AC2-L User Service Manual Version 5.1 (10 May 2010)

Esco Controlled Environment Laboratory and Cleanroom Equipment Solutions
NSF / ANSI 49 Biological Safety Cabinets Animal Containment Workstations Fume Hoods Clean Benches
Class ll Biological Safety Cabinets
User and Service Manual
Thank you for purchasing this Esco Biological Safety Cabinet. Please read this manual thoroughly to familiarize yourself with the many unique features and exciting innovations we have built into your new equipment. Esco provides many other resources at our website, www.escoglobal.com, to complement this manual and help you enjoy many years of productive and safe use of your Esco products.
For Technical Service, contact North America Esco Technologies, Inc. 2940 Turnpike Drive, Units 15-16 Hatboro, PA 19040, USA Toll-Free USA and Canada 877-479-ESCO Tel 215-441-9661 Fax 215-441-9660 us.escoglobal.com usa@escoglobal.com Rest of World Esco Micro Pte. Ltd. 21 Changi South Street 1 Singapore 486 777 Tel +65 6542 0833 Fax +65 6542 6920 www.escoglobal.com mail@escoglobal.com
User And Service Manual

Class ll Biological Safety Cabinets
US $ 50.00 Europe 40.00 Additional manuals can be purchased through your Esco Distributor
Esco AC2-L User & Service Manual (Version 5.1) Released 10 May 2010
Copyright Information
Copyright 2010 Esco Micro Pte. Ltd. All rights reserved. The information contained in this manual and the accompanying product is copyrighted and all rights are reserved by Esco Esco reserves the right to make periodic minor design changes without obligation to notify any person or entity of such change. Sentinel and AirstreamMax are registered trademarks of Esco
Material in this manual is provided for informational purposes only. The contents and the product described in this manual (including any appendix, addendum, attachment or inclusion), are subject to change without notice. Esco makes no representations or warranties as to the accuracy of the information contained in this manual. In no event shall Esco be held liable for any damages, direct or consequential, arising out of or related to the use of this manual.
Class II Biological Safety Cabinet User and Service Manual
Table of Contents
Table of Contents
Warranty Terms and Conditions..................................................................................................... v
1. Products Covered.................................................................................................................................................. vii 2. Safety Warning..................................................................................................................................................... vii 3. Limitation of Liability........................................................................................................................................... vii 4. European Union Directive on WEEE and RoHS. ................................................................................................ viii 5. Symbols. ................................................................................................................................................................ viii
Declaration of Conformity............................................................................................................. ix About Esco Biological Safety Cabinets......................................................................................... xi
User Section
1. Basic Product Information........................................................................................................ 1
1.1 Unit Identification Labels. ............................................................................................................................1 1.2 Quick View....................................................................................................................................................2
1.2.1 Airstream Max Model AC2-L.......................................................................................................................... 2
1.3 Airflow Pattern Inside the BSC....................................................................................................................3

1.3.1 BSC Filtration System...................................................................................................................................... 3
2. Installation................................................................................................................................. 5
2.1 Pre-requisites.......................................................................................................................................... 5
2.1.1 Selecting the Installation Location. ................................................................................................................. 5 2.1.1.1 The following requirements should be taken into account: ................................................................ 5 2.1.2 Preparing For Installation. ................................................................................................................................ 7 2.1.2.1 Support Requirements........................................................................................................................ 7 2.1.2.2 Exhaust Requirements......................................................................................................................... 8 2.1.2.3 Electrical Requirements....................................................................................................................... 8 2.1.2.4 Service Line Requirements................................................................................................................... 8 2.1.3 Optional Retrofit Kits. ...................................................................................................................................... 9
2.2 Lifting and Placement on a Support Stand................................................................................................9 2.3 Connecting the BSC....................................................................................................................................10
2.3.1 Connecting the Electrical Supply................................................................................................................... 10 2.3.2 Connecting the Service Fixture(s). .................................................................................................................. 10 2.3.3 Connecting to an Exhaust System................................................................................................................. 10 2.3.4 Safety and Warning Labels............................................................................................................................ 12 2.3.5 Preliminary Cleaning..................................................................................................................................... 12
2.4 Performance Validation / Certification.....................................................................................................13

2.3.1 Disclaimer. ..................................................................................................................................................... 13
3. Operating Your BSC. ......................................................................................................................15

3.1 Sentinel Gold Control System Overview...............................................................................................15 3.2 Sash Window. ..............................................................................................................................................17
3.2.1 Sash Windows State...................................................................................................................................... 17
ii
Table of Contents
3.2.2 Operating Motorized Sash Window............................................................................................................... 17 3.2.3 Using Sash Window....................................................................................................................................... 18
3.3 Starting the BSC..........................................................................................................................................18

3.3.1 Turning on the BSC........................................................................................................................................ 19
3.4 Working in the BSC. ....................................................................................................................................19

3.4.1 Handling Spillage........................................................................................................................................... 20 3.4.2 Alarms and Warnings. .................................................................................................................................... 20
3.5 Working Ergonomics..................................................................................................................................21 3.6 Cleaning and Shutting Down the BSC......................................................................................................22
3.6.1 Turning off the BSC. ....................................................................................................................................... 22 3.6.2 Using UV Lamp (When Present)..................................................................................................................... 22 3.6.3 Disinfecting Agents........................................................................................................................................ 23
3.7 Menu Options.............................................................................................................................................25

3.7.1 Settings. ......................................................................................................................................................... 26 3.7.1.1 Set Clock (Time)................................................................................................................................. 26 3.7.1.2 Warm Up Timer................................................................................................................................. 27 3.7.1.3 Post Purge Timer................................................................................................................................ 28 3.7.1.4 UV Timer. ........................................................................................................................................... 29 3.7.1.5 Airflow Unit Selection........................................................................................................................ 30 3.7.1.6 Temperature Unit Selection................................................................................................................ 31 3.7.2 Calibration..................................................................................................................................................... 31 3.7.3 Admin. ........................................................................................................................................................... 32 3.7.3.1 New Admin PIN................................................................................................................................. 32 3.7.3.2 New Fan PIN...................................................................................................................................... 33 3.7.3.3 A/F Monitor....................................................................................................................................... 34 3.7.3.4 Reset Blower Hour Meter................................................................................................................... 35 3.7.3.5 Reset UV Hour Meter......................................................................................................................... 36 3.7.3.6 Reset Default..................................................................................................................................... 37 3.7.4 Setting the Mode........................................................................................................................................... 37 3.7.4.1 Normal Mode.................................................................................................................................... 37 3.7.4.2 Maintenance Mode. ........................................................................................................................... 37
4. Maintaining Your BSC............................................................................................................. 39

Log Record. ........................................................................................................................................................41
Service Section
1. Re-Certification and Maintenance by Service Personnel.................................................... 45
1.1 Certification Flowchart. ..............................................................................................................................46 1.2 Airflow Adjustment....................................................................................................................................46
Table of Contents
iii
1.3 Unit Re-certification...................................................................................................................................48 1.4 Replacement of Filters. ...............................................................................................................................60

1.4.1 Replacement of Filters for AC2-L BSCs........................................................................................................... 60
1.5 Replacement of Blower for AC2-L BSCs....................................................................................................62

1.5.1 Replacement of Primary Blower..................................................................................................................... 62
1.6 Replacement of Airflow Sensor. ................................................................................................................63

1.6.1 Replacement of Airflow Sensor for AC2-L BSCs . ........................................................................................... 63
1.7 Replacement of Flourescent Lamp and UV Lamp....................................................................................64 1.8 Decontamination Procedure. .....................................................................................................................64
1.8.1 Decontamination Agents............................................................................................................................... 64 1.8.1.1 Formalin/Paraformaldehyde Decontamination. ................................................................................. 64 1.8.1.2 Chlorine Dioxide Decontamination. .................................................................................................. 66 1.8.1.3 Hydrogen Peroxide Decontamination............................................................................................... 66 1.8.1.4 Comparison Table............................................................................................................................ 67 1.8.2 Formalin/Paraformaldehyde Decontamination Process................................................................................... 67 1.8.2.1 Common List of Equipment Used.................................................................................................... 67 1.8.2.2 Appropriate Protective Clothing....................................................................................................... 68 1.8.2.3 Common Apparatus........................................................................................................................ 68 1.8.2.4 Common Preparatory Steps............................................................................................................. 69 1.8.2.5 Sealing Methods.............................................................................................................................. 70 1.8.2.6 Decontamination Bag Method (without ducting)............................................................................. 70 1.8.2.7 Decontamination Bag Method (with ducting).................................................................................. 74 1.8.2.8 Decontamination Process................................................................................................................. 75 1.8.3 Completion Steps.......................................................................................................................................... 79 1.8.4 Validation of Decontamination Procedure (Optional). ..................................................................................... 79
2. Troubleshooting. ...................................................................................................................... 81

2.1 Diagnostic Read Out. ..................................................................................................................................81

2.1.1 Diagnostic Read Out in Normal Mode............................................................................................................ 81 2.1.2 Diagnostic Read Out in Maintenance Mode................................................................................................... 81
2.2 Electrical and Mechanical Troubleshooting. .............................................................................................82 2.3 Sash Position Detection............................................................................................................................107 2.4 Operation Mode Summary......................................................................................................................108 2.5 Software Troubleshooting.......................................................................................................................109
iv
Table of Contents
3. Product Specification............................................................................................................ 111

3.1 Airstream Max Model AC2 (L-Series) Engineering Details....................................................................111 3.2 AC2-L Series General Specifications........................................................................................................112 3.3 Environmental and Electrical Requirements..........................................................................................113
Appendix
Appendix A: Electrical Schematic Drawing Appendix B: Replacement Parts List Appendix C: Further Information and Reference Materials Appendix D: Log Record
Warranty Terms and Conditions

Esco warrants that this equipment will perform according to specifications for 3 years from the date of purchase. Escos limited warranty covers defects in materials and workmanship. Escos liability under this limited warranty shall be, at our option, to repair or replace any defective parts of the equipment, provided if proven to the satisfaction of Esco that these parts were defective at the time of being sold, and that all defective parts shall be returned, properly identified with a Return Authorization. This limited warranty covers parts only, and not transportation/ insurance charges. This limited warranty does not cover: Freight or installation (inside delivery handling) damage. If your product was damaged in transit, you must file a claim directly with the freight carrier. Products with missing or defaced serial numbers Products for which Esco has not received payment Problems that result from: External causes such as accident, abuse, misuse, problems with electrical power, improper operating environ mental conditions Servicing not authorized by Esco Usage that is not in accordance with product instructions Failure to follow the product instructions Failure to perform preventive maintenance Problems caused by using accessories, parts, or components not supplied by Esco Damage by fire, floods, or acts of God Customer modifications to the product Consumables such as filters (HEPA, ULPA, carbon, pre-filters) and fluorescent / UV bulbs Factory installed, customer specified equipment or accessories are warranted only to the extent guaranteed by the original manufacturer. The customer agrees that in relation to these products purchased through Esco, our limited warranty shall not apply and the original manufacturers warranty shall be the sole warranty in respect of these products. The customer shall utilise that warranty for the support of such products and in any event not look to Esco for such warranty support. Esco encourages all users to register their equipment online at www.escoglobal.com/warranty or complete the warranty registration form included with each product. ALL EXPRESS AND IMPLIED WARRANTIES FOR THE PRODUCT, INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES AND CONDITIONS OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE LIMITED IN TIME TO THE TERM OF THIS LIMITED WARRANTY. NO WARRANTIES, WHETHER EXPRESS OR IMPLIED, WILL APPLY AFTER THE LIMITED WARRANTY PERIOD HAS EXPIRED. ESCO DOES NOT ACCEPT LIABILITY BEYOND THE REMEDIES PROVIDED FOR IN THIS LIMITED WARRANTY OR FOR SPECIAL, INDIRECT, CONSEQUENTIAL OR INCIDENTAL DAMAGES, INCLUDING, WITHOUT LIMITATION, ANY LIABILITY FOR THIRD-PARTY CLAIMS AGAINST YOU FOR DAMAGES, FOR PRODUCTS NOT BEING AVAILABLE FOR USE, OR FOR LOST WORK. ESCOS LIABILITY WILL BE NO MORE THAN THE AMOUNT YOU PAID FOR THE PRODUCT THAT IS THE SUBJECT OF A CLAIM. THIS IS THE MAXIMUM AMOUNT FOR WHICH ESCO IS RESPONSIBLE.
These Terms and Conditions shall be governed by and construed in accordance with the laws of Singapore and shall be subject to the exclusive jurisdiction of the courts of Singapore.
vi
Technical Support, Warranty Service Contacts Toll-Free USA and Canada 877-479-ESCO Singapore: +65 6542 0833 Global Email Helpdesk: support@escoglobal.com Visit http://www.escoglobal.com/ to talk to a Live Support Representative Distributors are encouraged to visit the Distributor Intranet for self-help materials. Policy updated on 30th Jan 2007 (This limited warranty policy does not apply to products purchased before 30th Jan 2007).
Introduction
vii
1. Products Covered This manual is applicable and specific to the following Esco products. Product Name 1.2 meters (4)
AC2-4L1
Electrical 220-240V, AC, 50Hz, 1
1.8 meters (6)

AC2-6L1
Note: The AC2-_L1 (220-240V, AC, 50Hz, 1 ) are tested and certified with the European BS EN 12469:2000.
2. Safety Warning Anyone working with, on or around this equipment should read this manual. Failure to read, understand and follow the instructions given in this documentation may result in damage to the unit, injury to operating personnel, and / or poor equipment performance. Any internal adjustment, modification or maintenance to this equipment must be undertaken by qualified service personnel. The use of any hazardous material in the cabinet must be monitored by an industrial hygienist, safety officer or some other suitably qualified individual. Explosive or inflammable substances should never be used in the cabinet unless a qualified safety professional has evaluated the risk involved. If chemical, radiological or other non-microbiological hazards are being used in the cabinet, additional protective measures should be taken. Besides that, the operation should be monitored by a suitably trained individual. The biological hazard symbol (shown alongside) on the front panel of the cabinet indicates the presence of biological substances that pose a threat to human health. This cabinet is suitable for agents classified under biological safety levels 1, 2 and 3. Please refer to Introduction section in this user manual for more information on biological safety levels. For use of biological safety level 4 agents in the cabinet, the agents should be placed in a negative pressure area. Please note that while doing so, the operator should be wearing a full body positive pressure isolation suit. This cabinet should not be used with cytotoxic substances unless it has been determined that the filter can be safely changed. NB: Cytotoxic substances cannot be inactivated by conventional formaldehyde decontamination
Before you proceed, you should thoroughly understand the installation procedures and take note of the environmental/electrical requirements of the cabinet In this manual, important safety related points will be marked with this symbol. If the equipment is used in a manner not specified by this manual, the protection provided by this equipment may be impaired.
3. Limitation of Liability The disposal and / or emission of substances used in connection with this cabinet may be governed by various local regulations. Familiarization and compliance with any such regulation are the sole responsibility of the users of the cabinet. Escos liability is limited with respect to user compliance with such regulations.
viii
Introduction
4. European Union Directive on Weee and RoHS The European Union has issued two directives: Directive 2002/96/EC on Waste Electrical and Electronic Equipment (WEEE) This product is required to comply with the European Unions Waste Electrical & Electronic Equipment (WEEE) Directive 2002/96/EC. It is marked with the following symbol: Esco sells products through distributors throughout Europe. Contact your local Esco distributor for recycling/disposal. Please note that for contaminated devices (example biological safety cabinets), it is the responsibility of the user to ensure the product is decontaminated prior to disposal and to provide Certificate of Decontamination to the Esco distributor/disposal agency. Directive 2002/95/EC on Restriction on the use of Hazardous Substances (RoHS). With respect to the directive on RoHS, please note that this cabinet falls under category 8(medical devices) and category 9 (monitoring and control instruments) and is therefore exempted from requirement to comply with the provisions of this directive.
5. Symbols Information in this manual may be prefaced with the following symbols. They are provided to help you identify important operational, safety, maintenance or conformance issues.
Electrical Hazard: Danger of electric shock
Turn Off And Disconnect From Main Supply Before Proceeding: Do not perform this operation while the unit is operational
The Biohazard Symbol on the front panel of the cabinet indicates the presence of biological substances that pose a threat to human health
Important Information: Read and understand before proceeding. Important safety related points will be marked with this symbol
Approved Service Engineer Only: Operation to be performed only by approved engineer
Declaration of Conformity
ix
Declaration of Conformity *
In accordance with EN 45014:1998
We, based at
Esco Micro Pte Ltd 21 Changi South Street 1 Singapore 486777 Tel: +65 65420833 Fax: +65 65426920
declare on our sole responsibility that the product: Equipment Model : Biological Safety Cabinet : Airstream Max Class II (AC2-4L & AC2-6L)
in accordance with the following directives: 2006/95/EEC : The Low Voltage Directive and its amending directives 89/336/EEC : The Electromagnetic Compatibility Directive and its amending directives
has been designed to comply with the requirements of the following Harmonized Standard: More information may be obtained from Escos authorized distributors located within the EU. A list of these parties and their necessary contact information is available on request from Esco. Low Voltage EMC Design/ Performance Criteria : EN 61010-1 (2001) : EN 61326-1 (2002) Class B : EN 12469 (2000) Class II Biological Safety Cabinet
Lim Lay Yew Chief Executive Officer
* Applicable only to 220-240V, AC, 50 Hz cabinets
About Esco Biological Safety Cabinets
xi
Introduction
The biological safety cabinet plays a significant role in many pharmaceutical, clinical, microbiological and industrial laboratories. Its basic function is to protect the operator and the environment from biological hazards that would otherwise pose a threat to human life during microbiological work. At Esco, we encourage customers to learn more about the functions and operating principles of the biological safety cabinets available in the market today. We believe this will help you chose the cabinet that is most suitable for your specific needs.
Major International Standards International standards play an important role in harmonization and ensuring that cabinets meet established industry guidelines for safety and performance. Consequently, you should understand the scope and application of these standards. The following tables detail some common international standards: The different nomenclature used by various standards and the inflow requirement
Standard origin NSF / ANSI 49 NSF / ANSI 49 JIS K 3800:2000 EN 12469:2000 AS 2252 The standard nomenclature USA Class II Type A1 USA Class II Type A2 Japan Class II Type A/B3 European Union Class II Australia Class II Minimum inflow requirement (m/s) 0.38 0.50 0.40 (Type A) / 0.50 (Type B3) 0.40 To pass air barrier test
Bio Safety Level (BSL)

Lethality 1 2 3 4 Safe Some Serious Extreme Medium Liquid Liquid Airborne Airborne Cure Yes Some Some None Example Bacillus Subtilis HIV TBC Ebola
Name / Number
NSF / ANSI 49
Governing Body
The National Sanitation Foundation (NSF) International (http://www.nsf.org/)
Referenced
US / Global Applies to / Covers: Class II biological safety cabinets
Notes: Standard 49 has been widely adopted not only in the United States but also around the world.
Name / Number
EN 12469:2000
Governing Body
International Organization for Standardization (http://www.iso.org)
Referenced
EU / Global Applies to / Covers: Class I biological safety cabinets Class II biological safety cabinets Class III biological safety cabinets
Notes: The European Norm 12469 is the latest international standard developed as a direct consequence of the European Unions harmonization efforts. It replaces previous standards such as the German standard DIN 12950, the British standard BS 5726 and the French standard NF X44-201. The EN 12469:2000 has been adopted officially as a national standard in all member nations of the EU.
Name / Number
AS 2252
Governing Body
Standards Australia (http://www.standards.com.au/)
Referenced
Australia / New Zealand
Notes: The AS 2252 is the Australian standard for biological safety cabinetry. It has also largely been Applies to / Covers: adopted as a national standard in nearby New Zealand. It applies to both Class I and Class II cabi- Class I biological safety cabinets netry with a focus on cabinet construction, design and performance. Australian standard cabinets Class II biological safety cabinets incorporate twin blowers and are largely different from European and American type models.
xii
About Esco Biological Safety Cabinets
Name / Number
JIS K 3800:2000
Governing Body
Japanese Industrial Standards Committee (http://www.jisc.go.jp/eng/)
Referenced
Japan
Notes: Applies to / Covers: The JIS K 3800 was derived from the NSF49, and used only in Japan. Just like NSF 49, JIS K 3800 Class II biological safety cabinets only applies to Class II biological safety cabinets.
Biological Safety Cabinet Summary Table of Features Class (Type) Biosafety Level
123 I
Protection Offered Operator
Product
Min. Inflow Velocity

0.38 m/s / 75 fpm
Recirc. Exhaust Air Air

0% 100%
Contaminated Plenum Surrounded By

Outside air (Lab room)
Exhaust Alternatives
Inside room / Hard Duct
Basic design and feature set Unfiltered room air flows into the cabinet, passes through the workzone and then through a filtration system, trapping airborne contaminants. Clean, decontaminated air is exhausted from the cabinet. The scope and application of Class I cabinets is limited and it is largely considered obsolete. 123 0.38 m/s / 75 fpm 70% 30% Outside air (Lab room) Inside room / Thimble Duct
II A1
Room air enters the cabinet through a front inlet grille and is filtered before entering the workzone, contaminated air is passes through a filtration system trapping airborne contaminants. Clean, decontaminated air is recirculated through the workzone or exhausted from the cabinet. Type A1 cabinets have a positively-pressurized plenum bordering the ambient environment. Type A1 is now largely considered obsolete 123 0.50 m/s / 100 fpm 70% 30% Negative pressure Inside room / Thimble Duct
II A2
Negative pressure surrounds the positively pressurized contaminated plenum providing added protection in the case of a leak in the positively pressurized plenum. Most European, Japanese, and Australian Class II cabinets have airflow recirculation and exhaust ratios similar to Class II Type A2 cabinet along with the negative pressure isolated plenum operating in the same principle as the NSF Class II Type A2, but with lower inflow velocity requirement. 123 0.50 m/s / 100 fpm 30% 70% Negative pressure Hard duct only
II B1
Type B1 cabinets also have a dedicated exhaust feature for work done inside the cabinet towards the back. Toxic chemicals should only be used if they do not interfere with work when re-circulated in the downflow. Type B1 cabinets have largely been replaced by Type B2, they are now generally considered obsolete. 123 0.50 m/s / 100 fpm 0% 100% Negative pressure Hard duct only
II B2
Type B2 cabinets exhaust all inflow and downflow air after HEPA filtration to the external environment. Type B2 cabinets are suitable for work with toxic chemicals used as an adjunct to microbiological processes. Type B2 cabinets may be considered to be the safest of all Class II biological safety cabinets in the sense that the total exhaust feature acts as a fail-safe against downflow and/or exhaust HEPA filtration malfunction. However, the cabinet requires a stable building exhaust system that is precisely tuned to the cabinet airflow requirements. 1234 P < -125 Pa P < -0.5 WG 0% 100% Negative pressure Inside room / Hard Duct
III
Class III cabinets are usually of welded metal construction and are designed to be gastight. Work is performed through glove ports in the front of the cabinet. During routine operation, negative pressure relative to the ambient environment is maintained within the cabinet. Class III cabinets provides product protection and prevent cross-contamination of samples. Exhaust air is usually HEPA filtered and incinerated. Alternatively, double HEPA filtration with two filters in series may be utilized. Class III cabinets usually exhaust air back to the laboratory, air may also be exhausted to the external environment. When a dedicated ductwork system is employed, they are suitable for working with toxic chemicals as an adjunct to microbiological processes. All Class III biological safety cabinets are suitable for work with microbiological agents assigned to biological safety levels 1, 2, 3 and 4. They are frequently specified for work involving the most lethal biological hazards.
Further Information For further information on the topics discussed above, we have many documents available our Technical Support library at www.escoglobal.com. Here you will find the most up-to-date information in far more detail than is possible to include in this manual.
USER SECTION
User Section Chapter 1 Basic Product Information
chapter 1 Basic Product Information

