References for Phytoestrogens in menopause Aust J Medical Herbalism 23:1;9-15 March 2011 (author format)

Abascal, K., & Yarnell, E. 2007. Kudzu - The miracle vine, Alternative & complementary therapies, 13: 78-85. Adlercreutz, H. 2002. Phyto-oestrogens and cancer. Lancet Oncol, 3;6: 364-373. Adlercreutz, H., Fotsis, T., Lampe, J., Wahala, K., Makela, T., Brunow, G., & Hase, T. 1993. Quantitative determination of lignans and isoflavonoids in plasma of omnivorous and vegetarian women by isotope dilution gas chromatography-mass spectrometry. Scand J Clin Lab Invest Suppl, 215: 5-18. Adlercreutz, H., Hockerstedt, K., Bannwart, C., Bloigu, S., Hamalainen, E., Fotsis, T., & Ollus, A. 1987. Effect of dietary components, including lignans and phytoestrogens, on enterohepatic circulation and liver metabolism of estrogens and on sex hormone binding globulin ;SHBG. J Steroid Biochem, 27;4-6: 1135-1144. Adlercreutz, H., Mousavi, Y., Clark, J., Hockerstedt, K., Hamalainen, E., Wahala, K., Makela, T., & Hase, T. 1992. Dietary phytoestrogens and cancer: in vitro and in vivo studies. J Steroid Biochem Mol Biol, 41;3-8: 331-337. Albertazzi, P., Pansini, F., Bonaccorsi, G., Zanotti, L., Forini, E., & de Aloysio, D. 1998. The effect of dietary soy supplementation on hot flushes. Obstetrics and Gynecology, 91;6: 6-11. Allred, C. D., Allred, K. F., Ju, Y. H., Goeppinger, T. S., Doerge, D. R., & Helferich, W. G. 2004. Soy processing influences growth of estrogen-dependent breast cancer tumors. Carcinogenesis, 25;9: 1649-1657. Allred, C. D., Allred, K. F., Ju, Y. H., Virant, S. M., & Helferich, W. G. 2001. Soy diets containing varying amounts of genistein stimulate growth of estrogen-dependent ;MCF-7 tumors in a dose-dependent manner. Cancer Res, 61;13: 5045-5050. Allred, C. D., Twaddle, N. C., Allred, K. F., Goeppinger, T. S., Churchwell, M. I., Ju, Y. H., Helferich, W. G., & Doerge, D. R. 2005. Soy processing affects metabolism and disposition of dietary isoflavones in ovariectomized BALB/c mice. J Agric Food Chem, 53;22: 8542-8550. Anderson, J. W., Johnstone, B. M., & Cook-Newell, M. E. 1995. Meta-analysis of the effects of soy protein intake on serum lipids. N Engl J Med, 333;5: 276-282. Atkinson, C., Compston, J. E., Day, N. E., Dowsett, M., & Bingham, S. A. 2004. The effects of phytoestrogen isoflavones on bone density in women: a double-blind, randomized, placebocontrolled trial. Am J Clin Nutr, 79;2: 326-333. Baber, R. J., Templeman, C., Morton, T., Kelly, G. E., & West, L. 1999. Randomized placebocontrolled trial of an isoflavone supplement and menopausal symptoms in women. Climacteric, 2: 85-92. Balk, J. L., Whiteside, D. A., Naus, G., DeFerrari, E., & Roberts, J. M. 2002. A pilot study of the effects of phytoestrogen supplementation on postmenopausal endometrium. J Soc Gynecol Investig, 9;4: 238-242. Barnes, S., Grubbs, C., Setchell, K. D., & Carlson, J. 1990. Soybeans inhibit mammary tumors in models of breast cancer. Prog Clin Biol Res, 347: 239-253. Basaria, S., Wisniewski, A., Dupree, K., Bruno, T., Song, M. Y., Yao, F., Ojumu, A., John, M., & Dobs, A. S. 2009. Effect of high-dose isoflavones on cognition, quality of life, androgens, and lipoprotein in post-menopausal women. J Endocrinol Invest, 32;2: 150-155. Beck, V., Rohr, U., & Jungbauer, A. 2005. Phytoestrogens derived from red clover: an alternative to estrogen replacement therapy? J Steroid Biochem Mol Biol, 94;5: 499-518. Bodinet, C., & Freudenstein, J. 2004. Influence of marketed herbal menopause preparations on MCF7 cell proliferation. Menopause, 11;3: 281-289. Booth, N. L., Overk, C. R., Yao, P., Burdette, J. E., Nikolic, D., Chen, S. N., Bolton, J. L., van Breemen, R. B., Pauli, G. F., & Farnsworth, N. R. 2006a. The chemical and biologic profile of a red clover ;Trifolium pratense L. phase II clinical extract. J Altern Complement Med, 12;2: 133-139. Booth, N. L., Piersen, C. E., Banuvar, S., Geller, S. E., Shulman, L. P., & Farnsworth, N. R. 2006b. Clinical studies of red clover ;Trifolium pratense dietary supplements in menopause: a literature review. Menopause, 13;2: 251-264.

