Gilson Product Guide

Gilson, Inc.
Table of Contents
Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions for todays demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendary Gilson Pipetman products to a full range of system solutions, Gilson is the element of purification for your laboratory. Gilsons innovative products will revolutionize the lab; providing the durability, flexibility, modularity and ease-of-use the industry has come to expect from Gilson. Gilson has worldwide sales and service supporting the pharmaceutical, biotech, environmental, clinical, food and forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through GPC clean-up. Gilson focuses on early drug discovery and development with our preparative HPLC systems. Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic markets performing a wide variety of clean-up applications. Gilson offers complete solutions including the revolutionary control software, TRILUTION. TRILUTION LC Software is a complete HPLC software package designed specifically to meet purification needs. TRILUTION LH Software is designed to make automation of liquid handling and SPE applications simple and dependable. Gilson, Inc. world headquarters is located in Middleton, WI and is responsible for research, development and manufacturing of all Gilson instrumentation and software in addition to providing direct sales to the U.S. Gilson S.A.S., located outside of Paris, France, is responsible for all development and manufacturing of Gilson pipetting products and benchtop laboratory products. Gilson solutions are sold and supported worldwide.
HPLC: Systems
2 10
Solid Phase Extraction Systems 35 39
Quality GX-281Policy Preparative HPLC System ......................................... 5 GX-271 ASPEC, with 406 Single Syringe Pump...........36 Analytical to Semi-preparative GX-271 ASPEC, with 406 Dualthe Syringe Pump ..............36 ItGX-281 is Gilson, Inc.s policy to promote a total commitment to quality by each individual throughout HPLC System ........................................................................... 6 GX-274 ASPEC, with two 406 Dual Syringe Pumps ...37 Gilson organization worldwide. It is Gilsons goal that this commitment to quality will result in GX-271 Preparative HPLC System ......................................... 7 Quad-Z 215 Liquid Handler for SPE ...................................38 complete customerto satisfaction with both Gilsons products GX-271 Analytical Semi-preparative HPLC System ... 8 and services. SPE 215 System .........................................................................39 845ZPREP Semi-preparative to Code 300 Series Racks ............................................................39 ISOPreparative 9001 Certification HPLC System.................................................... 9 Gilson is committed to quality, and it shows in................... everything from Handlers design to manufacturing, Liquid 40 47 cLC Analytical to Preparative HPLC System 9 Gilson does GX-271 Fraction Trapping System ......................................10 technical support and training. Due to this commitment to quality GX-281 and customer Gilson Liquid satisfaction, Handler ...........................................................41 GX-271 Handler ...................................42 has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Preparative Gilson S.A.S.Liquid are both HPLC: Liquid Handlers/Injectors 12 16 GX-271 Analytical Liquid Handler ......................................43 ISO 9001 certified. GX-281 Liquid Handler/Injector ..........................................13 GX-271 Liquid Handler without pump .............................43 GX-271 Preparative Liquid Handler/Injector ..................14 GX-274 Liquid Handler ...........................................................44 Training GX-271 Analytical Liquid Handler/Injector .....................15 Quad-Z 215 Liquid Handler ..................................................45 Gilson provides an extensive program of instrument and software training for users. Standard courses as GX-271 Liquid Handler/Injector without pump ............15 Quad-Z 215 Liquid Handler with Disposable Tips ........45 well sessions customized to the users specific needs are offeredMultiple at Gilsons innovative training center or 215as Liquid Handler/Injector .................................................16 Probe 215 Liquid Handler ....................................46 Multiple Probe Liquid Handler/Injector ...................16 215 Liquid Handler in the users own215 laboratory. Computer assisted training courses are also available on ..................................................................46 CD-ROM or the Gilson 223 Sample Changer ...............................................................47 on-line education center, www.training.gilson.com.
HPLC: Pumps
17 21
333/334 Prep-scale HPLC Pumps .......................................18 331/332 Semi-preparative HPLC Pumps..........................19 321/322 Analytical to Semi-preparative HPLC Pumps ..........................................................................19 30X Series HPLC Pumps .........................................................20 402 Syringe Pumps ..................................................................20 Minipuls Evolution Peristaltic Pump ...............................21 Gilson, Inc. World Headquarters Minipuls 3 Peristaltic Pump ..................................................21
Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 HPLC: Detectors
Accessories
48 52
3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA
VALVEMATE II Valve Actuator ............................................49 Orbital Shaker ............................................................................50 RackCount System ................................................................50 818 AutoMix ...............................................................................51 852/853/854 Peltier Racks .....................................................51 242/542 Peltier Racks ..............................................................52 Racks - Standard and Custom ..............................................52
References sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com

171/172 Diode Array Detectors...........................................23 15X Series UV/VIS Detectors.................................................23 Gilson S.A.S. 112 UV Detector........................................................................24 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE ANCE PREPELS II Detector .................................................................24
Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020
22 24
53 70
HPLC: Fraction Collectors
25 27
PREPFC Fraction Collector.....................................................26 FC 203B/FC 204 Fraction Collectors ..................................27
Control Software
28 30
TRILUTION LC Software..............................................................29 TRILUTION LH Software .............................................................30
GPC Clean-up Systems
31 34
Gilson Rack/Instrument Compatibility Chart ....... 53 56 Gilson Probe Selection Guide .................................... 57 60 Periodic Table ............................................................................61 Flow Cell Selection Chart.......................................................61 pH of Common Acids and Bases .........................................62 Column Sizes and Capacities ...............................................62 Maximum UV Absorbance for Commonly Used Solvents..........................................63 Optimizing Preparative HPLC ..............................................64 Separation Scale-up Equations ...........................................64 Solvent Miscibility Table ........................................................65 Solvent Polarity Chart .............................................................65 Common Conversion Tables ...................................... 66 67 Gilson Applications........................................................ 68 70
Automated GX-271 GPC Clean-up System......................32 GX-271 GPC/SPE Clean-up System.....................................33 Manual GPC Clean-up System .............................................34
Gilson HPLC Systems
Gilson high performance liquid chromatography (HPLC) systems provide complete solutions for all purication needs. Front-end liquid handlers and autosamplers are available in a range of bed capacities and optimized bench space footprints. All models accommodate innovative injection and fraction collection capabilities within a single platform. TRILUTION LC Software completely controls the entire HPLC system, allowing focus to remain on the science rather than the software.
Gilsons VALVEMATE II Valve Actuator oers multiple column selection availability featuring: fast switching times, high pressure valve control of 6 or 10-port, 2, 6 or 10-position and low pressure valve control of 6 or 8-positions.
(see page 49)
The GX-271 Liquid Handler, is a mid-size capacity and throughput instrument fullling numerous laboratory needs at an economical price.
(see pages 7, 8, 14 & 15)
The 215 Series Liquid Handlers, known as the workhorses of the industry, are available in single, multiple and variable spanning probe models.
(see page 16)
Gilsons accurate pumping solutions are available with multi-solvent selection, high pressure variable mixing and interchangeable pump heads.
(see pages 17 21)
The GX-281 Liquid Handler is Gilsons premier front-end instrument featuring a large bed capacity and a wide array of components such as automatic reinjection of fractions using innovative GX Z-Injection technology.
(see pages 5, 6, & 13)
Gilsons variety of detection solutions oers excellent sensitivity and reproducibility including diode array (DAD), ultraviolet (UV) and/or visible (VIS) and evaporative light scattering (ELS) detection.
(see pages 22 24)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Systems.
HPLC: Systems
Column ID
100 mm 150 mm 4.6 mm 10 mm 20 mm 30 mm 50 mm 2 mm
The GX-281 Preparative HPLC System allows for injection, fraction collection and reinjection. The system is also ideal for liquid handling techniques such as sample mixing, dilutions and sample extract transfers. Description Page Number
Specifications S
Sample Loading Capacity 15 g/injection Injection Loop Volume 250 L 25 mL Optimal Column Size 4.6 100 mm Flow Rate 1 200 mL/min Maximum Injection Pressure 3,000 psi (207 bar) Maximum Pump Pressure 3,040 psi (210 bar)
GX-281 Preparative HPLC System GX-281 Analytical to Semi-preparative HPLC System GX-271 Preparative HPLC System GX-271 Analytical to Semi-preparative HPLC System 845ZPREP Semi-preparative to Preparative HPLC System cLC Analytical to Preparative HPLC System
flow rate 1 200 mL/min injection loop volume 250 L 25 mL sample mass 15 g/injection flow rate 0.15 30 mL/min injection loop volume 2 L 2 mL sample mass 300 mg/injection flow rate 1 200 mL/min injection loop volume 250 L 25 mL sample mass 15 g/injection flow rate 0.15 30 mL/min injection loop volume 2 L 5 mL sample mass 300 mg/injection flow rate 1 200 mL/min injection loop volume 100 L 10 mL sample mass 15 g/injection flow rate 1 200 mL/min injection loop volume 5 L 25 mL sample mass 15 g/injection flash chromatography flow rate 25 40 mL/min injection loop volume up to 25 mL aspiration ow rate 5 mL/min for fraction loading
GX Direct Injection technology features an injection port that connects directly to the valve, minimizing volume associated with connective tubing.
(located behind the racks)
6
The prep-scale HPLC pumps feature low-dwell volumes which allow for fast, reproducible gradients.
(see pages 17 21)
The GX Z-Injection Module allows sample aspiration directly into the valve without a separate injection port, eliminating a source of potential carryover.
9
The GX Prep Solvent System features a dynamic range of volumes and ow rates using syringeless technology within a solvent delivery pump capable of accessing ve o-bed solvents.
GX-271 Fraction Trapping HPLC System
10
HPLC: Systems
The GX-281 is a multi-functional instrument that can perform injection, fraction collection and reinjection.
Specifications
Sample Loading Capacity 300 mg/injection Optimal Column Size 2 20 mm Injection Loop Volume 2 L 2 mL Flow Rate H1: 150 L/min 15 mL/min H2: 30 L/min 30 mL/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar)
A single, mid-size instrument for injection and fraction collection with separate uid pathways to reduce cross-contamination of fractions.
Specifications
Sample Loading Capacity 15 g/injection Optimal Column Size 10 100 mm Maximum Injection Pressure 3,000 psi (207 bar) Injection Loop Volume 250 L 25 mL Flow Rate 1 200 mL/min Maximum Pump Pressure 3,040 psi (210 bar)
The GX Z-Injection Module allows fast injections with low carryover and low maintenance by aspirating the sample directly into the valve.
Maximum Injection Pressure 5,000 psi (345 bar)
The GX Prep Solvent System uses syringeless technology and allows access to ve o-bed solvents. Separate injection and fraction collection paths reduce cross-contamination of fractions from sample injection. The cycle time of the injection is minimized by eliminating rinse steps which are required with single uid path congurations. Semi-preparative HPLC pumps are oered to perform binary gradients.
(see pages 17 21)
The GX-281 features a Jet Wash Rinse station design that delivers individual streams of solvent for each separate rinse station to actively wash the outside of probes. Four rinse solvents are available for selection.
The GX Direct Injection Module can utilize both 1/16" and 1/8" injection valves, allowing for a wide variety of sample volumes and loop options.
Binary gradient preparative HPLC pumps provide high-ow capabilities.

(see pages 17 21)
A VALVEMATE II Valve Actuator can be incorporated for multiple column selection or column regeneration applications.
(see page 47)
Detection solutions are available for DAD, xed or variable wavelength UV/VIS, UV and ELS detection.
(see pages 22 24)
Mid-to-large collection capacity in a small footprint.
Modular detector options available.

(see pages 22 24)
HPLC: Systems
The GX-271 is a mid-size liquid handler/fraction collector with a bench-saving footprint featuring separate fluid pathways that reduce cross-contamination of samples and fractions.
Specifications
Sample Loading Capacity 300 mg/injection Optimal Column Size 2 20 mm Maximum Injection Pressure 5,000 psi (345 bar) Injection Loop Volume 2 L 5 mL Flow Rate H1: 150 L/min 15 mL/min H2: 30 L/min 30 mL/min Maximum Pump Pressure H1: 8,700 psi (600 bar) H2: 4,350 psi (300 bar)
Gilsons 845ZPREP System features Gilsons first generation Z-Injection technology accommodating volumes and flow rates for semi-preparative to preparative applications.
Mid-to-large bed capacity, accommodating both samples and fractions.
Specifications S
Sample Loading Capacity 15 g/injection Optimal Column Size 4.6 50 mm Injection Loop Volume 100 L 10 mL Injection Variability < 1.5% Maximum Injection Pressure 3,000 psi (207 bar) Maximum Pump Pressure 3,040 psi (210 bar) Carryover < 0.01% Flow Rate 1 200 mL/min
The GX Analytical Solvent System features innovative syringeless technology and access to ve o-bed solvents.
Binary gradients are achieved with analytical to semi-preparative HPLC pumps.

(see pages 17 21)
For conrmation or added detection capabilities of nonchromaphoric compounds congure an additional ELS detector with a multi-channel DAD detector.
(see pages 22 24)
Gilsons cLC System is a complete chromatography system capable of automating the purification of compounds using both normal and reversed-phase columns. The cLC System has the ability to accommodate various modes of detection including DAD, UV/VIS, ELS and RI. Additional analytical components are also integrated onto the cLC System enabling automated on-line evaluation of the collected fractions for purity and recovery.
S Specifications
Sample Loading Capacity 15 g/injection Optimal Column Size 4 100 mm Injection Loop Volume 5 L 25 mL Flow Rate 1 200 mL/min Maximum Pump Pressure 33X Pump: 3,040 psi 32X Pump: 8,700 psi
The low mount collection valve enables fraction collection to tubes and contains two separate liquid paths which reduce cross-contamination.
Maximum Injection Pressure 5,000 psi (345 bar)
HPLC: Systems
Gilsons revolutionary Fraction Trapping System is unlike any other system on the market today. The Fraction Trapping System is ideally suited for processing fractions up to 20 mL in volume and concentrations up to 500 mg that have been purified on virtually any preparative HPLC system. Washing steps can be performed for removal of salts, buffers and mobile phase modifiers such as TFA that can hinder further LC/MS analysis. Fractions containing greater than even 50% water content can be processed and collected in 100% of organic solvent. This allows for fast dry down times at lower temperatures for samples that are heat sensitive. Dry down times of 12 24 hours can be reduced to less than 30 minutes.
Process fractions in 8 to 12 minutes with volumes up to 20 mL and concentrations up to 500 mg.
Specifications S
Steps involved in Fraction Trapping TRAP Sequestration from fraction of desired solute(s) by a solid phase RINSE Removal of buers, modiers, salts, etc. by appropriate rinsing of the solid phase and the trapped solute(s) ELUTE Trapped solute(s) desorbed to produce a relatively small volume of dry organic solvent solution of the desired solute Most reversed phase fractions contain high water content, salts, modiers and buers resulting in long dry down times up to 24 hours. Gilsons Fraction Trapping System completely removes water content, buers, modiers and salts while reducing dry down from hours to minutes.
Flow Rate 50 mL/min Injection Loop Volume up to 25 mL Wavelength 190 700 nm Aspiration Flow Rate 5 mL/min for fraction loading
Detector Variable Dual Wavelength
GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Sample Diameter Quantity 10 mm 13 mm 15 mm 16 mm 17 mm 18 mm 540 300 220 220 220 220
Optional breakthrough detector and secondary fraction collector
Dual Column with re-generation for increased throughput and solvent selection of up to eight dierent solvents.
Product #: 21120001(GX-271 Fraction Trapping System)
28 mm 70 Note: tubes up to 200 mm tall can be accommodated
Trapping Columns 10 mm ID x 150 mm 21.2 mm x 150 mm
Trapping Column Specifications Recommended Load Capacity Recommended Flow Rate 200 mg 500 mg 25 mL/min 40 mL/min
11
HPLC: Liquid Handlers/Injectors

Liquid Handler GX-281 Liquid Handler/ Injector Fraction Collection Injection Loop Volume Liquid Handling Accuracy Injection Carryover Page Number
Specifically engineered for semi-preparative to preparative HPLC requirements, the GX-281 offers unmatched versatility and performance for front-end LC automation including revolutionary syringeless pumping technology.
Specifications
Reproducibility < 1% CV (total loop) < 3.5% (partial loop) Injection Loop Volume 250 L 25 mL Flow Rate 1 200 mL/min Maximum Injection Pressure 5,000 psi (345 bar) Dimensions (w x d x h) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in)
GX-281 Liquid Handler/Injector Bed Capacity
Accuracy 98% (100 L 25 mL)
Injection Carryover < 0.005%
250 L 25 mL
98% (100 L 25 mL)
< 0.005%
13
Tube Diameter 13 mm 15 mm
Sample Quantity 576 576

Fraction Zone, 9 Fraction Zone, 10
Injection, collection and analysis
% Mobile Phase
25 mm 28 mm
180 162
GX-271 Analytical Liquid Handler/Injector
2 L 2 mL
98% (10 L 25 mL)
< 0.05%
15
Note: tubes up to 220 mm tall can be accommodated