1.1 Unit Identification Labels
3
1. The first label will contain:
Model : The model of the unit. Serial : The serial number of the unit. Power : The maximum power consumption and the electrical requirement of the unit. Manufactured in.. : The month and year when the unit was manufactured.
2. The second label will contain:
The model of the unit Inflow : The inflow setpoint for the particular unit. Inflow velocity can be measured by using Direct Inflow Measurement (DIM) or by using the secondary inflow measurement method. Information on measuring using the Grid can be found in the third line. Downflow: The downflow setpoint for the particular unit and the total check points required. Downflow velocity can only be measured by using the Grid method as this method can also show the uniformity of the airflow. Information on measuring using the Grid can be found in the fifth line. Note: For further information about inflow and downflow measurement, refer to chapter 1 of the Service section.
3. The third label is usually located next to the Biological safety cabinet (BSC) power inlet. This label contains the electrical requirement needed to operate the unit.
1.2 Quick View 1.2.1 Airstream Max Model AC2-L

1
14 2
3 5 13 12 5 6 11 10
1. Optional exhaust collar for thimble-ducting. 2. Exhaust ULPA filter 3. Downflow ULPA filter 4. Downflow sensor 5. Fluorescent lamps 6. Tempered side glass 7. Stainless steel arm rest 8. Optional support stand (with leveling feet shown, other types and options are available). 9. Multi-piece work surface simplifies removal and cleaning. 10. Standard electrical outlet Retrofit Kit provision 11. Frameless, shatterproof sash is easier to clean, offers larger, unobstructed viewing area. 12. Esco Sentinel Gold microprocessor supervises all biosafety cabinet (BSC) functions. The control panel is located on the center of the cabinet, and angled down for easy access by the operator. 13. Blower 14. Exhaust inflow sensor above the exhaust filter (hidden)
Class II BSC provide product, operator and environmental protection. They are suitable for general microbiological work with agents classified under biological safety levels 1, 2 or 3. (For more details please refer to www.escoglobal.com).
1.3. Airflow Pattern Inside the BSC 1.3.1 BSC Filtration System
Dynamic air barrier, inflow and forward-directed downflow air converge
ULPA - filtered air Unfiltered / potentially contaminated air Room air / inflow air
Ambient air is pulled through the perforations located towards the work zone front to prevent contamination of the work surface and work product. The inflow does not mix with the clean air within the BSC work zone. Inflow air travels through a return path toward the common air plenum (blower plenum) at the top of the BSC. The uniform, non-turbulent air stream protects against cross contamination within and throughout the work area. Near the work surface, the downflow air stream splits with a portion moving toward the front air grille, and the remainder moving to the rear air grille. A small portion of the ULPA filtered downflow enters the intake perforations at the side capture zones at a higher velocity (small blue arrows). A combination of inflow and downflow air streams forms an air barrier that prevents contaminated room air from entering the work zone, and prevents work surface emissions from escaping the work zone. Air returns to the common air plenum where the 32% exhaust and 68% recirculation process is continued.
User Section Chapter 2 Installation
chapter 2 Installation
2.1 Pre-Requisites 2.1.1 Selecting the Installation Location Placing the BSC in a proper location is important. Bad location may affect the performance of the BSC. As seen in the chart, your BSCs internal airflow velocity is relatively low, when compared to the airflow disturbances potentially caused by the opening of a door, a person walking by or a direct exposure to an airconditioning outlet. These external airflow disturbances can affect the proper laminar flow of the BSC and impaired the protection offered by the BSC. When installing the BSC, it should be located as far away as possible from the above-mentioned sources of airflow disturbance and in an orientation which optimally shields the BSCs internal airflow from all external airflow disturbances. Please note that the BSC should not be placed close to another BSC.
Relative air velocities of airflow disturbances
2.1.1.1 The following requirements should be taken into account: a. Poor siting of a cabinet can adversely affect performance. A specialist should be consulted on correct positioning of the BSC prior to installation. b. BSCs should never be sited in line with a doorway, an openable window, or adjacent to a thoroughfare. Care should be taken to ensure that possible disturbances to airflow such as room air diffusers, fans, extractors, vents, etc. are taken into account and any risk of disturbance noted and mitigated before installation. c. Room air supply diffusers should not be within 1.5 meters (5) of the front aperture. If there are large numbers of BSCs in a laboratory this recommendation may be difficult to comply with, but where diffusers have to be placed in close proximity to a safety BSC, their discharge velocities and therefore air handling rates will need to be low. d. The position of a safety BSC should satisfy the spatial requirements (e.g. vision, lighting and convenience of access) of the operator and people working nearby. If the BSC is installed on a bench top, the leading edge should slightly overhang or be flush with the edge of the bench top. e. There should not be an open space between the leading edge of the BSC and the front of the bench as this may create turbulence in front of the aperture. It also provides an obstacle which could adversely affect airflow across the BSC face.
B S C
1 m (3 ft)
Any pedestrian traffic routes, thoroughfares or walkways should be at least 1.0 meter (3) from the front of the BSC, so as to preserve a zone undisturbed by anyone other than the operator.
You should not position the BSC with either side closer than 30 cm (1) to adjacent walls or other similar obstructions. Allow at least 30 cm (1) clearance on both sides of the BSC. There should be adequate space left for cleaning the sides of the BSC and for carrying out decontamination procedures. There should be unobstructed access to the main power supply point(s).
B S C
30 cm (1 ft)
B S C
You should not position the BSC where the distance between the aperture and any doorway is less than 1.5 meters (5) or the distance between the side panel and any doorway is less than 1.0 meter (3). Door openings cause substantial air turbulence. If the door is fitted with air transfer grills, operator protection factor testing may be carried out to determine suitable reduced clearance.
1.5 m (5 ft)
Safety cabinets should not be installed in positions where there is a likelihood of interference from other laboratory equipment. The distance from the aperture to the aperture of an opposing BSC, fume cupboard, etc. should be in excess of 3 meters (10) for safe operation.
B S C
3 m (10 ft)
B S C
COLUMN
30 cm (1 ft)
B S C
As with walls, any large obstruction such as a pillar or column projecting beyond the plane of the front aperture should not be within 30 cm (1) of the sides of the BSC.
You should not position the BSC in a location where there is an opposing wall (or other obstruction likely to affect airflow) within 2 meters (7) of the front aperture.
B S C
2 m (7 ft)
B S C
1.5 m (5 ft)
BENCH
The distance between the aperture of the BSC and the front of a bench opposite should not be less than 1.5 meters. (5) Containment performance may not be affected if this distance is reduced, to allow the operator to use the bench whilst working in the BSC for instance. You should measure any such effects using relevant operator protection factor tests to determine safety limits.
Avoid positioning a bench at right angles to the BSC. Whilst this may reduce traffic in front of the BSC, any other person working at the bench is likely to disturb airflow close to the BSC.
B S C BENCH
B S C
1 m (3 ft)
A projecting bench will help minimize traffic in front of the BSC and anyone working at the bench is unlikely to have a significant effect on the airflow as long as the front of the bench is situated at least 1 meter (3) from the side of the BSC.
BENCH
2.1.2. Preparing for Installation 2.1.2.1 Support Requirements Esco provides a number of support stand options, these are summarized below. Fixed height Adjustable height Telescoping height Infinitely adjustable cradle stand
Esco support stand with levelling feet is recommended for biological safety BSCs. It is recommended that the installation of the support stand be carried out by qualified personnel (contact your Esco Distributor for assistance). After installation of the BSC on the support stand, using a level placed in the centre of the work tray, adjust the legs to achieve a level work surface. First level from left to right and then from front to back. The NSF approved leg levelers provide a maximum of 50 mm (2) adjustment.
Two persons would be needed for assembling the support stand as it is quite heavy. Should the BSC be relocated after the initial installation, take all the necessary precautions as the BSC is very heavy. If the BSC is not installed on Escos optional support stand, Esco can not guarantee BSCs resistance against tipping and hence the user would be solely responsible for ensuring that the BSC is securely fastened to third part stand or table. The use of non-leveling feet Esco support stand will nullity the third party certification (NSF or TV) that the BSC may have, because only Esco leveling feet support stand was used during certification. While installing the BSC onto an existing work surface, ensure that the structure can safely support the combined weight of the BSC and any related equipment. Some modifications to the work surface may be necessary. The work surface should be smooth, nonporous and resistant to those disinfectants and chemicals, to which the BSC is regularly exposed.
2.1.2.2 Exhaust Requirements The exhaust filter area is especially susceptible to disruptive air currents or air drafts. A clearance of at least 30 cm (1) is recommended between the highest point of the BSC and the ceiling. If the distance is less than 30 cm (1), the airflow alarm system may need re-calibration. In fact, for proper exhaust filter leak scanning purposes, a minimum clearance of 50 cm (18) is recommended. If you intend to connect your BSC to an external exhaust system, Esco offers an optional Exhaust Collar for ThimbleDucting. Installation requirements and instructions are provided with the Exhaust Collar. 2.1.2.3 Electrical Requirements The BSC should be connected to its own dedicated power outlet(s). The power rating for each model is shown in section 3.4 Environmental and Electrical Requirements in the Product Specification Section. Ensure that the outlet is rated accordingly. The power cable is located on the right hand side of the BSC and the cord is 2.5 m long. When preparing the installation site try to ensure the outlet is located to the right of the BSC for ease of access. Accuflow G2 speed controller required that the unit has a proper grounding. To check if your electrical system has a proper grounding do this simple measurement test to the socket of the main AC power line: L-N should be close to 230V for 230V line or 240V for 240 line, 115V for 115V line. L-G should be close to 230V for 230V line or 240V for 240 line, 115V for 115V line. N-G should be close to zero or well below 10V if the building AC supply quality is not good. Accuflow G2 speed controller required that the units electrical connection has the correct polarity. 2.1.2.4 Service Line Requirements All service lines should be installed by a suitably qualified and certified engineer, in accordance with all applicable local, state and government regulations. Service line attachments should be equipped with an emergency shut off valve that can be accessed quickly and with ease, should the need arise. You should check with your local service installer as to whether there is a need to install pressure regulators to reduce the line pressure. Your BSC can accommodate service fixtures on the left or right hand side of the BSC. Make allowance for the positioning of service lines when planning the installation site to ensure ease of access to emergency shut off valves.
2.1.3 Optional Retrofit Kits Full instructions for optional retrofit kits are included with the kit. Please refer to the manual that accompanies the kit for installation instructions. Following is a list of retrofit kits available for this unit, you may also want to visit www. escoglobal.com for more information. Accessories and Options Esco offers a variety of options and accessories to meet local applications. Contact Esco or your local sales representative for ordering information. Accessory / Option Electrical Outlets and Utility Fittings Description Electrical outlet, ground fault, North America Petcock (air, gas, vacuum) - North America (American) style - Euro/Worldwide style DIN12898, DIN12919, DIN 3537 Fixed height, available 737 mm (29) or 838 mm (33), 38.1 mm (1.5) - With leveling feet - With casters Adjustable height, hydraulic range from 737 mm (29) to 838 mm (33) - Manual or electrical lift - With casters. Only this type of support stand, with leveling feet, was tested by third party (NSF, TUV) certification that the BSC may have. Telescoping height, nominal range from 737 mm (29) to 838 mm (33) - Adjustable in 25.4 mm (1) increments Infinitely adjustable cradle stand, with casters - Elevates to seating or standing work surface height - When lowered, permits movement through standard doorway. Note: Increases exterior dimensions. PVC armrest - Chemically treated, improves operator comfort, easy-to-clean, 712 mm (28) standard size Ergonomic lab chair - Laboratory grade construction, meets Class 100 cleanliness; alcohol resistant PVC materials - Adjustable 395-490 mm (15.6-19.3) Germicidal UV lamp - Controlled by automatic UV lamp timer through Sentinel microprocessor control panel - Emission of 253.7 nanometers for most efficient decontamination - Lamp is positioned away from operators line-of-sight for safety and proper exposure to interior surfaces. Note: UV lamp intensity reduces over time and its effectiveness is subject to factors such as relative humidity in the BSC, ambient air temperature and microbial species in he work zone. Ergonomic foot rest - Angled, helps maintain proper posture - Adjustable height - Anti-skid coating, chemical resistant finish IV Bar, with hooks - Stainless steel construction - Available for all standard BSCs
Support Stands
BSC Accessories
2.2 Lifting and Placement on a Support Stand Before moving and installing the unit, check the clearance for width and height of the passage to the final location of the unit to be installed (elevators, doors, etc) Assemble the support stand accordingly. Instructions on the Esco support stand can be found inside the box, with the support stand. The unit is TOP heavy. Extra care must be taken so it will not topple while lifting or carrying it. Use a stacker, when available, to slowly lift the unit up and place it on the pre-assembled support stand. If no stacker is available, a minimum of around 6 men is needed to safely lift up the unit. After placing it on the support stand, screw it up at the four corners.
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2.3 Connecting the BSC 2.3.1 Connecting the Electrical Supply Connect the supplied electrical cord to the input on the top of the BSC. Make sure the cable connector is seated firmly in the socket. 2.3.2 Connecting the Service Fixture(s) If you have purchased service fixtures for your BSC these will either have been factory installed or provided in a package located inside the work tray when you unpacked the BSC. If the fixtures have been provided for site installation there will be full instructions provided with them. Please refer to the instructions provided to install your retrofit kits. Connecting the BSC to service lines must be performed by a suitably qualified and certified personnel, in accordance with all applicable local, state and government regulations. Where applicable, each connection should be tested and certified by the installation personnel. Utility service lines recommendations: 1. Size of 3/8 outer diameter. 2. Soft metal material like brass, copper or stainless steel with blunted end. 3. Equip certified shut-off valve within easy reach. 4. Equip certified pressure regulator to high pressure line. 5. Ensure that the line is properly seated, secured and tighten. 6. Ensure the use of proper, good ferrule and materials. 7. Ensure that the line is not in contact with any electrical wires. 8. Avoid the use of combustible gases or solvents. Do careful evaluation, by safety personnel, in accordance to the national, state and local codes, if you are considering using the flammable substances. 9. Slowly open the shut-off valve and test for any leakages. Any seepage from a BSC may be biologically hazardous, so extra care must be taken installing the plumbing to the service fixtures. 2.3.3 Connecting to an Exhaust System If you intend connecting the BSC to an external exhaust system you will need an optional thimble (non air-tight) exhaust collar. 2.3.3.1 Exhaust Collar Installation Thimble exhaust collar/exhaust transition is used when a Class II (Type A2) biosafety cabinet is intended to be used for handling trace amount of chemical vapor that is potentially harmful to the user, if the vapor is exhausted into the lab. By using an exhaust system, chemical vapor is removed from the area above the exhaust ULPA filter to outside environment.
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1. Install the exhaust collar (Figures 1, 2, and 3):
Fig. 1 Exhaust collar, with slots only in front and back
Fig. 2 The collar must have 45 Air guide
Fig. 3 The airflow sensor cable should go through the cable slot on the exhaust collar
2. Connect the exhaust collar with the exhaust duct, then turn on the BSC fan and the exhaust fan. 3. Adjust the exhaust fan suction to be strong enough so that the smoke from the smoke tube can rapidly goes into the slots throughout their length, especially on the slot portion near the airflow sensor box (Figure 4)
Fig. 4 Smoke should be drawn rapidly into the slots, especially on the area near the airflow sensor box
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4. Measure the airflow coming into the exhaust collar inlets using thermo-anemometer, placed with vertical orientation pointing down (Figure 5), with the center of the sensor placed using the following grid:
Distance from outer points to walls Distance between one point to another 10 cm (4 in) 10 cm (4 in)
Fig. 5 Thermo-anemometer is placed vertically, with the center of the white tip at the center of the slot height. Mark the grid using pen and masking tape directly on the exhaust collar
5. Re-calibrate the airflow sensor. Because the airflow velocity through the sensor box is different after the staggered damper and exhaust collar were installed, the airflow sensor requires re-calibration. The approximate exhaust collar slot air velocity, total exhaust volume, and negative pressure needed:
No 1 2 BSC AC2-4L_ AC2-6L_ Velocity at Front Slot 1.53 m/s (301 fpm) 1.55 m/s (305 fpm) Velocity at Back Slot 1.54 m/s (303 fpm) 1.49 m/s (293 fpm) Exhaust Volume 480 cmh (283 cfm) 735 cmh (433 cfm) Pressure at 3 above duct connection -5 Pa (-0.02 WG) -21 Pa (-0.08 WG)
Note: that the values above are approximation, but the actual velocity and pressure shall be set as minimum required exhaust to ensure that the smoke ejected on the collar slots is rapidly drawn in. Having greater velocity than needed will increase containment, but if the velocity is too high, the downflow velocity may be weakened. 2.3.4 Safety and Warning Labels The two biohazard decals included with this manual should be fixed to the door leading to your biohazard Laboratory. 2.3.5 Preliminary Cleaning Wipe the interior and exterior of the BSC with water or a mild household detergent.
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2.4 Performance Validation / Certification After having installed the BSC but before starting to use it, BSC performance must be validated and certified to factory standards. It is recommended that this validation and certification be performed only by a qualified technician who is familiar with the methods and procedures for certifying biological safety BSCs. 2.4.1 Disclaimer The performance and safety of all Esco BSCs are rigorously evaluated at our factory. Regular field certification is important to ensure factory standards are maintained. References for Qualified Certifiers North America NSF (http://www.nsf.org/Certified/Biosafety-Certifier/) Esco (www.us.escoglobal.com) IAFCA member certifying company (www.iafca.com/listview) UK, China, India, Middle East/North Africa, Malaysia, Singapore Esco offers field certification services directly. Contact the local Esco office. Other Countries Contact Esco or local distributor
User Section Chapter 3 Operating Your BSC
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CHAPTER 3 Operating Your BSC

Proper operating practices are essential to achieve effective performance and protection. A properly functioning, certified BSC alone is not sufficient. This chapter provides information on operating procedures and recommended work practices to help users achieve the best performance and protection. 3.1 Sentinel Gold Control System Overview Esco Sentinel microprocessor-based control system controls and monitors operation of all BSC function. Fan, lamp, electricity socket, and UV can be controlled via the corresponding button. BSC airflow and sash position are monitored in real time. The user friendly menu allows adjustments of BSC operation parameters and contains helpful features for service personnel. Good work practices are just as important as a good unit itself in order to achieve effective containment of contaminants. This chapter is aimed at providing the users with information on basic operations of this unit, besides outlining various recommended work practices, so that they are able to achieve optimal containment from their Esco units.
Warning LED Up Arrow Menu Button Button Fan Button Light Button
Down Arrow Set Button Button
Socket Button
UV Button
1. Fan Button Turns on and off the fan. 2. Light Button Turns on and turn off the lamp. Lamp turned on automatically when sash is at the operating position, READY state. Lamp turned off automatically when sash is at SASH ALARM state. 3. Socket Button To turn on and turn off the electrical socket (when present). SOCKET can only be manually activated when fan is on, airflow is safe, and sash is at normal operating height. SOCKET switches off automatically when fan is off, or airflow is unsafe, or sash is not at normal operating height, or accessing MENU options. The maximum rating of all the outlets in the BSC is 5A. In case of overloading, the fuse F4 on relay board will blow. Contact Esco for replacement fuse.
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4. UV Button Turns on and turn off the UV lamp (when present). UV lamp can only be activated when the sash window is fully closed. Since the sash is capable of filtering UV rays, users are protected from the harmful UV rays. UV Timer is provided to control the duration of the decontamination process. Please refer to Section 3.7.1.4 for further information. UV Hour Meter is provided to monitor the working hours of UV lamp. Please refer to Section 3.7.3.5 for further information Avoid UV exposure to eyes and skin. Ensure the sash is fully closed before UV lamp is switched ON. Hazardous UV-C can be present internally. UV interlock should not be defeated at all times. 5. Up () and Down () Arrow Button To control (move up and down) the window. To move upwards and downwards the menu options. To increase and decrease corresponding value inside one of the menu options. 6. Set Button Choose the menu or sub-menu currently displayed on the LCD screen. Proceed to the next step or sequence inside one of the menu options. 7. Menu Button Alarm is disabled when you enter the menu options. To enter and exit from the menu options. To go back to the previous level of the menu options. To access maintenance mode from ERR.MSWITCH and AIRFAIL! error condition. Some of the menu options allow the user to customize the BSC for better working experience. Please kindly refer to Section 3.7 for detailed explanations of each menu options. Before operating the BSC, please ensure that you have set the Admin. PIN (0009 by default) and Fan PIN (0001 by default). The Admin. PIN has higher priority and can be used to control the fan (override the Fan PIN) too. Please contact Esco should you forget your Admin. PIN.
8. Warning LED Red warning LED illuminates when there is any unsafe condition: Airflow alarm, or sash position condition.
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3.2 Sash Window 3.2.1 Sash Windows State
Sash in fully
open positio
ight Sash in safe he position
sed Sash in fully clo position
Sash is fully open

Blower can be active Flourescent lights can be used UV light can not be used Unsafe working environment
Sash is at safe operating height

Blower can be active Flourescent lights can be used UV light can not be used Safe working environment
Sash is fully closed

