TABLE

OF CONTENTS
Title BriefDescriptionsofModules Level1000ModuleWriteups Level2000ModuleWriteups Level3000ModuleWriteups Level4000ModuleWriteups InformationonLifeSciencesTeachingLabs MinimalLaboratorySafetyRequirements ListofModuleCoordinators LifeSciencesAdvisorsforLevels1,2,3,4 ListofSupportingStaff

Page 1 15 29 38 64 93 97 98 105 106

 NUS LIFE SCIENCES PROGRAMME

The NUS Life Sciences Programme is designed to provide NUS undergraduates with the fundamentals in biological and biomedical sciences. With a solid basic foundation acquired during the first two years of studies, selection of relevant advanced level courses would lead to areas of specialisation in Biomedical Science (BMS), Environmental Biology (EVB), or Molecular and Cell Biology (MCB) for students graduating with B.Sc. (Hons) Degree. Graduates of this programme would be well prepared to take on challenges in the knowledgebased economy and to contribute and support Singapores initiatives in Life Sciences. The Departments of Biological Sciences, Chemistry, and Applied Statistics and Probability in the Faculty of Science, and the Departments of Anatomy, Biochemistry, Epidemiology and Public Health, Microbiology, Pharmacology and Physiology in the Yong Loo Lin School of Medicine teach the UndergraduateLifeSciencesCurriculum. The curriculum is broadbased, integrated, streamlined and at the same time flexible. Core skills in the life sciences will be developed through emphasis on fundamental concepts and principles, laboratory competence and research techniques, as well as their applications in modern Life Sciences disciplines. The courseworkincorporatesallthemodernLifeSciencestopicsandbenchworkskillsaretaughtindedicated, stateoftheartteachinglaboratories.

*Life Sciences Website: http://www.lifesciences.nus.edu.sg

B.Sc. /B.Sc. (Hons.) in Life Sciences (For Matriculation Cohort AY 2011/2012) Along with the University and Faculty Requirements and Unrestricted Electives, to be awarded a B.Sc. (Honours) or B.Sc. with major in Life Sciences, candidates must satisfy the following: Major Requirements (For Matriculation Cohort AY 2011/2012) Level 1000 Pass LSM1101, LSM1102, LSM1104, CM1401* and ST1232*. LSM1103, Cumulative Major MCs 24

Level 2000

Pass LSM2101, LSM2102, LSM2103, LSM2201A or LSM2202A or LSM2203, and LSM2241 or LSM2251.

44

Level 3000

Pass 5 Level 3000 Life Sciences modules from any area of focus or Life Sciences Related modules, out of which at least 3 have to be from one chosen area of focus (BMS/MCB/EVB).

64

Level 4000
[For B.Sc. (Hons.)]

Pass the Honours Project LSM4199**, and 5 Level 4000 Life Sciences modules from any area of focus or Life Sciences Related modules, out of which at least 3 have to be from chosen specialisation (BMS/MCB/EVB).

100

University Requirements Faculty Requirements** Major Requirements Unrestricted Elective Modules Total

B.Sc. 20 MCs 4 MCs 64 MCs 32 MCs 120 MCs

B.Sc. (Hons.) 20 MCs 4-8 MCs 100 MCs 32-36 MCs 160 MCs

* CM1401 and ST1232 satisfy 8 MCs of the Faculty Requirements as well as the Major Requirements. Please DO NOT bid and read ST1131. ** To qualify for Honours year and LSM4199, students must fulfill the major requirements of Life Sciences at B.Sc. level, and obtained a minimum overall CAP of 3.50 on completion of 100 MCs (Modular Credits) or more. Note: The number of MCs earned from Level 1000 modules for graduation requirements is capped at 60. Faculty Requirements for Life Sciences Major: Please refer to the following web page for details and subject groupings: http://www.nus.edu.sg/registrar/nusbulletin/FoS/UG/degreqs.html#3_3_1F B.Sc. Modules that can be used to fulfill Faculty Requirements (12 MCs): Module 1: CM1401 (4MC) Module 2: ST1232 (4MC) Module 3: 1 module from Physical Sciences OR Computing Sciences OR Multidisciplinary & Interdisciplinary Sciences subject group (4MC) B.Sc. (Hons.) Modules that can be used to fulfill Faculty Requirements (16 MCs): Module 1: CM1401 (4MC) Module 2: ST1232 (4MC) Module 3: 1 module from Physical Sciences OR Computing Sciences OR Multidisciplinary & Interdisciplinary Sciences subject group (4MC) Module 4: 1 module from any subject group except LSM-prefixed modules (4MC)
(Note: If FST3102 or FST3203 is read to fulfill major requirements, this module can be counted as fulfilling this Module 4 of Faculty Requirement; an additional 4MC of UE has to be read in this case.)

TobeawardedatB.Sc.(Hons.)levelaprimarymajorinLifeSciences(withspecialisationinBiomedicalScience,MolecularandCellBiologyor EnvironmentalBiology),candidatesmustsatisfythefollowing[For Matriculation Cohort AY 2011/2012]:

Level 1000 Pass all modules below LSM1101 LSM1102 LSM1103 Level 2000 Biochemistry of Biomolecules Molecular Genetics Biodiversity LSM1104 CM1401 ST1232 Pass 1 module below LSM2201A LSM2202A LSM2203 Level 3000 Experimental Biochemistry Experimental Molecular and Cell Biology Experimental Microbiology General Physiology Chemistry for Life Sciences Statistics for Life Sciences Pass 1 module below LSM2241 LSM2251 Introductory Bioinformatics Ecology and Environment

Pass all modules below LSM2101 LSM2102 LSM2103 Metabolism and Regulation Molecular Biology Cell Biology

Pass 5 Level 3000 Life Sciences modules from any area of focus or Life Sciences Related Modules, out of which at least 3 have to be from one chosen area of focus (BMS/MCB/EVB). Biomedical Science (BMS) LSM3211 LSM3212 LSM3213 LSM3214 LSM3221 LSM3223 LSM3224 LSM3225 Fundamental Pharmacology Human Physiology Cardiopulmonary System Molecular and Cellular Neurobiology Human Physiology Hormones and Health Human Pharmacology Immunology Molecular Basis of Human Diseases Molecular Microbiology in Human Diseases BN3301 BN3402 FST3102 FST3203 Molecular and Cell Biology (MCB) LSM3231 LSM3232 LSM3233 LSM3241 LSM3242 LSM3243 LSM3244 Protein Structure and Function Microbiology Developmental Biology Bioinformatics and Biocomputing Applied Microbiology Molecular Biophysics Molecular Biotechnology Environmental Biology (EVB) LSM3252 LSM3253 LSM3254 LSM3255 LSM3256 LSM3261 LSM3262 LSM3263 LSM3272 Evolution and Comparative Genomics Plant Physiology Ecology of Aquatic Environments Ecology of Terrestrial Environments Tropical Horticulture Life Form and Function Environmental Animal Physiology Field Studies in Neotropical Ecosystems Global Change Biology

Life Sciences Related Modules:

Introduction to Biomaterials Bio-Analytical Methods in Bioengineering Food Safety Assurance Vitamins & Minerals in Health & Diseases

LSM3288/LSM3289 Advanced UROPS in Life Sciences Note: 4MCs read for Level 3000 UROPS can satisfy 1 Level 3000 major module of any area of focus.

Level 4000

Pass LSM4199 Honours Project in Life Sciences Pass 5 Level 4000 Life Sciences modules from any area of focus or Life Sciences Related Modules, out of which at least 3 have to be from chosen specialisation (BMS/MCB/EVB). Biomedical Science (BMS) LSM4211 LSM4212 LSM4213 LSM4214 LSM4221 LSM4222 LSM4223 LSM4224 LSM4225 LSM4226 Toxicology Pharmacogenetics and Drug Responses System Neurobiology Cancer Pharmacology Drug Discovery and Clinical Trials Advanced Immunology Advances in Antimicrobial Strategies Free Radicals and Antioxidant Biology Genetic Medicine in the PostGenomic Era Infection and Immunity BN4301 BN4403 Molecular and Cell Biology (MCB) LSM4231 LSM4232 LSM4233 LSM4241 LSM4242 LSM4243 LSM4251 LSM4252 Structural Biology Advanced Cell Biology Chemical Biology Functional Genomics Protein Engineering Tumour Biology Plant Growth and Development Animal Reproduction Environmental Biology (EVB) LSM4253 LSM4254 LSM4261 LSM4262 LSM4263 LSM4264 LSM4265 Behavioural Biology Principles of Taxonomy and Systematics Marine Biology Tropical Conservation Biology Field Studies in Biodiversity Freshwater Biology Urban Ecology

Life Sciences Related Modules:

Principles of Tissue Engineering Cellular Bioengineering

TobeawardedatB.Sc.levelaprimarymajorinLifeSciences,candidatesmustsatisfythefollowing[For Matriculation Cohort AY 2011/2012]:

Level 1000 Pass all modules below LSM1101 LSM1102 LSM1103 Level 2000 Biochemistry of Biomolecules Molecular Genetics Biodiversity LSM1104 CM1401 ST1232 Pass 1 module below LSM2201A LSM2202A LSM2203 Level 3000 Experimental Biochemistry Experimental Molecular and Cell Biology Experimental Microbiology General Physiology Chemistry for Life Sciences Statistics for Life Sciences Pass 1 module below LSM2241 LSM2251 Introductory Bioinformatics Ecology and Environment

Pass all modules below LSM2101 LSM2102 LSM2103 Metabolism and Regulation Molecular Biology Cell Biology

Pass 5 Level 3000 Life Sciences modules from any area of focus or Life Sciences Related Modules, out of which at least 3 have to be from one chosen area of focus (BMS/MCB/EVB). [LSM42XX modules from the chosen area may be taken to replace up to 8 MCs of these five modules.] Biomedical Science (BMS) LSM3211 LSM3212 LSM3213 LSM3214 LSM3221 LSM3223 LSM3224 LSM3225 Fundamental Pharmacology Human Physiology Cardiopulmonary System Molecular and Cellular Neurobiology Human Physiology Hormones and Health Human Pharmacology Immunology Molecular Basis of Human Diseases Molecular Microbiology in Human Diseases BN3301 BN3402 FST3102 FST3203 Molecular and Cell Biology (MCB) LSM3231 LSM3232 LSM3233 LSM3241 LSM3242 LSM3243 LSM3244 Protein Structure and Function Microbiology Developmental Biology Bioinformatics and Biocomputing Applied Microbiology Molecular Biophysics Molecular Biotechnology Environmental Biology (EVB) LSM3252 LSM3253 LSM3254 LSM3255 LSM3256 LSM3261 LSM3262 LSM3263 LSM3272 Evolution and Comparative Genomics Plant Physiology Ecology of Aquatic Environments Ecology of Terrestrial Environments Tropical Horticulture Life Form and Function Environmental Animal Physiology Field Studies in Neotropical Ecosystems Global Change Biology

Life Sciences Related Modules:

Introduction to Biomaterials Bio-Analytical Methods in Bioengineering Food Safety Assurance Vitamins & Minerals in Health & Diseases

LSM3288/LSM3289 Advanced UROPS in Life Sciences Note: 4MCs read for Level 3000 UROPS can satisfy 1 Level 3000 major module of any area of focus.

GENERAL INFORMATION

Unrestrictedmodulesaremodulesstudentstaketotopupcreditstoreachatotalof120MC/160MC. OnlyextraGroupBGEMsandextraSSmodulescanbeusedtofulfillBreadthrequirements. PleaserefertoURL:http://www.science.nus.edu.sg/undergraduates/faq/index.html#UE StudentsmaychoosealltheirBreadthmodulesfromasinglefaculty. FormoreinformationonfacultyrequirementspleaserefertoURL: http://www.science.nus.edu.sg/undergraduates/faq/index.html#FR Studentsdonotneedtobidforspecialtermmodules.Theirapplicationswillbesubjecttocomputerballoting.More informationregardingspecialtermwillbereleasednearertodate.StudentswillbenotifiedviaNUSemailaccount. TheMCsearnedforstudentsminorrequirementsarepartofthe120/160MCrequiredforgraduation.LSmajorstudents canchoosetostudyminorsofferedbytheUniversityasanoptioniftheyqualifyforthem.

Exemptions from University Level Requirements and Unrestricted Elective Modules (Only applicable to polytechnic diploma holders) PolytechnicDiplomaholdersadmittedtotheFacultywillbeautomaticallygrantedadvancedplacementcreditsof20 MCs(notsubjecttoperformanceinplacementtests)asfollows: i.8MCsfromUniversityLevelRequirements(oneGroupBGEMandoneBreadthModule)* ii.12MCsfromUnrestrictedElectiveModules(UEM). *All20MCsofadvancedplacementcreditsaretaggedatLevel1000.However,studentsshouldnotethatonly the8MCsfromUniversityLevelRequirementswillbecountedagainstthe60MCslimitthatstudentsareallowedto readinfulfilmentofthe120/160MCsrequiredforgraduation.The12MCsfromUnrestrictedElectiveModuleswill notbecountedagainstthe60MCslimitthatstudentsareallowedtoread. Workload i. Minimum workload: 15 MCs per semester. Students are only allowed to read less than 15 MCs in their graduating semester.Recommendedworkload:20MCspersemester. ii.Existingstudentswishingtoreadmorethan26MCsmusthaveaCAPofatleast3.50. iii. Newlymatriculated students who wish to read more than 26 MCs must seek approval from the Science Deans OfficeviatheCentralisedOnlineRegistrationSystem(CORS).

Brief descriptions of modules offered in AY2011/2012 LSM1101 BIOCHEMISTRY OF BIOMOLECULES (Essential, 4MC) Prerequisite:GCEAlevelBiologyorH2BiologyorequivalentorLSM1301 Workload:26lecturehours+6tutorialhours+18laboratoryhours This module provides an overview of pH and buffers, and the physicalchemical basis of biological molecules: nucleic acids, proteins, carbohydrates and lipids; their structurefunction relationships. Enzymes, enzyme kinetics and applications of enzymes, heme biosynthesis and metabolism will also be covered. LSM1102 MOLECULAR GENETICS (Essential, 4MC) Prerequisite:GCEAlevelBiologyorH2BiologyorequivalentorLSM1301 Workload:26lecturehours+24practicalandtutorialhours Thecoursewillcovertopicson(i)thepatternsofinheritance,(ii)themolecularstructureandreplicationof genetic material, (iii) the molecular properties of genes, and (iv) genetic analysis of individuals and populations. This will include an indepth understanding of Mendelian patterns of inheritance and variations that could occur due to multiple alleles, lethal genes, chromosomal variations, linkage, gene interaction and other genetic phenomena. Emphasis is placed on the understanding of the underlying molecular and biochemical basis of inheritance. Quantitative and population genetics will also be discussed with the emphasis of understanding the processes and forces in nature that promote genetic change. LSM1103 BIODIVERSITY (Essential, 4MC) Prerequisite:GCEAlevelBiologyorH2BiologyorequivalentorLSM1301 Workload:26lecturehours+6tutorialhours+18laboratoryhours This course provides life science majors with a critical introduction to the diversity of life on earth. The means to identify and differentiate major groups of living is emphasised. Unique characteristics about the life history and ecology of these groups will be discussed through local and regional examples and field tripswillprovideanopportunitytoexplorethebiodiversityofselectedecosystemsaroundSingapore.This foundation in biodiversity integrates conservation issues such as the biodiversity crisis to provide an understandingoftheneedforadiverseandintricatebalanceofnature. LSM1104 GENERAL PHYSIOLOGY (Essential, 4MC) Prerequisite:GCEOLevelBiologyorLSM1301 Workload:26lecturehours+2CAhours+4tutorialhours+18laboratoryhours This module deals with General Physiology and its theme is Biological Transducers and Energy Transformation. This module will start with bioenergetics which stresses on the application of thermodynamics to physiological processes in both animals and plants. Six types of energy will be dealt with,concerning(1)thetransformationoflightenergytochemicalenergybyplants,(2)thetransformation of chemical energy to chemical potential energy of ions and water across biomembranes, (3) the transformationofchemicalpotentialenergytoelectricalenergybyplasmalemmawithspecialemphasison neurons, (4) the transformation of chemical energy to mechanical energy during animal locomotion, and (5) and the production and release of heat during energy transformation. In this module, the important concept of homeostasis, with special emphasis on the balance of heat gain and heat loss to maintain a constantbodytemperaturewillbecovered. LSM1202 HUMAN ANATOMY (Elective, 4MC) Prerequisite:GCE'O'levelBiologyorLSM1301 Workload:26lecturehours+22laboratoryhours This module provides a basic introduction to human structure and function, comprising gross anatomy integrated with microscopic anatomy. Histological organization of the primary tissues: epithelial,
1

connective, muscular and nervous tissues will also be covered. Clinical relevance of the anatomical structureswillbediscussed. *LSM1301 GENERAL BIOLOGY (Elective, 4MC) Preclusion: Must not have read GCE 'A' Level or H2 Biology, or equivalents. Cannot be read concurrently withLSM1101,LSM1102,LSM1103,LSM1104andLSM1202. Workload:24lecturehours+26tutorialhours(practicalsessions,assignments,andmuseumvisit) Thisisanintroductorymodulethatexploreswhatalivingthingis,thebasicsoflife,andthesciencebehind it. The course will introduce the chemistry of life and the unit of life. The question of how traits are inherited will be discussed and the field of biotechnology, including its applications and the ethical issues involved will be introduced. The diversity of life on earth will be explored, withdiscussionson how life on earth possibly came about and how biologists try to classify and make sense of the diversity. The course will also introduce the concept of life functions from cells to tissues and from organs to systems. The concept of how organisms maintain their internal constancy and organisation of major organ systems will be discussed. The focus will be to introduce the unifying concepts in biology and how they play a role in everydaylife. *LSM1302/GEK1527 GENES AND SOCIETY (Elective, 4MC) Preclusion:NotforstudentstakingLifeSciencesmajor/minor,FST,PharmacyandBioengineeringmajor. Workload: 26 lecture hours + 24 hours of Tutorials, Interactive Learning & Feedback for CAs (CDROM assistedLearning,WebbasedInteractiveLearning,Assignments,Quizzes,etc.) The aim of the module is to introduce students to the modern concepts in biology and to enable them to evaluate independently the potential benefits and risks of the biotechnological revolution and its implications for society. It focuses on how genes and our understanding of it have impacted, are and will be impacting our lives as individuals and our society at large. While the module covers broad themes of genetics and biotechnology including related ethical issues and implications, it also provides sufficient grounding of fundamental concepts to ensure students of diverse backgrounds can appreciate the significanceofpast,presentandfuturedevelopmentsofgeneticknowledgeonsocieties. *LSM1303 ANIMAL BEHAVIOR (Elective, 4MC) Preclusion: Not for students with GCE A level Biology or H2 Biology or have passed LSM1301 or Life Sciencesmajor/minororFoodSciences&TechnologyorPharmacymajorsorBioengineeringprogram. Workload:26lecturehours+4tutorialhours+6laboratoryhours+14groupprojectfieldtriphours Anunderstandingofananimalbehaviorprovidesanappreciationoftheuniquenessofaparticularspecies and other similar species. How an animal's behavior is best suited to a particular ecological situation is explored through fundamental and current theories including optimality, natural and sexual selection. A diversity of animal behaviors are explored such as learning, communication, territoriality and courtship. Research methods will also be discussed and explored through student projects. The knowledge gained through animal behavior can be translated into better animal care and conservation, and perhaps provide abetterinsightintoourownbehavior. *LSM1401 FUNDAMENTALS OF BIOCHEMISTRY (Elective, 4MC) Prerequisite: For Bioengineering, Chemical Engineering, Engineering Science Programme, Environmental Science and Engineering, Chemistry and Pharmacy students only. Pass in GCE 'A' Level or H2 Chemistry or equivalents. Preclusion:NotforLifeSciencesmajor/minor(exceptChemistrymajor)andstudentmustnothaveread LSM1101oritsequivalent. Workload:28lecturehours+10tutorialhours(assignments,classes,etc.)+3laboratorysessions This module aims to provide the student with a strong background in the fundamental aspects of the biochemistry of biomolecules including selected topics of cell biology, microbial systems, and molecular genetics with an emphasis on their application to chemical and pharmaceutical industries as well as
2

engineering practices (in particular bioengineering, chemical engineering, environmental engineering, and engineering science). Upon completing this module, the student is expected to have sufficient knowledge of fundamental life processes in order to appreciate and relate the importanceof biochemistry in industry as well as in everyday life. The student should also be well prepared to take other advanced modules as wellinwhichbiochemistryisaprerequisite. CM1401 CHEMISTRY FOR LIFE SCIENCES (Essential, 4MC) Prerequisite:GCEAlevelorH2ChemistryorequivalentorCM1417 Workload:39lecturehours+13tutorialhours+15laboratoryhours ThisisachemistrymodulecateredforLifeSciencestudentsanddealsprimarilywiththebasicprinciplesto understand the structure and reactivity of organic molecules, basic thermodynamics and kinetics, and some basic analytical techniques. Students should acquire fundamental knowledge in chemistry for applicationstobiologicalsystems. ST1232 STATISTICS FOR LIFE SCIENCES (Essential, 4MC) Prerequisite:GCEAOlevelorH1passinMathematicsoritsequivalent Workload:39lecturehours+11tutorialhours An introduction to the basic principles and methods of biostatistics designed specifically for life sciences students who wish to learn modern research methods for analyzing and extracting information from biological, biomedical and genomic data. Relevant examples will be used throughout the course to illustrate various techniques. Two computer packages will be used to enhance learning and to enable students to analyze real life data sets. Topics include tabular and graphical display of data, probability and probability distributions, confidence intervals, hypothesis testing, distribution free tests, categorical data analysis,andregressionanalysis. THE ABOVE MODULES WITH AN (*) ARE NOT FOR LIFE SCIENCES MAJORS LSM2101 METABOLISM AND REGULATION (Essential, 4MC) Prerequisite:LSM1101orLSM1401 Workload:39lecturehours+11tutorialhours Overview of the biosynthesis and catabolism of carbohydrates, proteins, lipids and nucleic acids in the contextofhumanhealthanddisease.Emphasisontheintegrationandregulationofmetabolicpathwaysin different tissues and organs. Principles of bioenergetics and mitochondrial energy metabolism, free radicals,mitochondrialDNAdamageinagingandneurodegenerativediseaseswillalsobecovered. LSM2102 MOLECULAR BIOLOGY (Essential, 4MC) Prerequisite:LSM1101(orLSM1401)andLSM1102 Workload:39lecturehours+6tutorialhours+5CAandDiscussion Thismoduledealswiththestructure,organizationandfunctionofgenesandgenomesinbothprokaryotes and eukaryotes (eg: DNA topology, hierarchy of packaging of DNA in chromosomes and relationship to gene activity and genome dynamics). The functional roles of DNA regulatory ciselements and transcription factors involved in gene expression will be examined extensively. The molecular events of transcription; posttranscriptional modifications and RNA processing; temporal and spatial gene expression,controlandregulation,signalsofgeneexpressionwillbedealtwithindetail.Thecauseand/or effectofdysfunctionofgeneexpressionanddiseaseswillbediscussed. LSM2103 CELL BIOLOGY (Essential, 4MC) Prerequisite:LSM1101(orLSM1401)andLSM1102 Workload:39lecturehours+11tutorialhours This course provides a comprehensive understanding of cellular structures, functions and interactions in unicellular and multicellular systems. Emphasis is on cellular functions. Topics include structure and
3

functions of organelles, the cytoskeleton and cell movements, current concepts of intercellular and intracellular signaling, intracellular protein trafficking and organelle biogenesis (including endocytosis, exocytosis, import of proteins into organelles, nuclear transport), molecular basis of cell proliferation and apoptosis. LSM2201A EXPERIMENTAL BIOCHEMISTRY (Elective, 4MC) Prerequisite:LSM1101orLSM1401 Workload:12lecturehours+8tutorialhours+30laboratoryhours Emphasis on principles and understanding of methods of protein purification and characterization. The module integrates factual knowledge in various chromatographic methods to laboratory practice. Analysis andpresentationofdatafromminiprojectandfromjournalarticles. LSM2202A EXPERIMENTAL MOLECULAR AND CELL BIOLOGY (Elective, 4MC) Prerequisite:LSM1101and/orLSM1102 Workload:12lecturehours+30laboratoryhours+8tutorialhours This module introduces students to the theory and practical applications of techniques relevant to molecular and cell biology. The module emphasizes problemsolving exercises in the application of commonly used recombinant DNA techniques, including RNA isolation and characterization, reverse transcription, polymerase chain reaction (PCR), construction of recombinant DNA molecules, gel electrophoresis of RNA and DNA, DNA sequencing and analysis, and realtime PCR. The objective for the students is to learn HOW to perform the experiments, and to understand WHY these experiments have to becarriedoutinacertainway. LSM2203 EXPERIMENTAL MICROBIOLOGY (Elective, 4MC) Prerequisite:LSM1101orLSM1102orLSM1103 Workload:13lecturehours+32laboratoryhours This module gives an overview of microbial diversity, the biological properties of microbes, methods and approaches in the study of microbiology. At the end of the module, students should have fundamental knowledge of microbiology, including tools in the study of cells and microbes and the awareness of biosafety,andstudentsshouldbeexcitedbythemicrobialworldandwishingtoknowmore. LSM2241 INTRODUCTORY BIOINFORMATICS (Elective, 4MC) Prerequisite:LSM1101orLSM1401 Workload:22lecturehours+40laboratoryhourswithPresentation(CA1)andMCQMidterm(CA2) Introduction to biological databases and bioinformatics software. Sequence comparison algorithms and tools. Biomolecular 3D structure and modelling. Students will be introduced to bioinformatics theory, tools, and techniques. Practical Bioinformatics cover database searching, sequence analysis via EMBOSS, BLAST and BLAST database building,Multiple Sequence Alignment with CLUSTALW and JalView, Molecular Phylogenetic Inference with MEGA, PyMol structural visualization and Homology Modelling and Structure Prediction with MODELLER and SWISSMODEL. Students will achieve highly valued skills as biological researchers with basic competence in computational and informatics techniques, with an option to learn moreadvanceskillsinLSM3241BioinformaticsandBiocomputing. LSM2251 ECOLOGY AND ENVIRONMENT (Elective, 4MC) Prerequisite:LSM1103 Workload:26lecturehours+6tutorialhours+18practicalhoursincludingoneSaturdayfieldtrip This module introduces students to the science of ecology and its role in understanding environmental processes. It covers both the major concepts and their realworld applications. Topics will include models in ecology, organisms in their environment, evolution and extinction, life history strategies, population biology, ecological interactions, community ecology, ecological energetics, nutrient cycling, landscape ecology.
4

 LSM3211 FUNDAMENTAL PHARMACOLOGY (Elective, 4MC) Prerequisite:LSM2101orLSM2103 Preclusion:GEK2501 Workload:26lecturehours+6tutorialhours+18laboratoryhours Thismoduleaimstoprovidebasicprinciplesofpharmacologicalsciencewithemphasisondrugactionfrom the molecular and cellular levels to tissue, organ and whole animal including humans using lectures and practicals. The lectures will start with the classical drug receptor theory followed by molecular pharmacology of drug receptors and their regulation including receptormediated signal transduction and membrane ion channel function. Autonomic pharmacology (sympathetic and parasympathetic) will be introduced. The module also focuses on the pharmacodynamics of autacoids, nonsteroidal anti inflammatory agents, corticosteroids and other immunosuppressants, antiasthma drugs, antithrombotic agentsandnitricoxide. LSM3212 HUMAN PHYSIOLOGY: CARDIOPULMONARY SYSTEM (Elective, 4MC) Prerequisite:LSM2101 Workload: 24 lecture hours + 6 tutorial hours + 10 practical hours + 10 hours assignments/Self directed study The heart and lungs are central to the maintenance of homeostasis in the human body by bringing essential materials to and removing wastes from the bodys cells. This module covers the basic physiology of the cardiovascular and pulmonary systems using exercise to illustrate the onset of homeostatic imbalancesandthebodysresponsestorestorehomeostasis.Studentswillbeabletoidentifythebenefits thatexerciseimpartstocardiorespiratoryfitnessandoverallhealth. LSM3213 MOLECULAR & CELLULAR NEUROBIOLOGY (Elective, 4MC) Prerequisite:LSM2102andLSM2103 Workload:26lecturehours+12tutorialhours+12practical/SDLhours This module aims firstly to introduce third year students to neurobiology and secondly to impart fundamental concepts and principles in neurophysiology, neuropharmacology, neurochemistry and cell biology of the nervous system. The fascination with how the brain works remains to this day and it is still very much a mystery. The first step on this quest begins with understanding the fundamental principles underlying the molecular and cellular functions of neuronal and glial cells, thebuilding blocks of thebrain. This module seeks (1) to inform; (2) to challenge on the basis that scientific enquiry is uncertain while knowledge is tentative and (3) to relate neurobiology to our lives. The topics include: (1) Neuronal signalling;(2)Mechanismsofneurotransmission;(3)Neuronalgrowth,regenerationanddegenerationand (4)Glialfunctionandneurogrowthfactors. LSM3214 HUMAN PHYSIOLOGY: HORMONES AND HEALTH (Elective, 4MC) Prerequisite:LSM2101andLSM3212.NocoreadingofLSM3212andLSM3214 Workload:26lecturehours+14tutorialhours+10hoursassignmentand3CAs This module covers several human physiological systems using hormonal control of homeostasis as a basis for understanding normal function and health. The student will be able to appreciate the interactions occurringamongsttheendocrine,digestive,renal,andreproductivesystems,andbeabletorelatethemto the body's biological rhythms (or clocks), growth, responses to stress, and reproductive processes. Major Topics Covered: endocrine system, central endocrine glands, peripheral endocrine glands, digestive system, digestive processes, energy balance, urinary system, fluid processing, fluid balance, reproductive system,malereproductivephysiologyandfemalereproductivephysiology.

