RONALD D.

VALE
University of California
San Francisco, CA 94143-2200

BIRTH: January 11, 1959; Hollywood, California

EDUCATION:
1985 Ph.D., Neurosciences, Stanford University
1980 College of Creative Studies, University of California, Santa Barbara
Majors: Biology and Chemistry

RESEARCH AND PROFESSIONAL EXPERIENCE:

2004-present Chair, Department of Cellular and Molecular Pharmacology

2000-2003 Director, Cell Biology Program, UCSF

1999-present Investigator, Howard Hughes Medical Institute

1998-present William K. Hamilton Distinguished Professorship of Anesthesia

1995-1999 Associate Investigator, Howard Hughes Medical Institute

1995-2003 Vice-Chair, Department of Cellular and Molecular Pharmacology

1994-present Professor, University of California, San Francisco

1992-1994 Associate Professor, University of California, San Francisco

1986-1992 Assistant Professor, University of California, San Francisco

1985-1986 Staff Fellow, Laboratory of Neurobiology, NINCDS, National Institutes

of Health, Department of Health and Human Services at the Marine
Biological Laboratory, Woods Hole, Massachusetts.

HONORS, AWARDS, AND SPECIAL ACTIVITIES:

2007 Daniel Mazia Memorial Lecture, Stanford University
2006 “Method to Extend Research in Time” (MERIT) award, National Institute
of General Medical Sciences, NIH
2006 Lamport Lectureship, University of Washington
2005 Finalist, Science & Engineering Visualization Challenge, National Science
Foundation and the journal Science
2005 21st Annual Kenneth F. Naidorf Memorial Lecture, Columbia Univ.
2004 Keith Porter Friday Night Lecturer, Marine Biological Laboratory
2003 46th Annual Faculty Research Lecture Award, UCSF
2002 American Academy of Arts and Sciences
2001 - Election to the National Academy of Sciences
1998- Baker Memorial Lecture, University of Michigan
1998- William K. Hamilton Distinguished Professorship of Anesthesia
1997 - Keynote speaker: 12th European Cytoskeleton Forum
1996- Davies Lectureship, University of Pennsylvania
1993- Young Investigator Award, Biophysical Society
1993- American Heart Association Established Investigator
1991- Pfizer Award in Enzyme Chemistry, American Chemical Society
1991- Nikon Fellowship, Marine Biological Laboratory
1989 - R.R. Bensley Award, American Association of Anatomists
1989- Rita Allen Fellowship
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1988 - Klingenstein Fellowship

1987 - Searle Scholar Award
1986 - Dan Fellowship, Research Exchange Program in Japan

SCIENTIFIC ACTIVITIES:
Advisory Committee, Indian Institute for Scientific Education and Reesearch, Pune, India (2008)
Scientific Advisory Board, Center for Ecosystems Survival (2008)
HHMI Investigator Referee (2008)
NIH Reviewer (2008)
Advisory Committee for the Program in Cellular Dynamics Imaging, Marine Biological
Laboratory (2007-present)
ASCB International Affairs Committee (2006-present)
NIH CDF Working Group (2003)
ASCB E.B. Wilson Award Committee Member (2003)
NIH Program Project Grant Reviewer (2003)
Co-Editor (with Thomas Kreis) of the second editions of the Guidebook to Cytoskeletal and
Motor Proteins and the Guidebook to ECM, Anchor and Adhesion Proteins (1999)
Co-Organizer of the 2000 Keystone Symposium: Dynamics of the Cytoskeleton (Feb. 3-9, 2000)
Co-Organizer of HHMI Workshop "New Technology in Cell Biology and Genomics" (1998)
NIH Study Section: Chair of CDF4 (2000-2002)
NIH Study Section: Cell Biology I (1996-1999)
ASCB Meeting: Local Program Committee (1996)
ASCB Meeting Program Committee (1998)
Editorial Board: Journal of Cell Biology (2003-present)
Editorial Board, Reviews: Journal of Cell Biology (1999-2002)
Editorial Board: Traffic (1998-present)
Editorial Board: Genes to Cell (1995-present)
Editorial Board: Current Opinion in Cell Biology (1995-present)
Editorial Board: Cell Structure and Function (1995-present)
Co-Editor (with Thomas Kreis) of the first editions of the Guidebook to Cytoskeletal and Motor
Proteins and the Guidebook to ECM and Adhesion Proteins (1993)
Ad Hoc Board Member of the Annual Review of Cell Biology (1990)
Co-Organizer of the Genentech-Glaxo-UCLA Symposium on "Cytoskeleton and Cell Regulation"
(Jan. 20-26, 1990)

EDUCATIONAL ACTIVITIES
Founder and Producer, iBioSeminars (http://www.ascb.org/ibioseminars/ )
Founder and Producer, Microscopy for Kids (http://microscopy4kids.org )
Co-Director Physiology Course, Marine Biological Laboratory (2004-2008)
Instructor, Physiology Course- Marine Biological Laboratory (1992,1993)

MAJOR UNIVERSITY EXPERIENCE

Chair, Dept. of Cellular and Molecular Pharmacology
Genentech Hall Governance Committee
Research Advisor for the Chairman and Faculty of the Dept. of Anesthesia
Medical Scientist Training Program- Council Member
Biophysics Program- Council Member
Organizer of Cell Biology/Biochemistry Retreats
Teaching
Pharmacology Lectures for Medical, Dental, and Pharmacy Students
Biochemistry Lectures for Medical Students
Cell Biology Course for Graduate Students- Course Director and Lecturer
Biochemistry Course for Graduate Students- Course Director and Lecturer
Microscopy Course for Graduate Students/Postdocs- Course Director and Lecturer
Cellular Biophysics Course- Lecturer
Neuroscience Course- Lecturer
Lecturer for High School Teachers and High School Students
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INDUSTRY EXPERIENCE
Co-Founder and Scientific Advisory Board Member, Cytokinetics, Inc.
Co-Founder and Scientific Advistory Board Member, 100x Imaging
PATENTS:
6,831,160 "Methods of affinity purifying proteins using modified bis-arsenical fluorescein"
6,699,969 "Assays for the detection of microtubule depolyermization inhibitors"
6,429,304 "Nucleic acids encoding a katanin p60 subunit"
6,410,687 "Polypeptides for the detection of microtubule depolylmerization inhibitors"
7,252,950 “Assays for detecting modulators of cytoskeletal function”