Blower can not be active Flourescent lights can not be used UV light can be used Unsafe working environment
3.2.2 Operating Motorized Sash Window The motorized sash uses a push and hold mechanism, so if you remove your finger from the button the sash will stop immediately this is a safety feature to control the closure and prevent anything getting trapped in the aperture as the sash descends. Lower Sash From Fully Open Position When the sash is fully open, pressing the down arrow button () and holding it will cause the sash to move to the Safe Height setting and stop. If the fluorescent lights are on as the sash descends, they will stay on as long as the sash stops in the Safe Position. If you release the button before the sash has reached Safe Position the lights will switch off automatically. Lower Sash From Safe Height Position When the sash is in the safe height position, pressing the down arrow button () and holding it will cause the sash to move down to the fully closed position and stop. If the fluorescent lights are on as the sash descends, they will switch off automatically as soon as the sash reaches fully closed. If you release the button before the sash has reached the fully closed position the lights will switch off automatically. Raise Sash From Fully Closed Position When the sash is fully closed, pressing the up arrow button () and holding it will prompt the user to input the password to turn on the fan. If the password is correct, the UV lamp will be turned off, if it was on fan will turn on and the sash will move up to the Safe Height setting and stop. Raise Sash From Safe Height Position When the sash is safe operation position, pressing the up arrow button () and holding it will cause the sash to move up to the fully open position and stop. If the fluorescent lights are on as the sash rises, they will stay on as long as the sash is allowed to fully open. Stopping the sash midway will cause the lights to switch off automatically.
3.2.3 Using Sash Window 1. The sash window should be fully closed when the cabinet is not in use. This helps keep the work zone interior clean. The sash window also provides a protective barrier in case the UV lamp is used. 2. The sash window should always be in the normal operating height at all times when the cabinet is in use. Even if the cabinet is left unattended, but the blower is on, the sash window should never be moved from the normal operating height, unless during loading or unloading of materials/apparatus into the cabinet 3. The alarm will be activated whenever the sash window is moved from the normal operating height. 4. Whenever the sash window is moved to the correct height from a higher or lower position, the light will automatically be turned on as a signal to the user. 5. The sash window may be opened to its maximum position for the purpose of loading/unloading of materials/ apparatus into the cabinet. When the sash window is fully opened, the alarm sound may be muted by pressing MUTE Button but, will be automatically sounded again after 5 minutes to remind the user that it is not safe to work in the cabinet and the light will be turned on to facilitate cleaning. 3.3 Starting the BSC 1. Wash hands thoroughly using germicidal soap. Wear gloves for hand protection. Gloves should be pulled over the knitted wrists of the gown instead of wearing them inside. Double gloving may be necessary for higher risk work. 2. Put on a clean laboratory coat with long-sleeves. A solid front-back closing lab gown provides better protection to personal clothing than a traditional lab coat. If a higher degree of risk is involved then you should consider using a disposable gown. 3. Adjust the seating position for optimal operator comfort. It is recommended to use a height adjustable stool. Refer to section 3.5 on ergonomics for further information. 4. Fully open the sash window. Thoroughly surface-decontaminate the work surface, side walls, back wall, drain pan and internal surface of the window using 70% ethanol (or some other disinfectant depending on materials used in the BSC). Surface-decontaminate the UV lamp and electrical outlets as well. Do not use any disinfectant containing chlorine-based substances as this may cause corrosion of the stainless steel surfaces. 5. Surface-decontaminate all apparatus/items before loading them into the work zone. While loading the materials/ apparatus in the BSC, they should be arranged in such a way that the movement of contaminated items over the clean ones is minimized. This can be achieved by placing the clean items on say the left side of the BSC and the contaminated ones on the right side. Segregating the clean items from the contaminated ones in this way provides very good protection from cross-contamination. 6. Place all the apparatus/items on the safe working area as indicated on section 3.4.3 of User Section. 7. After all the apparatus/items have been arranged, adjust the sash to its normal operating height and allow the blower to run for another 3 minutes in order to purge work zone of contaminants that entered the work zone when the blower was off. 8. Minimize room activity (personnel movements, closing and opening of doors, etc.) since these external airflow disturbances may adversely affect the BSCs internal airflow, thereby possibly impairing the containment capabilities of the BSC.
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3.3.1 Turning on the BSC 1. Raise the sash to the indicated normal operational height (READY state). The lamp will turned on when this height is reached. 2. Turn on the fan by pressing the FAN button. Input the Fan PIN digit by digit when asked (default: 0001). This will also start the warm up procedure (default: 3 minutes). During the warm up procedure, all the buttons are not operational. 3. The BSC is ready for work. 3.4 Working in the BSC 1. Ensure that the sash is at normal operating height (READY state) before starting any experiment. If the alarm indicator lamp blinks and the alarm sound at the same time, discontinue work immediately and properly shutdown the BSC (Refer to section 3.6 for details on shutdown procedure). Thereafter you may refer to the troubleshooting section of this manual (SERVICE SECTION, CHAPTER 2). 2. Make sure that the front and back air grilles are not being obstructed by your arms or any other objects. 3. The safe working area of the BSC is the center flat portion of the tray that has no perforations.
Safe work area On AC2-L Divided Work Surface
4. As far as possible, start working from the side of the BSC where clean items/apparatus have been arranged and then move towards the sides where the contaminated/hazardous ones have been arranged. Such a practice would complement the technique of segregation of clean and dirty items/apparatus inside the BSC that has been discussed in section 3.3.5 in your endeavor to contain cross-contamination. Work with one specimen at a time and recap each specimen before moving to the next one. 5. Work as far inside the BSC as possible - at least 150 mm (6 in) behind the front air intake grille. Hold open tubes and bottles as vertically as possible to avoid spillage. Discard empty tubes and bottles into collection bags placed inside the BSC immediately after use. 6. Use convenient pipetting aids. Do not mouth-pipette. Use horizontal pipette discard pans containing some suitable disinfectant inside the biohazard safety BSC. Do not use vertical pipette discard canisters on the floor outside the BSC. Biohazard collection bags should be placed inside the BSC. 7. If streaking loops are to be sterilized to prevent cross-contamination of biological materials, an electrical incinerator is recommended. The use of Bunsen burner inside the work zone is not recommended. Use of Bunsen burners within Esco biosafety BSCs is entirely at your own risk and Esco accepts no responsibility or liability for their use. Bunsen burner flames can disrupt the laminar airflow and will contribute to heat build up within the BSC. However, if the use of a Bunsen burner is approved by a safety officer, it should be operated on the right side of the work zone. In Esco BSCs, the airflow sensor, when installed in the work zone, is mounted on the left side. Therefore operating the burner on the right side would not affect the BSC airflow monitoring system. All gas installations must be carried out in accordance with current national, state and local safety regulations by an authorized and approved installation engineer.
8. Surface-decontaminate before removing any potentially contaminated items from inside the BSC. 9. Work in the BSC in a slow and controlled manner. While putting items inside/removing items from the work zone, move your hands in and out of the work zone opening slowly and in a direction perpendicular to the plane of the work zone opening. Rapid movement of arms in a sweeping motion may disrupt the air barrier, thereby allowing contaminants to escape out of the BSC. Make sure that you surface decontaminate before removing arms from the BSC. 10. Place aerosol-generating instruments (if any) as far inside the BSC as possible 11. Clean items/materials should be at least 150 mm away from aerosol generating instruments/objects to minimize the chances for cross contamination. 12. Hold lids/covers over dishes/sample plates in order to prevent the downward air from impinging on the samples. 13. Place air turbulence generating equipments (if any) such as centrifuges, blenders or sonicators towards the back of the BSC. Stop other work while any of this equipment (if used) is in operation. 14. If a vacuum line is present, protect the building vacuum system from biohazards by placing a cartridge filter between the vacuum trap and the source valve in the BSC. 15. When the sash is fully closed, the blower should be turned off to prevent overheating. NB: Fan PIN or Admin. PIN would be needed for this. This is a safety feature to prevent unauthorized personnel from shu ting down the BSC. 3.4.1 Handling Spillage 1. Discontinue work process if possible. 2. Inspect spill. If contents of spill are potentially harmful, wear appropriate protective personal equipment. 3. Remove debris from spill and wipe up spills carefully to prevent further contamination. 4. Use absorbent pads or towels to cover the spill and then soak it with 10% bleach. Wait for 10 to 15 minutes before cleaning the spill and the area (and equipments) surrounding it using a suitable disinfectant. Generally a 1% solution of an iodophor decontaminant (Wescodyne or equivalent) is effective against most viruses, fungi, vegetative bacteria and non-encysted amoeba. 5. After the spillage has been cleaned, discard your outer gloves and start using a new pair. Allow the BSC to purge contaminants that might have been left behind in the work zone for 3 to 5 minutes. Autoclave all contaminated materials (including gloves, cloth, and sponge). Decontamination may be necessary if a spillage has possibly contaminated the inaccessible surfaces of the BSC 3.4.2 Alarms and Warnings BSC uses alarms to indicate that the condition inside the BSC is not safe for the operator, so check the LCD display to understand the cause of these alarms. The most common alarm is the SASH ALARM that indicates that the sash is neither at the normal operating height nor at fully closed position (UV mode) this condition can easily be corrected by putting the sash at the appropriate operation position. Another warning that should be acted upon is AIR FAIL! which indicates that there is airflow failure. The operator should check if there is any obstruction to the airflow, and correct it if possible. However, if the problem continues, the operator should stop working as the BSCs protection may have been compromised. Call service or Escos local distributor. After some time, the message on the display may change to SYS AIR FAIL! or RAND AIR FAIL!. These messages indicate whether the airflow failure occurs continuously or irregularly. There is no special procedure required for these messages. Please follow the procedure explained above for the standard AIR FAIL! message. Other alarms that indicate a failure or an error in the BSC system: ERR.AIRFAIL will be displayed if the blower is turned off while there is an airflow failure.
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ERR.MSWITCH will be displayed if the microprocessor (controller) detects more than one microswitch activated at the same time, which is impossible, as the sash can only be at one position at one time. This indicates a failure in the sash detection system. ERR.CALIB will be displayed if the airflow velocity sensor is not yet calibrated. 3.5 Working Ergonomics On most occasions, you would most likely be operating the BSC in sitting rather than standing posture. There are some obvious advantages of the sitting posture: The physiological energy cost and fatigue involved in sitting are relatively less Sitting posture provides the body with a stable support However, sitting position has some drawbacks too: The working area available is fairly limited There is a potential risk of being constrained in the same posture for a long time Sitting posture is one of the most stressful postures for ones back Therefore you should pay careful attention to the following guidelines in order to achieve comfortable and healthy working conditions: 1. Always ensure that your legs have enough legroom. 2. Keep your lower back comfortably supported by your chair. Adjust the chair or use a pillow behind your back whenever necessary. 3. You should place your feet flat on the floor or on a footrest. Dont dangle your feet and compress your thighs. 4. You should keep varying your sitting position throughout the day at regular intervals so that you are never in the same posture for too long. 5. Observe the following precautions with respect to your eyes: Give your eyes frequent breaks. Periodically look away from the work area and focus at a distant point. Keep your glasses clean. 6. Arrange the items/apparatus frequently used in your work in such a way that you can minimize the physical strain involved in handling them. 7. Exercise regularly The BSCs noise emission has been tested and found to be in compliance with EN 12469, ISO 4871 and NSF/ANSI 49 which is important to ensure health and comfort for the operator. Ergonomics accessories available with Esco include: Armrest padding Lab chair Footrest Please contact your local distributor or Esco for more information.
3.6 Cleaning and Shutting Down the BSC 1. As far as possible, it is recommended that the BSC be operated continuously in order to achieve optimal containment and cleanliness. NB: Airflow studies have shown that once the fan has been switched off, air from the BSC may escape due to the thermal currents from inside the BSC. So if you choose to turn off the BSC, you need to carefully adhere to the following guidelines so as NOT to compromise safety and containment during shutdown.
2. Biohazard waste should be discarded into biohazard bags (including the outer gloves, provided double gloving was employed by the operator). Seal the biohazard bags and dispose them off properly. If necessary, biohazard waste should be autoclaved. 3. Surface-decontaminate all the items/apparatus using 70% Isopropyl Alcohol (IPA) before removing them from the BSC. 100% IPA is not recommended because it will evaporate too fast before having sufficient contact time. It is recommended that the fan be left ON during this period. 4. Thoroughly wipe the work surface, side walls, inner back wall and drain pan using water and some mild antibacterial detergent. Any disinfectant containing chlorine-based substances may corrode the steel used in the BSC. So if such disinfectant is used, the residue must be immediately wiped off by using cloth and non-corroding disinfectant, such as 70% Isopropyl Alcohol (IPA). Periodically clean behind the sash window too. For this you will have to unscrew the sash window track by unfastening the bolts at both ends of the sash track. After that, lift the sash window. 5. Wipe off the detergent using water. Make sure that there are no traces of detergent left. 6. Wipe the work surface, side walls, back wall and drain pan again with 70% Isopropyl Alcohol (IPA) or any other disinfecting agents listed on the next page. 7. Leave the blower on for 3 minutes in order to purge airborne contamination from the work zone. (Default postpurge cycle time is 0 minute; this setting can be changed though - refer to section 3.7.3.3) 8. Remove the laboratory coat and thoroughly wash your hands using germicidal soap. 3.6.1 Turning off the BSC 1. Turn off the fan by pressing the FAN button. Input the Fan PIN digit by digit when asked (default: 0001). This will also start the post purge procedure (default: 0 minute). During the post purge period, all the buttons are not operational. 2. Lower the sash to the fully closed position (the display will show UV MODE). The sash can be lowered immediately after turning off the fan as it will not interrupt the post purge procedure. 3. Turn on the UV lamp (when present) to decontaminate the work area by pressing the UV button. Leave the UV lamp on for around 60 minutes to make sure the decontamination is done effectively this can easily be done by setting the UV timer (default: 0 minute). The UV lamp can only be turned on after the post purge procedure is finished. 3.6.2 Using UV Lamp (When Present) The UV light is a very effective germicide and virucide. Even at the minimum acceptable irradiance in a BSC 40 W/ cm2 (US Department of Health and Human Services et. al., 2000), it would only take 12.5 minutes to reach 30,000 W/ cm2 (1 W = 1 J/sec), which has been listed as germicidal for spore forming organism. Unlike many other type of decontamination agent, UV light doesnt leave any residue. The decontamination action stops upon de-energizing of the lamp. However, due to the short wavelength, the UV light does not penetrate well. Thus UV light can only be used to effectively disinfect the work area of an empty BSC. For any container stored inside the BSCs work area, the UV radiation will only disinfect the outer surface of the material, leaving the inner surface and the content inside the material untouched. UV light decontamination method may be used before and after working with vegetative organisms and viruses. However, it should not be the sole decontamination agent; the use of chemical decontamination agent is still encouraged.
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Minimize the material inside the BSCs work area during the process of UV light decontamination. A direct interaction with UV light can degenerate plastic- or rubber-based material and can cause other hazard. Make sure that the BSC sash is in the fully closed position and the interlock is working properly before activating the UV lamp. Avoid direct contact with skin and eyes as UV light is classified as a probable human carcinogen. The UV lamp should be activated for around 60 minutes to work effectively. Use the UV timer feature to easily control the period of decontamination. UV timer is disabled by default. Please refer to section 3.7.3.4 for information on setting the UV timer. Leaving the UV lamp on for over 60 minutes or even overnight is not recommended because it shortens the lifespan of the lamp. The UV lamps used in Esco BSC have a lifespan of 8,000 hours. The UV lamp should be cleaned of any dust and dirt weekly and changed annually to ensure its effectiveness. The use of UV lamp in BSC has been explicitly discouraged in all major international standards and recommendations. 3.6.3 Disinfecting Agents For stainless steel surfaces, all common disinfecting agents except chlorine-based ones are suitable. For powder coated surfaces, all common disinfecting agents are suitable. However, the BSC has been specifically evaluated for use with the following: 1N hydrochloric acid 1N sodium hydroxide 1% quaternary ammonium compound 5% formaldehyde 5,000 ppm hypochlorite 2% iodophor 5% phenol 70% ethyl alcohol
Depending on the contaminant involved at the time of operating the BSC, there are various other types of disinfecting agents that may be used. Table below outlines the effectiveness of various disinfecting agents against the different types of contaminants.
Quaternary Ammonium Compounds
Low Level
Decontaminant
Gluteraldehyde
Peroxide/ Peracetic acid/ Acetic acid

Sterilant
Chlorine Dioxide
Chlorine
Iodophor
Alcohol
Phenolic
Classification Parameters for use: Concentration Contact time (min.) Stability > 1 week (1) Agents: Bacterial Endospores Naked Viruses Mycobacterium Vegetative Bacteria Enveloped Viruses
Sterilant
Sterilant
High Level
Intermediate
Intermediate
Intermediate
2% 10-600 +
1% 10-720
1:5:1/1001000 ppm 10-600 +
0.01-5% 10-30
0.5-2.5% 10-30 +
70-85% 10-30 +
0.2-3% 10-30 +
0.1-2% 10-30 +
+ + + + +
+ + + + +
+ + + + +
+/+ + + + +/- (2) + + + +/- (2) + + + +/- (2) + + + + +
Characteristics: Inactivated by Organics Residual Corrosive Flammable Skin Irritant Eye Irritant Respiratory Irritant Toxic + + + + + + + + + + + + + + + + + + + + + + + + + + + + + +/+ + + + + + + +/+ + + +/+ + +
Use in BSCs: Routine Surface Decon Biohazardous Spill +/+ +/+/+ + + + + +/-
(1) Protected from light and air (2) Results vary depending on the virus In any of the following eventualities, the user should ensure that the BSC has been properly decontaminated, keeping in mind the nature of the pathogens used: At the time of moving/relocating the BSC At the time of changing the type of work being carried out in the BSC Before accessing contaminated areas for servicing, for example - filter replacement Decontamination Decontamination may frequently be carried out by means of formaldehyde fumigation or using other decontamination agents, such as chlorine dioxide or hydrogen peroxide. Decontamination is typically only done before filter or blower replacement. Decontamination process should only be carried out by trained personnel. For more information please refer to Chapter 1.9 of Service Section.
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3.7 Menu Options

When you are entering menu options, the alarm will sound to indicate that the microprocessor is not monitoring the operation of the BSC and as such will not give airflow alarms. No further warnings will be given. Therefore, it is highly recommended that the user set the ADMIN PIN, which will restrict unauthorized access to the menu. Please refer to section 3.7.3.1 for further details. The default ADMIN PIN from the factory is 0009.
Please refer to the following diagram for complete reference to all menu options available. Press UP or DOWN button to move through the menu options. Exit menu options after making any changes in order to prevent unauthorized access to the menu.
SET TIME WARM UP TIME POSTPURGE TIME SETTINGS UV TIMER AIRFLOW UNIT TEMPERATURE UNIT
SET CONSTANT CALIBRATION ZERO SENSOR CALIB SENSOR
CLEANING MODE
ENTER MENU
NEW ADMIN PIN NEW FAN PIN AIRFLOW METER ADMIN SETTINGS RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT
NORMAL MODE SET MODE MAINTENANCE MODE
3.7.1 Settings The user may use the settings menu function to customize the operation of the BSC to meet specific application requirements. 3.7.1.1 Set Clock (Time) Users can set the time by increasing/decreasing the hour and minute values. The correct time will be maintained even after the unit is turned off. Step
1 2 3 4 5
Action
To enter the menu, press MENU button. There will be a choice to either to enter cleaning mode or menu. Using the / and SET buttons choose ENTER MENU. Using the / and SET buttons to put in the Admin. PIN digit by digit. (Default: 0009) The alarm will sound. Wait until the alarm is disabled and the display shows SETTINGS. Using the / buttons, select SETTINGS from the menu and press SET to enter the Settings Menu.
Press
Display Shows
MENU SET SET

CLEANING MODE ENTER MENU PASSWORD: XXXX WARNING! ALARMS DISABLED
SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Set 24 Hour Clock HH:MM Format: hh:mm Set 24 Hour Clock HH:MM Format: hh:mm Set 24 Hour Clock HH:MM Format: hh:mm TIME SET
Using the / buttons, select SET TIME from the menu and press SET to set the time.
SET
7 8 9 The hour (hh) will flash, use the / buttons to select the correct hour and press SET to move on. The minutes (mm) will flash, use the / buttons to select the correct minutes and press SET to move on. The display will show TIME SET for 2 seconds.
SET SET
10
Exit the menu by pressing MENU button twice
MENU
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3.7.1.2 Warm Up Timer There will be a period of warm-up, before the fan is fully functioning. This is to ensure that the sensors, the fan, and the control system are stabilized, as well as purging the work zone of contaminants. The default setting is 3 minutes and user can set it up to 15 minutes. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Set Fan Warm Up Time XX Minutes Set Fan Warm Up Time XX Minutes WARM UP TIME SET
Using the / buttons, select WARM UP from the menu and press SET to set the warm-up time.
SET
7 The screen will show the number of minutes for the warm up timer. Use the / buttons to select the value you want. Press SET to move on. The display will show WARM UP TIME SET for 2 seconds.
SET
MENU
3.7.1.3 Post Purge Timer After the user switches off the fan, there will be a period of post-purge. This feature is to ensure that all residual contaminants are purged from the work zone. The default setting is zero minute and user can set it from zero minute to 15 minutes. Setting it to zero minute will disable this feature. However, it is recommended to purge the BSC by leaving the fan on for around 3 minutes after the work is complete. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Set PosrPurge Time XX Minutes Set Fan Warm Up Time XX Minutes WARM UP TIME SET
Using the / buttons, select POSTPURGE TIME from the menu and press SET to set the post purge time.
SET
7 The screen will show the number of minutes for the post purge timer. Use the / buttons to select the value you want. Press SET to move on. The display will show WARM UP TIME SET for 2 seconds.
SET
MENU
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3.7.1.4 UV Timer To switch off the UV lamp automatically after a fixed period, UV TIMER menu can be used. The UV TIMER can be set up to 18 hours. By default, this feature is disabled. UV lamp, then, has to be switched off manually. The most effective time for UV decontamination is around 60 minutes. Esco does not recommend that you leave the UV lamp on for more than 60 minutes or overnight as it shortens the lifespan of the UV lamp. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Set UV Timer HH:MM Format: hh:mm Set UV Timer HH:MM Format: hh:mm Set UV Timer HH:MM Format: hh:mm TIME SET
Using the / buttons, select UV TIMER from the menu and press SET to set the UV timer.
SET
7 8 9 The hour (hh) will flash, use the / buttons to select the correct hour and press SET to move on. The minutes (mm) will flash, use the / buttons to select the correct minutes and press SET to move on. The display will show TIME SET for 2 seconds.
SET SET
10
MENU
3.7.1.5 Airflow Unit Selection Using this option, you can select the unit in which air velocity is measured and displayed. You can choose between meter per second (m/s) and feet per minute (fpm). When the fan is running and also during the calibration process, airflow measurement is displayed in the selected unit.
Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Feet per minute Meter per second
Using the / buttons, select WARM UP from the menu and press SET to set the warm-up time.
SET
7 8 Using the / buttons, select from the available options and then press SET to record your choice. Exit the menu by pressing MENU button twice
SET MENU
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3.7.1.6 Temperature Unit Selection Using this option, you can select the unit in which temperature is measured and displayed. You can choose between Celsius and Fahrenheit. The exhaust temperature is displayed in the selected unit. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE SET TIME WARM UP TIME POSTPURGE TIME UV TIMER AIRFLOW UNIT TEMPERATURE UNIT Celcius Fahrenheit
Using the / buttons, select TEMPERATURE UNIT from the menu and press SET to select the display unit for cabinet temperature.
SET
7 8 Using the / buttons, select from the available options and then press SET to record your choice. Exit the menu by pressing MENU button twice
SET MENU
3.7.2 Calibration The purpose of calibration is to ensure the accuracy of the airflow display and alarm (if present). This involves measuring airflow with reference instrumentation and establishing reference between airflow sensor(s) on the BSC to the standard reference. Calibration should only be carried out by trained personnel. This section is presents a brief overview of the calibration menu function. For more information, refer to the service section of this User and Service Manual. 3.7.2.1 Set Constant Every sensor manufactured by Esco has a specific Sensor Constant which is used for temperature compensation performed by the temperature sensor. 3.7.2.2 Zero Sensor This option is to let the controller record the specific sensor output voltage and correspond it to 0 m/s or 0 fpm. 3.7.2.3 Calib Sensor This option allows proper calibration and operation of the airflow sensor alarm. There will be three points to be calibrated, namely inflow fail point, inflow nominal point, and downflow nominal point.
3.7.3 Admin The admin menu allows you to change both fan and Admin. PIN, also to disable it (not recommended). The reset blower hour meter and reset UV hour meter functions are usually used after you change the blower or UV lamp as they can easily gives you the indication on when to do BSC maintenance. While the reset default function will return the options in the settings menu to their factory settings. 3.7.3.1 New Admin. PIN Admin. PIN restricts access to MENU functions, including service functions, like calibration. User must enter four digits PIN before accessing MENU. Admin. PIN has higher priority and can be used to control the fan (override Fan PIN). Admin. PIN can also be used to switch to maintenance mode from ERR.MSWITCH and AIR FAIL! error condition. The default PIN is 0009. Setting PIN to 0000 will disable this feature. Please follow the following steps to set a new Admin. PIN: Step
1 2 3 4 5
Action
To enter the menu, press MENU button. There will be a choice to either to enter cleaning mode or menu. Using the / and SET buttons choose ENTER MENU. Using the / and SET buttons to put in the Admin. PIN digit by digit. (Default: 0009) The alarm will sound. Wait until the alarm is disabled and the display shows SETTINGS. Using the / buttons, select ADMIN SETTINGS from the menu and press SET to enter the Settings Menu.
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT Set New Admin PIN XXXX Format: x Set New Admin PIN XXXX Format: xx Set New Admin PIN XXXX Format: xxx Set New Admin PIN XXXX Format: xxxx Set New Admin PIN XXXX Format: xxxx NEW ADMIN PIN SET
Using the / buttons, select NEW ADMIN PIN from the menu and press SET to set a new administrator password.
SET
7 8 9 10 11 Using the / buttons, select the first numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the second numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the third numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the last numerical digit of the PIN number and then press SET to move on. The display will show NEW ADMIN PIN SET for 2 seconds and then return to the ADMIN SETTINGS menu.
SET SET SET SET
12
MENU
: 33
3.7.3.2 New Fan PIN Fan PIN restricts access to fan control. User must enter four-digit PIN before switching fan on or off. As such, it can restrict access to operating the BSC by unauthorized personnel. It will also prevent unauthorized shutdown of the BSC when continuous operation is required. Note that continuous operation is recommended for better safety. Fan PIN is also needed to disable the alarm when the sash is fully raised and cleaning needs to be performed. It is recommended that the Fan PIN be issued only to personnel authorized to use the BSC. The default PIN is 0001. Setting the PIN to 0000 will disable this feature. Please follow the following steps to set a new Fan PIN:
Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT Set New Fan PIN XXXX Format: x Set New Fan PIN XXXX Format: xx Set New Fan PIN XXXX Format: xxx Set New Fan PIN XXXX Format: xxxx Set New Fan PIN XXXX Format: xxxx NEW ADMIN PIN SET
Using the / buttons, select NEW FAN PIN from the menu and press SET to set a new administrator password.
SET
7 8 9 10 11 Using the / buttons, select the first numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the second numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the third numerical digit of the PIN number and then press SET to move on. Using the / buttons, select the last numerical digit of the PIN number and then press SET to move on. The display will show NEW ADMIN PIN SET for 2 seconds and then return to the ADMIN SETTINGS menu.
SET SET SET SET
12
MENU
3.7.3.3 A/F Monitor Whenever the air velocity falls below the fail point, the air fail alarm will be triggered. This option is used to enable/ disable alarm. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT DISABLE ENABLE
Using the / buttons, select AIRFLOW MONITOR from the menu and press SET to enable or disable the airflow monitor (activates/ deactivates alarms).
SET SET MENU
The screen prompts you with a warning to make sure that you understand this process. Press the SET button to confirm that you want to reset the meter. To cancel, press MENU button. Exit the menu by pressing MENU button twice
: 35
3.7.3.4 Reset Blower Hour Meter This option is used to reset the blower hour meter. The blower hour meter indicates how long the blower has been in operation. Maximum counter is set at 9999 hours. The counter value can be checked while in maintenance mode. The value can also provide some help in setting up maintenance schedule, including filter change. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT Reset Fan Hour Meter Read Manual!!! And Press SET Button
Using the / buttons, select RESET B/H/M METER from the menu and press SET to reset the blower hour meter.
SET
7 The screen prompts you with a warning to make sure that you understand this process. Press the SET button to confirm that you want to reset the meter. To cancel, press MENU button.
SET or MENU MENU
3.7.3.5 Reset UV Hour Meter This option is used to reset the UV hour meter. The UV hour meter indicates how long the UV lamp has been used. Maximum counter is set at 9999 hours. The counter value can be checked while in maintenance mode. Please reset the UV hour meter after each UV replacement.
Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT Reset UV Hour Meter Read Manual!!! And Press SET Button
Using the / buttons, select RESET UV/H/M METER from the menu and press SET to reset the blower hour meter.
SET
SET or MENU MENU
: 37
3.7.3.6 Reset Default User can reset the default setting by choosing this option. The features being reset are warm-up period (3 minutes), post-purge period (0 minute), UV timer (0 minute), airflow unit (Metric), temperature unit (Celsius), Admin. PIN (0009), and Fan PIN (0001). Note that the calibration settings cannot be reset as it may cause the BSC to operate in an unsafe manner. The hour meters cannot be reset either. Step
1 2 3 4 5
Action
Press
Display Shows
MENU SET SET