LSM3221 HUMAN PHARMACOLOGY (Elective, 4MC) Prerequisite:LSM3211 Workload:26lecturehours+6tutorialhours+18practicalhours This module introduces the pharmacological treatment of human disease. We will look at actions of drugs on major organsystems in the human body. Examples of drugs used to treat diseases and disordersof the cardiovascular,endocrineandnervoussystemsandchemotherapeuticagentswillbediscussed. LSM3223 IMMUNOLOGY (Elective, 4MC) Prerequisite:LSM2103 Workload:24lecturehours+24laboratoryhours+2tutorialhours This course provides the central concepts of immunology and the foundation for understanding how immunity functions. The subjects of innate immunity and haematopoiesis introduce the origin and role of different cell types in immunity. The mechanics of how the body protects itself from disease are explored in relation to T and B cell biology, antibodyantigen interaction, major histocompatibility complex and antigen presentation. Other topics include hypersensitivity, immunodeficiencies, immune tolerance, autoimmunity,resistanceandimmunizationtoinfectiousdiseases. LSM3224 MOLECULAR BASIS OF HUMAN DISEASES (Elective, 4MC) Prerequisite:PassinLSM2101andLSM2103 Workload:26lecturehours+6tutorialhours+18laboratoryhours This module aims to provide students with indepth knowledge of the basic mechanisms of common human diseases, such as cancer, atherosclerosis, obesity, diabetes, muscle wasting conditions and to preparethemforfuturetranslationalresearch.Thismodulefocusesonthecurrentmolecularmechanisms underlying the pathogenesis of each disease. There will be extensive discussion on results from current cuttingedge research. Prospective students should have basic knowledge of molecular and cell biology, geneticsandgeneralhumanphysiologybeforeregisteringforthismodule. LSM3225 Molecular Microbiology in Human Diseases (Elective, 4MC) Prerequisite:LSM2101orLSM2102orLSM2103 Workload:24lecturehours+16laboratoryhours+2tutorialhours With the application of advanced technologies in molecular biology to the study of microorganisms, there are many implications on how we can identify and detect microbes, as well as treat and prevent diseases caused by both existing and newly emerged pathogens. In this course, the students will be taught the molecular principles of the physiological processes involved in the life cycle of different types of microbes and how these affect human health and disease. There are also practicals and specialized talks by guest lecturers to emphasis the application of molecular microbiology in laboratories that handle the diagnosis andsurveillanceofinfectiousdiseases. LSM3231 PROTEIN STRCUTURE AND FUNCTION (Elective, 4MC) Prerequisite:LSM2101 Workload:26lecturehours+6tutorialhours+18laboratoryhours The main objective is to provide a strong foundation in the study of protein structure and function. The following topics will be covered: Structures and structural complexity of proteins and methods used to determine their primary, secondary and tertiary structures; Biological functions of proteins in terms of their regulatory, structural, protective and transport roles; The catalytic action of enzymes, their mechanism of action and regulation; Various approaches used in studying the structurefunction relationshipofproteins.

LSM3232 MICROBIOLOGY (Elective, 4MC) Prerequisite:LSM2101orLSM2102orLSM2103 Workload:26lecturehours+21laboratoryhours+2tutorials+1CA Principles of microbiology, with emphasis on the properties, functions and classification of the major classes of microorganisms, especially bacteria, fungi and viruses. Understanding microbial activities and theirinfluenceonmicrobialdiseases,industrialapplications,ecology,foodandwaterquality. LSM3233 DEVELOPMENTAL BIOLOGY (Elective, 4MC) Prerequisite:LSM2103 Workload:26lecturehours+6tutorialhours+18laboratoryhours This course will showcase and examine embryogenesis, starting from fertilization to birth in the case of animaldevelopment;andtogermination,growthanddifferentiationinplants.Studentswillbeexposedto concepts, principles and mechanisms that underlie development in plants and animals. Different model organismswillbestudiedtodemonstratetherapidadvancesinthisfieldoflifesciences. LSM3241 BIOINFORMATICS & BIOCOMPUTING (Elective, 4MC) Prerequisite:LSM2102andeitherLSM2104orLSM2241 Workload:26lecturehours+30tutorialhours/laboratoryhours This module provides intermediate to advanced topics in bioinformatics. Topics include sequence analysis and applications, basic programming for bioinformatics applications, molecular modeling and drug design, protein modeling, data mining and machine learning in biomedical sciences. Besides handson practical classes, students are involved in a project in which skills taught in the course will be applied to solve a biological research problem. Students of this module achieve proficiency in computational skills which are highlysoughtafterintodayscompetitivebiologicalresearchlaboratories. LSM3242 APPLIED MICROBIOLOGY (Elective, 4MC) Prerequisite:LSM3232andeitherLSM2101orLSM2102orLSM2103.(LSM3232canbereadascorequisite) Workload:24lecturehours+8tutorialhours/industrialvisits+18laboratoryhours This module covers the underlying scientific principles and the wideranging industrial, environmental, pharmaceutical, and biomedical applications of microbiology. The objectives are (a) to gain an understanding of the role of microorganisms for biotechnology applications and (b) to review advances in genetics and molecular biology of industrial microorganisms, enzyme engineering, environmental microbiology,foodmicrobiologyandmolecularbiotechnology. LSM3243 MOLECULAR BIOPHYSICS (Elective, 4MC) Prerequisite:LSM1101 Workload:26lecturehours+24tutorialhours This module provides physical bases of macromolecular conformations and conformational stabilities under various conditions. This module also introduces basic principles and applications of a number of biophysical techniques that are commonly used for the studies of structure, dynamics and interactions of biomolecules.Specifictopicsincludeconformationofbiologicalmacromolecules,interactions(forces)that determine biomolecular structures, protein folding, proteinligand interaction, biological membrane, and biophysicaltechniques. LSM3244 MOLECULAR BIOTECHNOLOGY (Elective, 4MC) Prerequisite:LSM2102 Workload:26lecturehours+4tutorialhours+20laboratoryhours ThismodulehighlightstheapplicationsofrecombinantDNAandtransgenictechnologiestoproduceuseful biotechnology products (e.g.: molecular diagnostics, therapeutics, agrichemicals) or commercial processes in life science. Topics to be covered include: stateoftheart molecular tools and systems for biotechnology; recombinant protein production and downstream processing; transgenic plants and
7

animals as bioreactors for production of pharmaceuticals (e.g.: vaccines, antibodies); human molecular diagnostics, therapeutics and gene therapy; marine biotechnology; environmental biotechnology and its impact on human health; regulatory guidelines for biotechnology products and release of GMOs into the environment. LSM3252 EVOLUTION AND COMPARATIVE GENOMICS (Elective, 4MC) Prerequisite:LSM1103 Workload:26lecturehours+6tutorialhours+18practicalhours PresentationofthetheoryofEvolutionasoneoftheunifyingdisciplinesinbiology.Themoduleintroduces the three main themes in modern Evolutionary Biology: (1) TreeofLife: including processes and mechanisms of speciation, (II) Natural selection: including sexual selection and kinship selection, (III) Neutral Evolution: including genetic drift. Comparative Genomics is incorporated in the module by discussing the origin of life, whole genome comparisons, gene duplication, and using molecular data for the reconstruction of the phylogenetic relationships between species. The module emphasizes the importanceofevolutionarybiologyasatoolthatcanexplainawidevarietyofphenomenainbiology. LSM3254 ECOLOGY OF AQUATIC ENVIRONMENTS (Elective, 4MC) Prerequisites:LSM2251 Workload:26lecturehours+24tutorial/practicalhours Aquatic environments make up >70% of the Earths surface. They host a huge diversity of life and ecosystems, many of which are vital to man. Topics covered in this module include the diversity and ecology of freshwater and marine habitats and organisms, the impacts of humans on these environments, and the conservation and management of these critical resources. Overall learning outcomes include an appreciation and understanding of aquatic habitats, their physical and biological properties and their associated ecosystems. The importance of both marine and freshwater environments to Singapore will be highlighted. LSM3255 ECOLOGY OF TERRESTRIAL ENVIRONMENTS (Elective, 4MC) Prerequisite:LSM2251 Workload:26lecturehours+6tutorialhours+18practicalhours This module will introduce students to the principles of terrestrial ecology, with a focus on tropical forests in Southeast Asia. Major topics will include diversity and distributions of terrestrial vegetation types, soils and nutrient cycling, animalplant interactions (pollination, seed dispersal, herbivory), disturbance ecology and succession, energy flow and food webs, and population biology. Emphasis will be placed on how people are influencing these ecological processes through their activities (e.g. landuse changes, logging, hunting,fragmentation,climatechange). LSM3256 TROPICAL HORTICULTURE (Elective, 4MC) Prerequisite:LSM1103andLSM1104(Noteligibletoenrolwithoutpassingbothmodules) Workload:26lecturehours+18practicalhours+6tutorialhours This module introduces students to the fundamentals of tropical horticulture, with emphasis on the situation in Singapore, a tropical garden city. Topics include plant growth and development and factors affecting them, pests and diseases and their control, growing media, plant nutrition, tropical urban horticulture of ornamentals, vegetable and fruit crops, native ornamental plants, vertical and roof greening, turf grass management, landscape design, organic methods and impact of horticulture on conservation. Selfdirected field trips and projects will enable students to enjoy handson experience in tropicalhorticulture.

LSM3261 LIFE FORM AND FUNCTION (Elective, 4MC) Prerequisites: LSM1103 and either LSM1104 or LSM1201 (Not eligible to enrol without passing both modules) Workload:26lecturehours+6tutorialhours+18laboratoryhours This module explores the complex and diverse forms and functions of plants and animals. Each living group shares a common basic structural plan, but contains many members that deviate from the basic design in response to selection pressure. Knowledge of organismal biology is enhanced through selected topicsinmorphoanatomicaldesignsandfunctionaladaptations. LSM3262 ENVIRONMENTAL ANIMAL PHYSIOLOGY (Elective, 4MC) Prerequisite:LSM1104orLSM1201 Workload:26lecturehours+4tutorial(presentation)hours+20laboratoryhours Thismoduleaimstoexaminethephysiologicalandbiochemicaladaptationsofanimalswhichpermitthem to thrive in diverse environments. It focuses on how animals adapt to natural (e.g. oxygen availability, salinity changes, water availability) and anthropogenic (e.g. greenhouse effect, UV radiation and oxidative stresses,xenobiotics)environmentalchallenges.Thismodulehopestoofferstudentscluestowhatarethe fundamentalwaysinwhichbasicbiologicalstructuresandfunctionsoflivingsystemsareactivelymodified toallow organismstoexploitthefullrangeofnaturalenvironmentsandtomaintain theradicallydifferent modesoflifeweseeinnature.Effortswillbemadetoteachhowenvironmentalphysiologycanbeapplied tobiomedicine,agriculture,ecologyandenvironmentalconservationinthelastpartofthemodule. LSM3263 Field Studies in Neotropical Ecosystems (Elective, 4MC) Prerequisite:LSM2251 Workload:60lecturehours+24tutorialhours+36laboratoryhours+36hoursof fieldwork/projects/assignments+24preparatoryhours An intensive sixweek summer program conducted with the University of Costa Rica. The first two weeks will be spent on campus in San Jose, with lectures and tutorials on comparative paleotropical (Singapore) and neotropical (Costa Rica) terrestrial and marine ecosystems; comparative urban ecology as well as comparative conservation issues and policies. The latter four weeks will be spent at research stations locatedinthevariousecosystems;thecloudforest,neotropicalmontaneforest,tropicalCaribbeancoastal forest and coastal Pacific ecosystem. Students are exposed to different techniques in field biology and will be trained in forming ecological hypotheses while in the field. Lectures, student projects and student assessmentswillbecarriedoutateachfieldsite. LSM3272 GLOBAL CHANGE BIOLOGY (Elective, 4MC) Prerequisite:LSM1103 Workload:26lecturehours+24tutorial/practicalhours This module will introduce students to aspects of current environmental change and its effects on biological systems, including rising carbondioxide (CO2) concentrations, climate change, loss of biodiversity,andeutrophication.Topicscoveredwillincludethefollowing:climatechange,globalwarming, landuse changes, invasive species, fire, carbon sequestration, carbon mitigation and global ecology. Students will explore prevailing environmental issues in greater depth through mini projects, classroom discussionsanddebates. LSM4211 TOXICOLOGY (Elective, 4MC) Prerequisite:LSM3211orLSM3221 Workload:26lecturehours+16PBLhours+4Revisionhours Toxicologyisthestudyoftheadverseeffectsofchemicalsonhumans.Thisintroductorycoursewillprovide the basics for quantitative risk assessment of hazardous compounds and for extrapolation from animal datatohumans.Itwilllinkadverseeffectsatthemolecularleveltooveralltoxicresponsesinhumans.The
9

generalconceptswillbeillustratedwithexamplesincludingdrugs,naturaltoxins,industrialchemicals,and environmentaltoxicants. LSM4212 PHARMACOGENETICS AND DRUG RESPONSES (Elective, 4MC) Prerequisite:LSM3211orLSM3221 Workload:34lecturehours+15tutorialhours+1hrCA This module will examine the scientific bases for all aspects of human variability in clinical responses to drugs and other xenobiotics. The course will provide both the theoretical and technical knowhow to conduct and interpretsimple studies relating to intraindividual, interindividual as wellas interpopulational differencesindrugresponses. LSM4213 SYSTEM NEUROBIOLOGY (Elective, 4MC) Prerequisite:LSM3213 Workload:28lecturehours+10Tutorialhours+12SelfDirectedLearning(SDL)hours The primary goal of this module is to understand how (a) neurons, assembled into circuits, mediate behaviorand(b)thepathophysiologyofneuronsleadingtodysfunctionalcellularandmolecularprocesses andbehavior.Thiscoursedrawsonbasicknowledgeofthecellbiologyandphysiologyofneurons. LSM4214 CANCER PHARMACOLOGY (Elective, 4MC) Prerequisite:LSM3211andLSM4243(LSM4243canbereadascorequisite) Workload:lecturehours+tutorialhours This module will introduce students to the general principles of drug actions that underpin their therapeutic applications against cancers, from conventional (nonspecific) chemotherapy to targetspecific drugs.Itwillprovidedetailsofdrugsusedinspecificcancertypes,rangingfromthosewithprovenefficacy in clinics (e.g. Gleevec) to experimental agents in trials. Conceptual and theoretical targets (e.g. RNAi and genetherapies)willalsobeintroduced. LSM4221 DRUG DISCOVERY AND CLINICAL TRIALS (Elective, 4MC) Prerequisite:LSM3211orLSM3221 Workload:31lecturehours+6groupworkhours+9project/seminarhours+4selfdirectedlearninghours This module will cover the stages that a drug that is developed for clinical use goes through before it is marketed: discovery/synthesis, preclinical studies, clinical drug trials, registration and postmarket surveillance. The different phases of clinical drug trials and the guidelines for ethics and good clinical practice will be discussed. Students are also divided into groups to discuss and design clinical trials. At the endofthecoursethestudentswillhaveanoverview oftheprocessesinvolvedinbringingadrugfromthe laboratorytothemarket. LSM4222 ADVANCED IMMUNOLOGY (Elective, 4MC) Prerequisite:LSM3223 Workload:28lecturehours+22tutorialhours The objective of this course is to provide students with a current and up to date view of immunology. Breakthrough areas will certainly vary from year to year, but the broad subject matter will remain. Among the highly competitive areas of immunology research, the course focuses on innate immunity, dendritic cell biology, antigen processing and presentation, lymphocyte development and differentiation, induction oftolerance,mechanismofautoimmunityandallergy,hostpathogeninteractionsandtumorimmunology. LSM4223 ADVANCES IN ANTIMICROBIAL STRATEGIES (Elective, 4MC) Prerequisite:LSM3232orLSM3225 Workload:16lecturehours+8teampresentationhours+21practicalhours+5tutorial/consultationhours An advanced course in the study of infectious diseases of man with emphasis on new and emerging infections as well as those of major clinical/economic importance. Core topics include understanding the
10

principles and practice of Medical Microbiology, the nature and emergence of antimicrobial resistance, antimicrobial strategies, changing epidemiology of infections and laboratory diagnosis using classical diagnostic techniques and current molecular approaches. Seminars will be conducted as team presentations to explore current topics on infectious diseases in depth. A strong practical component is included. LSM4224 FREE RADICALS & ANTIOXIDANT BIOLOGY (Elective, 4MC) Prerequisite:LifeSciencesmajor Workload:26lecturehours+10presentationhours+14selfdirectedlearning/preparationhours This module examines the role of free radicals and antioxidants in human health and disease, with a focus on molecular and cellular aspects. Topics covered include free radical chemistry, antioxidant defences, their role in normal metabolism, cardiovascular and neurodegenerative diseases, cancer and the ageing process. At the end of this module students should be able to critically evaluate current literature in this area. LSM4225 GENETIC MEDICINE IN THE POSTGENOMIC ERA (Elective, 4MC) Prerequisite:LSM2102 Workload:27lecturehours+23presentation/selfdirectedlearning/preparationhours Thismoduleisintendedtoprovideagoodfoundationandstimulatestudentsinterestinspecializedtopics in Genetics and Genomics related to translational research. The module will provide students with knowledge of current practices in Genetic Medicine. Students will also know how gene identification, diagnostic and therapeutic strategies are formulated and performed. They will also be expected to show howtotranslatenewgeneticandgenomicdiscoveriesintonoveldiagnosticandtherapeuticstrategies. LSM4226 INFECTION AND IMMUNITY (Elective, 4MC) Prerequisite:LSM3223andLSM3232 Workload:38lecturehours+12tutorialhours This module aims at providing an indepth knowledge in the field of hostpathogen interactions, i.e. how theimmunesystemdealswithpathogens,andhowthepathogensdealwiththehostsimmunesystem.An introductory lecture series covers the basics in microbiology (bacteriology, virology, parasitology), immunology,vaccinology,andgeneralprinciplesofhostpathogeninteractions.Selecteddiseasesillustrate hostpathogensinteractionsalongwiththeconsequencesforvaccineanddrugdesign.Thefollowingsetof lecturescoveredbycliniciansandprofessionalsfocusonpatientmanagement,fieldstudy,aswellassafety aspects when working with pathogens in a research lab. Tutorials are broken into journal club, article writeup exercise and problembased study and are directly related to the topics developed during the lectures. LSM4231 STRUCTURAL BIOLOGY (Elective, 4MC) Prerequisite:LSM2103 Workload:28lecturehours+8practicalhours+14tutorialhours This module provides an overall view on the structure determination of protein molecules, protein complexes, and viral assemblies, and molecular architectures in cell. Topics will include the theory and practice of the three major methods cryo Xray crystallography, electron microscopy (cryoEM), and nuclearmagneticresonance(NMR). LSM4232 ADVANCED CELL BIOLOGY (Elective, 4MC) Prerequisite:LSM2103 Workload:26lecturehours+8tutorialhours+10journaldiscussionhours+6hoursfor2CAs,1report This module will explore the changes that occur in animal cells as they grow, mature, differentiate, and either senesce or renew themselves. Insights into the mechanisms that govern how and when particular
11

developmental alterations occur will be discussed. Emphasis will be placed on understanding the cellular molecularmechanismsthatlendthemselvestoexperimentalmanipulation. LSM4241 FUNCTIONAL GENOMICS (Elective, 4MC) Prerequisite:LSM3231 Workload:26lecturehours+24tutorialhours This module aims to introduce selected topics on functional genomics. Areas covered include: the assignment of functions to novel genes following the genomesequencing projects of human and other organisms; the principles underlying enabling technologies: DNA microarrays, proteomics, protein chips, structural genomics, yeast twohybrid system, transgenics, and aspects of bioinformatics and its applications;andtounderstandtheimpactoffunctionalgenomicsonthestudyofdiseasessuchascancer, drugdiscovery,pharmacogeneticsandhealthcare. LSM4242 PROTEIN ENGINEERING (Elective, 4MC) Prerequisite:LSM3244 Workload:26lecturehours+24hoursoftutorialanddiscussions This module will familiarize students with the technologies that can be used to produce and engineer various proteins for basic biological research and biotechnology applications. The fundamental principles for manipulating protein production as desired and the common expression systems will be presented. The emphasis will be on the experimental strategies and approaches to improve protein properties and to createnovelenzymaticactivities.Thetopicsincludegeneexpressionandproteinproductionsystems,uses of gene fusions for protein production and purification, directed molecular evolution and DNA shuffling, and engineering of proteins and enzymes for improved or novel properties. Some specific examples in proteinengineeringwillbehighlighted. LSM4243 TUMOUR BIOLOGY (Elective, 4MC) Prerequisite:LSM2103 Workload:32lecturehours+6hours(JournalDiscussions)+14hours(tutorials/studentassignments/CAs) Thismoduledealswiththeunderstandingofprocessesthatregulatecellgrowthandproliferation,andthe intricate mechanism(s) that result in abnormal proliferation and oncogenesis. Molecular basis of immortalization and the acquisition of the neoplastic phenotype, namely oncogene activation, immune evasion, potential for local and distant spread, and resistance to cell death etc. will be discussed. Role of DNA damage/repair, telomere/telomerase in genome instability and tumourigenesis will be examined. A brief session on target therapies including gene therapy approaches will also be included. Tumour immunologyroleofinflammationintumourswillbediscussed. LSM4251 PLANT GROWTH AND DEVELOPMENT (Elective, 4MC) Prerequisite:LSM3253orLSM3244 Workload:30hrlecturesand20hrstermpaperandpresentation Thismoduleaimstoprovideanoverallviewongrowthanddevelopmentofhighervascularplantsthrough their life cycles. Discussion in this module include selected topics in gamete development, fertilization, embryo development, seed germination, development of various plant organs and flowering, the role of plant growth regulators, and the cellular, physiological and molecular basis of plant morphogenesis. The moleculargeneticmechanismsofvariousstagesofplantdevelopmentwillbediscussed. LSM4252 ANIMAL REPRODUCTION (Elective, 4MC) Prerequisite:LSM3233 Workload:30hlectures+12htutorials/presentations+8hassignments/readings This module covers the events and mechanisms leading to the development and differentiation of gonads and sexes in vertebrates, and eventually to the reproduction and propagation of a new generation. It also
12

describes the use of animal models and discusses selected topics to highlight the current trends in animal reproduction. LSM4253 BEHAVIOURAL BIOLOGY (Elective, 4MC) Prerequisite:LSM2251orLSM3252 Workload:26lecturehours+24hourstutorial,discussions,groupprojectand/ortermpaper This module provides an indepth coverage of the relationships that organisms have with each other and with the environment. Selected key concepts in organismal interactions, illustrated with examples from general diverse animals and ecological systems, to ultimate and proximate explanations of animal interactionsandotherlifehistorycharacteristics,willbecovered.Studentswillbegiventheopportunityto assimilate and critically evaluate contemporary literature on relevant current issues. Experimental group field/labprojectswillbedesigned,proposedandcarriedoutbystudentstoimprovetheirunderstandingof animal behaviour and to appreciate the significance of behavior in ecology as well as other related disciplines. LSM4254 PRINCIPLES OF TAXONOMY AND SYSTEMATICS (Elective, 4MC) Prerequisite:LSM1103 Workload:lectures:2hrs/week;practicals/tutorials:4hrs/biweekly This module introduces students to taxonomy and systematics; i.e., the science of grouping biodiversity into species, describing the species, and classifying this diversity into higherlevel taxa that reflect evolutionary history. The module has two main goals: (1) It introduces the main concepts and goals of taxonomyandsystematics.(2)Itteachesthequalitativeandquantitativetechniquesthataretodayusedto describe/identify species and higherlevel taxa based on the analysis of morphological and DNA sequence evidence. The aim is to equip environmental as well as other biologists with a thorough understanding of taxonomic/systematic units and the tools needed for evaluating and quantifying diversity in samples of plantandanimalspecimens. LSM4261 MARINE BIOLOGY (Elective, 4MC) Prerequisite:LSM3254 Workload:26lecturehours+24tutorialhours This module builds on the fundamental material covered in LSM3254. Primary literature and firsthand accounts will be used to explore selected topics, such as specific groups of organisms or particular environments, in more depth. Guest speakers will be asked to present their current work and open discussion will be encouraged. The focus will be on tropical marine biology, with Singaporean examples wherever possible. Students can expect problembased learning, cooperative learning and small group work. LSM4262 TROPICAL CONSERVATION BIOLOGY (Elective, 4MC) Prerequisite:LSM2251 Workload:26lecturehours+24tutorial/Discussionshours Conservationandthelossofbiodiversityandnaturalecosystemsarecurrentlyregardedasoneofthemost pressing problems facing mankind. The course will highlight the impact of habitat loss on biodiversity and the basis for formulation of effective conservation management strategies. The course will also introduce students to the theory of current conservation biology as illustrated by applications in tropical areas, species conservation issues, ecological challenges, role of zoological gardens, legal challenges etc. Conservation of tropical biota, management of local and regional environmental problems, appreciation and consideration of the socioeconomic issues will also be treated. Conservation priorities and developmental needs at the national level will also be discussed, with emphasis on Singapore and SE Asia. The coursewill have guest lecturers from overseas as wellas managers and conservationplayers from the localenvironment.Itwillalsoinvolveaspecialroundtablediscussiononspecificconservationissues.
13

LSM4263 FIELD STUDIES IN BIODIVERSITY (Elective, 4MC) Prerequisite:ForLifeSciencesHonoursstudentsorbydepartmentalapproval Workload:12lecturehours+8fieldpracticalhours+7dayfieldcourse LSM4263 will introduce students to field biology, conservation issues, the basic techniques involved, sampling design and basic data gathering and data management. From field practicals, students will experience and encounter tropical environs and habitats, namely coastal, mangrove, primary and secondary forest. A 7 day field course is incorporated and will be conducted in Pulau Tioman, Malaysia. There students, who will be divided into small groups, will conduct either 4 miniprojects in 4 separate habitats or one major project in a chosen habitat, under the supervision of experienced fieldorientated teaching assistants. This module will involve overseas university students as well as NUS Life Science students. LSM4264 FRESHWATER BIOLOGY (Elective, 4MC) Prerequisite:LSM3254 Workload:26lecturehours+24tutorial/discussion/fieldtriphours Freshwater is essential to life, yet constitutes less than 3% of Earths total water. With many freshwater ecosystems under threat, understanding the biology of freshwaters is fundamentally important to their management, conservation, and restoration. This module introduces the study of inland waters. Through lectures, directed readings and discussions, field trips, and project work, we will focus on topical areas includingaquaticbiodiversityandecology,aquaticconservation,freshwaterecosystemservices,threatsto freshwaters, anthropogenic effects, sustainable water use, aquatic invasive species, and policies, regulationandmanagementoffreshwaterresourcesinlocalandinternationalcontexts. LSM4265 URBAN ECOLOGY (Elective, 4MC) Prerequisite:LSM2251andLSM3255 Workload:26lecturehours+24practicalhours This module introduces students to the ecology of urban areas, with a focus on tropical cities. It will deal with terrestrial, freshwater and coastal marine environments in which urbanization is the key ecological factor. Topics covered will include the origins of cities, urbanization as a process, urban landscapes, urban environments(soils,hydrology,climatesandpollution),urbanbiodiversity,alienspecies,landscapedesign, urban greenery, pest and vector control, ecological footprints, and the sustainable city. Students will undertake a smallgroup research project involving the design, implementation, analysis and presentation ofanurbanecologystudy.
Disclaimer: Contents of this handbook are correct at the time of printing but updates happen regularly and it is recommended to check the Life Sciences website for updates.

14

SEMESTER I & II LSM1101 BIOCHEMISTRY OF BIOMOLECULES

Prerequisite: GCE A level Biology or H2 Biology or equivalent or LSM1301 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This module provides an overview of pH and buffers, and the physical-chemical basis of biological molecules: nucleic acids, proteins, carbohydrates and lipids; their structure-function relationships. Enzymes, enzyme kinetics and applications of enzymes, heme biosynthesis and metabolism will also be covered. S/N 1. Topics pH and buffers Dissociation of water and weak acids/bases, pKa values Henderson-Hasselbach equation Buffers (including physiological buffers) Amino acids and proteins Amino acids : structure and classification; titration curves Proteins: primary, secondary, tertiary, and quaternary structures; structure function relationships, denaturation. Enzymes Concept of catalysis, isoenzymes Enzyme kinetics and inhibition Application of enzymes Carbohydrates Mono, di-, oligo-, and polysaccharides. Physiologically important carbohydrates Lipids Classification and functions, chemistry of lipids; membrane lipids 5. Nucleic acids Chemical structures of bases, nucleosides and nucleotides, cyclic nucleotides; structure, function and properties of DNA and RNA 6 Heme Biosynthesis and catabolism, heme-containing proteins and their biological functions Lecture hours 3 Takao Inoue/ Too HP

2.

5 Takao Inoue/ Too HP 5 Deng Lih Wen 4 K Swaminathan 4 K Swaminathan 2 K Swaminathan 3 Deng Lih Wen

3.

4.

Total Lectures: 26h Tutorials: 6h Practicals: 6x3= 18h CA = 2h Total hours: 50h

TEXT BOOK :Garrett & Grisham. Biochemistry (4th Ed); Voegt, Voegt & Pratt. Fundamentals of Biochemistry (2nd Ed). MODULE CO-ORDINATOR: Semester I Dr Deng Lih Wen Semester II A/P Too Heng-Phon LECTURERS: Dr Deng Lih Wen (Sem I & II) A/P K. Swaminathan (Sem I & II) A/P Too Heng-Phon (Sem II) Dr Takao Inoue (Sem I) (Tel: 6516-1239; E-mail: bchdlw@nus.edu.sg) (Tel: 6516-3687; E-mail: bchtoohp@nus.edu.sg)

(Tel: 6516-1239; E-mail: bchdlw@nus.edu.sg) (Tel: 6516-7932; E-mail: dbsks@nus.edu.sg) (Tel: 6516-3687; E-mail: bchtoohp@nus.edu.sg) (Tel: 6516-2204; E-mail: bchti@nus.eedu.sg)

15

SEMESTER I & II LSM1102 MOLECULAR GENETICS

Prerequisite: GCE A level Biology or H2 Biology or equivalent or LSM1301 Workload: 26 lecture hours + 24 practical and tutorial hours The course will cover topics on (i) the patterns of inheritance, (ii) the molecular structure and replication of genetic material, (iii) the molecular properties of genes, and (iv) genetic analysis of individuals and populations. This will include an in-depth understanding of Mendelian patterns of inheritance and variations that could occur due to multiple alleles, lethal genes, chromosomal variations, linkage, gene interaction and other genetic phenomena. Emphasis is placed on the understanding of the underlying molecular and biochemical basis of inheritance. Quantitative and population genetics will also be discussed with the emphasis of understanding the processes and forces in nature that promote genetic change. S/N 1. Topics (1) Overview of Genetics and Cellular Organization (2) Reproduction and Chromosome Transmission (Cellular Division: Mitosis and Meiosis) (3) Genetic transfer and mapping analysis in microorganisms (4) Chromosome organization, molecular structure and recombination Continual Assessment 1 (on Lectures 1-4) (5) Molecular structure of DNA and RNA (6) DNA replication (7) Gene transcription and RNA processing (8) Translation of mRNA Continual Assessment 2 (on Lectures 5-8) (9) Mendelian genetics + Sex Chromosomes and Sex Linkage (10) Modes of Inheritance and Pedigree Analysis (11) Variations to Mendelian Genetics, Penetrance, Expressivity, Pleotrophy and Linkage (12) Population Genetics Hardy-Weinberg Equilibrium, Mutation and Selection, Fitness, Factors Maintaining Polymorphism e.g., Overdominance (13) Quantitative Genetics Continual Assessment 3 (on Lecture 9 onwards) Lecture hours

8 Wu Jinlu (Sem 1 & 2) 8 He Yuehui (Sem 1) Liou Yih-Cherng (Sem 2) 10 Chew Fook Tim (Sem 1 & 2)

2.