PUBLICATIONS :
1. Fox, C.F., Vale, R., Peterson, S.W. and Das, M. (1979). The EGF receptor: identification and
functional modulation. In: Cold Spring Harbor Conferences on Cell Proliferation. Vol. 6,
Book A, pp. 143-158.
2. Fox, C.F., Wrann, M., Linsley, P. and Vale, R. (1979) Hormone-induced modification of EGF
receptor proteolysis in the induction of EGF action. J. Supramol. Struct. 12: 517-531.
3. Peterson, S.W., Lerch, V., Moynihan, M.E., Carson, M.P. and Vale, R. (1982) Partial
characterization of a growth inhibitory protein in 3T3 cell plasma membranes. J. Exp. Cell
Res. 142: 447-451.
4. Vale, R.D., DeLean, A., Lefkowitz, R.J. and Stadel, J.M. (1982) Regulation of insulin
receptors in frog erythrocytes by insulin and concanavalin A: evidence for discrete classes
of insulin binding sites. Mol. Pharm. 22: 6 19-629.
5. Vale, R.D. and Shooter, E.M. (1982) Alteration of binding properties and cytoskeletal
attachment of nerve growth factor receptors in PC12 cells by wheat germ agglutinin. J. Cell
Biol. 94: 710-717.
6. Vale, R.D., Chandler, C.E., Sutter, A. and Shooter, E.M. (1983) Binding and internalization
of nerve growth factor. In: Receptors and Recognition (P. Cuatrecasas and T. Roth, eds.).
Series B, Vol. 15, pp. 83-118.
7. Vale, R.D. and Shooter, E.M. (1983) Conversion of nerve growth factor receptor complexes
to a slowly dissociating, Triton X-100 insoluble state by anti-nerve growth factor antibodies.
Biochem. 22: 5022-5028.
8. Vale, R.D. and Shooter, E.M. (1983) Epidermal growth factor receptors on PC12 cells:
alteration of binding properties by lectins. J. Cell. Biochem. 22: 99-109.
9. Sutter, A., Hosang, M., Vale, R.D. and Shooter, E.M. (1984) Interaction of nerve growth
factor and its specific receptor. In: Cellular and Molecular Biology of Neuronal
Development (I.B. Black, ed.). Plenum Press, New York, pp. 201-214.
10. Vale, R.D., Peterson, S.W., Matiuck, N.V. and Fox, C.F. (1984) Purified plasma membranes
inhibit polypeptide-induced DNA synthesis in subconfluent 3T3 cells. J. Cell Biol. 98: 1129-
1132.
11. Vale, R.D., Szent-Györygi, A. and Sheetz, M.P. (1984) Movement of scallop myosin on
Nitella actin filaments: regulation by calcium. Proc. Natl. Acad. Sci. USA 81: 6775-6778.
12. Vale, R.D., Hosang, M. and Shooter, E.M. (1985). Sialic acid residues on NGF receptors on
PC12 cells. Dev. Neurosci. 7: 55-64.
13. Vale, R.D., Ignatius, M.J. and Shooter, E.M. (1985) Association of nerve growth factor
receptors with the Triton X-100 cytoskeleton of PC12 cells. J. Neurosci. 5: 2762-2770.
14. Vale, R.D. and Shooter, E.M. (1985) Assaying binding of nerve growth factor to cell surface
receptors. Meth. Enzym. 109: 21-39.
15. Vale, R.D., Schnapp, B.J., Sheetz, M.P. and Reese, T.S. (1985) Movement of organelles along
filaments dissociated from the axoplasm of the squid giant axon. Cell 40: 449-454.
16. Schnapp, B.J., Vale, R.D., Sheetz, M.P. and Reese, T.S. (1985) Single microtubules from
squid axoplasm support bidirectional movement of organelles. Cell 40: 455-462.
17. Vale, R.D., Schnapp, B.J., Reese, T.S. and Sheetz, M.P. (1985) Organelle, bead and
microtubule translocations promoted by soluble factors form the squid giant axon. Cell 40:
559-569.
18. Vale, R.D., Reese, T.S. and Sheetz, M.P. (1985) Identification of a novel force generating
protein, kinesin, involved in microtubule-based motility. Cell 42: 39-50.
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19. Vale, R.D., Schnapp, B.J., Mitchison, T., Steuer, E., Reese, T.S. and Sheetz, M.P. (1985)
Different axoplasmic proteins generate movement in opposite directions along
microtubules in vitro. Cell 43: 623-632.
20. Vale, R.D., Schnapp, B.J., Mitchison, T., Reese, T.S. and Sheetz, M.P. (1985) Kinesin: a
novel microtubule-based force generating protein. In: Microtubules and Microtubule
Inhibitors (M. DeBrabander and J. DeMey, eds.). Vol. 3, 213-224.
21. Schnapp, B.J., Vale, R.D., Reese, T.S. and Sheetz, M.P. (1986). Organelle movement along
microtubules. New York Acad. Sci. 466: 909-918.
22. Sheetz, M.P., Vale, R., Schnapp, B., Schroer, T. and Reese, T. (1986) Vesicle movements and
microtubule-based motors. J. Cell Sci. 5: 181-188.
23. Vale, R.D., Scholey, J.M. and Sheetz, M.P. (1986) Kinesin: possible biological roles for a new
microtubule motor. Trends in Biochem. Sci. 11: 464-468.
24. Porter, M.E., Scholey, J.M., Stemple, D.L., Vigers, G.P.A., Vale, R.D., Sheetz, M.P. and
McIntosh, J.R. (1987) Characterization of microtubule movement produced by sea urchin
egg kinesin. J. Biol. Chem. 262: 2794-2802.
25. Sheetz, M.P., Vale, R.D., Schnapp, B.J., Schroer, T. and Reese, T.S. (1987) Movements of
vesicles on microtubules. New York Acad. Sci. 493: 409-416.
26. Vale, R.D. (1987) Intracellular transport using microtubule-based motors. Ann. Rev. Cell
Biol. 3: 347-378.
27. Vale, R.D. (1988) Microtubule-based mobility proteins and sorting of vesicles in neurons.
In: Molecular Mechanisms in Secretion (N.A. Thorn, M. Treiman, O.H. Petersen, eds.)
Alfred Benzon Symposium 25, Munksgaard, Copenhagen, pp. 397-406.
28. Vale, R.D. and Hotani, H. (1988) Formation of membrane networks in vitro by kinesin-
driven microtubule movement. J. Cell Biol. 107: 2233-2241.
29. Vale, R.D. and Toyoshima, Y.Y. (1988) Rotation and translocation of microtubules in vitro
