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elcome to the January March edition of our quarterly Newsletter.

Newsletter

Unified Wine and Grape Symposium 2013 Clodagh Stafford, our Technical Sales Representative will be attending this years Unified Wine and Grape Symposium in Sacramento on behalf of Megazyme. You will find her at the trade show in booth number 1737, so if you have any questions or queries please pop over to say hello and she will be happy to help!

The New and Improved Megazyme Website 2013 is shaping up to be an exciting year at Megazyme with many new plans and projects already underway. Just this week we have launched the brand new Megazyme website with many new customer focused features making our products and resources more readily accessible. Why not log on and have a look for yourself on: www.megazyme.com Key Improvments
New Design Streamlined Ordering Online Resources Media Centre Easy Navigation

Wishing you all a very Happy and Prosperous New Year! In this issue we bring you news of Megazymes recent award, keep you up-to-date with our recent and upcoming events and take a look at some of our products in more detail. Innovation Exporter of the Year Megazyme International has won the Irish Exporters Associations Innovation Exporter of the Year Award 2012. This award recognises companies that have made a significant innovative contribution to exporting resulting in a new technology, product or service, or a new use for an existing product. The ceremony was held in University College Dublin and Mr Terence ORourke of KPMG presented Megazymes CEO, Dr. Barry McCleary with the prestigious award.

Clodagh Stafford, Megazyme

Megazyme Turns Pink for Breast Cancer On the 19th of October, the staff at Megazyme had a Pink day in order to raise funds for research into Breast Cancer. We came to work dressed in our finest pink attire and had a bake sale full of pink cakes and treats. We raised a total of 220 and a fun day was had by all.

Dr. McCleary & Colin Lawlor - IEA President

Megazymes Pink Day supporting Breast Cancer Research

Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland Tel: + 353 1 2861220 Fax: + 353 1 2861264 Email: info@megazyme.com Web: www.megazyme.com

Setting New Standards

Enzymes & Kits for Fructan Analysis & Research

We are pleased to announce that we can now offer ultra-pure, recombinant, affinity purified endo-inulinase (E-ENDOIAN) and exo-inulinase (E-EXOIAN). These enzymes have been produced at our research facility in Bray, Ireland. They are available separately for research applications and have also been incorporated into the Fructan and Fructan HK analytical kits offered by Megazyme. Megazyme now offers highly pure, recombinant exo-Inulinase from A. niger. Recombinant Cat. code: E-EXOIAN (EC 3.2.1.80) CAZy: GH Family 32 SPECIFIC ACTIVITY: ~ 2000 U/mg (60C, pH 4.5 on kestose); ~ 1000 U/mg (40C, pH 4.5 on kestose). RELATIVERATES OF HYDROLYSISOF SUBSTRATES: Substrate Relative Hydrolysis Rate Kestose 100 Sucrose 143 Raffinose 36 Inulin (dahlia) 11 PHYSICOCHEMICALPROPERTIES: pH Optima: 3.5 - 4.5 Temperature Optima: 50C - 60C pH Stability: 3.0 - 9.0 Temperature Stability: up to 50C Cat. Code: E-EXOIAN Content: 5000 Units at 40C Price: 127.00 / $173.00

Megazyme now offers highly pure, recombinant endo-Inulinase from A. niger. Recombinant Cat. code: E-ENDOIAN (EC 3.2.1.7) CAZy: GH Family 32 SPECIFIC ACTIVITY: ~ 580 U/mg (60C, pH 4.5 on inulin); ~ 300 U/mg (40C, pH 4.5 on inulin). RELATIVERATES OF HYDROLYSISOF SUBSTRATES: Substrate Inulin (Raftiline) (20 mg/mL) Inulin (dahlia) (10 mg/mL) Relative Hydrolysis Rate 100 ~ 95 Cat. Code: E-ENDOIAN Content: 500 Units at 40C Price: 118.00 / $160.00

PHYSICOCHEMICALPROPERTIES: pH Optima: 4.5 - 5.5 Temperature Optima: 60C pH Stability: 3.0 - 8.0 Temperature Stability: up to 40C

Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland Tel: + 353 1 2861220 Fax: + 353 1 2861264 Email: info@megazyme.com Web: www.megazyme.com

Setting New Standards

Fructan Kit (K-FRUC) suitable for the measurement of even trace levels of fructan (inulin or fructo-oligosaccharides, FOS) in plant and food products e.g. in grain and in infant formulations. The removal of interfering sugars through borohydride reduction also reduces the terminal reducing fructose in partially hydrolysed fructans. This leads to an underestimation of up to 20% for highly depolymerised fructans. However, if the pure FOS ingredient is available, the level of underestimation can be determined and allowed for. Fructan HK (K-FRUCHK) gives no underestimation of fructan, even with highly depolymerised samples. However, the method is not recommended for samples containing high levels of D-glucose, D-fructose, sucrose and/or maltose. Analytical Kits S S Fructan (K-FRUC) - AOAC Method 999.03; AACC Method 32-32.01 - Incorporating highly purified enzymes including recombinant exo-inulinase and endo-inulinase Fructan HK (K-FRUCHK) - Incorporating highly purified enzymes including recombinant exo-inulinase and endo-inulinase - For quantitative measurement of fructan and FOS

Pure Research Enzymes S S exo-Inulinase (E-EXOIAN) (recombinant, affinity purified) NEW endo-Inulinase (E-ENDOIAN) (recombinant, affinity purified) NEW

Purified Enzymes for Specific Analytical Applications S S S exo-Inulinase plus endo-Inulinase mixture (E-FRMXLQ) - For measurement of fructan according to AOAC Method 997.08 exo-Inulinase plus endo-Inulinase mixture (E-FRMXPD) - For measurement of fructan according to AOAC Method 997.08 Amyloglucosidase (E-AMGDFR100 & E-AMGFR500) - For use in fructan determination according to AOAC Method 997.08

Soluble Chromogenic Substrates S Azo-Fructan (S-AZFR5) - For the measurement of endo-inulinase

Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland Tel: + 353 1 2861220 Fax: + 353 1 2861264 Email: info@megazyme.com Web: www.megazyme.com

Setting New Standards

Pure Enzymes for Starch Research

Isoamylase (E-ISAMY)


S for complete debranching of starch and glycogen. S Ultrapure. Contaminating enzymes < 1 part in 106 (by activity).

Pullulanase (E-PULKP)

S for partial but controlled debranching of starch. S Affinity purified. Contaminating enzymes < 1 part in 105 (by activity).

b-Amylase (barley) (E-BARBL)

S removal of maltosyl units from the non-reducing ends of maltodextrin chains. S Recrystallised. Contaminating enzymes < 1 part in 106 (by activity).

a-Amylase (B. amyloliquefaciens) (E-BAASS)

S for analysing the cluster structure of starches ( Bertoft et al, 2012*). S highly purified. Contaminating enzymes < 1 part in 106 (by activity).

* Bertoft, E., Koch, K. & Aman, P. (2012) Building block organisation of clusters in amylopectins of different structural types, Int. J. Biol. Macromol., 50, 1212-1223.

Amyloglucosidase (Rhizopus sp.)

(E-AMGPU) S for complete hydrolysis of soluble starch. No action on blocked substrates. S Ultrapure. Contaminating enzymes < 1 part in 107 (by activity).

Amyloglucosidase (H. resinae) (E-GAMP)

S for complete hydrolysis of soluble starch. No action on blocked substrates. S Recombinant / affinity purified. Contaminating enzymes < 1 part in 107 (by activity).

a-Glucosidase (yeast)

(E-MALTS) S rapidly hydrolyses maltose and maltotriose. Not active on a-1,6-linked D-glucose. S Ultrapure. Contaminating enzymes < 1 part in 107 (by activity).

a-Glucosidase (B. Stearothermophilus) (E-TSAGS)

S rapidly hydrolyses all a-1,4-linked maltodextrins. S Recombinant / affinity purified. Contaminating enzymes < 1 part in 107 (by activity).

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Megazyme International Ireland, Bray Business Park, Bray, Co. Wicklow, Ireland Tel: + 353 1 2861220 Fax: + 353 1 2861264 Email: info@megazyme.com Web: www.megazyme.com