3.

Total Lectures: 26h Practicals: (4x3)= 12h Tutorials & Continual Assessments: 12h Total hours: TEXT BOOK (RECOMMENDED): Genetics. Analysis & Principles by R J Brooker. Addison Wesley / McGraw-Hill Principles of Genetics by Snustad & Simmons. John Wiley MODE OF ASSESSMENTS: 60% Continual Assessments/Class Tests/Assignments/Quizzes/Lab Sessions/Reports/Attendance 40% Semester Exam MODULE CO-ORDINATORS: A/P Chew Fook Tim LECTURERS: Semester I Dr Wu Jinlu Dr He Yuehui A/P Chew Fook Tim Semester II Dr Wu Jin Lu Dr Liou Yih-Cherng A/P Chew Fook Tim (Tel: 6516-1685, E-mail: dbscft@nus.edu.sg) 50h

(Tel: 6516-8476, E-mail: dbswjl@nus.edu.sg) (Tel: 6516-2716, E-mail: dbshy@nus.edu.sg) (Tel: 6516-1685, E-mail: dbscft@nus.edu.sg) (Tel: 6516-8476, E-mail: dbswjl@nus.edu.sg) (Tel: 6516-7711, E-mail: bchlyc@nus.edu.sg) (Tel: 6516-1685, E-mail: dbscft@nus.edu.sg)

16

SEMESTER I & II LSM1103 BIODIVERSITY

Prerequisite: GCE A level Biology or H2 Biology or equivalent or LSM1301 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This course provides life science majors with a critical introduction to the diversity of life on earth. The means to identify and differentiate major groups of living is emphasised. Unique characteristics about the life history and ecology of these groups will be discussed through local and regional examples and field trips will provide an opportunity to explore the biodiversity of selected ecosystems around Singapore. This foundation in biodiversity integrates conservation issues such as the biodiversity crisis to provide an understanding of the need for a diverse and intricate balance of nature. S/N Topics Introduction to biodiversity, classification and nomenclature Issues in biodiversity conservation MICROBIOLOGY Introduction to microbial systematics Bacteria I: Gram negative bacteria Bacteria II: Gram positive bacteria BOTANY Protista (photosynthetic), fungi, and lichen Lower plants Higher plants ZOOLOGY Introduction to the Animal Kingdom & Non-photosynthetic Protists; Invertebrates I: Sponges and cnidarians Invertebrates II: Worms (annelids and platyhelminthes) and mollusks Invertebrates III: Arthropods, echinoderms, and protochordates Vertebrates I: Fishes, amphibians and reptiles; Vertebrates II: Birds and mammals Total lecture hours: Practicals (18 hours) + Tutorials (6 hours) TOTAL Lecture hours 2 6

I.

II.

III.

12

IV.

26 24 50 hours

TEXT BOOK (Recommended texts only): Microbiology: 1. Microbiology. 7th Edition (2008) by JM Willey et al. McGraw-Hill Plants and Animals: 2. Biology. 9th Ed., 2010. By Eldra Solomon, Linda Berg & Diana W. Martin. 1234pp. Thomson Brooks/Cole OR Campbell Biology. 9th Ed., 2010. By J. B. Reece et al. 1464pp. Pearson/Benjamin Cummings. 3. Integrated Principles of Zoology. 15th Ed, 2010. By Cleveland Hickman, Jr., Larry Roberts, Susan Keen, Allan Larson, Helen I'Anson, & David Eisenhour. 928pp. McGraw-Hill. MODE OF ASSESSMENT: 1. 50% Continual Assessment: tests, essays and a practical test. 2. 50% Final Examination (Open Book) MODULE COORDINATORS/LECTURERS (Zoology): Mr N. Sivasothi (Semester I) (Tel: 6516-8869, E-mail: sivasothi@nus.edu.sg) Dr Ng Ngan Kee (Semester II) (Tel: 6601 1091, E-mail: dbsngnk@nus.edu.sg) CO-LECTURERS (Microbiology & Botany), Semesters I & II: A/P Ho Bow (Microbiology) (Tel: 6516-3285, E-mail: michob@nus.edu.sg) A/P Benito Tan (Botany) (Tel: 6516-2858, E-mail: dbsbct@nus.edu.sg)

17

SEMESTER I & II LSM1104 GENERAL PHYSIOLOGY

Prerequisite: GCE O Level Biology or LSM1301 Workload: 26 lecture hours + 2 CA hours + 4 tutorial hours + 18 laboratory hours This module deals with General Physiology and its theme is Biological Transducers and Energy Transformation. This module will start with bioenergetics which stresses on the application of thermodynamics to physiological processes in both animals and plants. Six types of energy will be dealt with, concerning (1) the transformation of light energy to chemical energy by plants, (2) the transformation of chemical energy to chemical potential energy of ions and water across bio-membranes, (3) the transformation of chemical potential energy to electrical energy by plasmalemma with special emphasis on neurons, (4) the transformation of chemical energy to mechanical energy during animal locomotion, and (5) and the production and release of heat during energy transformation. In this module, the important concept of homeostasis, with special emphasis on the balance of heat gain and heat loss to maintain a constant body temperature will be covered.

S/N 1.

Topics a. Photosynthesis: from light to chemical energy b. Water and solute transport: ATP and chemical potential energy c. Water flux in plants a. Food and energy intake in animals b. Cellular respiration: production of cellular chemical energy (ATP) a. Diffusion, facilitated diffusion and active transport: from chemical energy to chemical potential energy b. Ionic gradients and membrane potential: from chemical potential energy to electrical energy c. Neural signals a. Cilia, flagella and amoeboid movement: from chemical energy to mechanical energy b. Muscle contraction

Lecture hours 10 P Kumar/ B. L. Ong 4 Y. K. Ip 6

2. 3.

4.

6 Y. K. Ip

Total Lectures:26h CA : 2h Tutorials: 4h Practicals: 6x3= 18h Total hours: 50h

REFERENCE BOOKS: R Garrett and C M Grisham, Molecular Aspects of Cell Biology (Saunders College Publishing, New York, 1995) D Randall, W Burggren and K French, Animal Physiology, 4th Edition (W H Freeman and Company, New York, 1997) W. G. Hopkins and N. P.A. Hner. Introduction to plant physiology. 4th Edition. (Hoboken, N.J. : Wiley, 2009) MODULE CO-ORDINATOR: Prof Ip Yuen Kwong LECTURERS: Prof Ip Yuen Kwong Prof Prakash Kumar Dr Ong Bee Lian (Tel: 6516-2702, E-mail: dbsipyk@nus.edu.sg) (Tel: 6516-2702, E-mail: dbsipyk@nus.edu.sg) (Tel: 6516-2859, E-mail: dbskumar@nus.edu.sg) (Tel: 6516-2852, E-mail: dbsongbl@nus.edu.sg)

18

SEMESTER II LSM1202 HUMAN ANATOMY

Prerequisite: GCE 'O' level Biology or LSM1301 Workload: 26 lecture hours + 22 laboratory hours This module provides a basic introduction to human structure and function, comprising gross anatomy integrated with microscopic anatomy. Histological organization of the primary tissues: epithelial, connective, muscular and nervous tissues will also be covered. Clinical relevance of the anatomical structures will be discussed. S/N 1. 2. 3 4 5 6 7 8 9 10 11 Cells and Tissues of the Body Musculoskeletal System Respiratory System Cardiovascular System Blood Digestive System Urinary System Reproductive System Immune System Endocrine System Nervous System Topics Lecture hours 2 He BP 2 Ng YK 2 Ng YK 2 Ng YK 2 He BP 4 Ang ET 2 He BP 2 Ang ET 2 He BP 2 Ang ET 4 Ng YK Total Lectures: 26 hrs Practicals: 22 hrs Total hours: 48 hrs

TEXT BOOKS: Human of Anatomy, Edition 6, 2008, Author: Frederic H. Martini, Michael J. Timmons, and Robert B. Tallitsch Publisher: Pearson MODE OF ASSESSMENT: 2 Continual Assessments (in the form of multiple choice questions (CA1) and short essay questions (CA2) and 1 Final Examination paper (in the form of multiple choice questions and short essay questions) 30% CA, 70% Final Examination MODULE CO-ORDINATOR: Dr He Beiping LECTURERS: A/P Ng Yee Kong Dr Ang Eng Tat (Tel: 6516-3689, E-mail: antngyk@nus.edu.sg) (Tel: 6516-8110, E-mail: antaet@nus.edu.sg) (Tel: 6516-7809, E-mail: anthebp@nus.edu.sg)

19

SEMESTER I, II & IV LSM1301 GENERAL BIOLOGY

Preclusion: Must not have read GCE 'A' Level or H2 Biology, or equivalents. Cannot be read concurrently with LSM1101, LSM1102, LSM1103, LSM1104 and LSM1202. 'A' Level equivalents include: American High School Diploma Australian High School (Australian Capital Territory Exam, New South Wales HSC, Queensland Senior Certificate, South Australian Matriculation, Victoria Certificate of Education, Western Australia Year 12) Bangladeshi HSC Canadian Pre-U (CPU) European Baccalaureate Filipino High School French Baccalaureate (FB) Diploma German Abitur Hong Kong Diploma of Secondary Education (HKDSE) Indian Standard 12 (Central, ISCE, State, and other boards) Indonesian SMU UAN (Ebtanas) International Baccalaureate (IB) Diploma Mauritius HSC Mathayom 6 (Thai High School) Myanmar High School New Zealand University Entrance, Bursaries and Scholarship Examination or National Certificate of Educational Achievement Level 3 NUS High School Diploma Ontario Secondary School Diploma (OSSD) Pakistani Intermediate PRC National College Entrance Examination or Gao Kao Sijil Tinggi Persekolahan Malaysia (STPM) Turkish High School Unified Examination Certificate (UEC) Vietnamese High School Graduation Examination Workload: 24 lecture hours + 26 tutorial hours (practical sessions, assignments, and museum visit) This is an introductory module that explores what a living thing is, the basics of life, and the science behind it. The course will introduce the chemistry of life and the unit of life. The question of how traits are inherited will be discussed and the field of biotechnology, including its applications and the ethical issues involved will be introduced. The diversity of life on earth will be explored, with discussions on how life on earth possibly came about and how biologists try to classify and make sense of the diversity. The course will also introduce the concept of life functions from cells to tissues and from organs to systems. The concept of how organisms maintain their internal constancy and organisation of major organ systems will be discussed. The focus will be to introduce the unifying concepts in biology and how they play a role in everyday life. S/N 1 2 3 4 5 6 7 Topics Science of Biology What is a living thing? What is the science of biology? What are the limits of science? Chemistry of Life Carbon compounds in cells carbohydrates, lipids, amino acids, and nucleic acids. Cell Structure and Function Features of eukaryotic and prokaryotic cells. Energy and Life Glycolysis. Citric acid cycle. Oxidative phosphorylation. DNA and Heredity DNA structure and replication. Gene Expression From DNA to proteins. Mutations and control mechanisms. Biotechnology DNA recombination and genetic engineering. Applications and implications of biotechnology.
20

Lecture Hours 2 2 2 2 2 2 2

S/N 8 9 10 11 12

Topics

Lecture Hours

Biodiversity Identification, naming, and classifying of organisms. How many species 2 are there? Plant Form and Function 2 Types of plant tissues. Plant nutrition and transport. Animal Form and Function 2 Animal tissues and organ systems. Example of an animal organ system. Ecology 2 Community interactions. Food chain and energy flow. Evolution 2 Principles of evolution. Evidence for natural selection. Lectures: 24 h Tutorials (Practical Sessions, Assignments, and Museum Visit): 26 h Total Hours: 50h

TEXTBOOKS: 1. Biology by Sylvia S. Mader, 10th Edition, 2010, McGraw-Hill Higher Education. 2. Biology: Concepts and Investigations by Marielle Hoefnagels, 2nd Edition, 2012, McGraw-Hill Higher Education. 3. Biology: Life on Earth with Physiology by Gerald Audesirk, Teresa Audesirk, and Bruce E. Byers, 9th Edition, 2011, Pearson Prentice Hall. 4. Biology: The Unity and Diversity of Life by Cecie Starr, Ralph Taggart, Christine Evers, and Lisa Starr, 12th Edition, 2009, Thomson Brooks/Cole. MODE OF ASSESSMENT: MODULE CO-ORDINATORS: Dr Seow Teck Keong A/P Loh Chiang Shiong Dr Wu Jinlu LECTURERS: Semester I Dr Seow Teck Keong Dr Zeehan Jaafar Semester II A/P Loh Chiang Shiong Dr Wu Jinlu Semester IV A/P Loh Chiang Shiong Dr Wu Jinlu 40% Continual Assessment + 60% Final Examination (Tel: 6516-2695, E-mail: teckeong@nus.edu.sg) (Tel: 6516-2916, E-mail: dbslohcs@nus.edu.sg) (Tel: 6516-8476, E-mail: dbswjl@nus.edu.sg)

(Semester I) (Semester II) (Semester IV)

(E-mail: teckeong@nus.edu.sg) (E-mail: dbszj@nus.edu.sg) (E-mail: dbslohcs@nus.edu.sg) (E-mail: dbswjl@nus.edu.sg) (E-mail: dbslohcs@nus.edu.sg) (E-mail: dbswjl@nus.edu.sg)

21

SEMESTER I, II & IV LSM1302/GEK1527 GENES AND SOCIETY

Preclusion: Not for students taking Life Sciences major/minor, FST, Pharmacy and Bioengineering major. Workload: 26 lecture hours + 24 hours of Tutorials, Interactive Learning & Feedback for CAs (CD-ROMassisted Learning, Web-based Interactive Learning, Assignments, Quizzes, etc.) The aim of the module is to introduce students to the modern concepts in biology and to enable them to evaluate independently the potential benefits and risks of the biotechnological revolution and its implications for society. It focuses on how genes and our understanding of it have impacted, are and will be impacting our lives as individuals and our society at large. While the module covers broad themes of genetics and biotechnology including related ethical issues and implications, it also provides sufficient grounding of fundamental concepts to ensure students of diverse backgrounds can appreciate the significance of past, present and future developments of genetic knowledge on societies. S/N 1. Topics Introduction: Historical and the Fundamentals An overview of how our understanding of genes have developed and changed over time, moving on from classical genetics (traits and inheritance) to laying down basic modern molecular biology concepts such as DNA structure, replication, transcription, translation, mutation to regulation and organization of genetic materials in different organisms. Lecture hours 10

2.

Tools in Genetic Engineering: From Laboratory to Society Principle description of how several essential techniques (e.g. nucleic acid detection, amplification, sequencing, cloning, gene transfer) are developed based on concepts laid in part (1) above and subsequently used for various real-world applications (e.g. forensic science, archaeology, health-care, drug discovery and even in art). Genetic Engineering Applications in Society: Microorganisms, Plants, Animals and Humans Specific real-world examples of genetic engineering applications in different organisms (microbes, plants, animals and humans) together with their benefits ( e.g. increase quality/quantity food production, new energy resources, better health-care) and potential harms (e.g. ecological and biodiversity impact, bio-terrorism) are highlighted. Ethical, Legal and Social Implications Ethical, legal and social implications of biotechnology applications in society are discussed with hypothetical and real-world examples to give students a better appreciation of the complexity and the diverse societal response to current and potential issues.

3.

4.

Total Lectures: 26h Tutorials, Interactive Learning & Feedback for Continuous Assessments: 24h
(CD-ROM-assisted Learning, Web-based Interactive Learning, Quizzes, Assignments, etc.)

Total hours:

50h

REFERENCES: 1. William J. Thieman and Michael A. Palladino. Introduction to Biotechnology. Pearson Benjamin Cummings, San Francisco. 2. Relevant state-of-the-art articles in popular press (e.g. Time, Newsweek) and popular scientific journals (e.g. Scientific American, New Scientist), as will be listed on the course website or provided during the lectures.

22

MODE OF ASSESSMENT: Continual Assessment (assignments, short structured questions, essay, quizzes, etc.): 40% Closed-Book Final Examination (Multiple Choice Questions): 60% MODULE CO-ORDINATORS: Dr Lam Siew Hong Assoc Prof K Swaminanthan LECTURERS: Dr Lam Siew Hong Assoc. Prof K Swaminanthan (Tel: 6516-7379, E-mail: dbslsh@nus.edu.sg) (Tel: 6516-7769, E-mail: dbslinqs@nus.edu.sg) (Tel: 6516-7379, E-mail: dbslsh@nus.edu.sg) (Tel: 6516-7932, E-mail: dbsks@nus.edu.sg)

(Semester I and IV) (Semester II)

(Semester I and IV) (Semester II)

23

SEMESTER II LSM1303 ANIMAL BEHAVIOR

Preclusion: Not for students with GCE A level Biology or H2 Biology or have passed LSM1301 or Life Sciences major/minor or Food Sciences & Technology or Pharmacy majors or Bioengineering program. Workload: 26 lecture hours + 4 tutorial hours + 6 laboratory hours + 14 group project field trip hours An understanding of an animal behavior provides an appreciation of the uniqueness of a particular species and other similar species. How an animal's behavior is best suited to a particular ecological situation is explored through fundamental and current theories including optimality, natural and sexual selection. A diversity of animal behaviors are explored such as learning, communication, territoriality and courtship. Research methods will also be discussed and explored through student projects. The knowledge gained through animal behavior can be translated into better animal care and conservation, and perhaps provide a better insight into our own behavior. S/N 1. Topics Overview & Introduction Overview of course structure Introduction: why animal behavior is studied and a brief history of the study of animal behavior Fundamentals of Animal Behaviour The science of behaviour (ethology) & Methods of observation Learning: types, e.g. habituation and trial-and-error learning Instinctive and learnt behaviours (simple stimuli) and Learning (complex stimuli) Natural selection; Nature versus Nurture Human/animal mate selection Diversity of Animal Behaviour Adaptation and decision making: theories of optimality, Living in groups & Social behaviour Animal communication: diversity and applications Territoriality Courtship & Mating Adaptations to urbanisation & Human - animal conflicts Lecture hours 2

2.

10

3.

14

Total Lectures: 26h Tutorials: 6h Practicals: 6x3= 18h Total hours: 50h

TEXT BOOK: The lecture series is not based on any specific text. Any basic animal behaviour book will address the topics covered, e.g. Mannning, A. & M. S. Dawkins, 1998. An Introduction to Animal Behaviour. Cambridge University Press, 5th Edition. 460p. Drickamer, L. C., S. H. Vessey & E. Jakob, 2001. Animal Behavior. McGraw-Hill Publishing Co., 5th Edition. 480p. MODE OF ASSESSMENT: 50% Continual Assessment Review, Blog post, Group Project 50% Final Examination (Closed Book) MODULE CO-ORDINATOR/LECTURER: Mr. N. Sivasothi (Tel: 6516-8869, E-mail: sivasothi@nus.edu.sg) CO-LECTURER: Dr. Zeehan Jaafar Technologist-in-charge: Mr. Tommy Tan (Tel: 6516-6867; E-mail: dbszj@nus.edu.sg) (Tel: 6516-2969, E-mail: dbstant@nus.edu.sg)

24

SEMESTER I & II LSM1401 FUNDAMENTALS OF BIOCHEMISTRY

Prerequisite: For Bioengineering, Chemical Engineering, Engineering Science Programme, Environmental Science and Engineering, Chemistry and Pharmacy students only. Pass in GCE 'A' Level or H2 Chemistry or equivalents. Preclusion: Not for Life Sciences major/minor (except Chemistry major) and student must not have read LSM1101 or its equivalent. Workload: 28 lecture hours + 10 tutorial hours (assignments, classes, etc.) + 3 laboratory sessions This module aims to provide the student with a strong background in the fundamental aspects of the biochemistry of biomolecules including selected topics of cell biology, microbial systems, and molecular genetics with an emphasis on their application to chemical and pharmaceutical industries as well as engineering practices (in particular bioengineering, chemical engineering, environmental engineering, and engineering science). Upon completing this module, the student is expected to have sufficient knowledge of fundamental life processes in order to appreciate and relate the importance of biochemistry in industry as well as in everyday life. The student should also be well prepared to take other advanced modules as well in which biochemistry is a prerequisite. S/N Topics Life and Aqueous Solutions Chemical processes as an integral part of a living cell. Acid-base reactions and concept of buffering. pH and buffers maintenance of biological systems in engineering and pharmaceutical processes. Cell Biology Diversity of living systems. Organisation, structure and functions of cells prokaryotes, eukaryotes, plant, and animal cells. Cell membrane and transport. Cell cycle. Cell growth and nutritional needs. Overview of growing cells batch and continuous culture. Industrial uses of microbes and other cell types. Biomolecules Protein Structure and Function Amino acids, peptides, and proteins. Chemical bonds and functional groups. Proteins as enzymes. Enzyme properties and kinetics. Industrial applications of proteins and enzymes. Immobilisation of proteins and enzymes. Biomolecules Lipid Structure and Function Structural diversity and functions. Lipids of commercial importance. Biomolecules Carbohydrate Structure and Function From monomers to polymers. Energy for human and raw material for industries. Cellular Metabolism Concept of metabolic pathways. Diversity of metabolism. Production and utilisation of energy. Energy from lipids (-oxidation). Glycolysis as model metabolic pathway. Fermentation. Citric acid cycle. Intermediary metabolism large scale production of specific metabolites. Biomolecules Nucleic Acid Structure and Function Structure and function of nucleic acids. Flow of genetic information replication, transcription, and translation. Manipulation and application of genetic information. Industrial application of recombinant DNA. Lectures: 28h Tutorials (Assignments, Classes, Etc.): 10h Laboratory Sessions: 3 4h = 12h Total Hours:
25

Lecture Hours 2

4 5

2 2

50h

TEXTBOOKS: 1. Biochemistry by Mary K. Campbell and Shawn O. Farrell, 6th Edition, 2009, Thomson Brooks/Cole. 2. Cell Biology and Genetics by Cecie Starr, Ralph Taggart, Christine Evers, and Lisa Star, 12th Edition, 2009, Thomson Brooks/Cole. 3. Biochemistry: The Molecular Basis of Life by Trudy McKee and James R. McKee, 3rd Edition, 2003, McGraw-Hill Higher Education. 4. Biochemistry by Jeremy M. Berg, John L. Tymoczko, and Lubert Stryer, 6th Edition, 2007, W.H. Freeman. 5. Molecular Cell Biology by Harvey Lodish, Arnold Berk, Chris A. Kaiser, Monty Krieger, Matthew P. Scott, Anthony Bretscher, Hidde Ploegh, and Paul Matsudaira, Sixth Edition, 2008, W.H. Freeman. 6. Principles of Biochemistry by Donald Voet, Judith G. Voet, and Charlotte W. Pratt, 3rd Edition, 2008, John Wiley. 7. Biochemistry by Reginald H. Garrett and Charles M. Grisham, 4th Edition, 2010, Thomson Brooks/Cole. 8. Lehninger Principles of Biochemistry by David L. Nelson and Michael M. Cox, 5th Edition, 2009, W.H. Freeman. MODE OF ASSESSMENT: MODULE CO-ORDINATORS: Dr Kim Chu-Young Dr Seow Teck Keong LECTURERS: Semester I Dr Kim Chu-Young Dr Lam Siew Hong Semester II Dr Lam Siew Hong Dr Seow Teck Keong 60% Continual Assessment + 40% Final Examination (Tel: 6516-1681, E-mail: dbskcy@nus.edu.sg) (Tel: 6516-2695, E-mail: teckeong@nus.edu.sg)

(Semester I) (Semester II)

(Tel: 6516-1681, E-mail: dbskcy@nus.edu.sg) (Tel: 6516-7379, E-mail: dbslsh@nus.edu.sg) (Tel: 6516-7379, E-mail: dbslsh@nus.edu.sg) (Tel: 6516-2695, E-mail: teckeong@nus.edu.sg)

26

SEMESTER I CM1401 CHEMISTRY FOR LIFE SCIENCES

Prerequisite: GCE A level or H2 Chemistry or equivalent or CM1417 Workload: 39 lecture hours + 13 tutorial hours + 15 laboratory hours This is a chemistry module catered for Life Science students and deals primarily with the basic principles to understand the structure and reactivity of organic molecules, basic thermodynamics and kinetics, and some basic analytical techniques. Students should acquire fundamental knowledge in chemistry for applications to biological systems. Lecture Topic Topic Description 1-2 Structure Atomic structure: wave-particle duality of matter and energy, electron configuration and chemical periodicity, exclusion principle, periodic table and its trends 3-4 Structure Models of chemical bonding: Lewis electron dot symbols, shapes of molecules, theories of covalent bonding - valence bond theory, hybrid orbitals, molecular orbital theory, electron delocalization. 5-6 Thermodynamics Laws of Thermodynamics, Gibbs energy. 7-8 Thermodynamics Chemical and acid-base equilibria 9 Electrochemistry Redox reactions, Nernst, ions in solutions 10 Kinetics Rates of reactions, temperature dependences, differential/integration methods in kinetics, collision theory, mechanisms 11-12 Analytical Spectroscopic techniques : UV, IR, NMR, mass spectrometry 13-14 Alkanes Nomenclature, properties, conformation, cycloalkanes, conformations of cyclohexane 15 Alkenes and Alkynes Nomenclature, electronic structure, addition reactions, carbocation structure and stability, oxidation of alkenes, preparation of alkenes, alkyne acidity 16 Aromatic compounds Structure and stability of benzene, electrophilic aromatic substitution, substituent effect in electrophilic aromatic substitution, xodiation and reduction of aromatic compounds 17-18 Stereochemistry Chirality, optical activity, specific rotation, enantiomers, diastereomers, meso compounds, molecules with more than 2 stereocentres, racemic mixture and the resolution of enantiomers 19 Alkyl halides Nomenclature, preparation of alkyl halides, Grignard reagents, SN1 and SN2 reactions, E1 and E2 reactions 20 Alcohols, ethers and phenols Nomenclature, properties, synthesis and reactions of alcohols, phenols and ethers, epoxides, ring-opening reactions of epoxides 21 Carbonyl compounds Nomenclature, structure and properties, synthesis of aldehydes and ketones, oxidation, nucleophilic addition reactions 22-23 Carboxylic acids and its derivatives Nomenclature, acidity of carboxylic acids, synthesis and reactions of carboxylic acids and their derivatives 24 Amines Nomenclature, structure and properties, amine basicity, synthesis and reaction of amines RECOMMENDED TEXTBOOK: Chemistry 5th Edition by Silberberg Fundamental Organic Chemistry 7th Edition by McMurry MODE OF ASSESSMENT: Tests, Lab and One Final Exam LECTURERS: Dr Lu Yixin Dr Emelyn Tan (Tel: 6516-1569 E-mail: chmlyx@nus.edu.sg) (Tel: 6516-2665 E-mail: chmtsqe@nus.edu.sg)
27

SEMESTER I & II ST1232 STATISTICS FOR LIFE SCIENCES

Prerequisite: GCE AO level or H1 pass in Mathematics or its equivalent Workload: 39 lecture hours + 11 tutorial hours An introduction to the basic principles and methods of biostatistics designed specifically for life sciences students who wish to learn modern research methods for analyzing and extracting information from biological, biomedical and genomic data. Relevant examples will be used throughout the course to illustrate various techniques. Two computer packages will be used to enhance learning and to enable students to analyze real life data sets. Topics include tabular and graphical display of data, probability and probability distributions, confidence intervals, hypothesis testing, distribution free tests, categorical data analysis, and regression analysis. S/N 1. 2. Topics Introduction to Applied Statistics in biomedical research Exploratory data analysis Tabular EDA Numerical EDA Graphical EDA Probability and Bayes Theorem Combination and permutation Conditional independence and mutually exclusivity Bayes Theorem Probability in genetics Probability distributions Random variables Discrete probability distributions Continuous probability distributions Confidence intervals Estimation and uncertainty Standard error Interpretation of confidence intervals Hypothesis testing Type I and type II errors P value Power and sample size determination Parametric tests for comparing means Categorical data analysis Test of goodness-of-fit Test of independence Non-parametric tests Linear regression and correlation Linear modeling Assumptions of linear regression Model selection and assessment Logistic regression Hours 2 3

3.

4.

4.5

5.

6.

7.

8. 9.

3 5

10.

4.5 Total Lectures: 39h Tutorials: 11h 50

Total hours

REFERENCE BOOKS: Fred Ramsey and Dan Schafer (2001) The Statistical Sleuth (2nd Edition). Duxbury Press. Julie Pallant (2007) SPSS Survival Manual: A step-by-step guide to data analysis using SPSS for Windows (3rd Edition). Open University Press. ASSESSMENT: 40% CA, 60% Exam. MODULE CO-ORDINATOR & LECTURER: A/Prof Teo Yik Ying (Tel: 6516 2760, E-mail: statyy@nus.edu.sg)
28

SEMESTER I & II LSM2101 METABOLISM AND REGULATION

Prerequisite: LSM1101 or LSM1401 Workload: 39 lecture hours + 11 tutorial hours Overview of the biosynthesis and catabolism of carbohydrates, proteins, lipids and nucleic acids in the context of human health and disease. Emphasis on the integration and regulation of metabolic pathways in different tissues and organs. Principles of bioenergetics and mitochondrial energy metabolism, free radicals, mitochondrial DNA damage in aging and neurodegenerative diseases will also be covered. S/N 1. Topics Introduction Overview of metabolism and general features in regulation of metabolic pathways. Bioenergetics ATPases, substrate-level phosphorylation, redox potential and free energy release Electron transport Oxidative phosphorylation Carbohydrate Metabolism Glycolysis and its regulation Metabolism of other hexoses HMP Glycogen: metabolism and regulation Gluconeogenesis and pentose phosphate pathway TCA cycle Lecture hours 1 Theresa Tan (semester 1) Sanjay Swarup (semester 2) 1 1 2 Theresa Tan (semester 1) Sanjay Swarup (semester 2) 2 1 1 2 1 2 Theresa Tan (semester 1) Sanjay Swarup & Ganesh Anand (semester 2) 2 1 1 1 1 2 Markus Wenk (semester 1) Adam Yuan (semester 2) 1 1 1 1 1 1 Yew Wen Shan (semester 1) Ganesh Anand (semester 2) 1 1 1 1 Theresa Tan (semester 1) Adam Yuan (semester 2)

2.

3.

4.

Lipid Metabolism Digestion, absorption and transport -oxidation of fatty acids Ketogenesis Fatty acid biosynthesis Synthesis of eicosanoids and membrane phospholipids Cholesterol metabolism

5.

Amino Acid Metabolism Overview of amino acid metabolism Transamination and deamination Urea synthesis and the urea cycle Metabolic fates of the carbon skeletons of amino acids Metabolism of selected amino acids Other specialised products derived from amino acid decarboxylation Regulation and integration of metabolism Enzyme and hormonal regulation of metabolic pathways Cellular compartmentation and organ specialization Fuel metabolism in the starve-fed cycle and during exercise Integration of metabolism of carbohydrates, lipids and proteins to ATP synthesis

6.