induced by dyneins from Tetrahymena cilia. Cell 52: 459-469.
30. Vale, R.D. and Toyoshima, Y.Y. (1988) In vitro motility assays for kinesin and dynein. In:
Cell Movement (F.D. Warner and J.R. McIntosh, eds.) Alan Liss, Inc., Vol. 2, pp. 287-294.
31. Vale, R.D. and Toyoshima, Y.Y. (1988) In vitro translocation properties of ciliary dyneins.
In: Dynamics of Microtubules; Proceeding of the 14th Taniguchi International.
Symposium, Division of Biophysics (H. Hotani, ed.) The Taniguchi Foundation. pp. 2-16.
32. Howard, J., Hudspeth, A.J. and Vale, R.D. (1989) Movement of microtubules by single
kinesin molecules. Nature 342: 154-158.
33. McCaffrey, G. M. and Vale, R.D. (1989) Identification of a kinesin-like microtubule-based
motor protein in Dictyostelium discoideum. EMBO J. 8: 3229-3234.
34. Vale, R.D., Soll, D.R., and Gibbons, I.R. (1989) One-dimensional diffusion of microtubules
bound to flagellar dynein. Cell 59: 915-925.
35. Vale, R.D. and Toyoshima, Y.Y. (1989) Microtubule translocation properties of intact and
proteolytically digested dyneins from Tetrahymena cilia. J. Cell Biol. 108: 2327-2334.
36. Malik, F. and Vale, R. (1990) A new direction for kinesin. Nature 347: 713-714.
37. Vale, R.D. (1990). Microtubule-based motors. Curr. Opin. Cell Biol. 2: 15-22.
38. Vale, R.D. and Goldstein, L.S.B. (1990) One motor, many tails: an expanding repertoire of
force-generating enzymes. Cell 60: 883-885.
39. Vale, R.D. and Oosawa, F. (1990) Protein motors and Maxwell's demons: does
mechanochemical transduction involve a thermal ratchet? Adv. Biophys. 26: 97-134.
40. Allan, V. and Vale, R.D. (1991) Cell cycle control of microtubule-based membrane transport
and tubule formation in vitro. J. Cell Biol. 113: 347-359
41. Allan, V.J., Vale, R.D. and Navone, F. (1991) Microtubule-based organelle transport in
neurons. In: The Neuronal Cytoskeleton, (R.D. Burgoyne, ed.) Alan R. Liss, Inc., pp. 257-
282.
42. Goldstein, L.S.B. and Vale, R.D. (1991) A brave new world for dynein. Nature 352: 569-570.
43. Schliwa, M., Shimizu, T., Vale, R.D. and Euteneuer, U. (1991) Nucleotide specificities of
anterograde and retrograde organelle transport in Reticulomyxa are indistinguishable. J.
Cell Biol. 112: 1199-1203.
44. Shimizu, T., Furusawa, K., Ohashi, S., Toyoshima, Y.Y., Okuno, M., Malik, F. and Vale, R.D.
(1991) Nucleotide specificity of the enzymatic and motile activities of dynein, kinesin and
heavy meromyosin. J. Cell Biol. 112: 11898-1197.
45. Vale, R.D. (1991) Severing of stable microtubules by a mitotically-activated protein in
Xenopus egg extracts. Cell 64: 827-839.
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46. Ferreira, A., Niclas, J., Vale, R.D., Banker, G. and Kosik, K.S. (1992) Suppression of kinesin
expression in cultured hippocampal neurons using antisense oligonucleotides. J. Cell Biol.
117: 595-606.
47. Goldstein, L.S.B. and Vale, R. (1992) New cytoskeletal liasons. Nature 359: 193-194.
48. Navone, F., Niclas, J., Hom-Booher, N., Sparks, L., Bernstein, H., McCaffrey, G.M., and
Vale, R.D. (1992) Cloning and expression of a human kinesin heavy chain gene: Interaction
of the COOH-terminal domain with cytoplasmic microtubules in transfected CV-1 cells. J.
Cell Biol. 117: 1263-1275.
49. Vale, R.D. (1992). Microtubule motors: many new models off the assembly line. Trends
Biol. Sci. 17: 300-304.
50. Vale, R.D., Banker, G. and Hall, Z.W. (1992) The neuronal cytoskeleton. In: An Introduction
to Molecular Neurobiology. (Z.W. Hall, ed.). Sinauer Associates, Inc., Sunderland, MA, pp.
247-280.
51. Vale, R.D., Malik, F. and Brown, D. (1992) Directional instability of microtubule transport
in the presence of kinesin and dynein, two opposite polarity microtubule motor proteins. J.
Cell Biol. 119: 1589-1596.
52. Vale, R.D., Radeke, M.J., Misko, T.P. and Shooter, E.M. (1992) The nerve growth factor
receptors. In: Receptor Subunits and Complexes. (e.d A. Burgen and E.A. Barnard).
Cambridge University Press, Cambridge, U.K., pp. 391-417.
53. McNally, F. and Vale, R.D. (1993) Identification of katanin, an ATPase that severs and
disassembles stable microtubules. Cell 75: 419-429.
54. Romberg, L. and Vale, R.D. (1993) Chemomechanical cycle of kinesin differs from that of
myosin. Nature 361: 168-170.
55. Scholey, J.M. and Vale, R.D. (1993) Kinesin based organelle transport. In: Microtubules (J.S.
Hyans and C.W. Lloyd, eds.) John Wiley & Sons, pp. 343-365.
56. Shimizu, T., Toyoshima, Y.Y., Vale, R.D. (1993) Use of ATP analogs in motor assays. Meth.
Cell Biol. 39: 167-177.
57. Vale, R.D. (1993) Measuring single motor proteins at work. Science 260: 169-170.
58. Vale, R.D. (1993). Motor proteins. In: Guidebook to Cytoskeletal and Structural Proteins.
(R. Vale and T. Kreis, eds.) Oxford University Press, pp. 175-183.
59. Wilhelm, J. and Vale, R.D. (1993) RNA on the move: The mRNA localization of pathway. J.
Cell Biol. 123: 269-274.
60. Allan, V. and Vale, R.D. (1994) Movement of membrane tubules along microtubules in
vitro: evidence for specialized sites of motor attachment. J. Cell Sci. 107: 1885-1897.
61. Malik, F., Brillinger, D. and Vale, R.D. (1994) High resolution tracking of microtubule
motility produced by single kinesin motors. Proc. Natl. Acad. Sci. U.S.A. 91: 4584-4588.
62. Niclas, J., Navone, F., Hom-Booher, N. and Vale, R.D. (1994) Cloning and localiization of a