(see page 53 for the Gilson Rack/Instrument Compatibility Chart)
mVolts
GX-271 Preparative Liquid Handler/Injector
16 mm
450 420
250 L 25 mL
98% (50 L 25 mL)
< 0.05%
14
18 mm
Time (minutes)
215 Liquid Handler/Injector
5 L 10 mL
99.7% (50 L)
< 0.1%
16
Immediate response aspiration and dispensing with the GX Prep Solvent System eliminates time spent waiting for valves to switch and syringes to ll. Two unique and separate liquid pathways for injection and fraction collection provide a zerocontamination environment. The GX Direct Injection Module features an injection port that connects directly to the injection valve for decreased dead volume and reduced maintenance.
Multiple Probe 215 Liquid Handler/Injector
5 L 5 mL
99.2% (500 L)
< 0.05%
16
Gilsons liquid handlers and injectors offer the broadest range of instrument capabilities on the market today. From small footprint injectors to larger footprint liquid handler/injector/fraction collectors, Gilson provides the ultimate front-end automation solutions for every HPLC system.
Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler with bar code) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK) 261353 (GX Z-Injection Module)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors.
Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3 Fraction Zone, 4 Fraction Zone, 5 Fraction Zone, 6
13
Specifications
GX-271 Preparative Liquid Handler/Injector
Flow Rate 1 L/min 50 mL/min Injection Loop Volume 250 L 25 mL Accuracy 98% (50 L 25 mL) Injection Carryover < 0.05% Reproducibility < 1% CV (total loop) Maximum Injection Pressure 5,000 psi (345 bar) Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)
The GX-271 Liquid Handler/Injector is the perfect blend of size, performance and capacity, consuming only 23" of linear bench space. Large-bore injection modules feature a continuous flow path design for preparative applications. Small-bore valves are available for analytical to semi-preparative applications with inert injection modules for bio-compatible applications.
The GX-271 Liquid Handler/Injector can be configured for large injection loop volumes with minimal sample waste and carryover as well as to accommodate either the GX Prep or Analytical Solvent System which feature syringeless pumping technology.
Specifications
GX-271 Analytical Liquid Handler/Injector
Flow Rate 1 L/min 5 mL/min Injection Loop Volume 2 L 2 mL Accuracy 98% (10 L 25 mL) Injection Carryover < 0.05% Reproducibility < 1% CV (total loop) < 3.5% (partial loop) Maximum Injection Pressure 5,000 psi (345 bar) Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)
Recovery data: Theophylline (5 mg/mL), Caeine (5 mg/mL) and Phenol (3 mg/mL) solution with 1 mL injection loop volumes
calculated from reinjection of collected Theophyline fractions (Zone 2)
Series of ten 20 L total loop injections of Theophylline, Caeine and Phenol Ethernet based communication provides better connectivity.
% Mobile Phase
100 80
% Mobile Phase mVolts
300 250 200

Fraction Zone, 1 Fraction Zone, 2 Fraction Zone, 3
100 80
1250 1000
Theophylline Phenol
150 100 50
mVolts
60 40 20 0
Jet Wash Rinse technology provides owing rinse for fast and ecient rinsing protocols utilizing up to two separate owing rinse solvents.
The GX Direct Injection Module increases performance by incorporating a port that connects directly to the injection valve, reducing sample waste and maintenance.
60 40 20 0
750 500 250 0 -250 0 2 4 6

Time (minutes)
Caffeine
0 -50 0 1 2
Time (minutes)
10
12
Injection 1 2 3 4 5 6 7 8 9 10 Average Recovery Standard Deviation CV
Recovery 99.86% 96.61% 98.22% 96.64% 100.10% 98.99% 99.57% 99.69% 99.81% 100.36% 99.99% 0.01 1.39% Available in an analytical or prep model, the solvent systems feature dynamic ow rate ranges and can access ve o-bed solvents.
Peak Theophylline Caffeine Phenol
Retention Time (minutes) 2.8 4.2 8.4
Area Count (Vmin/100) 14901136.11 12267689.70 13314605.43
Standard Deviation 44928.68 36503.01 73087.21
CV 0.30% 0.30% 0.55%
GX-271 Preparative and Analytical Liquid Handler/Injector Bed Capacity Tube Sample Diameter Quantity 10 mm 13 mm 15 mm 16 mm 540 300 220 220 220 220
Separate fraction collection ow paths prevent contamination from the injection process. Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK)
17 mm 18 mm
28 mm 70 Note: tubes up to 200 mm tall can be accommodated

(see page 53 for for the the Gilson th Gil Rack/Instrument Rack/I k/Inst tr ment Compatibility Chart)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Liquid Handlers/Injectors.
15
HPLC: Pumps
> 50 mm 10 mm
Flow Rate 333/334 Prep-scale HPLC Pumps 331/332 Semi-preparative HPLC Pumps 321/322 Analytical to Semi-preparative HPLC Pumps
Pressure 3,040 psi (210 bar)
Product #s: 2510121 (215 Liquid Handler) 251511 (819 Injection Module) 251551 (845Z Injection Module) 251552 (845ZPREP Injection Module)
1 200 mL/min
20 mm
1 mm
2 mm
4 mm
This versatile, large bed liquid handler accommodates more than 3,000 racks to hold virtually any sample vessel.
Customize by adding accessories and components to meet laboratory needs.
The 215 Liquid Handler/Injector, a true and trusted laboratory workhorse for many years, performs injection, fraction collection and reinjection of collected fractions within a single platform.
Column ID Page Number
18
Specifications S
Reproducibility liquid handling: 1.37% CV (50 L) injection: < 0.9% (1 mL) (total loop) Accuracy liquid handling: 99.7% (50 L) Injection Loop Volume 5 L 10 mL Injection Carryover < 0.1% Dimensions (w x d x h) 91 x 61 x 56 cm (36 x 24 x 22 in)
0.5 50 mL/min
8,700 psi (600 bar) H1: 8,700 psi (600 bar) H2: 4,300 psi (300 bar) 8,700 psi (600 bar)
19
H1: 0.15 15 mL/min H2: 0.3 30 mL/min
19
30X Series HPLC Pumps
10 L/min 200 mL/min
20
Inject onto FIA/MS, parallel HPLC and MUX systems using the optional 849 Multiple Injection Module.
The Multiple Probe 215 Liquid Handler/Injector is capable of high-throughput automation of all basic liquid handling tasks such as sample transfer, dilutions, mixing, reagent addition and plate reformatting.
402 Syringe Pumps
1 L/min 120 mL/min
110 psi (8 bar) for 0.1 10 mL syringes 40 psi (3 bar) for 25 mL syringe
20
Minipuls Evolution Peristaltic Pump
0.2 L/min 340 mL/min
42 psi (3 bar)
21
Specifications
Product #: 25101311 (Multiple Probe 215 Liquid Handler) Congure with the 0.015" ID analytical valve or the 0.040" ID preparative valve. The 849 Multiple Injection Module allows four simultaneous injections, ideal for parallel HPLC and MUX systems.
Reproducibility liquid handling: 0.57% CV (500 L) injection: < 2% CV (500 L) (partial loop) Accuracy liquid handling: 99.2% (500 L) Injection Loop Volume 5 L 5 mL Injection Carryover < 0.05% Dimensions (w x d x h) 97.8 x 61 x 55.8 cm (38.5 x 24 x 22 in)
Minipuls 3 Peristaltic Pump
standard (1, 2, 4 or 8 channels): 0.3 L/min 30 mL/min high-ow rate (2 or 4 channels): 1 220 mL/min
standard: 72 psi (5 bar) high-ow rate: 42 psi (3 bar)
21
Multiple Injection Module Option

Valve Switching Speed RV700-100: < 135 ms RV700-112: < 370 ms Dimensions (w x d x h) 61 x 18 x 13 cm (24 x 7 x 5 in)
Gilson offers unsurpassed flexibility in HPLC pumping systems with an extensive product range and accommodations.
Product #: 2515154 (849 Multiple Injection Module)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Pumps.
17
HPLC: Pumps
Capable of fast separations with high-efficiency columns in normal and reversed-phase modes, Gilsons 333 and 334 Pumps are the premier solvent delivery solutions for preparative chromatography. Choose the 333 master pump for isocratic applications or the 333/334 binary system for composition gradient with high pressure mixing (third pump for ternary).
Stackable to conserve bench space. Capable of milligram-to-gram level mass-throughput per injection up to 15 g depending on column size and load capacity. Selection of up to four solvents per pump for a total of eight solvents in a binary pumping system.
Specifications
Flow Rate 1 200 mL/min (3,040 psi) Accuracy 1% or 5.3 L/min
Gilsons 331 and 332 dual-piston pumps for semi-preparative and preparative chromatography feature a variable volume, dynamic mixer for optimized gradient performance.
TRILUTION LC Software provides control within a complete semi-preparative system including data processing and result reporting or stand-alone control via the keypad incorporated on the 331 Pump.
Smooth linear transposition from analytical to preparative HPLC methods using 4 to 20 mm ID columns packed with higheciency stationary phases. Milligram-level sample mass-throughput per injection; 150 mg with a 20 mm bore column. Solvent selection on each pump with optional 4-solvent valve for up to eight solvents.
Reproducibility < 0.5% or 2.7 L/min RSD Dimensions (w x d x h) 333: 26 x 41 x 51 cm (10 x 16 x 20 in) 334: 26 x 41 x 39 cm (10 x 16 x 15 in)
Specifications
The entire preparative HPLC system is controlled with TRILUTION LC Software, additionally the 333 model has an incorporated keypad for stand-alone operation. The ow rate can be doubled with additional parallel pumps to 400 mL/min.
Flow Rate 0.5 50 mL/min (8,700 psi) Accuracy 2% or 13 L/min
Reproducibility H2: 0.7% or 7 L/min Dimensions (w x d x h) 331: 26 x 41 x 51 cm (10 x 16 x 20 in) 332: 26 x 41 x 39 cm (10 x 16 x 15 in)
Product #s: 38103312 (right) (331 Pump, H2 primary solvent, dual-piston, reciprocating master pump) 38103322 (left) (332 Pump, H2 secondary solvent, dual-piston, reciprocating remote controlled pump)
Gradient Linearity and Accuracy
Programmed Composition (% E) 100 80 60 40 20 0 0 5 10 15 20 Time (minutes) 25 30 250 200 150 100 50 0 Detected Composition (mAU) 120 300
The 321 and 322 multi-solvent pumps are designed for use with Gilsons HPLC analytical and semi-preparative chromatography systems.
High pressure Adjustable Volume Dynamic Mixer (AVDM) enables fast gradient response times and homogeneous mixtures for analytical to semi-preparative applications. While the pumps are controlled via TRILUTION LC, the 321 also incorporates a keypad that allows independent stand-alone gradient control.
Retention Time Repeatability

210 180 150 120 90 60 30 0 -30 100 80 60 40 20 0 0 2 4 Time (minutes) 6 8 % Mobile Phase
A single 321 or 322 Pump is capable of binary gradients.
mVolts
H2 pump heads are ideal for 4 to 20 mm ID columns.
Specifications
Flow Rate H1: 0.15 15 mL/min (8,700 psi) H2: 0.3 30 mL/min (4,300 psi) Accuracy 2% or 13 L/min
Series of Ten Injections Methyl PB Average Retention Time Standard Deviation CV 3.44 0.02 0.72% Ethyl PB 4.85 0.02 0.34% Propyl PB 6.08 0.02 0.29%
Reproducibility H1: < 0.7% or 2 L/min H2: 4 L/min
Product #s: 38103331 (left) (333 Pump, H3 primary solvent, dual-piston, reciprocating master pump) 38103341 (right) (334 Pump, H3 secondary solvent, dual-piston, reciprocating remote controlled pump)
Product #s: 38103211 (321 Pump, H1) 381032211 (left) (322 Pump, H1) 38103212 (right) (321 Pump, H2) 381032221 (322 Pump, H2)
H1 pump heads are ideal for 2 to 10 mm ID columns.
Dimensions (w x d x h) 321: 26 x 41 x 51 cm (10 x 16 x 20 in) 322: 26 x 41 x 39 cm (10 x 16 x 15 in)
19
HPLC: Pumps
Together with Gilsons manometric modules and dynamic mixers, the 305, 306 and 307 Pumps offer a cost-effective, space saving and robust solution for isocratic or gradients of analytical to preparative HPLC.
The 307 model functions as an isocratic pump with a built-in manometric module. Dynamic mixers ensure ecient, dependable gradient creation.
The 306 model functions as a slave unit, controlled by either a 305 or TRILUTION LC Software.
Tools are not required for pump head removal, allowing accessibility for maintenance. Piston seals, check valves and other parts can be replaced in a matter of minutes.
Offering unmatched flexibility and performance for liquid transfer applications, the Minipuls Evolution is designed for scientists seeking a high performance, manageable peristaltic pump capable of a variety of configurations. Gilson offers the only peristaltic pump that allows up to 16 separate channel cartridges to be changed while the pump is running with a simple push on the cartridge for placement and removal.
The 305 and 307 Pumps are programmable through the incorporated keypad.
Specifications S
Reproducibility 0.1% on all channels Tubing ID standard: 0.25 4 mm high-ow: 2 8 mm
Specifications S
Flow Rate 305/306: 10 L/min 200 mL/min 307: 10 L/min 10 mL/min Reproducibility < 1% Dimensions (w x d x h) 33 x 33 x 15 cm (13 x 13 x 6 in) Accuracy < 1%
Optimized six roller drive limits pulsation.
Flow Rate standard: 0.2 L/min 70 mL/min (42 psi, 3 bar) high-ow: 1 340 mL/min (29 psi, 2 bar) Head Speed continuous adjustment from 0 60 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Dimensions (w x d x h) Module: 164 x 182 x 169 mm MF1 pump head: 164 x 56 x 169 mm MF4 pump head: 164 x 92 x 169 mm HF2 pump head: 164 x 92 x 169 mm
Product #s: 360261 (305 Pump) 3602661 (right) (306 Pump) 3602671 (left) (307 Pump)
Product #: F110701 (Minipuls Evolution Pump)
The 402 Syringe Pumps assure accuracy in sample transfers, dilutions, reagent additions, mixing and more. They offer speed and reliability for repetitive liquid handling tasks.
Available in three configurations:
The Gilson Minipuls 3 multi-channel peristaltic pump is simple to integrate onto existing systems with control through an incorporated keypad or remotely with a separate device.
The Minipuls 3 accommodates wide ow rate ranges using either the standard or high-ow pump head. Pump heads are available with one, two, four or eight channels. Multiple stainless steel rollers drive the pump to produce smoother, low pulse ows.
402 Single, ideal for sampling injectors.
402 Dual with Tee, provides rapid dilutions in both small and large volumes.
402 Dual, two-in-one accommodates simultaneous dispensing of multiple reagents and solvents.
An ideal addition to Gilson liquid handlers and injectors including the GX and 215 Series instruments.
Enhanced reliability and ease of operation are possible because the pumps speed stability is maintained independent of normal temperature variations from 0 to 40C. This makes the pump ideal for biological perfusion applications.
(Note: not intended for direct connection to human subjects.)
Specifications
Flow Rate standard: 0.3 L/min 30 mL/min (72 psi, 5 bar) high-ow: 1 220 mL/min (42 psi, 3 bar) Tubing ID standard: 0.25 4 mm high-ow: 2 8 mm Head Speed continuous adjustment from 0 48 rpm by 0.01 rpm increments up to 9.99 rpm, 0.1 rpm increments above 10 rpm Dimensions (w x d x h) 15 x 17.5 x 18 cm (5.9 x 6.9 x 7.1 in)
Specifications
Reproducibility 0.8% (10 L) Flow Rate 1 L/min 120 mL/min Dimensions (w x d x h) 17 x 20 x 24 cm (6.7 x 7.9 x 9.4 in)
Product #s: F410511 (left) (402 Single Syringe Pump) F410512 (right) (402 Dual with Tee Syringe Pump) F410513 (402 Dual Syringe Pump)
Accuracy 98.2% (10 L)
Product #: F155004 (Minipuls 3 Pump, single channel)
21
HPLC: Detectors
Evaporative Diode Array Ultraviolet Visible Spectral Page Light Scattering Wavelength Channels (DAD) (UV) (VIS) Band Width Number (ELS) 171/172 Diode Array Detectors 15X Series UV/VIS Detectors 190 720 or 190 610 nm
Gilsons 171 and 172 Diode Array Detectors deliver rugged and accurate detection for both preparative and analytical HPLC. Detection is done with a fiber optic light path that can be configured in a fixed or remote flow cell design.
With a remote ow cell, the power of the detector is located directly at the outlet of the column, which yields virtually no peak broadening. An interchangeable ow cell design allows for both the 171 and 172 Detectors to accommodate analytical and semi-preparative to preparative applications.
Specifications
S/N Ratio Pixels 171: < 3 x 10-04 AU 171: 256 172: 512 172: < 2 x 10-04 AU Pixel Distance Wavelength 171: ~2.2 nm/pixel 171: 190 720 nm 172: ~0.8 nm/pixel 172: 190 610 nm Wavelength Accuracy Wavelength Repeatability < 0.5 nm < 0.1 nm Baseline Drift Wavelength Temperature Drift 5 x 10-04 AU/h < 0.005 nm/C Dimensions (w x d x h) 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)
23
190 700 nm
9 nm
dual & single
23
Product #s: 18100001 (171 Diode Array Detector) 18100002 (172 Diode Array Detector)
112 UV Detector
254 or 280 nm
0.2 or 12 nm
single
24
PREPELS II
Detector
single
24
Gilsons 151, 152, 155 and 156 UV/VIS Detectors deliver versatile, rugged detection with an interchangeable flow cell design to accommodate analytical to preparative HPLC applications.
(see page 61 for Flow Cell Selection Chart)
The following modes are available to specify detection conditions and basic operating parameters: Single wavelength with dual sensitivities (151 and 152) Dual wavelengths (155 and 156) Scan wavelengths (155 and 156) Choose either Gilsons TRILUTION LC Software or the standalone keypad control on the 151 and 155 Detectors. Up to ten methods can be stored.
Gilson offers a complete line of HPLC detection solutions that are ideal for analytical, semi-preparative and preparative HPLC applications.
120 100 80 mVolts 60 40 20 0 -20 0 2 4 Time (minutes) 6 20 0
Product #s: 10105311 (right) (151 UV/VIS Detector, with ow cell/accessory kit) 10105411 (152 UV/VIS Detector, with ow cell/accessory kit) 10105511 (155 UV/VIS Detector, with ow cell/accessory kit) 10105611 (left) (156 UV/VIS Detector, with ow cell/accessory kit)
100 80 60 40 % Moblie Phase
Specifications
Lamp UV: deuterium, 750 hrs VIS: tungsten/halogen, 500 hrs Wavelength 190 700 nm Drift 3 x 10 4 AU/hr Sensitivity 0.001 2 AU Setting Accuracy 2 nm Setting Precision 0.2 nm
Spectral Band Width Dimensions (w x d x h) 9 nm 26.5 x 43.5 x 15.6 cm (10.4 x 17.1 x 6.2 in)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Detectors.
23
HPLC: Detectors
The 112 UV Detector is a rugged, economical detector for analytical, preparative HPLC and GPC clean-up.
A front panel dial allows selection of sensitivity level to adjust the absorbency of a sample.
Accessible lamp/lter assemblies for selecting wavelengths. Fixed wavelength design for increased sensitivity and durability. 10 mm pathlength analytical ow cell provides excellent sensitivity ranging from 0.001 to 1 AUFS (Absorbance Units, Full Scale). Reduced operating and setup time with a single step baseline function. Event-marking function conveniently marks injection point or fraction collector advance on either the recorder or data system.
PREPFC Fraction Collector