29

S/N 7.

Topics Nucleic Acid Metabolism Chemistry of nucleotides Synthesis of purine and pyrimidine ribonucleotides Formation of deoxyribonucleotides Nucleotide degradation

Lecture hours 1 2 1 1 Yew Wen Shan (Semester 1) Ganesh Anand (semester 2) 2 Theresa Tan (semester 1) Adam Yuan (semester 2) Total Lectures: 39h Tutorials: 11h Total hours 50h

8.

Free Radicals Reactive oxygen species formed through metabolic pathways Oxidative damage in ageing and neurodegenerative diseases Antioxidants

TEXT BOOK (Reference books): 1. Voet, D., Voet, J. and Pratt, C.: Principles of Biochemistry (3rd edition) MODULE CO-ORDINATORS: (Semester I) A/P Theresa Tan (Semester II) Dr Ganesh Anand LECTURERS: Semester I A/P Theresa Tan Dr Yew Wen Shan A/P Markus Wenk Semester II A/P Sanjay Swarup Dr Ganesh S Anand Dr Adam Yuan

(Tel: 6516-3685, E-mail: bchtant@nus.edu.sg) (Tel: 6516-7722; E-mail: dbsgsa@nus.edu.sg)

(Tel: 6516-3685, E-mail: bchtant@nus.edu.sg) (Tel: 6516-8624, E-mail: bchyws@nus.edu.sg) (Tel: 6516-3624, E-mail: bchmrw@nus.edu.sg)

(Tel: 6516-7933; E-mail: sanjay@nus.edu.sg) (Tel: 6516-7722; E-mail: dbsgsa@nus.edu.sg) (Tel: 6516-7202; E-mail: dbsyya@nus.edu.sg)

30

SEMESTER I & II LSM2102 MOLECULAR BIOLOGY

Prerequisite: LSM1101 (or LSM1401) and LSM1102 Workload: 39 lecture hours + 6 tutorial hours + 5 CA and Discussion This module deals with the structure, organization and function of genes and genomes in both prokaryotes and eukaryotes (eg: DNA topology, hierarchy of packaging of DNA in chromosomes and relationship to gene activity and genome dynamics). The functional roles of DNA regulatory cis-elements and transcription factors involved in gene expression will be examined extensively. The molecular events of transcription; post-transcriptional modifications and RNA processing; temporal and spatial gene expression, control and regulation, signals of gene expression will be dealt with in detail. The cause and/or effect of dysfunction of gene expression and diseases will be discussed. S/N 1. Topics Genes & Genome Dynamics o Introduction - Landmark discoveries & current trends in molecular biology o Gene density; complexity and genome manipulation o Chromosomes - DNA topology, packaging & hierarchy of the eukaryotic genome - Nucleosomes; solenoids; loops; scaffolds; - Telomeres and centromeres - Satellite DNA; repetitive DNA; gene families o Organelle genomes (mitochondrial and chloroplast genomes) Gene Expression and Regulation Gene transfer & genetic recombination: homologous, site-specific & transpositional recombination. DNA rearrangements Mutation and DNA Repair Epigenetics: Gene inactivation, Imprinting and Dosage compensation Gene expression in prokaryotes: o Prokaryotic RNA polymerase and transcriptional regulation; Regulatory elements o Prokaryotic operons & regulatory circuits; o Positive & negative control systems; catabolic response, attenuation, antitermination o Mutations to identify functionalities of operator, regulator & repressor Gene Expression in Eukaryotes Gene expression in eukaryotes: o Promoters; cis-elements (enhancers, silencers, LCRs) in eukaryotes o Eukaryotic RNA polymerases; transcription pre-initiation complex o Transcription factors (Zn fingers; coactivators; repressors) o Chromatin remodelling, Histone modification o Post-transcriptional processing: 5capping; polyadenylation; splicing, mis-splicing and diseases o Differential gene expression (spatial and temporal); o RNA interference (RNAi) gene silencing in control of expression o Translational control Lecturers Sem I Sem II

THP 13

LBC 13

2.

NL 13

CKL 13

3.

DJL 13

AY 13

Total Lectures: 39h Tutorials: 6h CA and Discussion: 5h Total hours 50h

MODE OF ASSESSMENT: CA (short questions & MCQs) = 40%; Final semestral exam (MCQs) = 60% MODULE COORDINATOR: Prof Ding Jeak Ling LECTURERS :( Semester I) (Semester II) A/P Too Heng Phon (THP)
Dr Norbert Lehming (NL) Prof Ding Jeak Ling (DJL) A/P Low Boon Chuan (LBC) A/P Chua Kim Lee (CKL) Dr Adam Yuan Yu-Ren (AY) (Tel: 6516-3687, E-mail: bchtoohp@nus.edu.sg) (Tel: 6516-3499, E-mail: micnl@nus.edu.sg) (Tel: 6516-2776, E-mail: dbsdjl@nus.edu.sg) (Tel: 6516-7834, E-mail: dbslowbc@nus.edu.sg) (Tel: 6516-3684, E-mail: bchckl@nus.edu.sg) (Tel: 6516-7202, E-mail: dbsyya@nus.edu.sg)

31

SEMESTER I & II LSM2103 CELL BIOLOGY

Prerequisite: LSM1101 (or LSM1401) and LSM1102 Workload: 39 lecture hours + 11 tutorial hours This course provides a comprehensive understanding of cellular structures, functions and interactions in unicellular and multi-cellular systems. Emphasis is on cellular functions. Topics include structure and functions of organelles, the cytoskeleton and cell movements, current concepts of intercellular and intracellular signaling, intracellular protein trafficking and organelle biogenesis (including endocytosis, exocytosis, import of proteins into organelles, nuclear transport), molecular basis of cell proliferation and apoptosis. S/N 1. 2 3 4 5 6 7 8 Introduction to LSM2103 Organelles Cytoskeleton (Actin microfilaments, microtubules & intermediate filaments) Organelle biogenesis Intracellular protein trafficking (including endocytosis and exocytosis) Intracellular cell signaling Apoptosis Cell proliferation Total lecture hours: Total tutorial hours: Total hours:
TEXT BOOKS:

Topics

Lecture hours 1 4 6 5 5 7 3 8 39h 11h 50h

Molecular Cell Biology 6th Edition, 2008 (Lodish & co-authors) Molecular Biology of the Cell, 5th Edition, 2009, Alberts & co-authors
A/P Chang Chan Fong Office phone: 6516-3681 E-mail: bchccf@nus.edu.sg E-mail: bchyfm@nus.edu.sg

MODULE CO-ORDINATOR: Semester 1

Semester 2 MODE OF ASSESSMENT:

Dr Yeong Foong May

Semester 1: CA1 (20%), CA2 (20%), Final examination (60%) Semester 2: CA1 (25%), CA2 (25%), Final examination (50%)

LECTURERS: A/P Chang Chan Fong Dr Chen Ee Sin Dr Yeong Foong May Dr Thilo Hagen

(Semester 1 & 2) (Semester 1) (Semester 2) (Semester 1 & 2)

(Tel: 6516-3681, E-mail: bchccf@nus.edu.sg) (Tel: 6516-5616, E-mail: bchces@nus.edu.sg) (Tel: 6516-8866, E-mail: bchyfm@nus.edu.sg) (Tel: 6516-3686, E-mail: bchth@nus.edu.sg)

32

SEMESTER I & II LSM2201A EXPERIMENTAL BIOCHEMISTRY

Prerequisite: LSM1101 or LSM1401 Workload: 12 lecture hours + 8 tutorial hours + 30 laboratory hours Emphasis on principles and understanding of methods of protein purification and characterization. The module integrates factual knowledge in various chromatographic methods to laboratory practice. Analysis and presentation of data from mini-project and from journal articles. S/N 1. Topics Introduction Initial planning, Sources, Extraction, Solubilization, Buffer selection, Additives in buffer, Clarification and concentration of extract, Saltingin and salting out, Ammonium sulfate precipitation, Solvent precipitation and role of temperature Column chromatography Introduction and concept; principles and applications of gel filtration, ion exchange chromatography, hydrophobic interaction chromatography, reverse phase chromatography, Affinity chromatography Dye ligand (Pseudo affinity) chromatography, immunoaffinity chromatography, Purification table, Optimization of purification strategy, scaling up and scaling down, measurement of protein and enzymatic activity Principles and applications of Electrophoresis (native and SDS PAGE, isoelectrofocusing, 2D electrophoresis), Capillary electrophoresis; mass spectrometry (Electrospray ionization, matrix assisted laser desorption time of flight) Lecture hours 2

2.

3.

Total Lectures: 12h Tutorials: 8h Practicals: 6x5= 30h Total hours: 50h

TEXT BOOKS: Protein Purification: Principles, High Resolution, Methods and Applications, J. C. Janson and L. Ryden; WileyLiss, New York, 1998 Protein Purification Techniques, S. Roe; Oxford University Press, Oxford, 2001 (2nd Edition) REFERENCE BOOKS: Protein Purification Methods: A Practical Approach, E. L. V. Harris and S. Angal; IRL Press, Oxford, 1995 Protein Purification: Principles and Practice, R. K. Scopes; Springer-Verlag, New York, 1982 MODE OF ASSESSMENT: 100% CA MODULE CO-ORDINATOR: Prof R. Manjunatha Kini (Semester I) A/P Maxey Chung (Semester II)

(Tel: 6516-5235, E-mail: dbskinim@nus.edu.sg) (Tel: 6516-3252, E-mail: bchcm@nus.edu.sg)

LECTURERS FOR PRACTICALS: Biochemistry A/P Maxey Chung (Tel: 6516-3252, E-mail: bchcm@nus.edu.sg) Lecture A/P Chang Chan Fong (Tel: 6516-3681, E-mail: bchccf@nus.edu.sg) Practical Dr Deng Lih Wen (Tel: 6516-1239, E-mail: bchdlw@nus.edu.sg) Practical Biological Sciences Prof RM Kini A/P Henry Mok A/P Jayaraman Sivaraman

(Tel: 6516-5235, E-mail: dbskinim@nus.edu.sg) (Tel: 6516-2967, E-mail: dbsmokh@nus.edu.sg) (Tel: 6516-1163, E-mail: dbsjayar@nus.edu.sg)
33

SEMESTER I & II LSM2202A EXPERIMENTAL MOLECULAR AND CELL BIOLOGY

Prerequisite: LSM1101 and/or LSM1102 Workload: 12 lecture hours + 30 laboratory hours + 8 tutorial hours This module introduces students to the theory and practical applications of techniques relevant to molecular and cell biology. The module emphasizes problem-solving exercises in the application of commonly used recombinant DNA techniques, including RNA isolation and characterization, reverse transcription, polymerase chain reaction (PCR), construction of recombinant DNA molecules, gel electrophoresis of RNA and DNA, DNA sequencing and analysis, and real-time PCR. The objective for the students is to learn HOW to perform the experiments, and to understand WHY these experiments have to be carried out in a certain way. Lecture Topics Overview of the module, outlining how the practical experiments demonstrate the generation of a cDNA clone from RNA RNA isolation and mRNA expression Molecular cloning I Molecular Cloning II Practical Topics

RNA isolation and characterization by nano-drop & agarose gel electorphoresis Reverse transcription and polymerase chain reaction (RT-PCR) Construction of recombinant DNA molecules I: DNA ligation and transformation Construction of recombinant DNA molecules II: DNA isolation and characterization by restriction digestion & agarose gel electrophoresis

DNA sequencing and analysis

DNA sequencing: Sequencing reactions, DNA sequencer and sequence data retrieval/analysis Real-Time PCR: SYBR-green & TaqMan assay and data analysis 12h 30h 8h 50h

Real-Time PCR and other relevant methodologies Lectures: Practicals: Tutorials: Total hours:

Reference Manuals: Current Protocols in Molecular Biology by Fred M. Ausubel, Roger Brent, Robert E. Kingston, David D. Moore, J.G. Seidman, John A. Smith, and Kevin Struhl. John Wiley & Sons, Inc. Module Coordinators: Prof K Jeyaseelan, Biochemistry A/P Wang Shu, Biological Sciences Dr Norbert Lehming, Microbiology

(Tel: 6516-3248, E-mail: bchjeya@nus.edu.sg) (Tel: 6516-7712, E-mail: dbsws@nus.edu.sg) (Tel: 6516-3499, E-mail: micln@nus.edu.sg )

Module Assessment: Lab reports: 20%; Midterm CA: 30%; Final CA: 50%

34

SEMESTER I & II LSM2203 EXPERIMENTAL MICROBIOLOGY

Prerequisite: LSM1101 or LSM1102 or LSM1103 Workload: 13 lecture hours + 32 laboratory hours This module gives an overview of microbial diversity, the biological properties of microbes, methods and approaches in the study of microbiology. At the end of the module, students should have fundamental knowledge of microbiology, including tools in the study of cells and microbes and the awareness of biosafety, and students should be excited by the microbial world and wishing to know more. S/N 1. 2. 3. 4. 5. 6. 7. 8. 9. Topics Introduction to the diversity of microbial world and phylogeny Biosafety Isolation and identification of microbes Microbes in the environment: Where are microbes found and why are they there Microbes and immunity Soil microbiology: Isolation, identification and characterization (antibiotic producers, polysaccharide producers) Water-borne pathogens: Isolation, enumeration, physiology and behaviour outside the host Food microbiology: Isolation, enumeration and characterization (yeast, lactic acid bacteria, enteric bacteria) Human skin microbiology: Isolation, are they pathogens? Lectures: 13h Practicals: 32h Total hours: 45h MODULE COORDINATORS: (Semester I) A/Prof Lee Yuan Kun/Prof Ng Mah Lee (Semester II) Dr Chu Jang Hann, Justin/A/P Sim Siow Suan Lecture hours 2h 1h 4h 4h 2h 8h 8h 8h 8h Practicals

(Tel: 6516-3284, E-mail: micleeyk@nus.edu.sg)/ (Tel: 6516-3283, E-mail: micngml@nus.edu.sg) (Tel: 6516-3278, E-mail: miccjh@nus.edu.sg)/ (Tel: 6516-3280, E-mail: micsimts@nus.edu.sg)

MODE OF ASSESSMENT: Continuous Assessment (90%) Laboratories (10%) LECTURERS Semester I: Assoc Prof Lee Yuan Kun Professor Ng Mah Lee Assist Prof Kevin Tan Semester II: Dr Chu Hang Hann, Justin Assoc Prof Sim Tiow Suan Assoc Prof Ho Bow

35

SEMESTER I & II LSM2241 INTRODUCTORY BIOINFORMATICS

Prerequisite: LSM1101 or LSM1401 Workload: 22 lecture hours + 40 laboratory hours with Presentation (CA1) and MCQ Midterm (CA2) Introduction to biological databases and bioinformatics software. Sequence comparison algorithms and tools. Biomolecular 3D structure and modelling. Students will be introduced to bioinformatics theory, tools, and techniques. Practical Bioinformatics cover database searching, sequence analysis via EMBOSS, BLAST and BLAST database building, Multiple Sequence Alignment with CLUSTALW and JalView, Molecular Phylogenetic Inference with MEGA, PyMol structural visualization and Homology Modelling and Structure Prediction with MODELLER and SWISSMODEL. Students will achieve highly valued skills as biological researchers with basic competence in computational and informatics techniques, with an option to learn more advance skills in LSM3241 Bioinformatics and Biocomputing. S/N 1. 2. Topics Lecture hours 6

3. 4.

5.

Introduction, Biological Databases and Bioinformatics Software Why Bioinformatics? A survey of biological databases. Bioinformatics tools and techniques Biological Sequence Comparison, Alignments, Patterns, and Motifs: Theory, Tools and 6 Algorithms Principles of sequence alignment. Fundamentals of sequence comparisons. Basics of BLAST database searches. Biological patterns and profiles. Molecular Phylogenetics 4 Principles of molecular evolution. Techniques in phylogenetic inference Principles of Structural Biology, Biomolecular visualization and Structure Prediction 4 Basics of structural biology and structural databases. Modelling bio-molecular structure from sequence. Latest Developments in Bioinformatics 2 Personal Genomics and the future of bioinformatics Total Lectures: 11 x 2 = 22 h Practicals / Tutorials: 4 x 10 = 40 h Total hours: 62h

TEXT BOOK (Reference books):

Bioinformatics and Functional Genomics. Jonathan Pevsner. 2009. Wiley-Blackwell; ISBN: 0470085851
MODE OF ASSESSMENT: CA1 : 20% (10% Presentation + 5% Peer Review + 5% practical attendance) CA2 : 30% (MCQ) Final Exam : 50% (MCQ + Problem-Based Essays) MODULE CO-ORDINATORS: A/P Tan Tin Wee A/Prof Christopher Hogue LECTURERS: A/Prof Tan Tin Wee A/Prof Christopher Hogue Visiting Prof Patrice Koehl Visiting Prof Greg Tucker-Kellogg Adjunct Prof Victor JC Tong

(Tel: 6516-3566, E-mail: tinwee@bic.nus.edu.sg) (Tel: 6516-7066, E-mail: dbshcwv@nus.edu.sg)

(Tel: 6516-3566, 96640347, E-mail: tinwee@bic.nus.edu.sg) (Tel: 6516-7066, 94875537, E-mail: dbshcwv@nus.edu.sg) (UC Davis, E-mail: koehl@cs.ucdavis.edu) (E-mail: dbsgtk@nus.edu.sg) (I2R, E-mail: victor@bic.nus.edu.sg)

36

SEMESTER I & II LSM2251 ECOLOGY AND ENVIRONMENT

Prerequisite: LSM1103 Workload: 26 lecture hours + 6 tutorial hours + 18 practical hours including one Saturday fieldtrip This module introduces students to the science of ecology and its role in understanding environmental processes. It covers both the major concepts and their real-world applications. Topics will include models in ecology, organisms in their environment, evolution and extinction, life history strategies, population biology, ecological interactions, community ecology, ecological energetics, nutrient cycling, landscape ecology. S/N 1. 2. 3. 4. 5. 6. 7. 8. Topics Course overview. What is Ecology? Why is it relevant? The physical environment; aquatic vs terrestrial environments; physical variables; endotherms and ectotherms; water relations in plants and animals. Genetics and ecology; evolution and extinction. Population ecology; distributions and abundance; population dynamics; population growth models and reality; life history strategies. Ecological interactions: intra- and inter-specific competition; niches; predation; herbivory; parasitism and disease; mutualisms. Community ecology; assemblages or superorganisms; types of communities; community structure; diversity and stability; succession. The ecosystem concept; ecological energetics; primary production; energy flow; trophic levels; energy and carbon; nutrient cycling. Landscape ecology; landscape structure and processes; landscape change; global ecology; the Gaia hypothesis; human impacts on global processes. Practicals/Tutorials: Total hours: TEXT BOOK: Molles, M. C., 2009. Ecology: Concepts and Applications. McGraw Hill, 5th Edition. Smith, T. M. & R. L. Smith, 2009. Elements of Ecology. Pearson/Benjamin Cummings, 7th Edition. MODE OF ASSESSMENT: 50% - Continual Assessments (Tests and reports) 50% - Final Exam (Closed Book; short answers and essays) MODULE CO-ORDINATOR: Mr N Sivasothi LECTURERS: Prof Richard Corlett Mr N Sivasothi Lecture hours (26 hours) 2 4 2 4 4 4 2 4 6 x 4h= 24h 50h

(Tel: 6516-8869; E-mail: sivasothi@nus.edu.sg)

(Tel: 6516-1285; E-mail: Corlett@nus.edu.sg) (Tel: 6516-8869; E-mail: sivasothi@nus.edu.sg)

37

SEMESTER I & II LSM3211 FUNDAMENTAL PHARMACOLOGY

Prerequisite: LSM2101 or LSM2103 Preclusion: GEK2501 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This module aims to provide basic principles of pharmacological science with emphasis on drug action from the molecular and cellular levels to tissue, organ and whole animal including humans using lectures and practicals. The lectures will start with the classical drug receptor theory followed by molecular pharmacology of drug receptors and their regulation including receptor-mediated signal transduction and membrane ion channel function. Autonomic pharmacology (sympathetic and parasympathetic) will be introduced. The module also focuses on the pharmacodynamics of autacoids, non-steroidal anti-inflammatory agents, corticosteroids and other immunosuppressants, anti-asthma drugs, antithrombotic agents and nitric oxide. S/N Topics Lecture hours Semester Semester I II 1 FW 1 FW 2 GS 5 FW 4 PW 2 GS 5 FW 4 PW

1 2 3

Introduction to Pharmacology Fundamental Pharmacokinetics Mechanism of Drug Action: Principles of drug actions Structure-Activity Relationship G Protein-Coupled Receptor Intracellular Receptor Transmembrane Receptor Enzyme Cytokine receptor Ligand-gated Ion Channels Voltage-gated Ion Channels Receptor Regulation Receptor Classification Autonomic Pharmacology: Adrenergic & Cholinergic Inflammation Pharmacology: Autacoids Eicosanoids Vasoactive Peptides Corticosteroids Immunosuppressants Anti-asthma Drugs Non-steroidal Anti-inflammatory Drugs Anti-Rheumatoid Arthritis Drugs Anti-Coagulation Drugs Anti-Ulcer Drugs

4 5

4 PKM 4 PW 6 FW

4 PKM 4 PW 6 FW

Total Lectures : 26h Tutorials: 6h Practicals: 3X6= 18h Total hours: 50h

TEXTBOOK: Pharmacology by HP Rang, MM Dale, JM Ritter & RJ Flower, Churchill Linvingstone, 6th Edition, 2007 MODE OF ASSESSMENT: MCQ/Long Essay (30% CA, 70% Final Examination) MODULE CO-ORDINATOR: A/P Fred Wong Wai Shiu (FW) LECTURERS: Professor Peter Wong Tsun Hon (PW) Professor Philip K Moore (PKM) Dr Gautam Sethi (GS)

(Tel: 6516-3263; E-mail: phcwongf@nus.edu.sg)

(Tel: 6516-3224, E-mail: phchead@nus.edu.sg) (Tel: 6601-1669, E-mail: dprmpk@nus.edu.sg) (Tel: 6516-3267, E-mail: phcgs@nus.edu.sg)
38


SEMESTER I & II LSM3212 HUMAN PHYSIOLOGY: CARDIOPULMONARY SYSTEM
Prerequisite: LSM2101 Workload: 24 lecture hours + 6 tutorial hours + 10 practical hours + 10 hours assignments/Self directed study The heart and lungs are central to the maintenance of homeostasis in the human body by bringing essential materials to and removing wastes from the bodys cells. This module covers the basic physiology of the cardiovascular and pulmonary systems using exercise to illustrate the onset of homeostatic imbalances and the bodys responses to restore homeostasis. Students will be able to identify the benefits that exercise imparts to cardiorespiratory fitness and overall health. S/N 1 2 Topics Introduction to exercise physiology Blood Overview and Composition and functions of blood Blood cell production and Hemostasis Blood Grouping 3 Cardiovascular System Autonomic nervous system Electrical activity of the heart Cardiac mechanics, cardiac cycle Cardiac output Blood pressure Relevance of exercise to the CVS 4 Respiratory System Breathing Gas Exchange, Gas Transport Control of Breathing Relevance of exercise to the RS Practicals Effects of exercise on ECG and heart function Metabolic (VO2 max) and postural hypotension Postural hypotension Lecture hours Semester I Semester II FL (guest) 6 WCT FL (guest) 6 HS

6 LL

6 LL

2- FL (guest) 6 DY

2-FL (guest) 6 BL

2- FL (guest)

2- FL (guest)

1 2 3

Total lecture hours : 24h Total tutorial hours : 6h Total practical hours : 10h Assignments/Self directed study : 10h Total hours : 50h TEXT BOOK: L. Sherwood 2004: Human Physiology: From cells to Systems 5th edition MODE OF ASSESSMENT: 60% Continual Assessments (20% midterm test; 40% practical reports + assignments), 40% Final Exam MODULE CO-ORDINATOR: Dr Lina HK Lim (LL) LECTURERS: Semester I Dr Lina HK Lim (LL) Dr Deng Yuru (DY) A/P Wong Chong Thim (WCT) Dr Fabian Lim (DSO) (FL) Semester II Dr Lina HK Lim (LL) Dr Bernard Leung (BL) A/P Herbert Schwarz (HS) Dr Fabian Lim (DSO) (FL)

(Tel: 6516-5515, E-mail: phslhkl@nus.edu.sg)

(Tel: 6516-5515, E-mail: phslhkl@nus.edu.sg) (Tel: 6516-1935, E-mail: phsdy@nus.edu.sg) (Tel: 6516-3232, E-mail: phswct@nus.edu.sg) (E-mail: limcl@dso.org.sg) (Tel: 6516-5515, E-mail: phslhkl@nus.edu.sg) (Tel: 6516-4227, E-mail: phslplb@nus.edu.sg) (Tel: 6516-7773, E-mail: phssh@nus.edu.sg) (E-mail: limcl@dso.org.sg)
39

SEMESTER II LSM3213 MOLECULAR & CELLULAR NEUROBIOLOGY

Prerequisite: LSM2102 and LSM2103 Workload: 26 lecture hours + 12 tutorial hours + 12 practical/SDL hours This module aims firstly to introduce third year students to neurobiology and secondly to impart fundamental concepts and principles in neurophysiology, neuropharmacology, neurochemistry and cell biology of the nervous system. The fascination with how the brain works remains to this day and it is still very much a mystery. The first step on this quest begins with understanding the fundamental principles underlying the molecular and cellular functions of neuronal and glial cells, the building blocks of the brain. This module seeks (1) to inform; (2) to challenge on the basis that scientific enquiry is uncertain while knowledge is tentative and (3) to relate neurobiology to our lives. The topics include: (1) Neuronal signalling; (2) Mechanisms of neurotransmission; (3) Neuronal growth, regeneration and degeneration and (4) Glial function and neurogrowth factors. S/N Lecture Topics hours Neuronal Signalling & Neurophysiology 1. Brief introduction on functional anatomy of the brain Ionic basis of generation and maintenance of resting membrane potential and action potential 6 Molecular biology of voltage-gated ion channels involved in electrical signalling Mechanism of neuromuscular excitation-contraction coupling Channelopathies: ion channel disorders Mechanisms of neurotransmission & neuropharmacology 2. Role of neurotransmitters in synaptic signalling Mechanisms of neurotransmitter release 6 Molecular biology of neurotransmitter receptors involved in reception of chemical signals Formation of synapse at the neuromuscular junction and central synapse Integration of synaptic signals Neuronal growth, regeneration and degeneration 3. Neuronal polarity: membrane dynamics and trafficking in neurons 6 Neuronal growth and developmental pathfinding; neuronal regeneration and inhibitors of regeneration Neuronal death and neurodegeneration Glial functions and neurogrowth factors 4. Glial cell and molecular biology 6 Cellular and molecular biology of neurogrowth factors (NGF/GDNF) and receptors Molecular and cellular aspects of Parkinsons disease 5. Protein aggregation and neurodegeneration 2 Total Lectures: 26h Tutorials/CA: 12h Practicals/SDL: 12h Total hours: 50h

TEXT BOOK: 1. Neuroscience: Exploring the Brain - Mark F. Bear, Barry W. Connors and Michael A. Paradiso. 2. Essentials of Neural Science and Behavior - Eric R. Kandel, James H. Schwartz and Thomas M. Jessell 3. The Neuron: Cell and Molecular Biology - Irwin B. Levitan and Loenard K. Kaczmarek MODE OF ASSESSMENT: 20% CA, 20% SDL and .60% Final Exam MODULE CO-ORDINATOR: A/P Soong Tuck Wah LECTURERS: Dr Low Chian Ming A/P Soong Tuck Wah A/P Tang Bor Luen A/P Too Heng Phon

(Tel: 6516-1938; E-mail: phsstw@nus.edu.sg)

(Tel: 6516-5841; E-mail: phclowcm@nus.edu.sg) (Tel: 6516-1938; E-mail: phsstw@nus.edu.sg) (Tel: 6516-1040; E-mail: bchtbl@nus.edu.sg) (Tel: 6516-3687; E-mail: bchtoohp@nus.edu.sg)
40

SEMESTER II LSM3214 HUMAN PHYSIOLOGY: HORMONES AND HEALTH

Prerequisite: LSM2101 and LSM3212. No co-reading of LSM3212 and LSM3214 Workload: 26 lecture hours + 14 tutorial hours + 10 hours assignment and 3 CAs This module covers several human physiological systems using hormonal control of homeostasis as a basis for understanding normal function and health. The student will be able to appreciate the interactions occurring amongst the endocrine, digestive, renal, and reproductive systems, and be able to relate them to the body's biological rhythms (or clocks), growth, responses to stress, and reproductive processes. Major Topics Covered: endocrine system, central endocrine glands, peripheral endocrine glands, digestive system, digestive processes, energy balance, urinary system, fluid processing, fluid balance, reproductive system, male reproductive physiology and female reproductive physiology. S/N 1. Topics Endocrine System Overview of the central endocrine glands pineal gland, hypothalamus, posterior pituitary gland, and anterior pituitary gland Overview of the peripheral endocrine glands thyroid gland and adrenal glands Calcium metabolism Reproductive System Male reproductive physiology Female reproductive physiology Pregnancy Digestive System Digestive processes gastrointestinal tract, digestion and absorption, and gastrointestinal hormones Energy balance metabolism and endocrine control of growth Appetite control Renal System Fluid processing anatomical structures, glomerular filtration, hormonal regulation, and urine formation and excretion Fluid balance body fluid compartments, hormonal control of volume and osmotic balance Lecture hours 6

2.

3.

8 LCW

4.