SET
SETTINGS CALIBRATION ADMIN SETTINGS SET MODE NEW ADMIN PIN NEW FAN PIN AIRFLOW MONITOR RESET B/H/M METER RESET UV/H/M METER RESET DEFAULT Reset Default Values Read Manual!!! And Press SET Button
Using the / buttons, select RESET DEFAULT from the menu and press SET to reset the blower hour meter.
SET
SET or MENU MENU
3.7.4 Setting the Mode BSC has two working mode, the default normal mode which is used in a day to day activity, and maintenance mode. 3.7.4.1 Normal Mode Every time the BSC is restarted, this mode will be activated by default. In this mode, all alarms and interlocks are enabled. 3.7.4.2 Maintenance Mode Maintenance mode should only be accessed by qualified personnel during maintenance. In this mode, all alarms are disabled and all interlocks are defeated.
User Section Chapter 4 Maintaining Your BSC
: 39
CHAPTER 4 Maintaining Your BSC

Proper and timely maintenance is crucial for trouble free functioning of any device and your Esco BSC is no exception to this rule. We strongly recommend that you follow the maintenance schedule suggested hereunder in order to obtain optimal performance from your Esco BSC. Description of Task to Perform
1. Surface decontaminate the work zone 2. BSC power-up alarm verification 3. Thoroughly surface decontaminate the drain pan 4. Check the paper catch for retained materials 5. Clean the exterior surfaces of the BSC 6. Clean the sash window using cleaning mode 7. Check all service fixtures (where present) for proper operation 8. Inspect the BSC for any physical abnormalities or malfunction 9. Clean up stainless steel surfaces with MEK 10. Re-certification 11. Change UV Lamp (where present) 12. Change the fluorescent lamps
Maintenance to be carried out every

Day Week Month Quarter 1 Year 2 Years
1. Surface decontaminate the work zone. Thoroughly wipe the work surface, side walls, inner back walls and all other surfaces in the interior of the BSC using a disinfectant appropriate for the work being conducted in your lab. If using a commercial preparation, it is helpful to follow up your wipe with a 70% ethanol rinse. Ethanol can help the disinfectant to evaporate cleanly. Do not use any disinfectant containing chlorine-based substances as it may corrode the steel used in the BSC resulting in irreparable damage to the BSCs structure. Surface decontamination should be carried out for the UV lights and electrical outlet as well. While cleaning the interior of the BSC, make sure that no parts of your body except your hands go inside the BSC. Please refer to the user section for details of appropriate disinfecting agents.
2. BSC power-up alarm verification. When the BSC is powered up, the LCD screen will show PLS CHECK PANEL, all LEDS on the Sentinel control panel will be illuminated and the alarm will sound. This state should last 6 seconds while the system initializes. Upon correct initialization the alarms will silence, LEDS will assume normal state and the LCD screen will show the system status.
3. Thoroughly surface-decontaminate the drain pan. Thoroughly wipe the drain pan using a disinfectant appropriate for the work being conducted in your lab. If using a commercial preparation, it is helpful to follow up your wipe with a 70% ethanol rinse. Ethanol can help the disinfectant to evaporate cleanly. Do not use any disinfectant containing chlorine-based substances as it may corrode the steel used in the BSC resulting in irreparable damage to the BSCs structure. Please refer to the user section for details of appropriate disinfecting agents.
40 : User Section Chapter 4 Maintaining Your BSC
4. Paper catch The purpose of accessing the paper catch is to remove any retained materials that might cause obstructions. Care must be taken as the area is contaminated. Before opening the paper catch: Use appropriate personal protective equipment (PPE). Wipe down the interior of the BSC including the top and bottom surfaces of the work tray(s) while airflow is operating. If the interior cannot be wiped down, the BSC should be decontaminated. Accessing the paper catch 1. Turn the blower off. Then raise the sash to fully open position for easy access. 2. Remove the tray(s) to access the paper catch. 3. The figure beside, points to the location of the paper catch. Locate and remove any material trapped. Be careful of any sharp objects that might be present. You might want to use a mirror to help you see better.
1
3 2
5. Clean the exterior surfaces of the BSC. Using a damp cloth, clean the exterior surfaces of the BSC, particularly the front and top of the BSC in order to remove the dust accumulated there. 6. Clean the sash window using cleaning mode. The main purpose of this process is to clean the top part of the sash from any dust or decontaminant. 1. Remove armrest 2. Press MENU button and select CLEANING MODE - this mode allows you to lower the sash further than usual 3. Lower the sash by pressing the DOWN button continuously until the sash is in the lowest position and create a 10 cm opening between the sash and the diffuser 4. Open the front panel.
Armrest
Diffuser Sash window
5. Slide your hand through the opening and clean the inner side of the sash using cloth and IPA 70% 6. After cleaning, push the UP button continuously until the sash is in the sash ready position 7. Close the front panel. 8. Leave the Cleaning Mode by pressing MENU button 9. Reattach armrest
User Section Chapter 4 Maintaining Your BSC
: 41
7. Check all service fixtures (where present) for proper operation. Observe local, state and national regulations as to when you need to get your service lines and fixtures certified. Check both fluorescent tubes (and UV light if present) to ensure that they are functioning properly and replace the bulbs if necessary. For removing stubborn stains or spots on the stainless steel surface, make use of MEK (Methyl-Ethyl-Ketone). In such cases, make sure that you wash the steel surface immediately afterwards with clean water and some liquid detergent. Use a polyurethane cloth or sponge for washing. Regularly cleaning the stainless steel surface can help you retain the attractive factory finish. All BSCs must be re-certified annually by a certified engineer. The engineer will test down flow and inflow velocities, air flow patterns, the filter and perform a BSC leak test to ensure that the BSC is working properly. To remove the old lamp, turn the tube 90 degrees and slide it out from the bracket. To insert the new lamp, slide it in to the socket and when fully inserted, turn the bulb 90 degrees to lock into position. Surface decontamination should be performed after changing the UV bulb. There are two or four fluorescent lamps in the BSC; they are located behind the blue panel at the top of the sash. Before changing the fluorescent bulbs, ensure that the BSC is powered down and disconnected from the electrical supply. Raise the front cover and locate the bulbs. Remove the power clips at the ends of the bulbs by gently pulling whilst holding the bulb steady. Remove the bulbs from the mounting clips and replace with new ones. Replace the power clips on the ends of the new bulbs and ensure they are firmly seated. Close the panel, reconnect the BSC to the electrical supply and test the bulbs for proper operation. 8. Inspect the BSC for any physical abnormalities or malfunction.
9. Clean up stainless steel surfaces with MEK
10. Re-certification
11. Change UV lamp (where present).
12. Change the fluorescent lamps.
Log Record It is good practice (and in some cases regulatory requirement) to maintain a log of all maintenance work carried out on your BSC. Esco has an example Maintenance Log available for download from the resources section of our website,
SERVICE SECTION
Service Section Chapter 1 Re-Certification and Maintenance by Service Personnel
: 45
CHAPTER 1 Re-Certification and Maintenance by Service Personnel

Esco products generally provide years of trouble-free operation however like all equipment they require maintenance and service. Maintenance and service should be carried out by trained personnel. Esco offers training courses to equip service providers with the latest skills, information and tools to successfully maintain and service Esco products. For more information on the nearest training course, please contact Esco. Service providers should familiarize themselves with the basic operating principles of products before working on them. Suitable references include information in the User Section of this Manual or brochures which may be downloaded from the Esco web site. Biological safety cabinet (BSC) generally require: - Re-certification, when: a. The BSC is re-located b. BSC performance is suspected c. After filter or blower replacement d. At least once a year During recertification: a. BSC airflow velocities and flow patterns are verified against the manufacturers specifications and relevant international standards b. Filters are scan-tested to ensure they do not leak c. Operator comfort tests may be performed d. If airflow velocities are found to be off setpoint, adjustments are made as part of the certification process before final values are recorded. - Airflow alarm calibration, when: a. The cause of the airflow alarm cannot be determined b. Re-certification indicates the displayed airflow deviates by >0.02m/s (4fpm) from actual measured velocities. (Only for products with airflow velocity displays) - Decontamination before filter or fan replacement, or as specified. - Filter replacement, when: a. The filters are clogged and the fan(s) are already adjusted to maximum setting b. Filter leaks which cannot be repaired are found during scan-testing (Before filter replacement, the BSC must be decontaminated.)
- Fan replacement (typically rare) if failure occurs. - Routine maintenance also includes: a. Fluorescent lamp(s) replacement - typically once every 2 years. b. UV lamp replacement - typically once every 1 year.
46 : Service Section Chapter 1 Re-Certification and Maintenance by Service Personnel
1.1 Certification Flowchart
Decontamination
Motor Voltage Checking
Filter Leak Test
Microprocessor re-calibration (As needed)
Smoke Pattern
Noise Level
Vibration
Light
For new cabinets / commissioning For used / contaminated cabinets Mandatory Optional
Note: Motor Voltage Checking is only done for new cabinets / commissioning.
1.2 Esco Accuflow G2 Speed Controller

Lock LED Adjust LED
AC Line Voltage LED
Adjust/Lock Switch
Up/Down Button Frequency LED
Remote Operation LED
Esco Accuflow G2 is an AC motor speed controller than can adjust and maintain the speed of an AC motor by maintaining AC average motor voltage at a preset level. It automatically compensate for any fluctuations in the AC power line voltage to maintain a stable AC motor voltage output. Accuflow G2 is designed to bring the motor voltage back to within 0.5 V from the pre-set level for 10% fluctuation in AC line voltage.
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Accuflow G2 requires the ambient temperature range of 5 35o C (when Accuflow G2 is placed inside a cabinet) for operation. Accuflow G2 can operate at various AC line voltages and frequencies worldwide (230/115 V AC at 50/60 Hz). It measures the line voltage and frequency automatically upon every power up and shows the AC line condition with the LED indicators accordingly. Accuflow G2 is capable of memorizing up to 4 pre-set speed settings value at 4 different AC line combinations. 1.2.1 Accuflow G2 Position In a unit, Accuflow G2 is located inside the electrical panel.
1. Unscrew the two pan-head screws that locked the front panel. 2. Open the front panel 3. Unscrew the two thumb screws that locked the electrical panel 4. Open the electrical panel 5. The Accuflow G2 is located on the cover of the elctrical panel Electrical panel cover Accuflow G2 Front panel
1.2.2 Accuflow G2 Speed Setting To set the speed setting: 1. Move the LOCK/ADJUST switch to ADJUST position The ADJUST LED will light up. 2. Press the Increase or Decrease button to adjust the voltages respectively. The output can be adjusted in a very small step (~ 0.1 V/step) by clicking the button, or larger steps (up to 2 V/step) if the button is pressed and hold for more than ~ 2 seconds. 3. Move the LOCK/ADJUST switch back to LOCK position when the adjustment is done. The LOCK LED will light up. This will record the adjusted output voltage level to Accuflow G2s memory automatically.
1.3 Unit Re-Certification Steps to perform certification: Turn Fan and Lights on Place Cabinet in Maintenance Mode In Maintenance Mode all alarms are defeated and the window can be moved without the light being switched off or alarms sounding. Procedure: Accessing Microprocessor Menu 1. Press MENU button. Enter the ADMIN PIN (Default ADMIN PIN is 0009) If SETTINGS is displayed the ADMIN code has been removed proceed to step 3 a. Press SET with 0 flashing b. Press SET with 00 last digit flashing c. Press SET with 000 last digit flashing d. Press SET with 0000 last digit flashing e. Press button to key in 9 f. Press SET
2. Alarm will sound, wait until alarm stops. Cabinet should display.
MENU OPTIONS XX:XX SETTINGS CALIBRATION ADMIN SETTINGS
3. Use the button until SET MODE. Press the SET button. 4. Use the button until MAINTENANCE. Press the SET button. The cabinet is in maintenance mode the LCD will continuously blink and (!) is displayed to indicate unsafe condition. 5. Press the MENU button to exit from the menu tree. 6. Open the RED electrical box on the inside of the front panel and locate the potentiometer positioned toward right side of electrical panel. 7. Connect an AVERAGING Volt Meter to the motor voltage sampling port, the blue and black wires, connected to a opened connector. 8. Set the ACCUFLOW to open loop position.
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Inflow Velocity Test (Cabinet should be on for 10 minutes before setting airflows) Direct Inflow Measurement (DIM) method (first inflow method) was performed using an 8 flow hood that measured the inlet volumetric flow rate on the front aperture at nominal operating speed. Procedure: 1. Remove arm rest. 2. Set the Flow Hood in the front opening and lower the window onto the hood. 3. Cover open area with paper and tape. 4. Take Five (5) readings compensating for Temperature, Pressure and Humidity: 1 2 3 4 5 Total Average (total divide by 5)
Avg. inflow rate (Vol.): _______________ l/s
Inflow velocity (v): Vol. / A = _______________ m/s
Acceptance: Inflow velocity must be 0.45 0.025 m/s Cabinet

Target Nominal Inflow Point (l/s) Target Nominal Inflow Point (cmh) Width (m) Sash Height (m) Aperture Area (A) (m2): width x height
AC2-4
99 356 1.270 0.173 0.220
AC2-6
146 524 1.870 0.173 0.324
5. Remove flow hood and replace arm rest
Secondary Inflow Measurement Method using ESCOs probe holder and gauge block: 1. Remove the arm rest 2. Cover the small gap between the front grill / work surface and the cabinet with tape. Mark the probe position on the tape, referring to chart below. Cabinet
Width (mm) Distance from walls (mm) Distance apart (mm)
AC2-4
1270 127 127
AC2-6
1870 134 134
3. Adjust the window to a 76 mm (3) opening Use the ESCO AC2 gauge block and lower the sash window until it consistently touches the window on the left and right side of the BSC. Remove the gauge block before taking airflow reading. 4. Mount the thermo-anemometer on the probe holder. Adjust the probe tip 38 mm (1.5 in) below the sash window lower edge in the same front-to-back plane as the window. It is very important that the orientation of the probe with respect to the sash glass be correct or the inflow measurement will not be accurate. The probe must be parallel to the sash glass angle and in the same plane as the sash glass. Adjust probe as required to maintain plane of window
5. Set the thermo-anemometer to 20 sec averaging function. Take the readings and record the inflow velocity below:
AC2-4__ (9 points) AC2-6__ (14 points) 1 2 3 4 5 6 7 8 9 10 11 12 13 14
6. Take the average of the readings. Multiply the average by the conversion factor which converts the velocity reading for the 3 in restricted window opening to normal window opening velocity. Cabinet
Conversion factor
AC2-4
0.530
AC2-6
0.484
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Example: If the average reading of a AC2-4 BSC at 76 mm opening is 0.85 m/s then the inflow reading at normal window opening is: 0.85 m/s x 0.530 = 0.45 m/s. Acceptance: Inflow velocity must be 0.45 +/- 0.025 m/s
Sash window lower edge Gauge block Masking tape covering the gap between tray and BSC with marks to indicate probe position Recommended Thermoanemometer: TSI Velocicalc Plus 8384 Probes white tip is 38 mm (1.5 in) from sash window lower edge
Probe holder with magnet attached to bottom of BSC and touching front nosing of BSC
Recommended instrument: TSI Inc. (USA) 8384 Thermo-Anemometer (auto temperature correction) Accuracy: 3% of reading or 3 m/s. Downflow Velocity Test 1. Remove the UV bulb and IV Rod 2. Use a calibrated thermo-anemometer on a plane that is 10 cm above the lower edge of the sash. The velocity reading should be compensated for Temperature, Pressure, and Humidity (Dew Point Temperature). Downflow Grid: Cabinet
Width (mm) Left to Right Front to Back Distance from walls (mm) Distance apart (mm) Distance from walls = Y1 (mm) Distance apart = Y (mm)
AC2-4__
1270 159 318 138 138
AC2-6__
1870 234 468 138 138
552 Y1 Y
Y1
1/8
1/4
1/4 Width 4, 6 ft Unit
1/4
1/8
Note: Probe Orientation for downflow velocity measurements: Front row - probe pointed to the front Middle and back row - probe pointed to the right side wall on the right half of the cabinet, and pointed to the left side wall on the left half If there is a reading located side-to-side in the center of the back or middle row the probe should be pointed toward the left side wall Average : _________ m/s
Max Deviation : _________ m/s = _________ % Acceptance Result : Average downflow velocity must be within +/- 0.025 m/s of 0.32 m/s (Escos setpoint) All individual downflow velocity readings must be within 20% from the average. : Pass / Fail
Balancing Inflow and Downflow (If Required) The proper inflow and downflow is achieved by adjusting the fan voltage of the cabinet as required The air flows have been set, and the BSC air flows are certified
Calibrating the Microprocessor (Not Required on New Cabinets) Calibrating the microprocessor establishes correlation between the sensor analog voltage output and the airflow measured with an independent instrumentation. This enables the microprocessor to display airflow velocities accurately. Calibration is required if the display does not correspond +/- 0.02 m/s with the instruments used to certify the cabinet. There are 6 steps: i. Setting the Sensor Constant ii. Setting the Zero Point iii. Obtaining the Motor Voltages for Nominal Inflow and Fail Inflow Set Point iv. Calibrating the Fail Inflow Set Point v. Calibrating the Nominal Inflow Set Point vi. Calibrating the Nominal Downflow Set Point Steps i and ii are required ONLY under the following conditions: 1. New unit at the factory. 2. New sensor, main PCB board, or software installed. 3. Erratic readings or the calibration is suspect due to earlier adjustments
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A. Sensor Constant The Sensor Constant has been set at the factory and should not need to be entered into the microprocessor. Every sensor manufactured by Esco has a specific Sensor Constant written on the sensor sticker. It is also recorded on the Factory Test report. This sensor constant is used for temperature compensation. Entering Sensor Constant: 1. Press MENU button. LCD would then show:
2. Use the button until the cursor is at CALIBRATION. Press the SET button. LCD would then show:
MENU OPTIONS XX:XX SET CONSTANT ZERO SENSOR CALIB SENSOR SETTINGS
3. Press SET button. LCD would then show:

MENU OPTIONS XX:XX CONSTANT-1:00 CONSTANT-2:00
At displays CONSTANT_1: 00. Number 00 is blinking. The default sensor constant is 0. Use the or button to the sensor constant in increment of 1. Press SET button to record the constant. At displays CONSTANT_2:00. Number 00 is blinking. The default sensor constant is 0. Use the or button to the sensor constant in increment of 1.
4. Press SET button. LCD would then show :