Capacity Large Small Flow Rate Microplate Tubes 1 L Bottles Page Number
200 mL/min
26
Specifications S
Lamp mercury (standard): 254 nm, 2,000 hrs phosphor-coated mercury: 280 nm, 500 hrs Spectral Band Width 254 nm: 0.2 280 nm: 12 Drift 3 x 10 4 AU/hr Wavelength 254 or 280 nm Flow Cell Sensitivity < 4 x 10 4 AU for ow step of 0.5 2 mL/min (Methanol) Dimensions (w x d x h) 32 x 32 x 13 cm (12.6 x 12.6 x 5.2 in)
FC 204 Fraction Collector
20 or 200 mL/min
27
Product #: 0310511 (112 UV Detector, with lamp and 11 L ow cell/accessory kit)
FC 203B Fraction Collector
20 mL/min
27
Gilsons evaporative light scattering (ELS) detector, PREPELS II, provides capabilities on the analytical-scale with the ability to detect as low as nanograms of analyte while a unique filtering technology removes baseline noise without distressing peak height or shape.
150 125 100 mVolts 75 50 25 0 0 2 Time (minutes) 4 6 40 20 0 100 ELSD 80 UV 60
The PREPELS II Detector is ideal for obtaining the purest fractions with the ability to detect virtually any analytes with or without a chromophore, including compounds that have poor UV absorption such as sugars, carbohydrates, lipids and triglycerides.
Gilson offers advanced solutions for fraction collection with a complete line of stand-alone and software-controlled fraction collectors ranging from small footprint to large vessel capacity models and everything in between.
Specifications S
Light Source 670 nm laser diode, < 5 mW Gas Requirement 65 5 psi Nitrogen or Argon Nebulization Temperature 0 70C Evaporative Zone Temperature ambient 120C Liquid Flow Rate 0.2 5 mL/min
Splitting packages available to accommodate system ow rates up to 75 mL/min
% Moblie Phase
Product #s: 18100100 (PREPELS II Detector, 120V) 18100200 (PREPELS II Detector, 240V)
Dimensions 25 x 46 x 29 cm (9.8 x 18 x 11.5 in)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors.
25
Gilsons PREPFC Fraction Collector is a large capacity, compact footprint fraction collector ideal for semi-preparative to preparative HPLC applications. The PREPFC features an adjustable tray that accommodates various vessel heights, volumes and sizes.
Specifications S
Dimensions (w x d x h) 50.3 x 39.1 x 34.8 cm (19.8 x 15.4 x 13.7 in) Liquid Contact Material PTFE and 316 stainless steel Valve Dead Volume 114 L internal volume; 6 L dead volume from common port to normally closed port Flow Rate 200 mL/min Collection Modes slope, level, time or volume
Choose the compact FC 203B Fraction Collector for small capacity collection or the FC 204 for larger volumes. Collect fractions by time, drop or peak, accommodating all LC applications. Both models accept a wide variety of collection vessels including tubes, vials and microplates.
Specifications S
Dimensions (w x d x h) FC 203B: 32.4 x 29.2 x 26.7 cm (12.8 x 11.5 x 10.5 in) FC 204: 47.9 x 46.4 x 33 cm (18.9 x 18.3 x 13 in) Liquid Contact Material PTFE and 316 stainless steel Valve Switching Speed 100 ms Max Flow Rate FC 203B: 20 mL/min FC 204: 20 or 200 mL/min
PREPFC Bed Capacity
Sample Quantity 320 192 176 80 54 54 18 11 8 240 56 432

mVolts
Size 13 x 100 mm (9 mL) 16 x 100 mm (12 mL) 18 x 150 mm (25 mL) 25 x 200 mm (70 mL) 28 x 57 mm (25 mL) 30 x 115 mm (50 mL) 48 x 113 mm (125 mL) (500 mL) (1 L) 12 x 32 mm (2 mL)
300.0 250.0 200.0 150v 100.0 50.0 0.0
Vessel tubes tubes tubes tubes scintillation vials conical bottom tubes bottles bottles bottle vials scintillation vials tubes tubes tubes
Rack (4) Code 343 Racks (2) Code 223 Racks (4) Code 22 Racks (2) Code 239 Racks (2) Code 204 Racks (2) Code 222 Racks (2) Code 211 Racks (1) Code 91 Rack (1) Code 92 Rack (4) Code 330 Racks (4) Code 333 Racks (4) Code 341 Racks (4) Code 343 Racks (4) Code 345 Racks
Valve Switching Speed < 20 ms
Valve Dead Volume FC 203B/FC 204: 64 L internal volume; 3.5 L dead volume from common port to normally closed port FC 204: 114 L internal volume; 6 L dead volume from common port to normally closed port
PREPFC Control Software: Remote start and tube advance Up to ten programmable collection windows Active chromatogram display with start and stop fraction collection lines Customizable collection patterns and rack addition
The 3-way diverter valve prevents contamination of collected fractions (standard on FC 204 and optional on FC 203B).
In peak mode, collectors monitor a detector signal and identify peaks using either an adaptive-slope algorithm or a specied mV threshold. The fraction collector utilizes multi-column adapters for collecting up to 18 separate channels.
Increased X/Y speed optimizes fraction collection.
High-ow fraction collection valve.
(20 mL) 10 x 75 mm (4 mL) 13 x 300 mm (9 mL)

Minutes
Up to ten programmable time windows can be added for collecting only samples of interest, while discarding the columns void volume, areas of non-interest and equilibration volumes.
Its compact size ts in most fume hoods.
320 176
-50.0
1 6 x 150 mm (20 mL) 0.0 1.0 2.0
Contact closure inputs are available to remotely start, advance or stop fraction collection.
Pocket PC control for stand-alone operation allows the PREPFC to be added to any HPLC system capable of sending contact closures.
Increase bed capacity by conguring up to four fraction collectors in a single system (provides over 1,000 fractions). Product #s: 171011 (right) (FC 203B) 171011DV (FC 203B, 3-way valve) 171041 (left) (FC 204) 171043 (FC 204, prep valve) Stand-alone instruments are controlled with an incorporated keypad or through Gilsons TRILUTION LC Software.
Product #s: 17110101 (PREPFC Fraction Collector - Ethernet) 171071 (PREPFC Fraction Collector)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson HPLC Fraction Collectors.
27
Control Software
HPLC Systems SPE Systems GPC Clean-up Systems Liquid Handlers Page Number
Gilson has redefined purification with the HPLC system control software, TRILUTION LC. This liquid chromatography software, specifically designed for purification, allows for complete control to achieve the challenging results that applications require.
TRILUTION LC
29
TRILUTION LH
30
Configuration and Virtual Lab Screen
Gilson offers powerful software to complement robust instruments. With accommodating features, drag-and-drop functionality and numerous supplied tasks, Gilsons software packages allow effortless yet complete control of instruments focusing on the science rather than the software.
Intel, Pentium and Core are trademarks of Intel Corporation or its subsidiaries in the United States and other countries. Microsoft and Windows are registered trademarks of the Microsoft Corporation in the United States and/or other countries.
Method Builder Control Screen
Fraction Collection Settings Page
Application A li i Run R Screen S Fraction Collection Capabilities: Collection modes: slope, level, time or volume Conditional logic fraction collection Graphical fraction collection optimization Slope collection settings: front, tail and APEX Auto sample list generation Open Access Module: Administrator control and permission-setting capabilities Walk-up user access Minimal training required Conforms to the application needs rather than compromising the application in order to t the software.
Product #s: 210631R21 (TRILUTION LC Media) 21063130 (TRILUTION LC Lifetime License) 21063130L5 (TRILUTION LC Multi-user Lifetime License - 5) 21063130L10 (TRILUTION LC Multi-user Lifetime License - 10) 21063131 (TRILUTION LC 1 year License) Note: Requires Oracle Database 10g Express Edition
Computer Recommendation
Processor Intel Pentium 4 (2.8 GHz); Intel Core 2 Duo (1.86 GHz) RAM 2 GB Fixed Drive 120 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color I/O Ports 1-Serial port 1-Ethernet port
Operating System Microsoft Windows XP Professional SP2 or SP3
Visit www.gilson.com or call 608-836-1551 for more details on Gilson Control Software.
29
Control Software
TRILUTION LH Software, designed by scientists for scientists, is Gilsons complete control solution for liquid handling and solid phase extraction applications. TRILUTION LH features a user-friendly graphical interface with icon-based drag-and-drop functionality minimizing the learning curve and optimizing application performance. Fraction Collection
Product #s: 210630R20 (TRILUTION LH Media) 21063020 (TRILUTION LH Lifetime License) 21063021 (TRILUTION LH 1 Year License) Note: Requires Oracle Database 10g Express Edition

Multiple Fractions Solid Phase Extraction Control Page Number Automated GX-271 GPC Clean-up System
0.25 450 mL
(optional)
TRILUTION LC Software
32
Liquid Disposable Solid Phase Handling Tips Extraction
Solvent System
Tweaks
Auxiliary
GX-271 GPC/SPE Clean-up System
0.25 450 mL
(optional)
TRILUTION Software
33
Main Menu
Manual GPC Clean-up System
0.25 < 1000 mL
(optional)
307 Pump keypad
34
Method in Method Builder Screen
SS Dispense Task Property Page
Simulation Mode in Application Run Screen
Over 70 Gilson-supplied tasks enable quick and ecient method programming. Quick and eortless instrument setup, bed layout and method creation. Sample list with spreadsheet functionality and graphical run simulation.
Run methods in either batch or sequential mode to optimize performance. Manual control for performing routine tasks such as priming instruments and troubleshooting. Graphical custom task creation and cartridge monitoring.
Computer Recommendation C
Processor Intel Pentium 4 (2.8 GHz) RAM 1 GB Fixed Drive 80 GB, available space: 4 GB (6 GB at install) Additional Drive DVD/CD-RW Video Card 1280 x 1024 with 24 bit True Color Serial Ports 2
Gilsons gel permeation chromatography (GPC) clean-up systems are an ideal solution for postextraction clean-up of interfering substances from environmental samples, food products and animal tissue, prior to analysis by GC, GC/MS, HPLC or LC/MS. Failure to remove these interfering compounds can cause poor analytical results, shorten column life and increase analytical downtime.
Gilson GPC Clean-up Systems meet clean-up guidelines set forth by the USEPA, USFDA, USDA, USGS, ISO, CFIA, AOAC International and European Union (EN 1528, EN 12393, S19) requirements.
Operating System Microsoft Windows XP Professional SP2 or later Microsoft Windows 2000 SP4
Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems.
31
The Automated GX-271 GPC Clean-up System from Gilson is capable of up to 80 sample extractions with large fraction collection capacity into an assortment of vessels.
Gilsons newly released Automated GX-271 GPC/SPE Clean-up System provides SPE capabilities in addition to GPC for sample extract purification; a multi-purpose sample preparation system.
Automated GPC Systems Bed Capacity Sample Quantity 36 85 13 8 20 8 50 32 23 12 8 Sample Quantity 80 44 Description 38 x 200 mm (170 mL) collection tubes 25 x 200 mm (70 mL) collection tubes 61 x 137 mm (250 mL) Boston Round bottles Labconco RapidVap (450 mL) at-bottom evaporation asks Caliper TurboVap II (200 mL) evaporation tubes Labconco RapidVap (450 mL) evaporation asks, with stem Caliper TurboVap II (50 mL) evaporation tubes Labconco RapidVap (100 mL) at-bottom evaporation asks 49 x 114 mm (125 mL) Boston Round bottles Pyrex 4100 (125 mL) at-bottom asks for rotary evaporators Pyrex (250 mL) at-bottom asks for rotary evaporators Description 13 x 100 mm (8 mL) tubes 16 x 100 mm (12 mL) tubes GPC Collection Rack Code 320 Rack Code 321 Rack Code 322 Rack Code 323 Rack Code 324 Rack Code 325 Rack Code 326 Rack Code 327 Rack Code 328 Rack Code 329 Rack Code 331 Rack GPC Sample Rack Code 343 Rack Code 346 Rack Product # 260440050 260440051 260440052 260440053 260440054 260440055 260440056 260440057 260440058 260440059 260440060 Product # 260440025 260440049
Specifications
Automated GPC Systems
Flow Rate 0.01 10 mL/min Injection Loop Volume 20 L 5 mL Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar)
Compatible with various GPC columns from low pressure glass to high pressure stainless steel.
Mobile Rack technology enables programmed switching from drain and collect positions to completely automate the entire SPE process from condition to load, wash and elute.
The GX Direct Injection Module reduces sample waste and carryover, which prevents the need to overll the sample loop.
Sample Capacity SPE: 60 (3 mL), 45 (6 mL) GPC: 80
Positive Pressure elution increases reproducibility and precision.
Product #s: 21110000 (Automated GX-271 GPC Clean-up System, with 112 UV Detector) 21110001 (Automated GX-271 GPC Clean-up System)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson GPC Clean-up Systems.
33
Gilsons Manual GPC Clean-up System is a simple and economical solution within a small footprint requiring minimal bench space.
Specifications
Flow Rate 0.01 10 mL/min Maximum Fractions 10 separate Wavelength 254 or 280 nm Maximum Pressure 4,000 psi (276 bar)
Solid Phase Extraction Systems

Cartridge Sizes Liquid Handler GX-271 ASPEC Multiple Collection Positive Pressure Elution 1 mL 3 mL 6 mL Microplate Page Number
Injection Loop Volume 20 L 5 mL
36
GX-274 ASPEC
37
Quad-Z 215 Liquid Handler for SPE