8 WCT

Total Lectures: 26h Tutorials: 14h Others: 10h Total hours: TEXT BOOK: Sherwoods Human Physiology From Cells to Systems, 7th Edition 2010 MODE OF ASSESSMENT: 60% CAs and assignment, 40% Final Exam MODULE CO-ORDINATOR: A/P Wong Chong Thim (WCT) LECTURERS: A/P Wong Chong Thim (WCT) A/P Lee Chee Wee (LCW) (Tel: 6516-3232; E-mail: phswct@nus.edu.sg) (E-mail: phsleecw@nus.edu.sg) 50h

(Tel: 6516-3232; E-mail: phswct@nus.edu.sg)

41

SEMESTER II LSM3221 HUMAN PHARMACOLOGY

Prerequisite: LSM3211 Workload: 26 lecture hours + 6 tutorial hours + 18 practical hours This module introduces the pharmacological treatment of human disease. We will look at actions of drugs on major organ systems in the human body. Examples of drugs used to treat diseases and disorders of the cardiovascular, endocrine and nervous systems and chemotherapeutic agents will be discussed. S/N 1 Topics Drugs Affecting the Cardiovascular System Vasodilators Diuretics Lipid Lowering Drugs Drugs Affecting the Endocrine Systems Pancreatic Hormones: Glucagon and Insulin Oral Antidiabetic Drugs Thyroid Hormones and Disorders Sex Hormones and Contraceptives Drugs Affecting the Nervous System General Anaesthetics Local Anaesthetics Antidepressant Drugs Antipsychotic Drugs Drugs Used in Parkinsons Disease Drugs Used in Alzheimers Disease Chemotherapeutic Agents Principles of Chemotherapy for Cancer Conventional Anti-Cancer Drugs Antivirals Antibiotics Adverse Drug Reaction and Drug Interactions Adverse Drug Reactions Drug Interactions Lecture hours 6 BJ

5 BJ (Guest lecturer: SMK)

9 GD

4 GS (Guest lecturer: BT) 2 (Guest lecturer: TCH) Lectures: 26h Tutorials: 6h Practicals: 18h Total hours 50h

TEXTBOOK:

Pharmacology by H.P. Rang, M.M. Dale, J.M. Ritter, R.J. Flower and G. Henderson

MODE OF ASSESSMENT: MCQs/Short Essay/Short-Answer Questions (50% CAs, 50% Final Exam) MODULE CO-ORDINATOR: A/Prof Dawe, Gavin Stewart (GD) LECTURERS: A/Prof Bian Jinsong (BJ) Dr Gautam Sethi (GS) GUEST LECTURERS: A/Prof Benny Tan (BT) A/Prof Sim Meng Kwoon (SMK) A/Prof Tan Chay Hoon (TCH)

(Tel: 6516-8864; E-mail: phcdgs@nus.edu.sg)

(Tel: 6516-5502; E-mail: phcbjs@nus.edu.sg) (Tel: 6516-3267: E-mail: phcgs@nus.edu.sg)

(Tel: 6516-3267: E-mail: phcgs@nus.edu.sg) (Tel: 6516-3268: E-mail: phcsimmk@nus.edu.sg) (Tel: 6516-3310: E-mail: phctch@nus.edu.sg)

42

SEMESTER I & II LSM3223 IMMUNOLOGY

Prerequisite: LSM2103 Workload: 24 lecture hours + 24 laboratory hours + 2 tutorial hours This course provides the central concepts of immunology and the foundation for understanding how immunity functions. The subjects of innate immunity and haematopoiesis introduce the origin and role of different cell types in immunity. The mechanics of how the body protects itself from disease are explored in relation to T and B cell biology, antibody-antigen interaction, major histocompatibility complex and antigen presentation. Other topics include hypersensitivity, immunodeficiencies, immune tolerance, autoimmunity, resistance and immunization to infectious diseases. S/N Topics Introduction to immunology - Overview of the immune system - Cells and structures of the immune system - Antigens - Innate immunity Humoral immunity and effector mechanisms - Immunoglobulin structure and function - Antigen-antibody interactions Antigen recognition and immune interactions - B cell development and activation - Complement - Generation of immunoglobulin and T cell receptor diversity - Major Histocompatibility Complex - Antigen processing and presentation Cellular immunology and immune regulation - Cell-mediated immunity - Cytokines and chemokines - T cell development and activation - Adaptive immunity Infection immunity - Viruses and parasites - Bacteria and fungi Immunity in disease - Hypersensitivity - Immunological tolerance - Autoimmunity - Tumour immunology - Immune evasion Research applications - Vaccines and immunization - Module summary/discussion Lecture hours Semester I Lecture hours Semester II

1.

2.

3.

4.

5.

6.

7.

TEXTBOOKS: 1. Roitts Essential Immunology (Blackwell) Roitt and Delves. 2. Immunobiology: The Immune System in Health and Disease (Garland) Janeway. 3. Immunology (Mosby) Roitt et al. 4. Cellular and Molecular Immunology (Saunders) Abbas et al. MODE OF ASSESSMENT: 30% - Continuous Assessment (MCQ) 70% - Final Written Examination

43

MODULE CO-ORDINATORS: Dr Wong Siew Heng Dr Paul Macary LECTURERS: Semester I Dr Wong Siew Heng A/Prof Chew Fook Tim A/Prof Gan Yunn Hwen Semester II Dr. Paul Macary Prof Kemeny D Michael A/Prof. Lu Jinhua

(Semester I) (Semester II)

(Tel: 6516-3517, E-mail: micwsh@nus.edu.sg) (Tel: 6516-5482, E-mail: micpam@nus.edu.sg)

(Tel: 6516-3517, E-mail: micwsh@nus.edu.sg ) (Tel: 6516-1685, E-mail: dbscft@nus.edu.sg ) (Tel: 6516-3678, E-mail: bchganyh@nus.edu.sg)

(Tel: 6516 5482, E-mail: micpam@nus.edu.sg) (Tel: 6516-3275, E-mail: mickdm@nus.edu.sg) (Tel: 6516-3277, E-mail: mickdm@nus.edu.sg)

44

SEMESTER II LSM3224 MOLECULAR BASIS OF HUMAN DISEASES

Prerequisite: Pass in LSM 2101 and LSM2103 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This module aims to provide students with in-depth knowledge of the basic mechanisms of common human diseases, such as cancer, atherosclerosis, obesity, diabetes, muscle wasting conditions and to prepare them for future translational research. This module focuses on the current molecular mechanisms underlying the pathogenesis of each disease. There will be extensive discussion on results from current cutting-edge research. Prospective students should have basic knowledge of molecular and cell biology, genetics and general human physiology before registering for this module. S/N Topics Lecture hours COURSE INTRODUCTION 1 Sharma M Mechanisms and Pathogenesis 2 hours Animal Models 2 CANCER Carcinogens & carcinogenesis Review of regulation of cell cycle & mitogenic signaling pathways Proto-oncogenes and oncogenes Tumour suppressors SKELETAL MUSCLE WASTING AND REGENERATION Muscle development and process of muscle regeneration Cancer Cachexia, Sarcopenia and Atrophy Muscular Dystrophy ATHEROSCLEROSIS Role of Inflammation Lipids and lipoprotein metabolism OBESITY Role of Leptin and other hormones Understanding the Fat equation DIABETES Glucose metabolism and Insulin signalling METABOLIC SYNDROME Role of genetic and environmental factors Yeong FM 8 hours

Sharma M 8 hours

Wong BS 2 hours Wong BS 2 hours Wong BS 2 hours Wong BS 2 hours Total Lectures : 26h Tutorials: 6h Practicals: 3 x 6 = 18h Total hours: 50h

6 7

TEXT BOOK (Reference books): Tomlinson, Heagerty & Weetman (1997) Mechanism of disease: an introduction to clinical science (Cambridge University Press). MODE OF ASSESSMENT: Laboratories: 20% Mid-term: 30% Final Examinations: 50% MODULE CO-ORDINATOR: A/P Mridula Sharma LECTURERS: A/P Mridula Sharma Dr Wong Boon Seng Dr Yeong Foong May

(There will be three 6-hour laboratory sessions)

(Tel: 6516 7102, E-mail: bchmridu@nus.edu.sg)

(Tel: 6516 7102, E-mail: bchmridu@nus.edu.sg) (Tel: 6516 7617, E-mail: phswbs@nus.edu.sg) (Tel: 6516 8866, E-mail: bchyfm@nus.edu.sg)
45

SEMESTER II LSM3225 MOLECULAR MICROBIOLOGY IN HUMAN DISEASES

Prerequisite: LSM2101 or LSM2102 or LSM2103 Workload: 24 lecture hours + 16 laboratory hours + 2 tutorial hours With the application of advanced technologies in molecular biology to the study of microorganisms, there are many implications on how we can identify and detect microbes, as well as treat and prevent diseases caused by both existing and newly emerged pathogens. In this course, the students will be taught the molecular principles of the physiological processes involved in the life cycle of different types of microbes and how these affect human health and disease. There are also practicals and specialized talks by guest lecturers to emphasis the application of molecular microbiology in laboratories that handle the diagnosis and surveillance of infectious diseases. S/N 1 Topics Introduction 1. Introduction to molecular microbiology 2. Evolution of microbes/Introduction to host-pathogen relationships 3. The infectious disease mechanism (requirement and transmission)/Control and treatment of microbial growth Viruses 1. Analysis of viral genomes - Basic principles 2. Relating viral sequence to structure and function 3. Viral evolution and pathogenesis 4. Identification of targets for the development of antiviral 5. Identification of virulence factors 6. Antivirals and antiviral resistance Bacteria and Fungi 1. Introduction to Bacteriology-Basic principles and diagnostic methods 2. Pathogenesis of bacterial diseases 3. Host immune responses to bacterial infection 4. Fungi and fungal infection Parasites 1. Introduction to medical parasitology 2. Diagnostic parasitology 3. Host-Parasite Interactions 4. Anti-parasite Strategies 5. Emerging parasitic pathogens Advanced techniques and translational microbiology 1. Advanced methods in microbiology 2. Opportunities to use pathogens and their components for therapy Lecture hours 4h

4h

4h

4h

4h

Infectious Diseases outbreak investigation and public health surveillance

2h

Epidemiology of infectious disease

2h

Practical Sessions 1. One-step Real-Time PCR detection and quantification of Chikungunya virus infection (4h) 2. Using web-based software for molecular microbiology analysis (2h) 3. Tutorial session for Using web-based software for molecular microbiology analysis (4h) 4. (a) Diagnostic Parasitology: Demonstrations of medically-important parasites (amoebas, ciliates, flagellates and apicomplexa); (b) Modern
46

approaches for detecting drug resistance in malaria: PCR and fluorescent drugs (4h) 5. Bacterial infection and host responses (4h)

Total Lectures Practicals/tutorial Total hours TEXTBOOK.

24h 18h 42

Strlkauskas, Strelkauskas and Moszyk-Strelkauskas, Microbiology, a clinical approach, Garland Science, Taylor & Francis Group, New York. 2010. RECOMMENDED COURSE SUPPORT MATERIAL http://www.tulane.edu/~wiser/protozoology/guide.html http://blast.ncbi.nlm.nih.gov/Blast.cgi http://mfold.rna.albany.edu/ http://jura.wi.mit.edu/bioc/siRNAext/register.php http://www.fludb.org/brc/home.do?decorator=influenza MODE OF ASSESSMENT: 40% - Continuous Assessment 60% - Final Examinations MODULE COORDINATOR: Justin Chu Tan Yee Joo Co-coordinator LECTURERS: Kevin Tan Volker Patzel Zhang Yongliang GUEST LECTURERS: Ooi Eng Eong Raymond Lin

(Tel: 6516-3278, E-mail: miccjh@nus.edu.sg) (Tel: 6516-3692, E-mail: mictyj@nus.edu.sg)

47

SEMESTER I LSM3231 PROTEIN STRCUTURE AND FUNCTION

Prerequisite: LSM2101 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours The main objective is to provide a strong foundation in the study of protein structure and function. The following topics will be covered: Structures and structural complexity of proteins and methods used to determine their primary, secondary and tertiary structures; Biological functions of proteins in terms of their regulatory, structural, protective and transport roles; The catalytic action of enzymes, their mechanism of action and regulation; Various approaches used in studying the structure-function relationship of proteins. S/N 1. Topics Introduction Protein structures Overview of protein structure Structural patterns in protein Varieties of protein structures Protein function Structural diversity reflects functional diversity in globular proteins Structure-function relationships in selected protein families Protein folding and molecular chaperones Advanced enzymology Enzymes, enzyme reaction kinetics, mechanism of action, and allosteric control of enzyme activity Probing structure function relationships Chemical modification Epitope mapping, Site-directed mutagenesis Methods for determination of protein structures Primary structure by Edman degradation and mass spectrometry Solid phase peptide synthesis and applications of synthetic peptides Secondary structure by circular dichroism and theoretical methods Tertiary structure by X-ray diffraction and NMR Prediction, engineering and design of protein structure Lecture Hours

Maxey Chung 8h

2.

Theresa Tan 8h

3.

Maxey Chung 4h Henry Mok 6h

Total Lectures: 26h Tutorials: 6h Practicals: 6x3= 18h Total hours: 50h

REFERRENCE BOOKS: Introduction to Protein Structure (2nd Edition) by Carl Branden and John Tooze; Introduction to Protein Architecture by Arthur M. Lesk , and Introduction to Protein Science by Arthur M. Lesk. MODE OF ASSESSMENT: CA, 40% (short answer questions); semestral examination, 60% (short answer and long answer questions) MODULE CO-ORDINATORS: A/P Maxey Chung

(Tel: 6516-3252, E-mail: bchcm@nus.edu.sg)

LECTURERS: A/P Maxey Chung, A/P Theresa Tan and A/P Henry Mok

48

SEMESTER I & II LSM3232 MICROBIOLOGY

Prerequisite: LSM2101 or LSM2102 or LSM2103 Workload: 26 lecture hours + 21 laboratory hours + 2 tutorials + 1 CA Principles of microbiology, with emphasis on the properties, functions and classification of the major classes of microorganisms, especially bacteria, fungi and viruses. Understanding microbial activities and their influence on microbial diseases, industrial applications, ecology, food and water quality. S/N 1 Topics Scope of microbiology: the diversity of the microbial world and microbial taxonomy Microbial structure and function: microbial physiology, microbial nutrition and microbial growth Food microbiology Environmental microbiology Medical microbiology: Microbial diseases and their control Practicals (7 x 3 hours) Basic Microscopy & Staining Physiological effects on microbial growth Microbial physiology Medical microbiology Food microbiology Environmental microbiology Water microbiology Lecture hours Semester I Semester II 2 2 STS STS 8 STS 8 HB 4 STS 4 KT 8 STS 8 HB 4 STS 4 KT

3 4 5

1 2 3 4 5 6 7

Total Lectures: 26 hours Total Practicals: 21 hours Tutorials: 2 hours CA: 1 hour Total hours: 50 TEXTBOOKS (Reference books): 1. Microbiology 6th Ed. (2004) Prescott, Harley & Klein, McGraw Hill. 2. Microbiology: A Human Perspective. (2004) EW Nester et al, McGraw Hill. 3. Biology of Microorganisms 11th Ed. (2006) Brock & Madigan, Prentice-Hall. 4. Microbiology: Principles and Explorations 6th Ed (2005) Black, John Wiley. 5. Topley and Wilsons Microbiology and Microbial Infections 9th Ed. (1998) Collier et al., 5 vols. MODE OF ASSESSMENT: 40% Continuous Assessment 60% Final Examination MODULE COORDINATOR: A/P Sim Tiow-Suan (STS) LECTURERS: A/P Sim Tiow-Suan (STS) A/P Ho Bow (HB) Dr Kevin Tan Shyong Wei (KT)

(Tel: 6516-3280, E-mail: micsimts@nus.edu.sg)

(Tel: 6516-3280, E-mail: micsimst@nus.edu.sg) (Tel: 6516 3672, E-mail: michob@nus.edu.sg) (Tel: 6516-6780, E-mail: mictank@nus.edu.sg)

49

SEMESTER I LSM3233 DEVELOPMENTAL BIOLOGY

Prerequisite: LSM2103 Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This course will showcase and examine embryogenesis, starting from fertilization to birth in the case of animal development; and to germination, growth and differentiation in plants. Students will be exposed to concepts, principles and mechanisms that underlie development in plants and animals. Different model organisms will be studied to demonstrate the rapid advances in this field of life sciences. S/N 1. 2. 3. 4. Topics Lecture hours 1 Loh CS 2 Loh CS 2 Loh CS 4 Loh CS

5.

6.

7.

8.

9.

10. 11. 12.

Plant Development : An Introduction Objectives, approach and what are we going to learn From Seeds to Plant Post-embryonic development. The organization and function of the apical meristem Leaf Positioning and Development Phyllotaxy and the determination of leaves Flowering and Flower Development Juvenility and phase change, competence and determination in flowering, physiological studies of flowering, light and flowering, genes that regulate flower development Pollen, Embryo Sac Development and Fertilization 3 Loh CS From diploid to haploid cells: development of the pollen and the ovule. The union of gametes in plant and embryo formation Animal Development: An Overview Historical perspective, philosophical approaches and milestones in the study of animal 2 embryology. Principles, concepts and tools in modern developmental biology Winkler C Fertilization: Starting a new organism 2 The union of gametes, cytoplasmic determinants, cleavage patterns, maternal effect Winkler C and zygotic control From Eggs to Embryos: Morphogenesis 2 Process and significance of gastrulation and neurulation, concepts of embryonic Winkler C induction and axis formation, determination and differentiation Pattern Formation I: Development of the nervous system 2 Cell migration and differentiation, morphogen gradients, positional information, Winkler C signalling cascades Pattern Formation II: Limb development 2 Formation and regeneration of limbs Winkler C Pattern Formation III: Segmentation 2 Somitogenesis in vertebrates; body segment formation in invertebrates Winkler C Model organisms and the determination of sex 2 C. elegans, Drosophila, zebrafish and mouse: evolution of sex determination Winkler C Total Lectures: 26h Tutorials: 6h Practicals: 6x3= 18h Total hours: 50h

TEXT BOOK (Reference books): Plant Development, the Cellular Basis by RF Lyndon, Publisher: Unwin (selected chapters) Developmental Biology of Flowering Plants by V Raghavan, Publisher: Springer-Verlag (selected chapters) Molecular Plant Development , from Gene to Plant by Peter Westhoff (selected chapters only) Plant growth and Development: a molecular approach by DE. Fosket, Academic Press (general reference) Developmental Biology by Scott F. Gilbert. Publisher: Sinauer (selected chapters) Essential Cell Biology by B. Alberts et al., Publisher: Garland Science (general reference) MODULE CO-ORDINATOR: A/P Christoph Winkler LECTURERS: A/P Christoph Winkler A/P Loh Chiang Shiong

(Tel: 6516-7376, E-mail: dbswcw@nus.edu.sg)

(Tel: 6516-7376, E-mail: dbswcw@nus.edu.sg) (Tel: 6516-2916, E-mail: dbslohcs@nus.edu.sg)

50

SEMESTER II LSM3241 BIOINFORMATICS & BIOCOMPUTING

Prerequisite: LSM2102 and either LSM2104 or LSM2241 Workload: 26 lecture hours + 30 tutorial hours/ laboratory hours This module provides intermediate to advanced topics in bioinformatics. Topics include sequence analysis and applications, basic programming for bioinformatics applications, molecular modeling and drug design, protein modeling, data mining and machine learning in biomedical sciences. Besides hands-on practical classes, students are involved in a project in which skills taught in the course will be applied to solve a biological research problem. Students of this module achieve proficiency in computational skills which are highly sought after in todays competitive biological research laboratories.

S/N

Topics Bioinformatics Resource Solving Biological Problems with Bioinformatics Software Implementation. Concepts in databases. Knowledge discovery: Ontologies and Data grammar (XML) Basic Bioinformatics Scripting Concepts in programming. Introduction to Algorithms in Bioinformatics. Machine Learning techniques in biological data analysis Machine learning I (SVM). Machine learning II (RF). Molecular Modeling and Rational Drug Discovery and Design Advanced Computational Structural Biology: Structural Modeling and Molecular Dynamics; Computational Drug Design Protein Interactions, Biological Pathways and Simulation Modelling of biological pathways; Analyzing Protein-Protein Interactions Development of Bioinformatics Discussion: Journal Paper Classic Practicals

Lecture hours 6

1.

2. 3.

4 4

4.

5.

6. 7.

2 30 Total Lectures: 26h Tutorials/ Laboratory: 30h Total hours: 56h

TEXT BOOK (Reference books): Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins Andreas D. Baxevanis, B. F. Francis Ouellette Edition: 3rd edition. Publisher: Wiley-Interscience

Introduction to Bioinformatics Arthur M. Lesk. 2008. Oxford University Press; ISBN: 9780199208043 Beginning Perl Simon Cozens & Peter Wainwright. 2000. Wrox Press; ISBN: 1861003145 Bioinformatics: The Machine Learning Approach (Adaptive Computation and Machine Pierre Baldi, Soren Brunak. 2001. The MIT Press; ISBN: 026202506X Molecular modelling : principles and applications. Andrew R. Leach. Imprint Harlow, England; Singapore: Pearson Education, c2001. ; ISBN: 0582382106
MODULE COORDINATORS: A/P Tan Tin Wee A/P Christopher Hogue LECTURERS: Prof Chen Yu Zong A/Prof Tan Tin Wee A/Prof Christopher Hogue Dr. Victor JC Tong Dr. Ng See Kiong Dr Xie Chao Professor Patrice Koehl (Tel: 6516-3566, E-mail: tinwee@bic.nus.edu.sg) (Tel: 6516-7066, E-mail: dbshcwv@nus.edu.sg)

Learning).

(Tel: 6516 6877, E-mail: csccyz@nus.edu.sg) (Tel: 6516-3566, 96640347, E-mail: tinwee@bic.nus.edu.sg) (Tel: 6516-7066, E-mail: dbshcwv@nus.edu.sg) (Guest Lecturer, I2R, E-mail: victor@bic.nus.edu.sg) (Guest Lecturer, I2R, E-mail : skng@i2r.a-star.edu.sg) (Guest Lecturer, LSI, E-mail: xiechao@bic.nus.edu.sg) (Visiting Professor, E-mail : koehl@cs.ucdavis.edu)

51

SEMESTER II LSM3242 APPLIED MICROBIOLOGY

Prerequisite: LSM3232 and either LSM2101 or LSM2102 or LSM2103. (LSM3232 can be read as co-requisite) Workload: 24 lecture hours + 8 tutorial hours/industrial visits + 18 laboratory hours This module covers the underlying scientific principles and the wide-ranging industrial, environmental, pharmaceutical, and biomedical applications of microbiology. The objectives are (a) to gain an understanding of the role of microorganisms for biotechnology applications and (b) to review advances in genetics and molecular biology of industrial microorganisms, enzyme engineering, environmental microbiology, food microbiology and molecular biotechnology. S/N 1. Microbes and cell cultivation Topics Lecture hours 6 Lee YK 2. Genetic and molecular biology of industrially important microorganisms; screening of primary and secondary metabolites Industrial biotechnology Pharmaceutical Chemical Food biotechnology 4 Lee YK 5. Diagnostic and vaccine development 6 Jimmy Kwang Total Lectures: 24h Tutorials: 8h Practicals: 6x3= 18h Total hours: 50h 4 Sim TS 2 2 Sim TS 4.

3.

TEXT BOOK (Reference books): Lee YK. Microbial Biotechnology, Principles and Application, 2nd ed. World Scientific, Singapore. 2006 MODE OF ASSESSMENT: 30% - Continuous Assessment 70% - Written Examination MODULE CO-ORDINATOR: A/P Lee Yuan Kun LECTURERS: A/P Lee Yuan Kun A/P Sim Tiow Suan Prof Jimmy Kwang

(Tel: 6516-3284, E-mail: micleeyk@nus.edu.sg)

(Tel: 6516-3284, E-mail: micleeyk@nus.edu.sg) (Tel: 6516-3280, E-mail: micsimts@nus.edu.sg) (E-mail: kwang@tll.org.sg)

52

SEMESTER II LSM3243 MOLECULAR BIOPHYSICS

Prerequisite: LSM1101 Workload: 26 lecture hours + 24 tutorial hours This module provides physical bases of macromolecular conformations and conformational stabilities under various conditions. This module also introduces basic principles and applications of a number of biophysical techniques that are commonly used for the studies of structure, dynamics and interactions of biomolecules. Specific topics include conformation of biological macromolecules, interactions (forces) that determine biomolecular structures, protein folding, protein-ligand interaction, biological membrane, and biophysical techniques. S/N 1. Topics Conformational Analysis and Forces that determine structures of protein and nucleic acid Potential energy, hydrogen bonding, hydrophobic , ion-ion , ion-dipole and dipole-dipole interactions, disulfide bonds Base pairing, base stacking Protein and DNA conformations Protein folding & Protein interaction Conformational transitions in proteins and nucleic acids Protein-protein and protein-ligand interactions Kinetics of ligand interactions Biological membranes Lipids, micelles and bilayers, membrane, membrane protein, lipid-protein interactions Transport of small molecules across cell membrane Biophysical techniques for studies of structure, dynamics and interactions of proteins, nucleic acids and membranes Circular dichroism, Fluorescence spectroscopy, Nuclear magnetic resonance Lecture hours Yang D W 6

2.

Mok Y K 6

3.

Yang D W 6 Yang D W 8

4.

Total Lectures: 26h Tutorials : 20h Practicals: 2x2 h Total hours: 50h

TEXT BOOK (Reference books): Biophysical Chemistry, Charles R.Cantor and Paul R.Schimmel, W.H.Freeman and Company, New York, 1980. Principles of Biophysical Chemistry, Kensal E.Van Holde,W.Curtis Johnson and P.Shing Ho, Prentice-Hall International, Inc, 1998. MODE OF ASSESSMENT: Exam (65%) + CA (35%) MODULE CO-ORDINATOR: Prof Yang Daiwen LECTURERS: A/P Mok Yu-Keung, Henry Prof Yang Daiwen

(Tel: 6516-1014, E-mail: dbsydw@nus.edu.sg)

(Tel: 6516-2967, E-mail: dbsmokh@nus.edu.sg) (Tel: 6516-1014, E-mail: dbsydw@nus.edu.sg)

53

SEMESTER I & II LSM3244 MOLECULAR BIOTECHNOLOGY

Prerequisite: LSM2102 Workload: 26 lecture hours + 4 tutorial hours + 20 laboratory hours This module highlights the applications of recombinant DNA and transgenic technologies to produce useful biotechnology products (e.g.: molecular diagnostics, therapeutics, agrichemicals) or commercial processes in life science. Topics to be covered include: state-of-the-art molecular tools and systems for biotechnology; recombinant protein production and downstream processing; transgenic plants and animals as bioreactors for production of pharmaceuticals (e.g.: vaccines, antibodies); human molecular diagnostics, therapeutics and gene therapy; marine biotechnology; environmental biotechnology and its impact on human health; regulatory guidelines for biotechnology products and release of GMOs into the environment. S/N 1. 2. Topics Introduction to Molecular biotechnology Transgenic animals Transgenic animal technology (basic technology and gene targeting) Applications of transgenic animals in animal husbandry and aquaculture Applications of transgenic animals in edicine and environmental science Animal cloning, stem cells and gene therapy Regulatory issues on GMOs Transgenic plants (as bioreactors for biomedical & industrial applications) Plant cell technology Plant transformation: DNA delivery systems Production and applications of transgenic plants Recombinant protein production in eukaryotic hosts/microbes Strategies for gene cloning and recombinant protein productions Advantages and limitations of prokaryotic expression systems Processing of products by filtration, centrifugation, precipitation Chromatography methods for processing biotechnology products Scale up of cell culture/microbial fermentation processes Molecular diagnostics & drugs Environmental biotechnology Environmental pollutants and their impact on human health Approaches and issues in environmental biotechnology Biodegradation, biofuels and bioremediation Total Lectures: Tutorials: Practical: Total hours: 22 h 8h (Tutorials will be given by all staff) 4h x 5 = 20h (All staff will be involved in practical) 50 h Lectures SI S II 1 L (JS) 1L (Gong) 7 L, 1.5 P 7 L, 1.5 P (GR) (Gong)

3.

6 L, 1.5 P ( XJ)

6 L, 1.5 P ( Yu)

4.

6L (JS) 2P (JS)

6L (JS ) 2P (JS)

5.

2 L (XJ)

2L (Yu)

TEXT BOOK: Glick B.R. and J.J. Paeternak (2003) Molecular Biotechnology, 3rd ed, ASM Press, Washington DC. MODE OF ASSESSMENT: CA (lab reports): 25%, Final Exam: 75% MODULE CO-ORDINATOR: Semester I A/P J.Sivaraman Semester II Prof Gong Zhiyuan LECTURERS & GUEST LECTURERS: Semester I A/P J.Sivaraman (JS) A/P Ge Ruowen (GR) Dr Xu Jian (XJ)

(Tel: 6516 1163, E-mail: dbsjayar@nus.edu.sg) (Tel: 6516-2860, E-mail: dbsgzy@nus.edu.sg)

Semester II Prof Gong ZY (Gong) Prof Yu H (Yu) A/P J. Sivaraman (JS)

54

SEMESTER I LSM3252 EVOLUTION AND COMPARATIVE GENOMICS

Prerequisite: LSM1103 Workload: 26 lecture hours + 6 tutorial hours + 18 practical hours Presentation of the theory of Evolution as one of the unifying disciplines in biology. The module introduces the three main themes in modern Evolutionary Biology: (1) Tree-of-Life: including processes and mechanisms of speciation, (II) Natural selection: including sexual selection and kinship selection, (III) Neutral Evolution: including genetic drift. Comparative Genomics is incorporated in the module by discussing the origin of life, whole genome comparisons, gene duplication, and using molecular data for the reconstruction of the phylogenetic relationships between species. The module emphasizes the importance of evolutionary biology as a tool that can explain a wide variety of phenomena in biology. S/N 1. Topics Proximate and ultimate causation in Biology Speciation and Species, Tree-of-Life, Fossil record Introduction into Phylogenetics Human Evolution: Fossil Evidence Darwinian Medicine Behavorial Evolution I & II Natural Selection I - Theory & Experimental Evidence Artificial Selection Misconceptions about Natural Selection Individual vs. Group Selection Micro & Macroevolution Kinship Selection Sexual Selection Comparative Genomics: Genetic Drift and Neutral Evolution Comparative Genomics: Molecular Clock Plant Evolution I & II Lecture hours 22 Ng Ngan Kee

2.

4 Benito Tan 26h 6x3h 6h 50h

Lectures: Practicals: Tutorials: Total hours:

RECOMMENDED TEXTBOOK: Stearns, S.C. and R.F. Hoekstra. 2005. Evolution: An Introduction. Oxford University Press, 381 pp. REFERENCE BOOKS: Andersson, M. 1994. Sexual Selection. Princeton University Press, Princeton. Dawkins, R. 1989. The Selfish Gene. Reprinted from 1976 edition. Oxford: Oxford University Press. Dawkins, R. 1986. The Blind Watchmaker. Oxford: Oxford University Press. Dawkins, Richard. 1989. Extended Phenotype: The Long Reach of the Gene. Oxford University Press. Futuyma, D. 1998. Evolutionary Biology, 3rd edition. Massachusetts: Sinauer Associates Inc. Maynard Smith, J., and Szathmry, E. 1999. The origins of life: From the birth of life to the origin of language. Oxford University Press, Oxford. Page, D. M., and Holmes, E. C. 1998. Molecular Evolution. A Phylogenetic Approach. Blackwell, Science, London. PRACTICALS and TUTORIALS: The laboratory sessions will comprise 2 field trips during which we will discover how evolution works based on living examples from animals and plants. There will be one practical session on human evolution and two practical sessions on evolutionary behavior analyzing the behavior of different animals from the evolutionary perspective. The last practical will be for the students to present their own research on a chosen topic this will be in the form of an oral session. The tutorials are incorporated into the practicals and field trips and allow the students to discuss evolution with the TAs and staff.