Boyapati, S. M., Shu, X. O., Ruan, Z. X., Dai, Q., Cai, Q., Gao, Y. T., & Zheng, W. 2005. Soyfood intake and breast cancer survival: a followup of the Shanghai Breast Cancer Study. Breast Cancer Res Treat, 92;1: 11-17. Burdette, J. E., Liu, J., Lantvit, D., Lim, E., Booth, N., Bhat, K. P., Hedayat, S., Van Breemen, R. B., Constantinou, A. I., Pezzuto, J. M., Farnsworth, N. R., & Bolton, J. L. 2002. Trifolium pratense ;red clover exhibits estrogenic effects in vivo in ovariectomized Sprague-Dawley rats. J Nutr, 132;1: 27-30. Burke, G. L., Legault, C., Anthony, M., Bland, D. R., Morgan, T. M., Naughton, M. J., Leggett, K., Washburn, S. A., & Vitolins, M. Z. 2003. Soy protein and isoflavone effects on vasomotor symptoms in peri- and postmenopausal women: the Soy Estrogen Alternative Study. Menopause, 10;2: 147-153. Cabanes, A., Wang, M., Olivo, S., DeAssis, S., Gustafsson, J. A., Khan, G., & Hilakivi-Clarke, L. 2004. Prepubertal estradiol and genistein exposures up-regulate BRCA1 mRNA and reduce mammary tumorigenesis. Carcinogenesis, 25;5: 741-748. Cassidy, A., & Milligan, S. 1998. How significant are environmental estrogens to women? Climacteric, 1;3: 229-242. Chang, W. H., Liu, J. J., Chen, C. H., Huang, T. S., & Lu, F. J. 2002. Growth inhibition and induction of apoptosis in MCF-7 breast cancer cells by fermented soy milk. Nutr Cancer, 43;2: 214-226. Chen, W. F., Huang, M. H., Tzang, C. H., Yang, M., & Wong, M. S. 2003. Inhibitory actions of genistein in human breast cancer ;MCF-7 cells. Biochim Biophys Acta, 1638;2: 187-196. Chen, W. F., & Wong, M. S. 2004. Genistein enhances insulin-like growth factor signaling pathway in human breast cancer ;MCF-7 cells. J Clin Endocrinol Metab, 89;5: 2351-2359. Cheng, G., Wilczek, B., Warner, M., Gustafsson, J. A., & Landgren, B. M. 2007. Isoflavone treatment for acute menopausal symptoms. Menopause, 14;3 Pt 1: 468-473. Cherdshewasart, W., & Sutjit, W. 2008. Correlation of antioxidant activity and major isoflavonoid contents of the phytoestrogen-rich Pueraria mirifica and Pueraria lobata tubers. Phytomedicine, 15;1-2: 38-43. Clifton-Bligh, P. B., Baber, R. J., Fulcher, G. R., Nery, M. L., & Moreton, T. 2001. The effect of isoflavones extracted from red clover ;Rimostil on lipid and bone metabolism. Menopause, 8;4: 259-265. Collins-Burow, B. M., Burow, M. E., Duong, B. N., & McLachlan, J. A. 2000. Estrogenic and antiestrogenic activities of flavonoid phytochemicals through estrogen receptor bindingdependent and -independent mechanisms. Nutr Cancer, 38;2: 229-244. Crisafulli, A., Marini, H., Bitto, A., Altavilla, D., Squadrito, G., Romeo, A., Adamo, E. B., Marini, R., D'Anna, R., Corrado, F., Bartolone, S., Frisina, N., & Squadrito, F. 2004. Effects of genistein on hot flushes in early postmenopausal women: a randomized, double-blind EPT- and placebo-controlled study. Menopause, 11;4: 400-404. D'Anna, R., Cannata, M. L., Atteritano, M., Cancellieri, F., Corrado, F., Baviera, G., Triolo, O., Antico, F., Gaudio, A., Frisina, N., Bitto, A., Polito, F., Minutoli, L., Altavilla, D., Marini, H., & Squadrito, F. 2007. Effects of the phytoestrogen genistein on hot flushes, endometrium, and vaginal epithelium in postmenopausal women: a 1-year randomized, double-blind, placebocontrolled study. Menopause, 14;4: 648-655. Dai, Q., Franke, A. A., Jin, F., Shu, X. O., Hebert, J. R., Custer, L. J., Cheng, J., Gao, Y. T., & Zheng, W. 2002. Urinary excretion of phytoestrogens and risk of breast cancer among Chinese women in Shanghai. Cancer Epidemiol Biomarkers Prev, 11;9: 815-821. Dai, Q., Franke, A. A., Yu, H., Shu, X. O., Jin, F., Hebert, J. R., Custer, L. J., Gao, Y. T., & Zheng, W. 2003. Urinary phytoestrogen excretion and breast cancer risk: evaluating potential effect modifiers endogenous estrogens and anthropometrics. Cancer Epidemiol Biomarkers Prev, 12;6: 497-502. Dai, Q., Shu, X. O., Jin, F., Potter, J. D., Kushi, L. H., Teas, J., Gao, Y. T., & Zheng, W. 2001. Population-based case-control study of soyfood intake and breast cancer risk in Shanghai. Br J Cancer, 85;3: 372-378. Dalais, F. S., Rice, G. E., Wahlqvist, M. L., Grehan, M., Murkies, A. L., Medley, G., Ayton, R., & Strauss, B. J. 1998. Effects of dietary phytoestrogens in postmenopausal women. Climacteric, 1;2: 124-129. Dodin, S., Lemay, A., Jacques, H., Legare, F., Forest, J. C., & Masse, B. 2005. The effects of flaxseed dietary supplement on lipid profile, bone mineral density, and symptoms in menopausal women: a randomized, double-blind, wheat germ placebo-controlled clinical trial. J Clin Endocrinol Metab, 90;3: 1390-1397.