Manual injection valve coordinates injection time. Collection of multiple fractions from the same sample extract is done with the VALVEMATE II Valve Actuator.
38
SPE 215 System
39
Integrate a 112 UV Detector for EPA method compatibility.
Code 300 Series Racks
39
Keypad provides simple setup and control of the system for time-based fraction collection.
Product #s: 21110002 (Manual GPC Clean-up System, with 112 UV Detector) 21110003 (Manual GPC Clean-up System)
Automate solid phase extraction (SPE) processes with Gilsons complete instrument line. From 96-well extraction plates to 1, 3 or 6 mL cartridges, Gilson automated SPE solutions meet any application need.
Conditioning
Loading Sample
Washing
Eluting
Analyte
Interferences
Visit www.gilson.com or call 608-836-1551 for more details on Gilson SPE Systems.
35
The GX ASPEC Series provides rugged and reliable automation of SPE applications utilizing 1, 3 or 6 mL cartridges. The GX-271 ASPEC is truly the solid solution for low to mid-throughput sample clean-up and extraction.
Specifications
GX-27X ASPEC Series
Cartridge Capacity 108 (1 mL), 60 (3 mL), 45 (6 mL) Accuracy 98% (100 L 25 mL) Positive Pressure Modes syringe or gas cylinder/source Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)
The GX-274 ASPEC is an ideal solution for mid to high-throughput sample clean-up and extraction.
Process four SPE cartridges simultaneously for increased throughput. Mobile Rack technology enables the entire SPE process to be automated from the initial condition to the nal elution and transfer while allowing access to cartridges and compounds of interest. The linear rail system permits automated method development and method optimization in which all or selected steps are collected and evaluated.
A utomated S olid P hase E xtraction C artridges
Utilizes Mobile Rack technology to completely automate the SPE process by increasing capacity and exibility while performing both SPE and liquid handling.
Optional safety shield available.
Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision in comparison to vacuum based instruments.
On-bed transfer ports allow access to two additional large volume solvent reservoirs; incorporating a VALVEMATE II provides access to ten o-bed solvents.
On-bed access to four 500 mL or 700 mL reservoirs.
Built-in pressure sensing on each uid line monitors cartridge pressure and responds to blockage by activating an error handling method.
Positive Pressure elution increases reproducibility and precision due to process consistency.
Choose between the 406 Single or, for more exibility, the 406 Dual Syringe Pump for both large and small volume capabilities. Both pumps have built-in pressure sensing capabilities for detecting clogged uid lines and monitoring cartridge pressure on each uid line.
Integrate a GX Direct Injection Module for on-line injection onto HPLC or LC/MS following SPE clean-up.
Product #s: 2614007 (left) (GX-271 ASPEC, with 406 Single Syringe Pump) 2614008 (right) (GX-271 ASPEC, with 406 Dual Syringe Pump) 261355 (GX Direct Injection Module, 1/8", prep) 261354 (GX Direct Injection Module, 1/16", prep) 261356 (GX Direct Injection Module,1/16", analytical SS) 261357 (GX Direct Injection Module, 1/16", analytical PEEK)
Product #: 2614010 (GX-274 ASPEC, with two 406 Dual Syringe Pumps)
37
Gilsons Quad-Z 215 Liquid Handler is a flexible and robust system that performs liquid handling tasks, on-bed filtration and Positive Pressure SPE. This liquid handler accommodates various racks maximizing flexibility for high-throughput applications.
Specifications
Cartridge Capacity 288 (1 mL), 144 (3 mL), 60 (6 mL) Accuracy 98% (100 L 25 mL)
Dimensions (w x d x h) 91 x 61 x 61 cm (36 x 24 x 24 in)
The SPE 215 System provides an automated solution for sample preparation, sample reconstitution and parallel injection onto HPLC or LC/MS systems. Additionally, the SPE 215 enables parallel clean-up, filtration and purification of combinatorial libraries and biological samples.
Positive Pressure elution is achieved with Gilsons integrated Sealing Foot design.
Mobile Racks automate entire SPE and liquid handling processes.
Four independent probes with independent variable spacing ranging from 9 to 18 mm for access to various vessel sizes.
Using Code 300 Series Racks with Sealing Cap technology enables Positive Pressure SPE, ensuring better reproducibility and higher precision.
(see Code 300 Series Rack Capacity chart on page 39)
The SPE 215 accommodates various racks oering sample preparation solutions for virtually any application.
Up to eight samples can be processed every ve minutes or 96 samples in an hour; a solution ideal for high-throughput sample clean-up applications.
Specifications
Reproducibility 0.98% (100 L) Accuracy 97.1% (100 L)
Dimensions (w x d x h) 97.8 x 61 x 64 cm (38.5 x 24 x 25.2 in)
Product #: 25101322 (SPE 215 System)
Gilsons Code 300 Series Racks provide the ability to add SPE and filtration to Gilson liquid handlers including the 215 and GX Series instruments.
Mobile Rack technology enables automated switching from drain and collect positions to accomplish the entire SPE process from condition to load, wash and elute, including selective evaluation of individual steps for method development and optimization available through N+1 movement into individual collection tubes. Rack Product # 2504300 2504301 2504302 2504303 2504304 2504305 2504306 2504307 Sealing Cap technology enables Positive Pressure SPE ensuring better reproducibility and higher precision.
Code 300 Series Rack Capacity
Sample SPE Quantity Cartridge 96 96 48 48 48 32 20 20 Product #: 25101413 (Quad-Z 215 Liquid Handler for SPE ) 1 mL tabless
Collection Vessel 96-well microplate, deep
Code 300 Rack
1 mL tabless 96-well microplate, shallow Code 301 Rack 1 mL 3 mL 3 mL 3 mL 6 mL 6 mL 12 x 32 mm (2 mL) vials 12 x 32 mm (2 mL) vials 12 x 75 mm (5 mL) tubes 15 x 45 mm (4 mL) vials 12 x 75 mm (5 mL) tubes 15 x 45 mm (4 mL) vials Code 302 Rack Code 303 Rack Code 304 Rack Code 305 Rack Code 306 Rack Code 307 Rack
39
Liquid Handlers
Multiple Probes GX-281 Liquid Handler GX-271 Liquid Handler Disposable Tips Reproducibility Accuracy Page Number < 1% (100 L +) 98% (100 L 25 mL) 41
The GX-281 Liquid Handler is Gilsons premier liquid handling instrument. The GX-281 offers unmatched versatility and performance for liquid handling operations including tube-to-plate transfers, serial dilutions, compound reconstitution and sample filtering through the use of Code 300 Series Racks and more. The efficient speed of the GX-281 allows applications to be completed quickly and reliably. The GX Solvent Systems offer a wide range of volumes and flow rates via patented syringeless technology.
Access ve o-bed solvent reservoirs for bulk reagent dispensing and multiple solvent rinsing protocols.
Specifications
Reproducibility < 1% (100 L +) Dimensions (w x d x h) 87.5 x 76.2 x 96.5 cm (35 x 30 x 38 in) Accuracy 98% (100 L 25 mL) Flow Rate 1 L/min 50 mL/min
25 Volume Delivered 20 15 10 5 0 0 2 4 25 mL Transfers at 25 mL/min. 10 mL Transfers at 25 mL/min. 6 8 10 12 Transfers
< 1% (100 L 25 mL)
98% (50 L 25 mL) 98% (10 L 5 mL)
42
Aliquot GX-274 Liquid Handler Quad-Z 215 Liquid Handler Quad-Z 215 Liquid Handler with Disposable Tips Multiple Probe 215 Liquid Handler 215 Liquid Handler 0.8% (10 L) 98.2% (10 L) 44
Direct request aspirating and dispensing eliminates the time spent waiting for syringes to ll.
Dilution
Mixing
10 Measured Volume 7.5 5 2.5 0
Optional integrated bar code scanner veries rack types and positions.
5 L Dispense
2.1% (1 L of DMSO)
104% (1 L of DMSO)
45
20
40
60
80 100 120 140 160 180 Dispenses
4.3% (1 L of DMSO)
113% (1 L of DMSO)
45
0.57% (500 L)
99.2% (500 L)
46
GX-281 Liquid Handler Bed Capacity
1.37% (50 L of DMSO)
99.7% (50 L of DMSO)
46
Tube Diameter 13 mm
Sample Quantity 576 576 450 420 180 162
223 Sample Changer
0.8% (10 L)
98.2% (10 L)
47
15 mm 16 mm 18 mm 25 mm
Gilson liquid handlers are industry renowned for rugged, reliable performance at an economical price. Whether the application requires a single probe, multiple probes, variable spacing or disposable tips, Gilsons liquid handling solutions meet all needs.
28 mm
Note: tubes up to 220 mm can be accommodated Product #s: 261030 (GX-281 Liquid Handler) 261031 (GX-281 Liquid Handler, with bar code scanner)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson Liquid Handlers.
41
Liquid Handlers
Specifications
GX-271 Preparative Liquid Handler
Accuracy 98% (50 L 25 mL) Flow Rate 1 L 50 mL/min
Specifications
GX-271 Analytical Liquid Handler
Accuracy 98% (10 L 5 mL) Flow Rate 1 L 5 mL/min
Dimensions (w x d x h) 59.7 x 54.1 x 57.1 cm (23.5 x 21.3 x 22.5 in)
GX Prep Solvent System Transfers

50 L 100 L 500 L 1 mL 5 mL 10 mL 25 mL
% CV
1.4 1.2 1 0.8 0.6 0.4 0.2 0
The GX-271 Liquid Handler oers a medium capacity with a small footprint. The GX-271 models are economical and robust liquid handlers for reformatting, reagent dispensing and performing serial dilutions. Their modular design allows for future expansion by adding injection and fraction collection capabilities.
(see GX-271 Liquid Handler/Injector page 14)
Consumes only 23" of linear bench space. Flexible bed accommodates ve racks and virtually any tube, vial or microplate. Ethernet based communication provides better connectivity.
% CV
GX Analytical Solvent System Transfers

1 L 14 12 10 8 6 4 2 0 10 L 1 mL 5 mL
Volume
Volume
GX Prep Solvent System Transfers

Volume 50 L 100 L 500 L 1 mL 5 mL 10 mL 25 mL CV 1.25% 1.18% 0.48% 0.14% 0.09% 0.07% 0.02% Accuracy 2 L 4 L 7 L 5 L 16 L 105 L 46 L
Liquid Level Detection allows for detecting a variety of solvents as low as 20 L in a 384-well microplate.
GX Analytical Solvent System Transfers

Volume 1 L 10 L 1 mL 5 mL CV 11.98% 4.5% 0.18% 0.2% Accuracy 300 nL 1 L 20 L 100 L
GX-271 Preparative and Analytical Liquid Handler Bed Capacity Tube Diameter 10 mm 13 mm 15 mm Sample Quantity 540 300 220 220 220 220 70
Jet Wash Rinse technology provides owing rinse for fast and ecient rinsing protocols utilizing up to two separate owing rinse solvents.
16 mm The GX-271 Liquid Handler is available without a pump or GX Solvent System, allowing the instrument to be congured with a 402 Syringe Pump. 17 mm 18 mm 28 mm
Gilsons GX Solvent System, available in an analytical or prep model, features a dynamic range for volumes and ow rates and can access ve o-bed solvents.
Note: tubes up to 200 mm can be accommodated

Product #s: 2614107 (GX-271 Preparative Liquid Handler) 2614108 (GX-271 Analytical Liquid Handler) 2614101 (GX-271 Liquid Handler without pump)
43
Liquid Handlers
The GX-274 Liquid Handler is a mid-throughput, four probe instrument capable of processing up to four samples (at 18 mm fixed spacing) in parallel, increasing the efficiency of liquid handling applications. The GX-274 Liquid Handler provides an economical option with a small footprint for achieving increased throughput in a variety of applications. The GX-274 is ideal for bulk reagent dispensing when used in conjunction with a Minipuls Evolution or Minipuls 3 Peristaltic Pump or for tube-to-plate transfers using 402 Syringe Pumps.
Specifications
Accuracy 98.2% (10 L) Reproducibility < 0.8% (10 L +)
The Quad-Z 215 Liquid Handler was designed based on the robust Gilson 215 Liquid Handler. This instrument accommodates numerous racks for maximum flexibility of sample transfer between tubes, vials and microplates.
Specifications
Dimensions (w x d x h) 91 x 61 x 61 cm (36 x 24 x 24 in) Accuracy 104% (1 L of DMSO) Reproducibility liquid handling: 2.1% (1 L of DMSO) injection: < 2.0% (500 L syringe, partial loop); < 5.0% (500 L syringe, total loop) Injection Loop Volume 5 L 5 mL
Access four on-bed solvent reservoirs without consuming a rack position.
Flexible rack system accommodates tubes, vials and microplates. Optional owing rinse station with solvent ports provides active probe rinsing from o-bed solvents. A VALVEMATE II can be incorporated enabling access to ten additional o-bed solvents.
Gilsons Quad-Z 215 Liquid Handler can be configured with a Disposable Tips option, a requirement in molecular biology and biochemistry applications. Disposable tips prevent crosscontamination, which saves time by eliminating rinsing steps. This system includes on-bed storage of tips, as well as a chute for collection of used tips.
Flexible conguration enables use of xed probes and 10 L, 200 L and 1 mL disposable tips.
Injection Carryover < 0.05%
Disposable Tips Option

Accuracy 113% (1 L of DMSO) Reproducibility 0.8% (10 L +); 4.3% (1 L of DMSO)
Integrating two 402 Dual Syringe Pumps provides four independently controlled syringes from 100 L to 25 mL.
For access to the various vessel sizes, the four independent probe variable spacing ranges from 9 to 18 mm.
Product #s: 2614102 (GX-274 Liquid Handler) F410513 (402 Dual Syringe Pump)
Product #s: 25101413 (Quad-Z Liquid Handler) 2506163 (Quad-Z 215 Adapter Kit for Disposable Tips) 2515154 (849 Multiple Injection Module) F410513 (402 Dual Syringe Pump)
45
Liquid Handlers
Select either a four or eight probe conguration depending on application and throughput requirements.
Ideal solution for plate-to-plate transfers.
The Multiple Probe 215 is a high-throughput automated liquid handler capable of tasks such as sample transfers, dilutions, mixing, reagent addition and microplate reformatting. The Multiple Probe 215 features patented Dual Rail Syringe Drive technology, accurate use of four or eight syringes and accommodates probes that t all application requirements.
The 223 Sample Changer is a programmable sampler for automated sample preparation and transfer. It provides a cost-effective solution for protocols such as serial dilutions, additions, sampling into vials, timed reactions and tube-to-tube transfers. The 223 Sample Changer is ideal for automated transfer of samples in analytical techniques like FIA and spectroscopy (UV/VIS, AA and ICP). Cross-contamination is minimized by adjusting rinse volumes and using liquid level sensing.
Specifications
Reproducibility 0.8% (10 L) Accuracy 98.2% (10 L)
Dimensions (w x d x h) 53.5 x 43.7 x 60 cm (21.1 x 17.2 x 24 in)
Specifications
Reproducibility 0.57% (500 L) Carryover < 1 ppb Accuracy 99.2% (500 L)
Optional ow-through rinse station, available on the left and/or right of the support bar, reduces cross-contamination.
Choose between an optional 402 Syringe Pump, Minipuls Evolution or Minipuls 3 Peristaltic Pump to add aspirating and dispensing exibility.
Dimensions (w x d x h) 97.8 x 61 x 56 cm (36 x 24 x 22 in)
Product #: 25101311 (Multiple Probe 215 Liquid Handler)
The 215 Liquid Handler is a laboratory tested workhorse for performing dilutions, aliquoting solutions, mixing, plate reformatting, reconstituting of samples and more. The 215 Liquid Handler has a punch strength capable of piercing thick or thin lm septa and microplate mats that is distinguished throughout the liquid handling industry.
Accommodates a wide variety of probes as well as standard and custom racks.