55

MODE OF ASSESSMENT: 40% CA; 60% Final Exam. There will be a formal written final examination (essay and short questions) which will constitute 60% of the total. The other 40% will be from one Evolutionary Behavior laboratory report, one oral presentation, and classroom participation during the practicals and on IVLE. The details are published on IVLE. MODULE CO-ORDINATOR: Dr Ng Ngan Kee LECTURER: Dr Ng Ngan Kee GUEST LECTURERS: Assoc Prof Benito C. Tan Mr N Sivasothi

(Tel: 6601-1091, E-mail: dbsngnk@nus.edu.sg)

56

SEMESTER I LSM3254 ECOLOGY OF AQUATIC ENVIRONMENTS

Prerequisites: LSM2251 Workload: 26 lecture hours + 24 tutorial/practical hours Aquatic environments make up >70% of the Earths surface. They host a huge diversity of life and ecosystems, many of which are vital to man. Topics covered in this module include the diversity and ecology of freshwater and marine habitats and organisms, the impacts of humans on these environments, and the conservation and management of these critical resources. Overall learning outcomes include an appreciation and understanding of aquatic habitats, their physical and biological properties and their associated ecosystems. The importance of both marine and freshwater environments to Singapore will be highlighted. S/N 1. 2. Lecture hours Topics Freshwater/Marine: Introduction: Course overview; linking freshwater and marine 2 biology Freshwater: Topics covered will include: 10 - Ecological characteristics of freshwater - A brief survey of freshwater environments including natural lotic (e.g., streams) and lentic (e.g., lakes) environments, and artificial or modified environments (e.g., urban habitats such as canals and reservoirs) and species diversity in these environments - Population and community ecology in freshwater environments - Ecology of freshwater ecosystems Marine: Topics covered will include: 10 - Estuaries and the interface between freshwater and marine systems. - Introduction to oceanography and the marine environment - Plankton and primary productivity - Intertidal (rocky shore and soft sediments) - Coral reefs, sea grasses and mangroves Freshwater/Marine: Conservation and management of aquatic environments; 4 course review Total Lectures: 26h Practicals + Tutorials: 24h Total hours: 50h

3.

4.

REFERENCES (Recommended text *): Freshwater *Dodson, S., 2004. Introduction to Limnology. McGraw-Hill. Dodds, W. K., 2002. Freshwater Ecology: Concepts and Environmental Applications. Academic Press. Dudgeon, D. (ed.), 2008. Tropical Stream Ecology. Academic Press. Ng, P. K. L. Ng, 1991. A Guide to the Freshwater Life in Singapore. Singapore Science Centre, Singapore. 162 pp. Yeo, D. C. J,, Wang, L. K,, Lim, K. K. P., 2010. Private Lives: An Expos of Singapores Freshwaters. Raffles Museum of Biodiversity Research. Marine *Castro, P. & Huber, M. Marine Biology. McGraw Hill. Milne, D. Marine Life and the Sea. Wadsworth. Sumich, J. An Introduction to the Biology of Marine Life. McGraw-Hill. Freshwater and marine Chuang, S. H. (ed.). 1973. Animal Life and Nature in Singapore. Singapore University Press. rd Tan, H. T. W., L. M. Chou, D. C. J. Yeo & P. K. L. Ng, 2010. The Natural Heritage of Singapore, 3 Edition. Pearson Prentice Hall. MODE OF ASSESSMENT: Continual assessment 60%; final open-book exam 40% MODULE CO-ORDINATOR: Dr Peter Todd LECTURER: Dr Darren Yeo Dr Peter Todd

(Tel: 6516-1034, Email: dbspat@nus.edu.sg) (Tel: 6516-2709, Email: dbsyeod@nus.edu.sg) (Tel: 6516-1034, E-mail: dbspat@nus.edu.sg)

57

SEMESTER II LSM3255 ECOLOGY OF TERRESTRIAL ENVIRONMENTS

Prerequisite: LSM2251 Workload: 26 lecture hours + 6 tutorial hours + 18 practical hours This module will introduce students to the principles of terrestrial ecology, with a focus on tropical forests in Southeast Asia. Major topics will include diversity and distributions of terrestrial vegetation types, soils and nutrient cycling, animal-plant interactions (pollination, seed dispersal, herbivory), disturbance ecology and succession, energy flow and food webs, and population biology. Emphasis will be placed on how people are influencing these ecological processes through their activities (e.g. land-use changes, logging, hunting, fragmentation, climate change). S/N 1 2 3 4 5 6 7 8 9 10 11 12 13 Topics Biogeography of terrestrial vegetation Species diversity: patterns and mechanisms Foods and feeding Energy and nutrients Phenology Reproductive biology: pollination and seed dispersal Population ecology Invasive species Disturbance and succession Fragmentation ecology Mangrove ecology People and tropical forest conservation Climate change and terrestrial tropical ecology Lecture hours 2 2 2 2 2 2 2 2 2 2 2 2 2 Total Lectures: 26 h Practicals + Tutorials: 24h Total hours: TEXT BOOK (Recommended text): Corlett, R.T. 2009. The Ecology of Tropical East Asia. Oxford University Press, UK. MODE OF ASSESSMENT: CA (field trip and lab reports): 50% Final Exam: 60% (MCQ and essay questions, closed book): 50% MODULE CO-ORDINATOR: Dr Edward L. Webb LECTURER: Dr Edward L Webb Prof Richard T Corlett 50h

(Tel: 6516-4184, E-mail: dbswel@nus.edu.sg)

(Tel: 65164184, E-mail: dbswel@nus.edu.sg) (Tel: 6516-1285, E-mail: dbscrt@nus.edu.sg)

58

SEMESTER II LSM3256 TROPICAL HORTICULTURE

Prerequisite: LSM1103 and LSM1104 (Not eligible to enrol without passing both modules) Workload: 26 lecture hours + 18 practical hours + 6 tutorial hours This module introduces students to the fundamentals of tropical horticulture, with emphasis on the situation in Singapore, a tropical garden city. Topics include plant growth and development and factors affecting them, pests and diseases and their control, growing media, plant nutrition, tropical urban horticulture of ornamentals, vegetable and fruit crops, native ornamental plants, vertical and roof greening, turf grass management, landscape design, organic methods and impact of horticulture on conservation. Self-directed field trips and projects will enable students to enjoy hands-on experience in tropical horticulture. S/N 1. 2. 3. 4. 5. Topics Course overview; underlying science; importance of horticulture; horticulture industry in Singapore and overseas; horticultural societies, institutions, companies Protecting horticultural plants against pests, diseases, disorders, weeds Propagation of horticultural plants and asexual reproduction; traditional methods and biotechnology Indoor plants Outdoor plants Cut flowers Ornamentals (exotic and native species) Vegetables and fruits (exotic and native species; organic and traditional methods) Turf Special techniques Non-soil growing media or methods (hydroponics, aeroponics, biochar, etc.) Urban farming Bonsai, terrariums, floral arrangements, aquatic plants Vertical and roof greening Landscape design Horticulture, conservation and environmental services in urban areas Lecture hours 2 2 2 2 6

6.

7. 8.

2 2

Total Lectures: 26h Practicals: 18h Tutorials: 6h Total hours: 50h

TEXT BOOK (Recommended texts): Boo, C. M., Kartini Omar-Hor & C.L. Ou-Yang, 2006. 1001 Garden Plants of Singapore. 2nd Edition. National Parks Board, Singapore. 780 pp. Tee, S.P. (facilitator and ed.), 2009. Trees of Our Garden City. 2nd Edition. National Parks Board, Singapore. 383 pp. MODE OF ASSESSMENT: 60% Continual Assessment (open book; assignment[s] and/or test[s]), and 40% Semestral Examination (Open Book) MODULE CO-ORDINATOR: Assoc. Prof. Hugh T.W. Tan LECTURER: Assoc. Prof. Hugh T.W. Tan

(Tel: 6516-2717, E-mail: dbsttw@nus.edu.sg)

(Tel: 6516-2717, E-mail: dbsttw@nus.edu.sg)

59

SEMESTER I LSM3261 LIFE FORM AND FUNCTION

Prerequisites: LSM1103 and either LSM1104 or LSM1201 (Not eligible to enrol without passing both modules) Workload: 26 lecture hours + 6 tutorial hours + 18 laboratory hours This module explores the complex and diverse forms and functions of plants and animals. Each living group shares a common basic structural plan, but contains many members that deviate from the basic design in response to selection pressure. Knowledge of organismal biology is enhanced through selected topics in morpho-anatomical designs and functional adaptations. S/N 1. Topics Introduction Overview on the course structure and content The diversity of life; five kingdoms Kingdom Animalia and Kingdom Plantae Morphology (in a broad sense); morphology (in a narrow sense); anatomy Functional aspects (adaptations for protection against predation, for feeding, for gas exchange and respiration, for movement and locomotion, for dispersal and reproduction, and physiology, etc.) Form and Function of Plants Plant structure, growth, and differentiation Leaf structure and function Stems and plant transport Roots and mineral nutrition Reproduction in flowering plants Plant growth responses and regulation of growth Form and Function of Animals Animal symmetry and basic designs. Unity in diversity. Animal form and function in relation to: o Protection, support, locomotion including vertebrate flight o Sensing the environment, including echolocation o Securing food o Other structural adaptations for survival Lecture hours 2 Hugh Tan

2.

12 Hugh Tan

3.

12 N. Sivasothi

Total Lectures: 26h Tutorials: 6h Practicals: 63= 18h Total hours: 50h

TEXT BOOK: The main text is: Solomon, E., L.R. Berg and D.W. Martin, 2010. Biology, 9th edition. Thomson/Brookes-Cole, Belmont, California.QH308.2 Sol 2010. The supplementary texts are: Hickman, C.P., L.S. Roberts, A. Larson, H. l'Anson and D.J. Eisenhour, 2011. Integrated principles of Zoology. 15th edition. McGraw Hill. QL47.2 Hic 2011. Pough, F. H., C. M. Janis, J. B. Heiser., 2009. Vertebrate life. 8th edition. Benjamin Cummings, San Francisco. QL605 Pou 2009. MODE OF ASSESSMENT: 40% Continual Assessment (open book assignments and/or tests) and 60% Semestral Examination (open book). MODULE CO-ORDINATOR: A/P Hugh Tan Tiang Wah LECTURERS: A/P Hugh Tan Tiang Wah Mr. N. Sivasothi

(Tel: 6516-2717, E-mail: dbsttw@nus.edu.sg)

(Tel: 6516-2717, E-mail: dbsttw@nus.edu.sg) (Tel: 6516-8869, E-mail: dbssn@nus.edu.sg)

60

SEMESTER II LSM3262 ENVIRONMENTAL ANIMAL PHYSIOLOGY

Prerequisite: LSM1104 or LSM1201 Workload: 26 lecture hours + 4 tutorial (presentation) hours + 20 laboratory hours This module aims to examine the physiological and biochemical adaptations of animals which permit them to thrive in diverse environments. It focuses on how animals adapt to natural (e.g. oxygen availability, salinity changes, water availability) and anthropogenic (e.g. greenhouse effect, UV radiation and oxidative stresses, xenobiotics) environmental challenges. This module hopes to offer students clues to what are the fundamental ways in which basic biological structures and functions of living systems are actively modified to allow organisms to exploit the full range of natural environments and to maintain the radically different modes of life we see in nature. Efforts will be made to teach how environmental physiology can be applied to biomedicine, agriculture, ecology and environmental conservation in the last part of the module. S/N 1. 2. Topics Introduction. Overview on the course structure and content. Earths atmosphere, and UV and oxidative stress adaptations. Reducing atmosphere, chemical evolution, evolution of oxygen and ozone, UV radiation, oxidative stress, oxidative defence. Respiratory adaptations. Ascorbic acid and ROS, water-land transition, gas solubility, transport of oxygen and carbon dioxide in the blood, respiratory pigments, comparison of gills and lung, regulation of blood pH, artificial blood. Circulatory adaptations: Heart structure, changing perfusion of tissues, cardiac performance. Hypoxic/anoxic adaptations. Redox balance, regulation of glycolytic rate, Pasteur effect, reversed Pasteur effect, mountain sickness, hypoxia and cancer. Osmoregulatory adaptations. Osmotic and ionic regulation in freshwater or seawater, euryhalinity and branchial osmoregulatory acclimation, urea as an osmolyte, aquaculture, water purification; salt gland and mammalian kidney, survival in the desert. Excretory adaptations. Ammonia. Urea. Uric acid. Water-land transition, space travel. Temperature adaptations. Adaptations to temperature extremes; global warming, green house effect. Adaptation to extreme environmental conditions--Lack of food, hypoxia, desiccation, temperature extremes. Symbiosis, hibernation and aestivation, climate changes, flooding and drought, famine. Adaptation to environmental contaminations. Acid rain, xenobiotics, hormone disruptors Total lectures : Tutorials (presentation): Practicals: Total hours: TEXT BOOK (Reference books): Research papers and review articles will be used as course materials. MODE OF ASSESSMENT: Term paper 20%; test 20%; final exam 60% Lecture hours 1 2

3.

4. 5.

1 4

6.

7. 8. 9.

2 3 3

10.

2 26h 4h 5x4=20h 50h

MODULE CO-ORDINATOR: Prof Ip Yuen Kwong (Tel: 6516-2702, E-mail: dbsipyk@nus.edu.sg) LECTURER: Prof Ip Yuen Kwong

61

SEMESTER IV LSM3263 Field Studies in Neotropical Ecosystems

Prerequisite: LSM 2251 Ecology and the Environment Workload: 60 lecture hours + 24 tutorial hours + 36 laboratory hours + 36 hours of fieldwork/projects/assignments + 24 preparatory hours An intensive six-week summer program conducted with the University of Costa Rica. The first two weeks will be spent on campus in San Jose, with lectures and tutorials on comparative paleotropical (Singapore) and neotropical (Costa Rica) terrestrial and marine ecosystems; comparative urban ecology as well as comparative conservation issues and policies. The latter four weeks will be spent at research stations located in the various ecosystems; the cloud forest, neotropical montane forest, tropical Caribbean coastal forest and coastal Pacific ecosystem. Students are exposed to different techniques in field biology and will be trained in forming ecological hypotheses while in the field. Lectures, student projects and student assessments will be carried out at each field site. TEXT BOOK (Reference books): Janzen DH (1983) Costa Rican Natural History. University of Chicago Press. Tan HTW, LM Chou, DCJ Yeo and PKL Ng (2010) The Natural Heritage of Singapore. Pearson. Sodhi NS, BW Brook & CJA Bradshaw (2007) Tropical Conservation Biology. Blackwell Publishing. Supplementary readings: Nadkarni NM & NT Wheelwright (2000) Ecology and Conservation of a Tropical Cloud Forest. Oxford University Press. Corlett RT & RB Primack (2011) Tropical Rain Forests: An Ecological and Biogeographical Comparison, 2nd Edition. Wiley-Blackwell, Oxford, UK. MODE OF ASSESSMENT: 100% continuous assessment. MODULE CO-ORDINATOR & LECTURER: Dr Zeehan Jaafar

(Tel: 6601-1456; E-mail: dbszj@nus.edu.sg)

62

SEMESTER II LSM3272 GLOBAL CHANGE BIOLOGY

Prerequisite: LSM1103 Workload: 26 lecture hours + 24 tutorial/practical hours This module will introduce students to aspects of current environmental change and its effects on biological systems, including rising carbon-dioxide (CO2) concentrations, climate change, loss of biodiversity, and eutrophication. Topics covered will include the following: climate change, global warming, land-use changes, invasive species, fire, carbon sequestration, carbon mitigation and global ecology. Students will explore prevailing environmental issues in greater depth through mini projects, classroom discussions and debates. S/N 1. 2. 3. 4. 5. 6. 7. 8. 9. Introduction Habitat loss Extinctions Climate change Invasive species Fire Urban areas and climate change Global ecological analyses Protected areas and climate change Topics Lecture hours 2 4 2 4 4 2 2 4 2 Total Lectures: 26h Practicals + Tutorials: 24h Total hours: 50h

TEXT BOOK (Recommended text): Sodhi, N.S., B.W. Brook & C.J.A. Bradshaw, 2007. Tropical Conservation Biology. Blackwell, Oxford, UK. 332 pp. Sodhi, N.S. and Paul Ehrlich, 2010. Conservation Biology for All. MODE OF ASSESSMENT: 40% CA, 60% Final Examination MODULE CO-ORDINATOR: Dr Zeehan Jaafar LECTURERS: Asst Prof David Bickford Dr Posa, Mary Rose Cervantes Dr Zeehan Jaafar

(Tel: 6516- 6867, E-mail: dbszj@nus.edu.sg)

(Tel: 6516-1034, E-mail: dbsbdp@nus.edu.sg) (E-mail: dbspmrc@nus.edu.sg) (Tel: 6516- 6867, E-mail: dbszj@nus.edu.sg)

63

SEMESTER I LSM4211 TOXICOLOGY

Prerequisite: LSM3211 or LSM3221 Workload: 26 lecture hours + 16 PBL hours + 4 Revision hours Toxicology is the study of the adverse effects of chemicals on humans. This introductory course will provide the basics for quantitative risk assessment of hazardous compounds and for extrapolation from animal data to humans. It will link adverse effects at the molecular level to overall toxic responses in humans. The general concepts will be illustrated with examples including drugs, natural toxins, industrial chemicals, and environmental toxicants. S/N 1. Topics Concepts and principles of modern toxicology Lecture hours 2 Shen H-M 2. Toxicodynamics / Toxicokinetics 4 SM Moochhala 2 Shen H-M 6 SM Moochhala 2 M Bhatia 2 Shen H-M 4 Ho HK 6. Environmental toxicology Immunotoxicity/Toxicity assessment in drug discovery 2 Ong CN 7 2 Ho HK Total Lectures: 26h Revisions: 4h Problem-based Learning: 16h Total hours: MODE OF ASSESSMENT: CA/PBL 40 %; Final Exam 60 % MODULE CO-ORDINATOR: A/P Shen Han-Ming LECTURERS: A/P Shen Han-Ming A/P Shabbir Moochhala Prof. Ong Choon Nam Dr. Ho Han Kiat 46h

3.

Mechanistic toxicology

4.

Chemical mutagenesis and carcinogenesis: Genotoxicity Carcinogenesis Acute, Sub-acute and Chronic Toxicology

5.

Organ-selective toxicity

(Tel: 6516-4998; E-mail: ephshm@nus.edu.sg)

(Tel: 6516-4998; E-mail: ephshm@nus.edu.sg) (Tel: 6485-7201; E-mail: phcsmm@nus.edu.sg) (Tel: 6516-4982; E-mail: ephocn@nus.edu.sg) (Tel: 6516-7963; E-mail: phahohk@nus.edu.sg)

64

SEMESTER I LSM4212 PHARMACOGENETICS AND DRUG RESPONSES

Prerequisite: LSM3211 or LSM3221 Workload: 34 lecture hours + 15 tutorial hours + 1 hr CA This module will examine the scientific bases for all aspects of human variability in clinical responses to drugs and other xenobiotics. The course will provide both the theoretical and technical know-how to conduct and interpret simple studies relating to intraindividual, interindividual as well as interpopulational differences in drug responses. S/N 1. 2. 3. 4. Introduction to Course Measurement of Drug Response Pharmacokinetic Variability Pharmacogenetics Topics Lecture hours 2 2 8 4

5. 6. 7. 8. 9.

Race/Ethnicity/Population Issues Non- Genetic Factors in Variability Genetic Factors in Variability Drug Metabolism - Cause Of Variability Drug Transporters

2 2 2 8 4 Total Lectures: 34h Tutorials & Coursework: 15h CA: 1h Total hours: 50h

TEXTBOOKS/REFERENCE TEXTS: Pharmacogenomics / edited by Werner Kalow, Urs A. Meyer, Rachel Tyndale. Imprint New York : Marcel Dekker, 2001. Pharmacogenomics : the search for individualized therapies / edited by Julio Licinio and Ma-Li Wong. Imprint Weinheim : Wiley-VCH, c2002. Clinical Pharmacokinetics : concepts and applications / Malcolm Rowland, Thomas N. Tozer. Imprint Baltimore : Williams & Wilkins , c1994. Edition 3rd ed. Pharmacokinetics made easy / Donald J. Birkett. Imprint Sydney ; New York : McGraw Hill, c1998. MODE OF ASSESSMENT: Continuous Assessment and Final Examination MODULE CO-ORDINATOR: Prof Lee, Edmund J D LECTURER IN-CHARGE: Prof Edmund Lee

(Tel: 6516-3677, E-mail: phcelee@nus.edu.sg)

(Tel: 6516-3677, E-mail: phcelee@nus.edu.sg)

65

SEMESTER I LSM4213 SYSTEM NEUROBIOLOGY

Prerequisite: LSM3213 Workload: 28 lecture hours + 10 Tutorial hours +12 Self-Directed Learning (SDL) hours The primary goal of this module is to understand how (a) neurons, assembled into circuits, mediate behavior and (b) the pathophysiology of neurons leading to dysfunctional cellular and molecular processes and behavior. This course draws on basic knowledge of the cell biology and physiology of neurons.

S/N 1.

Topics

Proposed Lectures 10 Khanna S (Sem1)

Sensory & Motor Functions 1. Functional neuroanatomy (2hr) 2. General scheme of sensory processing (1hr) 3. Somatosensation-pain (2hr) 4. Vision (2hrs) 5. Organizational features of motor processing (1hr) 6. Planning, execution & co-ordination of movement (2hr) 2. Higher Brain Functions 7. Behavior & gene (1hr) 8. Neural plasticity (2hr) 9. Higher order brain functions (1hr) 10. Memory systems (1hr) 11. Mechanisms of learning & memory (3hrs) 12. Assay systems in cognitive studies (2hr) 3. Aging and neurodegeneration 13. Neurobiology of aging (2hrs) 14. Neurodegenerative diseases Mechanisms & Pathogenesis (1hr) 15. Alzheimers disease (2hrs) 16. Parkinsons and other brain diseases (1hrs) 15. Therapies for Brain Repair (2hrs)

10 Lee A (Sem1)

8 Wong BS (Sem1)

Total Lectures: 28 hrs Tutorials: 10 hrs SDLs: 12 hrs

Total hours:

50h

TEXT BOOK: Bear MF, Connors BW, Paradiso MA, (eds.), Neuroscience: Exploring the Brain, 3rd edition MODE OF ASSESSMENT: 45% CA (reports based on SDL), 55% Final Exam MODULE CO-ORDINATOR: Dr. Wong Boon Seng LECTURERS: A/P Sanjay Khanna Dr Wong Boon Seng Dr Lee Yiu-Wah

(Tel: 6516-7617, E-mail: phswbs@nus.edu.sg)

(Tel: 6516-3665; E-mail: phsks@nus.edu.sg) (Tel: 6516-7617, E-mail: phswbs@nus.edu.sg) (Tel: 6516-3668, E-mail: phsleeyw@nus.edu.sg)
66

SEMESTER II LSM4214 CANCER PHARMACOLOGY

Prerequisite: LSM3211 and LSM4243 (LSM4243 can be read as co-requisite) Workload: lecture hours + tutorial hours This module will introduce students to the general principles of drug actions that underpin their therapeutic applications against cancers, from conventional (non-specific) chemotherapy to target-specific drugs. It will provide details of drugs used in specific cancer types, ranging from those with proven efficacy in clinics (e.g. Gleevec) to experimental agents in trials. Conceptual and theoretical targets (e.g. RNAi and gene therapies) will also be introduced. S/N 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 Topics Cancer overview: from biology to etiology and epidemiology Different cancer types and current treatment methods Conventional chemotherapeutic drugs and associated toxicities Drugs against hormone-related cancer - nuclear receptor and HDAC Anti-inflammatory drugs - chemoprevention and NFkB inhibitors Drugs targeting cancer signaling pathways Molecular targeted therapies Models for drug target discovery and evaluation Oncogenes and gene therapies Cancer biotherapeutics Cancer pharmacogenomics and personalized medicines Small group tutorials : topics 1-2 Small group tutorials : topics 3-4 Small group tutorials : topics 5-6 Small group tutorials : topics 7-8 Lecture hours 2 2 2 2 2 2 2 2 2 2 2 2 2 2 2 Total Lectures: 22 h Tutorials & Coursework: 8 h Seminar & Project : 12 h Self-directed learning : 8 h Total hours: TEXTBOOKS/REFERENCE TEXTS: Anticancer drug development Author: Bruce C. Baguley, David J. Kerr Cancer chemotherapy and biotherapy: principles and practice Author: Bruce A. Chabner, Dan L. Longo Molecular cancer therapeutics: strategies for drug discovery and development Author: George C. Prendergast Medicinal chemistry of anticancer drugs Author: Carmen Avendao and J. Carlos Menndez. Avendao Cancer drug resistance Author: Beverly A. Teicher
67

50 h

- / 2002 Academic ISBN: Press - / 2006 Lippincott ISBN: Williams & Wilkins - / 2004 Wiley-Liss ISBN: - / 2008 Elsevier ISBN:

Compulsory Compulsory

Compulsory

Supplementary

- / 2006 Humana Press Supplementary ISBN:

Cancer: Principles and Practice of Oncology Author: DeVita, Hellman & Rosenberg ONLINE RESOURCES: MedlinePlus on Cancer: http://www.nlm.nih.gov/medlineplus/cancer.html ASCO All About Cancer: http://www.cancer.net/patient/All+About+Cancer MODE OF ASSESSMENT: EXAMINATION (MCQ & Long Essays) MID-TERM TEST (Short & Long Essays) SEMINAR PRESENTATION MODULE CO-ORDINATOR: A/P John Luk LECTURERS A/P John Luk Dr Gautam Sethi Dr Alan Prem Kumar Dr Eliana C Martinez Valencia 50% 35% 15%

(E-mail: jmluk@nus.edu.sg)

(E-mail: jmluk@nus.edu.sg) (E-mail: phcgs@nus.edu.sg) (E-mail: nmiapk@nus.edu.sg) (E-mail: surecmv@nus.edu.sg)

68

SEMESTER II LSM4221 DRUG DISCOVERY AND CLINICAL TRIALS

Prerequisite: LSM3211 or LSM3221 Workload: 31 lecture hours + 6 group work hours + 9 project/ seminar hours +4 self directed learning hours This module will cover the stages that a drug that is developed for clinical use goes through before it is marketed: discovery/synthesis, preclinical studies, clinical drug trials, registration and post-market surveillance. The different phases of clinical drug trials and the guidelines for ethics and good clinical practice will be discussed. Students are also divided into groups to discuss and design clinical trials. At the end of the course the students will have an overview of the processes involved in bringing a drug from the laboratory to the market. S/N 1. Introduction: History and Overview Drug discovery/Nonclinical development (NCD) - ADME profiling and Safety assessment Clinical Drug Trials Good Clinical Practice/Ethics Topics Lecture Hours 1 8

2.

3.

4.

5.

Drug Regulatory Authority/Registration

6.

Good Manufacturing Practice

7.

Group Work

8.

Clinical trial project/Presentation

Total Lectures: 31h Group Work : 6h Projects/Seminar: 9h Self Directed Learning: 4h Total hours: MODE OF ASSESSMENT: Continuous Assessment and Final Examination MODULE CO-ORDINATOR: A/P Tan Chay Hoon LECTURER IN-CHARGE: A/P Tan Chay Hoon 50h

(Tel: 6516-3310, E-mail: phctanch@nus.edu.sg )

(Tel: 6516-3310, E-mail: phctanch@nus.edu.sg )

69

SEMESTER I & II LSM4222 ADVANCED IMMUNOLOGY

Prerequisite: LSM3223 Workload: 28 lecture hours + 22 tutorial hours The objective of this course is to provide students with a current and up to date view of immunology. Breakthrough areas will certainly vary from year to year, but the broad subject matter will remain. Among the highly competitive areas of immunology research, the course focuses on innate immunity, dendritic cell biology, antigen processing and presentation, lymphocyte development and differentiation, induction of tolerance, mechanism of autoimmunity and allergy, host pathogen interactions and tumor immunology. Lecture S/N Topics hours 1. Innate immunity complement activation, antimicrobial peptides, and phagocytes in innate immunity. Phagocytic receptors and mechanisms of pathogen killing inside phagosomes. 2. Toll-like receptors overview, structure, specificity, signaling and adjuvant role in the induction of adaptive immunity. 8 1. 3. Dendritic cells immunobiology and activation. 4. Natural killer cells NK activation, NK cell mediated cytotoxicity, and NK cell cross-talk with other cells. 5. Inflammation molecular and cellular mechanisms, cytokines, chemokines, leukocyte adhesion/migration. 6. Lymphocyte development - hematopoeitic stem cell biology, the development of central tolerance. 7. Antigen presentation - antigen processing, antigen loading, MHC binding selection, and 2. cross presentation. 12 8. T cell subsets Th1, Th2, Th17 and Treg differentiation, molecular characteristics, effector mechanisms. 9. Mucosal immunity 10. Tumor immunology 11. Allergy 8 12. Host-pathogen interactions Tutorials: Each group of students will be assigned a cutting edge journal article from a topic for oral presentation. There will be a maximum of 10 groups. Tutorial hours include a class practice 22 session on critical reading of literature and art of writing critiques, group presentations and class discussion. Total Lectures: 28h Tutorials: 22h Total hours: 50h

3.

4.

READING MATERIAL: Journal articles and Reviews will be provided. MODE OF ASSESSMENT: 30% Continuous Assessment 70% Final Examination MODULE CO-ORDINATORS: (Semester I) Dr Veronique Angeli (Semester II) A/P Gan Yunn Hwen LECTURERS : Semester I Dr Veronique Angeli (MC) A/P Ren Ee Chee Dr Stephan Gasser

(Tel: 6516-7207; E-mail. micva@nus.edu.sg) (Tel: 6516-3678; E-mail: bchganyh@nus.edu.sg)

Semester II A/P Gan Yunn Hwen (MC) Dr Wong Siew Heng A/P Herbert Schwarz
70

SEMESTER II LSM4223 ADVANCES IN ANTIMICROBIAL STRATEGIES

Prerequisite: LSM3232 or LSM3225 Workload: 16 lecture hours + 8 team presentation hours + 21 practical hours + 5 tutorial/consultation hours An advanced course in the study of infectious diseases of man with emphasis on new and emerging infections as well as those of major clinical/economic importance. Core topics include understanding the principles and practice of Medical Microbiology, the nature and emergence of antimicrobial resistance, antimicrobial strategies, changing epidemiology of infections and laboratory diagnosis using classical diagnostic techniques and current molecular approaches. Seminars will be conducted as team presentations to explore current topics on infectious diseases in depth. A strong practical component is included. S/N 1.

2.

3.

4.

5.

6.

7.

8.

