CHAPTER 3 - MOVEMENT OF SUBSTANCES ACROSS THE PLASMA MEMBRANE Topic :Determining the concentration of an external solution which is isotonic

to the cell sap of a plant Objective /Aim: Determining the concentration of sucrose solution which is isotonic to the cell sap of potato cylinder Problem Statement: What is the concentration of the sucrose solution that will maintain the mass of potato cylinder? Hypothesis: As the sucrose solution reach certain concentration (where it is isotonic to cell sap) ,there is no change in the mass of potato cylinder. Variables : manipulated responding controlled Apparatus : Cork borer ,test tube/specimen tube, stop watch, ruler, knife Materials : Potatoes, various concentration of sucrose solution, filter paper Technique : Measure and record the final mass of potato cylinder by using electronic balance Procedure : 1. Seven test tubes are labeled P,Q,R,S,T ,U and V. 2. Test tube P is filled with 25 ml distilled water, test tube Q is filled with 25ml sucrose solution 0.1 M, test tube R is filled with 25ml sucrose solution 0.2M, test tube S is filled with 25 ml sucrose solution 0.3M, test tube T is filled with 25 ml sucrose solution 0.4M , test tube U is filled with 25 ml sucrose solution 0.5M and test tube V is filled with 25ml sucrose solution 0.6M 3. The cork borer is pushed into the potato and the potato cylinder is obtained by pushing it out of the cork borer using glass rod . 4. The potato cylinders are cut to the exact length of 5.0 cm. 5. Record the initial mass of potato cylinders by using electronic balance. 6. One potato cylinder is placed in each labeled test tube for 30 minutes. 7. After 30 minutes, the potato cylinders are removed from the test tube and gently wiped with filter paper . 8. The final mass of the potato cylinders are measured. 9. The final mass of the potato cylinders are recorded in a result table. : the concentration of the sucrose solution : change in mass of potato cylinder : type of potato // volume of solution //immersing time

10. A graph of the concentration of sucrose solution against the change in mass is plotted. Result: Test Concentration tube of sucrose /Spe solution cime (M) n tube P Distilled water Q 0.1M R S T U V 0.2M 0.3M 0.4M 0.5M 0.6M Texture

Mass
Initial (g) Final (g) Change in mass (g)

Discussion: Answer the 4 questions (practical book page 30) 1. The potato strips in the test tube with distilled water and 0.1M sucrose to 0.3M sucrose is where as in 0.5m sucrose to 0.6M sucrose is.. 2. The x-axis represents the.. While the y-axis represents the 3. The point where the graph cuts the x-axis indicates that there is .This mean that the concentration of sucrose solution at this point isto the cell sap of the plant tissue. 4. If the solution is hypotonic to concentration of cell sap, then the water..by osmosis. If the solution is hypertonic to concentration of cell sap, then the water..by osmosis. If the solution is isotonic to concentration of cell sap, then the flow of water.. Conclusion :

The concentration of external solution which is isotonic to cell sap of potato cell is ..whereby the mass of the potato strip remains the same. The hypothesis is accepted.