conventional kinesin motor expressed exclusively in neurons. Neuron 12: 1059-1072.
63. Vale, R.D. (1994) Getting a grip on myosin. Cell 78: 733-737.
64. Vale, R.D., Coppin, C.M., Malik, F., Kull, F.J. and Milligan, R.A. (1994) Tubulin GTP
hydrolysis influences the structure, mechanical properties and kinesin-driven transport of
microtubules. J. Biol. Chem. 269: 23769-23775.
65. Coppin, C.M., Finer, J.T., Spudich, J.A. and Vale, R..D. (1995) Measurement of the isometric
force exerted by a single kinesin molecule. Biophys. J. 68: 242s-244s.
66. Hoenger, A., E.P. Sablin, R.D. Vale, R.J. Fletterick, and Milligan, R.A. (1995) Three-
dimensional structure of a tubulin-motor protein complex. Nature 376: 271-274.
67. Shimizu, T., Sablin, E., Vale, R.D., Fletterick, R., Petchatnikova, E. and Taylor, E.W. (1995)
Expression, purification, ATPase and microtubule binding properties of the ncd domain
ADP release is the rate limiting step in the microtubule-stimulated ATPase pathway.
Biochem. 34: 13259-13266.
68. Shimizu, T., Toyoshima, Y.Y., Edamatsu, M. and Vale, R.D. (1995) Comparison of the
motile and enzymatic properties of two microtubule minus-end-directed motors, ncd and
cytoplasmic dynein. Biochem. 34: 1575-1582.
69. Coppin, C.M., Finer, J.T., Spudich, J.A., and Vale, R.D. (1996) Detection of sub-8 nm
movements of kinesin by high resolution optical trap microscopy. Proc. Natl. Acad. Sci.
U.S.A. 93: 1913-1917.
70. Fowler, V.M. and Vale, R.D. (1996) Cytoskeleton (Editorial Review). Curr. Opin. Cell Biol.
8: 1-3.
71. Kull, F.J., Sablin, P., Lau, R., Fletterick, R.J. and Vale, R.D. (1996) Crystal structure of the
kinesin motor domain reveals a structural similarity to myosin. Nature 380: 550-555.
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72. McNally, F.J. and Vale, R.D. (1996). Katanin, the microtubule severing ATPase, is
concentrated at centrosomes. J. Cell Sci. 109: 561-567.
73. Niclas, J., Allan, V.J. and Vale, R.D. (1996) Cell cycle regulation of dynein association with
membranes modulates microtubule-based organelle transport. J. Cell Biol. 133: 585-593.
74. Sablin, E.P., Kull, F.J., Cooke, R., Vale, R.D. and Fletterick, R.J. (1996) Crystal structure of
the motor domain of the kinesin-related ncd. Nature 380: 555-559.
75. Vale, R.D. (1996) Switches, latches and amplifiers: common themes of G proteins and
molecular motors. J. Cell Biol. 135: 291-302.
76. Vale, R.D., Funatsu, T., Pierce, D.W., Romberg, L., Harada, Y. and Yanagida, T. (1996)
Direct observation of single kinesin molecules moving along microtubules by fluorescence
microscopy. Nature 380: 451-453.
77. Vignali, G., Niclas, J., Sprocati, M.T., Vale, R.D., Sirtori, C. and Navone F. (1996) Differential
expression of ubiquitous and neuronal kinesin heavy chains during differentiation of
neuroblastoma and PC12 cells. Eur. J. Neurosci. 8: 536-544.
78. Wilhelm, J. and Vale, R.D. (1996). A one-hybrid system for detecting RNA-protein
interactions. Genes to Cells 1: 317-323.
79. Case, R.B., Pierce, D.W., Hom-Booher, N., Hart, C.L. Vale, R.D. (1997) The directional
preference of kinesin motors is specified by an element outside of the motor catalytic
domain. Cell 90: 959-966.
80. Coppin, C.M., Pierce, D.W., Hsu, L. and Vale, R.D. (1997) The load dependence of kinesin's
mechanical cycle. Proc. Natl. Acad. Sci. 94: 8359-8544.
81. Funatsu, T., Harada, Y., Higuchi, H., Tokunaga, M., Saito, K., Ishii, Y., Vale, R.D. and
Yanagida, T.(1997). Imaging and nano-manipulation of single bio-molecules. Biophys.
Chem. 68: 63-72
82. Pierce, D.W., Hom-Booher, N. and Vale, R.D. (1997) Imaging individual green fluorescent
proteins. Nature 388: 338.
83. Sosa, H., Dias, D.P.,Hoenger, A., Whittaker, M., Wilson-Kubalek, E., Sablin, E., Fletterick,
R.J., Vale, R.D. and Milligan, R.A. (1997) A model for the microtubule-Ncd motor protein
complex obtained by cryo-electron microscopy and image analysis. Cell 90: 217-224.
84. Takizawa, P.A., Sil, A., Swedlow, J.R., Herskowitz, I. and Vale, R.D. (1997) Actin-dependent
localization of an RNA encoding a cell-fate determinant in yeast. Nature 389: 90-93.
85. Tripet, B., Vale, R.D. and Hodges, R.S. (1997) Demonstration of coiled-coil interactions with
the kinesin neck region using synthetic peptides. J. Biol. Chem. 272: 8946-8956.
86. Vale, R.D. and Fletterick, R.J. (1997) The design plan of kinesin motors. Ann. Rev. Cell
Dev. Biol. 13: 745-777.
87. Woehlke, G., Ruby, A.K., Hart, C.L., Ly, B., Hom-Booher, N. and Vale, R.D. (1997)
Microtubule interaction site of the kinesin motor. Cell 90: 207-216.
88. de Hostos, E.L., McCaffrey, G., Sucgang, R., Pierce, D.W. and Vale, R. D. (1998). A
developmentally regulated kinesin-related motor protein from Dictyostelium discoideum.
Mol. Biol. Cell 9: 2093-2106.
89. Hartman, J.J., Mahr, J., McNally, K., Okawa, K., Iwamatsu, A., Thomas, S., Cheesman, S.,
Heuser, J., Vale, R.D. and McNally, F.J. (1998). Katanin, a microtubule-severing protein, is a
novel AAA ATPase that targets to the centrosome using a WD40-containing subunit. Cell
93: 277-287.
90. Kull, F.J., Vale, R.D. and Fletterick, R.J (1998). The case for a common ancestor: kinesin and
myosin motor proteins and G proteins. J. Muscle Res. Cell Motil. 19: 877-886.
91. Lohman, T.M., Thorn, K. and Vale, R.D. (1998) Staying on track: common features of DNA