Doerge, D. R., & Sheehan, D. M. 2002. Goitrogenic and estrogenic activity of soy isoflavones. Environ Health Perspect, 110 Suppl 3: 349-353. Erkkola, R., Vervarcke, S., Vansteelandt, S., Rompotti, P., De Keukeleire, D., & Heyerick, A. 2010. A randomized, double-blind, placebo-controlled, cross-over pilot study on the use of a standardized hop extract to alleviate menopausal discomforts. Phytomedicine, 17;6: 389-396. Faure, E. D., Chantre, P., & Mares, P. 2002. Effects of a standardized soy extract on hot flushes: a multicenter, double-blind, randomized, placebo-controlled study. Menopause, 9;5: 329-334. Ferrari, A. 2009. Soy extract phytoestrogens with high dose of isoflavones for menopausal symptoms. J Obstet Gynaecol Res, 35;6: 1083-1090. Fink, B. N., Steck, S. E., Wolff, M. S., Britton, J. A., Kabat, G. C., Gaudet, M. M., Abrahamson, P. E., Bell, P., Schroeder, J. C., Teitelbaum, S. L., Neugut, A. I., & Gammon, M. D. 2007. Dietary flavonoid intake and breast cancer survival among women on Long Island. Cancer Epidemiol Biomarkers Prev, 16;11: 2285-2292. Fioravanti, L., Cappelletti, V., Miodini, P., Ronchi, E., Brivio, M., & Di Fronzo, G. 1998. Genistein in the control of breast cancer cell growth: insights into the mechanism of action in vitro. Cancer Lett, 130;1-2: 143-152. Folman, Y., & Pope, G. S. 1966. The interaction in the immature mouse of potent oestrogens with coumestrol, genistein and other utero-vaginotrophic compounds of low potency. J Endocrinol, 34;2: 215-225. Fugh-Berman, A., & Kronenberg, F. 2001. Red clover ;Trifolium pratense for menopausal women: current state of knowledge. Menopause, 8;5: 333-337. Geller, S. E., Shulman, L. P., van Breemen, R. B., Banuvar, S., Zhou, Y., Epstein, G., Hedayat, S., Nikolic, D., Krause, E. C., Piersen, C. E., Bolton, J. L., Pauli, G. F., & Farnsworth, N. R. 2009. Safety and efficacy of black cohosh and red clover for the management of vasomotor symptoms: a randomized controlled trial. Menopause, 16;6: 1156-1166. Goetz, P. 2007. The role of hops and its components in the treatment of menopause. Phytotherapie, 5;2: 83-85. Gotoh, T., Yamada, K., Ito, A., Yin, H., Kataoka, T., & Dohi, K. 1998. Chemoprevention of N-nitrosoN-methylurea-induced rat mammary cancer by miso and tamoxifen, alone and in combination. Jpn J Cancer Res, 89;5: 487-495. Gu, L., House, S. E., Prior, R. L., Fang, N., Ronis, M. J., Clarkson, T. B., Wilson, M. E., & Badger, T. M. 2006. Metabolic phenotype of isoflavones differ among female rats, pigs, monkeys, and women. J Nutr, 136;5: 1215-1221. Hakimi, S. 2006. Probable effects of fenugreek seed on hot flash in menopausal women. Journal of Medicinal Plants, 5;19: 9-14+65. Han, D., Tachibana, H., & Yamada, K. 2001. Inhibition of environmental estrogen-induced proliferation of human breast carcinoma MCF-7 cells by flavonoids. In Vitro Cell Dev Biol Anim, 37;5: 275-282. Han, K. K., Soares, J. M., Jr., Haidar, M. A., de Lima, G. R., & Baracat, E. C. 2002. Benefits of soy isoflavone therapeutic regimen on menopausal symptoms. Obstet Gynecol, 99;3: 389-394. Hargreaves, D. F., Potten, C. S., Harding, C., Shaw, L. E., Morton, M. S., Roberts, S. A., Howell, A., & Bundred, N. J. 1999. Two-week dietary soy supplementation has an estrogenic effect on normal premenopausal breast. J Clin Endocrinol Metab, 84;11: 4017-4024. Heyerick, A., Vervarcke, S., Depypere, H., Bracke, M., & De Keukeleire, D. 2006. A first prospective, randomized, double-blind, placebo-controlled study on the use of a standardized hop extract to alleviate menopausal discomforts. Maturitas, 54;2: 164-175. Hillerns, P. I., Zu, Y., Fu, Y. J., & Wink, M. 2005. Binding of phytoestrogens to rat uterine estrogen receptors and human sex hormone-binding globulins. Z Naturforsch [C], 60;7-8: 649-656. Hirose, K., Tajima, K., Hamajima, N., Inoue, M., Takezaki, T., Kuroishi, T., Yoshida, M., & Tokudome, S. 1995. A large-scale, hospital-based case-control study of risk factors of breast cancer according to menopausal status. Jpn J Cancer Res, 86;2: 146-154. Ho, S. C., Woo, J., Lam, S., Chen, Y., Sham, A., & Lau, J. 2003. Soy protein consumption and bone mass in early postmenopausal Chinese women. Osteoporos Int, 14;10: 835-842. Hodgert Jury, H., Zacharewski, T. R., & Hammond, G. L. 2000. Interactions between human plasma sex hormone-binding globulin and xenobiotic ligands. J Steroid Biochem Mol Biol, 75;2-3: 167-176. Hsieh, C. Y., Santell, R. C., Haslam, S. Z., & Helferich, W. G. 1998. Estrogenic effects of genistein on the growth of estrogen receptor-positive human breast cancer ;MCF-7 cells in vitro and in vivo. Cancer Res, 58;17: 3833-3838.