Topics Introductory overview of contemporary Medical Microbiology. Laboratory methods of diagnosis. The classical methods of diagnosis microscopy, culture and serology. The contribution of molecular diagnostics to modern laboratory practice. Genomics and proteomics of microbial pathogens. Immunology and infection. A brief overview of the immune defence mechanisms against infection, innate and adaptive. Detailed consideration of specific mechanisms by which microbes are recognized and killed. Microbial pathogenesis. Attachment, invasion, toxins, immunopathogenesis and immune avoidance mechanisms. The molecular analysis of these phenomena will be emphasized. Opportunistic infections. The broad range of opportunistic pathogens which infect immunocompromised patients, difficulties in their identification, and the interpretation of their significance. Evolution and spread of antibiotic resistance genes. The mechanisms of action of antimicrobial agents, the molecular basis of resistance, and the genetics of resistance acquisition and spread. New and new infections. Recently recognized pathogens, new variants of known pathogens (e.g. multiple-resistant variants), and the contribution of infectious agents to diseases not previously perceived as having any microbial cause. Immunization. Old and new vaccines. New methodologies like DNA vaccines, novel methods of delivery and improved adjuvant design. Total Lectures: Tutorials: Team Presentations: Practicals: Total hours:

Lectures 1

2 16h 5h 8h 21h 50h

TEXT BOOK: Fundamentals of Microbiology, 8th edition, Alcamo (Jones & Bartlett). Jawetz, Melnick & Adelberg's Medical Microbiology, Lange Medical Books. Medical Microbiology, 4th edition, Baron S (Editor), University of Texas Medical Branch Available free on-line www.ncbi.nlm.nih.gov/bookshelf/br.fcgi?book=mmed) REFERENCE TEXTS: Bacterial Pathogenesis: A Molecular Approach, 2nd edition, Salyers AA & Whitt DD, ASM Press. Infectious Disease, 2nd edition, Bannister BA, Begg NT & Gillespie SH, Blackwell Science. MODE OF ASSESSMENT: 40% - Continuous Assessment 60% - Final Examination
71

MODULE CO-ORDINATORS: A/P Vincent Chow Dr Kevin Tan

(Tel: 6516-3691, E-mail: micctk@nus.edu.sg) (Tel: 6516-6780, E-mail: mictank@nus.edu.sg)

LECTURERS: (Teams I and II will teach in alternate Academic Years): Team 1 Team 2 Guest Lecturers A/P Vincent Chow Dr Kevin Tan Dr Gamini Kumarasinghe A/P Tan Yee Joo A/P Tan Yee Joo A/P Laurent Renia (SIgN. A*STAR) Dr Sylvie Alonso Dr Sylvie Alonso Dr Thierry Diagana (NITD)

72

SEMESTER II LSM4224 FREE RADICALS & ANTIOXIDANT BIOLOGY

Prerequisite: Life Sciences major Workload: 26 lecture hours + 10 presentation hours + 14 self directed learning/ preparation hours This module examines the role of free radicals and antioxidants in human health and disease, with a focus on molecular and cellular aspects. Topics covered include free radical chemistry, antioxidant defences, their role in normal metabolism, cardiovascular and neurodegenerative diseases, cancer and the ageing process. At the end of this module students should be able to critically evaluate current literature in this area. S/N 1. 2. 3. 4. 5. 6. 7. 8. 9. 10. 11. 12. Topics Chemistry sources and detection of free radicals Antioxidant defences derived from the diet Antioxidant defences: endogenous Measuring Oxidative Damage Free radicals as useful species; signal transduction and phagocytosis Free radicals and apoptosis Neurodegenerative Disease Atherosclerosis Transition metal metabolism in relation to free radical damage DNA damage, Infertility, Cancer Free Radicals and Toxicology Diabetes Total Lectures: Presentations: Preparation/SDL: Total hours: Lecture hours 2 4 2 2 2 4 2 2 2 2 2 2 28h 8h 14h 50h

TEXT BOOK: Free Radicals in Biology and Medicine (B. Halliwell and J.M.C. Gutteridge) Edition 4, Oxford University Press MODE OF ASSESSMENT: Continual Assessment (35%) Final Examination (65%) MODULE CO-ORDINATOR: Prof Barry Halliwell LECTURERS: Prof Barry Halliwell A/P Walczyk Thomas Rainer A/P Ong Wei Yi A/P Tan Kwong Huat, Benny A/P Marie-Veronique Clement A/P Shen Han-Ming Dr Jan Gruber

(Tel: 6516 3247, E-mail: bchbh@nus.edu.sg)

(Tel: 6516 3247, E-mail: bchbh@nus.edu.sg) (Tel: 6516 7986, E-mail: chmwtr@nus.edu.sg) (Tel: 6516 3662, E-mail: antongwy@nus.edu.sg) (Tel: 6516 3272, E-mail: phctankh@nus.edu.sg) (Tel: 6516 7985, E-mail: bchmvc@nus.edu.sg) (Tel: 6516 4998, E-mail: cofshm@nus.edu.sg) (Tel: 6516 4638, E-mail: bchjg@nus.edu.sg)

73

SEMESTER II LSM4225 GENETIC MEDICINE IN THE POST-GENOMIC ERA

Prerequisite: LSM2102 Workload: 27 lecture hours + 23 presentation/self-directed learning/preparation hours This module is intended to provide a good foundation and stimulate students interest in specialized topics in Genetics and Genomics related to translational research. The module will provide students with knowledge of current practices in Genetic Medicine. Students will also know how gene identification, diagnostic and therapeutic strategies are formulated and performed. They will also be expected to show how to translate new genetic and genomic discoveries into novel diagnostic and therapeutic strategies. S/N 1. Introduction and Review i. Review of Human Genetics Principles ii. Mutation screening and genotyping strategies 2. Genetic Testing i. Cytogenetics ii. Molecular Genetics 3. High-Throughput / Ultra-High Throughput Strategies for Genetic Medicine i. Arrayed expression profiling ii. Massively parallel sequencing 4. 5. Identification of Disease Genes and Pharmacogenetics/Pharmacogenomics Molecular Therapy i. Cell Therapy ii. Gene Therapy 6. Summary and Conclusion i. 7. Ethical, legal, and social issues in genetic medicine 23 Total contact hours: 50h MODE OF ASSESSMENT: Projects & Presentations: 50%; Semester examination: 50% MODULE COORDINATORS: A/P Caroline Lee (Tel: 6516-3251 or 6436-8353 and bchleec@nus.edu.sg). and A/Prof Samuel Chong (Tel: 6772-4152 and paecs@nus.edu.sg). LECTURERS: A/P Caroline Lee (CGL), A/Prof Samuel Chong (SSC) and A/Prof Heng Chew Kiat (HCK) HCK, SSC & CGL 1 CGL 3 5 CGL CGL 3 CGL 10 SSC Topics Lecture Hours 5 Lecturer HCK

Tutorial, Projects and Presentations

74

SEMESTER I LSM4226 INFECTION AND IMMUNITY

Prerequisite: LSM3223 and LSM3232 Workload: 38 lecture hours + 12 tutorial hours This module aims at providing an in-depth knowledge in the field of host-pathogen interactions, i.e. how the immune system deals with pathogens, and how the pathogens deal with the hosts immune system. An introductory lecture series covers the basics in microbiology (bacteriology, virology, parasitology), immunology, vaccinology, and general principles of host-pathogen interactions. Selected diseases illustrate host-pathogens interactions along with the consequences for vaccine and drug design. The following set of lectures covered by clinicians and professionals focus on patient management, field study, as well as safety aspects when working with pathogens in a research lab. Tutorials are broken into journal club, article write-up exercise and problem-based study and are directly related to the topics developed during the lectures. S/N 1 2 3 Topics Introduction- Presentation of the module and the lecturers. Bacteriology and bacterial pathogenesis (basic principles) Virology and viral pathogenesis (basic principles) Lecture hours 2h 2h 2h

4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19

Parasitology and parasite pathogenesis (basic principles) Immunology basics (innate and adaptive immunity; mucosal immunity) Host-pathogens interactions (Immune evasion) Vaccinology (History; types of vaccines; adjuvants; neonatal vaccinology) Animal models of infection M. tuberculosis: TB and virulence strategies M. tuberculosis: Drug development against TB Plasmodium: Malaria and virulence strategies Plasmodium: Vaccine and drug development against malaria Dengue virus: DEN disease and virulence strategies Dengue virus: Vaccine and drug development against DEN TB: DOT therapy/TB vaccines DEN: How to manage DEN patients Malaria: Epidemiology studies/ sample collection Biosafety and Regulatory Aspects Animal use for studying pathogenesis Total lectures : Tutorials (presentation): Practicals: Total hours:

2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 2h 38 h 12h 0h 50h

75

TEXT BOOK (Reference books): -Microbiology- A systems Approach 2nd edition (Cowan-Talaro) McGraw-Hill international edition -Janeways Immunobiology 7th edition (Murphy-Travers-Walport) - Foundations in Microbiology 7 edition (Kathleen Park Talaro) McGraw-Hill edition - Immunology 7th edition (David Male/ J. Brostoff/ D. Roth/ I. Roitt)
th

MODE OF ASSESSMENT: Continuous assessment (30%) and final examination (70%) MODULE CO-ORDINATOR: Dr ALONSO Sylvie and Dr ANGELI Veronique LECTURER:

Name: Dr Alonso Dr Angeli Dr Zhang Dr Tan YJ Dr Tan SW (Tel: 6516 3541, E-mail: micas@nus.edu.sg) (Tel: 6516 7207, E-mail: micva@nus.edu.sg) (Tel: 6516 6407, E-mail: miczy@nus.edu.sg) (Tel: 6516 3692, E-mail: mictyj@nus.edu.sg) (Tel: 6516 6780, E-mail: mictank@nus.edu.sg)

76

SEMESTER II LSM4231 STRUCTURAL BIOLOGY

Prerequisite: LSM2103 Workload: 28 lecture hours + 8 practical hours + 14 tutorial hours This module provides an overall view on the structure determination of protein molecules, protein complexes, and viral assemblies, and molecular architectures in cell. Topics will include the theory and practice of the three major methods cryo- X-ray crystallography, electron microscopy (cryo-EM), and nuclear magnetic resonance (NMR). S/N 1. X-ray crystallography Protein crystallization Crystal symmetry X-ray diffraction Data collection Structure determination Three-dimensional electron microscopy and its applications in biology Introductions to cryo-EM Single particles analysis & electron crystallography Electron tomography & application Sample preparation and application Nuclear magnetic resonance spectroscopy Basic NMR theory Basic NMR experiments NMR resonance assignment and protein structure determination NMR sample preparation and application of NMR to dynamics and metabonomics Topics Lecture hours 8 J. Sivaraman 4 Adam Yuan

2.

8 Sheemei Lok

3.

8 Daiwen Yang

Total Lectures: 28h Practicals and Tutorials: 22h Total hours: TEXT BOOKS: NMR of macromolecules Roberts, G.C.K. Oxford University Press, 1993 X-Ray Structure Determination: A Practical Guide Stout, G.H. & Jensen, L.H. Wiley-Interscience, 2nd edition, 1989. Crystal Structure Analysis for Chemists and Biologists Glusker, J.P., Lewis, M. & Rossi, M. John Wiley & Sons, 1994. Three-Dimensional Electron Microscopy of Macromolecular Assemblies: Visualization of Biological Molecules in Their Native State, Joachim Frank, Oxford University Press, USA, 2nd edition, 2006 MODE OF ASSESSMENT: Final exam: 60 marks (X-ray-30, NMR-15, EM-15) CA: 40 marks (X-ray 20, NMR-10, EM-10) from any combination of Project-10, Assignment-10, Quiz-10, Written exam-10, Facility visit report-10 or lecturers own mode of CA-10 marks. MODULE CO-ORDINATOR: Prof Daiwen Yang, DBS LECTURERS: Prof Daiwen Yang, DBS A/P Jayaraman Sivaraman, DBS Dr Sheemei Lok, DBS Dr Adam Yuan, Temasek Life Sciences Laboratory 50h

(Tel: 6516-1014, E-mail: dbsydw@nus.edu.sg)

(Tel: 6516-1014, E-mail: dbsydw@nus.edu.sg) (Tel: 6516-1163, E-mail: dbsjayar@nus.edu.sg) (Tel: 6516-5840, E-mail: gmssl@nus.edu.sg) (Tel: 6516-7409, E-mail: adam@tll.org.sg)
77

SEMESTER I LSM4232 ADVANCED CELL BIOLOGY

Prerequisite: LSM2103 Workload: 26 lecture hours + 8 tutorial hours + 10 journal discussion hours + 6 hours for 2 CAs, 1 report This module will explore the changes that occur in animal cells as they grow, mature, differentiate, and either senesce or renew themselves. Insights into the mechanisms that govern how and when particular developmental alterations occur will be discussed. Emphasis will be placed on understanding the cellular molecular mechanisms that lend themselves to experimental manipulation. S/N 1. Topics Cell Membranes Basic components and organisation of cell membranes: Lipids and Lipid polymorphism Membrane Dynamics: Membrane folding, vesicle budding, fusion, exocytosis and endocytosis Membrane functions: Bioenergentic membranes Cell Dynamics Control Morphogenesis, Differentiation, and Development Small GTPases and their regulators as major cellular switches: Cytoskeletal networks - their interplay in cell morphogenesis, cell motility, cell division, organogenesis and development Cell differentiation - mechanisms Embryonic and postnatal myogenesis Differentiation of skeletal muscle Stem cells and Notch signalling in adult myogenesis and ageing Muscle degeneration mechanisms in muscular dystrophies Ageing and cellular senescence Oxidative stress and mitochondrial ageing o DNA o Proteins Genome-based theory of aging o Telomere shortening Aging retardation and longevity Caloric Restriction Lecture Hrs 6 Deng Yuru

2.

8 Low Boon Chuan

3.

4 Reshma Taneja

4.

8 Prakash Hande

Total Lectures: 26h Tutorials: 8h Journal Discussions: 10h 2 CAs and 1 Report: 6h Total hours: 50h MAIN REFERENCES: Selected papers SUPPLEMENTARY TEXT BOOKS: (1) Genes VII (Lewin) (2) Molecular Biology of the Cell (4th Edition by Alberts, Johnson, Lewis, Raff, Roberts & Walter; Publisher: Garland Science) MODE OF ASSESSMENT: 50% CA (2 x short essay question tests, 1 x written report/student presentations) 50% Final exam (multiple choice questions and Essay type questions, closed book) MODULE CO-ORDINATOR: A/P Prakash Hande LECTURERS: Dr. Deng Yuru A/P Low Boon Chuan A/P Reshma Taneja A/P Prakash Hande

(Tel: 6516-3664, E-mail: phsmph@nus.edu.sg)

(Tel: 6516-1935, E-mail: phsyd@nus.edu.sg) (Tel: 6516-7834, E-mail: dbslowbc@nus.edu.sg) (Tel: 6516-3236, E-mail: phsrt@nus.edu.sg) (Tel: 6516-3664, E-mail: phsmph@nus.edu.sg)
78

SEMESTER II LSM4241 FUNCTIONAL GENOMICS

Prerequisite: LSM3231 Workload: 26 lecture hours + 24 tutorial hours This module aims to introduce selected topics on functional genomics. Areas covered include: the assignment of functions to novel genes following the genome-sequencing projects of human and other organisms; the principles underlying enabling technologies: DNA microarrays, proteomics, protein chips, structural genomics, yeast two-hybrid system, transgenics, and aspects of bioinformatics and its applications; and to understand the impact of functional genomics on the study of diseases such as cancer, drug discovery, pharmacogenetics and healthcare. S/N 1. Topics Introduction Understanding the human genome project, and its significance. Post-genomic era : functional genomics and comparative genomics Gene expression analysis Pioneering methodologies: Subtractive hybridization Differential display of alteration of gene expression Lecture Hours 2

2.

12

3.

Parallel and HTS Oligonucleotide and cDNA microarrays Tissue and cell microarrays : principles and applications Functional assays/validation Mutagenesis Animal models Applications: siRNA Human disease Drug discovery SNPs Proteomics : technologies and applications Trends in instrumentation for proteomics 12 Databases and knowledge resources for proteomics research Localizome: large scale study of cellular localization of proteins Interaction proteomics ( or protein-interaction mapping for functional proteomics: use of yeast two-hybrid systems; biomolecular interactions (BIA) using surface plasmon resonance (SPR); TAP (tandem affinity purification) tag Protein interaction networks Protein chips/arrays Structural genomics High throughput protein expression and structural analysis using NMR and x-ray diffraction Applications of functional proteomics in : Disease detection (e.g. cancer) Drug discovery Total Lectures: 26 hrs Tutorials, Project Presentation and CA: 10 hrs Self-Study: 14 hrs Total hours: 50h

MODE OF ASSESSMENT: 2 CAs: 40% (20% each); Semestral examination: 60% MODULE CO-ORDINATOR: A/P Maxey Chung (Tel: 6516-3252, E-mail: bchcm@nus.edu.sg)

79

LECTURERS: Semester I A/P Maxey Chung Prof K Jeyaseelan Dr Markus Wenk Semester II A/P Maxey Chung Dr Ng Huck Hui Dr Han Jin-Hua

(Tel: 6516-3252; E-mail: bchcm@nus.edu.sg) (Tel: 6516-3248; E-mail: bchjeya@nus.edu.sg) (Tel: 6516-3624; E-mail: bchmrw@nus.edu.sg)

(Tel: 6516-3252; E-mail: bchcm@nus.edu.sg) (Tel: 6478-8145; E-mail: dbsnghh@nus.edu.sg) (Tel: 6516-2694; E-mail: dbshjh@nus.edu.sg)

80

SEMESTER I LSM4242 PROTEIN ENGINEERING

Prerequisite: LSM3244 Workload: 26 lecture hours + 24 hours of tutorial and discussions This module will familiarize students with the technologies that can be used to produce and engineer various proteins for basic biological research and biotechnology applications. The fundamental principles for manipulating protein production as desired and the common expression systems will be presented. The emphasis will be on the experimental strategies and approaches to improve protein properties and to create novel enzymatic activities. The topics include gene expression and protein production systems, uses of gene fusions for protein production and purification, directed molecular evolution and DNA shuffling, and engineering of proteins and enzymes for improved or novel properties. Some specific examples in protein engineering will be highlighted. S/N 1. Topics Lecture Hours Introduction Prokaryotic and eukaryotic systems for protein production 4 h (Pan SQ) Strong and regulatable promoters Uses of cleavable fusion proteins for affinity purification Cell-free in-vitro translation systems 2 h (Pan SQ) Site-directed mutagenesis Directed molecular evolution 4 h (Pan SQ) Phage display In vitro display technologies Strategies and approaches to enhance biological properties of proteins and enzymes Increasing protein solubility Increasing enzymatic activity, stability and specificity 4 h (Pan SQ) Modifying cofactor requirements 2 h (Song J) Engineering of regulatable enzymes Incorporation of unnatural amino acids 4 h (Song J) Protein and peptide design and engineering Specific examples of protein engineering o Microbial, plant and animal cells as bioreactors 6 h (Pan SQ) o Therapeutic proteins o Industrial enzymes Total Lectures: 26 hrs Tutorials and discussions: 24 hrs Total hours: 50h

2. 3.

4.

5. 6.

TEXT BOOKS (Reference): Protein Engineering: a practical approach / edited by Anthony R. Rees, Michael J.E. Sternberg, and Ronald Wetzel. Oxford ; New York : IRL Press at Oxford University Press , c1992 Protein Engineering and design / edited by Paul R. Carey. San Diego, Calif. : Academic Press , c1996 Protein Engineering: Principles and Practice Jeffrey L. Cleland, Charles S. Craik. John Wiley & Sons. 1996 PRESENTATION/DISCUSSION/TUTORIALS: 24 hours. These will be conducted on the following general topics. Production of proteins and enzymes; Development of improved or novel proteins and enzymes; Industrial proteins and enzymes. MODE OF ASSESSMENT: 50% CA (based on presentations and CA tests), 50% Final Exam (closed-book) MODULE CO-ORDINATOR: A/P Pan Shen Quan LECTURERS: A/P Pan Shen Quan A/P Song Jianxing

(Tel: 6516-2977; E-mail:dbspansq@nus.edu.sg)

(Tel: 6516-2977; E-mail:dbspansq@nus.edu.sg) (Tel: 6516-1013; E-mail:bchsj@nus.edu.sg)

81

SEMESTER I & II LSM4243 TUMOUR BIOLOGY

Prerequisite: LSM2103 Workload: 32 lecture hours + 6 hours (Journal Discussions) + 14 hours (tutorials/student assignments/CAs) This module deals with the understanding of processes that regulate cell growth and proliferation, and the intricate mechanism(s) that result in abnormal proliferation and oncogenesis. Molecular basis of immortalization and the acquisition of the neoplastic phenotype, namely oncogene activation, immune evasion, potential for local and distant spread, and resistance to cell death etc. will be discussed. Role of DNA damage/repair, telomere/telomerase in genome instability and tumourigenesis will be examined. A brief session on target therapies including gene therapy approaches will also be included. Tumour immunology role of inflammation in tumours will be discussed. S/N 1 Topics The Cancer Phenotype: Breakdown of Normal Growth Regulation Steps to immortalisation (Carcinogenesis) Hallmarks of cancer Cell cycles checkpoints Tumour suppressor gene Resistance to death signals Cancer Genetics: Mechanisms of Cell Death Analysis of Apoptosis Genetic Regulation of Apoptosis Apoptosis in Cancer Genome Instability and Cancer DNA damage/repair and mutations Loss of Chromosome integrity Telomeres and Telomerase Molecular Cytogenetics Spread of Cancer Overview of metastatic process Metastatic inefficiency Graft or Host Metastatic suppressor genes Metastatic models Lectures (2 hours each) 4 Shazib Pervaiz

4 Reshma Taneja

4 Prakash Hande

4 Ong Siew Hwa

Total Lectures: 32h Journal Discussion: 6h Tutorials/Student Assignments/CAs: 14h Total hours: TEXT BOOKS (Main References): Selected Research Articles BASIC SUPPLEMENTARY READINGS: Robert A Weinberg 2007 The Biology of Cancer (Garland Science) ISBN: 0815340788 MODE OF ASSESSMENT: Final Exam (55%) (Closed book examination), CA (45%) (Includes reports and assignments) MODULE CO-ORDINATOR: Associate Professor M Prakash Hande LECTURERS: Professor Shazib Pervaiz Associate Professor Reshma Taneja Associate Professor M. Prakash Hande Dr. Ong Siew Hwa 52h

(Tel: 6516-3664, E-mail: phsmph@nus.edu.sg)

(Tel: 6516 6602, E-mail: phssp@nus.edu.sg) (Tel: 65163236, E-mail: phsrt@nus.edu.sg) (Tel: 65163664, E-mail: phsmph@nus.edu.sg) (Tel: 64747210, E-mail: phsosh@nus.edu.sg; siew_hwa_ong@acumen-research.com)
82

SEMESTER I LSM4251 PLANT GROWTH AND DEVELOPMENT

Prerequisite: LSM3253 or LSM3244 Workload: 30 hr lectures and 20 hrs term paper and presentation This module aims to provide an overall view on growth and development of higher vascular plants through their life cycles. Discussion in this module include selected topics in gamete development, fertilization, embryo development, seed germination, development of various plant organs and flowering, the role of plant growth regulators, and the cellular, physiological and molecular basis of plant morphogenesis. The molecular genetic mechanisms of various stages of plant development will be discussed. S/N 1 2 3 Topics Introduction An overview of plant growth and development Microsporogenesis, megasporogenesis and gametogenesis -Anther differentiation -Pollen development and maturation -Male gametogenesis -Pollen germination and growth of the pollen tube -Ovule determination and development -Megasporogenesis Genomic imprinting -Double fertilization -Parent-of-origin effects and allele-specific imprinting -Molecular imprinting mechanisms Epigenetic regulation of plant development Embryo Development - mutants in zygotic embryogenesis Growth and differentiation of the shoot -Vegetative shoot and root apices -Tissue differentiation in the shoot -Leaf growth and development -Development of specialized cells and organs Flowering time control and flower development -Physiological and genetic control of flowering -Floral meristem specification -Flower development Fruit development and ripening -Biochemistry, physiology and molecular biology of fruit growth and ripening -Role of ethylene in fruit development Lecture hours 1 Kumar P, He Y, Yu H 1 He Y 2 He Y

2 He Y

5 6 7

2 He Y 4 Kumar P 6 Kumar P

8 Yu H

4 Yu H

Total Lectures: 30h Assignments and presentations: 20h Total hours: TEXT BOOK: Selected scientific papers MODE OF ASSESSMENT: Final examination: 50%; CA (term paper and presentation): 50% MODULE CO-ORDINATOR: Prof Yu Hao LECTURERS: Prof Prakash Kumar Prof Yu Hao Dr He Yuehui 50h

(Tel: 6516-3048, E-mail: dbsyuhao@nus.edu.sg)

(Tel: 6516-2859, E-mail: dbskumar@nus.edu.sg) (Tel: 6516-3048, E-mail: dbsyuhao@nus.edu.sg) (Tel: 6516-2716, E-mail: dbshy@nus.edu.sg )
83

SEMESTER I LSM4252 ANIMAL REPRODUCTION

Prerequisite: LSM3233 Workload: 30 h lectures + 12 h tutorials/presentations + 8 h assignments/readings This module covers the events and mechanisms leading to the development and differentiation of gonads and sexes in vertebrates, and eventually to the reproduction and propagation of a new generation. It also describes the use of animal models and discusses selected topics to highlight the current trends in animal reproduction. S/N 1. Topics Germ cell biology and reproduction Germ cells in development and in culture Gametogenesis in vitro Reproductive technologies 2. The master regulator of reproduction: the brain Reproductive hormones of the pituitary and their molecular regulation Activation of spermiation and ovulation Modes of fertilization, mammalian implantation and pregnancy 3. The essential tools of (reproductive) genomics Asexual vs. sexual reproduction The war of sexes at various levels The chromosomal basis of sex The molecular basis of sex determination 10 Laszlo Orban 10 Winkler, Christoph W Lecture hours 10 Hong Yunhan

Total Lectures: 30h Tutorials: 6h Student presentations/CAs 6h Assignments & readings 8h Total hours: TEXT BOOK: (1) Essential Reproduction, Martin H. Johnson, Blackwell Publishers, 6th edition (2007). (2) The Genetics and Biology of Sex Determination, (Novartis Foundation Symposium No. 244), D. Chadwick and J. Goode, 2002. John Wiley and Sons. MODE OF ASSESSMENT: 60 % CA (3 x CA 20% each), to be held after each third of the course. CA will be a number of short answer questions. 40 % Final exam (short assays covering the entire course content). MODULE CO-ORDINATOR: A/P Hong Yunhan (Tel: 6516-2915, E-mail: dbshyh@nus.edu.sg) 50h

LECTURERS:
A/P Hong Yunhan A/P Laszlo Orban A/P Winkler Christoph W (Tel: 6516-2915, E-mail: dbshyh@nus.edu.sg) (Tel: 6872-7413, E-mail: laszlo@tll.org.sg) (Tel: 6516-7376, E-mail: dbscwc@nus.edu.sg)

84

SEMESTER II LSM4253 BEHAVIOURAL BIOLOGY

Prerequisite: LSM2251 or LSM3252 Workload: 26 lecture hours + 24 hours tutorial, discussions, group project and/or term paper This module provides an in-depth coverage of the relationships that organisms have with each other and with the environment. Selected key concepts in organismal interactions, illustrated with examples from general diverse animals and ecological systems, to ultimate and proximate explanations of animal interactions and other life history characteristics, will be covered. Students will be given the opportunity to assimilate and critically evaluate contemporary literature on relevant current issues. Experimental group field/lab projects will be designed, proposed and carried out by students to improve their understanding of animal behaviour and to appreciate the significance of behavior in ecology as well as other related disciplines. S/N 1. Topics The study of behaviour: questions about behaviour; ways of thinking about behaviour; formulating and testing hypotheses; understanding proximate and ultimate causes of behaviour Principles of animal behaviour: evolutionary thinking; behaviour and adaptation; Predator-prey interactions: predation and predator avoidance The evolution of foraging behaviour: optimality theory; optimal foraging theory; habitat selection The evolution of communication: signal design; sensory biases; sensory exploitation Sexual selection theory: the evolution of sex; intra-sexual selection; intersexual selection; sexual conflicts; mating strategies, and sperm competition Mating systems and parental care The evolution of social behaviour: costs and benefits of social life; evolution of helping behaviour; the concept of inclusive fitness; the organisation of animal societies Human behaviour: adaptive mating preferences and parental care Animal personality Total Lectures: 26h Tutorials, Discussions, Group Project and/or Term paper: 24h Total hours: TEXT BOOK (Reference book): Alcock, J. 2010. Animal behavior: an evolutionary approach, 9th edition. Sinauer Associate, Inc. MODE OF ASSESSMENT: 60% CA, 40% final exam 50h Lecture hours 26

2. 2. 3. 4. 5. 6. 7.

7. 8.

MODULE CO-ORDINATOR & LECTURER: A/P Li Daiqin (Tel: 6516-4372, E-mail: dbslidq@nus.edu.sg)

85

SEMESTER I LSM4254 PRINCIPLES OF TAXONOMY AND SYSTEMATICS

Prerequisite: LSM1103 Workload: 2 hrs lectures/week + 4hrs/biweekly practicals/tutorials This module introduces students to taxonomy and systematics; i.e., the science of grouping biodiversity into species, describing the species, and classifying this diversity into higher-level taxa that reflect evolutionary history. The module has two main goals: (1) It introduces the main concepts and goals of taxonomy and systematics. (2) It teaches the qualitative and quantitative techniques that are today used to describe/identify species and higher-level taxa based on the analysis of morphological and DNA sequence evidence. The aim is to equip environmental as well as other biologists with a thorough understanding of taxonomic/systematic units and the tools needed for evaluating and quantifying diversity in samples of plant and animal specimens.

S/N 1 2 3 4 5 6

Topics Species Concepts in Theory of Practice Species Identification (Morphology, DNA sequences) Species Richness Estimation Nomenclature: accurate information storage through names Tree-of-Life: Phylogenetic Techniques for Tree Reconstruction Classification and Ranking Total lectures : Tutorials (presentation): Practicals: Total hours:

Lecture hours 6 4 2 2 10 2 26 h 4h 20 h 50h

TEXT BOOK (Reference books): assigned readings from journals MODE OF ASSESSMENT: Final Exam: 50%, Practical and IVLE Participation: 15%, Oral presentation: 15%, Practical Report: 20% MODULE CO-ORDINATOR: Prof. Rudolf Meier LECTURER: Prof. Rudolf Meier (Tel: 6516-2714, E-mail: dbsmr@nus.edu.sg)

86

SEMESTER I LSM4261 MARINE BIOLOGY

Prerequisite: LSM3254 Workload: 26 lecture hours + 24 tutorial hours This module builds on the fundamental material covered in LSM3254. Primary literature and first-hand accounts will be used to explore selected topics, such as specific groups of organisms or particular environments, in more depth. Guest speakers will be asked to present their current work and open discussion will be encouraged. The focus will be on tropical marine biology, with Singaporean examples wherever possible. Students can expect problem-based learning, cooperative learning and small group work.