(see page 53 for the Gilson Rack/ Instrument Compatibility Chart)
Flexible open bed design allows for a variety of rack options including tubes, vials, microplates and Peltier control.
Specifications
Reproducibility 1.37% (50 L) Carryover < 1 ppb Accuracy 99.7% (50 L)
Product #s: 2510121 (215 Liquid Handler) 2510191 (215 Liquid Handler without pump)
Dimensions (w x d x h) 91 x 61 x 56 cm (36 x 24 x 22 in)
Product #: 191015 (223 Sample Changer)
47
Accessories
Switching Valve Mixing Racks Heating/Cooling Page Number 49 VALVEMATE II Valve Actuator
The VALVEMATE II Valve Actuator automates HPLC and LC techniques by increasing the system flexibility to save time, increase throughput and extend column life. It is designed to facilitate both solvent selection and column switching. The VALVEMATE II increases flexibility of HPLC systems allowing for multiple valve and multiple column configurations.
Specifications S
Dimensions (w x d x h) 8.3 x 15.6 x 9.8 cm (3.3 x 6.2 x 3.9 in) Valve Switching Speed < 180 ms port-to-port (depending on valve)
High Pressure Valves: Analytical 2-position, 6-port, stainless steel (0.016" ID ports) Analytical 2-position, 10-port, stainless steel (0.016" ID ports) Preparative 2-position, 6-port, stainless steel (0.030" ID ports) Preparative 2-position, 10-port, stainless steel (0.030" ID ports) Preparative 2-position, 6-port, PPS (0.030" ID ports) Preparative 2-position, 10-port, PPS (0.030" ID ports) Multi-position, 6-port, stainless steel (0.016" ID ports)
Low Pressure Valves: Multi-position, 6-port, PPS (0.030" ID ports) Multi-position, 6-port, PPS (0.060" ID ports) Multi-position, 8-port, PPS (0.060" ID ports) Multi-position, 10-port, PPS (0.060" ID ports)
Orbital Shaker
50
6 Column Plumbing Schematic
RackCount System
50
Multi-position, 6-port, stainless steel (0.030" ID ports) Multi-position, 6-port, PEEK (0.016" ID ports) Multi-position, 10-port, stainless steel (0.016" ID ports) Multi-position, 10-port, PEEK (0.016" ID ports)
818 AutoMix
51
852/853/854 Peltier Racks 242/542 Peltier Racks
40C 4C
51
40C 4C
52
Multi-directional keypad control with valve position LED display. Contact closure control for home and switch valve position.
Complementing robust and flexible instruments, Gilsons accessories optimize instrumentation to fit application requirements for the ultimate solution.
Fast valve switching times minimize pressure spikes and carryover.
Product #: 331052AB (VALVEMATE II Valve Actuator)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories.
49
Accessories
The Gilson Orbital Shaker is specifically designed to satisfy liquid handling needs for on-line or off-line vortexing and mixing. It is a fully functional, stand-alone instrument equipped with a single-stepper motor.
Designed for Gilsons 215 Series instruments, the 818 AutoMix provides fully automated, software-driven mixing and sampling. Typical applications that use the AutoMix include mixing whole blood samples, immiscible liquids and more.
Mix an entire rack of samples with oscillating mixing.
Specifications
Mixing Speed/Accuracy 10 rpm: 0.5 rpm 20 rpm: 1 rpm Rack Capacity (1) Code 200 Series Rack Dimensions (w x d x h) 22.9 x 50.8 x 16.5 cm (9 x 20 x 6.5 in)
Specifications
Circular Rotational Speed 20 720 rpm Rack Capacity (1) Code 200 Series Rack Dimensions (w x d x h) 11.8 x 31.6 x 4.7 cm (4.65 x 12.44 x 1.85 in)
The Orbital Shaker can be placed on the bed of the GX-281 or 215 Series instruments using the optional mounting plate kits.
Product #: 251520 (818 AutoMix)
Product #: 251711 (Orbital Shaker)
Gilsons RackCount System, numbered overlays that fit securely on the top of racks, provides a quick and effortless bed location reference for specific samples or collected fractions on a Gilson liquid handler.
RackCount overlays can be used with new rack purchases or added to existing Gilson Code 200 Series Racks. They have sequentially numbered fraction collection positions for ve complete rack positions and one position for sample locations. The numbering patterns are solvent resistant and compatible with most normal and reversed-phase solvents.
Gilson offers flexible, accurate and programmable racks for heating and cooling valuable samples; cooling to reduce evaporation and maintain sample integrity, heating to facilitate reactions and improve kinetics.
Gilson offers a variety of standard and custom rack configurations. Up to ve racks can be controlled from a single programmable Peltier controller integrated with a system or as a stand-alone unit.
Precise temperature control from 4 to 40C.
Specifications
Rack Capacity 852: (2) 96-well microplates, shallow 853: (96) 12 x 32 mm (2 mL) at-bottom vials 854: (2) 96-well microplates, deep Dimensions (w x d x h) 11.9 x 33.8 x 16.8 cm (4.7 x 13.3 x 6.6 in)
Product #s: 2514852 (far left) (852 Peltier Rack) 2514853 (center left) (853 Peltier Rack) 2514854 (center right) (854 Peltier Rack) 2515850 (far right) (Peltier Controller)
Visit www.gilson.com or call 608-836-1551 for more details on Gilson Accessories.
51
Accessories Reference
Gilson Rack/Instrument Compatibility Chart
GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 PREPFC FC 204 FC 203B Orbital Shaker Vessel Capacity (mL) Vessel Diameter
Vessel Quantity
Rack Product # Capacity Description
0 1 2 4 7 8 9 11 14
270430
80 - 12 x 32 mm (2 mL) vials
2 6 or 9 1 5 or 8 X 0.3 0.7 9 5 or 8 X X X X 4.5 9 25 4.5 to 32 6.8 4 20 3.5 5 or 7 1.5 0.5 2 2 0.7 0.7 6 or 9 20 20
80 80 119 80 1 120 120 105 128 1 4 4 8 108 60 44 44 44 44 14 108 60 60 60 60 60 108 108 60 14 14
12 13 6 12
32 65 or 100 50 75 or 100
Vessel Height
Microplate
Gilson Peltier Racks are individually controlled to cool and heat microplates on the bed of a Gilson liquid handler. The racks provide accurate temperature control from 4 to 40C.
Rack Code
The combination of a 542 and a 242 Peltier Rack can accommodate over 1,000 samples on a 215 Liquid Handler.
A single Peltier controller is able to power a 542 and a 242 Peltier Rack or up to ve 242 Peltier Racks.
X X X X
12040101 80 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 130402 130412 2707401 270438 270439 170413 170414 170415 170416 170416D 170418 150425 150422 150424 150498 150426 270433 150427 150420 150429 2704342 2704341 2704430 119 - 6 x 50 mm (1 mL) tubes 80 - 12 x 75 mm (5 mL) or 12 x 100 mm (8 mL) tubes 1 - 96-well microplate, shallow at-bottom 120 - 6 x 32 mm (0.3 mL) vials 120 - 7 x 40 mm (0.7 mL) vials 105 - 13 x 100 mm (9 mL) tubes 128 - 12 x 75 mm (5 mL) or 12 x 100 mm (8 mL) tubes 1 - 96-well microplate, shallow 4 - 96-well microplates, shallow 4 - 96-well microplates, deep 8 - 24, 48 or 96-well microplates, Whatman Deep Polyltronics Plates 108 - 10 x 100 mm (4.5 mL) tubes 60 - 13 x 100 mm (9 mL) tubes 44 - 18 x 150 mm (25 mL) tubes 44 - 10 x 100 mm (4.5 mL) to 18 x 180 mm (32 mL) tubes 44 - 17 x 55 mm (6.8 mL) scintillation vials 44 - 15 x 45 mm (4 mL) WISP vials 14 - 28 x 57 mm (20 mL) scintillation vials 108 - 10 x 75 mm (3.5 mL) tubes 60 - 12 x 75 mm (5 mL) or 13 x 75 mm (7 mL) tubes 60 - (1.5 mL) Eppendorf vials 60 - (0.5 mL) Eppendorf vials 60 - 12 x 32 mm (2 mL) vials
X 6 7 13 12 32 40 100 75 or 100 X X X X X X X X X X 10 13 18 10 to 18 17 15 28 10 12 or 13 100 100 150 100 to 180 55 45 57 75 75 X X X X X X X X X X X X X X X X X X X X 12 12 7 7 13 28 28 32 32 40 40 65 or 100 57 57 X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
Specifications
Product #s: 25146331 (left) (242 Peltier Rack) 2514542 (center) (542 Peltier Rack) 2515331 (right) (215 Peltier Controller)
Rack Capacity 242: (2) 96-well microplates, shallow 542: (10) 96-well microplates, shallow Dimensions (w x d x h) 242: 11.4 x 30.5 x 12.4 cm (4.5 x 12 x 4.9 in) 542: 50.1 x 33.6 x 11.4 cm (20.1 x 13.3 x 4.5 in)
15 16 16D 17 20 21 22 22U 23 23W 24 28 29 29LE 29SE 30 30P 31 31P 32
2704530P 60 - 12 x 32 mm (2 mL ) vials 2704431 108 - 7 x 40 mm (0.7 mL) vials
2704531P 108 - 7 x 40 mm (0.7 mL) vials 2704432 2704433 60 - 13 x 65 mm (6 mL) or 13 x 100 mm (9 mL) tubes 14 - 28 x 57 mm (20 mL) scintillation vials
Gilson offers thousands of standard and custom racks to meet the needs of any and all applications. A variety of vessels can be accommodated including tubes, vials, microplates and microchips.
33 33P
2704533P 14 - 28 x 57 mm (20 mL) scintillation vials
Visit www.gilson.com or call 608-836-1551 for more details on Gilson Instruments and Software.
53
Reference
Gilson Rack/Instrument Compatibility Chart continued
GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 PREPFC FC 204 FC 203B Orbital Shaker Vessel Capacity (mL) GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 PREPFC FC 204 FC 203B Orbital Shaker Vessel Capacity (mL)
Vessel Diameter
Vessel Quantity
Vessel Diameter
Vessel Quantity
Vessel Height
Microplate
Rack Code
34 34P 35P 36P 37 81 91 92
2704434
36 - 15 x 45 mm (4 mL) WISP vials
4 4 2 + 20 0.7 + 20 2 + 20 X 500 1000 9 12 3 6 9 X X 1.5 + 9 1.5 + 9 1.5 or 2.5 3 20 20 X X 1.5 + 9 1.5 + 9 7 12 25 2 10 125 125 125 9+7
36 36 71 + 1 120 + 1 56 + 1 2 11 8 60 39 20 8 96 2 + 2 +2 2+2+2 96 96 27 24 2+2+2 2+2+2 96 75 70 96 75 9 8 9 48 + 48
15 15 12 + 28 7 + 28 12 + 28
45 45 32 + 60 41 + 60 32 + 60
X X X X X X X X
213 214 216 217 218 219 220 222
2504613 2504614 2504616 2504617 2504618 2504619 2504620 2504622
2704534P 36 - 15 x 45 mm (4 mL) WISP vials 71 - 12 x 32 mm (2 mL) vials + 2704535P 1 - 28 x 60 mm (20 mL) vial 120 - 7 x 41 mm (0.7 mL) vials + 2704536P 1 - 28 x 60 mm (20 mL) vial 2704437 1904301 56 - 12 x 32 mm (2 mL) vials + 1 - 28 x 60 mm (20 mL) vial 2 - 96-well microplates, shallow
37 - 16 x100 mm (12 mL) tubes + 37 - 16 x 75 mm (10 mL) Vacutainer tubes 48 - 10.25 x 47 mm (2.5 mL) tubes + 48 - 10.25 x 64 mm (3 mL) Vacutainer tubes 60 - 15 x 45 mm (4 mL) WISP vials 96 - 10 x 75 mm (4 mL) tubes 2 - 96-well microplates, shallow or deep + 8 - 10 x 75 mm X (4 mL) tubes; also holds Beckman modular reservoirs 27 - 30 x 95 mm (40 mL) vials 70 - 17 x 62 mm (7 mL) vials 27 - 29 x 115 mm (50 mL) conical bottom tubes
12 + 10 2.5 + 3 4 4 4 40 7 50 50 12 27 45 4 500 or 700
37 + 37 48 + 48 60 96 2+8 27 70 27 24 96 192 30 96 4 2 2
16 + 16 10.25 + 10.25 15 10 10 30 17 29 29 16 8.5 25 15
100 + 75 47 + 64 45 75 75 95 62 115 115 100 41 150 45
Vessel Height
Microplate
Rack Code
X X X X X X X X X
X X X
X X X X X X X X X X X X X X X X X X X
X X X X X X X X X
X X X X X X X X X
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
17041950 11 - Kimax or Pyrex (500 mL) bottles 17041951 8 - Kimax or Pyrex (1000 mL) bottles
121 260440086 60 - 13 x 100 mm (9 mL) tubes 122 260440087 39 - 16 x 100 mm (12 mL) tubes 143 146 200 201 2954843 2704146 2504600 2504601 20 - (3 mL) DECs 8 - (6 mL) DECs 96 - 13 x 100 mm (9 mL) Vacutainer tubes
13 16
100 100
X X X X
X X X X X X X X X
X X X X X X X
X X
24 - 29 x 115 mm (50 mL) conical bottom tubes, 222F 2504622F w/ funnel drain 223 224 225 2504623 2504624 2504625 2504626 2504628 2504634 2504635 2504637 2504639 96 - 16 x 100 mm (12 mL) tubes, w/screw caps 192 - 8.5 x 41 mm (27 mL) vials 30 - 25 x 150 mm (45 mL) vials 96 - 15 x 45 mm (4 mL) WISP vials 4 - (500 mL or 700 mL) bottles 2 - Tipack boxes (10 L or 200 L tips) 2 - Tower Pack boxes (10 L or 200 L tips) 2 - 384-well microplates 40 - 25 x 200 mm (70 mL) tubes X X X X
X X X X X X X X X X
X X X X
X X X X X
13 13 13 10.25 10.25 28 28 13 13 13 16 18 12 16 48 48 48 13 + 13
100 100 100 47 64 57 57 100 100 75 100 150 32 75 113 113 113 100 + 75
X X X
X X X X
226 228 234 235 237 239 242 300 301 302 303 304 305 306 307
2 - 96-well microplates + 2 - (1.5 mL) microcentrifuge tubes + 2 - 13 x 100 mm (9 mL) tubes 2 - 96-well microplates, w/covers + 2 - (1.5 mL) 201H 2504601H microcentrifuge tubes + 2 - 13 x 100 mm (9 mL) tubes 96 - 10.25 x 47 mm (1.5 mL) microcentrifuge tubes or 202 2504602 (2.5 mL) Vacutainer tubes 203 204 2504603 2504604 96 - 10.25 x 64 mm (3 mL) tubes 27 - 28 x 57 mm (20 mL) scintillation vials
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
X X X X X X X X X X X X X X X X X X X X
X X X X X X
X X X
2 70 40 2 1 1 1+2 3+2 3+5 3+4 6+5 6+4 2 25 40 96 + 1 96 + 1 48 + 48 48 + 48 48 + 48 32 + 32 20 + 20 20 + 20 60 44 14 12 12 12 15 12 15 12 18 32 32 75 45 75 45 32 150 25 200
X X X X X X X X X X X X X X
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
X X X
204F 2504604F 24 - 28 x 57 mm (20 mL) scintillation vials 2 - 96-well microplates, deep + 2 - (1.5 mL) Eppendorf 205 2504605 vials + 2 - 13 x 100 mm (9 mL) tubes 2 - 96-well microplates, deep w/covers + 2 - (1.5 mL) 205H 2504605H Eppendorf vials + 2 - 13 x 100 mm (9 mL) tubes 206 207 208 209 210 211 2504606 2504607 2504608 2504609 2504610 2504611 96 - 13 x 75 mm (7 mL) Vacutainer tubes 75 - 16 x 100 mm (12 mL) Vacutainer tubes 70 - 18 x 150 mm (25 mL) tubes 96 - 12 x 32 mm (2 mL) vials 75 - 16 x 75 mm (10 mL) Vacutainer tubes 9 - 48 x 113 mm (125 mL) bottles
25146331 2 - 96-well microplates, shallow 2504300 2504301 2504302 2504303 2504304 2504305 2504306 2504307 96 - (1 mL) cartridges + 1 - 96-well microplate, deep 96 - (1 mL) cartridges + 1 - 96-well microplate, shallow 48 - (1 mL) cartridges + 48 - 12 x 32 mm (2 mL) vials 48 - (3 mL) cartridges + 48 - 12 x 32 mm (2 mL) vials 48 - (3 mL) cartridges + 48 - 12 x 75 mm (5 mL) tubes 32 - (3 mL) cartridges + 32 - 15 x 45 mm (4 mL) vials 20 - (6 mL) cartridges + 20 - 12 x 75 mm (5 mL) tubes 20 - (6 mL) cartridges + 20 - 15 x 45 mm (4 mL) vials
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
X X X X X X X X X
X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X X
211F 2504611F 8 - 48 x 113 mm (125 mL) bottles, w/funnel drain 211H 2504611H 9 - 48 x 113 mm (125 mL) bottles, w/cover 212 2504612 48 - 13 x 100 mm (9 mL) tubes + 48 - 13 x 75 mm (7 mL) Vacutainer tubes
X X X X
X X
X X
X X
330 260440031 60 - 12 x 32 mm (2 mL) vials 332 260440079 44 - 18 x 150 mm (25 mL) tubes 334 260440081 14 - (40 mL) scintillation vials
55
Reference
Gilson Rack/Instrument Compatibility Chart continued
GX-281 LH/Inj GX-271 LH/Inj 215 LH/Inj Multiple Probe 215 LH/Inj GX-274 LH GX-271 LH 223 Sample Changer Quad-Z 215 LH Quad-Z 215 (Disposable Tips) GX-274 ASPEC GX-271 ASPEC SPE 215 PREPFC FC 204 FC 203B Orbital Shaker Vessel Capacity (mL)
Probes
Gilson offers many different types of probes, probe tips and coatings to meet any and all application needs providing solutions for all liquid handling requirements.
Tip Design Description The tip is cut perpendicular to the length of the probe. Features Aspirates the last drop of liquid Does not pierce septum No injection
Vessel Diameter
Vessel Quantity
Vessel Height
335 260440083 48 - 15 x 45 mm (4 mL) WISP vials 336 260440094 2 - 96-well microplates, shallow or deep 337 260440095 4 - 96-well microplates, shallow or deep 338 260440106 64 - 12 x 32 mm (2 mL) vials 341 260440039 108 - 10 x 75 mm (3.5 mL) tubes 343 260440025 80 - 13 x 100 mm (9 mL) tubes 345 260440041 44 - 16 x 150 mm (20 mL) tubes 371 373 376 502 505 2604701 2604702 2604703 2504652 2504651 36 - (1 mL) DECs 20 - (3 mL) DECs 15 - (6 mL) DECs 100 - 12 x 32 mm (2 mL) vials (1 - HP 1100LC tray) 10 - 96-well microplates, shallow or deep X X X X X X
Microplate
Rack Code
Flat
48 2 4
15
45
X X X
X X X X X X X X X X X X X X X X
X X X X X X X X X X X X X X X X X X X
X Constricted
The tip of the probe has a smaller outer diameter than the rest of the shaft. Constricted-tip probes accommodate unique tip designs optimizing every application. Compatible with injection ports
2 3.5 9 20 1 3 6 2
64 108 80 44 36 20 15 100 10 10 17 10
12 10 13 16
32 75 100 150
X X X X X X X
Beveled
The tip is cut at an angle, leaving a bevel.
Sharp tip enables septum-piercing Compatible with injection ports
12
32
X X X X X
X X X X X X X X X X X X X X Vented
Two styles: 1. A shallow groove cut in the probe shaft, above the tip (shown). 2. Two probes are assembled concentrically, creating two separate channels, the inner channel for liquid and the outer channel for gas. Vented probes may have dierent tip designs. Sharp-tip enables septum-piercing Vent allows pressures inside and outside a sealed vessel to equilibrate as liquid is dispensed or aspirated
X X X X
Spring Loaded
Low volume, touch-o dispensing.
505H 2504651H 10 - 96-well microplates, shallow or deep w/covers 517 542 821 822 823 824 825 852 853 854 VR M 2504653 2514542 2504821 2504822 2504823 2504824 2504825 2514852 2514853 2514854 2514701 1907152 17 - 96-well microplates, shallow or deep 10 - 96-well microplates, shallow 48 - (1 mL) tabbed SPE cartridges 96 - (1 mL) tabless SPE cartridges 48 - (3 mL) tabless SPE cartridges 1 - 96-well wide SPE plate, compatible with porvair-style plates such as Varian & Waters 1 - 96-well narrow SPE plate, compatible with plates such as 3M & Ansys Spec. 2 - 96-well Becton Dickinson Falcon "U-bottom" microplates, shallow 96 - 12 x 32 mm (2 mL) vials, at-bottom 2 - 96-well Beckman or Ritter microplates, deep SPE extraction plates: Waters OASIS, 3M Empore and Varian VersaPlate 223 Maldi Rack, 1 - Bruker MALDI Plate
Ideal for MALDI spotting applications Spring loaded probe removes any potential of damaging spotting surface
1 1 3
48 96 48 1 1
X 2 X
2 96 2 12 32
X X X X X X X X X X
X X Side-entry X X X X X X Deflected
The liquid ow path opens on the side of the probe, not at the tip.
X X X X X X
Recommended for thick septa Prevents coring of septa Compatible with injection ports Not recommended with limited volume
Based on the beveled tip design, the probe tip is curved and very sharp.
Sharp-tip enables septum-piercing Tip pushes septa out of the way as it pierces to prevent coring
Gilson Probe Product Number Identification

Gilson probes are laser engraved with their product number on the collar of each probe providing a quick and effortless identification reference for re-ordering and support.
Conical
Designed for PEEK injection ports. Capable of piercing thin septa Aspirates the last drop of liquid
57
Reference
Gilson Probe Selection Guide