Ikeda, T., Nishikawa, A., Imazawa, T., Kimura, S., & Hirose, M. 2000. Dramatic synergism between excess soybean intake and iodine deficiency on the development of rat thyroid hyperplasia. Carcinogenesis, 21;4: 707-713. Jefferson, W. N., & Newbold, R. R. 2000. Potential endocrine-modulating effects of various phytoestrogens in the diet. Nutrition, 16;7-8: 658-662. Ju, Y. H., Allred, C. D., Allred, K. F., Karko, K. L., Doerge, D. R., & Helferich, W. G. 2001. Physiological concentrations of dietary genistein dose-dependently stimulate growth of estrogen-dependent human breast cancer ;MCF-7 tumors implanted in athymic nude mice. J Nutr, 131;11: 2957-2962. Ju, Y. H., Doerge, D. R., & Helferich, W. G. 2008. A dietary supplement for female sexual dysfunction, Avlimil, stimulates the growth of estrogen-dependent breast tumors; MCF-7 implanted in ovariectomized athymic nude mice. Food Chem Toxicol, 46;1: 310-320. Ju, Y. H., Fultz, J., Allred, K. F., Doerge, D. R., & Helferich, W. G. 2006. Effects of dietary daidzein and its metabolite, equol, at physiological concentrations on the growth of estrogendependent human breast cancer ;MCF-7 tumors implanted in ovariectomized athymic mice. Carcinogenesis, 27;4: 856-863. Kaldas, R. S., & Hughes, C. L., Jr. 1989. Reproductive and general metabolic effects of phytoestrogens in mammals. Reprod Toxicol, 3;2: 81-89. Kang, S. C., Lee, C. M., Choi, H., Lee, J. H., Oh, J. S., Kwak, J. H., & Zee, O. P. 2006. Evaluation of oriental medicinal herbs for estrogenic and antiproliferative activities. Phytother Res, 20;11: 1017-1019. Kao, Y. C., Zhou, C., Sherman, M., Laughton, C. A., & Chen, S. 1998. Molecular basis of the inhibition of human aromatase ;estrogen synthetase by flavone and isoflavone phytoestrogens: A site-directed mutagenesis study. Environ Health Perspect, 106;2: 85-92. Khaodhiar, L., Ricciotti, H. A., Li, L., Pan, W., Schickel, M., Zhou, J., & Blackburn, G. L. 2008. Daidzein-rich isoflavone aglycones are potentially effective in reducing hot flashes in menopausal women. Menopause, 15;1: 125-132. Kimura, S., Suwa, J., Ito, M., & Sato, H. 1976. Development of malignant goiter by defatted soybean with iodine-free diet in rats. Gann, 67;5: 763-765. Knight, D. C., Howes, J. B., & Eden, J. 1999. The effect of PromensilTM, an isoflavone extract, on menopausal symptoms. Climacteric, 2: 79-84. Knight, D. C., Howes, J. B., Eden, J. A., & Howes, L. G. 2001. Effects on menopausal symptoms and acceptability of isoflavone-containing soy powder dietary supplementation. Climacteric, 4;1: 13-18. Kotsopoulos, D., Dalais, F. S., Liang, Y. L., McGrath, B. P., & Teede, H. J. 2000. The effects of soy protein containing phytoestrogens on menopausal symptoms in postmenopausal women. Climacteric, 3;3: 161-167. Kritz-Silverstein, D., & Goodman-Gruen, D. L. 2002. Usual dietary isoflavone intake, bone mineral density, and bone metabolism in postmenopausal women. J Womens Health Gend Based Med, 11;1: 69-78. Kuiper, G. G., Lemmen, J. G., Carlsson, B., Corton, J. C., Safe, S. H., van der Saag, P. T., van der Burg, B., & Gustafsson, J. A. 1998. Interaction of estrogenic chemicals and phytoestrogens with estrogen receptor beta. Endocrinology, 139;10: 4252-4263. Lamlertkittikul, S., & Chandeying, V. 2004. Efficacy and safety of Pueraria mirifica ;Kwao Kruea Khao for the treatment of vasomotor symptoms in perimenopausal women: Phase II Study. J Med Assoc Thai, 87;1: 33-40. Lapcik, O., Hampl, R., Hill, M., Wahala, K., Maharik, N. A., & Adlercreutz, H. 1998. Radioimmunoassay of free genistein in human serum. J Steroid Biochem Mol Biol, 64;5-6: 261-268. Lee, H. P., Gourley, L., Duffy, S. W., Esteve, J., Lee, J., & Day, N. E. 1991. Dietary effects on breastcancer risk in Singapore. Lancet, 337;8751: 1197-1200. Lee, H. P., Gourley, L., Duffy, S. W., Esteve, J., Lee, J., & Day, N. E. 1992. Risk factors for breast cancer by age and menopausal status: a case-control study in Singapore. Cancer Causes Control, 3;4: 313-322. Lemay, A., Dodin, S., Kadri, N., Jacques, H., & Forest, J. C. 2002. Flaxseed dietary supplement versus hormone replacement therapy in hypercholesterolemic menopausal women. Obstet Gynecol, 100;3: 495-504. Liu, J., Burdette, J. E., Xu, H., Gu, C., van Breemen, R. B., Bhat, K. P., Booth, N., Constantinou, A. I., Pezzuto, J. M., Fong, H. H., Farnsworth, N. R., & Bolton, J. L. 2001. Evaluation of estrogenic

