d ea R

ASRS 300 CSRS 300 QUICKSTART

A. Hydrating the Suppressor 1. 2.

is Th t! rs Fi

Hydrating the suppressor ensures that the ion exchange membranes are in a swollen form for proper operation. A 20 minute static step is recommended during first time installation to ensure complete hydration.

Using a disposable plastic syringe and the 10-32 Luer adapter (P/N 046888), push approximately 3 mL (SRS 4-mm) or 0.75 mL (SRS 2-mm) of degassed DI water through the ELUENT OUT port. Using a disposable syringe and the 1/4-28 Luer adapter (P/N 024305), push 5 mL (SRS 4-mm) or 2 mL (SRS 2-mm) of degassed DI water through the REGEN IN port, according to Figure 1. Step 1 can be accomplished by installing the suppressor in the system in the recycle mode, bypassing the guard and analytical columns, and pumping 5 mL (SRS 4-mm) or 2 mL (SRS 2-mm) of deionized water through the suppressor.

NOTE

1 CC

1.0 cc syringe Bolt and Ferrule

REGEN OUT ELUENT IN

Luer adapter Union DI H2O DI H2O

ANION SELF-REGENERATING
SUPPRESSOR ASRSULTRA 4-mm
P / N 0 6 156 1

DIONEX

ELUENTOUT

REGEN IN

P/N 016388 024305 046888 042627 043275 043276

Description 1 cc plastic syringe 1/4-28 Luer adapter 10-32 Luer adapter 10-32 Union 10-32 Bolt 10-32 Double Cone Ferrule

Figure 1 Hydrating the Suppressor 3. Allow the suppressor to sit for approximately 20 minutes to fully hydrate the suppressor screens and membranes. Do not install a luer adapter directly into a SRS 300 port. Connect a luer adapter to a union and use two 10-32 bolt and ferrules with a few centimeters of tubing to connect to the SRS ports.

WARNING
B.

Back Pressure Coil Pressure Test 1. 2. Disconnect the eluent line from the injection valve to the column at the column inlet. Connect the eluent line from the injection valve directly to the detector cell inlet with the recommended number of back pressure coils attached for your application (see the table below). Turn the pump on at your application flow rate. After 2 to 3 minutes of equilibration record pressure P1. Disconnect the back pressure coils and with the pump on measure the system pressure P2. 4-mm Chromatography 1.0 mL/min. = 2 black backpressure coils (P/N 045877) 2.0 mL/min. = 1 black backpressure coil (P/N 045877) 2-mm Chromatography____________ 0.25 mL/min. = 2 red backpressure coils (P/N 045878) 0.50 mL/min. = 1 red backpressure coil (P/N 045878)
Waste

Injection Valve

Cell

3.

Coil #1

Coil #2

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4.

The correct operating pressure range for the backpressure coil being tested is SRS 300 P1 - P2 = 3040 psi

Injection Valve

If the pressure is greater than 40 psi, then trim the back pressure coil and repeat step 2 and 3 to achieve 30-40 psi in step 4. If it is less than 30 psi, then add more tubing to achieve 30 - 40 psi. C. Quick Back Pressure Check Refer to section 3.2.3. in SRS 300 Product Manual P/N 031956
W as te

Cell

D. Column Purging 1. 2. If the column is new, purge column to waste with eluent for at least 10 minutes before connecting the column to the suppressor flow path. Connect the suppressor to your system in the recycle mode according to Figure 2. Recycle mode is recommended for all routine applications where solvent is not used in the eluent. Use the external water mode for all applications where solvent (< 40% v/v) is used in the eluent, analysis of borated waters or analysis of samples with complex matrices. Refer to the suppressor manual for more details.
Recommended tubing for various column formats 2-mm i.d., 10-32, 0.005" i.d. PEEK Liquid Line 3-mm i.d., 10-32, 0.005" i.d. PEEK Liquid Line 4-mm i.d., 10-32, 0.010" i.d. PEEK Liquid Line 5-mm i.d., 10-32, 0.010" i.d. PEEK Liquid Line
Injection Valve

NOTE

1/428 Tubing i.d.

1/4-28 1/4-28

Guard Column

W ast e

Gas Separator Wast e Tub e

Analytical Column
Backpressure Coils 1/428 Tubing i.d.
1/4-28 10-32 10-32 10-32 10-32 10-32 or 1/4-28

REGEN OUT 1/4-28 ELUENT I N 10-32

DIONEX
SELF-REGENE RATIN G SUPPRESSOR SRS LTRA U II
P/N 0xxxxx

ELUENT OU T 10-32 REGEN IN 1/4-28 10-32 or 1/4-28

Cell
10-32 or 1/4-28

Signal to Conductivity Detector

Figure 2 Connecting the Suppressor to the System in Recycle Mode (Optional) E. Connecting Suppressor and Power Settings Connect the suppressor to the power supply and set the power setting to the recommended current as defined for your eluent conditions. See suppressor manual for more details on power settings. The Recommended Maximum Current Setting for the SRS 300 2-mm is 100 mA. As a general operation precaution, never apply current to the SRS 300 without eluent or water regenerant flowing through the eluent and regenerant channel of the SRS 300.

CAUTION

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August 2007