helicases and microtubule motors. Cell 93: 9-12.
92. Pierce, D.W. and Vale, R.D. (1998) Detection of single molecule GFP fluorescence. Meth.
Enzymol. 298: 154-171.
93. Pollock, N., Koonce, M.P., de Hostos, E.L. and Vale, R.D. (1998) In vitro microtubule-based
organelle transport in wild-type Dictyostelium and and cells overexpressing a truncated
dynein heavy chain. Cell Motil. Cytoskel. 40: 304-314.
94. Prahlad, V., Yoon, M., Moir, R.D., Vale, R.D., and Goldman, R.D. (1998). Rapid movements
of vimentin on microtubule tracks: kinesin-dependent assembly of intermediate filament
networks. J. Cell Biol. 143: 159-170.
95. Romberg, L, Pierce, D.W. and Vale, R.D. (1998) Role of the kinesin neck region in
processive microtubule-based motility. J. Cell Biol. 140: 1407-1416.
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96. Sablin, E.P., Case, R.B., Shirleko, C.D., Hart, C.L., Ruby, A., Vale, R.D. and Fletterick, R.J.
(1998) Directionality determination in the minus-end-directed kinesin motor Ncd. Nature
395: 813-816.
97. Wells, S.E., Hillner, P.E., Vale, R.D. and Sachs, A.B. (1998) Circularization of mRNA by
eukaryotic translation initiation factors. Mol. Cell 2: 135-140.
98. Friedman, D. S. and Vale, R. D. (1999) Single molecule analysis of kinesin motility reveals
regulation by the cargo-binding tail domain. Nature Cell Biol. 1: 293-297.
99. Hartman, J. J. and Vale, R. D. (1999) A nucleotide-dependent oligomerization cycle by the
AAA enzyme katanin drives microtubule disassembly. Science 286: 782-785.
100. Pierce, D. W., Hom-Booher, N., Otsuka, A., and Vale, R.D. (1999) Single molecule behavior
of monomeric and heteromeric kinesins. Biochem. 38: 5412-5421.
101. Pierce, D.W. and Vale, R.D. (1999) Single-molecule fluorescence detection of green
fluorescence protein and application to single-protein dynamics. Meth. Cell Biol. 58: 49-73.
102. Pollock, N., de Hostos, E.L., Turck, C.W. and Vale, R.D. (1999) Reconstitution of membrane
transport powered by a novel dimeric kinesin motor of the K1F1A family purified from
Dictyostelium. J. Cell Biol. 147: 493-505.
103. Rice, S., Lin, A., W., Safer, D., Hart, C. L., Naber, N., Carragher, B. O., Cain, S. M.,
Pechatnikova, E., Wilson-Kubelek, E. M., Whitaker, M., Pate, E., Cooke, R., Taylor, E. W.,
Milligan, R. A. and Vale, R. D. (1999) A structural change in the kinesin motor protein that
drives motility. Nature 402: 778-784.
104. Sharp, D.J., McDonald, K.L., Brown, H.M., Matthies, H.J., Walczak, C., Vale, R.D.,
Mitchison, T.J. and Scholey, J.M. (1999) The bipolar kinesin, KLP61F, cross-links
microtubules within interpolar microtubule bundles of Drosophila embryonic mitotic
spindles. J. Cell Biol. 144: 125-138.
105. Vale, R. D. (1999) Millennial musings on molecular motors. Trends Cell Biol. 9: M38-M42.
106. Case, R.B., Rice, S., Hart, C., Ly, B. and Vale, R.D. (2000) Role of the kinesin neck linker and
catalytic core in microtubule-based motility. Curr. Biol. 10: 157-160.
107. Dell, K. R., Turck, C. W., and Vale, R. D. (2000) Mitotic phosphorylation of the dynein light
intermediate chain is mediated by cdc2 kinase. Traffic 1: 38-44.
108. Govindan, B. and Vale, R. D. (2000) Characterization of a microtubule assembly inhibitor
from Xenopus oocytes. Cell Motil. Cytoskel.: 45: 51-57.
109. Hopkins, S.C., Vale, R.D., and Kuntz, I.D. (2000) Inhibitors of kinesin activity from
structure-based computer screening. Biochemistry 39: 2805-2814.
110. Khan, S., Pierce, D. and Vale, R. D. (2000) Interactions of the chemotaxis signal protein
CheY with bacterial flagellar motors visualized by evanescent wave microscopy. Curr. Biol
10:. 927-930.
111. Klopfenstein, D., Vale, R.D. and Rogers, S. (2000) Motor protein receptors: moonlighting on
other jobs. Cell 103: 537-540.
112. Prahlad, V., Helfand, B.T., Langford, G. M., Vale, R. D. and Goldman, R. D. (2000) Fast
transport of neurofilament protein along microtubules in squid axoplasm. J. Cell Sci. 113:
3939-3946.
113. Shimizu, T., Thorn, K., Ruby, A. and Vale, R.D. (2000) ATPase kinetic characterization and
single molecule behavior of mutant human kinesin motors defective in microtubule-based
motility. Biochem. 37: 5265-5273.
114. Takizawa, P. A., DeRisi, J. L., Wilhelm, J. E. and Vale, R. D. (2000) mRNA localization and a
septin-based diffusion barrier create an asymmetric distribution of a yeast plasma
membrane protein. Science 290: 341-344.
115. Takizawa, P.A. and Vale, R.D. (2000) Role of the She proteins in Ash1 mRNA localization in
S. cerevisiae. Proc. Natl. Acad. Sci. U.S.A. 97: 5273-5278.
116. Thorn, K.S., Naber, N., Matuska, M., Vale, R.D. and Cooke, R. (2000) A novel method of
affinity-purifying proteins using a bis-arsenical fluorescein. Protein Sci. 9: 213-217.
117. Thorn, K., Ubersax, J.A., and Vale, R.D. (2000) Engineering the processive run length of the
kinesin motor. J. Cell Biol. 151: 1093-1100.
118. Tomishige, M. and Vale, R.D. (2000) Controlling kinesin by reversible disulfide cross-
linking: identifying the motility-producing conformational change. J. Cell Biol. 151: 1081-
1092.
119. Vale, R.D. (2000) AAA Proteins: Lords of the ring. J Cell Biol. 150: F13-F19.
120. Vale, R. D., Case, R., Sablin, E., Hart, C. and Fletterick, R. (2000) Searching for kinesin's
mechanical amplifier. Proc. Royal. Soc. London 355: 449-457.
 8