activity of plant extracts for the potential treatment of menopausal symptoms. J Agric Food Chem, 49;5: 2472-2479. Lucas, E. A., Wild, R. D., Hammond, L. J., Khalil, D. A., Juma, S., Daggy, B. P., Stoecker, B. J., & Arjmandi, B. H. 2002. Flaxseed improves lipid profile without altering biomarkers of bone metabolism in postmenopausal women. J Clin Endocrinol Metab, 87;4: 1527-1532. Lukaczer, D., Darland, G., Tripp, M., Liska, D., Lerman, R. H., Schiltz, B., & Bland, J. S. 2005. Clinical effects of a proprietary combination isoflavone nutritional supplement in menopausal women: a pilot trial. Altern Ther Health Med, 11;5: 60-65. Mai, Z., Blackburn, G. L., & Zhou, J. R. 2007. Soy phytochemicals synergistically enhance the preventive effect of tamoxifen on the growth of estrogen-dependent human breast carcinoma in mice. Carcinogenesis, 28;6: 1217-1223. Maki, P. M., Rubin, L. H., Fornelli, D., Drogos, L., Banuvar, S., Shulman, L. P., & Geller, S. E. 2009. Effects of botanicals and combined hormone therapy on cognition in postmenopausal women. Menopause, 16;6: 1167-1177. Manonai, J., Chittacharoen, A., Udomsubpayakul, U., Theppisai, H., & Theppisai, U. 2008. Effects and safety of Pueraria mirifica on lipid profiles and biochemical markers of bone turnover rates in healthy postmenopausal women. Menopause, 15;3: 530-535. Martinez-Montemayor, M. M., Otero-Franqui, E., Martinez, J., De La Mota-Peynado, A., Cubano, L. A., & Dharmawardhane, S. 2010. Individual and combined soy isoflavones exert differential effects on metastatic cancer progression. Clin Exp Metastasis, 27;7: 465-480. Maskarinec, G., Takata, Y., Franke, A. A., Williams, A. E., & Murphy, S. P. 2004. A 2-year soy intervention in premenopausal women does not change mammographic densities. J Nutr, 134;11: 3089-3094. Maskarinec, G., Williams, A. E., & Carlin, L. 2003. Mammographic densities in a one-year isoflavone intervention. Eur J Cancer Prev, 12;2: 165-169. Mathey, J., Lamothe, V., Coxam, V., Potier, M., Sauvant, P., & Pelissero, C. B. 2006. Concentrations of isoflavones in plasma and urine of post-menopausal women chronically ingesting high quantities of soy isoflavones. J Pharm Biomed Anal, 41;3: 957-965. McCarty, M. F. 2006. Isoflavones made simple - genistein's agonist activity for the beta-type estrogen receptor mediates their health benefits. Med Hypotheses, 66;6: 1093-1114. McMichael-Phillips, D. F., Harding, C., Morton, M., Roberts, S. A., Howell, A., Potten, C. S., & Bundred, N. J. 1998. Effects of soy-protein supplementation on epithelial proliferation in the histologically normal human breast. Am J Clin Nutr, 68;6 Suppl: 1431S-1435S. Messina, M., & Barnes, S. 1991. The role of soy products in reducing risk of cancer. J Natl Cancer Inst, 83;8: 541-546. Messina, M., McCaskill-Stevens, W., & Lampe, J. W. 2006. Addressing the soy and breast cancer relationship: review, commentary, and workshop proceedings. J Natl Cancer Inst, 98;18: 1275-1284. Messina, M. J., & Wood, C. E. 2008. Soy isoflavones, estrogen therapy, and breast cancer risk: analysis and commentary. Nutr J, 7: 17. Morali, G., Polatti, F., Metelitsa, E. N., Mascarucci, P., Magnani, P., & Marre, G. B. 2006. Open, noncontrolled clinical studies to assess the efficacy and safety of a medical device in form of gel topically and intravaginally used in postmenopausal women with genital atrophy. Arzneimittelforschung, 56;3: 230-238. Murkies, A. 1998. Phytoestrogens--what is the current knowledge? Aust Fam Physician, 27 Suppl 1: S47-51. Murkies, A. L., Lombard, C., Strauss, B. J., Wilcox, G., Burger, H. G., & Morton, M. S. 1995. Dietary flour supplementation decreases post-menopausal hot flushes: effect of soy and wheat. Maturitas, 21;3: 189-195. Murphy, P. A., Song, T., Buseman, G., Barua, K., Beecher, G. R., Trainer, D., & Holden, J. 1999. Isoflavones in retail and institutional soy foods. J Agric Food Chem, 47;7: 2697-2704. Muthyala, R. S., Ju, Y. H., Sheng, S., Williams, L. D., Doerge, D. R., Katzenellenbogen, B. S., Helferich, W. G., & Katzenellenbogen, J. A. 2004. Equol, a natural estrogenic metabolite from soy isoflavones: convenient preparation and resolution of R- and S-equols and their differing binding and biological activity through estrogen receptors alpha and beta. Bioorg Med Chem, 12;6: 1559-1567. Nedrow, A., Miller, J., Walker, M., Nygren, P., Huffman, L. H., & Nelson, H. D. 2006. Complementary and alternative therapies for the management of menopause-related symptoms: a systematic evidence review. Arch Intern Med, 166;14: 1453-1465.