5. Press button to Set the Zero Point or Press the MENU button twice to exit from the menu tree. B. Setting the Zero Point - The Zero Point has been set at the factory and should not need to be entered into the microprocessor. 1. If ZERO SENSOR is displayed, Press SET. If Zero Sensor is not displayed, Press MENU button - Press the button until the LCD displays CALIBRATION.
2. Press the SET button and Use the button until the LCD displays ZERO SENSOR. Press SET. Note: The fan is automatically switched off for 3 minutes if it was on 3. The LCD would then show:
MENU OPTIONS XX:XX Set Airflow 0.00m/s Block Airflow Sensor And Press SET Button
Press SET- DO NOT Press SET until sensor is blocked.
4. Locate the exhaust sensor on the top of the unit and downflow sensor below the downflow diffuser. Look down through the holes in the top of the sensor box. Make sure the sensors holes are located inline with the air coming out of the filter. 5. Cover the downflow sensor and the openings on the top of the exhaust sensor box with tape. The sensor must be isolated from ambient air. 6. The LCD would then show:
MENU OPTIONS XX:XX Set Airflow 0.00m/s New ADC IF0: XXXX New ADC DF0: XXXX
The ADC values signify the airflow sensor output. Wait and observe these values until they stabilize and press the SET button to confirm the ZERO point
7. ZERO POINTS SET will be displayed for approximately 1 second. The LCD would then show:
MENU OPTIONS XX:XX Set Airflow 0.00m/s New ADC IF0: XXXX ZERO POINTS SET
8. Press the MENU button twice to exit from the menu tree. 9. REMOVE tape from sensor (Very important!) C. Obtaining the Correlating Motor Voltages to Nominal Inflow and Fail Inflow. You will find the motor voltages that correlate to the Nominal Inflow and Fail Inflow set points. This is a prelude to calibrating the inflow set points on the microprocessor. 1. If the cabinet airflow has not been properly balanced, place the Thermo-Anemometer in the work area to measure the downflow, then calculate the average downflow (Range: 0.32 +/- 0.025 m/s). Adjust the motor voltage as necessary to obtain this. 2. If the cabinet airflow has not been properly balanced, place the Flow Hood on the front of the cabinet to ensure correct Nominal Inflow (Range: 0.45 m/s +/- 0.025 m/s). Adjust the motor voltage as necessary to obtain this. 3. Connect an averaging Digital Volt Meter (DVM) to the motor voltage sampling port. 4. Record the Nominal Inflow Voltage on the table at step C.7 below. 5. Adjust the potentiometer R75 on the speed control board for the main to to obtain 0.40 m/s inflow determined by your Flow Hood. (Start by adjusting voltage to Fail Inflow Set Point recorded by factory or from the last certification report). Do not change the damper opening. Cabinet
Target Fail Inflow Point (l/s)
AC2-4
87
AC2-6
130
6. Record the Fail Inflow Voltage on the table at step C.7 below.
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7. This is the table containing the summary of cabinet setting: Cabinet Setting
Nominal Inflow Voltage (VAC) Fail Inflow Voltage (VAC) Nominal Downflow (m/s) Sensor Constant_1 Sensor Constant_2
Value
8. Remove the flow hood and put the arm rest back. D. Calibrating the Fail Inflow Set Point 0.40 m/s (inflow velocity) 1. Press the MENU button. LCD would then show
Use the button until the cursor is at CALIBRATION. Press the SET button. LCD would then show
3. Use the until the cursor is at CALIB SENSOR 4. Press the SET button. The LCD will display a warning as shown:
MENU OPTIONS XX:XX Calibrate Sensors Read Manual ! ! ! And Press SET Button
5. Press the SET button. The LCD will prompt for the calibration of the inflow minimum point as shown:
MENU OPTIONS XX:XX Set Inflow 0.40m/s New ADC IF1 : XXXX
Note: The fan is automatically switched on if it was off 6. Using Digital Volt Meter (DVM), ensure that the potentiometer R75 on the speed control board for the main fan is still at Fail Inflow Set Point of 0.40 m/s as recorded in Step C above. 7. Wait and observe the value of New ADC IF1 to stabilize and press the SET button.
E. Calibrating the Nominal Inflow Set Point 0.45 m/s (inflow velocity) 1. After calibrating the inflow minimum point, the LCD will prompt for the calibration of the inflow nominal point as shown:
MENU OPTIONS XX:XX Set Inflow 0.45m/s New ADC IF2: XXXX
2. Using Digital Volt Meter (DVM), adjust the potentiometer R75 on the speed control board for the main fan to the voltage for Nominal Inflow Set Point of 0.45 m/s as recorded in Step C above. 3. Wait and observe the value of New ADC IF2 to stabilize and press the SET button. F. Calibrating the Nominal Downflow Set Point 1. After the above recording is done, the LCD would prompt for the calibration of the downflow nominal velocity.
MENU OPTIONS XX:XX Measured Velocity Downflow 0.00m/s
2. Maintain potentiometer R75 on the speed control board for the main fan to voltage at Nominal Inflow Set Point. The number 00 is blinking and adjust the downflow display using or buttons to match the Thermo-Anemometer average downflow reading, in increments of 0.01 m/s. 3. Press SET After pressing set, the microprocessor will immediately record the downflow, without 3 minutes waiting time. 4. CALIBRATION DONE will be displayed for approximately 1 second, and the buzzer will confirm with a beep as shown below.
MENU OPTIONS XX:XX Measured Velocity Downflow : 0.32 m/s CALIBRATION DONE
5. LCD would then show

6. Press menu button twice to exit from menu tree. 7. Set the ACCUFLOW back to the closed loop position. Measure the nominal inflow when the supply voltage is varied for 10%. The change in the inflow has to be less than 0.025 m/s. Pass / Fail The Microprocessor has been calibrated
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HEPA/ ULPA Filter PAO Leak Test Procedure: 1. Remove the downflow diffuser. 2. Access the PAO sampling port. There are 2 possible scenarios: a. New / decontaminated cabinet: lift the work tray, and connect PAO sampling tube to photometer b. Contaminated cabinet: do not use the PAO sampling tube. Use the calculated method instead. 3. Place the aerosol generator on the right side of the work surface, because the blower is on the right side of the operator. Alternatively, use a T-shaped tube to distribute the aerosol evenly across the work zone. 4. Connect the aerosol generator to compressed air or compressed nitrogen supply. Open the aerosol generator valve(s). Set the supply pressure valve to 23 psi for PAO or 20 psi for DOP. 5. Turn on the aerosol photometer. Check the upstream aerosol concentration. See chart below: Record the concentration below: Minimum required
PAO DOP 7.5 g / liter 10 g / liter
Recommended
15 g / liter 20 g / liter Actual PAO concentration: ______ g/liter
If calculated method is used, along with ATI TDA-4Blite aerosol generator, then the upstream aerosol concentration can be calculated using the following equation: Aerosol concentration = 13,500 x N , where N = Number of Laskin nozzle(s) Total Airflow Cfm
See chart for total airflow below: Cabinet

Total Airflow (cfm)
AC2-4
651
AC2-6
966
6. Check background aerosol concentration in room. Make sure its not too high. Also check the upstream aerosol concentration again after the test is completed to ensure same value. 7. With traverse speed of 2 in/sec, scan the downflow and exhaust filter perimeter, and filter media for leakage and record the results below:
DOWNFLOW FILTER Leaks detected in media Leaks detected in gasket Particle penetration Yes / No Yes / No _________% EXHAUST FILTER Leaks detected in media Leaks detected in gasket Particle penetration Yes / No Yes / No _________%
Acceptance Result
: Maximum particle penetration of 0.01% for both filters; no filters leaks present. : Pass / Fail
8. Plug the sampling tube, wipe the surface exposed to DOP / PAO. Install the downflow diffuser.
Noise Level Test With a calibrated noise level meter obtain a noise level of the cabinet during normal operation, at 30 cm in front of the drain pan lower edge and 38 cm above the work surface area (not the front grill).
BSC noise level (dBA) Ambient noise - fan off (dBA) Corrected noise level (dBA)
Factory Acceptance: Maximum corrected noise level of 67 dbA (Based on NSF 49 standard). If the difference between cabinet and ambient noise is less than 10 dBA, a correction factor must be used. This noise result may be different than noise stated in brochures, which obtained from anechoic chamber. Result: Pass / Fail Vibration Test
Actual vibration (BSC on), averaged from 20 consecutive readings Background vibration (BSC off), averaged from 20 consecutive readings Net vibration (actual vibration minus background vibration) x 10-5 m RMS amplitude x 10-5 m RMS amplitude x 10-5 m RMS amplitude
Acceptance Result
: Maximum net vibration should not exceed 0.5 x 10-5 m RMS amplitude : Pass / Fail
Lighting Intensity Test Use a light intensity meter to obtain light levels at the cabinet work surface. Zero the meter before taking the readings. Readings are taken along the front to back centerline within the interior work area:
Back Wall 150 x x x x x x 150
Average Value:
Cabinet ON
Cabinets LIghts ON 240
Cabinet OFF
Cabinets LIghts OFF 4 ft Unit 6 ft Unit
Net: ON-OFF
All units are lux Cabinet

Width (mm) Left to Right Front to Back Distance from walls (mm) Distance apart (mm) Distance from walls = Y1 (mm) Distance apart = Y (mm)
AC2-4__
1270 159 318 138 138
AC2-6__
1870 234 468 138 138
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Airflow Smoke Patterns Test A suitable visible smoke generator was used to visualize cabinet airflow patterns. Downflow: spray smoke on work surface centerline, 10 cm (4) above sash lower edge, left to right Smoke flow smooth downward, no dead spots or reflux (upward blow) Yes / No
View screen retention: spray smoke 2.5 cm (1) behind sash, 15 cm (6) above sash lower edge, left to right Smoke flow smooth downward, no dead spots, reflux, or escape Work opening edge retention: spray smoke along entire aperture perimeter, 3.8 cm (1.5) outside cabinet Smoke flow smooth, no reflux out once drawn in, no billowing or penetrate over work surface Yes / No Yes / No
Sash wiper seal: spray smoke behind sash, 5 cm (2) from the sides and along the top of work area There shall be no smoke escape from the cabinet Yes / No
Downflow diffuser perimeter: spray smoke at 2.5 cm (1) underneath the diffuser perimeter (Non-NSF test) Smoke flow downward, no dead air corner, no reflux Result Note: The sash wiper seal test requires 2 persons to perform the test: One person to insert hand and eject the smoke One person to check for smoke escape from the cabinet, with front panel lifted IMPORTANT: Return microprocessor to normal mode. If (!) is displayed the cabinet is in Maintenance Mode 1. 2. 3. Use the or button until the LCD displays SET MODE. Press the SET button. Use the or button until the LCD displays NORMAL. Press the SET button. Press the MENU button to exit from the menu tree. Yes / No Pass / Fail
General Information Initial Damper and Voltage Adjustment (Typical values apply only for new BSCs or BSCs with new filters) The recommended initial trial value show as below table:
Cabinet Nominal Inflow Set Point (m/s) Nominal Downflow Set Point (m/s) Blower Voltage (VAC): 1: 230 VAC, 50 HZ 1: 220 VAC, 50HZ 2: 115VAC, 60HZ 3: 230VAC, 60HZ 175 185 104 185 165 165 98 178
AC2-4
0.45 0.32
AC2-6
0.45 0.32
Acceptance criteria / testing procedures have been adopted in accordance with the requirements of the: American Standard NSF/ANSI 49: 2004 Chinese Standard SFDA YY 0569: 2005 European Standard EN12469: 2000 Japanese Standard JIS K 3800: 2005 IEST-RP-CC034.1:2001 Recommended Practice for HEPA and ULPA Filter Leak Tests
1.4 Replacement of Filters 1.4.1 Replacement of Filters for AC2-L BSCs
EXHAUST DAMPER
EXHAUST FILTER CLAMP EXHAUST HEPA FILTER
ACCORN NUT
BLOWER PLENUM
FILTER/BLOWER ACCESS PANEL
FRONT SASH AT FULLY CLOSED DOWNFLOW HEPA FILTER
1. Unscrew and remove exhaust damper. 2. Uncrew and remove the top exhaust filter clamp. 3. Remove the exhaust hepa filter inside. 4. Remove the screws located on 2 sides of blue panel. 5. Open up the hinged front panel which is supported at fixed position by ss hook 6. Remove filter/blower access panel which is held in position by pan head screws m6. 7. Turn the accorn nut of the clamping device on left and right side clockwise, until the blower plenum is moved up. Carefully remove the downflow hepa filter from front side. 8. Replace the filters while reversing the above steps.
Decontaminate before accessing any contaminated plenums
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A. When securing blower housing onto new filter, please ensure gasket compression is equal on both downflow and exhaust filter. B. When securing the exhaust filter mounting frame, please do not over-tighten the nuts, use the lowest torque if using a power tool. Two persons may be required to facilitate filter changing on 5 and 6 BSC. The two ULPA filters under normal usage and barring an accident (a puncture) do not need replacement until the inflow and/or downflow velocity cannot be maintained at the specific rate as mentioned in the test report even though the fan has been set to maximum speed. The filters should not be replaced until the entire BSC has been decontaminated. Please refer to the decontamination procedure Section 1.9.
Fig. 9 Correct Tightening Sequence for the top filter access panel
The BSC must undergo re-certification after the change of filter.
Two steps of tightening are necessary with the first round torque strength of one-third of the final torque required. Over tightening may cause damage to filter and cause leak. 1. Before the filters are removed from the BSC, their contaminated faces should be taped off using plastic film or cardboard or some other suitable material. This will minimize the risk of personnel in the event the decontamination was not thorough. 2. Used filters should be disposed following local regulations. They may have to be incinerated as medical waste. They should be double bagged and appropriately labelled following removal from the BSC. 3. Proper personnel protective attire should be worn when removing used filters (a disposable gown, gloves, facemask, hair and shoe covers are appropriate). These should be disposed after the procedure. Proper hand washing after the procedure is also necessary. 4. Before the new filters are installed, all surfaces should be thoroughly cleaned of silicon and / or adherent gasket material. The new filter should be carefully handled and examined prior to fitting. It is important that the filters and the gaskets be checked for leaks prior to use. The use of non-Esco parts and / or parts not supplied directly by Esco or our authorized distributors, including but not limited to maintenance parts, spare parts, replacement parts, system components and / or system accessories, shall void all expressed or implied warranties.
1.5 Replacement of Blower for AC2-L BSCs 1.5.1 Replacement of Primary Blower
EXHAUST DAMPER
EXHAUST FILTER CLAMP EXHAUST HEPA FILTER BLOWER PLENUM ACCORN NUT
FILTER/BLOWER ACCESS PANEL
FRONT SASH AT FULLY CLOSED
BLOWER
BLOWER PLENUM
1. Unscrew and remove exhaust damper. 2. Uncrew and remove the top exhaust filter clamp. 3. Remove the exhaust hepa filter inside. 4. Remove the screws located on 2 sides of blue panel. 5. Open up the hinged front panel which is supported at fixed position by ss hook 6. Remove filter/blower access panel which is held in position by pan head screws m6. 7. Turn the accorn nut of the clamping device on left and right side clockwise, until the blower plenum is moved up. Carefully remove the downflow hepa filter from front side. 8. Unconnect the blower wiring. 9. Turn the accorn nut of the clamping device blower plenum set to be removed from front side. 10. Remove forward the full assembly blower plenum. 11. Unbolt the blower set and replace 12. Reverse the above steps.
Decontaminate before accessing any contaminated plenums
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1.6 Replacement of Airflow Sensor 1.6.1 Replacement of Airflow Sensor for AC2-L BSCs
Airflow Sensor Temperature Sensor
Cable ties
1. Airflow sensor is located on top of the BSC. Start by disconnecting the airflow sensor cable connector. 2. Cut the cable ties to remove the temperature sensor. 3. Unfasten the nut connecting the airflow sensor to the sensor housing 4. Pull out the old airflow sensor from the sensor housing. 5. Push in the new sensor. Make sure that the arrow sticker on the sensor tube is pointing up as shown in the figure below, so that the axes of the sensor holes are parallel to the airflow direction. Also make sure that the distance between the outer sensor hole and wall of the sensor housing is 5.5cm, as shown in the figure below:
Ensure correct distance
Ensure correct orientation
6. Fasten the nut and reconnect the airflow sensor cable connector. Mount back the cable onto the sensor housing. Make sure that there is no gap between the housing and the filter as shown below:
No gap between the casing and filter
When installing the airflow sensor, please watch the airflow direction
1.7 Replacement of Fluorescent Lamp and UV Lamp 1. Check the replacement parts list at the end of this manual. 2. Disconnect the electrical connections. 3. Remove the two screws from the bottom of blue panel and lift up the blue panel. 4. The fluorescent lamp(s) is located behind the blue panel. Disconnect the old one(s) and replace with the new one(s). Replacement of UV Lamp 1. Check the replacement parts list at the end of this manual. 2. Disconnect the electrical connections. 3. Take out the old UV lamp by rotating it 90o counter clockwise and pull downward. 4. Install the new UV lamp by pushing it upward and rotate 90o clockwise. 1.8 Decontamination Procedure BSC decontamination should be performed in any of the following eventualities: Before replacing the exhaust/downflow filters or blower. Before accessing the contaminated negative or positive plenum of the BSC. In case of an accidental spillage that might have contaminated any of the inaccessible surfaces. Before performance validation and re-certification, especially if the BSC has been used with BSL 3 or 4 agents. Before BSC relocation. Typically decontamination is done with formalin vapor/paraformaldehyde gas, chlorine dioxide gas, or hydrogen peroxide vapor. Formalin and chlorine dioxide are hazardous, so the BSC must be sealed tight to ensure that there is no leakage. In addition, no one shall be present inside the room where the BSC is being decontaminated. Personal protective equipment such as gloves, gown, and respirator with filter suitable for the decontamination gas/ vapor must also be used by the certifier performing the decontamination.
Decontamination should only be performed by qualified personnel
Esco can provide the formalin vaporizer and decontamination bag needed for the decontamination procedure. 1.8.1 Decontamination Agents 1.8.1.1 Formalin/Paraformaldehyde Decontamination Typically the decontamination is performed using formalin gas by either vaporizing 37% formalin solution or by depolymerization of solid paraformaldehyde. Despite its widespread usage for decontamination, formalin presents the following health risks: 1. External contact can cause irritation to skin, eyes, and mucous membranes. 2. Inhalation in small concentrations can cause coughing, nausea, and diarrhea. 3. Inhalation in large concentrations can cause convulsions, coma, and death. 4. Long term exposure can cause cancer.
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Although the Permissible Exposure Level (PEL) for formalin is 0.75 ppm, many scientists believe that there is no safe level of carcinogen exposure to humans. Therefore, typically the room must be evacuated when the decontamination process is performed, which leads to lab down time. The use of formalin decontamination also has other disadvantages: 1. The process is time-consuming. 2. The certifier needs to pulse the BSC fan to circulate the formalin vapor. This can dislodge the tape holding the plastic sheet covering the exhaust filter. 3. Due to excessive residue extensive cleaning must be done after decontamination and before use. The formalin decontamination process can require an extended period of time as outlined below: No. 1 2 3 4 5 6 7 Process
Set-up & sealing the BSC to make it air tight Formalin vaporization Formalin contact time to obtain target log of 4-6 kill Ammonia vaporization to neutralize formalin Ammonia contact time to neutralize formalin Exhausting the ammonia residue Tear-down & cleaning the (substantial) residue TOTAL without ammonia neutralization TOTAL with ammonia neutralization
Time
1 hour hour 8 10 hours hour 2 hours 1 hour 1 hour 10 12 hours 14 17 hours
According to OSHA [Occupational Safety and Health Administration (USA)], formaldehyde Short Term Exposure Level (STEL) is 2 ppm for 15 minutes exposure, 4 times a day, minimum of 60 minutes in between exposures. Any additional local safety regulations should also be observed. Personnel should be given adequate training. The following links provide general guidelines on formaldehyde safety: Regulations (Standards - 29 CFR) Formaldehyde - 1910.1048, Occupational Safety and Health Standards, OSHA (Occupational Safety and Health Administration), U.S. Department of Labor: http://www.osha.gov OSHA Formaldehyde Fact sheet (PDF format) http://www.osha.gov/OshDoc/data_General_Facts formaldehydefactsheet.pdf Ammonia is used to neutralize formaldehyde. OSHA prescribes the ammonia STEL is 35 ppm for 15 minutes exposure, 4 times a day, minimum of 60 minutes in between exposure. Due to the adverse health effect of formalin gas, its use has been banned in Germany, Austria, and Switzerland. Other European countries are expected to follow suit. Two primary candidates to replace formalin decontamination are chlorine dioxide gas and hydrogen peroxide vapor.
1.8.1.2 Chlorine Dioxide Decontamination Chlorine dioxide decontamination is performed by injecting chlorine gas (Cl2) into a cylinder filled with solid sodium chlorite (NaClO2), which generates the greenish-yellow chloride dioxide gas (ClO2). Chlorine dioxide decontamination is much faster than formalin. Being a true gas, it spreads quickly, without the need of pulsing the BSCs blower. It can rapidly kill the micro-organisms with high efficacy with just 1 hour contact time. There is minimal residue to clean after the decontamination making the entire process much faster than formalin decontamination. The time required for the entire process of chlorine dioxide decontamination is as follows: No. 1 2 3 4 5 Process
Set-up & sealing the BSC to make it air tight Chlorine dioxide gassing Chlorine dioxide contact time Chlorine dioxide scrubbing Tear-down & cleaning the (minimal) residue TOTAL
Time
1 hour hour 1 hour hour hour 3 hours
Chlorine dioxide has the PEL of 0.1 ppm, compared to 0.75 ppm for formalin. In both processes airtight BSC sealing is required to protect personnel from the gas exposure. 1.8.1.3 Hydrogen Peroxide Decontamination Hydrogen peroxide (H2O2) decontamination is performed by flash vaporization of an aqueous peroxide mixture, creating a vapor that is distributed throughout the inside the BSC. STERIS and BIOQUELL are two major vendors of hydrogen peroxide generators. There are significant differences in operating principles. The STERIS principle is to avoid condensation on surfaces to minimize corrosion and optimize vapor distribution. The relative humidity inside the BSC must be lowered to 30% so that the remaining 70% relative humidity can be occupied by the hydrogen peroxide vapor. STERIS product for BSC is VHP M100 Biodecontamination System. The BIOQUELL principle is to seek micro-condensation to achieve the kill. The generator releases tiny high-speed droplets inside the BSC. BIOQUELL product for BSC is Clarus L. Hydrogen peroxide vapor is non-carcinogenic, but highly effective against micro-organisms. Hydrogen peroxide (H2O2) vapor breaks down under catalytic action to become air and water, making it environmentally friendly and it leaves no residues. The decontamination process is as fast as chlorine dioxide if the BSC is ducted. However, if the BSC is not ducted, the hydrogen peroxide must be aerated, which is time-consuming. The time needed for the entire process is outlined below: No. 1 2 3 4 Process
Set-up & sealing the BSC to make it semi-airtight Conditioning and decontamination cycle Ducting out H2O2 Tear-down TOTAL 3 hours H2O2 generator doing aeration hour hour 10 hours
Time
hour 1 hour 8 hours
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For hydrogen peroxide decontamination, the BSC need to be equipped with two ports: 1. One port located in front opening or side wall, penetrating the work zone area 2. One port located on top of the exhaust filter. The generator used defines the port function as described below: Steris
Hydrogen peroxide source Bottom front / side port Top port Injected into the BSC Hydrogen peroxide introduction Hydrogen peroxide extraction
Bioquell
Generated inside the BSC Hydrogen peroxide re-introduction Hydrogen peroxide extraction
1.8.1.4 Comparison Table No.