121. Vale, R. D. and Milligan, R. D. (2000). The way things move: looking under the hood of
molecular motors. Science 288: 88-95.
122. Wilhelm, J. E., Mansfield, J., Hom-Booher, N., Wang, S., Turck, C., Hazelrigg, T. and Vale,
R. D. (2000) Isolation of a ribonucleoprotein complex involved in mRNA localization in
Drosophila oocytes. J Cell Biol. 148: 427-439.
123. Wilhelm, J.E., Vale, R.D. and Hedge, R.S. (2000) Coordinate control of translation and
localization of Vg1 mRNA in Xenopus oocytes. Proc. Natl. Acad. Sci. 97: 13132-13137.
124. Irie, K., Tadauchi, T., Takizawa, P.A., Vale, R.D., Matsumoto, K. and Herskowitz, H. (2002)
The Khd1 protein, which has three KH RNA-binding motifs, is required for proper
localization of ASH1 mRNA in yeast. EMBO J. 21: 1158-1167.
125. Klopfenstein, D.T., Holleran, E.A. and Vale, R.D. (2002) Kinesin motors and microtubule-
based organelle transport in Dictyostelium discoideum. J. Mus. Res. Cell Motil. 23: 631-638.
126. Klopfenstein, D.R., Tomishige, M., Stuurman, N. and Vale, R.D. (2002) Role of
Phosphatidylinositol(4,5)bisphosphate Organization in Membrane Transport by the
Unc104 Kinesin Motor. Cell 109: 347-358.
127. Rogers, S.L., Rogers, G.C., Sharp, D.J. and Vale, R.D. (2002) Drosophila EB1 is important for
proper assembly, dynamics, and positioning of the mitotic spindle. J. Cell Biol. 158: 873-
884.
128. Tomishige, M., Klopfenstein, D.R. and Vale, R.D. (2002) Conversion of Unc104/KIF1A
kinesin into a processive motor after dimerization. Science 297: 2263-2267.
129. Al-Bassam, J., Cui, Y., Klopfenstein, D., Carragher, B.O., Vale, R.D. and Milligan, R.A.
(2003) Distinct conformations of the kinesin Unc104 neck regulate a monomer-to-dimer
motor transition, J. Cell Biol. 163: 743-753.
130. Goshima, G. and Vale, R.D. (2003) The roles of microtubule-based motor proteins in
mitosis: comprehensive RNAi analysis in the Drosophila S2 cell line. J Cell Biol. 162: 1003-
1016.
131. Naber N, Rice S, Matuska M, Vale RD, Cooke R. and Pate E. (2003) EPR spectroscopy
shows a microtubule-dependent conformational change in the kinesin switch 1 domain.
Biophys J. 84: 3190-6.
132. Naber N., Minehardt T.J., Rice S., Chen X., Grammer J., Matuska M., Vale R.D., Kollman
P.A., Car R., Yount R.G., Cooke R. and Pate E. (2003) Closing of the nucleotide pocket of
kinesin-family motors upon binding to microtubules. Science 300: 798-801.
133. Rice, S., Cui, Y., Sindelar, C., Naber, N., Matuska M., Vale, R. and Cooke R. (2003)
Thermodynamic properties of the kinesin neck region docking to the catalytic core.
Biophys. J. 84: 1844-1854.
134. Rogers, S.L., Weidemann, U., Stuurman, N. and Vale, R.D. (2003) Molecular requirements
for actin-based lamella formation in Drosophila S2 cells. J. Cell Biol. 162: 1079-1088.
135. Rothenberg, M.E., Rogers, S.L., Vale, R.D., Jan, L.Y. and Jan, Y.N. (2003) Drosophila Pod-1
crosslinks both actin and microtubules, and controls the targeting of axons. Neuron 39:
779-791.
136. Shepard, K.A., Gerber, A.P., Jambhekar, A., Takizawa, P.A., Brown, P.O., Herschlag, D.,
DeRisi, J.L. and Vale, R.D. (2003) Widespread cytoplasmic mRNA transport in S. cerevisiae:
Identification of 22 new bud-localized transcripts using DNA microarray analysis. Proc.
Natl. Acad. Sci. 100: 11429-11434.
137. Vale, R.D. (2003) Myosin V motor proteins: marching stepwise towards a mechanisms. J
Cell Biol 163: 445-450.
138. Vale, R.D. (2003) The molecular motor toolbox for intracellular transport. Cell 112: 467-480.
139. Yaffe, M.P., Stuurman, N. and Vale, R.D. (2003) Mitochondrial positioning in fission yeast
is driven by association with dynamic microtubules and mitotic spindle poles. Proc. Natl.
Acad. Sci. 100: 11424-11428.
140. Collins, S.R., Douglass, A., D. Vale, R.D. and Weissman, J.S. (2004) Mechanism of prion
propagation: amyloid growth occurs by monomer addition. PLOS Biology 2: 1582-1590.
141. Dell, K.R. and Vale, R.D. (2004) A tribute to Shinya Inoue and innovation in light
microscopy. J Cell Biol. 165: 21-5.
142. Klopfenstein, D.R. and Vale, R.D. (2004) The lipid binding pleckstrin homology in UNC-
104 kinesin is necessary for synaptic vesicle transport in the Caenorhabditis elegans. Mol.
Biol. Cell 15: 3729-3739.
143. Lawrence C.J., Dawe R.K., Christie K.R., Cleveland D.W., Dawson S.C., Endow S.A.,
Goldstein L.S., Goodson H.V., Hirokawa N., Howard J., Malmberg R.L., McIntosh J.R.,
Miki H., Mitchison T.J., Okada Y., Reddy A.S., Saxton W.M., Schliwa M., Scholey J.M., Vale
 9