Nestel, P. J., Pomeroy, S., Kay, S., Komesaroff, P., Behrsing, J., Cameron, J. D., & West, L. 1999. Isoflavones from red clover improve systemic arterial compliance but not plasma lipids in menopausal women. J Clin Endocrinol Metab, 84;3: 895-898. Occhiuto, F., Pasquale, R. D., Guglielmo, G., Palumbo, D. R., Zangla, G., Samperi, S., Renzo, A., & Circosta, C. 2007. Effects of phytoestrogenic isoflavones from red clover ;Trifolium pratense L. on experimental osteoporosis. Phytother Res, 21;2: 130-134. Ohta, T., Nakatsugi, S., Watanabe, K., Kawamori, T., Ishikawa, F., Morotomi, M., Sugie, S., Toda, T., Sugimura, T., & Wakabayashi, K. 2000. Inhibitory effects of Bifidobacterium-fermented soy milk on 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine-induced rat mammary carcinogenesis, with a partial contribution of its component isoflavones. Carcinogenesis, 21;5: 937-941. Overk, C. R., Guo, J., Chadwick, L. R., Lantvit, D. D., Minassi, A., Appendino, G., Chen, S. N., Lankin, D. C., Farnsworth, N. R., Pauli, G. F., van Breemen, R. B., & Bolton, J. L. 2008. In vivo estrogenic comparisons of Trifolium pratense ;red clover Humulus lupulus ;hops, and the pure compounds isoxanthohumol and 8-prenylnaringenin. Chem Biol Interact. Overk, C. R., Yao, P., Chadwick, L. R., Nikolic, D., Sun, Y., Cuendet, M. A., Deng, Y., Hedayat, A. S., Pauli, G. F., Farnsworth, N. R., van Breemen, R. B., & Bolton, J. L. 2005. Comparison of the in vitro estrogenic activities of compounds from hops ;Humulus lupulus and red clover ;Trifolium pratense. J Agric Food Chem, 53;16: 6246-6253. Palomares, M., Hopper, L., Goldstein, L., Lehman, C., Storer, B., & Gralow, J. 2004. Effect of soy isoflavones on breast proliferation in postmenopausal breast cancer survivors. Breast Cancer Res Treatment, 88: 4002. Pavese, J. M., Farmer, R. L., & Bergan, R. C. 2010. Inhibition of cancer cell invasion and metastasis by genistein. Cancer Metastasis Rev, 29;3: 465-482. Pearce, V., Nawaz, Z., Xiao, W., Wiedenfeld, D., Boyle, N., & Smith, D. 2003. 4-ethoxymethylphenol: a novel phytoestrogen that acts as an agonist for human estrogen receptors. J Steroid Biochem Mol Biol, 84;4: 431-439. Penotti, M., Fabio, E., Modena, A. B., Rinaldi, M., Omodei, U., & Vigano, P. 2003. Effect of soyderived isoflavones on hot flushes, endometrial thickness, and the pulsatility index of the uterine and cerebral arteries. Fertil Steril, 79;5: 1112-1117. Petrakis, N. L., Barnes, S., King, E. B., Lowenstein, J., Wiencke, J., Lee, M. M., Miike, R., Kirk, M., & Coward, L. 1996. Stimulatory influence of soy protein isolate on breast secretion in pre- and postmenopausal women. Cancer Epidemiol Biomarkers Prev, 5;10: 785-794. Pike, A. C., Brzozowski, A. M., Hubbard, R. E., Bonn, T., Thorsell, A. G., Engstrom, O., Ljunggren, J., Gustafsson, J. A., & Carlquist, M. 1999. Structure of the ligand-binding domain of oestrogen receptor beta in the presence of a partial agonist and a full antagonist. EMBO J, 18;17: 46084618. Power, K. A., & Thompson, L. U. 2003. Ligand-induced regulation of ERalpha and ERbeta is indicative of human breast cancer cell proliferation. Breast Cancer Res Treat, 81;3: 209-221. Powles, T. J., Howell, A., Evans, D. G., McCloskey, E. V., Ashley, S., Greenhalgh, R., Affen, J., Flook, L. A., & Tidy, A. 2008. Red clover isoflavones are safe and well tolerated in women with a family history of breast cancer. Menopause Int, 14;1: 6-12. Rachon, D., Vortherms, T., Seidlova-Wuttke, D., Menche, A., & Wuttke, W. 2007. Uterotropic effects of dietary equol administration in ovariectomized Sprague-Dawley rats. Climacteric, 10;5: 416-426. Rad, M., Humpel, M., Schaefer, O., Schoemaker, R. C., Schleuning, W. D., Cohen, A. F., & Burggraaf, J. 2006. Pharmacokinetics and systemic endocrine effects of the phyto-oestrogen 8-prenylnaringenin after single