1 2 3 4 5 6 7 8 9 10
Aspect
Is it carcinogenic? Is it a genotoxin? Permissible Exposure Level (PEL) Immediately Damaging to Life & Health (IDLH) Sealing of BSC Lab evacuated during the process? Is room humidity control required? Residue Decontamination time per BSC Equipment cost Yes Yes
Formalin Vapor
No No
Chlorine Dioxide
No No 1 ppm
Hydrogen Peroxide
0.75 ppm 2 ppm Must be airtight Yes, due to leakage danger Yes, above 60% Substantial, needs extensive cleaning 11-17 hours US$ 100 - $2,000 (reusable)
0.1 ppm 5 ppm Must be airtight Yes, due to leakage danger Yes, between 60 to 80 % Minimal, in the form of NaCl 3-4 hours US$1,500 + Cl gas canister (reusable only for several times)
75 ppm Small gaps are OK No, people can still work in lab No No residue. Needs no cleaning at all. 3-11 hours US$18,000 - $52,000 (reusable)
1.8.2 Formalin/Paraformaldehyde Decontamination Process 1.8.2.1 Common List of Equipment Used 1. Device to measure formaldehyde concentration FormaldemeterR 400 has been found to be suitable and can be obtained from: PPM Technology (UK) http://www.ppm-technology.com 2. Device to measure ammonia concentration DragerR-Tubes and AccuroR pumps have been found to be suitable: Dragerwerk AG (Germany) http://www.draeger.com/index.html Tubes with a measuring range of 0-100 ppm are recommended
3. Formalin vaporizer FV-001 Formalin Vaporiser - 220-240 V, 50/60 Hz FV-002 Formalin Vaporiser - 100-130 V, 50/60 Hz Esco Micro Pte. Ltd. http://www.escoglobal.com 4. Spore strips (optional) to verify decontamination efficacy 1.8.2.2 Appropriate Protective Clothing 1. A solid front-back closing, full body, long-sleeved disposable lab gown 2. Disposable shoe covers 3. Disposable surgical/PVC gloves for hand protection NB: Gloves should be pulled over the knitted wrists of the gown rather than being worn inside. Double gloving should be considered. Gloves must be worn while handling formalin and ammonia solutions. Compared to latex gloves, Nitrile gloves exhibit higher resistance to formaldehyde and are less likely to cause allergic skin reactions. Hence Nitrile gloves are preferred over latex gloves. 4. Full-faced mask with a disposable dust and mist respirator. The face shield should be wiped clean with a suitable tissue and water each time after using it.
Full-faced mask
1.8.2.3 Common Apparatus a. Beaker b. Measuring cylinder c. Tape (3M-3939 is recommended) d. Decontamination bag that can enclose the entire BSC (can be ordered from Esco, parts no.: DCN-BAG3) e. Measuring tape f. Biohazard waste bag g. Hand-drill, screwdriver, and socket key (M4 and M5)
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1.8.2.4 Common Preparatory Steps 1. It is recommended that a safety briefing be conducted for all personnel in the laboratory where the BSC has been placed before carrying out the procedure. 2. Access to the laboratory should be restricted during the procedure, especially when youre using formalin vapor/ paraformaldehyde gas and chlorine dioxide gas. A warning sign should be pasted on the door of the lab to warn all personnel. No personnel should enter the lab until the procedure is complete and the decontamination agents concentration has been verified to be within safe limits.
Clearly display warning signs on all doors into the lab
3. Prior to starting the procedure, contingency plans should be drawn for possible incidences of breach in the BSC seal, leading to leakage of decontamination agent into the room. You should properly study the ventilation design of the room and should keep appropriate equipment on standby so the agent can be vented in the event of a leakage. One way of doing this would be to connect a flexible hose to a small exhaust fan which can vent the contaminated air into an adjacent fume hood. Special care should also be taken in case the air in the laboratory is re-circulated to other parts of the building. In such a case, a leakage could even necessitate evacuation of other parts of the building. 4. Set the BSC to maintenance mode (Refer to section 3.8.6 in User Section). In this mode, the technician doing the decontamination is able to turn on the fan to pulse the airflow in the BSC (to distribute the formaldehyde vapor) and also turn on the outlets to supply power to the vaporizers, even when the sash is fully closed. 5. All interior work zones and surfaces of the BSC should be decontaminated with a suitable disinfectant before performing gaseous decontamination.
All sources of hydrogen chloride must be removed from the BSC before decontamination. Hydrogen chloride in the presence of formaldehyde, at ambient air conditions, will form the carcinogen Bis(chloromethyl) ether (BCME).
1.8.2.5 Sealing Methods This article covers the recommended sealing procedures that provide a reasonably good decontaminants containment. The leaktightness may vary, depends on the sealing technique, so the personal protective equipment such as gloves, gown, and respirator with filter that is suitable to the decontamination gas/vapor are still required. 1.8.2.6 Decontamination Bag Method (without ducting) Note: This method is not available for BSC with thimble type exhaust collar due to the BSCs ducting system. 1. Measure the length, width and height of the decontamination bag that would be used to enclose the BSC in order to calculate its volume. If any ductwork is used, the extra volume must also be taken into account for calculating the amount of chemicals that would be needed for carrying out the decontamination. 2. Put the decontamination bag to enclose the entire BSC and support stand.
3. Use the 50 mm wide aluminum tape to seal the gap between the bottom of the decontamination bag and the floor. The tape must be pasted on top of the straightened portion of the decontamination bag. If the tape is pasted on top of crumpled, bent, of flipped decontamination bag, it harder to achieve leaktightness. To compensate for areas where the decontamination bag must turn, use multi section tape. Ensure that the two layers of tape overlap at the end points to prevent leakage.
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4. The overlapped tape at the joints are shown beside:
5. Be careful when there are some joints on the decontamination bag. To ensure that there is no leak coming from the joints, flip the aluminum tape to half so that the adhesive portion is on both sides as shown below. Put this flipped aluminum tape underneath the joint as shown below:
6. After putting the flipped tape underneath the joint, put 3 extra layers of aluminum tape going inward, as shown beside:
7. There are 2 power cables to be used: one for BSC, and one for formalin vaporizer. Do not connect the vaporizer cable to the electrical outlet inside the work zone, because if the building power supply tripped, by the time the power is restored, someone has to press the socket button at the BSC to ON. Therefore, the vaporizer must be connected directly to the building power supply. With this method, after power is restored, the vaporizer will resume the unfinished operation. These 2 cables should be combines so that only 1 cable should come out from the decontamination bag, to minimize chance of formalin leakage.
8. To seal the cable, first make an -shape aluminum tape around the cable, and make a 5 cm flat portion going to both directions like shown beside:
9. Then, paste the aluminum tape to the floor.
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10. Put the bottom part of the decontamination bag on top of the aluminum tape, and then seal it with another layer of aluminum tape. Use multiple layer of aluminum tape (at least 2 layers) on both directions to ensure leak tightness:
11. The BSC is ready for formalin decontamination.
1.8.2.7 Decontamination Bag Method (with ducting) For ducted BSC, you will need to cut a portion of the bag to accomodate the ducting system. The decontamination method it self is basically the same as the non ducted BSC shown above with some additional steps, namely: Close the air tight damper Wrap the bag around the ducting system and seal it using aluminum tape. Use multiple layer of aluminum tape to make sure that it is sealed correctly.
Create a rounded shape using aluminum tape so the adhesive part is on the outside. Use it to put the bag together.
Seal the connection with multiple layers of aluminum tape to ensure its leaktightness.
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1.8.2.8 Decontamination Process There are two alternative combinations of decontaminants and their corresponding neutralizers that are commonly used: 1. 37% formalin solution (HCHO) with 25% ammonia solution (NH4OH) being the neutralizer 2. Paraformaldehyde with ammonium bicarbonate (NH4CO3) being the neutralizer The decontamination processes carried out using these two combinations are slightly different. Both these processes are discussed in details. It is very important to ensure compliance with local laws and regulations with respect to the above mentioned techniques. For instance, if local environmental regulation prohibits the release for formalin vapor into outside environment, the formalin vapor needs to be neutralized by ammonia first, before their byproduct can be exhausted outside. Connecting a temporary duct to the BSC, with the gas being released into a fume cupboard. Connecting a temporary duct to the external atmosphere using a temporary form connection. Using the existing duct connection of the BSC to exhaust the gas (This is applicable only if the BSC is itself ducted). It is very important to ensure compliance with local laws and regulations with respect to the above mentioned techniques. For instance, if local environmental regulation prohibits the release for formalin vapor into outside environment, the formalin vapor needs to be neutralized by ammonia first, before their byproduct can be exhausted outside. 1.8.2.8.1 Formalin Ammonia Combination for Non Ducted BSC
The auto ignition temperature of paraformaldehyde is 300C (572F)
Calculate the amount of 37% formalin, 25% ammonia, and water required, which is 85 ml each, for every 1 cubic meter volume to be decontaminated. Water is poured into the formalin tank to increase the decontamination area humidity to be above 60%. If the room humidity already exceeds 60%, and the formalin tank usage needs to be optimized to decontaminate a large space, such as a small room, then there is no need to add water. Please note that the water shall not be poured into the ammonia tank because it will not assist in the decontamination process. When decontamination bag method is used, the approximate decontamination bag volume that encloses Esco largest Class II Type A2 BSC when mounted on a tall 90 cm (35 in) standing height support stand , and the corresponding requirement for formalin and ammonia, and suggested amount of water are as follows: BSC Width Volume (m )
3
4 ft/1.2 m
3 260 260 260
6 ft/1.8 m
4.3 360 360 360
37% Formalin (ml) 25% Ammonia (ml) Water (ml) - optional
Shorter BSC, especially when installed on a 70 cm (28 in) sitting height support stand, require less formalin, ammonia, and water than the table above. The actual volume to be decontaminated must be measured on site, as it varies from one BSC to another. The figures on the table above are given for comparative purpose only. After approximately 25, 50, 75, and 100% of the formalin being evaporated, turn on the BSC blower for 10 to 15 seconds to circulate the formalin throughout the BSC. Below is the approximate time interval to turn on the blower: BSC Width Volume (m ) decontamination bag method
3
4 ft/1.2 m
3 1.6 10 min
6 ft/1.8 m
4.3 2.3 15 min
Volume (m3) front and top seals method Blower ON interval
In cases where BSC blower is inoperative, circulation of air inside the BSC should be promoted with additional blower or fans, or the time of decontamination should be extended beyond the times suggested below. Allow the formalin vapor a minimum 6 hours (10 to 12 hours is preferable) contact time inside the BSC. Once the formalin decontamination time is reached, start the apparatus containing 25% ammonia to neutralize the formaldehyde. It is recommended to circulate the ammonia by turning on the BSC blower at the same interval as the formalin vaporization. Without any circulation, the neutralization time should be extended to a minimum of 6 hours. Let the ammonia vapor to have a contact time of at least 1 hour (2 hours is preferable) after the solution has been completely evaporated, before opening the seals. Please be aware that the formalin and/or ammonia concentration inside the decontamination bag may be above the STEL and full face mask with formalin filter is required when opening the decontamination bag 1.8.2.8.2 Formalin Ammonia Combination for Ducted BSC Since ducted BSC (with thimbel type collar exhaust) can only use the front and top sealing method, the corresponding requirement for formalin and ammonia, and suggested amount of water are as follows: BSC Width Volume (m )
3
4 ft/1.2 m
1.6 140 140 140
6 ft/1.8 m
2.3 200 200 200
37% Formalin (ml) 25% Ammonia (ml) Water (ml) - optional
The actual volume to be decontaminated must be measured on site, as it varies from one BSC to another. The figures on the table above are given for comparative purpose only. After the formalin has been evaporating for 10 minutes (for 3 & 4 ft BSCs) or 15 minutes (for 5 & 6 ft BSCs), turn on external exhaust blower and slightly open the damper of the exhaust duct for 5 seconds to help the formalin vapor reaches the exhaust filters.
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Allow the formalin vapor a minimum 6 hours (10 to 12 hours is preferable) contact time inside the BSC. Once the formalin decontamination time is reached, start the apparatus containing 25% ammonia to neutralize the formalin vapor. Let the ammonia vapor to have a contact time of at least 1 hour (2 hours is preferable) after the solution has been completely evaporated, before opening the seals. Please be aware that the formalin and/or ammonia concentration inside the decontamination bag may be above the STEL and full face mask with formalin filter is required when opening the decontamination bag. 1.8.2.8.3 Paraformaldehyde Ammonium Bicarbonate Combination for Non Ducted BSC Additional apparatus needed: Heating device (e.g. frying pan) Aluminum foil
Check temperature and humidity. The temperature inside the BSCs work zone should be greater than 210C while the relative humidity should be in between 60% and 85%. Use a hot plate to heat a beaker of water until the desired temperature and humidity are achieved Unless these temperature and humidity conditions are fulfilled, formaldehyde gas will not be effective. Formaldehyde enters living organisms through their cell walls by means of absorption of water
1. Based on the volume obtained in section 1.9.2.8.1, calculate the amount of paraformaldehyde and ammonium bicarbonate needed as follows: Paraformaldehyde amount (gram): Total volume (m3) x 11gram/m3 Ammonium bicarbonate amount (gram): Steps to determine the amount of Ammonium Bicarbonate required: a. Determine the stoichiometric amount of ammonium bicarbonate needed to produce the requisite amount of ammonia gas that would neutralize the resulting formaldehyde gas. b. To ensure completion of the reaction, make sure that the weight of ammonium bicarbonate is 10% greater than the weight of the paraformaldehyde used. 2. Typically, formaldehyde is non-penetrative. So the BSC does not have to be pressure tight. Completely seal the BSC and ensure air-tightness inside the enclosure by using tapes at all appropriate places. Pay special attention to the power supply cables. 3. Wear appropriate protective clothing. 4. Spread the paraformaldehyde evenly over the surface of the heating device. Set the thermostat to 232 C to 246 C (450 F to 475 F).
The auto ignition temperature of paraformaldehyde is 3000 C (5720 F)
5. Another heating device is placed on the work tray for the ammonium bicarbonate. At this stage, the ammonium bicarbonate should be restricted from mixing with the air in the BSC.
Following are two ways of restricting Ammonium Bicarbonate: Method 1: Spread the ammonium bicarbonate evenly over the surface of the heating device and then cover the device with aluminum foil in such a way that ammonium bicarbonate cannot react with formaldehyde while decontamination is in progress. The aluminum foil can be placed in such a way that it allows ammonia gas to escape when the device is heated. Alternatively, some provision can be made for removing the aluminum foil remotely at the beginning of the neutralization phase. Make sure that the deployment of such a removal mechanism doesnt lead to unsafe levels of formaldehyde escaping out of the decontamination bag enclosing the BSC. Method 2: Seal the BSC using a special plastic sheet that has a pair of gloves built into it. The ammonium bicarbonate is placed in a sealed container inside the BSC. Using the built-in gloves, the person performing the decontamination would be able to reach the work zone of the BSC without having to break the seal. At the beginning of the neutralization phase, ammonium bicarbonate is removed from the sealed container and spread evenly over the surface of the heating device. The heating device is then switched on so that ammonia is generated from ammonium bicarbonate. After approximately 25, 50, 75, and 100% of the formalin being evaporated, turn on the BSC blower for 10 to 15 seconds to circulate the formalin throughout the BSC. Below is the approximate time interval to turn on the blower, corresponding to the formalin and ammonia table for the worst case scenario as previously discussed: BSC Width Volume (m ) decontamination bag method
3
4 ft/1.2 m
3 1.6 10 min
6 ft/1.8 m
4.3 2.3 15 min
Volume (m3) front and top seals method Blower ON interval
In cases where BSC blower is inoperative, circulation of air inside the BSC should be promoted with additional blower or fans, or the time of decontamination should be extended beyond the times suggested below. Allow the formalin vapor a minimum 6 hours (10 to 12 hours is preferable) contact time inside the BSC. Once the formalin decontamination time is reached, start the apparatus containing 25% ammonium bicarbonate to neutralize the paraformaldehyde. It is recommended to circulate the ammonia by turning on the BSC blower at the same interval as the formalin vaporization. Without any circulation, the neutralization time should be extended to a minimum of 6 hours. Let the ammonia vapor to have a contact time of at least 1 hour (2 hours is preferable) after the solution has been completely evaporated, before opening the seals. Please be aware that the formaldehyde and/or ammonia concentration inside the decontamination bag may be above the STEL and full face mask with formalin filter is required when opening the decontamination bag.
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1.8.2.8.4 Paraformaldehyde Ammonium Bicarbonate Combination for Ducted BSC After the formalin has been evaporating for 10 minutes (for 3 & 4 ft BSCs) or 15 minutes (for 5 & 6 ft BSCs), turn on external exhaust blower and slightly open the damper of the exhaust duct for 5 seconds to help the formalin vapor reaches the exhaust filters. Allow the formalin vapor a minimum 6 hours (10 to 12 hours is preferable) contact time inside the BSC. Once the formalin decontamination time is reached, start the apparatus containing 25% ammonia to neutralize the formalin vapor. Let the ammonia vapor to have a contact time of at least 1 hour (2 hours is preferable) after the solution has been completely evaporated, before opening the seals. Please be aware that the formaldehyde and/or ammonia concentration inside the decontamination bag may be above the STEL and full face mask with formalin filter is required when opening the decontamination bag. 1.8.3 Completion Steps
1 2
Check the ammonia concentration in room air.
Check the formaldehyde concentration in room air at the end of the decontamination cycle, prior to resuming work on the BSC.
Remove disposable gown and gloves carefully and wash hands using germicidal soap thoroughly.
During this procedure, until the area is determined to be safe, a full face gas mask equipped with a formaldehyde canister, should be worn. The filter for the mask must be selected for the appropriate level of formaldehyde exposure. It must also be replaced regularly following the manufacturers instructions.
Remove the decontamination bag that enclosed the BSC.
Remove all the apparatus from inside the BSC.
Check the formaldehyde and ammonia concentration inside the BSC.
1.8.4 Validation of Decontamination Procedure (Optional) Place some Bacillus subtilis var.niger spore strips inside the BSC prior to decontamination. Work surface/table tray Drain pan Downstream HEPA filter
Place similar strips outside the decontamination room for positive control (optional). Remove all the spore strips and place in Trypticase-soy broth and incubate for 2 days at 37C. If there is no growth of bacteria inside the broth (the broth remains clear), the decontamination procedure has been successfully verified.
Service Section Chapter 2 Troubleshooting
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CHAPTER 2 Troubleshooting
This section helps you troubleshoot some of the common problems you might face while operating this unit should you have any queries left unanswered here, please feel free to contact Esco. 2.1 Diagnostic Read Out 2.1.1 Diagnostic Read Out in Normal Mode Set the hood to normal Mode, then return to original display. Press DOWN to access diagnostic read out
Read Out CP104 Version 1.3 B/H/M TEMP The software name and version. Blower hour meter. Current ambient temperature. Explanation
2.1.2 Diagnostic Read Out in Maintenance Mode Set the hood to Maintenance Mode, then return to original display. Press DOWN to access diagnostic read out
Read Out B/H/M Meter : UV/H/M Meter ADC TEMP Temperature A/F MONITOR ADC IF ADC IFZ ADC IF1 ADC IF2 VEL IFN ADC IFN ADC DF ADC DFZ ADC DFN CONSTANT_1 CONSTANT_2 Blower hour meter. UV hour meter. ADC value from temperature sensor. Current ambient temperature. Show enabled or disabled the alarm. Show actual ADC value from airflow velocity inflow sensor. ADC value from airflow velocity inflow sensor when air velocity is zero (set during zero calibration) . ADC value from airflow velocity inflow sensor at airflow failure point (set during sensor calibration). When the actual ADC value is under the ADC IF1, the alarm must be ON when enabled ADC value from airflow velocity inflow sensor at second inflow point (set during sensor calibration). velocity value of airflow inflow sensor at nominal point (set during sensor calibration) ADC value from airflow velocity inflow sensor at airflow Nominal point (set during sensor calibration). Show actual ADC value from airflow velocity downflow sensor. ADC value from airflow velocity downflow sensor when air velocity is zero (set during zero calibration) . ADC value from airflow velocity downflow sensor at airflow Nominal point (set during sensor calibration). Inflow Sensor constant for airflow velocity sensor (set during sensor calibration). Downflow Sensor constant for airflow velocity sensor (set during sensor calibration). Explanation
82 : Service Section Chapter 2 Troubleshooting
2.2 Electrical and Mechanical Troubleshooting