R.D., Walczak C.E., Wordeman L. (2004) A standardized kinesin nomenclature. J Cell Biol.
167: 19-22.
144. Reck-Peterson, S.L. and Vale, R.D. (2004) Molecular dissection of the roles of nucleotide
binding and hydrolysis in dynein's AAA domains in S. cerevisiae. Proc. Natl. Acad. Sci. 101:
1491-1495.
145. Rogers, G.C., Rogers, S.L., Schwimmer, T.A., Ems-McClung, S.C., Walczak, C.E., Vale, R.D.,
Scholey, J.M. and Sharp, D.J. (2004) Two mitotic kinesins cooperate to drive sister
chromatid separation during anaphase. Nature 427: 364-370.
146. Yildiz, A., Tomishige, M., Vale, R.D. and Selvin, P.R. (2004) Kinesin walks hand-over-hand.
Science 303: 676-678.
147. Rogers, S.L., Wiedemann, U., Haecker, U. and Vale, R.D. (2004) Drosophila DRhoGEF2
associates with microtubule plus ends in an EB1-dependent manner. Curr. Biol. 14: 1827-
1833.
148. Cheng, L.W., Viala, J.P.M., Stuurman, N., Wiedemann, U., Vale, R.D., Portnoy, D.A. (2005)
Use of RNA interference in Drosophila S2 cells to identify host pathways controlling
compartmentalization of an intracellular pathogen. Proc. Natl. Acad. Sci. 102: 13646-
13651.
149. Dean, S.O., Rogers, S.L., Stuurman, N., Vale, R.D., Spudich, J.A. (2005) Distinct pathways
control recruitment and maintenance of myosin II at the cleavage furrow during
cytokinesis. Proc. Natl. Acad. Sci. 38: 13473-13478.
150. Douglass, A.D. and Vale R.D. (2005). Single molecule imaging in living cells by total
internal reflection fluorescence microscopy. In: Cell Biology: A Laboratory Handbook,
3rd ed., Elsevier Sci (USA), pp. 129-136.
151. Douglass, A. and Vale, R.D. (2005) Single molecule microscopy reveals plasma membrane
microdomains created by protein-protein networks that exclude or trap signaling
molecules in T cells. Cell 121: 937-950.
152. Dzhindzhev N.S., Rogers S.L., Vale R.D., Ohkura H. (2005) Distinct mechanisms govern the
localisation of Drosophila CLIP-190 to unattached kinetochores and microtubule plus-ends.
J Cell Sci. 118: 3781-90.
153. Gibbons, I.R., Garbarino, J.E., Tan, C.E., Reck-Peterson, S.L., Vale, R.D., and Carter, A.P.
(2005) The affinity of the dynein microtubule-binding domain is modulated by the
conformation of its coiled-coil stalk. J. Biol. Chem. 280: 23960-23965.
154. Goshima, G., Nédélec, F., and Vale, R.D. (2005) Mechanisms for focusing mitotic spindle
poles by minus-end-directed motor proteins. J. Cell Biol. 171: 229-240.
155. Goshima, G. and Vale, R.D. (2005) Cell Cycle-dependent Dynamics and Regulation of
Mitotic Kinesins in Drosophila S2 Cells Mol. Biol. Cell 16: 3896-3907.
156. Goshima, G., Wollman, R., Stuurman, N., Scholey, J.M., Vale, R.D. (2005) Length control of
the metaphase spindle. Curr Biol. 15: 1979-1988.
157. Jambhekar, A., McDermott, K., Sorber, K, Shepard, K.A., Vale, R.D., Takizawa, P.A.,
DeRisi, J.L. (2005) Unbiased selection of localization elements reveals cis-acting
determinants of mRNA bud-localization in Saccharomyces cerevisiae. Proc. Natl. Acad. Sci.
102: 18005-18010.
158. Mennella, V., Rogers, G.C., Rogers, S.L., Buster, D.W., Vale, R.D. and Sharp, D.J. (2005)
Functionally distinct kinesin-13 family members cooperate to regulate microtubule
dynamics during interphase. Nat. Cell Biol. 7: 235-245.
159. Roll-Mecak, A. and Vale, R.D. (2005) The Drosophila homologue of the hereditary spastic
paraplegia protein, spastin, severs and disassembles microtubules. Curr. Biol. 15: 650-655.
160. Slep, K.C., Rogers, S.L., Elliott, S.L., Ohkura, H., Kolodziej, P.A. and Vale, R.D. (2005)
Structural determinants for EB1-mediated recruitment of APC and spectraplakins to the
microtubule plus end. J. Cell Biol. 168: 587-598.
161. Endres, N.F., Yoshioka, C., Milligan, R.A. and R. D. Vale. (2006) A lever arm rotation drives
motility of the minus-end-directed kinesin, Ncd. Nature 439: 875-878.
162. Imanishi, M., Endres, N.F., Gennerich, A. and Vale, R.D. (2006) Auto-Inhibition Regulates
the Motility of the C. elegans Intraflagellar Transport Motor, OSM-3. J. Cell Biol. 174: 931-
937.
163. Mahoney, N.M., Goshima, G., Douglass, A., Vale, R.d. (2006) Making microtubules and
mitotic spindles in cells without functional centrosomes. Curr. Biol. 16: 564-569.
164. Pan, X., Ou, G., Civelekoglu-Scholey, G., Blacque, O.E., Endres, N.F., Tao, L., Mogilner, A.,