Product # 175 mm Z-arm 2507214 Constricted, at Stainless steel 269 x 1.8 x 1.4 GX Z-Injection Module 819 (requires Injection Port #25051015) 849 (requires Injection Port #25051213) GX Direct Injection Module (requires Injection Port #26035410) 819 (requires Injection Port #2954674) 849 #25051215 Seal #2954674 GX Direct Injection Module (requires Injection Port #26035410) GX-271, GX-274 GX-281, 215, 215 SW, Multiple Probe 215 GX-271, GX-274, GX-281, 215, 215 SW, Multiple Probe 215, Quad-Z 215 Septum-piercing Tip Shape Material Dimensions (mm, L x OD x ID) Injection Compatibility Instrument Compatibility Product # Septum-piercing Tip Shape Material Dimensions (mm, L x OD x ID) Injection Compatibility Instrument Compatibility 125 mm Z-arm continued 2507242 2507243 2507244 2507245 2507248 GX-271, GX-274, GX-281, 215, 215 SW, Multiple Probe 215, Quad-Z 215 215 SPE 215 819 (requires Injection Port #270728) GX Z-Injection Module 819 (requires Injection Port #295640) 849 #25051215 GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #250510153) 849 (requires Injection Port Bar #250512862) GX Z-Injection Module GX Z-Injection Module GX Z-Injection Module 819 (requires Injection Port #2954640) GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #25051015) GX Z-Injection Module 819 (requires Injection Port #25051015) GX Z-Injection Module 819 (requires Injection Port #25051015) GX Z-Injection Module 819 (requires Injection Port #25051015) GX Z-Injection Module GX-271, GX-274, GX-281, Quad-Z 215 GX-271, GX-274, GX-281, 215, 215 SW, Quad-Z 215 GX-271, GX-274, GX-281, GX-271 ASPEC, GX-274 ASPEC, 215, 215 SW, Quad-Z 215 GX-271, GX-274, 215, 215 SW, Multiple Probe 215, Quad-Z 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215, 215 SW 27067382 X Beveled Stainless steel 221 x 2 x 0.8 Tip: 2.1 x 1.5 x 0.8 2507252 Micro septum X X X X Deected Deected Deected Deected Conical, microvolume Constricted, beveled Stainless steel, Teon interior Stainless steel, Teon interior Stainless steel Stainless steel Stainless steel Stainless steel 274 x 1.8 x 0.8 274 x 1.8 x 0.8 274 x 1.8 x 0.8 274 x 1.8 x 0.8 220 x 2.1 x 0.4 220 x 1.5 x 0.4 Tip: 10 x 0.7 x 0.4 GX Z-Injection Module GX Z-Injection Module GX Z-Injection Module GX Z-Injection Module 841 819 (requires Injection Port #25051213) 849 #250512861 819 (requires Injection Port #2954640) GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #25051015) 849 (requires Injection Port Bar #250512862) GX Direct Injection Module (requires Injection Port #26035410) 819 (requires Injection Port #2954640) 849 #250512863 GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #2954640) 849 #250512863 GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #2954640) GX Direct Injection Module (requires Injection Port #26035411) 819 (requires Injection Port #2954640) 849 #250512863 GX Direct Injection Module (requires Injection Port #26035411) GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 215 215, Multiple Probe 215 GX-271, GX-274, GX-281, GX-271 ASPEC, GX-274 ASPEC, 215
2507215
Constricted, at
Stainless steel
269 x 1.3 x 0.8
2507216
Beveled, level-sensing
Stainless steel
269 x 1.5 x 1.1 Tip: 1.6 x 1.2 x 0.8
2507247 2507249 2507253 2507254
Beveled Conical, micro-volume Beveled, levelsensing Flat, level-sensing
Stainless steel Stainless steel Stainless steel Stainless steel
323 x 1.8 x 0.8 269 x 2.4 x 0.4 269 x 1.5 x 0.4 Tip: Con Bev Micro 0.6 269 x 1.8 x 1.4
2507256
Beveled
Stainless steel
221 x 1.5 x 0.4
Micro septum
2507414
Constricted, at
Stainless steel
220 x 1.3 x 0.8
GX-271, GX-274, GX-281, 215, Multiple Probe 215
2507255
Beveled, levelsensing
Stainless steel
269 x 1.5 x 0.4
27067361
Beveled
Stainless steel
220.5 x 1.5 x 1.1
GX-271, GX-274, GX-281, 215, Multiple Probe 215
25073645
Beveled
Stainless steel Stainless steel, Teon coated (plastic tip) Stainless steel Stainless steel
269 x 1.3 x 0.8
27067373
Constricted, at
Stainless steel
221 x 1.5 x 1.1
GX-271, GX-274, GX-281, 215, Multiple Probe 215 232 XL/233 XL
2507555 251646 Inert Gas Probe 25075551 X
Flat Beveled Flat
272.5 x 2.7 x 0.8 Tip: 10 317 x 1.8 x 0.8 256.6 x 2.7 x 0.8
GX-271, GX-274, GX-281, 215, Multiple Probe 215 (183 mm arm)
26017050
Beveled
Stainless steel
269 x 2 x 0.8 Tip: 1.5 OD x 0.8
27067383
Beveled
Stainless steel
221 x 1.5 x 0.4
GX-271, GX-274, GX-281, 215, Multiple Probe 215 (183 mm arm) , GX-271 GPC Systems
125 mm Z-arm 2507234 X Side-entry Stainless steel 274 x 1.8 x 0.8 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215 GX-271, GX-274, GX-281, 215
56 mm Z-arm 496215 27067251 27067113 27067131 27067133 123 mm Z-arm 27067211 27067212 27067213 X Beveled Side-entry Tapered, at Stainless steel Stainless steel Stainless steel 160.5 x 1.5 x 1.1 160.5 x 1.5 x 1.1 160.5 x 1.5 x 1.1 223, 221 XL, Requires Injection Port #2954645 231 XL/232 XL/233 XL, ASTED XL 223, 221 XL X X X Flat Beveled Constricted, at Beveled Beveled Stainless steel Stainless steel Stainless steel Stainless steel Titanium 51 x 0.7 x 0.4 103 x 0.7 x 0.4 93.5 x 1.5 x 1.1 95 x 0.7 x 0.42 95 x 0.7 x 0.42 Requires Injection Port #270728 & Probe Sleeve #27067132 Requires Injection Port #270728 Requires Sleeve #27067132 Requires Injection Port #2954645 231 XL GX-271, GX-274, 223, 231 XL 223, 231 XL/232 XL/ 233 XL 223, 231 XL/232 XL/ 233 XL
2507235
Side-entry
Stainless steel
274 x 1.8 x 0.8
2507236
Side-entry
Stainless steel
272.5 x 1.5 x 0.8
2507237
Side-entry
Stainless steel
273 x 1.8 x 0.8
59
Reference
Gilson Probe Selection Guide continued

Product # Septum-piercing Tip Shape Material Dimensions (mm, L x OD x ID) 157.5 x 0.9 (18 g) Tip: 10 157.5 x 0.9 (22 g) Tip: 10 162 x 0.7 x 0.42 Injection Compatibility Instrument Compatibility 123 mm Z-arm continued 27067217 27067218 27067231 Flat Flat Tapered, beveled Teon coated (plastic tip) Teon coated (plastic tip) Stainless steel 223 223 Requires Injection Port #270728 223, 231 XL/232 XL/233 XL, & Probe Sleeve #27067232 ASTED XL Requires Injection Port #270728 223, 231 XL/232 XL/233 XL, & Probe Sleeve #27067236 ASTED XL Requires Injection Port #270728 231 XL, & Probe Sleeve #27067239 ASTED XL 223, Requires Injection Port #270728 231 XL/232 XL/233 XL, ASTED XL 223, Requires Injection Port #2954645 231 XL/232 XL/233 XL, ASTED XL
1A 1 H 1.008 3 Li 6.941 11 Na 23.00 19 K 39.10 37 Rb 85.47 55 Cs 132.9 87 Fr (223)
8A
Periodic Table of the Elements

2A 3A 4A 5A 7 N 14.01 15 P 30.97 33 As 74.92 51 Sb 121.8 83 Bi 209.0 115 Uup (288) 6A 8 O 16.00 16 S 32.06 34 Se 78.96 52 Te 127.6 84 Po (209) 116 Uuh (291) 7A 9 F 19.00 17 Cl 35.45 35 Br 79.90 53 I 126.9 85 At (210) 117 Uus
Not Yet Observed
27067235 27067237 27067238
X X X
Beveled Beveled Beveled
Stainless steel Titanium Stainless steel
162 x 0.7 x 0.42 162 x 0.7 x 0.42 162 x 0.7 x 0.4
27067272 27167215 Double probe kit 27367513 183 mm Z-arm 27067361 X
Tapered, beveled Beveled Flat
Stainless steel Stainless steel Teon coated (plastic tip)
160.5 x 1.5 x 1.1 probe 1: 181.5 x 1.5 x 1.1 probe 2: 169.5 x 2.5 x 1.1 157.5 x 0.9 Tip: 10
4 5 6 Be B C 9.012 10.81 12.01 12 13 14 Mg Al Si 4B 5B 1B 2B 26.98 28.09 6B 7B 8B 24.31 3B 24 25 26 27 20 21 22 23 28 29 30 31 32 Cr Mn Fe Co Ca Sc Ti V Ni Cu Zn Ga Ge 40.08 44.96 47.90 50.94 52.00 54.94 55.85 58.93 58.70 63.55 65.38 69.72 72.59 42 43 44 45 38 39 40 41 46 47 48 49 50 Mo Tc Ru Rh Sr Y Zr Nb Pd Ag Cd In Sn 87.62 88.91 91.22 92.91 95.94 (98) 101.1 102.9 106.4 107.9 112.4 114.8 118.7 74 75 76 77 56 72 73 78 79 80 81 82 W Re Os Ir Ba Hf Ta Pt Au Hg Tl Pb * 137.3 178.5 180.9 183.9 186.2 190.2 192.2 195.1 197.0 200.6 204.4 207.2 109 88 104 106 105 107 108 110 111 112 113 114 Une Ra Rf Unh Ha Uns Hs Ds Rg Uub Uut Uuq ** 226.0 (261) (262) (263) (262) (277) (276) (281) (280) (285) (284) (289) 58 59 60 57 Ce Pr Nd La 138.9 140.1 140.9 144.2
2 He 4.003 10 Ne 20.18 18 Ar 39.95 36 Kr 83.80 54 Xe 131.3 86 Rn (222) 118 Uuo (294)
*Lanthanides
Beveled, levelsensing X Side-entry Constricted, at, level-sensing Flat Stainless steel 220.5 x 1.5 x 1.1 819 (requires Injection Port #2954640) 232 XL/233 XL (requires Injection Port #2954645) 215, 223, 232 XL/233 XL
61 62 63 64 65 66 67 68 69 70 71 Pm Sm Eu Gd Tb Dy Ho Er Tm Yb Lu (145) 150.4 152.0 157.3 158.9 162.5 164.9 167.3 168.9 173.0 175.0 95 Am (243) 96 Cm (247) 97 Bk (247) 98 Cf (251)
Synthetic
**Actinides
27067362
Stainless steel
220.5 x 1.5 x 1.1 221 x 1.5 x 1.1 Tip: 2 x 1.1 x 0.4 220.5 x 0.9
27067373 27367423 210 mm Z-arm 26037001 X
Stainless steel Teon coated
Requires Injection Port #2954645 223, 232 XL/233 X 819 (requires Injection Port #2954640) 215, Multiple Probe 215, 849 #250512863 223, 232 XL/233 XL 889 #250512753 232 XL/233 XL GX Direct Injection Module (requires Injection Port #26035411) GX Direct Injection Module (requires Injection Port #26035411) GX Direct Injection Module (requires Injection Port #26035411) GX Direct Injection Module (requires Injection Port #26035411) GX Direct Injection Module (requires Injection Port #26035411) GX Direct Injection Module (requires Injection Port #26035411)
90 91 92 93 94 89 Th Pa U Np Pu Ac 227.0 232.0 231.0 238.0 237.0 (244)
99 Es (252)
100 Fm (257)
101 Md (258)
102 No (259)
103 Lr (260)
Undiscovered
From Decay
Primordial
Beveled
Stainless steel Stainless steel (coated) Stainless steel Stainless steel (coated) Stainless steel
304 x 2 x 0.8 Tip: 2.1 x 1.5 x 0.8 301.3 x 2 x 0.8 Tip: 2.1 x 1.5 x 0.8 304 x 1.5 x 0.4
Flow Cell Selection Chart for Optimizing Sensitivity on 15X Series Detectors
GX-281
Selecting the correct flow cell will optimize sensitivity and peak shape. The following chart pairs flow cells with the appropriate column, flow rate and sample concentration to help choose the correct flow cell for the application.
Capillary Flow Cell Micro-bore Flow Cell 104442 1.5 L 2 mm 1 2 mm Analytical Flow Cell 104445 12 L 5 mm 4 mm Semi-preparative Flow Cell 104441 0.7 L 0.2 mm 20 mm Preparative Flow Cell 104443 0.16 L 0.05 mm > 40 mm
260370011
Beveled
GX-281 Product # Internal Volume GX-281 Pathlength Column Size GX-281 104446 35 nL 8 mm 180 300 m
26037002
Beveled
GX-281
260370021
Beveled
301.3 x 1.5 x 0.4
26037004
Beveled
304 x 1.5 x 0.4
26037005 26037006 26037007
Beveled Flat Flat
Stainless steel Stainless steel Stainless steel
304 x 1.5 x 1.1 304 x 2.4 x 1.8 304 x 1.8 x 1.4
GX-281 GX-281 GX-281
10 mm
Flow Rate
1 10 L/min
25 200 L/min
1 15 mL/min Purify g - 5 mg
15 50 mL/min Purify 5 300 mg
30 200 mL/min Purify 300 mg - 15 g
61
Reference
pH of Acids and Bases
pH of Common Acids
Acids (pH < 7) Acetic Acetic Acetic Alum Arsenious Benzoic Boric Carbonic Citric Formic Hydrochloric Hydrochloric Hydrochloric Hydrocyanic Hydrogen Sulde Lactic Malic Nitric Orthophosphoric Oxalic Succinic Salicylic Sulfuric Sulfuric Sulfuric Sulfurous Tartaric Trichloracetic Molarity 1N 0.1 N 0.01 N 0.1 N Saturated 0.1 N 0.1 N Saturated 0.1 N 0.1 N 1N 0.1 N 0.01 N 0.1 N 0.1 N 0.1 N 0.1 N 0.1 N 0.1 N 0.1 N 0.1 N Saturated 1N 0.1 N 0.01 N 0.1 N 0.1 N 0.1 N pH 2.4 2.9 3.4 3.2 5 3 5.3 3.8 2.1 2.3 0.1 1 2 5.1 4.1 2.4 2.2 1 1.5 1.3 2.7 2.4 0.3 1.2 2.1 1.5 2 1.2 Bases (pH > 7) Ammonia Ammonia Ammonia Barbital Sodium Borax Calcium Carbonate Calcium Hydroxide Ferrous Hydroxide Lime Magnesia Potassium Acetate Potassium Bicarbonate Potassium Carbonate Potassium Cyanide Potassium Hydroxide Potassium Hydroxide Potassium Hydroxide Sodium Acetate Sodium Benzoate Sodium Bicarbonate Sodium Carbonate Sodium Hydroxide Sodium Hydroxide Sodium Hydroxide Sodium Metasilicate Sodium Sesquicarbonate Trisodium Phosphate
Maximum UV Absorbance for Commonly Used Solvents

pH of Common Bases
Molarity 1N 0.1 N 0.01 N 0.1 N 0.01 N Saturated Saturated Saturated Saturated Saturated 0.1 N 0.1 N 0.1 0.1 N 1N 0.1 N 0.01 N 0.1 N 0.1 N 0.1 N 0.1 N 1N 0.1 N 0.01 N 0.1 N 0.1 N 0.1 N pH 11.6 11.1 10.6 9.4 9.2 9.4 12.4 9.5 12.4 10.5 9.7 8.2 11.5 11 14 13 12 8.9 8 8.4 11.6 14 13 12 12.6 10.1 12 Solvent 1-Nitropropane 2-Butoxyethanol Acetone Acetonitrile Amyl Alcohol Amyl Chloride Benzene Butanol-1 Butanol-2 n-Butyl Chloride Carbon Disulde Carbon Tetrachloride Chloroform Clorobenzene Cyclohexane Cyclopentane Diethyl Amine Dimethyl Sulfoxide Dioxane Ethanol Ethyl Acetate Ethyl Ether Ethyl Sulde Ethylene Dichloride Ethylene Glycol Heptane Hexane Isooctane Isopropanol Isopropyl Chloride Isopropyl Ether Methanol Methyl Acetate Methyl Ethyl Ketone Methyl Isobutyl Ketone Methylene Chloride Typical Flow Rates (mL/min) 0.5 2 4 15 10 50 40 100 100 300 400 1000 n-Pentane n-Propanol n-Propyl Chloride Nitromethane Petroleum Ether Pyridine Tetrahydrofuran Toluene Xylene Maximum UV Absorbance (nm) 380 220 330 190 210 225 280 215 260 220 380 265 245 287 200 200 275 268 215 210 256 220 290 230 210 200 195 215 205 225 220 205 260 330 334 233 190 210 225 380 210 330 230 285 290 Triethylamine, 1% Triuoracetic Acid, 0.1% TRIS HCl, 20 mM pH 7.0 pH 8.0 235 190 202 212 Sodium Formate 200 Sodium Citrate, 10 mM Sodium Dodecyl Sulfate 225 190 Sodium Chloride, 1M 207 MES, 10 mM, pH 6.0 Potassium Phosphate Monobasic, 10 mM Dibasic, 10 mM Sodium Acetate, 10 mM 215 190 190 205 EDTA, Disodium, 1 mM HEPES, 10 mM, pH 7.6 Hydrochloric acid, 0.1% 190 225 190 Ammonium Acetate, 10 mM Ammonium Bicarbonate, 10 mM Diammonium Phosphate, 50 mM 205 190 205
Operating at a wavelength near or below a mobile phase solvents maximum UV absorbancy increases baseline noise due to the UV response of the solvent. Refer to the tables below to optimize chromatographic conditions. Table 1 Table 2
Mobile Phase Conditioners & Buffers Acetic Acid, 1% Maximum UV Absorbance (nm) 230
Column Sizes and Capacities

