TGF beta 1 protein (ab50036)

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Product overview
Protein description Recombinant human, (mature) protein expressed in mammalian A293 cells. This recombinant protein is a 25.0 kDa protein with
each subunit containing (C terminal) 112 amino acid residues, linked by a single disulfide bond.
Protein info
Function Multifunctional protein that controls proliferation, differentiation and other functions in many cell types. Many cells synthesize
TGFB1 and have specific receptors for it. t positively and negatively regulates many other growth factors. t plays an important role
in bone remodeling as it is a potent stimulator of osteoblastic bone formation, causing chemotaxis, proliferation and differentiation
in committed osteoblasts.
Tissue specificity Highly expressed in bone. Abundantly expressed in articular cartilage and chondrocytes and is increased in osteoarthritis (OA).
Co-localizes with ASPN in chondrocytes within OA lesions of articular cartilage.
nvolvement in disease Defects in TGFB1 are the cause of Camurati-Engelmann disease (CE) [MM:131300]; also known as progressive diaphyseal
dysplasia 1 (DPD1). CE is an autosomal dominant disorder characterized by hyperostosis and sclerosis of the diaphyses of long
bones. The disease typically presents in early childhood with pain, muscular weakness and waddling gait, and in some cases other
features such as exophthalmos, facial paralysis, hearing difficulties and loss of vision.
Sequence similarities Belongs to the TGF-beta family.
Post-translational
modifications
Glycosylated.
The precursor is cleaved into mature TGF-beta-1 and LAP, which remains non-covalently linked to mature TGF-beta-1 rendering it
inactive.
Cellular localization Secreted > extracellular space > extracellular matrix.
Target information above from: UniProt accession P01137 The UniProt Consortium
The Universal Protein Resource (UniProt) in 2010
Nucleic Acids Res. 38:D142-D148 (2010).
Properties
Purity >95% by SDS-PAGE
Purification notes Greater than 98% by SDS-PAGE and HPLC analyses.
Biological activity Biological Activity : The ED50 was determined by TGF beta 1's ability to inhibit the mouse L4-dependent proliferation of mouse
HT-2 cells is < 0.05 ng/ml, corresponding to a specific activity of >2 x 107 units/mg.
Form
Lyophilised:
Centrifuge the vial prior to opening. Reconstitute in 10mM Citric Acid, pH3.0 to a concentration of 50 g/ml (e.g. 5 g/100l). This
solution can then be stored at room temperature or at 4oC for 1 month. t is recommended that further dilutions be made in PBS
containing 2 mg/ml albumin and stored at -20oC for future use.
Storage instructions Shipped at 4C. Upon delivery aliquot and store at -20C. Avoid freeze / thaw cycles.
Storage buffer Preservative: None
Endotoxin level is less than 0.1 ng per g (1EU/g).
Sequence notes ALDTNYCFSS TEKNCCVRQL YDFRKDLGW KWHEPKGYH ANFCLGPCPY WSLDTQYSK VLALYNQHNP GASAAPCCVP
QALEPLPVY YVGRKPKVEQ LSNMVRSCK CS
AppIications
SDS-PAGE SDS-PAGE :Use at an assay dependent dilution.
Functional Studies FuncS :Use at an assay dependent dilution.
http://www.abcam.com/TGF-beta-1-protein-ab50036.html 5 References |
UK office:
Abcam plc
330 Cambridge Science Park Cambridge CB4 0FL UK
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 771600
Email: orders@abcam.com
US office:
Abcam nc.
1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA
Tel: 617 225 2272
Fax: (617) 849-5829
Email: us.orders@abcam.com
Last updated
on October
28, 2011
Abpromise
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Quality is important to Abcam. f this antibody does not perform as described on this datasheet, notify us within 120 days of delivery using the online form, so that we can
help you or offer you a replacement or a refund.
UK office:
Abcam plc
330 Cambridge Science Park Cambridge CB4 0FL UK
Tel: +44 (0)1223 696000
Fax: +44 (0)1223 771600
Email: orders@abcam.com
US office:
Abcam nc.
1 Kendall Square, Suite B2304 Cambridge, MA 02139-1517 USA
Tel: 617 225 2272
Fax: (617) 849-5829
Email: us.orders@abcam.com
Last updated
on October
28, 2011