Problem Possible Cause Corrective Action Check if there is power at the wall/building socket. Power Failure You can use voltmeter or test pen to check power on the wall/ building socket. Ensure the building socket switch is at ON position.
Wall socket
1-1
Cabinet does not start (LCD, button, fan, light and socket are all inoperative)
Check whether power cord has been connected properly into wall/ building socket and also into the unit. Some cabinets have 2 cords. Power cord is not connected properly or faulty Check whether the power cord is giving power, measure the AC voltage between the live and the neutral terminal of the cord by using voltmeter (see Figure 1-3). If the voltage is NOT within +/-2% of the wall socket voltage, replace the cord. If the voltage is within +/-2% of the wall socket voltage, please proceed to next step. IEC Inlet
Cordset Female 1-2 1-3
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If there is power on wall socket and cord, check whether circuit breaker has tripped. To access the circuit breaker, open the front of the cabinet and locate the electrical panel with the RED cover behind it. Circuit breaker has tripped Circuit breaker is located inside this electrical panel. See Figure Layout B at the end of this section to find circuit breaker. Note: If circuit breaker has tripped, do not reset the breaker before checking all electrical components and wiring connections. (see Figure 1-4).
Does the cabinet operate correctly after resetting the circuit breaker? If not proceed to next step.
Push to reset
Circuit breaker (normal condition)
Circuit breaker (tripped condition)
1-4
Lift-up the front panel and locate electrical box (with red metal cover) behind it. Open electrical box and ensure cabinet is plugged in to the main supply. Carefully measure AC voltage between LIVE and NEUTRAL terminal block inside electrical box (See Figure 1-5). Improper connection The voltage should be 230Vac +/- 10%. If the voltage is out of the range, check cable connection at connector A pin 1,2 and 3. At connector A, confirm the voltage is present using the table 1 below. See Figure Layout B at the end of this section to locate connector A and find pin numbering system of the connector.
Connector A Pin Connections 1 2 3
Voltage 230VAC +/-10%
Ground 3
Table 1
Connector A NEUTRAL Terminal LIVE Terminal
1-5
1-6 Check the SMPS (switching mode power supply Model HK-E518A075/120) Input 230VAC +/- 10%. Output_1 +7.5VDC +/- 10% (Red-White cable). Cabinet does not start (LCD, button, fan, light and socket are all inoperative) Output_2 +12VDC +/- 10% (Green-Black cable). Unit must be turned on to perform this test. Defective power supply (SMPS) See Figure Layout B at the end of this section to locate the SMPS. It is inside the electrical panel and covered by a stainless steel box. Checking SMPS output: Disconnect the 5-pin connector attached to the SMPS output then measure the DC voltage between Red (pin 1) and White (pin 2) cables on the SMPS side. See Figure 1-7 below. The voltage should be in range of +7.5VDC +/- 10%. If out of range, please check incoming power to the SMPS Molded cord into SMPS check terminal where the cord is connected. The input of SMPS should be 230Vac +/- 10%. If input is correct but output is not, then replace the SMPS.
Connector at SMPS
SMPS input: 115V AC/230V AC
SMPS output: Red-White= 7.5V DC
Red
Black
1-7
1-8
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See Figure Layout B at the end of this section to locate the main board inside electrical box. Connection problem to main board Measure the incoming voltage on the main board at terminal J 13 (Note polarity, BLUE cable closest to edge is negative -). See Figure 1-9 below to locate terminal J 13. Voltage should be between 6.75 8.25VDC. If voltage is out of range, check connection between SMPS and main board. If voltage is correct, proceed to next step.
Terminal J13 Red-Blue = 7.5VDC
1-9
MAINBOARD
See Figure Layout B at the end of this section to locate the relay board inside electrical box. Connection problem to relay board Measure the incoming voltage on the Relay Board at terminal J 1 (Note polarity, BLUE cable closest to edge is negative -). See Figure 1-10 below to locate terminal J 1. Voltage should be between 6.75 8.25VDC. If voltage is out of range, check connection between SMPS and relay board. If voltage is correct, proceed to next step.
1-10
RELAY BOARD
Restart the cabinet 2 to 4 times. The main board is defective if the main board incoming supply is between 6.75 8.25VDC AND: Defective main board - - - All LEDs on the control panel are off The LCD is blank There is no Buzzer sound
If these conditions exist replace the main board. If these conditions DO NOT exist proceed to next step.
1-11
MAINBOARD LED
LCD
1-12 See Figure Layout B at the end of this section to locate the relay board inside electrical box. Connection problem to relay board Measure the incoming voltage on the Relay Board at terminal J 1 (Note polarity, BLUE cable closest to edge is negative -). See Figure 1-13 below to locate terminal J1. Voltage should be between 6.75 8.25VDC. If voltage is out of range, check connection between SMPS and relay board. If voltage is correct, proceed to next step.
RELAY BOARD
1-13
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Ensure the following are correct: - - - Defective relay board - - main board is operational Flat Ribbon cable is installed correctly to relay board and main board and shows no physical damage Relay board has the correct incoming voltage (6.75 8.25VDC) Check all fuses on relay board: Turn off power, remove fuses and physically inspect or check continuity All wiring connections are good
Turn the cabinet on. If the FAN, LIGHT, SOCKET and UV cannot be controlled, replace the Relay Board. Cabinet does not start (LCD, button, fan, light and socket are all inoperative) Note: When replacing relay board, please re-connect all the cables back correctly. Any wrong wiring may result in damage.
Red-Blue = 7.5VDC Fuse
FRC to Main Board
1-14
Switch on the Fan by pressing Fan button on control panel. Enter the Fan PIN number if required (default is 0001) Fan is Off The LED for FAN should illuminate and the FAN should start. If the FAN does not come on, proceed to next step. If the LED does not illuminate, check connection from control panel to main board or replace the membrane. Blower doesnt function
LED
LCD
2-1
Switch off the cabinet. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the relay board inside electrical box. Check Fuse F1 on relay board, see Figure 2-2 below. If fuse F1 is blown, as temporary solution, use F5 to replace F1. The fuse F5 is not used and is a spare fuse. If fuse F1 is okay, proceed to next step.
Fuse F1 is blown
Fuse F1
2-2 Blower doesnt function Switch on the blower on the cabinet. Open electrical box and see Figure Layout A or B at the end of this section to locate the motor speed controller-Accuflow G2 . It is attached on the electrical box cover. Check AC line LED indicator lights. LED indicator lights signify that the voltage and frequency detected by the Accuflow G2. Follow indicator LED lights guide below for the diagnosis of the Accuflow G2: 1. No output, LED indicators off - Accuflow G2 is not on. Check 12Vdc SMPS input cable to Accuflow G2 if 12 Vdc input is present. Refer to Figure 2-3 for reference. If 12Vdc input to Accuflow G2 is present, Accuflow G2 is defective. Replace with new one. Accuflow G2 has not been programed correctly and hasnt passed factory test. Return the Accuflow G2 to be tested or replace it with a new one. Power line voltage is fluctuating or not within the acceptable range. Stable acceptable range for Accuflow G2 is (90-143 V or 198-264 V). Power line frequency is fluctuating or not within the acceptable range. Make sure that the AC power supply frequency is stable and within the acceptable range (47-53 Hz or 57-63 Hz). The output voltage is locked at the preset level. Slide the LOCK/ ADJUST switch to the ADJUST position, then make the necessary adjustment on the blower voltage.
- Faulty motor speed controller Accuflow G2
2. No output, LED indicators blink -
3. No output, both 230 and 115 Vac line voltage indicator LED are on -
4. No output, both 50 and 60Hz line frequency indicator LED are on -
5. The voltage output cannot be adjusted -
: 89
6. The voltage output cannot be adjusted - The output voltage is locked at the preset level. Slide the LOCK/ ADJUST switch to the ADJUST position, then make the necessary adjustment on the blower voltage. If the LOCK/ADJUST switch doesnt work, check if there is cable connection to the RMT port. The Accuflow G2 may be controlled remotely thought this port. If no connection on RMT port, Accuflow G2 will need to be replaced.
If you followed the steps outlined above and still the blower doesnt work, Switch off the cabinet and proceed to next steps below. Add jumper cables between the 2 cables Live and Neutral(jumper Neutral-Input to Neutral-Output and Live-Input to Live-output) going to Accuflow G2. See Figure 2-3 below. Switch on the cabinet. If the fan operates properly, you can conclude that the Accuflow G2 is defective and needs to be replaced. If the fan does not operate, the problem is not with the Accuflow G2, proceed to next step.
To 12VDC SMPS
Blower doesnt function
To motor
2-3 Switch the unit off. See Figure Layout B at the end of this section to locate the capacitor inside electrical box. See Figure 2-4 below, disconnect two cables of capacitor. Using a capacitance meter or a Voltmeter set to capacitance measurement, record the value between two capacitor cables. Faulty capacitor Correct value is 23-27F. If the value is out of given ranges, replace capacitor. If the capacitance is within the given ranges proceed to next step. Warning: The capacitor may still have some electrical charges if the cabinet was on prior doing this checking. Wait for 1-2 minutes and check the capacitor voltage using a voltmeter to ensure the capacitor were discharged (voltage less than 5Vac) prior disconnecting the capacitor.
Disconnect 2 cables from capacitor. Measure capacitors capacitance using multimeter.
Capacitor
2-4
Turn off the Cabinet Check LS1 relay on the Relay Board. See Figure 2-4 below. Make sure all wiring and connections are correct. On terminal J 2, check the NO to Common and NC to Common circuits by checking continuity. If the NO to Common and NC to Common contacts are not correct, replace the Relay Board. If the NO to Common and NC to Common contacts are correct, proceed to the next step. Check K1 relay, see Figure Layout B at the end of this section to locate the K1 relay. With cabinet still switched-off, check if the cables connected to the relay socket are tight. See Figure 2-5 below. Visually inspect the relay for burnt contacts or flash marks on the inside of the relay case. (Relay is the clear case component) Check NO to Common and NC to common contacts on relay K1, the method is same as for LS1 relay. Blower doesnt function If the relay K1 shows signs of arching (burnt marks) or the NO-C and NC-C circuits are not correct, replace the relay.
Faulty relays
Terminal J2
Relay LS1
C NO NC
2-4
Check connection
Check for burnt mark Check connection
230VAC relay
2-5
: 91
Check the Fan for overheating The motor has a thermal cut out built-in. Auto-thermal cut-off Blower doesnt function Wait 60 minutes with the FAN turned off and then try to restart. If the FAN restarts determine why there is excessive heat in the cabinet. Proceed to next step. Warning: Decontamination must be done on the cabinet before accessing the fan.
Motor failure
Check whether the motor can rotate properly and not loosened. Check for physical damage. If present, instruction on how to replace the blower is available in this manual under Service Section. Ensure that there are no external sources of airflow disturbance like air conditioner vent, window or incidences of door opening or people walking fast near the cabinet. Locate airflow sensor installed inside the work zone. Ensure there is no air disturbance or obstruction around the sensor. If there is no external air interference, proceed to next step. Ensure that the front and back air grilles inside the cabinet are not blocked and that the cabinet is not excessively loaded. See figure 3-1. If there is no blocked air grille, proceed to next step.
External air interference
Blocked cabinet grille
Airflow Failure (AIRFAIL) - alarm is triggered Turn on the cabinet.
3-1
Open the front panel and locate electrical box behind it. Open electrical box and find motor voltage sampling port as shown on Figure 3-2. Low building supply voltage ((if new cabinet, check this first) For new cabinet, if the motor voltage is lower than the Nominal Voltage value recorded in factory test report, adjust the Accuflow G2 to get output voltage to blower as specified in the Test Report Test Conditions Documentation Sheet. For cabinet that has been used for more than 1 year, refer to last motor Nominal Voltage from last certification report or if not available, measure the actual airflow on cabinet and increase motor voltage accordingly to achieve nominal airflow velocity. If supply voltage is same as Nominal Voltage, proceed to next step.
3-2
Plug in the voltmeter to the Motor Voltage Sampling Port. Measure the actual airflow velocity using anemometer or flowhood. If actual airflow is okay but LCD still shows AIRFAIL, proceed to next step. If actual airflow is not okay, please adjust motor Accuflow G2 accordingly until alarm is turned off and the LCD shows nominal airflow reading. After this, if LCD still shows AIRFAIL, proceed to next step. The sensor will take about 2-3 minutes before it can register a new airflow setting. Note: The cabinet must be certified at least annually. If the blower is already operating at maximum voltage and the airflow alarm is still triggered due to filter loaded, its time to change the filter. If filter is not loaded, proceed to next step. Re-calibrate the microprocessor. Follow the calibration procedure available in the factory test report. Warning: To be carried out only by an authorized person. CHECKING CALIBRATION DATA After calibration is done, go to MENU, key in PIN if required, choose SET MODE, choose MAINTENANCE. LCD will blink in MAINTENANCE MODE. Go to main display by pressing MENU button twice. Airflow Failure (AIRFAIL) - alarm is triggered Press SET button, LCD will display software version that is currently being used on the cabinet. Example: CP104 Version 1.3 Press DOWN button until you find below message: - - - - Faulty/ inaccurate calibration - - ADC TEMP: ADC value from temperature sensor. TEMP : this is the ambient temperature read by temperature sensor (in C) A/F MONITOR: this is the airflow sensor monitoring alarm whether enabled or disabled. ADC IF : Show actual ADC value from airflow velocity inflow sensor. ADC IFZ: ADC value from airflow velocity inflow sensor when air velocity is zero (set during zero calibration). ADC IF1: ADC value from airflow velocity inflow sensor at airflow failure point (set during sensor calibration). when the actual ADC value is under the ADC IF1, the alarm must be ON when enabled ADC IF2: ADC value from airflow velocity inflow sensor at second inflow point (set during sensor calibration). VEL IFN: velocity value of airflow inflow sensor at nominal point (set during sensor calibration) ADC IFN: ADC value from airflow velocity inflow sensor at airflow Nominal point (set during sensor calibration). ADC DF: Show actual ADC value from airflow velocity downflow sensor. ADC DFZ: ADC value from airflow velocity downflow sensor when air velocity is zero (set during zero calibration). ADC DFN: ADC value from airflow velocity downflow sensor at airflow Nominal point (set during sensor calibration). CONSTANT_1: Inflow Sensor constant for airflow velocity sensor (set during sensor calibration).
Filter loaded or wrong Accuflow G2speed controller setting
- - - - - - -
: 93
CONSTANT_2: Downflow Sensor constant for airflow velocity sensor (set during sensor calibration).
In order to prevent any AIRFAIL or unstable velocity displayed on LCD problem, please ensure the following is done after the calibration: 1. CONSTANT value is correct. Check it with constant written on sensor body or inside Replaceable Component Record List which come with test report. Faulty/ inaccurate calibration 2. TEMP shows room temperature. If not, refer to Problem 3 Possible cause 7 Part B. 3. ADC IFZ < ADC IF1 < ADC IF2. If not please re-do calibration in correct sequence: SET CONSTANT SET ZERO CALIB. If CALIBRATION ERROR is encountered, please refer to Problem 3 Possible cause 7 Part A. 4. ADC DFZ < ADC DFN. If not please re-do calibration in correct sequence: SET CONSTANT SET ZERO CALIB. If CALIBRATION ERROR is encountered, please refer to Problem 3 Possible cause 7 Part A. A. Air Flow Sensor failure / misalignment There are 2 units of airflow sensor, exhaust and downflow. Exhaust airflow sensor is located on top of the unit, above exhaust filter and installed inside white metal box. See Figure 3-3 below. It is a stainless steel tube with 2 round holes facing up. Downflow airflow sensor is located below the downflow filter, inside cabinet work zone Important to check: Airflow Failure (AIRFAIL) - alarm is triggered 1. Ensure that the sensor area is not blocked at all and that its throughholes are perpendicular to the filters surface. 2. Exhaust sensor is attached tightly to the box. 3. There is no gap between sensor box and filter surface. 4. Sensor box is installed tightly to the cabinet. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the main board inside electrical box. Sensor Failure / Sensor Misalignment Using multimeter, check the exhaust sensors output voltage (DC) at channel AIN1(+) and J1(-) on main board (See Figure 3-4 below). Check also the downflow sensors output voltage (DC) at channel AIN2(-) and J2(+) on main board. The exhaust sensor voltage should increase if exhaust motor voltage (N2) is increased and decrease if exhaust motor voltage is decreased. The downflow sensor voltage should increase if downflow motor voltage (N1) is increased and decrease if downflow motor voltage is decreased. If your observation is different, check exhaust sensor connection on top of the unit (see Figure 3-5 below) for any bad/loose connection. If connection is okay but sensor is still not working properly, replace it. If airflow sensor is working properly, proceed to next step. B. Temperature Sensor Failure There is only 1pc temperature sensor. Temperature sensor is located on top of the unit, close to airflow sensor, and attached on the white metal box. See Figure 3-6. Using multimeter, check the temperature sensors output voltage (DC) at channel AIN4(-) and J4(+) on main board (See Figure 3-4 below).
0.01VDC should represent one degree Celsius. For example, if output is 0.25VDC, it indicates temperature of 25 degree C. Tolerance of +/1 degree C is acceptable. Replace temperature sensor if the reading is wrong. If temperature sensor is working properly, proceed to next step.
Temperature sensor output Airflow sensor output
Airflow Failure (AIRFAIL) - alarm is triggered
3-3
3-4
Temperature sensor
3-5
3-6 Check the motor. If motor is not running, refer to Blower doesnt function problem. If motor is working properly, proceed to next step. Blocked air grille Make sure that the front and back air grilles are not blocked. See Figure 3-1. Adjust the Accuflow G2 (located inside the electrical panel, behind the front panel) and / or damper (if present, located on top of the unit, above exhaust filter) to get the optimum airflow as stated in the test report. Decontaminate the cabinet and change the downflow filter. Re-certify the cabinet after the new filter has been installed. Ensure that there are no external sources of airflow disturbance like air conditioner vent, window or incidences of door opening or people walking fast near the Cabinet.
Motor failure
Low airflow Contaminated samples Leaking downflow filter
Environmental air disturbance
: 95
Exhaust filter is located on top of the unit. Patch the leaking filter with 3M DP-100 glue. Contaminated lab Leaking exhaust filter The patched area should not exceed 4% of the total filter area. Then check the filter for leakage again. If the filter is still leaking, decontaminate the cabinet and change the exhaust filter. Re-certify the cabinet after the new filter has been installed. Lift-up the front panel and locate electrical box (with red metal cover) behind it. Open electrical box and see Figure Layout A or B at the end of this section to locate the Accuflow G2 inside electrical box. Resonance Increase the motor speed by 5 10 VAC. Check whether the noise disappears. If the noise is already gone, measure the actual airflow velocity. If the velocity is still in the acceptable range, keep it. If velocity is out of acceptable range, or if noise persists, go to next step.
Refer to above Blower doesnt function --> Faulty Capacitor section, to check the capacitor, and replace if necessary. Loosed motor or impeller wheel mounting Warning: For next step, decontaminate the unit before accessing to blower. To be carried out only by an authorized person.
Excessive fan noise
After decontamination, open the blower access panel (see Figure 6-1 below) and check if the blower mounting bolts have been fully tightened. Also check whether the motor can rotate properly and not loosed. If motor is physically damaged, replace it.
6-1
Turn off the cabinet. Open the front panel and locate electrical box behind it. Blank LCD Connection problem Open electrical box and see Figure Layout B at the end of this section to locate the main board inside electrical box. Check whether LCD FRC (Flat Ribbon Cable) has been inserted properly into its socket on the main board. See Figure 7-1 below.
LCD FRC cable
7-1
Turn off the cabinet. Contrast problem Adjust the potentiometer on main board by rotating the top metal part using flat screw driver to achieve the best LCD contrast. Anti clock-wise direction will increase the contrast. See Figure 7-2 below. If the LCD remains blank, replace it.
Potentiometer
7-2 Blank LCD

MAIN BOARD
Connect a new LCD to the LCD port on main board (refer to possible cause 1 above on how to find the LCD port). Defective LCD If the new LCD functions properly, means the old one is Defective. Replace it. If the new LCD is not working, check its cable or replace the main board.
LCD FRC cable
7-3
: 97
Turn off the cabinet. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the main board inside electrical box. See Figure 8-1 below and ensure FRC cable going to interface board is connected properly. The triangle sign on the female connector indicates PIN number 1. Connection problem Interface board and membrane/keypad are located behind the blue panel, underneath the light metal deflector. With the cabinet still turned-off, uninstall the fluorescent light and metal deflector to access the interface board. See Figure 8-2 below for the proper connection between main board, interface board, and membrane/keypad. Check if the green plastic cable from the membrane has been inserted properly into the interface board. Follow Figure 8-3 for the correct orientation of connector.
FRC to main board
Inoperative buttons
INTERFACE BOARD
Green plastic cable
MEMBRANE
8-2 8-1
8-3
: 99
Inoperative buttons
Defective Cable and / or Interface Board and / or keypad
Replace them one by one, to check which one(s) among them is/are Defective. Replace the Defective part(s). Move the sash to Ready position (normal operating height). Ready position is indicated by black dot mark on the right profile cover (see Figure 9-1 below).
Sash in SASH ALARM or UV state
Switch on the light by pressing LIGHT button on the membrane/ keypad. Light can only be ON at Ready or Fully Open sash position. If light cannot be ON when sash at Ready position, proceed to next step.
9-1 Faulty fluorescent tube Replace the faulty fluorescent tube. Fluorescent tube is located inside the blue panel (see Figure 9-4). Turn off the cabinet. Open electrical box and see Figure Layout B at the end of this section to locate the fluorescent ballast, connector C, and relay board inside electrical box. Turn on the cabinet, then turn on the light by pressing LIGHT button on membrane/keypad. Check AC voltage at ballast input (between pin NO on J13 terminal on relay board to neutral), see Figure 9-5 below. It should be 230Vac +/- 10% for 230V cabinet. If not, check the LS7 relay and F7 fuse (refer to possible cause 5 below). Turn off the unit. Disconnect male connector C on electrical box. Turn on the unit. Turn on the light, then check AC voltage at ballast output (between female connector C pin 1 and 2), it should be around 450-600Vac. Replace ballast if output voltage is much lower than this range.
Light always OFF
Faulty Fluorescent ballast
9-2
Turn off the cabinet. Open electrical box and see Figure Layout B at the end of this section to locate the relay board, fluorescent ballast, and connector C inside electrical box. Connection Problem Check for any loosed or bad connection between relay board, fluorescent lamp ballast, and female connector C. See Figure 9-3 to locate terminal J 13 on relay board, check tightness of 2 cables connected to J 13. For connector C, disconnect the male side from electrical box, then check male and female side for any bad connection. Check also connection of the lamp holder (see Figure 9-4).
Fluorescent lamp tube
Fluorescent lamp holder
9-3 Light always OFF Turn off the cabinet.
9-4
Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the relay board inside electrical box. Check relay LS7 and fuse F7 on the relay board. See Figure 9-5 below. . Faulty relay or fuse If fuse F7 is blown, as temporary solution, use F5 to replace F1. The fuse F5 is not used and is a spare fuse. On terminal J13, check the NO to Common and NC to Common circuits by checking continuity. If the NO to Common and NC to Common contacts are not correct, replace the Relay Board. If the NO to Common and NC to Common contacts are correct, proceed to the next step.
9-5
: 101
Lower the sash to UV mode position (fully closed, see Figure 10-1 below). Sash not in UV state If UV MODE is not displayed on the LCD, please refer to the Magnetic Switch troubleshooting section. Switch on the UV lamp by pressing the UV button. If UV MODE is displayed on LCD but UV is not on after pressing UV button, please refer to the Magnetic Switch troubleshooting section.
10-1 Faulty UV light Replace the faulty UV light. Note: typical UV light life is 8000 hours. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the UV ballast, relay board, and connector B inside electrical box. UV Light always OFF Turn on the cabinet, fully close the sash. Then switch on the UV by pressing UV button on membrane/keypad. Check AC voltage at UV ballast input (between pin NO on J15 terminal on relay board to neutral), see Figure 10-3. Faulty UV ballast It should be 230Vac +/- 10%. If not, check the LS8 relay and F8 fuse (refer to possible cause 4 below). Turn off the unit. Disconnect male connector C on electrical box. Turn on the unit. Turn on the UV, then check AC voltage at ballast output (between female connector C pin 6 and 12 ). It should be around 10-25Vac for 230Vac. Replace ballast if output voltage is out this range. Turn off the cabinet. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the relay board inside electrical box. Check LS8 relay and fuse F8 on the relay board. See Figure 10-2 below. Faulty relay or fuse If fuse F8 is blown, as temporary solution, use F5 to replace F8. The fuse F5 is not used and is a spare fuse. On terminal J 15, check the NO to Common and NC to Common circuits by checking continuity. If the NO to Common and NC to Common contacts are not correct, replace the Relay Board. If the NO to Common and NC to Common contacts are correct, proceed to the next step.
10-2 Turn off the cabinet. Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the relay board, UV ballast, and connector C for 230Vac cabinet inside electrical box. UV Light always OFF Connection Problem Check for any loosed or bad connection between relay board, UV lamp ballast and female connector B . See Figure 10-3 to locate terminal J 15 on relay board, check tightness of 2 cables connected to J 15. For connector B , disconnect the male side from electrical box, then check male and female side for any bad connection. Check also connection of the UV lamp holder (see Figure 10-4).
10-3
10-4
: 103
Turn off the cabinet. Open electrical box and see Figure Layout B at the end of this section to locate the relay board and connector B for 230Vac cabinet inside electrical box. Connection Problem Check for any loosed or bad connection between relay board and female connector B. See Figure 11-2 to locate terminal J 6 on relay board, check tightness of 2 cables connected to J 6. For connector B, disconnect the male side from electrical box, then check male and female side for any bad connection. Check also connection on electrical socket, it is located inside the work zone (see Figure 11-1 below).
11-1 Turn off the cabinet. Electrical Socket always OFF Open the front panel and locate electrical box behind it. Open electrical box and see Figure Layout B at the end of this section to locate the relay board inside electrical box. Check LS4 relay and fuse F4 on the relay board. See Figure 11-2 below. Faulty relay or fuse If fuse F4 is blown, as temporary solution, use F5 to replace F4. The fuse F5 is not used and is a spare fuse. On terminal J 6, check the NO to Common and NC to Common circuits by checking continuity. If the NO to Common and NC to Common contacts are not correct, replace the Relay Board. If the NO to Common and NC to Common contacts are correct, proceed to the next step.
11-2
Socket is located inside the work zone See Figure 11-2. Faulty electrical socket Check electrical socket connection. Refer to Figure 11-3 below. If connection is correct but socket has no output, replace electrical socket.
Socket 230V cabinet
Green/Yellow Screw
E L N
Electrical Socket always OFF
11-3 Front View (cover opened) Red or Black Blue Color Rear View (removed)
Layout A. Accuflow G2 Speed Controller Layout
: 105
Layout B. Component Layout
Layout C. Relay Board
Layout D. Main board
: 107
2.3 Sash Position Detection Esco BSCs use magnetic-switches in order to help the control system keep track of the position of the sash window. The way this tracking is done can be better understood from the following diagram and table.
Magnet Mag. Switch 1 Magnet
Sash Window (front view)
Mag. Switch 2
Mag. Switch 4
Mag. Switch 3
Sash movement range
Fig. 21 Sash Position Detection
The lower side of sash is able to be moved in the range shown in left side. Three magnetic switches are installed in the right side while one magnetic switch is installed on left side. While sash window moving up/down, magnetic switches will be switched on/off depends on the position of 2 Magnet strips that are installed on the sash window. The principle is: Magnetic Switch 1
Close Open Open Open
Magnetic Switch 2
Open Close Open Open
Magnetic Switch 3
Open Open Close Open
Magnetic Switch 4
Open Close Open Open
Description
Sash is fully opened, ALARM is given. ALARM can be muted. Sash is fully closed; UV can be operated. BSC sash is at nominal height, ready to use. SASH is at unsafe state, ALARM is given. ALARM can not be muted. ERR.MSWITCH detected
Rest Conditions
There is a basic principle of sash position detection, 2 or more magnetic switches are not supposed to be closed at the same time (except for Magnetic switch 2 and 4). If the situation raised, user must check the condition of magneticswitch. If displayed message is not matching the sash position, please check the position of respecting magnetic switch by opening front panel, and removing sash profile if needed.
2.4 Operation Mode Summary Sash Position Function Available

Sash Position Detection
Normal Mode and Airfail Alarm On

Yes Yes, with WARM UP &
Normal Mode and Airfail Alarm Off

Yes Yes, with WARM UP & PURGING time, FAN PIN required Yes Yes Interlocked Yes, ADMIN PIN required Simple Diagnostic Message Yes Yes No, FAN ON displayed
Maintenance Mode
No Yes, with WARM UP & PURGING time, no PIN required Yes Yes Yes, no UV timer Yes, no PIN required Complete Diagnostic Message No No, (!) displayed Yes
Fan Control Light Control Socket Control UV Control Ready MENU Access Pressing Set button Timer Current Time Display Air Velocity Display
PURGING time, FAN PIN required Yes Yes Interlocked Yes, ADMIN PIN required Simple Diagnostic Message Yes Yes Yes
If not calibrated, displaying ERR.CALIB Air Fail checking Sash Position Detection Message Displayed Current Time Display Alarm Sash Alarm Fan Control Light Control Socket Control UV Control Yes, Alarm if air fails Yes SASH ALARM Yes Yes, not mutable Yes, with WARM UP & PURGING time, FAN PIN required No Yes Interlocked No Yes SASH ALARM Yes Yes, not mutable Yes, with WARM UP & PURGING time, FAN PIN required No Yes Interlocked Not Applicable Not Applicable Not Applicable Not Applicable No Not Applicable Not Applicable Not Applicable Not Applicable
Sash Position
Function Available
Sash Position Detection Message Displayed Current Time Display Alarm
Normal Mode and Airfail Alarm On

