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Code No: R5312304 1

III B.Tech I Semester(R05) Supplementary Examinations, May 2009


GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. Explain the following with examples:

(a) Activators
(b) Repressors
(c) Promoter
(d) Operator
(e) Cis acting
(f) Trans acting
(g) polycistronic
(h) monocistronic. [2 × 8]

2. What are the other means by which gene rearrangements can take place apart from homologous
recombination? Explain in detail. [16]

3. What is the influence of transposon insertion on gene activity? Explain in detail. Also comment
on the association of disease with retroelement insertions. [16]

4. Write short notes on:

(a) expression vectors


(b) shuttle vectors
(c) phage vectors
(d) cosmid vectors. [4 × 4]

5. Explain the recent developments in DNA sequencing. [16]

6. What is a template? Discuss its role in DNA replication with a note on its quality in PCR. [16]

7. Discuss the concept of gene-chip and micro-arrays in detail. [16]

8. What are the currently available technologies for generation of transgenic animals?
[16]

?????
Code No: R5312304 2
III B.Tech I Semester(R05) Supplementary Examinations, May 2009
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. How do you define an operon? Explain the Control of gene expression in bacteria by citing the
example of any operon. [16]

2. Explain the following:

(a) Essential features of transcriptional activators

(b) Structural features of Transcription factor IIIA. [8 × 2]

3. Explain the basic mechanisms underlying transposition and excision. Add a note on applica-
tions of transposons. [16]

4. How would one go about if one wants to obtain a clone of a protein that is expressed in
eukaryotes say Interleukin? [16]

5. Discuss the strategies available for synthesis of RNA probes. [16]

6. Explain the different applications of PCR. [16]

7. What is meant by ribotyping? What is it’s major application? Explain. [16]

8. Discuss how one can clone an important protein like the factor VIII. [16]

?????
Code No: R5312304 3
III B.Tech I Semester(R05) Supplementary Examinations, May 2009
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. What is the need for employing different sigma factors as seen in B. subtilis? Explain the
regulation of sporulation in this system. [16]

2. What are the conditions under which gene amplification is likely to take place? Explain with
a suitable example. [16]

3. Write short notes on:

(a) replicative transposons


(b) excisive transposons. [8 × 2]

4. Write short notes on:

(a) expression vectors


(b) shuttle vectors
(c) phage vectors
(d) cosmid vectors. [4 × 4]

5. Write notes on:

(a) Southern blotting


(b) Northern Blotting
(c) Western Blotting
(d) Dot and slot blots. [4 × 4]

6. How does one identify a PCR product? In a particular PCR amplification experiment, the
product obtained was shorter than the expected length. Give your comments on the possible
reasons. [16]

7. How are RFLPs and VNTRs used in genetic fingerprinting? Explain. [16]

8. What are the currently available technologies for generation of transgenic animals?
[16]

?????
Code No: R5312304 4
III B.Tech I Semester(R05) Supplementary Examinations, May 2009
GENETIC ENGINEERING
(Bio-Technology)
Time: 3 hours Max Marks: 80
Answer any FIVE Questions
All Questions carry equal marks
?????

1. In which operon do you find the leader sequence? How does the translation of leader sequence
help in transcription control in certain operons? [16]

2. Explain the following:

(a) Essential features of transcriptional activators

(b) Structural features of Transcription factor IIIA. [8 × 2]

3. What is the influence of transposon insertion on gene activity? Explain in detail. Also comment
on the association of disease with retroelement insertions. [16]

4. Write short notes on:

(a) expression vectors

(b) shuttle vectors

(c) phage vectors

(d) cosmid vectors. [4 × 4]

5. Explain the dideoxy method in detail. [16]

6. Write a detailed account of the different markers used in disease diagnosis and fingerprinting.
[16]

7. What is the alternative to DNA arrays? Compare the technology and sensitivity of DNA arrays
and oligo chips. [16]

8. Discuss how one can clone an important protein like the factor VIII. [16]

?????

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