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EXPERIMENTAL METHOD
E x p e r i m e n t s w e r e c a r r i e d out on 17 r a b b i t s weighing 2.5-3 kg. Group 1 consisted of control a n i m a l s
(9 rabbits), group 2 of animals r e c e i v i n g c h o l e s t e r o l in a dose of 0.5 g / k g body weight daily with the diet
for 8 months (8 r a b b i t s ) . The m o s t m a r k e d changes in a t h e r o s c l e r o s i s a r e o b s e r v e d in the f e m o r a l v e s s e l s ,
at the bifurcation of the a o r t a , and in the abdominal a o r t a . Accordingly, e l e c t r i c a l activity was studied in
the abdominal p a r t s of the a o r t a and i n f e r i o r vena cava, and also in the f e m o r a l a r t e r y and vein. F o r this
p u r p o s e , the blood v e s s e l to be investigated was excised under intravenous u r e t h a n e a n e s t h e s i a (10 m l of a
20% solution) and all the p e r i v a s c u l a r t i s s u e s c a r e f u l l y r e m o v e d . The d i s s e c t e d p a r t of the blood v e s s e l ,
4-5 cm long, was incised longitudinally. The a p p a r a t u s used to study e l e c t r i c a l activity of the v e s s e l wall
in vitro is s h o w n d i a g r a m m a t i c a l l y in Fig. 1. The segment of blood v e s s e l f o r testing was placed between
two halves of a t r a n s p a r e n t p l a s t i c c h a m b e r , joined t o g e t h e r h e r m e t i c a l l y by m e a n s of s c r e w s . The two
halves of the c h a m b e r w e r e filled with K r e b s ' solution of the following composition (in g / l i t e r ) : NaCl 7.7;
NaHCO 3 1.36; NaH2FO 4 0.165; KC1 0.347; CaC12.6H20 0.272; MgC12.6H20 0.01; glucose 1.4. The K r e b s '
solution was t i t r a t e d to pH 7.4. A g a r bridge e l e c t r o d e s , p r e p a r e d in the usual way, w e r e placed 2 m m away
f r o m the s u r f a c e of the intima and adventitia of the blood v e s s e l . The o t h e r ends of the a g a r b r i d g e e l e c -
9 1971 Consultants Bureau,. a division of Plenum Publishing Corporation, 227 West i7th Street, New York,
N. Y. 10011. All rights reserved. This article cannot be reproduced for any purpose whatsoever without
permission of the publisher. A cop)" of this article is available from the publisher for $t5.00.
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