oral doses to postmenopausal women. Br J Clin Pharmacol, 62;3: 288-296. Rowland, I., Faughnan, M., Hoey, L., Wahala, K., Williamson, G., & Cassidy, A. 2003. Bioavailability of phyto-oestrogens. Br J Nutr, 89 Suppl 1: S45-58. Rowland, I. R., Wiseman, H., Sanders, T. A., Adlercreutz, H., & Bowey, E. A. 2000. Interindividual variation in metabolism of soy isoflavones and lignans: influence of habitual diet on equol production by the gut microflora. Nutr Cancer, 36;1: 27-32. Sammartino, A., Di Carlo, C., Mandato, V. D., Bifulco, G., Di Stefano, M., & Nappi, C. 2003. Effects of genistein on the endometrium: ultrasonographic evaluation. Gynecol Endocrinol, 17;1: 45-49. Santell, R. C., Chang, Y. C., Nair, M. G., & Helferich, W. G. 1997. Dietary genistein exerts estrogenic effects upon the uterus, mammary gland and the hypothalamic/pituitary axis in rats. J Nutr, 127;2: 263-269.

Sartippour, M. R., Rao, J. Y., Apple, S., Wu, D., Henning, S., Wang, H., Elashoff, R., Rubio, R., Heber, D., & Brooks, M. N. 2004. A pilot clinical study of short-term isoflavone supplements in breast cancer patients. Nutr Cancer, 49;1: 59-65. Sathyamoorthy, N., Wang, T. T., & Phang, J. M. 1994. Stimulation of pS2 expression by diet-derived compounds. Cancer Res, 54;4: 957-961. Scambia, G., Mango, D., Signorile, P. G., Anselmi Angeli, R. A., Palena, C., Gallo, D., Bombardelli, E., Morazzoni, P., Riva, A., & Mancuso, S. 2000. Clinical effects of a standardized soy extract in postmenopausal women: a pilot study. Menopause, 7;2: 105-111. Schmitt, E., Dekant, W., & Stopper, H. 2001. Assaying the estrogenicity of phytoestrogens in cells of different estrogen sensitive tissues. Toxicol In Vitro, 15;4-5: 433-439. Secreto, G., Chiechi, L. M., Amadori, A., Miceli, R., Venturelli, E., Valerio, T., & Marubini, E. 2004. Soy isoflavones and melatonin for the relief of climacteric symptoms: a multicenter, double-blind, randomized study. Maturitas, 47;1: 11-20. Setchell, K. D., Brown, N. M., & Lydeking-Olsen, E. 2002. The clinical importance of the metabolite equol-a clue to the effectiveness of soy and its isoflavones. J Nutr, 132;12: 3577-3584. Setchell, K. D., & Cassidy, A. 1999. Dietary isoflavones: biological effects and relevance to human health. J Nutr, 129;3: 758S-767S. Shah, S. H., & Alexander, K. P. 2003. Hormone Replacement Therapy for Primary and Secondary Prevention of Heart Disease. Curr Treat Options Cardiovasc Med, 5;1: 25-33. Simbalista, R. L., Sauerbronn, A. V., Aldrighi, J. M., & Areas, J. A. 2010. Consumption of a flaxseedrich food is not more effective than a placebo in alleviating the climacteric symptoms of postmenopausal women. J Nutr, 140;2: 293-297. Somekawa, Y., Chiguchi, M., Ishibashi, T., & Aso, T. 2001. Soy intake related to menopausal symptoms, serum lipids, and bone mineral density in postmenopausal Japanese women. Obstet Gynecol, 97;1: 109-115. St Germain, A., Peterson, C. T., Robinson, J. G., & Alekel, D. L. 2001. Isoflavone-rich or isoflavonepoor soy protein does not reduce menopausal symptoms during 24 weeks of treatment. Menopause, 8;1: 17-26. Steinberg, F. M., Murray, M. J., Lewis, R. D., Cramer, M. A., Amato, P., Young, R. L., Barnes, S., Konzelmann, K. L., Fischer, J. G., Ellis, K. J., Shypailo, R. J., Fraley, J. K., Smith, E. O., & Wong, W. W. 2010. Clinical outcomes of a 2-y soy isoflavone supplementation in menopausal women. Am J Clin Nutr. Sun, J. 2003. Morning/evening menopausal formula relieves menopausal symptoms: a pilot study. J Altern Complement Med, 9;3: 403-409. Takashima, N., Miyanaga, N., Komiya, K., More, M., & Akaza, H. 2004. Blood isoflavone levels during intake of a controlled hospital diet. J Nutr Sci Vitaminol ;Tokyo, 50;4: 246-252. Taku, K., Umegaki, K., Sato, Y., Taki, Y., Endoh, K., & Watanabe, S. 2007. Soy isoflavones lower serum total and LDL cholesterol