Research Project in Biotechnology (D22M01 MY)

Laboratory Risk Assessment

By student ID 009921
School of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga, 43500, Semenyih, Selangor.

Faculty of Science Risk Assessment Form (Version 1)

Name of Assessor: 00921 Research Area: Microbiology (for Research Project in Biotechnology) Experiment Location: Microbiology lab (C1B01) and Bioscience Lab (CB31)

Date of Assessment:

Date for Re-Assessment:

Protocol Title: Search of bacteriostatic properties of a Moraceae hybrid species, Artocarpus heterophyllus x integer Brief Description: Respective agar and liquid media are prepared to grow the selected bacteria to be tested with the extracts (hexane, ethyl acetate and ethanol) of Moraceae hybrid species. Bacteria will be grow in liquid broth to determine their growth curve. The bacteria is let to grow until the exponential phase and used for pour plate diffusion method and turbidometry method. Different concentration of extracts is inoculated into the sterile discs for pour plate method and pipetted into the liquid media for turbidometry method to determine the minimum inhibitory concentration (MIC) and survival index (SI). Bacteria that shows the most susceptibility against the extracts are chosen to observe the morphological changes under scanning electron microscopy. Hazard Assessment Hazard Severity L/M/H Precaution Risk with precautions

Heat injuries Bunsen burners Flaming bottles Water bath and molten agar Autoclave machine M M M Use heatproof gloves if necessary. H Always handled by the technician. L Handle with care, switch off after use. Allowed bottles and loops to cool before use. L L L

Electrical shock Water bath, fridge L Handle with care, avoid operation using wet fingers. Always handled by the technician. Always handled with supervision. L

Autoclave machine Scanning electron Microscopy (SEM) Turbidometric

L L

L L

Operational training until competence.

Faculty of Science 23 July 2010

Faculty of Science Risk Assessment Form (Version 1)

Bacterial culture M and inoculated media might harm human health due by ingestion due cross contamination or through aerosols can harm human health. Use of Group I and Group II microbes. (Staphylococcus aureus,S. epidermidis, Bacillus cereus, Citrobacter freundii, Escherichia coli, Salmonella gallinarium, S. enteriditis and S. arizonae) M

Use biosafety cabinet class II (BSC II).

Wear proper personal protective equipment L (PPE) including lab coat, goggles and gloves. Cover up any open wounds, cuts or burns using plasters or suitable dressings. Wash hand with soaps after experiments and on leaving lab. Clean the workplace with 70% alcohol (for Class I microbes) and 1% virkon (for Class II microbes) regularly including before and after the experiment. Not to bring culture and contaminated equipment to the dining area and do not bring food and drinks to the lab area. Not to perform the experiment with other experiment in the same area of workspace. L

1% Virkon may cause irritation to eyes, respiratory system and skin

Disposal Procedures

Wear nitrile gloves and goggles.

Chemical / Buffer / Reagent

Disposal Route

Contaminated bottles, discs, culture of bacteria on agar plates and liquid media.

Contaminated products and bacterial culture are gathered into blue biohazard bag to be autoclaved by the technician and disposed through a specialist waste company. The contaminated bottles are soaked in container containing 1% Virkon at least 30 minutes and cleaned followed by autoclave procedure by the technician.

Used pipette tip

Used pipette tip is disposed in a jar containing 1% Virkon for at least 30 minutes, then technician to proceed to autoclave procedure.

Level of Supervision: Need training/supervision from technician or competent user for

handling the Varioskan turbidometry machine until competence, always use scanning electron microscope (SEM) under supervision and always follow safety procedures.

Faculty of Science 23 July 2010

Faculty of Science Risk Assessment Form (Version 1)

Notes: Always work within office hour. Signed by Assessor: Name in full: 009921 Signed by Reviewer: Name in full: DR SANDY LOH HWEI SAN

Faculty of Science 23 July 2010