Leroux, M.R., Vale, R.D. and Scholey, J.M. (2007) Mechanism of transport of IFT-particles of
kinesin-II and OSM-3 motors. J. Cell Biol. 174: 1035-1045.
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165. Reck-Peterson, S.L., Yildiz, A., Carter, A.P., Gennerich, A., Zhang, N., and Vale, R.D. (2006)
Stepping behavior and structural requirements for dynein processivity. Cell 126: 335-348.
166. Roll-Mecak, A. and Vale, R.D. (2006) Making more microtubules by severing: a common
theme of noncentrosomal microtubule arrays? J. Cell Biol. 175: 849-851.
167. Tomishige, M., Stuurman, N. and Vale, R. D. (2006) Single molecule observations of the
neck linker conformational changes in the kinesin motor protein. Nat. Struct. Mol. Biol.
13: 887-894.
168. Dustin, M.L., Starr, T., Coombs, D., Majeau, G.R., Meier, W., Hochman, P.S., Douglass, A.,
Vale, R.D., Goldstein, B. and Whitty, A. (2007) Quantification and modeling of tripartite
CD2-, CD58FC Chimera (Alefacept)-, and CD16-mediated Cell Adhesion. J Biol Chem 282:
34748-34757.
169. Gennerich, A., Carter, A.P., Reck-Peterson, S.L. and Vale, R.D. (2007) Force-induced
bidirectional stepping of cytoplasmic dynein. Cell 131: 952-965.
170. Goshima, G., Wollman, R., Goodwin, S., Zhang, N., Scholey, J.M., Vale, R.D. and Stuurman,
N. (2007) Genes required for mitotic spindle assembly in Drosophila S2 cells. Science 316:
417-421.
171. Griffis, E.R., Stuurman, N. and Vale, R.D. (2007) Spindly, a novel protein essential for
silencing the spindle assembly checkpoint, recruits dynein to the kinetochore, J. Cell Biol.
177: 1005-1015.
172. Kaizuka, Y., Douglass, A.,Varma, R., Dustin, M. and Vale, R.D. (2007) Mechanisms for
segregating T cell receptor and adhesion molecules during immunological synapse
formation in Jurkat T cell, Proc. Natl. Acad. Sci. 104: 20296-20301.
173. Mori, T., Vale, R.D. and Tomishige, M. (2007) How kinesin waits between steps. Nature
450: 750-755.
174. Slep, K.C. and Vale, R.D. (2007) Structural Basis of Microtubule Plus End Tracking by
XMAP215, Clip-170 and EB1. Mol. Cell 27: 976-991.
175. Stuurman, N., Amodaj, N and Vale, R.D. (2007) µManager: Open Source software for light
microscope imaging. Microscopy Today 15: 42-43.
176. Cho, C., Reck-Peterson, S.L., Vale, R.D. (2008) Regulatory ATPase Sites of cytoplasmic
dynein affect processivity and force generation. J. Biol. Chem. 283: 25839-25845.
177. Douglass, A. and Vale, R.D. (2008) Single-molecule imaging of fluorescent proteins.
Methods Cell Biol. 85: 113-125.
178. Goshima G. , Mayer M. , Zhang N. , Stuurman N. , and Vale R.D. (2008). Augmin: a protein
complex required for centrosome-independent microtubule generation within the spindle.
J Cell Biol. 181: 421-429.
179. Guo, Y., Walther, T.C., Rao, M., Stuurman, N., Goshima, G., Terayama, K., Wong, J.S., Vale,
R.D., Walter, P., Farese, R.V. (2008) Functional genomic screen reveals genes involved in
lipid droplet formation and utilization. Nature 453: 657-61.
180. Roll-Mecak, A. and Vale, R.D. (2008) Structural basis of microtubule severing by the
hereditary spastic paraplegia protein spastin. Nature 451: 363 – 367.
181. Trammel, M.A., Mahoney, N.M., Agard, D.A. and Vale, R.D. (2008) Mob4 Plays a role in
spindle focusing in Drosophila S2 cells. J. Cell Sci. 121: 1284-1292.
182. Vale, R.D. (2008) Microscopes for fluorimeters: The era of single molecule measurements.
Cell 135: 779-785.
183. Yildiz, A., Tomishige, M., Gennerich, A. and Vale, R.D. (2008) Intramolecular strain
coordinates kinesin stepping behavior along microtubules. Cell 134: 1030-1041.
184. Carter, A., Garbarino, J.E., Wilson-Kubalek, E.M., Shipley, W.E., Cho, C., Milligan, R.A.,
Vale, R.D., Gibbons, I.R. Structure and functional role of dynein's microtubule binding
domain. Science, in press.
185. Gennerich, A. and R. D. Vale. Walking the walk: How kinesin and dynein coordinate their
steps. Current Opinion in Cell Biology, vol. 21, in press.
186. Kaizuka, Y., Douglass, A.D., Vardhana, S., Dustin, M.L. and Vale, R.D. Two distinct T cell
receptors, TCR and CD2, transduce signals by condensing similar signaling molecules into
plasma membrane microdomains, JCB, in revision.
186. Kardon, J.R., Reck-Peterson, S.L. and Vale, R.D. Regulation of the processivity and
intracellular localization of S. cerevisiae dynein by dynactin, PNAS, in press.