Yes FULLY OPEN Yes Yes, mutable for 5 minutes Yes, with WARM UP & PURGING time, FAN PIN required Yes Yes Interlocked
Normal Mode and Airfail Alarm Off

Yes FULLY OPEN Yes Yes, mutable for 5 minutes Yes, with WARM UP & PURGING time, FAN PIN required Yes Yes Interlocked
Maintenance Mode
Not Applicable Not Applicable Not Applicable Not Applicable
Fully Open Fan Control Light Control Socket Control UV Control
Not Applicable Not Applicable Not Applicable Not Applicable
: 109
Sash Position Detection SWITCH OFF Fan requested Message Displayed UV MODE Fan Control Light Control Socket Control UV Control
Yes Yes UV MODE No No Yes Yes
Yes Yes UV MODE No No Yes Yes
Not Applicable Not Applicable Not Applicable Not Applicable Not Applicable Not Applicable Not Applicable
2.5 Software Troubleshooting Error Codes Explanation Corrective Action

Check for Faulty Magnetic Switch(es). Press MENU button and key in ADMIN password (if any). Pressing SET button to read diagnostic message. Interpreting the stages of the three Magnetic Switches Switch_0 (J3) ON when sash is fully opened, there is a 7.5 V at J3 port. Switch_1 (J10) ON when sash is at nominal height, the DIN1 and J10 are shorted. Switch_2 (J11) ON when sash is fully closed, the DIN2and J11 are shorted. If all Switches are off, the sash is at SASH ALARMposition. Check the position of switch and signal at main board respe tively (magnetic switches can be found after removing sash profile). Remove any magnetic devices near the BSC. Check for faulty main board of Sentinel control Switch off the system. Remove the connection from J3, DIN1, J10, DIN2, and J11. Switch on the system. If the LCD does not show SASH ALARM, the main board is faulty. Check for inflow velocity using anemometer. If the inflow actually drops below the fail point (see Calibration section for exact number), adjust the speed control to achieve blower output voltage as stated in Calibration certificate. Warning: this step should be carried by an authorized person. If the inflow still above the fail point, Press menu and key in ADMIN password to switch to MAINTENANCE mode. Press SET button to read diagnostic message. Check whether Constant and Temperature are correct. If Constant is not correct, please change constant to factory setting. If temperature is incorrect, please check the wire connection and temperature sensor. Call your distributor to replace defective parts if any. Check the wire connection and air-flow sensor. Call your distributor to replace defective parts if any. Warning: this step should be carried by an authorized person. Calibrate the controller. Refer to Calibration section for details. Warning: this step should be carried by an authorized person.
ERR. MSWITCH
MAGNETIC switch error: This error happened when there are more than 1 Switch is activated.
ERR. AIRFAIL
Airflow Error: happens if FAN button is pressed when airflow falls below fail point. Switch on FAN again and wait it to be stable before troubleshooting
ERR. CALIB
Controller is not yet calibrated
Service Section Chapter 3 Product Specification
: 111
CHAPTER 3 Product Specification

3.1 Airstream Max Model AC2 (L-Series) Engineering Details
1400 mm (55.1")
7 10 8
3 4
760 mm (29.9")
11 5 12 13
965 / 1270 / 1575 / 1880 mm (37.9" / 50" / 62" / 74") 1035 / 1340 / 1645 / 1950 mm (40.7" / 52.7" / 64.7" / 76.7")
9
560 mm (22") 733 mm (28.8")
1. Exhaust ULPA filter 2. Blower 3. Downflow ULPA filter 4. Standard IV bar Retrofit Kit provision 5. Plugged service fixture provisions (2 on each side) 6. Electrical and electronics panel 7. Standard UV light Retrofit Kitprovision 8. Fluorescent lamp 9. Stainless steel armrest 10. Esco Sentinel microprocessor control system 11. Tempered glass sliding sash window 12. Standard electrical outlet Retrofit Kit provision 13. Stainless steel multi-piece work tray
112 : Service Section Chapter 3 Product Specification
3.2 AC2-L Series General Specifications
General Specifications, Airstream Max Class II, Microbiological Safety Cabinets (L-Series)
Note to customer: Insert electrical voltage number into last model number digits _ when ordering Model Nominal Size External Dimensions (W x D x H) Without Base Stand With Optional Base Stand, 711mm (28") type AC2-4L__ 1.2 meters (4') 1340 x 740 x 1413 mm 52.8 x 29.1 x 55.6 1340 x 740 x 2124 mm 52.8 x 29.1 x 83.6 1270 x 560 x 670 mm 50.0 x 22.0 x 26.4 0.58 m2 (6.2 sq.ft) 0.45 m/s (90 fpm) at initial setpoint 0.32 m/s (63 fpm) at initial setpoint with uniformity of better than +/- 20% 356 m3 /h ( 209 cfm) 790 m3 /h (465 cfm) 356 m /h (209 cfm)
3
AC2-6L__ 1.8 meters (6') 1950 x 740 x 1413 mm 76.7 x 29.1 x 55.6 1950 x 740 x 2124 mm 76.7 x 29.1 x 83.6 1880 x 560 x 670 mm 74.0 x 22.0 x 26.4 0.87 m2 (9.3 sq.ft)
Internal Work Area, Dimensions (W x D x H) Internal Work Area, Space Average Airflow Velocity Inflow Downflow Inflow Airflow Volume Downflow Exhaust ULPA Filter Typical Efficiency Downflow Exhaust
527 m3 /h (310 cfm) 1096 m3 /h (645 cfm) 527 m3 /h (310 cfm)
>99.999% at 0.1 to 0.3 microns and MPPS as per IEST-RP-CC001.3 USA with H14 rating as Exhaust per EN 1822, Europe <58 dBA >1250 Lux (>116 foot candles) <60 dBA 1250 Lux (>116 foot candles)
Typical Sound Emission per EN 12469 Fluorescent Light Intensity At Zero Ambient Main Body Cabinet Construction Work Zone Side Walls 220-240V, AC, 50Hz, 1 Cabinet Power/ Amp Electrical** Outlet Amp Fuse Total Amp BTU/ Hr Net Weight*** Shipping Weight*** Shipping Dimensions, Maximum (W x D x H)*** Shipping Volume, Maximum***
1.2 mm (0.05) 18 gauge electrogalvanized steel with white oven-baked epoxy Isocide antimicrobial powder coated finish 1.5 mm (0.06) 16 gauge stainless steel, type 304, with 4B finish UV absorbing tempered glass, 5 mm (0.2), colorless and transparent AC2-4L1 550 W/ 2.5 A 5A 7.5 A 1122 258 kg (585 lbs) 313 kg (710 lbs) 1500 x 950 x 1800 mm 59.0 x 37.4 x 70.8 2.57 m3 (91 cu.ft.) AC2-6L1 650 W/ 3.5 A 5A 8.5 A 1326 300 kg (661 lbs) 354 kg (780 lbs) 2100 x 950 x 1800 mm 82.7 x 37.4 x 70.8 3.59 m3 (127 cu.ft.)
* Noise reading in open field condition/ anechoic chamber. ** Additional voltages may be available; contact Esco for ordering information. *** Cabinet only; excludes optional stand
For Microbiological Safety Cabinets*

Standards Compliance
For Air Quality

ISO 14644.1 Class 3, Worldwide
For Filtration
IEST-RP-CC034.1, Worldwide IEST-RP-CC007.1, Worldwide IEST-RP-CC001.3, Worldwide EN 1822 (H14), Europe
For Electrical Safety

IEC 61010-1, Worldwide EN 61010-1, Europe UL 61010-1, USA CAN/ CSA C22.2 No. 61010-1
EN 12469, Europe
IEST-G-CC1001, USA IEST-G-CC1002, USA
Service Section Chapter 3 Product Specification
: 113
3.3 Environmental and Electrical Requirements The BSC has been designed to be used under the following conditions: Environment Requirements
Europe / Asia / Australia Situation Relative Humidity Altitude Temperature Pollution Degree Indoor Use Only 20% - 90% Up to 2000 meters (6600 ft) Temperature should be in between 18 degree C and 30 degree C (65 deg F and 86 deg F). 2.0 *
* Pollution degree describes the amount of conductive pollutants present in an operating environment. In Pollution degree 2, it is assumed that only non-conductive pollutants such as dust are present, except that occasional conductivity is caused by condensation. ** Installation category (over voltage category) defines the level of transient over voltage that the instrument is designed to Electrical Requirements
Europe / Asia / Australia Power Rating Power Source Maximum Voltage Fluctuation Installation Category Surge Protection and UPS Leakage Current Voltage dips & short interruptions immunity test 220-240V AC 50Hz Dedicated with unobstructed access 10% of nominal voltage Category II ** Strongly recommendedz All electrical outlets combined should not exceed 2.0mA At the time of carrying out the Voltage dips & short interruptions immunity test, flickers were observed in the flourescent light and UV light whenever the voltage dips and short interruptons were applied on the AC mains port of the BSC. This condition got rectified automatically after the test. The BSC is considered to meet performance criteria B in Voltage dips & short interruptions immunity test based on the above observation.
withstand safely. It depends on the nature of electricity supply and the BSCs over voltage protection means. CAT II is a category used for instruments which can be plugged to a power point which is comparable to the public mains found in facilities such as hospitals, research laboratories and industrial laboratories. The expected transient over voltage in CAT II is 2500 V for a 230 V supply and 1500 V for a 120 V supply.
APPENDIX
Replacement Parts List

The use of non-Esco parts and / or parts not supplied directly by Esco or our authorized distributors, including but not limited to spare parts, system components and / or system accessories, shall void all expressed or implied warranties.
NO
ITEM/CABINET MODEL
FILTER AC2-4L1
ITEM CODE
DESCRIPTION
QTY
MECHANICAL REPLACEMENT PARTS

1 EQR/SP-FTR103 EQR/SP-FTR72 AC2-6L1 EQR/SP-FTR125 EQR/SP-FTR126 2 BLOWER AC2-_L1 3 GLASS AC2-4L1 AC2-6L1 AC2-_L1 AC2-_L1 4 TUBULAR MOTOR AC2-_L1 EQR/ME-TBR-MOTOR-1 TUBULAR MOTOR 220V, 50/60HZ WITH METAL DRIVE ADAPTOR AND ACCESSORIES 1 EQR/GL-64 EQR/GL-66 EQR/GL-129R EQR/GL-129L TEMPERED GLASS GL 64 (FRONT SASH) TEMPERED GLASS GL 66 (FRONT SASH) TEMPERED GLASS NO 129R (SIDE GLASS) TEMPERED GLASS NO 129L (SIDE GLASS) 1 1 1 1 EQR/EL-BLOWER-11 BLOWER NO. 11 1 (4 ft), 2 (6 ft) ULPA FILTER NO. 103 ULPA FILTER NO. 72 ULPA FILTER NO. 125 ULPA FILTER NO. 126 1 1 1 1
ELECTRICAL REPLACEMENT PARTS

1 ELECTRICAL MODULE AC2-4L1 AC2-6L1 1.1 SPEED CONTROL AC2-_L1 AC2-_L1 1.2 BALLAST AC2-_L1 1.3 UV BALLAST AC2-_L1 1.4 RELAY AC2-_L1 AC2-_L1 AC2-_L1 EQR/EL-REL-JQX30A EQR/EL-REL-JQX220V EQR/EL-REL-JQX12VD RELAY 30A/120V RELAY 10A/2POLE, 220-240V RELAY 10A/2P WITH PROTECTION DIODE 1 1 1 EQR/EL-BAL-EBP30 UV BALLAST EBP-30 1 EQR/EL-BAL-UNV-T8-IS ELECTRONIC BALLAST 1 EQR/EL-MC-25A EQR/EL-BLOWER-45 ACCUFLOW SPEED CONTROLLER REV 3 BLOWER NO. 45 1 (4 ft), 2 (6 ft) 1 (4 ft), 2 (6 ft) EP-CP2-AC2-4L1 EP-CP2-AC2-6L1 ELECTRICAL PANEL FOR AC2-4L1 COMPLETE WITH SPEED CONTROLLER 230V ELECTRICAL PANEL FOR AC2-6L1 COMPLETE WITH SPEED CONTROLLER 230V 1 1
2 2.1
AIRFLOW ALARM AND CONTROL SYSTEM PANEL STICKER OVERLAY AC2-_L1 EQR/EL-MC-30 MEMBRANE SWITCH FOR C+CONTROL 1
2.2
MAINBOARD AC2-_L1 EQR/EL-MC-18 MAIN BOARD (C-SERIES) 1
2.3
RELAY BOARD AC2-_L1 EQR/EL-MC-19A 7 RELAY BOARD ( C-SERIES ) 1
2.3.1
FUSE AC2-_L1 EQR/EL-FUSE-F5AL250V 5A QUICK ACTING F5AL250V FUSE 5
2.4
INTERFACE BOARD AC2-_L1 EQR/EL-MC-20 INTERFACE BOARD ( C-SERIES ) 1
2.5
LCD AC2-_L1 EQR/EL-MC-35 BIG LCD MODULE 1
2.6
SMPS AC2-_L1 EQR/EL-MC-22 SMPS 1
2.7
AIRFLOW SENSOR AC2-_L1 AC2-_L1 EQR/EL-SA-B1 EQR/EL-SA-B2 AIR FLOW SENSOR REV. B1 AIR FLOW SENSOR REV. B2 1 1
2.8
TEMPERATURE SENSOR AC2-_L1 EQR/EL-SA-TEMP TEMPERATUR SENSOR 1
CAPACITOR AC2-_L1 EQR/EL-CCAP-25MF Capacitor, 400V, 25 mf 1 (4 ft), 2 (6 ft)
FLUORESCENT TUBE AC2-6L1 AC2-4L1 EQR/EL-TUB-3F25W EQR/EL-TUB-4F32W FLUORESCENT TUBE 3FT FLUORESCENT TUBE 4FT 4 2
UV TUBE AC2-_L1 EQR/EL-TUB-UVG30T8 UV TUBE 3FT 1
CIRCUIT BREAKER AC2-4L1 AC2-6L1 EQR/EL-CB-2 EQR/EL-CB-3 CIRCUIT BREAKER 4A CIRCUIT BREAKER 6A 1 1
MAGNETIC SWITCH AC2-_L1 EQR/EL-MC-BS2011-UL MAGNETIC CONTACT WITH UL LOGO 3
MAGNETIC AC2-_L1 EQR/EL-MC-MAGNETIC MAGNET WITH ADHESIVES 2
Notes: Please indicate Serial Numbers upon ordering the parts to replace.
Further Information and Reference Materials

The following documents are available for download at www.escoglobal.com, in the Technical Support area. These documents have been selected as pertinent to this product and we would recommend that anyone operating the BSC familiarize themselves with this information. 1. 2. 3. 4. 5. INTRODUCTION TO BIOHAZARD SAFETY CABINET BIOLOGICAL SAFETY LEVELS CLASSIFICATION KI-DISCUS TEST ULTRAVIOLET LAMPS IN LAMINAR FLOW AND BIOLOGICAL SAFETY CABINETS EXHAUST DUCTED CLASS II, TYPE A2 SAFETY CABINETS
Filtration Technology Introduction to Contamination Control and Cleanroom Technology. 2000. Matt Ramstorp. Wiley-VCH. Weinheim. Cleanroom Technology. Fundamentals of Design, Testing, and Operation. 2001. Whyte.W. Wiley, West Sussex, England. IEST-RP-CC001: HEPA and ULPA Filters.2002. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-RP-CC007: Testing ULPA Filters.2002. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-RP-CC021: Testing HEPA and ULPA Filter Media.2002. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-RP-CC034: HEPA and ULPA Filter Leak Tests.2002. Institute of Environmental Sciences and Technology, Illinois, USA. BS EN 13091: Biotechnology. Performance Criteria for Filter Elements and Filtration Assemblies. 2000. British Standards, UK.
Clean Air Technology ISO 14644: Cleanrooms and Controlled Environments .2000. International Organization for Standardization, Switzerland. IEST-G-CC1001: Counting Airborne Particles for Classification and Monitoring of Cleanrooms and Clean Zones.1999. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-G-CC1002: Determination of the Concentration of Airborne Ultrafine Particles.1999. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-G-CC1003: Measurement of Airborne Macro Particles.1999. Institute of Environmental Sciences and Technology, Illinois, USA. IEST-G-CC1004: Sequential Sampling Plan for Use in Classification of the Particulate Cleanliness of Air in Cleanrooms and Clean Zones.1999. Institute of Environmental Sciences and Technology, Illinois, USA.
Biological Safety Cabinets BS EN 12741: Biotechnology. Laboratories for Research, Development, and Analysis. Guidance for Biotechnology Laboratory Operations. 1999. British Standards, UK. BS EN 12128: Biotechnology. Laboratories for Research, Development, and Analysis. Containment Levels of Microbiology Laboratories, Areas of Risk, Localities, and Physical Safety Requirements. 1998. British Standards, UK. Anthology of Biological safety I. Perspectives on Laboratory Design.1999. Richmond, J.Y., American Biological Safety Association. Illinois, USA. Anthology of Biological safety II. Facility Design Consideration.1999. Richmond, J.Y., American Biological Safety Association. Illinois, USA.
Anthology of Biological safety III. Application of Principles.1999. Richmond, J.Y., American Biological Safety Association. Illinois, USA. BS EN 12469: Biotechnology. Performance Criteria for Microbiological Safety Cabinet. 2000. British Standards, UK. BS EN 12296: Biotechnology. Equipment. Guidance on Testing Procedures for Cleanability. 1998. British Standards, UK. BS EN 12297: Biotechnology. Equipment. Guidance on Testing Procedures for Sterilizability. 1998. British Standards, UK. BS EN 12298: Biotechnology. Equipment. Guidance on Testing Procedures for Leak Tightness. 1998. British Standards, UK. National Sanitation Foundation Standard Number 49 for Class II (Laminar Flow) Biohazard Cabinetry. 2002. National Sanitation Foundation, Michigan, USA. AS2252.2: Class II Biological Safety Cabinets. 1994. Australia. AS2567: Cytotoxic Drug Safety Cabinets. 1994. Australia. AS2639: Cytotoxic Drug Safety Cabinets. Installation and Use. 1994. Australia. AS2647: Biological Safety Cabinets. Installation and Use. 1994. Australia. JIS K3800: Class II Biological Safety Cabinets.2000. Japan. SABS 0226: Installation, Post-Installation Tests and Maintenance of Microbiological Safety Cabinets. 2001. South Africa.
Websites 1. www.escoglobal.com Esco Micro Pte. Ltd. 2. www.nsf.org NSF International 3. http://www.hc-sc.gc.ca/hpb/lcdc/biosafty/docs/index.html Health Canada - Laboratory Biological safety Guidelines 4. http://www.cdc.gov/od/ohs/biosfty/bsc/bsc.htm Centre for Disease Control Primary Containment for Biohazards 5. http://www.cdc.gov/od/ohs/biosfty/bmbl4/bmbl4toc.htm Centre for Disease Control Office of Health and Safety Biological safety in Microbiological and Biomedical Laboratories 6. http://www.osha.gov Occupational Safety and Health Administration, USA 7. http://www.absa.org American Biological Safety Association 8. http://www.cetainternational.org and http://www.cetainternational.org/links.htm Controlled Environment Testing Association and Related Links
Log Record
Installed By: Date : _____________________________________________ Company: _________________________________________ Responsible Person: ________________________________ Cabinet Model: __________________________________ Serial Number: ___________________________________
1. This log record should be used by the operator to record any new agents / bacteria / viruses that has been introduced to the cabinet during its operation, problems encountered, etc. 2. Any decontamination procedure performed by either the user or the technician should be recorded down as well. 3. Please also record any major maintenance procedure performed by the service technician, for example: filter changing, recertification, UV lamp replacement, etc.
Date
Event
User signature
Supervisor signature
Esco Technologies, Inc. 2940 Turnpike Drive, Units 15-16 Hatboro, PA 19040, USA Toll-Free USA and Canada 877-479-ESCO Tel 215-441-9661 Fax 215-441-9660 us.escoglobal.com usa@escoglobal.com Esco Micro Pte. Ltd. 21 Changi South Street 1 Singapore 486 777 Tel. +65 6542 0833 Fax +65 6542 6920 www.escoglobal.com mail@escoglobal.com
Esco Global Offices | Kuala Lumpur, Malaysia | Leiden, The Netherlands | Manama, Bahrain Mumbai, India | Philadelphia, USA | Salisbury, UK | Shanghai, China | Singapore
2009 Esco Micro Pte. Ltd. Specifications Subject to Change. ES1319_V4_03/09

AC2-L User Service Manual Version 5.1 (10 May 2010)

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Esco Controlled Environment Laboratory and Cleanroom Equipment Solutions

Class ll Biological Safety Cabinets

User and Service Manual

User And Service Manual

Class II Biological Safety Cabinet User and Service Manual

Declaration of Conformity............................................................................................................. ix About Esco Biological Safety Cabinets......................................................................................... xi

1.3 Airflow Pattern Inside the BSC....................................................................................................................3

2.4 Performance Validation / Certification.....................................................................................................13

3. Operating Your BSC. ......................................................................................................................15

3.3 Starting the BSC..........................................................................................................................................18

3.4 Working in the BSC. ....................................................................................................................................19

3.7 Menu Options.............................................................................................................................................25

4. Maintaining Your BSC............................................................................................................. 39

Class II Biological Safety Cabinet User and Service Manual

1.5 Replacement of Blower for AC2-L BSCs....................................................................................................62

1.6 Replacement of Airflow Sensor. ................................................................................................................63

2.1 Diagnostic Read Out. ..................................................................................................................................81

3. Product Specification............................................................................................................ 111

Class II Biological Safety Cabinet User and Service Manual

Warranty Terms and Conditions

Warranty Terms and Conditions

Warranty Terms and Conditions

Class II Biological Safety Cabinet User and Service Manual

Electrical 220-240V, AC, 50Hz, 1

1.8 meters (6)

Electrical Hazard: Danger of electric shock

Approved Service Engineer Only: Operation to be performed only by approved engineer

Class II Biological Safety Cabinet User and Service Manual

Lim Lay Yew Chief Executive Officer

* Applicable only to 220-240V, AC, 50 Hz cabinets

Class II Biological Safety Cabinet User and Service Manual

About Esco Biological Safety Cabinets

Bio Safety Level (BSL)

About Esco Biological Safety Cabinets

Protection Offered Operator

Min. Inflow Velocity

Recirc. Exhaust Air Air

Contaminated Plenum Surrounded By

Inside room / Hard Duct

Class II Biological Safety Cabinet User and Service Manual

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 1 Basic Product Information

chapter 1 Basic Product Information

1. The first label will contain:

2. The second label will contain:

User Section Chapter 1 Basic Product Information

1.2 Quick View 1.2.1 Airstream Max Model AC2-L

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 1 Basic Product Information

Dynamic air barrier, inflow and forward-directed downflow air converge

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 2 Installation

User Section Chapter 2 Installation

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 2 Installation

User Section Chapter 2 Installation

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 2 Installation

10 : User Section Chapter 2 Installation

Class II Biological Safety Cabinet User and Service Manual

User Section Chapter 2 Installation

1. Install the exhaust collar (Figures 1, 2, and 3):

Fig. 1 Exhaust collar, with slots only in front and back