in humans: a meta-analysis of 11 randomized controlled trials. Am J Clin Nutr, 85;4: 1148-1156. Tice, J. A., Ettinger, B., Ensrud, K., Wallace, R., Blackwell, T., & Cummings, S. R. 2003. Phytoestrogen supplements for the treatment of hot flashes: the Isoflavone Clover Extract ;ICE Study: a randomized controlled trial. JAMA, 290;2: 207-214. Unfer, V., Casini, M. L., Costabile, L., Mignosa, M., Gerli, S., & Di Renzo, G. C. 2004. Endometrial effects of long-term treatment with phytoestrogens: a randomized, double-blind, placebocontrolled study. Fertil Steril, 82;1: 145-148, quiz 265. Upmalis, D. H., Lobo, R., Bradley, L., Warren, M., Cone, F. L., & Lamia, C. A. 2000. Vasomotor symptom relief by soy isoflavone extract tablets in postmenopausal women: a multicenter, double-blind, randomized, placebo-controlled study. Menopause, 7;4: 236-242. USDA. 1998. USDA-Iowa State University Database on the Isoflavone Content of Foods. www.nal.usda.gov/fnic/foodcomp/Data/isofl/isoflav.html. Van de Weijer, P. H. M., & Barensten, R. 2002. Isoflavones from red clover ;Promensil significantly reduce menopausal hot flush symptoms compared with placebo. Maturitas, 42: 187-193. Van Patten, C. L., Olivotto, I. A., Chambers, G. K., Gelmon, K. A., Hislop, T. G., Templeton, E., Wattie, A., & Prior, J. C. 2002. Effect of soy phytoestrogens on hot flashes in postmenopausal women with breast cancer: a randomized, controlled clinical trial. J Clin Oncol, 20;6: 14491455. Washburn, S., Burke, G. L., Morgan, T., & Anthony, M. 1999. Effect of soy protein supplementation on serum lipoproteins, blood pressure, and menopausal symptoms in perimenopausal women. Menopause, 6;1: 7-13.

Weaver, C. M., Martin, B. R., Jackson, G. S., McCabe, G. P., Nolan, J. R., McCabe, L. D., Barnes, S., Reinwald, S., Boris, M. E., & Peacock, M. 2009. Antiresorptive effects of phytoestrogen supplements compared with estradiol or risedronate in postmenopausal women using ;41Ca methodology. J Clin Endocrinol Metab, 94;10: 3798-3805. Williamson-Hughes, P. S., Flickinger, B. D., Messina, M. J., & Empie, M. W. 2006. Isoflavone supplements containing predominantly genistein reduce hot flash symptoms: a critical review of published studies. Menopause, 13;5: 831-839. Woo, J., Lau, E., Ho, S. C., Cheng, F., Chan, C., Chan, A. S., Haines, C. J., Chan, T. Y., Li, M., & Sham, A. 2003. Comparison of Pueraria lobata with hormone replacement therapy in treating the adverse health consequences of menopause. Menopause, 10;4: 352-361. Wu, A. H., Wan, P., Hankin, J., Tseng, C. C., Yu, M. C., & Pike, M. C. 2002. Adolescent and adult soy intake and risk of breast cancer in Asian-Americans. Carcinogenesis, 23;9: 1491-1496. Wu, A. H., Yu, M. C., Tseng, C. C., & Pike, M. C. 2008. Epidemiology of soy exposures and breast cancer risk. Br J Cancer, 98;1: 9-14. Wu, A. H., Ziegler, R. G., Horn-Ross, P. L., Nomura, A. M., West, D. W., Kolonel, L. N., Rosenthal, J. F., Hoover, R. N., & Pike, M. C. 1996. Tofu and risk of breast cancer in Asian-Americans. Cancer Epidemiol Biomarkers Prev, 5;11: 901-906. Wu, J., Oka, J., Ezaki, J., Ohtomo, T., Ueno, T., Uchiyama, S., Toda, T., Uehara, M., & Ishimi, Y. 2007. Possible role of equol status in the effects of isoflavone on bone and fat mass in postmenopausal Japanese women: a double-blind, randomized, controlled trial. Menopause, 14;5: 866-874. Yamamoto, S., Sobue, T., Kobayashi, M., Sasaki, S., & Tsugane, S. 2003. Soy, isoflavones, and breast cancer risk in Japan. J Natl Cancer Inst, 95;12: 906-913. Zava, D. T., & Duwe, G. 1997. Estrogenic and antiproliferative properties of genistein and other flavonoids in human breast cancer cells in vitro. Nutr Cancer, 27;1: 31-40. Zhan, S., & Ho, S. C. 2005. Meta-analysis of the effects of soy protein containing isoflavones on the lipid profile. Am J Clin Nutr, 81;2: 397-408.