860 26th st, #4, SF CA 94131 415.902.6171

SUMMARY: Molecular Genetics focused research with experience in cell culture. Bring a
wide breadth of knowledge with eagerness to expand current skills, learn new
experimental methods and contribute to a team environment.

SUMMARY OF ACCOMPLISHMENTS:
• Recognized as the first SFSU graduate student to conduct thesis research in
both the Molecular Biology and Mathematics departments
• Master’s thesis validated a novel algorithm characterizing protein kinetics
involved in radiation treatment in cancer cells
• Awarded patent, successfully negotiated $600,000 licensing agreement for
Akrowheels fitness product

RESEARCH EXPERIENCE:
9/07-8/08 Molecular / Computational Biology Researcher
San Francisco State University
Department of Cell & Molecular Biology, Mathematics
• Customized a Master’s program with research in both the Cell / Molecular
Biology, Mathematics departments
• Implemented Monte-Carlo simulations to analyze and image protein kinetics,
outperforming standard methods
• Validated algorithm performance by comparison to previously published data
sets using advanced Excel analytics, Matlab
• Prepared and presented talks, posters at professional scientific conferences, lab
meetings and journal clubs
• Thesis title: Simulating DNA repair mechanisms using stochastic, reaction-
diffusion methods

10/07-08/08 Volunteer Laboratory Researcher


University of California San Francisco (UCSF)
Department of Orthopedic Surgery (Lotz Tissue Engineering Lab)
• Prepared media / reagents for experiments and cell culture
• Collaborated with Post Doctoral fellow to develop ultrasound techniques
inducing stem cell differentiation
• Developed strong sterile technique, good lab practices
• Assisted development of three dimensional cell matrices to induce stem cells to
cartilage regeneration
• Performed RNA extraction & isolation, cell-count assays, RT-PCR analysis as
well as cell culture passaging

09/06-09/07 Post-Baccalaureate Laboratory Researcher


San Francisco State University
Department of Cell/Molecular Biology (Fuse Lab)
• Conducted Experiments to elucidate endocrine mechanisms behind molting
• Performed ICC/ICH staining, microscopy to determine hormone levels and
signaling during various larval stages of development
• Performed patch clamp electrophysiology experiments on motor neurons to
determine signaling patterns
• Performed various dissections for electrophysiology experiments
PUBLICATIONS / PRESENTATIONS
• Akerstein, A., Bernstein, D. and Arsuaga, J. An in silico approach to analyzing
nuclear FRAP protein recovery using an extension of the Gillespie algorithm, (In
Press) Biochemistry and Cell Biology (2009)
• Akerstein, A., Bernstein, D. and Arsuaga, J.Using the Gillespie Algorithm to
investigate the role of chromatin geometry in the recruitment of repair proteins to
DNA double strand breaks. Abstract, Biochemistry and Cell Biology, (2007)
• Akerstein, A., Bernstein, D., and Arsuaga, J., Analysis of FRAP using an
extension of the Gillespie algorithm. Abstract, Biochemistry and Cell Biology,
(2006)
• Presenter, 28th annual Asilomar Chromatin & Chromosomes Conference
(2006), Using reaction-diffusion methods to simulate gamma-H2AX signaling of
repair proteins in response to DNA double strand breaks

SKILLS:
LABORATORY
Patch Clamp Electrophysiology, Cell Culture (stem cells: Human, Murine), Gel
Electrophoresis, RNA extraction & isolation, Immunoc staining, small animal
dissection, sterile technique, media preparation, cell viability assay, RT-PCR
analysis, Analysis of Fluorescence Recovery after Photobleach (FRAP),
Extremely organized documentation & lab notebook

COMPUTATION / ANALYSIS
MS Office, Excel, SAS (modeling, data analysis), Word, PowerPoint, Matlab,
gene annotation (using Apollo), Expert analyzing data using Excel, NCBI
BLAST, WU BLAST, Database queries for bioinformatics (e.g. GenBank,
Flybase), Phylogenetic modeling, Unix, Matlab, Strong problem solving ability,
Strong writing and interpersonal skill

EDUCATION:
San Francisco State University, San Francisco, CA
MS, Molecular Biology, Aug 2008
Tested and validated a novel algorithm characterizing protein kinetics
fundamental to DNA repair in irradiated cells for Master’s Thesis.
Customized a Master’s program with research in both the Cell / Molecular
Biology and Mathematics departments

Boston University, Boston, MA,


BA, International Relations, concentration in Economics July 2000

PROFESSIONAL SOCIETIES
AAAS, K-stars marathon club, SF Debate, Long Now Foundation

REFERENCES:
Available upon request

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