2’Fluoro Phosphoramidites

for Proligo® Reagents

Product Information

In order to extend the existing amidite product line 2’Fluoro U and C,

SAFC Supply Solutions® adds two new monomers to its Proligo® Reagents
portfolio: A and G.

Figure A. 2’Fluoro-dA(bz) Amidite Figure B. 2’Fluoro-dG(ib) Amidite

O OMe
OMe O
HN
N
N NH O
N
N N N
N N MeO O
MeO O O H
O

O F O F
P O P O
N N
CN CN

Key Features of 2’Fluoro Amidites

• 2’Fluoro amidites are used to synthesize • 2’Fluoro amidites can be employed

oligonucleotides that are more thermally together with DNA and RNA amidites
stable and provide increased nuclease
• Compatible with deprotection
resistance
schemes based on methylamine
• All four bases are available: AMA (conc. aqueous ammonia/40%
- DMT-2’Fluoro-dA(bz) Phosphoramidite aqueous methylamine 1/1, v/v)

- DMT-2’Fluoro-dC(ac) Phosphoramidite • Lot consistent purity and performance

- DMT-2’Fluoro-dG(ib) Phosphoramidite • Manufactured under a certified ISO

9000:2000 quality system
- DMT-2’Fluoro-dU Phosphoramidite

Ordering Information Analytical specifications of 2’Fluoro Amidites

Description Unit Cat. No. Test 2’F-dA(bz) 2’F-dC(ac) 2’F-dG(ib) 2’F-dU

Compatible with ExpediteTM and Polygen® Instruments Reverse
Phase ≥ 98.0% ≥ 98.0% ≥ 98.0% ≥ 98.0%
DMT-2’Fluoro-dA(bz) Amidite 1 x 0.25g A211281-01
HPLC
DMT-2’Fluoro-dC(ac) Amidite 1 x 0.25g C213281-01
P-NMR
31
≥ 98% ≥ 99% ≥ 98% ≥ 99%
DMT-2’Fluoro-dG(ib) Amidite 1 x 0.25g G211281-01
DMT-2’Fluoro-dU Amidite 1 x 0.25g U211281-01
Compatible with ABI® Instruments
DMT-2’Fluoro-dA(bz) Amidite 1 x 0.25g A211231-01
DMT-2’Fluoro-dC(ac) Amidite 1 x 0.25g C213231-01
DMT-2’Fluoro-dG(ib) Amidite 1 x 0.25g G211231-01
DMT-2’Fluoro-dU Amidite 1 x 0.25g U211231-01 Sigma-Aldrich Biochemie GmbH
Georg-Heyken-Strasse 14
Other quantities and custom packaging are 21147 Hamburg, Germany
available upon request. +49 40 797 02 250 (Europe)
+1 877 776 5446 (US Toll Free)
Global Email: safcproligoreagents@sial.com

SAFC®, SAFC Supply Solutions®, Sigma-Aldrich® and SAFC Proligo® Reagents

are registered trademarks of Sigma-Aldrich Biotechnology L.P. and
Sigma-Aldrich Co. ABI® is a registered trademark of Applera Corporation or its
subsidiaries. Expedite™ is a trademark of Applera Corporation or its
subsidiaries. PolyGen® is a registered trademark of PolyGen GmbH.
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