Internal Diameter (mm) 4.6 10 21.2 30 50 100 Typical Injection Volume (L) 15 100 400 1,000 2,000 10,000 Load Capacity (mg) 1 4.7 19.5 42.5 118 473
63
Reference
Optimizing Preparative HPLC

There are numerous parameters and techniques to optimize preparative HPLC. These reference tables provide important physical parameters of the tubing and injection valve structure; optimization will improve chromatography and percent recovery of collected fractions. Table 1: 33X Series pump tubing (PEEK)* At high flow rates tubing can cause high back pressure. Ensuring that the appropriate tubing ID/flow rate combination is used will optimize the pumping system.
*30" lengths of tubing used at corresponding ow rates for tubing pressure. Tubing was connected directly to the pump. All pre-lters were removed. Flow Rate (mL/min) 25 50 75 100 125 150 175 200 Tubing ID (in) 0.02 Pressure (psi) 0 43.5 101.5 203.1 333.6 464.1 623.7 797.7 0.03 Pressure (psi) 0 0 0 14.5 43.5 87 101.5 145 0.04 Pressure (psi) 0 0 0 0 0 14.5 14.5 29
Solvent Miscibility and Polarity

Solvent Miscibility Table
Solvent Polarity Refraction UV(nm) Boiling Viscosity Solubility Index Index Cut-off Point (cPoise) in water @20C @1AU (C) (%w/w) 6.2 5.1 5.8 2.7 4.0 3.9 1.6 4.1 0.2 3.5 3.1 6.4 7.2 4.8 5.2 4.4 2.8 0.0 0.0 5.1 2.5 4.7 0.0 4.0 3.9 2.2 4.0 2.4 1.0 9.0 2.5 1.372 1.359 1.344 1.501 1.394 1.399 1.466 1.446 1.426 1.444 1.424 1.431 1.478 1.422 1.360 1.372 1.353 1.387 1.375 1.329 1.369 1.379 1.358 1.384 1.377 1.368 1.407 1.496 1.477 1.333 1.500 230 330 190 280 254 215 263 245 200 225 235 268 268 215 210 260 220 200 200 205 210 329 200 210 210 220 215 285 273 200 290 118 56 82 80 125 118 77 61 81 84 41 155 189 101 78 77 35 98 69 65 55 80 36 97 82 68 65 111 87 100 139 1.26 .032 0.37 0.65 0.73 2.98 0.97 0.57 1.00 0.79 0.44 0.92 2.00 1.54 1.20 0.45 0.32 0.39 0.33 0.60 0.27 0.45 .23 2.27 2.30 0.37 0.55 0.59 0.57 1.00 0.61 100 100 100 0.18 0.43 7.81 0.08 0.815 0.01 0.81 1.6 100 100 100 100 8.7 6.89 0.0003 0.001 100 4.8 24 .004 100 100 100 0.051 0.11 100 0.018
Table 2: Rheodyne 7010 156 Factory Installed Large-bore Rotor Seal

Valve Position and Pressure Flow Rate (mL/min) Standard 7010 Bore Size Load Position Pressure (psi) 0 0 0 14.5 43.5 87 Inject Position Pressure (psi) 14.5 43.5 87 145 290 435.1 7010 with Large-bore Rotor Gilson Installed Load Position Pressure (psi) 0 0 0 14.5 72.5 145 Gilson Installed Inject Position Pressure (psi) 0 14.5 43.5 116 203.1 435.1 696.2
Xylene Water Tichloroethylene Toluene Tetrahydrofuran Di-Iso-Propyl Ether Iso-Propanol6 n-Propanol Pentane Methyl Ethyl Ketone5 Methyl-t-Butyl Ether4 Methanol Hexane Heptane Di-Ethyl Ether Ethyl Acetate Ethanol Dioxane Dimethyl Sulfoxide3 Dimethylformamide Dichloromethane2 1,2-Dichloroethane1 Cyclohexane Chloroform Carbon Tetrachloride Butyl Acetate n-Butanol Benzene Acetonitrile Acetone Acetic Acid
25
Product #: 25051043 (7010 valve with large-bore rotor seal) 0.018" make-before-break stator orice 1 mL stainless steel 0.030" ID loop All other plumbing used is stainless steel 0.040" ID tubing
50 75 100 150 200
Acetic Acid Acetone Acetonitrile Benzene n-Butanol Butyl Acetate Carbon Tetrachloride Chloroform Cyclohexane 1,2-Dichloroethane1 Dichloromethane2 Dimethylformamide Dimethyl Sulfoxide3 Dioxane Ethanol Ethyl Acetate Di-Ethyl Ether Heptane Hexane Methanol Methyl-t-Butyl Ether4 Methyl Ethyl Ketone5 Pentane n-Propanol Iso-Propanol6 Di-Iso-Propyl Ether Tetrahydrofuran Toluene Tichloroethylene Water Xylene Immiscible Miscible
Immiscible means that in some proportions two phases will be produced.
Synonym Table Ethylene Chloride Methylene Chloride 3 Methyl Sulfoxide 4 tert-Butyl Methyl Ether 5 2-Butanone 6 2-Propanol
1 2
Separation Scale-up Equations

Scale-up Factor (diameter preparative)2 __________________________ scale-up factor = (diameter analytical)2 Flow Rate scale-up factor flow rate preparative = __________________________ analytical flow rate Gradient Duration (GD) length preparative (diameter preparative)2 flow rate analytical __________ _____________ ____________ GD preparative = GD analytical x x x 2 length analytical (diameter analytical) flow rate preparative
Polar Relative Polarity Non-polar Compound Formula R-H Ar - H R-O-R Increasing Polarity R-X R - COOR R - CO - R R - NH 2 R - OH R - COHN 2 R - COOH H - OH
Solvent Polarity Chart

Group Alkanes Aromatics Ethers Alkyl Halides Esters Aldehydes and Ketones Amines Alcohols Amides Carboxylic Acids Water Representative Solvent Compounds Petroleum Ethers, Ligroin, Hexanes Toluene, Benzene Diethyl Ether Tetrachloromethane, Chloroform Ethyl Acetate Acetone, Methyl Ethyl, Ketone Pyridine, Triethylamine Methanol, Ethanol, Isopropanol, Butanol Dimethylformamide Ethanoic Acid Water
65
Reference
Common Conversion Tables
Temperature Conversion
Celsius (C) -100 -50 -40 -30 -20 -10 0 5 10 15 20 25 30 35 40 45 50 55 60 65 70 75 80 85 90 95 100 105 110 115 120 125 130 135 140 145 150 155 160 165 170 175 180 185 190 195 200 Fahrenheit (F) -148 -58 -40 -22 -4 14 32 41 50 59 68 77 86 95 104 113 122 131 140 149 158 167 176 185 194 203 212 221 230 239 248 257 266 275 284 293 302 311 320 329 338 347 356 365 374 383 392 25/32 13/16 27/32 7/8 29/32 15/16 31/32 1 .7813 .8125 .8438 .875 .9063 .9375 .9688 1 19.8438 20.6375 21.4313 22.225 23.0188 23.8125 24.6063 25.4 0.042 0.055 0.062 1.067 1.397 1.575 1067 1397 1575 22.703 38.933 49.474 0.8938 1.5328 1.9478 9/32 5/16 11/32 3/8 13/32 7/16 15/32 1/2 17/32 9/16 19/32 5/8 21/32 11/16 23/32 3/4 .2813 .3125 .3438 .375 .4063 .4375 .4688 .5 .5313 .5625 .5938 .625 .6563 .6875 .7188 .75 7.1438 7.9375 8.7313 9.525 10.3188 11.1125 11.9063 12.7 13.4938 14.2875 15.0813 15.875 16.6688 17.4625 18.2563 19.05 0.009 0.01 0.011 0.012 0.013 0.014 0.015 0.016 0.017 0.018 0.019 0.02 0.028 0.03 0.032 0.04 0.229 0.254 0.279 0.305 0.33 0.356 0.381 0.406 0.432 0.457 0.483 0.508 0.711 0.762 0.813 1.016 229 254 279 305 330 356 381 406 432 457 483 508 711 762 813 1016 1.042 1.287 1.557 1.853 2.175 2.523 2.896 3.295 3.72 4.17 4.646 5.148 10.09 11.583 13.179 20.593 0.041 0.0507 0.0613 0.073 0.0856 0.0993 0.114 0.1297 0.1464 0.1642 0.1829 0.2027 0.3973 0.456 0.5189 0.8107 Prefix yotta zetta exa peta tera giga mega
Fractional inch-to-decimal-to-millimeter Conversion

Fraction (") 1/32 1/16 3/32 1/8 5/32 3/16 7/32 1/4 Decimal .0313 .0625 .0938 .125 .1563 .1875 .2188 .25 Millimeter .7938 1.5875 2.3813 3.175 3.9688 4.7625 5.5563 6.35 Inch 0.001 0.002 0.003 0.004 0.005 0.006 0.007 0.008
Tubing Internal Diameters & Volumes

Millimeter 0.025 0.051 0.076 0.102 0.127 0.152 0.178 0.203 Micron 25 51 76 102 127 152 178 203 L/in 0.013 0.051 0.116 0.206 0.322 0.463 0.631 0.824 L/mm 0.0005 0.002 0.0046 0.0081 0.0127 0.0182 0.0248 0.0324 kPa 690 3450 6890 10340 13790 17240 20680 24130 27580 31030 34470 37920 41370 44820 48260 51710 55160 58610 62050 65500 68950
Pressure Conversion
psi 100 500 1000 1500 2000 2500 3000 3500 4000 4500 5000 5500 6000 6500 7000 7500 8000 8500 9000 9500 10000 bar 6.9 34.5 68.9 103.4 137.9 172.4 206.8 241.3 275.8 310.3 344.7 379.2 413.7 448.2 482.6 517.1 551.6 586.1 620.5 655 689.5 MPa 0.7 3.4 6.9 10.3 13.8 17.2 20.7 24.1 27.6 31 34.5 37.9 41.4 44.8 48.3 51.7 55.2 58.6 62.1 65.5 68.9
Metric Prefixes
Symbol Y Z E P T G M k h da d c m n p f a z y Scientific Notation 1024 1021 1018 1015 1012 109 106 103 102 10 10-1 10-2 10-3 10-6 10-9 10-12 10-15 10-18 10-21 10-24
Temperature Conversion Formulas

C = F 32 1.8 C = K 273.15 F = 1.8 x C + 32 F = R 459.67 K = C + 273.15 R = F + 459.67 C = degrees Celsius F = degrees Fahrenheit K = degrees Kelvin R = degrees Rankine
kilo hecto deka deci centi milli micro nano pico femto atto zepto yocto
67
Reference
Gilson Applications
Liquid Chromatography
cLC System: An Automated Solution for Normal and Reversed-phase Preparative HPLC with Analytical Purification Determination The cLC system offers researchers the capabilities of both RP and NP chromatography on a single system, including data representing the throughput and analysis capabilities of the system. Optimizing Purified Sample Recovery on Reversed-phase HPLC Systems This application presents the factors that can directly affect purity and recovery when processing compounds on a preparative level. The following system parameters were evaluated: collection valve switch time, detector time constant delay, DMSO sandwich solvent, delay volume from detector to collection valve and software response. Optimizing Chiral Separations via an Automated Parallel Scouting System for On-line Preparative Chiral Purification The system is capable of screening chiral compounds on four separate columns in parallel, increasing the stereoselective screening by four fold. Determination of Reproducibility within an Analytical System via Repeat Injections The data presented in this application shows that the Gilson HPLC system provides consistent and reproducible chromatography. A mixture of three compounds was injected repeatedly under analytical reversed-phase conditions. A Multi-purpose Preparative System for the Detection and Collection of Active and Non-active UV/VIS Compounds The process relies on an analytical scout analysis to determine the best gradient conditions to optimize the separation of the compounds under preparative conditions and minimize the number of fractions collected per run by incorporating ELS detection in addition to UV/VIS detection. Systematic Look at the Affects of Various Solvents, Injection Techniques and Sample Amounts in Preparative HPLC Columns Data is presented documenting the affects elicited in preparative chromatography under various conditions. A Totally Integrated 2D System Incorporating Ion-exchange and RP-HPLC for Natural and Biological Products An automated two-dimensional application that incorporates ionexchange and reversed-phase chromatography for the separation of complex mixtures. Data is presented that shows the usefulness of two dimensional chromatography and implementing alternative chromatography modes. Novel Approach for Screening of Drug Absorption via an Automated System The system presented provides a faster alternative to other screening methods, as well as an inexpensive analysis estimate of drug permeability for hundreds of drug candidates in a fraction of the time of conventional screening methods. Preparative HPLC: Factors and Parameters that Directly Affect Recovery of Collected Fractions Parameters discussed are column performance, mobile phase constituents, detector settings and collection parameters such as collection via level versus slope and delay volumes. The data offers guidelines for all chromatographers purifying compounds by preparative HPLC and can be implemented into existing systems. Optimized Sample Load Capacity and Separation Through a Series of Short Preparative Columns By placing the smaller preparative columns in series, loading capacity and separations of compounds can increase with short run times and semi-preparative flow rates, minimizing the overall cost of the larger preparative columns and channeling within the column. Train Keep a Rolling and Isolation Gradients: A Method for Purifying a Large Quantity of Sample via Continuous Injection and Simultaneous Fraction Collection on Multiple FC Beds Train injection employs continuous injection of sample under isocratic conditions and continuous fraction collection. In addition, isolation gradients also offer an efficient purification of preparative samples when the train injection technique is not applicable, as is the case with complicated chromatograms. Separation of Flavonoids Through Automated Multiple Column Injections and Sample Concentration via C18 Column Capture Identical columns are configured in parallel for multiple injections sequenced by the sample peak elution. Sample is concentrated on a separate C18 preparative column, the purified compound is eluted in high organic solvent for ease of dry-down. Enhanced Recovery of Compound Mixtures by Concurrent Dissection and Concentration of Isolates A crude separation of a complex mixture into impure isolates. The impure isolates are reintroduced to the column. Front and back sections are cleaved to fractionate uncontaminated compound on separate columns for concentration. Final elution dispenses pure compound in high organic solvent for ease of dry-down.
Liquid Chromatography continued

Automated Post-collection Concentration for Purified Preparative Fractions via SPE The method (as opposed to analytical applications involving SPE) works by collecting and then diluting the fraction(s), passing the fraction(s) through an SPE and ultimately eluting the concentrated fraction in a small amount of 100% organic solvent. An Automated Neoteric Approach to Trapping Metabolites for NMR Analysis SPE cartridges are used to trap the metabolites from natural occurring material during a repetitive analytical run with injection of the same sample; in order to concentrate the individual metabolites to a level required for NMR analysis. A Chiral Piece of the Pie-Optimizing Chiral Separations through Selective Sample Slicing and Column Switching Samples are injected onto a primary column and individual peaks are cleaved and transferred onto a secondary chiral column for separation. We demonstrate complete purification of analytes with high resolution of the separated enantiomers Increasing Throughput of GPC Clean-Up via a Parallel Sample Extraction Process The purpose of this study was to evaluate the effectiveness of developing an automated GPC clean-up system that is capable of running up to four sample extracts in parallel, along with fraction collection, thus improving sample throughput. Optimization of Steady State Recycle SSR: Parameters Utilizing Polarimetry in Chiral Separations Processing gram to kilogram quantities of target analytes has led to the exploration of several high throughput separation techniques. Among those investigated is Steady State Recycle SSR. Similar to Simulated Moving Bed SMB fractions are collected from the leading and trailing edges of a chromatographic contour while sample material is injected into the interior. The difference SMB is a continuous process while SSR is a repetitive process. Flash: Eliminating the Concentration/Dry-down Required for a Purified Compound by Preparative Chromatography Employing an activated media packed in an SPE/Flash cartridge (60 mL/10 gm), the automated system can intelligently identify the purified fractions and compound mass 0.1 2 g, without time-consuming dry-down. Fast PCLC: A widely Applicable Technique for Laboratory Scale Preparative Separations It is often expected that prep HPLC can be satisfactorily performed via low efficiency, large diameter columns, operated under overload conditions. Evaluation of this preparative HPLC, low-tech route is not the best option. Short, fine particle columns permit baseline resolution of closely eluting compounds. Incorporating gradient elution and multicycle automation, the 1-inch bore can isolate 1 mg of each of several substances, but can also be employed to obtain up to 200 grams of a single compound. This technique, called FAST PCLC (preparative column liquid chromatography), reduces analysis time, so throughput is greater. For example, a 3 um column can handle a throughput of 1 gram in about one-sixth of the time required on a 30 um column. Multiple Column Injection and Single Concentration Column Fraction collection to increase sample volume and Speed up Dry Down Presented is an application to separate flavonoids via a multiple column injection. The sample injection is optimized for peak resolution on a Prep C18 Column. Identical columns are configured in parallel for injections sequenced by the sample peak elution of the test column. Sample is concentrated on separate C18 prep columns and eluted in high organic for effective dry down of the target sample. This technique provides high throughput of sample for each cycle decreasing separation time to purify gram quantities of target compounds. FTLC: Automated Fraction trapping of Purified Compounds from Preparative Chromatography via a Specialized Polymer Resin packed within a HPLC column This trapping method allows for fractions collected to undergo transformation into 100% organic solvent without operator intervention, drastically reducing the dry down time and eliminating hydrolysis of the compound. The HPLC trapping column has been shown to be very robust and rugged stable for 1000s of fraction trapping samples.
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Reference
Gilson Applications continued