S/N

Topics Introduction to marine biology An overview of the course structure and content. Recap of differences between the terrestrial and marine ecosystems, basic oceanography, marine ecology, key marine environments, resources from the sea, human impacts and marine environment management. Selected systems and organisms Coral reefs, cetaceans, mangroves, crabs, man-made environments, algae, seagrass beds and giant clams are among the systems and organisms that student can expect to learn more about. Guest speakers will be invited to discuss their specialties. Talks may cover groups such as macro-algae, zoanthids, phyto-plankton, barnacles, polychaetes, selected fish taxa, gastropods, etc. Human-ocean interactions Living (renewable) and non-living (non-renewable) resources and their rates and patterns of exploitation. Impacts of human activities, both localized and global. The state of the marine environment, management of endangered species and critical habitats, management systems relevant to marine ecosystems including restoration techniques.

Lecture/ tutorial hours 8

1.

2.

22

3.

20

Total Lectures: 26h Tutorials: 24h Total hours: REFERENCE BOOKS: Key text: Castro, P. & Huber, M. Marine Biology. McGraw Hill. Sumich, J. An Introduction to the Biology of Marine Life. McGraw-Hill. Milne, D. Marine Life and the Sea. Wadsworth. Little, C. & Kitching, J.A. The Biology of Rocky Shores. Oxford University Press. Raffaelli, D. & Hawkins, S. Intertidal Ecology. Oxford: Chapman and Hall. MODE OF ASSESSMENT: Continual assessment 60%; final open-book exam 40%. MODULE CO-ORDINATOR: Dr Peter Todd (Tel: 6516-1034, E-mail: dbspat@nus.edu.sg) LECTURER: Dr Peter Todd 50h

(Tel: 6516-1034, E-mail: dbspat@nus.edu.sg)

87

SEMESTER II LSM4262 TROPICAL CONSERVATION BIOLOGY

Prerequisite: LSM2251 Workload: 26 lecture hours + 24 tutorial/Discussions hours Conservation and the loss of biodiversity and natural ecosystems are currently regarded as one of the most pressing problems facing mankind. The course will highlight the impact of habitat loss on biodiversity and the basis for formulation of effective conservation management strategies. The course will also introduce students to the theory of current conservation biology as illustrated by applications in tropical areas, species conservation issues, ecological challenges, role of zoological gardens, legal challenges etc. Conservation of tropical biota, management of local and regional environmental problems, appreciation and consideration of the socio-economic issues will also be treated. Conservation priorities and developmental needs at the national level will also be discussed, with emphasis on Singapore and SE Asia. The course will have guest lecturers from overseas as well as managers and conservation-players from the local environment. It will also involve a special round-table discussion on specific conservation issues. S/N 1. Topics Introduction Species Loss, case studies Threatened and endangered species with case studies Timber trade, pet trade and wildlife usage Ex-situ versus in-situ conservation International guidelines Ecological theory of conservation The problems of alien species Conservation challenges in SE Asia Perspectives in regional and local conservation Conservation options and challenges Invited Lecture/Discussion 1: Implementing Conservation policies in Singapore (with a discussion of national priorities) Invited Lecture 2: Invited Speaker Lectures/Practicals/ Discussions 14 h N.K Ng

2.

12 h M.R.C. Posa

4.

4h National Parks Board 4h To be determined

5.

6.

Invited Lecture/Discussion 3: Challenges in marine conservation in Singapore and the region. Invited Lecture/Discussion 4: The role of NGOs in national conservation (with a discussion)

4h To be determined 4h President, Nature Society of Singapore 4h Professor Lye Lin Heng (APCEL, Faculty of Law) 4h Mr. N. Sivasothi Total Lectures: 26h Tutorials/Discussions: 24h Total hours: 50h

7.

8.

Invited Lecture 6: Legal issues with the CBD and biodiversity challenges (with a discussion)

9.

Invited Lecture/Discussion 7: Conservation lessons from Chek Jawa

REFERRENCE BOOKS: Chapman & Hall; Soule, M. E. (ed.), 1986. Conservation biology. The Science of Scarcity and Diversity. Sinauer Associates Inc. Publishers. Davision, G.W.H., P.K.L. Ng & H.C. Ho, 2008. The Singapore Red Data Book. Nature Society Singapore. Francesch-Huidobro, M., 2008. Governance, Politics and Environment. A Singapore Study. Institute of Southeast Asian Studies.
88

Groombridge, B. (ed.), 1992. Global Biodiversity. Status of the Earth's Living Resources. A report compiled by the World Conservation Moniering Centre. Literature from journals, academic reviews, current science magazines etc.. Sodhi , N.S. , Acciaioli, G, M. Erb, & A. K.-J.. Tan, (eds), 2008. Biodiversity and human livelihoods in protected areas: case studies from the Malay Archipelago. Cambridge University Press. Sodhi, N.S. & Brook, B.W., 2006. Southeast Asian biodiversity in crisis. Cambridge University Press. Sodhi, N.S., B.W. Brook & C.J.A. Bradshaw, 2007. Tropical conservation Biology. Wiley-Blackwell. Wilson, E.O., 1988. Biodiversity. National Academy Press, Washington D.C. etc..

TUTORIALS: The tutorial sessions will be integrated into the several guest lectures which will all be followed by 1-2 hours of discussions of the topics at hand. These discussions will examine these areas in depth and dwell on the biological, political, economic and sociological forces at work. Lecturers and TAs will act as resource personnel and help direct the discussions. MODE OF ASSESSMENT: 40% CA; 60% semestral examination. There will be a formal written examination (essay and short questions) that will constitute 60% of the total. The other 40% will be from one semester-long written assignment based on actual scenarios, and students (working in teams) will then have to defend these ideas in a final round-table discussion at the end of the semester. Some of the topics can be on the challenges and constraints posed to scientists by the regulations and needs of the international Convention on Biodiversity, and how it has helped and/or impeded biodiversity research or the role of education on conservation. MODULE CO-ORDINATOR: Dr Ng Ngan Kee (NNK) LECTURERS: Dr. Mary Rose Cervantes Posa (Mingko) Dr Ng Ngan Kee (NNK)

(Tel: 6601 1091; E-mail: dbsngnk@nus.edu.sg)

(Tel: 6516 1285; E-mail: dbspmrc@nus.edu.sg) (Tel: 6601 1091; E-mail: dbsngnk@nus.edu.sg)

89

SPECIAL SEMESTER, TERM IV LSM4263 FIELD STUDIES IN BIODIVERSITY

Prerequisite: For Life Sciences Honours students or by departmental approval Workload: 12 lecture hours + 8 field practical hours + 7 day field course LSM4263 will introduce students to field biology, conservation issues, the basic techniques involved, sampling design and basic data gathering and data management. From field practicals, students will experience and encounter tropical environs and habitats, namely coastal, mangrove, primary and secondary forest. A 7 day field course is incorporated and will be conducted in Pulau Tioman, Malaysia. There students, who will be divided into small groups, will conduct either 4 mini-projects in 4 separate habitats or one major project in a chosen habitat, under the supervision of experienced field-orientated teaching assistants. This module will involve overseas university students as well as NUS Life Science students. S/N 1. 2. 3. 4. 5. 6. 7. 8. 9. Topics Introduction to field biology and Pulau Tioman Terrestrial Biomes Aquatic ecosystems Confessions of a Field Biologist Sampling design, data analysis and report writing Diversity of Pulau Tioman flora and fauna Sungei Buloh Wetland Reserve field practical Bukit Timah Nature Reserve field practical Field course conducted at Pulau Tioman Lecture hours 2 2 2 2 2 2 4 4 7 days Total Lectures: 12h Practicals: 2x4 = 8h Total hours 20 + 7 days

REFERENCES: Chek Jawa guide book, BP mangrove series, BP seashore guide, BP aquatic life guide, Raffles Bulletin of Zoology supplement 6 (Pulau Tioman biodiversity, available in pdf format from http://rmbr.nus.edu.sg/rbz/biblio/#s6); Natural History of Tioman available from Science Library. MODE OF ASSESSMENT: MODULE CO-ORDINATOR: Dr Tan Heok Hui LECTURERS: Asst. Prof Peter Todd oral presentation, peer review, report assignments (only for local students)

(Tel: 6516-1662, E-mail: dbsthh@nus.edu.sg)

(Tel: 6516-1034, E-mail: dbspat@nus.edu.sg)

90

SEMESTER II LSM4264 FRESHWATER BIOLOGY

Prerequisite: LSM3254 Workload: 26 lecture hours + 24 tutorial/discussion/field trip hours Freshwater is essential to life, yet constitutes less than 3% of Earths total water. With many freshwater ecosystems under threat, understanding the biology of freshwaters is fundamentally important to their management, conservation, and restoration. This module introduces the study of inland waters. Through lectures, directed readings and discussions, field trips, and project work, we will focus on topical areas including aquatic biodiversity and ecology, aquatic conservation, freshwater ecosystem services, threats to freshwaters, anthropogenic effects, sustainable water use, aquatic invasive species, and policies, regulation and management of freshwater resources in local and international contexts. S/N 1. 2. 3. 4. 5. 6. Topics Lecture hours 26

7.

8.

Introduction: Course overview; introduction to limnology Freshwater habitats: Types/morphologies; thermal characteristics and water movement Aquatic organisms: Diversity and ecology (e.g., species interactions in community ecology); sampling techniques Freshwater ecosystems: Primary productivity; trophic dynamics; chemical/nutrient cycling Aquatic conservation: Conceptual framework; freshwater ecosystem goods and services Human water use: Domestic, agricultural, and industrial; policies, regulation, and management; sustainable harvesting (e.g., water, fisheries); habitat modification (e.g., dams and reservoirs); pollution and climate change issues (e.g., eutrophication, acidification) Aquatic invasive species: Introduction to invasion biology and aquatic invasive species; invasion process and pathways; prevention, detection, and management of aquatic invasive species; invasive species policies and issues (e.g., ballast water) International water issues and policies: Case studies of regional and international management and agreements; future scenarios Total lectures: 26h Tutorials/discussions/field trips: 24h Total hours: 50h

REFERENCES: Dodson, S., 2004. Introduction to Limnology. McGraw-Hill. Dodds, W. K., 2002. Freshwater Ecology: Concepts and Environmental Applications. Academic Press. Dudgeon, D. (ed.), 2008. Tropical Stream Ecology. Academic Press. Yeo, D. C. J,, Wang, L. K,, Lim, K. K. P., 2010. Private Lives: An Expos of Singapores Freshwaters. Raffles Museum of Biodiversity Research. Yeo, D. C. J., Lim, K. K. P., 2011. Freshwater Ecosystems. In: Ng, P. K. L., Corlett, R. T., Tan, H. T. W., (eds.) Singapore Biodiversity: An Encyclopedia of the Natural Environment and Sustainable Development. Raffles Museum of Biodiversity Research. Pp. 5263. Selected scientific papers and technical reports. MODE OF ASSESSMENT: Continual assessment 60%; final open-book exam 40%. MODULE CO-ORDINATOR/LECTURER: Dr Darren Yeo (Tel: 6516-2709, E-mail: dbsyeod@nus.edu.sg)

91

SEMESTER II LSM4265 URBAN ECOLOGY

Prerequisite: LSM2251 and LSM3255 Workload: 26 lecture hours + 24 practical hours This module introduces students to the ecology of urban areas, with a focus on tropical cities. It will deal with terrestrial, freshwater and coastal marine environments in which urbanization is the key ecological factor. Topics covered will include the origins of cities, urbanization as a process, urban landscapes, urban environments (soils, hydrology, climates and pollution), urban biodiversity, alien species, landscape design, urban greenery, pest and vector control, ecological footprints, and the sustainable city. Students will undertake a small-group research project involving the design, implementation, analysis and presentation of an urban ecology study.

S/N 1 2 3

4 5

6 7 8 9

10 11 12

Topics Urbanization as a process: definitions; the first cities; tropical cities; urbanization in the 21st century. Urban ecosystems: cities as ecosystems; abiotic, biotic and social factors; flows of energy and materials. Urban environments: urbanization and soils; anthrosols and technosols; urban hydrology impervious surfaces, rainfall, drainage, evaporation, flooding, aquifers; urban climates; the heat island effect and its amelioration Urban pollution: sources, movements, and mitigation; air pollution; water pollution; noise. Urban biodiversity: exploiters, adapters and avoiders; natives vs. aliens; spontaneous and managed components; the role of remnant natural habitats; pest species. Urban vegetation: key features of urban vegetation; the tropical urban savanna; urban vegetation as animal habitat Urban animals: urban birds as a model system; urban mammals, insects, other invertebrates. Urban freshwater: natural and semi-natural ecosystems; rivers, canals, lakes, reservoirs, ponds and drains; urban freshwater wetlands. Urban coastlines: harbours; seawalls; coastal waters; beaches; mangroves; coral reefs (urban reefs); shipping activities, pollution, key management issues; coastal restoration. Cities for people: population densities; urban design; landscape management; public health issues. Urban greening: trees in parks and along roadsides; urban arboriculture; management of turf; turfgrass ecosystems; flowerbeds. The ecological city: myths and realities. Balancing the natural and built environments; minimizing footprints; cities of the future. Total lectures : Field trip and group projects: Total hours:

Lecture hours 2 2 4

2 2

2 2 2 2

2 2 2 26h 24h 50h

TEXT BOOK (Reference books): a reading list will be provided. MODE OF ASSESSMENT: 50% continuous assessment (project reports and presentation), 50% closed-book exam. MODULE CO-ORDINATOR: Prof R.T. Corlett LECTURER: Prof R.T. Corlett and guest lecturers (Tel: 6516-1285; E-mail: dbscrt@nus.edu.sg)

92

General information concerning Life Sciences Teaching Laboratories

Labs
LSLab1

Location
DBS S1A#0323

Nature
WetLab

Lab Manager
LiewChyeFong(DBS)

Lab Officers
WooHinCheow(DBS) OhSeahLiew(DBS)

Student capacity
120 students

Major equipment
Fractioncollectors UVspecs Waterbaths Peristalticpumps Microscopes LinkedVideoSystem HPLC CE Microtiterplatereader Refrigeratedbenchtop centrifuges Balances Balances Osmometers Circulators Centrifuges Microscopes Spectrophotometers
Real time PCRs Ovens Centrifuges Agarose gel sets Power packs SDS-PAGE gel sets Micropipettes,Freezers UV-specs Water baths Gel documenttion system Nanodrop Incubators AV System, Fluorescent Inverted Microscopes, micro-injectors; Gene gun and PDS, sonicator Thermo-mixers, Chiller, Heat blocks, pH meters, Safety hoods, CO2 Incubators, Speed vac, Microplate reader

Potential LS modules
LSM1102,LSM1401 LSM2201A,LSM3262

LSLab2

DBS S1A#0305

WetLab

AngSweeEng (DBS)

TayBeeLing(DBS) EricaXu(DBS)

80students

LSM1102,LSM1104, LSM1401,LSM3233, LSM3262,FST2106

LS Lab - 3

DBS S1A# 04-21

Wet Lab

N.Subha (DBS)

Yan Tie (DBS) Allan Tan (DBS)

120 students

LSM1103, LSM2202A, LSM3233, LSM3244, GEK1542 (Forensic Science)

93

General information concerning Life Sciences Teaching Laboratories

Labs
LSLab4

Location
DBS S1A#0403

Nature
WetLab

Lab Manager
ChuaLingLih (DBS)

Lab Officers
TanLuWee(DBS) FoongChoyMei (DBS)

Student capacity
80 students

Major equipment
Centrifuges Agarosegelsets Powerpacks Micropipettes UVspecs Waterbaths Microscopes Laminarflows Incubators Geldocsystem Computers

Potential LS modules
LSM1102,LSM1301, LSM3233,LSM3244, SP3202 *(OpenLabforspillover courses)

LSLab5

DBS SIA#0406

DryLab

HoChongMun (DBS)

YapWP(DBS)

36/15 (51) students

LSLab6

DBS S1A#0402

DryLab

WongWai Peng(DBS)

XuLiyuan(DBS) Salim(DBS) ReubenYue(DBS) GohPohMoi(DBS) Morgany(DBS)

25 students/ session 180 students

Biopacdataacquisition units

LSLab7

DBS S2#0304

DryLab

TommyTan (DBS)

Microscopes Wet/Dryspecimens

LSM3241 Projectwork; HandsonWorkshops; OnlineCDROMfor GEK1527/LSM1302; LSM4254 LSM1201,LSM1302, LSM3262(presentation/ tutorial)(Physiology), GEK1542 LSM1103,LSM1104, LSM1303,LSM2251, LSM3252,LSM3254, LSM3255,LSM3261, LSM3256,LSM3272, LSM4254,LSM4265 *(OpenLabforallBioD modules)

94

General information concerning Life Sciences Teaching Laboratories

Labs
LSLab8 (Biochemistry)

Location
MD7 Level4

Nature
Bothwet anddry Lab

Lab Manager
OoiSuanOo (Biochemistry)

Lab Officers
ShaLanjie (Biochemistry) QianFeng (Biochemistry)

Student capacity
200 students

Major equipment

Potential LS modules

LSM1101 Waterbaths,Microfuges, LSM2201A Refrigeratedbench LSM2202A centrifuges,Incubators, LSM3231 Ovens,Balances,PCR, LSM3224 GelDocsystem,Freezers, InvertedMicroscope, Thermomixers,Chiller, LSLab8isalsousedas: Heatblocks,pHmeters, 1. LSMCA&tutorial venues Biohazardsafetyhood, 2. Examcentre CO2Incubator,Speed vac,LinkedVideo System,HPLC,CE, autoclaves, Spectrophotometers, DNAsequencer,Real TimePCR,microplate reader,nitrogen evaporator,TLC autospotter,nanodrop spectrometer

95

General information concerning Life Sciences Teaching Laboratories

Labs
LSLab9 (Microbiology)

Location
MD4, Level4

Nature
WetLab

Lab Manager
LimEkWang (Microbiology)

Lab Officers
SasikumarRajendran, ChanYueNg

Student capacity
200 students

Major equipment
PowerPack,MiniGelTanks, Micropipettes,Microfuge, GelDocSystems,UVVis Spectrophotometers, Microscopes,SpeedVac Concentrator,Heating blocks,HybridOvens, ShakerIncubators,Bench topRefrigerated Centrifuges, AnaerobicChamber, Fluormeters,Riboprinter, Freezers,Chillers, Autoclaves,ElisaPlate Reader,Flowcytometer, DNAsequencer,BacteriaID System,SpiralPlater, Incubators,CO2incubators, Elispotbioreader,Biohazard SafetyCabinets,Lab.Flow Cabinet,Waterbaths, Fermentors,Rotary Evaporators,PhastSystem, FrenchPress,Ovens, FreezeDryer, Ultrasonicator,Protein Concentrator,Pumps, Vortexmixers, Luminometers,Hotplate Stirrers,Thermomixer,5 HeadTeachingMicroscope.

Potential LS modules
LSM1102 LSM1103 LSM2202 LSM3223 LSM3225 LSM3232 LSM3242 LSM4223

96

Minimal Safety Requirements for all Teaching Laboratories

Familiarise yourself with any additional rules relating to the laboratory in which you are working. Note the location of eye wash points, safety shower, fire extinguisherandfirstaidcabinets. Washyourhandsthoroughlywithsoapandwaterbeforeleavingthelaboratory. Neverpipetteanythingbymouth. Reportanyaccidenttothedemonstratorsimmediately. Handlesharpsandsyringeswithextremecaution. Ifyouhaveanabrasionorcutonyourhands,coveritbeforeyoubeginwork. Neverleavealightedburnerunattended. Neveruseanopenflameinthevicinityofflammablesolvents. Do not eat, drink or apply cosmetics in the laboratory (especially nail polish flammablesolvents) Longhairshouldbetiedback. Suitable footwear mustbe worn to protect against spills; do not wear sandals or slippers. Smokingisstrictlyprohibited. Laboratory coats must be worn at all times in the laboratory, but removed beforeleavingthelaboratory. Gogglesmustbewornwhenhandlingdangerouschemicals.

97

List of Module Coordinators (Levels 1 4) Module Code LSM1101 Module Title Biochemistryof Biomolecules MolecularGenetics MCs 4 Semester 1and2 Coordinators DengLihWen(S1) TooHengPhon(S2) ChewFookTim

LSM1102

1and2

LSM1103

Biodiversity

1and2

N.Sivasothi(S1); NgNganKee(S2) IpYuenKwong

LSM1104

GeneralPhysiology

1and2

LSM1202

HumanAnatomy

HeBeiping

LSM1301

GeneralBiology

1,2and4

SeowTeckKeong(S1); LohChiangShiong(S2); WuJinlu(S4) LamSiewHong(S1&S4); K.Swaminathan(S2) N.Sivasothi

LSM1302/ GEK1527 LSM1303

GenesandSociety

1,2and4

AnimalBehaviour

LSM1401

Fundamentalsof Biochemistry ChemistryforLifeSciences

1and2

KimChuYoung(S1); SeowTeckKeong(S2) LuYixin; TanSueQingEmelyn TeoYikYing

CM1401

ST1232

StatisticsforLifeSciences

1and2

98

 Module Code LSM2101 Module Title MetabolismandRegulation MCs 4 Semester 1and2 Coordinators TheresaTanMayChin(S1); GaneshSAnand(S2) DingJeakLing

LSM2102

MolecularBiology

1and2

LSM2103

CellBiology

1and2

ChangChanFong(S1); YeongFoongMay(S2) RManjunathaKini(S1); MaxeyChung(S2) WangShu(S1); KJeyaseelan(S2); NorbertLehming(S2) LeeYuanKun(S1); NgMahLee(S1); SimTiowSuan(S2); JustinChu(S2) TanTinWee

LSM2201A

ExperimentalBiochemistry

1and2

LSM2202A

ExperimentalMolecularand CellBiology ExperimentalMicrobiology

1and2

LSM2203

1and2

LSM2241

IntroductoryBioinformatics

1and2

LSM2251

EcologyandEnvironment

1and2

N.Sivasothi

LSM2288/9

UROPS

4or8

1,2and Special Semester

LiouYihCherng (S1&S2) WuJinlu(SpecialSemester)

99

 Module Code LSM3211

Module Title FundamentalPharmacology

MCs 4

Semester 1and2

Coordinators FredWong LimHsiuKim,Lina

LSM3212

HumanPhysiology: CardiopulmonarySystem

1and2

LSM3213

MolecularandCellular Neurobiology

SoongTuckWah

LSM3214

HumanPhysiology: HormonesandHealth

WongChongThim

LSM3221

HumanPharmacology

GavinDawe

LSM3223

Immunology

1and2

WongSiewHeng(S1); PaulMacAry(S2)

LSM3224

MolecularBasisofHuman Diseases

MridulaSharma

LSM3225

MolecularMicrobiologyin HumanDiseases

JustinChu TanYeeJoo

LSM3231

ProteinStructureand Function

MaxeyChung

LSM3232

Microbiology

1and2

SimTiowSuan

100

Module Code LSM3233

Module Title DevelopmentalBiology

MCs 4

Semester 1

Coordinators Winkler,ChristophWolfram

LSM3241

Bioinformaticsand Biocomputing

TanTinWee

LSM3242

AppliedMicrobiology

LeeYuanKun

LSM3243

MolecularBiophysics

YangDaiwen

LSM3244

MolecularBiotechnology

1and2

JayaramanSivaraman(S1); GongZhiyuan(S2)

LSM3252

EvolutionandComparative Genomics

NgNganKee

LSM3254

EcologyofAquatic Environments

PeterAlanTodd

LSM3255

EcologyofTerrestrial Environments

EdwardWebb

LSM3256

TropicalHorticulture

HughTan

101

 Module Code LSM3261

Module Title LifeForm&Function

MCs 4

Semester 1

Coordinators HughTan

LSM3262

EnvironmentalAnimal Physiology

IpYuenKwong

LSM3263

FieldStudiesinNeotropical Ecosystems

ZeehanJaafar

LSM3272

GlobalChangeBiology

ZeehanJaafar

LSM3288/9

UROPs

4and8

1,2and Special Semester

LiouYihCherng (S1&S2); WuJinlu (SpecialSemester)

102

 Module Code LSM4199 Module Title ResearchProject MCs 16 Semester 1and2 Coordinators DingJeakLing

LSM4211 LSM4212

Toxicology PharmacogeneticsandDrug Responses SystemNeurobiology

4 4

1 1

ShenHanMing EdmundLee

LSM4213

WongBoonSeng

LSM4214

CancerPharmacology

JohnLuk

LSM4221

DrugsDiscoveryandClinical Trials AdvancedImmunology

TanChayHoon

LSM4222

1and2

VeroniqueAngeli(S1) GanYunnHwen(S2) KevinTanShyongWei, VincentChowTakKwong BarryHalliwell

LSM4223

AdvancesinAntimicrobial Strategies FreeRadicalsand AntioxidantBiology GeneticMedicineinthe PostGenomicEra InfectionandImmunity

LSM4224

LSM4225

CarolineLee SamuelChong SylvieAlonso VeroniqueAngeli YangDaiwen

LSM4226

LSM4231

StructuralBiology

LSM4232

AdvancedCellBiology

ManoorPrakashHande

LSM4241

FunctionalGenomics

MaxeyChung

103

Module Code LSM4242

Module Title ProteinEngineering

MCs 4

Semester 1

Coordinators PanShenQuan

LSM4243

TumourBiology

1and2

ManoorPrakashHande

LSM4251

PlantGrowthand Development AnimalReproduction

YuHao

LSM4252

HongYunhan

LSM4253

BehaviouralBiology

LiDaiqin

LSM4254

PrinciplesofTaxonomyand Systematics MarineBiology

RudolfMeier

LSM4261

PeterTodd

LSM4262

TropicalConservation Biology FieldStudiesInBiodiversity

NgNganKee

LSM4263

TanHeokHui

LSM4264

FreshwaterBiology

DarrenYeo

LSM4265

UrbanEcology

RichardCorlett

104

LIFE SCIENCES ACADEMIC ADVISORS FOR LEVELS 1,2,3 & 4 Period when Advisor should be on campus

Department
Biochemistry BiologicalSciences Microbiology Pharmacology Physiology

Name of Advisor
A/PTooHengPhon A/PChangChanFong ProfJeyaseelan A/PMaxeyChung DrPeterAlanTodd DrSeowTeckKeong ProfKiniManjunatha DrOngBeeLian A/PGeRuowen A/P HenryMok (HonoursProject) ProfDingJeakLing (HonoursProject) A/PLeeYuanKun Dr.NorbertLehming A/PSimTiowSuan A/PLeeYuanKun A/PFredWong ProfPeterWong A/PJohnLuk A/PWongChongThim A/PHerbertSchwarz

Level
1000 2000 3000 4000 1000 1000 2000 3000 3000 4000 4000 1000 2000 3000 4000 3000 4000 4000 3000 4000

Contacts
65163687 65163681 65163248 65163252 65161034 65162695 65165235 65162852 65167879 65162967 65162776 65163284 65163499 65163280 65163284 65163263 65163266 65164516 65163232 65167773

Email
bchtoohp@nus.edu.sg bchccf@nus.edu.sg bchjeya@nus.edu.sg bchcm@nus.edu.sg dbspat@nus.edu.sg dbsstk@nus.edu.sg dbskinim@nus.edu.sg dbsongbl@nus.edu.sg dbsgerw@nus.edu.sg dbsmokh@nus.edu.sg dbsdjl@nus.edu.sg micleeyk@nus.edu.sg micln@nus.edu.sg micsimts@nus.edu.sg micleeyk@nus.edu.sg phcwongf@nus.edu.sg phcwth@nus.edu.sg phcljm@nus.edu.sg phswct@nus.edu.sg phssh@nus.edu.sg

Onlineregistration tobeginduringthe 3rdweekofJuly. Pleasebeavailable fromthemiddleof Julytoanswer studentqueries

105

 Life Sciences Undergraduate Programme Committee ProfPaulMatsudaira A/ProfTanTinWee A/ProfRogerTan A/ProfMaxeyChung A/ProfChewFookTim A/ProfLeeYuanKun ProfMichaelKemeny 65162692 65163242 65166303 65163252 65161685 65163284 65163307 dbshead@nus.edu.sg bchhead@nus.edu.sg scitance@nus.edu.sg bchcm@nus.edu.sg dbscft@nus.edu.sg micleeyk@nus.edu.sg michead@nus.edu.sg

Coordinators of SubCommittees Undergraduate Research Opportunities (UROPS) & Independent Study Modules (ISM) DrLiouYihCherng(Chair) DrNorbertLehming(Microbiology) A/ProfTangBorLuen(Biochemistry) DrYewWenShan(Biochemistry) A/ProfWongChongThim(Physiology) A/ProfGavinDawe(Pharmacology) A/ProfGeorgeYip(Anatomy) DrWuJinlu(BiologicalSciencesSpecialTerm) Student Exchange Programme (SEP) DrOngBeeLian(IncomingandOutgoing) Curriculum A/ProfChewFookTim A/ProfMaxeyChung Filing for Graduation DrOngBeeLian(IncomingandOutgoing) Support Staff for All Committees Dept. of Anatomy VioletTeo Dept. of Biochemistry FatinUmairah LimSookMinAmanda Dept. of Biological Sciences JacquelineLim LimMiahKyan SallySoh Dept. of Chemistry CarrieWong EmaizaBinteMohdArif Dept. of Microbiology SitiMaryamBinteMasnor 65162852 dbsongbl@nus.edu.sg 65167711 65163499 65161040 65168624 65163232 65168864 65163206 65168476 dbslyc@nus.edu.sg micln@nus.edu.sg bchtbl@nus.edu.sg bchyws@nus.edu.sg phswct@nus.edu.sg phcdgs@nus.edu.sg antyipg@nus.edu.sg dbswjl@nus.edu.sg

65162852

dbsongbl@nus.edu.sg

65161685 65163252

dbscft@nus.edu.sg bchcm@nus.edu.sg

65163204

antteov@nus.edu.sg

65163240 65163240

bchfuma@nus.edu.sg bchlsma@nus.edu.sg

65162703 65162698 65162788

dbsjlsy@nus.edu.sg dbslmk@nus.edu.sg dbssohse@nus.edu.sg

65166361 65162922

chmwst@nus.edu.sg chmema@nus.edu.sg

65167981

micsmm@nus.edu.sg

106

Dept. of Pharmacology HaryatiBteHafid TanBeeChing Dept. of Physiology MichelleChanBixiu KamsitahBinteBoreng Dept. of Statistics and Applied Probability ThongSiokKay,Melissa

65163265 65163264

phchh@nus.edu.sg phctbc@nus.edu.sg

65164890 65164890

phscbm@nus.edu.sg phskb@nus.edu.sg

65168050

statskm@nus.edu.sg

107