Solid Phase Extraction
An Automated SPE Procedure and Analysis for the Extraction of Chlorpromazine and Thioridazine from Plasma This application describes an automated SPE method coupled with HPLC analysis to determine the levels of Chlorpromazine and Thioridazine in human plasma. An Automated SPE Procedure and Analysis for the Determination of Ibuprofen and Ketoprofen in Plasma This application describes an automated SPE method coupled with HPLC analysis to determine the various levels of Ibuprofen and Ketoprofen in human plasma. The Ephedra Story: An Automated SPE Procedure and Analysis for the Determination of Ephedra and Ephedra-like Compounds in Dietary Supplements This application presents the advantages of using an automated SPE method coupled with HPLC, versus manual SPE, to determine the various levels of Ephedra and Ephedra-like compounds in dietary supplements. Optimization of SPE by an Automated Method Development Procedure The system allows for the optimization of SPE process via multiple collection sites and various conditions without manual intervention. Incorporation of a trace enrichment cartridge to concentrate the eluent versus a dry-down step offers advantages to the dry-down bottle neck and analyte degradation. A Rapid Extraction and Clean-up Procedure for the Determination of Amnesic Shellfish Poison Toxins in Shellfish A rapid sample preparation procedure has been developed via SPE to determine the levels of domoic acid in shellfish. Data supporting this approach is presented along with the automated SPE procedure incorporating trace enrichment cartridges to increase sensitivity of the assay. Isolation and Determination of Capsaicin and Dihydrocapsaicin from Complex Matrices via Automated SPE and UV/ELS/MS Detection Matrices tested include dried habanero, topical analgesic cream and pepper spray. The system allows for the extraction procedure to be automatically optimized for each matrix without operator intervention, providing high recovery. Totally Automated Method for the Determination of Sudan Dyes in Food via On-line Filtration, SPE and HPLC Analysis The system presented offers a totally automated approach to the extraction of Sudan dyes from food products. The product is solubilized, filtered and SPE is performed prior to HPLC analysis, at detection limits below the EU of 0.5 1 mg/kg. Optimizing Parameters that Influence Recovery in SPE A totally automated approach to optimize every aspect of an SPE method without manual intervention on an SPE instrument via fractionation, improving recovery and reducing coefficient of variance. The Automated extraction of Acaricides from Honey via SPE With the recent decline in hive bee populations this year the investigation of acaricides in the environment have become more evident. One source of these analyses is the quantity of acaricides in honey. Automation of this separation technique would provide laboratories with an efficient method to extract the acaricides for analytical analysis. Bifenthrin and Fipronilare the acaricides extracted. SPE is utilized for this extraction with a separation via HPLC. Automated SPE Method for the Determination of Cotinine in Biological Fluid This procedure employs SPE, a new polymeric based cation exchange resin that resists drying, shows excellent flow characteristics, greater load capacity and resistance to aggressive washings. Melamine Determination in Baby Formula via SPE/HPLC The cleanup procedure for Melamine involves mix mode cation exchange solid phase extraction. Melamine is analyzed using a zwitterionic HILIC LC column. The method limit of quantitation (LOQ) for Melamine was: 100 ppb for liquid formula and 50 ppb for powdered infant formula powder, and CV of 3-10%. Automated Enrichment and Separation of Bifenthrin Enantiomers via SPE and HPLC The (-)enantiomer of bifenthrin degrades in field sediments, causing an incremental increase of the (+)enantiomer. These differences in the enantiomers of pesticides have contributed to the investigation of chiral pesticides by the EPA. In this application, we separated enantiomers of bifenthrin by use of both chiral HPLC methodology and SPE enrichment. The Examination and Automation of GPC, SPE and QuEChERS for Pesticides in Olive Oil Several techniques for the separation of pesticides from edible oils are used today to investigate the amount of pesticides in these oils. GPC, SPE and QuEChERS can be used to isolate the pesticides that are found in edible oils. Determination of Veterinary Drug residues in Fish by an Automated SPE-HPLC System This application presents a simple and automated procedure employing SPE to extract and concentrate the residues from fish tissue prior to direct HPLC analysis.
Solid Phase Extraction continued

Comparison of SPE, ILE, SLE Capabilities to Efficiently Extract Organic Compounds via an Automated System There are many sample preparation techniques available, e.g. protein precipitation, liquid-liquid extraction, dilution, solid phase extraction and immobilized liquid extraction. Every technique has its positive and yes, negative aspects, but SPE, ILE and SLE offer the greatest overall benefit based on their mechanism of isolation, media available, various matrices and automation. The Automated SPE Assay of Fipronil and its Metabolites from Bee Pollen Fipronil is used as a pesticide on crops that are pollinated by bees. Fipronilsmetabolites are toxic and study of the metabolites have been increasing. Automation of this separation technique would provide laboratories with an efficient method to extract the fipronil and its metabolites for analytical analysis. SPE is utilized for this extraction with verification via HPLC. Isolation and Determination of Flavonoids from Complex via Automated Solid Phase Extraction and UV/ELSD/MS Detection Automation allows efficient SPE method optimization. Fractionation targets specific volumes and UV/ELSD/MS allows selectivity and identification of standards and interfering peaks. Flavonoid recovery at 991% on all analytes, while SPE of all matrices eliminates interfering peaks. Optimization of On-line SPE of Biological samples via an Automated System with Trace enrichment (TEC) prior to Analysis via HPLC Incorporating the use of a trace enrichment cartridge (TEC) in conjunction with dilution prior to HPLC analysis eliminates the time consuming step associated with dry down and minimizes the dilution volume that usually causes a reduction in sample analysis. Hence the TEC increases the signal to noise ratio (increased sensitivity), which can be extremely important when analyzing trace analogues or metabolites. The Automated Extraction of Silymarin Flavanoligans in Milk Thistle Seed Anti-inflammatory and anticarcinogenic properties as well as scavenging abilities make silymarin a likely candidate for further scientific studies. This necessitates SPE extraction and analytical processing of the extract to provide information on its components. Automation of the extraction provides an efficient process to compliment investigation of silymarin flavanolignans. Ascorbic Acid and Sodium Benzoate in Orange Juice The FDA recently tested 84 soft drink products and found that 54 of them had some detectable benzene. And some had levels as high as 79.2 ppb. Federal rules specify less than 5 ppb in drinking water. This application automates the extraction of Sodium benzoate and vitamin C from orange juice and fruit drinks for analytical processing.
Automated Liquid Handling

Fully Automated System Employing Disposable Cartridges in Series to Facilitate the Clean-up of Biological Samples The system presented in this application is capable of processing large volume samples (25-200 mL range) fast and efficiently. A number of SepPaks can be linked together, (1 SepPak for every 75 mL of sample volume). The automated system consists of a series of controllable valves, to access numerous SepPaks and choices in solvents for the process, a liquid handler is employed to add the samples to the individual SPE and the product is collected via fraction collection events. A Novel Approach for the Extraction and Concentration of Compounds in Biological Fluids via On-Line Dialysis and Enrichment On-line dialysis and trace enrichment prior to analysis offers a unique approach to sample preparation The automated system introduces a biological sample to a dialysis membrane and through positive fluidics separates the drugs/compounds from the matrix and concentrates it onto a trace enrichment cartridge prior to analysis via HPLC. Quad-Z 215 with Disposable Tips: Implementing the Use of ZipTips for the Purification of Biological Samples This application demonstrates how the Quad-Z 215 automates sample clean-up using ZipTips. The Quad-Z 215 is also capable of completing numerous liquid handling techniques with or without tips under varying microplate formats. A Compact Automated Liquid Handler for Microbatch Protein Crystallography A system that incorporates fixed and disposable tips on the Quad-Z 215. Independent probe motion and the use of virtual pumps make the system very versatile in the field of protein crystallography via microbatch techniques. Accessing the E-Gel 96 System via the Quad-Z 215 with Disposable Tips This application examines the suitability of the Quad-Z 215 with disposable tips for the delivery of proteins to the wells of the E-Gel 96 gels for electrophoresis.
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Gilson, Inc. Reference
Gilson Applications continued

Designed for purification and engineered for science, Gilson, Inc. provides high quality, dependable solutions Automated Liquid Handling continued for todays demanding liquid chromatography, solid phase extraction, liquid handling and gel permeation chromatography clean-up requirements. From the legendaryReformatting Gilson Pipetman products to a range of system Procedures Employing afull Quad-Z 215 with Technical Data for the Quad-Z 215 (fixed probes) Dispensing Small Disposable Tips innovative products will revolutionize Volumes of DMSO Gilson is the element of purification for your laboratory. solutions, Gilsons The volumetric accuracy and presented show This study the accuracy and CVs for the Quad-Z 215 and ease-of-use thedetermined lab; providing the durability, flexibility, modularity the industry has precision come to data expect from Gilson.
Quality Policy It is Gilson, Inc.s policy to promote a total commitment to quality by each individual throughout the Gilson organization worldwide. It is Gilsons goal that this commitment to quality will result in complete customer satisfaction with both Gilsons products and services. ISO 9001 Certification Gilson is committed to quality, and it shows in everything Gilson does from design to manufacturing, technical support and training. Due to this commitment to quality and customer satisfaction, Gilson has consistently been awarded with ISO 9001 certification. Gilson, Inc. and Gilson S.A.S. are both ISO 9001 certified. Training Gilson provides an extensive program of instrument and software training for users. Standard courses as well as sessions customized to the users specific needs are offered at Gilsons innovative training center or in the users own laboratory. Computer assisted training courses are also available on CD-ROM or the Gilson on-line education center, www.training.gilson.com.
that the Quad-Z 215 with disposable tips offers researchers the (fixed probes) for low volume dispensing of DMSO. The results consistency they require in all assays. The use of common tubes of the testing show the Quad-Z 215 is capable of transferring Gilson worldwide sales format and service supporting biotech, environmental, fooda and and microplates, along with the optionclinical, of designing custom low volumes ofhas DMSO from a 96-well into an empty, flat-the pharmaceutical, forensic markets. Our primary focus is on purifying samples via preparative HPLC, automated SPE or through rack, provides a high degree of versatility to accommodate bottomed 96-well microplate. variations in with procedures. GPC clean-up. Gilson focuses on early drug discovery and development our preparative HPLC systems. Quad-Z 215 with Tips: Volume transfer Data Gilson automated SPE systems and GPC clean-up systems support environmental, food, clinical and forensic Data presented for low volume transfers with tips
markets performing a wide variety of clean-up applications.

Gel Permeation Chromatography Clean-up Gilson offers complete solutions including the revolutionary control(GPC) software, TRILUTION. TRILUTION LC Software is a complete HPLC software package designed specifically to meet purification needs. TRILUTION LH Software is to analysis for semivolatile organic compounds. Semivolatiles Determination of Chlorinated Pesticides in Poultry Fat Using GPC and designed to makewith automation of liquid handling and SPE applications simple and dependable. were extracted from soils using pressurized fluid extraction Optional Alumina Clean-up GC-ECD Detection
(USEPA Method 3545). The extracts were then purified using GPC Poultry Fat is routinely monitored for the presence of chlorinated Gilson, Inc. world headquarters is located incommon Middleton, WI and is responsible for research, development and Clean-up (USEPA method 3640A). Analysis was performed by GC/ pesticides. Gel permeation chromatography (GPC) is a MS. Recoveries for a variety of semivolatiles were tool formanufacturing the post-extraction removal of high molecular weight of all Gilson instrumentation and software in addition to providing direct sales to the U.S. determined. interferents prior to pesticide analysis. GPC removes these Gilson located outside of Paris, is responsible for all development and manufacturing of Gilson interferents viaS.A.S., a size separation mechanism thatFrance, uses organic The Examination and Automation of GPC, SPE and QuEChERS for pipetting productsgel and laboratory products. Gilson solutions are sold solvents and a hydrophobic (abenchtop cross-linked divinylbenzenePesticides in Olive Oil and supported worldwide. styrene copolymer) to separate the interferents from the lower Several techniques for the separation of pesticides from edible oils molecular weight compounds of interest. The interferents are are used today to investigate the amount of pesticides in these discarded to waste and the fraction containing the pesticides is oils. GPC, SPE and QuEChERS can be used to isolate the pesticides collected for further clean-up and analysis. GPC clean-up may be that are found in edible oils. Each technique provides strengths followed by additional clean-up procedures such as adsorption for its separation of esticides. GPC has the ability to process large chromatography using alumina, Florisil or silica. amounts of sample, SPE provides disposable cartridges with numerous sorbents to provide separation of the analyte from the matrix, and QuEChERS involves uncomplicated sample cleanup of Increasing Throughput of Gel Permeation Chromatography (GPC) pesticides in aqueous matrices. This application investigates each Clean-up via a Parallel Sample Extraction Process of these separation techniques in separating pesticides from oil Post-extraction GPC clean-up is used extensively for preparing matrices and presents detailed information on the automation of food and environmental samples (such as soils) prior to analysis each separation system. for insecticides, fungicides, herbicides, semi-volatiles, PCBs and other environmental contaminants. GPC clean-up has many benefits, including decreased instrument maintenance costs, Increasing Throughput of GPC Clean-up via a parallel Sample higher quality of analytical data, enhanced analytical column life extraction process, collection Window Determination and Individual and its capabilities of being automated. One of the disadvantages collection per Column of GPC clean-up is the relatively slow throughput per sample The purpose of this study was to evaluate the effectiveness extract compared to other sample clean-up methods. The purpose of developing an automated GPC clean-up system that is of this study was to evaluate the effectiveness of developing an capable of running 2-4 individual sample extracts in parallel, automated GPC clean-up system that is capable of running up to along with fraction collection per column, thus improving four sample extracts in parallel, along with fraction collection, thus sample throughput. Data is presented using a USEPA GPC improving sample throughput. calibration standard to determine the time window for fraction collection. A very simple acquisition software program is used to determine this collection window. One established unique food/ Automated Gel Permeation Chromatography (GPC) Clean-up of Soil environmental samples can be injected simultaneously onto the Extracts Prior to Analysis for Semivolatile Organic Compound by GC/ individual stainless steel GPC columns with independent fraction MC (USEPA Method 8270) collection per column. The Gilson Automated GX-271 GPC Clean-up System was used to perform the post-extraction clean-up of soil extracts prior
Gilson, Inc. World Headquarters 3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com Gilson S.A.S. 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE ANCE Tel: 33-(0)1-34295000 | Fax: 33-(0)1-34295020
Gilson, Inc. World Headquarters | 3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA | Tel: 608-836-1551 or 800-445-7661 | Fax: 608-831-4451 Gilson S.A.S. | 19, avenue des Entrepreneurs | BP 145, F-95400 VILLIERS LE BEL, FRANCE www.gilson.com | sales@gilson.com | service@gilson.com | training@gilson.com 2009 Gilson, Inc. | LT303041-10 | Specications subject to change without notice.

Gilson Product Guide

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Gilson, Inc.

Solid Phase Extraction Systems 35 39

3000 Parmenter Street | P.O. Box 620027 | Middleton, WI 53562-0027, USA

References sales@gilson.com | service@gilson.com | training@gilson.com | hr@gilson.com | oem@gilson.com

HPLC: Fraction Collectors

PREPFC Fraction Collector.....................................................26 FC 203B/FC 204 Fraction Collectors ..................................27

TRILUTION LC Software..............................................................29 TRILUTION LH Software .............................................................30

GPC Clean-up Systems

Gilson HPLC Systems

GX-271 Fraction Trapping HPLC System

Maximum Injection Pressure 5,000 psi (345 bar)

Binary gradient preparative HPLC pumps provide high-ow capabilities.

Mid-to-large collection capacity in a small footprint.

Modular detector options available.

Mid-to-large bed capacity, accommodating both samples and fractions.

Binary gradients are achieved with analytical to semi-preparative HPLC pumps.

Maximum Injection Pressure 5,000 psi (345 bar)

Detector Variable Dual Wavelength

Optional breakthrough detector and secondary fraction collector

Product #: 21120001(GX-271 Fraction Trapping System)

28 mm 70 Note: tubes up to 200 mm tall can be accommodated

Trapping Columns 10 mm ID x 150 mm 21.2 mm x 150 mm

HPLC: Liquid Handlers/Injectors

GX-281 Liquid Handler/Injector Bed Capacity

Accuracy 98% (100 L 25 mL)

Injection Carryover < 0.005%

98% (100 L 25 mL)

Sample Quantity 576 576

Injection, collection and analysis

GX-271 Analytical Liquid Handler/Injector

98% (10 L 25 mL)

Note: tubes up to 220 mm tall can be accommodated

GX-271 Preparative Liquid Handler/Injector

98% (50 L 25 mL)

215 Liquid Handler/Injector

Multiple Probe 215 Liquid Handler/Injector

Fraction Zone, 11 Fraction Zone, 12

Fraction Zone, 7 Fraction Zone, 8

HPLC: Liquid Handlers/Injectors

300 250 200

750 500 250 0 -250 0 2 4 6

Injection 1 2 3 4 5 6 7 8 9 10 Average Recovery Standard Deviation CV

Peak Theophylline Caffeine Phenol

Retention Time (minutes) 2.8 4.2 8.4

Area Count (Vmin/100) 14901136.11 12267689.70 13314605.43

Standard Deviation 44928.68 36503.01 73087.21

CV 0.30% 0.30% 0.55%

28 mm 70 Note: tubes up to 200 mm tall can be accommodated

HPLC: Liquid Handlers/Injectors

Pressure 3,040 psi (210 bar)

Customize by adding accessories and components to meet laboratory needs.

Column ID Page Number

H1: 0.15 15 mL/min H2: 0.3 30 mL/min

30X Series HPLC Pumps

10 L/min 200 mL/min

402 Syringe Pumps

1 L/min 120 mL/min

Minipuls Evolution Peristaltic Pump

0.2 L/min 340 mL/min

Minipuls 3 Peristaltic Pump

standard: 72 psi (5 bar) high-ow rate: 42 psi (3 bar)

Multiple Injection Module Option

Product #: 2515154 (849 Multiple Injection Module)

Retention Time Repeatability